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U.S. Meat Animal Research Center Clay Center, Ne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mission

Research Units

U.S. Meat Animal Research Center Animal Health Research Unit

U.S. Meat Animal Research Center Environmental Management Research Unit

U.S. Meat Animal Research Center Genetics & Breeding Research Unit

U.S. Meat Animal Research Center Meat Safety and Quality Research Unit

U.S. Meat Animal Research Center Nutrition Research Unit

U.S. Meat Animal Research Center Reproduction Research Unit

Classical Biological Control For The Protection Of Natural Ecosystems, R.G. Van Driesche, R I. Carruthers, T. Center, M.S. Hoddle, J. Hough-Goldstein, L. Morin, L. Smith, D.L. Wagner, B. Blossey, V. Brancatini, R. Casagrande, C.E. Causton, J.A. Coetzee, J. Cuda, J. Ding, S.V. Fowler, J. H. Frank, R. Fuester, J. A. Goolsby, M. Grodowitz, T.A. Heard, M.P. Hill, J.H. Hoffmann, J. Huber, M. Julien, M.T.K. Kairo, M. Kenis, P. Mason, J. Medal, R. Messing, R. Miller, A. Moore, P. Neuenschwander, R. Newman, H. Norambuena, W.A. Palmer, R. Pemberton, A. Perez-Panduro, P.D. Pratt, M. Rayamajhi, S. Salom, D. Sands, S. Schooler, M. Schwarzländer, A. Sheppard, R. Shaw, P.W. Tipping, R.D. Van Klinken

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Of the 70 cases of classical biological control for the protection of nature found in our review, there were fewer projects against insect targets (21) than against invasive plants (49), in part, because many insect biological control projects were carried out against agricultural pests, while nearly all projects against plants targeted invasive plants in natural ecosystems. Of 21 insect projects, 81% (17) provided benefits to protection of biodiversity, while 48% (10) protected products harvested from natural systems, and 5% (1) preserved ecosystem services, with many projects contributing to more than one goal. In contrast, of the 49 projects against invasive …

Coronavirus Replicase Proteins: Multifunctional Mediators Of Replication And Innate Immunity Evasion, Mark Anthony Clementz

Dissertations

Coronaviruses are positive-sense, single-stranded RNA viruses. The majority of the RNA encodes non-structural proteins (nsps) that are translated as a large polyprotein, which is cleaved by the papain-like (PLP) and picornavirus 3C-like (3CLpro) proteases. The nsps modify host membranes to produce double membrane vesicles (DMVs) upon which the replicase-transcriptase assembles and synthesizes viral RNA. nsp3, nsp4, and nsp6 are integral membrane proteins believed to be involved in DMV formation. Work presented here demonstrates that nsp4 is subjected to N-linked glycosylation and mutation of N258 to threonine in nsp4 confers a temperature sensitive phenotype to MHV-A59 infectious clone virus. This virus …

Nutrient Removal As A Function Of Corn Stover Cutting Height And Cob Harvest, Jane M. F. Johnson, Wally W. Wilhelm, Douglas L. Karlen, David W. Archer, Brian J. Wienhold, David T. Lightle, David Laird, John Baker, Tyson E. Ochsner, Jeff M. Novak, Ardell D. Halvorson, Francisco Arriaga, Nancy Barbour

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

One-pass harvest equipment has been developed to collect corn (Zea mays L.) grain, stover, and cobs that can be used as bioenergy feedstock. Nutrients removed in these feedstocks have soil fertility implication and affect feedstock quality. The study objectives were to quantify nutrient concentrations and potential removal as a function of cutting height, plant organ, and physiological stage. Plant samples were collected in 10-cm increments at seven diverse geographic locations at two maturities and analyzed for multiple elements. At grain harvest, nutrient concentration averaged 5.5 gN kg⁻¹, 0.5 gP kg⁻¹, and 6.2 gK kg^{−1 …}

Repellency Of A Wax-Based Catnip-Oil Formulation Against Stable Flies, Junwei J. Zhu, Christopher A. Dunlap, Robert W. Behle, Dennis R. Berkebile, Brian J. Wienhold

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Stable flies, Stomoxys calcitrans (L.), are one of the most serious livestock pests, which cause significant economic loss in the cattle industry. Current practices for managing stable flies are limited to costly sanitation techniques and unsustainable insecticide applications. The present study reports the initial efforts using catnip essential oil as a spatial repellent and the results of field trials using a wax-based formulation to repel stable flies in the cattle feedlot. Electroantennograms showed that catnip oil and its ingredient compounds elicit significant antennal responses from both sexes of stable flies. Catnip oil and ZE- and EZ-nepetalactone showed repellent …

Immunogenicity And Reactivity Of Novel Mycobacterium Avium Subsp. Paratuberculosis Ppe Map1152 And Conserved Map1156 Proteins With Sera From Experimentally And Naturally Infected Animals, John Bannantine, Avery L. Paulson, Ofelia Chacon, Robert J. Fenton, Denise Zinniel, David S. Mcvey, David R. Smith, Charles J. Czuprynski, Raúl G. Barletta

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Mycobacterium avium subsp. paratuberculosis causes Johne’s disease (JD) in ruminants. Development of genetic tools and completion of the M. avium subsp. paratuberculosis genome sequencing project have expanded the opportunities for antigen discovery. In this study, we determined the seroreactivities of two proteins encoded at the 5􏰋 and 3􏰋 regions of the MAP1152-MAP1156 gene cluster. MAP1152 encodes a PPE protein, and MAP1156 encodes a diacylglycerol acyltransferase involved in triglyceride metabolism and classified in the uncharacterized protein family UPF0089. Recombinant MAP proteins were overproduced and purified from Escherichia coli as maltose-binding protein (MBP) fusions. Immunoblotting analysis indicated that both MAP1152 and MAP1156 …

Primary Transcriptomes Of Mycobacterium Avium Subsp. Paratuberculosis Reveal Proprietary Pathways In Tissue And Macrophages, Harish K. Janagama, Elise A. Lamont, Sarah B. George, John P. Bannantine, Wayne W. Wayne, Zheng J. Tu, Scott J. Wells, Jeremy Schefers, Suelee Robbe-Austerman

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Mycobacterium avium subsp. paratuberculosis (MAP) persistently infects intestines and mesenteric lymph nodes leading to a prolonged subclinical disease. The MAP genome sequence was published in 2005, yet its transcriptional organization in natural infection is unknown. While prior research analyzed regulated gene sets utilizing defined, in vitro stress-related or advanced surgical methods with various animal species, we investigated the intracellular lifestyle of MAP in the intestines and lymph nodes to understand the MAP pathways that function to govern this persistence.

Results: Our transcriptional analysis shows that 21%, 8% and 3% of the entire MAP genome was represented either inside tissues, …

Threadfin Shad Impacts Phytoplankton And Zooplankton Community Structures In Channel Catfish Ponds, Bartholomew W. Green, Peter Perschbacher, Gerald Ludwig, Sara E. Duke

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plankton community structure and chlorophyll a concentration were compared in 12 0.1-ha earthen ponds co-stocked with channel catfish (Ictalurus punctatus Rafinesque, 1818) in multiple-batch culture (initial biomass = 5,458 kg ha^-1) and a planktivore, threadfin shad (Dorosoma petenense Güther, 1867; initial biomass = 449 kg ha^-1), during the April-November growing season. We used a completely randomized design in 2 x 2 factorial arrangement to test planktivore level (presence or absence of threadfin shad) and channel catfish feeding frequency (daily or every 3^rd d). Channel catfish were fed a 32% protein feed to apparent …

Misappropriation Of Shuar Traditional Knowledge (Tk) And Trade Secrets: A Case Study On Biopiracy In The Amazon, Winston P. Nagan, Eduardo J. Mordujovich, Judit K. Otvos, Jason Taylor

UF Law Faculty Publications

Where the murkiness of biopiracy as a general matter leaves little room for legal theory to anchor, the relative clarity of specific instances of biopiracy may provide sufficient factual information from which to develop appropriate legal theories. In particular, the way biopiracy has been used to misappropriate the traditional knowledge (TK) of the Shuar Nation of Ecuador suggests that there may be legal theories for which the process of misappropriation may give rise to liability under international law as well as under developments in the domestic laws of the United States and Ecuador. The possible efficacy and legal coherence of …

The Sitting Ducks Of Securities Class Action Litigation: Bio-Pharmas And The Need For Improved Evaluation Of Scientific Data, Stuart R. Cohn, Erin M. Swick

UF Law Faculty Publications

Rule 10b-5, a powerful weapon against any publicly-listed company whose share price drops on adverse news, is particularly skewed against pharmaceutical and other bio-technology companies (bio-pharmas). It is not a coincidence that there is a disproportionate number of class actions filed against bio-pharmas. The volume and complexity of data underlying most bio-pharma cases create enormous outcome uncertainties, settlement pressures, and potentially huge contingent liabilities over substantial periods of time. The vulnerability and risks that bio-pharmas face in Rule 10b-5 class actions are unique among all publicly-traded industries, yet many cases proceed along traditional grounds without courts employing either their statutory …

Effects Of Forage Quality And Cell Wall Constituents Of Bermuda Grass On Biochemical Conversion To Ethanol, William F. Anderson, Bruce S. Dien, Hans-Joachim G. Jung, Kenneth P. Vogel, Paul J. Weimer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Bermuda grass is an attractive candidate as a feedstock for biofuel production because over four million hectares of Bermuda grass are already grown for forage in the Southern USA. Because both rumen digestion and biochemical conversion to ethanol depend upon enzymatic conversion of the cell wall polysaccharides into fermentable sugars, it is probable that grasses bred for increased forage quality would be more amenable for ethanol production. However, it is not known how variation in rumen digestibility and cell wall/fiber components correlates with efficiency of conversion to ethanol via fermentation. The objective of this research was to determine relationships between …

Biorational Approaches To Managing Stored-Product Insects, Thomas W. Phillips, James E. Throne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Stored-product insects can cause postharvest losses, estimated from up to 9% in developed countries to 20% or more in developing countries. There is much interest in alternatives to conventional insecticides for controlling stored-product insects because of insecticide loss due to regulatory action and insect resistance, and because of increasing consumer demand for product that is free of insects and insecticide residues. Sanitation is perhaps the first line of defense for grain stored at farms or elevators and for food-processing and warehouse facilities. Some of the most promising biorational management tools for farm-stored grain are temperature management and use of natural …

The Not-So-Risky Business Of High-End Escorts And The Internet In The 21st Century, Robert Rigg

Richmond Journal of Law & Technology

Prostitution is often referred to as “the world’s oldest profession.” It is defined as “[t]he act or practice of engaging in sexual activity for money or its equivalent.” As a service industry, it fulfills specific human desires. Nevertheless, most states condemn and criminalize both the act of prostitution and those who solicit such services.

The Truth About Haiti, Irwin P. Stotzky

Articles

No abstract provided.

Combining A Dispersal Model With Network Theory To Assess Habitat Connectivity, Todd R. Lookingbill, Robert H. Gardner, Joseph R. Ferrari, Cherry E. Keller

Geography and the Environment Faculty Publications

Assessing the potential for threatened species to persist and spread within fragmented landscapes requires the identification of core areas that can sustain resident populations and dispersal corridors that can link these core areas with isolated patches of remnant habitat. We developed a set of GIS tools, simulation methods, and network analysis procedures to assess potential landscape connectivity for the Delmarva fox squirrel (DFS; Sciurus niger cinereus), an endangered species inhabiting forested areas on the Delmarva Peninsula, USA. Information on the DFS’s life history and dispersal characteristics, together with data on the composition and configuration of land cover on the peninsula, …

Extractive Reserves, David S. Salisbury

Geography and the Environment Faculty Publications

Extractive reserves are territories dedicated to environmental protection and the sustainable use of nature resources by traditional populations. Reserves follow a traditional land tenure model based on individual family and communal property rights to common areas, such as forest trails used to extract or harvest nontimber forest products. Although the extractive reserve concept originates in the tropical forests of the Brazilian Amazon, reserves have also been created in aquatic, floodplain, and savanna landscapes throughout Brazil. There are now 50 extractive reserves covering more than 10 million hectares, an area larger than Portugal, and more continue to be created. Despite their …

Civil Evidence, Luis G. Zambrano

SMU Law Review

No abstract provided.

Taxation, Cynthia M. Ohlenforst, Sam Megally, Angela Johnson

SMU Law Review

No abstract provided.

Patenting The Human Body: The Constitutionality Of Gene Patents And Suggested Remedies For Reform, Olga Bograd

SMU Law Review

No abstract provided.

North Dakota Supreme Court Review, North Dakota Law Review Associate Editors

North Dakota Law Review

No abstract provided.

The Law Of Unintended Consequences: The North Dakota Supreme Court Recognizes The Right To A Jury Trial For Noncriminal Traffic Offenses In Riemers V. Eslinger, Peter Welte

North Dakota Law Review

No abstract provided.

John F. Kennedy History, Memory, Legacy: An Interdisciplinary Inquiry, John Delane Williams, Robert G. Waite, Gregory S. Gordon

Open Educational Resources

On September 25, 1963, President John F. Kennedy traveled to Grand Forks, North Dakota, greeted its citizens while touring the city, and delivered a speech at the University of North Dakota Field House, which addressed important issues still vital today: environmental protection, conservation of natural resources, economic development, the struggle between democracy and totalitarianism, and the importance of education and public service. The University conferred on the President an honorary Doctor of Laws degree. Over 20,000 people assembled on campus that day to see JFK -- the largest campus gathering in UND history. Tragically, less than two months later, the …

Molecular Identification Of Arowana Species Based On Cytochrome B And Cytochrome C Oxidase I (Coi) / Maryam Zahedi Fard, Maryam Zahedi Fard

Student Works (2010-2019)

Mitochondrial DNA (mtDNA) markers has been widely used to better resolve the taxonomic identification as well as display the genetic relatedness of different species and closely related fish. The highly cost ornamental fish, Asian arowana (Scleropages formosus) of family Osteoglossidae has different colored strains that are geographically distributed in different regions around Southeast Asia. The taxonomic classification of these different strains still remains unclear and disputable. The partial sequences of the cytochrome b and the DNA barcoding gene, cytochrome C oxidase were analyzed and assessed for molecular identification; to differentiate the colored varieties of this species. The results of this …

Picturing Family Histories: Torill Kove And Lene Ask, Melissa Gjellstad

Languages & Global Studies Faculty Publications

No abstract provided.

A Micro-Transponder For Precision Tracking Of Underwater Targets, Albert Whitaker Franzheim Iii.

Master's Theses and Capstones

A prototype micro-transponder has been designed and built to track, in real-time, the positions of objects or animals within the water column. Commercially available data storage tags, which help monitor the behavior of underwater animals, do not provide real-time interrogation and data dissemination capabilities in a form factor and acoustic frequency band acceptable for active tracking applications.

This prototype is 18.5 cm3 and weighs 43.7 g in air. It operates at an acoustic frequency of 160 kHz and uses a mixed-signal topology with low-power components and a microcontroller, which allows for firmware updates and addition of external sensors. It is …

Truncated Mortalin In Animal Cancer, Katrina K. Olson

Master's Theses and Capstones

Hematopoetic neoplasia or clam hemocyte cancer (a leukemia-like disease) has been studied in a number of bivalve molluscs for the last 20 years. Recent molecular studies of the hemocytes of the soft shell clam, Mya arenaria, have demonstrated an interaction between p53 and mortalin, the mitochondrial Hsp70. The former protein is intimately involved in the initiation of cell-cycle arrest, apoptosis, DNA repair, and cell differentiation. In cancerous clams, wild-type p53 is sequestered in hemocyte cytoplasm by mortalin and cannot be translocated to the nucleus. This is critical because although p53 is functions properly, it is unable to enter the nucleus …

Landscape Genetics Of The New England Cottontail: Effects Of Habitat Fragmentation On Population Genetic Structure And Dispersal, Lindsey E. Fenderson

Master's Theses and Capstones

The New England cottontail (Sylvilagus transitionalis) is a species of conservation concern. Population recovery will require knowledge of genetic structure and dispersal patterns. To this end, I used microsatellite loci to assess genetic structure at two spatial scales: across the entire range (broadscale) and within the northernmost population (finescale).

Range-wide, cottontails are separated into five distinct populations. There was little evidence of gene flow among populations and they have experienced extensive genetic drift. Several populations had comparatively reduced genetic diversity.

Intensive fine-scale surveys revealed four genetically differentiated populations. Interstate-95 is a dispersal barrier, though other major roads did not impact …

Aerosol Extinction Measurements With A New Multipass Aerosol Differential Optical Absorption Spectrometer (A-Doas): Laboratory Validation And Initial Ambient Measurements, Ryan T. Chartier

Master's Theses and Capstones

Atmospheric aerosols directly affect climate by scattering and absorbing incident solar radiation. The magnitude of the impact is dependent upon the wavelength of incoming light, but is typically approximated near 550 nm. As a result, climate models can have large uncertainties for aerosol contributions when considering the solar spectrum. An aerosol differential optical absorption spectrometer (A-DOAS) has been developed to address to measure aerosol extinction from mid-ultraviolet to near infrared. The instrument consists of a closed UV-Vis spectrometer coupled to a White-type multi-pass gas cell with an adjustable path length of up to 20 meters. Laboratory extinction measurements of polystyrene …

Genomic Resource Development For A Diploid Mint: Mentha Longifolia, Zahra Hadadian

Master's Theses and Capstones

This research project aimed to develop genomic resources needed to enable construction of a genetic linkage map of the diploid mint species Mentha longifolia. Such a map would facilitate identification of plant genes involved in resistance to Verticillium fungal infection. For this purpose, a small genomic library was constructed from germplasm accession CMEN 585, 279 genomic inserts were sequenced and annotated and 19 PCR primer pairs were designed and tested on two resistant and two susceptible accessions. The Cleaved Modified Polymorphic Sequence (CAPS) method of molecular marker genotyping was found to detect little variation between crossing parents CMEN 585 (resistant) …

Using Voice To Tag Digital Photographs On The Spot, Michael A. Farrar

Master's Theses and Capstones

Tagging of media, particularly digital photographs, has become a very popular and efficient means of organizing material on the internet and on personal computers. Tagging, though, is normally accomplished long after the images have been captured, possibly at the expense of in-the-moment information. Although some digital cameras have begun to automatically populate the various fields of a photograph's metadata, these generic labels often lack in the descriptiveness presented through user-observed annotations and therefore stress the necessity of a user-driven input method. However, most mobile annotation applications demand a great number of keystrokes in order for users to tag photographs and …