Digital Commons Network^™

Masculinities And Affective Equality: Love Labour And Care Labour In Men’S Lives, Niall G. Hanlon

Conference papers

No abstract provided.

A Robust Approach To Identifying Tissue-Specific Gene Expression Regulatory Variants Using Personalized Human Induced Pluripotent Stem Cells, Je-Hyuk Lee, In-Hyun Park, Yuan Gao, Jin B. Li, Zhe Li, George Q. Daley, Kun Zhang, George M. Church

Study of Biological Complexity Publications

Normal variation in gene expression due to regulatory polymorphisms is often masked by biological and experimental noise. In addition, some regulatory polymorphisms may become apparent only in specific tissues. We derived human induced pluripotent stem (iPS) cells from adult skin primary fibroblasts and attempted to detect tissue-specific cis-regulatory variants using in vitro cell differentiation. We used padlock probes and high-throughput sequencing for digital RNA allelotyping and measured allele-specific gene expression in primary fibroblasts, lymphoblastoid cells, iPS cells, and their differentiated derivatives. We show that allele-specific expression is both cell type and genotype-dependent, but the majority of detectable allele-specific expression …

Pro Musica Newsletter, Winter 2009, Bowling Green State University. College Of Musical Arts

Pro Musica Newsletters

No abstract provided.

Chaotic Signatures Of Heart Rate Variability And Its Power Spectrum In Health, Aging And Heart Failure, Guo-Qiang Wu, Natalia M. Arzeno, Lin-Lin Shen, Da-Kan Tang, Da-An Zheng, Nai-Qing Zhao, Dwain L. Eckberg, Chi-Sang Poon

VCU Health Publications

A paradox regarding the classic power spectral analysis of heart rate variability (HRV) is whether the characteristic high- (HF) and low-frequency (LF) spectral peaks represent stochastic or chaotic phenomena. Resolution of this fundamental issue is key to unraveling the mechanisms of HRV, which is critical to its proper use as a noninvasive marker for cardiac mortality risk assessment and stratification in congestive heart failure (CHF) and other cardiac dysfunctions. However, conventional techniques of nonlinear time series analysis generally lack sufficient sensitivity, specificity and robustness to discriminate chaos from random noise, much less quantify the chaos level. Here, we apply a …

Virginia Commonwealth University Commencement Program [2009], Virginia Commonwealth University

VCU Commencement Programs

No abstract provided.

Awaken The Sleeping Giant, Edward F. Ansello

Director's Editorials from Age in Action

In order to maintain our commitment to quality, we in the Aging Network need to invest in the future despite the monetary problems of the present. In Virginia, older Virginians are a remarkable resource for our future.

The Older Inmate, Tara Livengood

Case Studies from Age in Action

Educational Objectives

1. Present a general overview of elderly inmates, including statistics, characteristics, crimes, mental and medical illnesses, and disabilities.

2. Describe Virginia’s only geriatric correctional facility.

3. Explain the practice of release planning, focusing especially on geriatric release.

Research Needs In Aquatics, Bruce E. Becker

International Journal of Aquatic Research and Education

No abstract provided.

Biophysiologic Effects Of Warm Water Immersion, Bruce E. Becker, Kasee Hildenbrand, Rebekah K. Whitcomb, James P. Sanders

International Journal of Aquatic Research and Education

No abstract provided.

Apoptotic Engulfment Pathway And Schizophrenia, Xiangning Chen, Cuie Sun, Qi Chen, F. Anthony O'Neill, Dermot Walsh, Ayman H. Fanous, Kodavali V. Chowdari, Vishwajit L. Nimgaonkar, Adrian Scott, Sibylle G. Schwab, Dieter B. Wildenauer, Ronglin Che, Wei Tang, Yongyong Shi, Lin He, Xiong-Jian Luo, Bing Su, Todd L. Edwards, Zhongming Zhao, Kenneth S. Kendler

Human and Molecular Genetics Publications

Background

Apoptosis has been speculated to be involved in schizophrenia. In a previously study, we reported the association of the MEGF10 gene with the disease. In this study, we followed the apoptotic engulfment pathway involving the MEGF10, GULP1, ABCA1 and ABCA7 genes and tested their association with the disease.

Methodology/Principal Findings

Ten, eleven and five SNPs were genotyped in the GULP1, ABCA1 and ABCA7 genes respectively for the ISHDSF and ICCSS samples. In all 3 genes, we observed nominally significant associations. Rs2004888 at GULP1 was significant in both ISHDSF and ICCSS samples (p = 0.0083 and …

Staphylococcus Aureus Immunodominant Surface Antigen B Is A Cell-Surface Associated Nucleic Acid Binding Protein, Nicole M. Mackey-Lawrence, Denise E. Potter, Nuno Cerca, Kimberly K. Jefferson

Microbiology and Immunology Publications

Background

Staphylococcus aureus immunodominant surface antigen B (IsaB) elicits an immune response during septicemia and is generally classified as a virulence factor, but its biological function remains completely undefined. In an attempt to identify staphylococcal RNA-binding proteins, we designed an RNA Affinity Chromatography assay and subsequently isolated IsaB.

Results

Western analysis indicated that IsaB was both secreted and cell-surface associated. Gel Shift analysis confirmed the RNA binding activity but revealed that IsaB bound to any nucleic acid without sequence specificity. IsaB exhibited the highest affinity for double-stranded DNA followed by single-stranded DNA and RNA. Because extracellular DNA has been shown …

Diagnosis Of Infections Caused By Pathogenic Free-Living Amoebae, Bruno Da Rocha-Azevedo, Herbert B. Tanowitz, Francine Marciano-Cabral

Microbiology and Immunology Publications

Naegleria fowleri, Acanthamoeba spp., Balamuthia mandrillaris, and Sappinia sp. are pathogenic free-living amoebae. N. fowleri causes Primary Amoebic Meningoencephalitis, a rapidly fatal disease of the central nervous system, whileAcanthamoeba spp. and B. mandrillaris cause chronic granulomatous encephalitis.Acanthamoeba spp. also can cause cutaneous lesions and Amoebic Keratitis, a sight-threatening infection of the cornea that is associated with contact lens use or corneal trauma. Sappinia pedata has been identified as the cause of a nonlethal case of amoebic encephalitis. In view of the potential health consequences due to infection with these amoebae, rapid diagnosis is critical for early treatment. Microscopic examination and …

Prediction Of Antigenic Epitopes On Protein Surfaces By Consensus Scoring, Shide Liang, Dandan Zheng, Chi Zhang, Martin Zacharias

Radiation Oncology Publications

Background Prediction of antigenic epitopes on protein surfaces is important for vaccine design. Most existing epitope prediction methods focus on protein sequences to predict continuous epitopes linear in sequence. Only a few structure-based epitope prediction algorithms are available and they have not yet shown satisfying performance.

Results We present a new antigen Epitope Prediction method, which uses ConsEnsus Scoring (EPCES) from six different scoring functions - residue epitope propensity, conservation score, side-chain energy score, contact number, surface planarity score, and secondary structure composition. Applied to unbounded antigen structures from an independent test set, EPCES was able to predict antigenic eptitopes …

Monte Carlo Dose Verification Of Prostate Patients Treated With Simultaneous Integrated Boost Intensity Modulated Radiation Therapy, Nesrin Dogan, Ivaylo Mihaylov, Yan Wu, Paul J. Keall, Jeffrey V. Siebers, Michael P. Hagan

Radiation Oncology Publications

Background To evaluate the dosimetric differences between Superposition/Convolution (SC) and Monte Carlo (MC) calculated dose distributions for simultaneous integrated boost (SIB) prostate cancer intensity modulated radiotherapy (IMRT) compared to experimental (film) measurements and the implications for clinical treatments.

Methods Twenty-two prostate patients treated with an in-house SIB-IMRT protocol were selected. SC-based plans used for treatment were re-evaluated with EGS4-based MC calculations for treatment verification. Accuracy was evaluated with-respect-to film-based dosimetry. Comparisons used gamma (γ)-index, distance-to-agreement (DTA), and superimposed dose distributions. The treatment plans were also compared based on dose-volume indices and 3-D γ index for targets and critical structures.

Results …

Talking Bodies: Sensitivity To Desynchronization Of Conversations, Rachel Mcdonnell, Cathy Ennis, Simon Dobbyn, Carol O'Sullivan

Articles

In this article, we investigate human sensitivity to the coordination and timing of conversational body language for virtual characters. First, we captured the full body motions (excluding faces and hands) of three actors conversing about a range of topics, in either a polite (i.e., one person talking at a time) or debate/argument style. Stimuli were then created by applying the motion-captured conversations from the actors to virtual characters. In a 2AFC experiment, participants viewed paired sequences of synchronized and desynchronized conversations and were asked to guess which was the real one. Detection performance was above chance for both conversation styles …

Why Would Anyone Want To Be A Public Interest Lawyer?, Philip G. Schrag

Georgetown Law Faculty Lectures and Appearances

I can barely express how grateful the Law Center is to the Delaneys for their generous gift, or what an honor it is to be designated as the first Delaney Family Professor of Public Interest Law at this incredibly vibrant institution. But our celebration today is not merely the recognition of my scholarship or public service. What the Delaneys have done is to honor not only me but also the hundreds of Georgetown students, faculty, staff and alumni who perform public service. Whether they have achieved high office, like Senators Richard Durbin and Patrick Leahy, or provide free legal services …

A New Triggering Receptor Expressed On Myeloid Cells (Trem) Family Member, Trem-Like 4, Binds To Dead Cells And Is A Dnax Activation Protein 12-Linked Marker For Subsets Of Mouse Macrophages And Dendritic Cells, Hiroaki Hemmi, Juliana Idoyaga, Koji Suda

Publications

Dendritic cells (DCs) are professional APCs that can control immune responses against self and altered self, typically foreign, determinants. DCs can be divided into several subsets, including CD8α⁺ and CD8α^- DCs. These subsets possess specific functions. For example, mouse splenic CD8α⁺, but not CD8α^- DCs selectively take up dying cells and cross-present cell-associated Ags to naive T cells. In this study, we identified genes that were more expressed in CD8α⁺ than CD8α^- DCs by microarray analysis. Only one of these genes, when the extracellular domains were linked to human IgG Fc domain, could …

Insiders' Guide To The Student Academic Conference: 11th Annual Sac, Minnesota State University Moorhead

Student Academic Conference Abstract Books

Minnesota State University Moorhead Student Academic Conference abstract book.

Vol. 84, No. 3 | Winter 2009, Bridgewater College

Bridgewater Magazine

The Ride Stuff

Creating Lakes From Open Pit Mines: Processes And Considerations, Emphasis On Northern Environments, Christopher H. Gammons, Les N. Harris, James M. Castro, Peter A. Cott, Bruce W. Hanna

Geological Engineering

Creating Lakes from Open Pit Mines: Processes and Considerations, Emphasis on Northern Environments. This document summarizes the literature of mining pit lakes (through 2007), with a particular focus on issues that are likely to be of special relevance to the creation and management of pit lakes in northern climates. Pit lakes are simply waterbodies formed by filling the open pit left upon the completion of mining operations with water. Like natural lakes, mining pit lakes display a huge diversity in each of these subject areas. However, pit lakes are young and therefore are typically in a non-equilibrium state with respect …

Activboard Instruction: Does It Increase Reading Skills?, Denita Hall

The Corinthian

The purpose of my research project was to involve students in interactive activities using the ActivBoard in order to guide practices that may lead to increased reading skills. My research project involved an ActivBoard group and a control group. Each group consisted of twenty second grade students in separate classes but taught at the same elementary school. The ActivBoard group engaged in active learning using guided practices incorporating the ActivBoard into the reading curriculum. Each group completed four pre- and posttest reading quizzes, two CRCT reading tests, and two STEEP reading fluency tests. I conducted this study over a two …

“Oh! Dr. Kinsey!”: The Life And Work Of America’S Pioneer Of Sexology, Mikella Procopio

The Corinthian

Alfred Kinsey’s two most famous books Sexual Behavior in the Human Male and Sexual Behavior in the Human Female characterized the sexual behaviors of thousands of Americans. The content of these texts shocked the nation and initiated public discourse on one of the last great taboos in our society. Sex was a controversial topic of discussion in the mid-twentieth century and many researchers had flirted with sex research as it related to hygiene, or as we would refer to it today, the prevention of sexually transmitted infections. But for all the research that was available, no one really knew what …

Examples Of Evidence Of Student Learning, L Suskie

Middle States Information and Publications

EXAMPLES OF EVIDENCE OF STUDENT LEARNING

Biology And Conservation Of Horseshoe Crabs, John T. Tanacredi, Mark L. Bottom, David R. Smith

School of Marine and Environmental Sciences Faculty and Staff Publications and Presentations

We dedicate this book to Drs. Carl N. Shuster, Jr. and Koichi Sekiguchi for their life-long contributions to the biology and conservation of the magnificent horseshoe crab.

Aquilegia, Vol. 33 No. 4, Fall 2009, Newsletter Of The Colorado Native Plant Society, Bob Henry, Ann Henson, Megan Bowes, Jan Loechell Turner, Al Schneider

Aquilegia - Colorado Native Plant Society

No abstract provided.

Aquilegia, Vol. 33 No. 2, Summer 2009, Newsletter Of The Colorado Native Plant Society, Pamela Smith, Loraine Yeatts, Jan Loechell Turner, Thomas Grant, Al Schneider, Katie M. Becklin, Brian Kurzel, Steve Wenger

Aquilegia - Colorado Native Plant Society

No abstract provided.

Characterization And Differentiation Of Peripheral Blood Derived Multipotent Adult Progenitor Cells, Hari Satya Shankar Addagarla

Theses, Dissertations and Capstones

Stem cells are populations of undifferentiated cells that are found in most tissues and act as precursors for regeneration and maintenance. In the future, they could provide promising therapies for diseases which are to date incurable. Our lab developed a novel cell line from the peripheral blood of adult transgenic green fluorescent protein swine and designated them as Peripheral Blood Derived Multipotent Adult Progenitor Cells (PBD-MAPCs). In this study we characterized the mRNA and protein expression profiles of PBD-MAPCs before and after neural differentiation and investigated the potential of PBD-MAPCs to differentiate into myocardial or neural lineages in vitro. We …

Parental Perceptions Of Weight In Their Own Children And In Other Children, Tammy Corbett-Alderman

Theses, Dissertations and Capstones

Childhood obesity rates have risen dramatically in the past 50 years. Interest in the issue of obesity in the United States has grown significantly as our population has become increasingly heavier and our obesity associated health issues have become ever more apparent. Childhood obesity is a persistent problem as 80% of obese children grow up to be obese adults. Despite the alarming increase in childhood obesity little has been done to reverse this epidemic. The purpose of the present study was to determine if parents are able to identify excess weight in children other than their own. It was hypothesized …

Influences Of Writing Project Involvement On The Professional Development Of Teachers, Barbara Dondiego Holmes

Theses, Dissertations and Capstones

Writing is a powerful learning tool that allows students to connect critical thinking across the curriculum. Good writing skills are necessary for students to succeed in higher education and on the job. Teachers, however, are avoiding teaching writing, in part because it has not been included until recently in high stakes testing, and in part because they may not understand how to teach writing and how to grade it. Central West Virginia Writing Project, a site of the National Writing Project, conducts an annual Invitational Summer Institute for teachers of grades K-Adult to teach teachers to teach writing across the …

Determining The Distribution Of Antibiotic Resistant And Fecal Indicator Bacteria In The Ohio River, Caitlin Nicole Swecker

Theses, Dissertations and Capstones

The Ohio River extends 981 miles from Pittsburgh, PA to Cairo, Ill providing drinking water to over three million people, a natural habitat for aquatic life, a public recreation resource, a major transportation route, and a source of water for industry. The Guyandotte River is a highly impacted tributary emptying into the Ohio River in Huntington, WV. The objectives of this study were to determine if sediment load is correlated to the number of antibiotic resistant bacteria (ARB) and determine if a single surface sample is a sufficient representative measurement of ARB populations in a large river. In 2007, subsurface …