Digital Commons Network^™

Construction And Expression Of Anti-Cd62p/Anti-Gpiib-Iiia Diabody, De-Jie Chen, Zui Tan

Saudi Medical Journal

OBJECTIVE: To construct 3 expression plasmids for the targeted therapy of thrombosis: single chain variable fragment (scFv) of monoclonal antibody (mAb) 7E3 that can identify and bind platelet glycoprotein GPIIb-IIIa complex, scFv of mAb WAPS12.2 that can identify and bind P selectin (CD62P), a diabody that can identify and bind GPIIb-IIIa and CD62P imultaneously, and to investigate whether the vectors can express correctly. METHODS: This study was carried out at the Laboratory of Hunan Yuantai Biological Technology Co. Ltd, Hubei, China from September 2007 to May 2008. Total RNA of mAb 7E3 cells and WAPS12.2 cells were obtained. Reverse transcriptase …

Eimeria Spp. In Brazilian Water Buffalo, Antonio Carlos F. De Noronha, Jr., Wilma A. Starke-Buzetti, Donald W. Duszynski

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Eimeria species are frequently found in water buffalo (Bubalus bubalis) in Brazil. Here, we report those Eimeria spp. that infect buffalos during their first year of life. Fresh fecal samples were examined from 2 groups (1 group/yr for 2 yr, 2000-2002), each with 18 water buffalo calves (both sexes), from birth through 12 months of age, in Selvíria, MS, Brazil. Five oocyst morphotypes were observed, i.e., Eimeria ellipsoidalis and Eimeria zuernii, both previously described from water buffalo, and 3 other morphotypes consistent with descriptions of known Eimeria spp. from Artiodactyla hosts, but originally described from other …

Environment And Obesity In The National Children's Study, Leonardo Trasande, Christine E. Cronk, Maureen S. Durkin, Marianne E. Weiss, Dale A. Schoeller, Elizabeth A. Gall, Jeanne B. Hewitt, Aaron L. Carrel, Philip J. Landrigan, Matthew W. Gillman

College of Nursing Faculty Research and Publications

Objective: In this review we describe the approach taken by the National Children’s Study (NCS), a 21-year prospective study of 100,000 American children, to understanding the role of environmental factors in the development of obesity.

Data sources and extraction: We review the literature with regard to the two core hypotheses in the NCS that relate to environmental origins of obesity and describe strategies that will be used to test each hypothesis.

Data synthesis: Although it is clear that obesity in an individual results from an imbalance between energy intake and expenditure, control of the obesity epidemic will require understanding of …

Down And Out. The Functional Effects Of Silencing Calsequestrin 1 Or Deleting Both Calsequestrin Genes In Mammalian Muscle, Leandro Royer, Sandrine Pouvreau, Ying Wang, Gerhard Meissner, Jingsong Zhou, Pompeo Volpe, Alessandra Nori, Robert H. Fitts, James L. W. Bain, Feliciano Protasi, Paul D. Allen, Bjorn Knollmann, Danny R. Riley, Eduardo Rios

Biological Sciences Faculty Research and Publications

No abstract provided.

Tree Canopy Types Constrain Plant Distributions In Ponderosa Pine- Gambel Oak Forests, Northern Arizona, Scott R. Abella

Public Policy and Leadership Faculty Research

Trees in many forests affect the soils and plants below their canopies. In current high-density southwestern ponderosa pine (Pinus ponderosa) forests, managers have opportunities to enhance multiple ecosystem values by manipulating tree density, distribution, and canopy cover through tree thinning. I performed a study in northern Arizona ponderosa pine-Gambel oak (Quercus gambelii) forests to measure the influences of tree canopy types on understory plant communities and soil properties. On ten 2.5-acre (1-ha) sites, I sampled five 43-ft2 (4-m2) plots below each of the following five canopy types: openings; single ponderosa pine; and Gambel oak single stems, dispersed clumps, and thickets. …

Osmotic Characteristics And Fertility Of Murine Spermatozoa Collected In Different Solutions, James D. Benson, Si Wei, Hongsheng Men, John K. Critser

Faculty Articles, Papers, and Other Scholarship

Osmotic stress is an important factor that can result in cell damage during cryopreservation. Before ejaculation or collection for cryopreservation, murine spermatozoa are stored in epididymal fluid, a physiologically hyperosmotic environment (w415 mmol/kg). The objectives of this study were to determine the osmotic tolerance limits of sperm motion parameters of ICR and C57BL/6 mouse spermatozoa collected in isosmotic (290 mmol/kg) and hyperosmotic (415 mmol/kg) media, and the effect of the osmolality of sperm collection media on sperm fertility after cryopreservation. Our results indicate that murine spermatozoa collected in media with different osmolalities (290 and 415 mmol/kg Dulbecco’s phosphate buffered saline …

Most Popular Downloads -- January 2009

Institutional Repository Information

January saw 102,754 downloads, from 20,092 available open-access articles.
74% of available articles were downloaded at least once. The average for all articles was 5.11 downloads.

Articles with 150 or more downloads were:

Electronic Rodent Repellent Devices: A Review of Efficacy Test Protocols and Regulatory Actions http://digitalcommons.unl.edu/nwrcrepellants/34 676
Christian Binek: List of Publications http://digitalcommons.unl.edu/physicsbinek/1 526
Arthur Paul Afghanistan Collection Bibliography - Volume II: English and European Languages (2000) http://digitalcommons.unl.edu/afghanenglish/13 463
The Constitutions of the Free-Masons (1734). An Online Electronic Edition. http://digitalcommons.unl.edu/libraryscience/25 419
Role of Esterase gp70 and Its Influence on Growth and Development of Dictyostelium discoideum http://digitalcommons.unl.edu/bioscimicro/77 382
EFFECTS OF …

Breaking The Logjam: Environmental Reform For The New Congress And Administration, David Schoenbrod, Richard B. Stewart, Katrina M. Wyman

Other Publications

No abstract provided.

Gstm1 And Gstt1 Null Polymorphisms And Risk Of Salivary Gland Carcinoma, Sayaka Kondo, Erich M. Sturgis, Fanglin Li, Qingyi Wei, Guojun Li

Faculty, Staff and Student Publications

Glutathione S-transferase (GST) genes detoxify and metabolize carcinogens, including oxygen free radicals which may contribute to salivary gland carcinogenesis. This cancer center-based case-control association study included 166 patients with incident salivary gland carcinoma (SGC) and 511 cancer-free controls. We performed multiplex polymerase chain reaction-based polymorphism genotyping assays for GSTM1 and GSTT1 null genotypes. Odds ratios (ORs) and 95% confidence intervals (CIs) were calculated with multivariable logistic regression analyses adjusted for age, sex, ethnicity, tobacco use, family history of cancer, alcohol use and radiation exposure. In our results, 27.7% of the SGC cases and 20.6% of the controls were null for …

Mutual Information For Testing Gene-Environment Interaction, Xuesen Wu, Li Jiin, Momiao Xiong

Faculty, Staff and Student Publications

Despite current enthusiasm for investigation of gene-gene interactions and gene-environment interactions, the essential issue of how to define and detect gene-environment interactions remains unresolved. In this report, we define gene-environment interactions as a stochastic dependence in the context of the effects of the genetic and environmental risk factors on the cause of phenotypic variation among individuals. We use mutual information that is widely used in communication and complex system analysis to measure gene-environment interactions. We investigate how gene-environment interactions generate the large difference in the information measure of gene-environment interactions between the general population and a diseased population, which motives …

Temperature Influence And Heat Management Requirements Of Microalgae Cultivation In Photobioreactors, Thomas Hagen Mehlitz

Master's Theses

Microalgae are considered one of the most promising feedstocks for biofuel production for the future. The most efficient way to produce vast amounts of algal biomass is the use of closed tubular photobioreactors (PBR). The heat requirement for a given system is a major concern since the best algae growth rates are obtained between 25-30 °C, depending on the specific strain. A procedure to determine temperature influence on algal growth rates was developed for a lab-scale PBR system using the species Chlorella. A maximum growth rate of 1.44 doublings per day at 29 °C (optimal temperature) was determined. In addition, …

Establishment Of A Research Colony Of Marmorkrebs, A Parthenogenetic Crayfish Species, Stephanie A. Jimenez, Zen Faulkes

School of Integrative Biological & Chemical Sciences (Formerly Dept. of Biology)

Marmorkrebs, or marbled crayfish, are recently discovered parthenogenetic crayfish. Its origins are unknown, but it has been introduced into natural ecosystems and could become a highly invasive pest species. Marmorkrebs have potential as a model organism for research in many biological fields because they are genetically uniform. A key element of most successful model organisms is maintaining self-sustaining breeding colonies. We report on our efforts find the best conditions for establishing and maintaining a Marmorkrebs breeding colony for research. The colony was founded by four adults of unknown age (P generation). These foundresses were housed communally in standard aquaria and …

A Question Of Costs: Considering Pressure On White-Collar Criminal Defendants, Sarah Ribstein

Duke Law Journal

Because of the expense of defending white-collar criminal cases, individual corporate defendants can rarely fund their own defenses and often rely on their employers to pay their legal costs. Employers, however, often feel pressure to refuse to pay their employees' attorneys' fees. When employers decline to pay their employees' defense costs, defendants can be, in effect, coerced into pleading guilty because they do not have the financial resources to defend themselves at trial. Commentators have discussed the problem of pressure on white-collar defendants but have not traced the cause of the pressure back to one of its most basic roots: …

The Call To Critique ‘Common Sense’ Understandings About Boys And Masculinity(Ies), Leanne Dalley-Trim

Australian Journal of Teacher Education

This paper is founded upon the premise that ‘common sense’ understandings about boys persist within schools and, given this continuing circulation of such understandings, advocates the need to critique such conceptualising. It does so on the grounds that such understandings, and the essentialist discursive knowledges informing these, fail to take account of the complex and multifarious ways in which boys come to construct themselves as masculine subjects. In demonstrating the short-comings of such ‘common sense’ understandings, and indeed to need to call these into question, the paper examines the ways in which a group of boys took up positions of …

How Cross-Modality Effects During Intraspecific Interactions Of Jumping Spiders Differ Depending On Whether A Female-Choice Or Mutual-Choice Mating System Is Adopted, Fiona R. Cross, Robert R. Jackson

All Peer-Reviewed Publications

An important prediction from game theory is that the value of a resource influences the level to which conflict escalates. Here we use jumping spiders (Salticidae) to consider this prediction in the context of species adopting different mating systems ('female mate-choice' and 'mutual mate-choice'). Our experiments are designed for determining whether the odour of conspecific females, more than the odour of heterospecific females, primes males to escalate conflict with a potential same-sex rival and also whether the odour of conspecific males, more than the odour of heterospecific males, primes females to escalate conflict with a potential same-sex rival. Four species …

Enhanced Estrogen-Induced Proliferation In Obese Rat Endometrium, Qian Zhang, Qi Shen, Joseph Celestino, Michael R Milam, Shannon N Westin, Robin A Lacour, Larissa A Meyer, Gregory L Shipley, Peter J A Davies, Lei Deng, Adrienne S Mccampbell, Russell R Broaddus, Karen H Lu

Faculty, Staff and Student Publications

OBJECTIVE: We tested the hypothesis that the proliferative estrogen effect on the endometrium is enhanced in obese vs lean animals.

STUDY DESIGN: Using Zucker fa/fa obese rats and lean control, we examined endometrial cell proliferation and the expression patterns of certain estrogen-regulated proproliferative and antiproliferative genes after short-term treatment with estradiol.

RESULTS: No significant morphologic/histologic difference was seen between the obese rats and the lean rats. Estrogen-induced proproliferative genes cyclin A and c-Myc messenger RNA expression were significantly higher in the endometrium of obese rats compared with those of the lean control. Expression of the antiproliferative gene p27Kip1 was suppressed …

Swcnt Suppress Inflammatory Mediator Responses In Human Lung Epithelium In Vitro, Eva Herzog, Hugh Byrne, Maria Davoren, Anke-Gabriele Lenz, Albert Duschl, Gertie Janneke Oostingh

Articles

Single walled carbon nanotubes have gained enormous popularity due to a variety of potential applications which will ultimately lead to increased human and environmental exposure to these nanoparticles. This study was carried out in order to evaluate the inflammatory response of immortalised and primary human lung epithelial cells (A549 and NHBE) to single walled carbon nanotube samples (SWCNT). Special focus was placed on the mediating role of lung surfactant on particle toxicity. The toxicity of SWCNT dispersed in cell culture medium was compared to that of nanotubes dispersed in dipalmitoylphosphatidylcholine (DPPC, the main component of lung lining fluid). Exposure was …

Youth Violence Reduction Literature Review, Grand Valley State University, School Of Criminal Justice

Research, Reports, and Publications

No abstract provided.

University Of San Diego Women's Rowing Media Guide 2009, University Of San Diego Athletics Department

Rowing (Women)

24 pages : illustrations, portraits ; 21.5 x 28 cm

University Of San Diego News Print Media Coverage 2009.02, University Of San Diego Office Of Public Affairs

Print Media Coverage (1947-2009)

Printed clippings housed in folders with a table of contents arranged by topic.

Gender Specific Disruptions In Emotion Processing In Younger Adults With Depression, Sara L. Wright, Scott Aaron Langenecker, Patricia J. Deldin, Lisa J. Rapport, Kristy A. Nielson, Allison M. Kade, Lawrence S. Own, Huda Akil, Elizabeth A. Young, Jon-Kar Zubieta

Psychology Faculty Research and Publications

Background: One of the principal theories regarding the biological basis of major depressive disorder (MDD) implicates a dysregulation of emotion-processing circuitry. Gender differences in how emotions are processed and relative experience with emotion processing might help to explain some of the disparities in the prevalence of MDD between women and men. This study sought to explore how gender and depression status relate to emotion processing. Methods: This study employed a 2 (MDD status) × 2 (gender) factorial design to explore differences in classifications of posed facial emotional expressions (N=151). Results: For errors, there was an …

Integrated Cognitive Architectures: A Survey, Hui-Qing Chong, Ah-Hwee Tan, Gee-Wah Ng

Research Collection School Of Computing and Information Systems

This article aims to present an account of the state of the art research in the field of integrated cognitive architectures by providing a review of six cognitive architectures, namely Soar, ACT-R, ICARUS, BDI, the subsumption architecture and CLARION. We conduct a detailed functional comparison by looking at a wide range of cognitive components, including perception, memory, goal representation, planning, problem solving, reasoning, learning, and relevance to neurobiology. In addition, we study the range of benchmarks and applications that these architectures have been applied to. Although no single cognitive architecture has provided a full solution with the level of human …

The Dark Triad: Facilitating A Short-Term Mating Strategy In Men, Peter K. Jonason, Norman P. Li, Gregory D. Webster, David P. Schmitt

Research Collection School of Social Sciences

This survey (N=224) found that characteristics collectively known as the Dark Triad (i.e. narcissism, psychopathy and Machiavellianism) were correlated with various dimensions of short-term mating but not long-term mating. The link between the Dark Triad and short-term mating was stronger for men than for women. The Dark Triad partially mediated the sex difference in short-term mating behaviour. Findings are consistent with a view that the Dark Triad facilitates an exploitative, short-term mating strategy in men. Possible implications, including that Dark Triad traits represent a bundle of individual differences that promote a reproductively adaptive strategy are discussed. Findings are discussed in …

Efficacy And Efficiency In Natural Family Planning Services, Richard J. Fehring

College of Nursing Faculty Research and Publications

Relatively few Catholic couples in the United States use modern methods of natural family planning (NFP). So too, few Catholic physicians and health professionals prescribe the use of NFP methods for their patients. Reasons for low use of NFP methods include their perceived low efficacy; the complexity of learning, using, and teaching these methods; and the prolonged (and often unnecessary) required abstinence. Newer and simplified methods of NFP have been developed by physicians and scientists that are less complex and use modern technologies of detecting fertility and communicating instructions. Catholic physicians and scientists need to continue to answer the call …

Containment Revived: An Alternative Way To Cope With Terrorism, Dr. Andreas M. Bock, Ph.D.

Journal of Strategic Security

"Coercion or negative sanctions are found to have little effect [on terrorism] and, in important instances, are even counterproductive." In other words: fighting terrorism by war is no use, it does not even have a deterrent effect. On the contrary, the employment of massive military power makes it easier for terrorists to justify their attacks, to find broad support, and to recruit new followers. This notwithstanding, I will offer an alternative way to cope with terrorism, using George F. Kennan's concept of containment. This is the link to our effort to effectively fight the terrorist threat: it is a battle …

Spectroscopic Imaging Of Quantum Dot Cellular Interactions, Lorenzo Salford

Masters

Quantum dots (QDs) are a diverse class of engineered nanomaterials that have great potential for use as agents in imaging, diagnostics and drug-delivery because of their intense and photostable fluorescence. Advances in the field of nanotoxicology, however, have recently identified potential risks and hazards associated with exposure to QDs. The main purpose of this research is to investigate the capabilities of a synergistic range of different techniques, including cytotoxicity assays, confocal microscopy and vibrational spectroscopy, to probe their interaction with Biological systems. With the combination of these techniques it is hoped to understand the mechanisms of the interaction of QDs …

Openline Newsletter, February 2009, Civil Service Council

OpenLine Newsletter

A monthly newsletter for Civil Service employees, Volume XXXIII, Number 8, February 2009.

The Use Of A Non-Point Source Pollution Self-Assessment For Greenhouse And Nursery Operators In California, Diane Dejong, Kathleen Delate, Valerie J. Mellano, Karen L. Robb, David A. Shaw

Journal of Extension

Water quality rules adopted in 2001 in San Diego, California, created new requirements for greenhouse and plant nursery growers to manage surface run-off that could potentially affect drinking water, recreational locations, and wildlife habitat. A "Run-off and Non-Point Source Pollution Self-Assessment for Greenhouse and Container Nurseries" was developed as a series of worksheets that translated technical information for growers to meet legal requirements, maintain their property value, and enhance the quality of their environment. Self-assessment results determined a need for additional training on run-off management and prevention pollution through more site-specific fertilization and pest management techniques based on routine monitoring.

Integrated Pest Management And Protection Practices By Limited Resource Farmers, Nii O. Tackie, Louis E.N. Jackai, Ramble Ankumah, Beatrice N. Dingha, Abdul-Wahab Salifu, Oluwagbemiga Ojumu

Journal of Extension

This article focuses on the perceptions of limited resource farmers (LRFs) on integrated pest management (IPM) and protection practices. Data were collected from 90 LRFs in the Alabama Black Belt and analyzed by descriptive statistics. The results revealed that most farmers used pesticides and practiced crop rotation and other practices, yet a majority was not familiar or inadequately familiar with IPM. It is recommended that instituting a coordinated or seamless IPM education program on a long-term or continuing basis will enable the LRFs to be well familiar with and also practice IPM on a sustained basis.

Illinois State Alumni Magazine, February 2009, Illinois State University, University Marketing And Communications

State: The Magazine of Illinois State University (1999-present)

Illinois State University alumni magazine