Digital Commons Network^™

Regeneration Of Damaged Growth Plate Using Igf-I Plasmid-Releasing Porous Plga Scaffolds, Nirmal Ravi

University of Kentucky Doctoral Dissertations

Growth plate injuries account for 15-30% of long bone fractures in children. About 10% of these result in significant growth disturbances due to formation of a boney bar. If not treated correctly, this can lead to life-lasting consequences of limb length inequalities and angular deformities. Current treatments for growth plate injuries include removal of boney bar and insertion of fat, silicone, bone cement, etc.. This treatment y is inadequate, leaving almost half of these patients with continued deformities. This dissertation reports characterization of a DNA–containing porous poly(lactic-co-glycolic acid) (PLGA) scaffold system, chondrogenesis using insulin-like growth factor I (IGF-I) plasmid-releasing scaffolds …

Investigations Of S-Glutathionylation Of Brain Proteins In The Progression Of Alzheimer's Disease And Of A Potential Glutatione Mimetic As A Treatment Of Alzheimer's Disease, Shelley Faye Newman

University of Kentucky Doctoral Dissertations

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by neurofibrillary tangles, senile plaques and loss of synapses. Many studies support the notion that oxidative stress plays an important role in AD pathogenesis. Previous studies from our laboratory employed redox proteomics to identify oxidatively modified proteins in the AD inferior parietal lobule (IPL). The proteins were consistent with AD pathology and have been central to further investigations of the disease. The present study was focused on the identification of specific targets of protein S-glutathionylation in AD, early AD (EAD), and mild cognative impairment (MCI) using a redox proteomics approach. In AD …

The Culture Connection: Testing A Model Of African American Attitudes Toward Mental Health Service Utilization, Andrea Michelle Smith

University of Kentucky Doctoral Dissertations

The purpose of the current study was to combine the factors previously determined to be related to African American help seeking into a single path model and, using structural equation modeling (SEM), determine the relative influence of each factor in the attitudes toward seeking mental health services (see Figure 2.2) among a community sample of African American adults from several metropolitan areas in the US. As such, SEM was utilized to compare the relative fit of two opposing models within this sample, one where a path from economic barriers to help-seeking attitudes was estimated freely and one where that path …

Applications Of Computational Fluid Dynamics To Planetary Atmospheres, Xiaolong Deng

University of Kentucky Doctoral Dissertations

Computational Fluid Dynamics (CFD) has been applied to many areas. As one of the most important fluids, the atmosphere is closely related to people’s life. Studying the atmospheres on other planets can help people understand the Earth’s atmosphere and the climate and weather phenomena in it. Because of the complexity of a planetary atmosphere and the limitation of observations, applying CFD to the study of planetary atmospheres is becoming more and more popular. This kind of CFD simulations will also help people design the mission to the extra planets.

In this dissertation, through CFD simulations, we studied the three important …

Persistent Currents And Quantum Critical Phenomena In Mesoscopic Physics, Oleksandr Zelyak

University of Kentucky Doctoral Dissertations

In this thesis, we study persistent currents and quantum critical phenomena in the systems of mesoscopic physics. As an introduction in Chapter 1 we familiarize the reader with the area of mesoscopic physics. We explain how mesoscopic systems are different from quantum systems of single atoms and molecules and bulk systems with an Avogadro number of elements. We also describe some important mesoscopic phenomena.

One of the mathematical tools that we extensively use in our studies is Random Matrix Theorty. This theory is not a part of standard physics courses and for educational purposes we provide the basics of Random …

Lipid-Based Paclitaxel And Doxorubicin Nanoparticles To Overcome P-Gp-Mediated Drug Resistance In Solid Tumors, Xiaowei Dong

University of Kentucky Doctoral Dissertations

Multidrug resistance (MDR) is a major obstacle limiting chemotherapeutic efficacy. The purpose of these studies was to investigate the potential application of injectable paclitaxel (PX) and doxorubicin (Dox)-loaded nanoparticles (NPs) engineered from oil-in-water microemulsion precursors for overcoming P-glycoprotein (P-gp)- mediated drug resistance in solid tumors.

An in-vitro study was performed to test whether the oil (stearyl alcohol and cetyl alcohol) used to make lipid nanoparticles could be metabolized. The results showed that the concentrations of the fatty alcohols within nanoparticles, which were quantitatively determined over time by gas chromatography, decreased to only 10-20% of the initial concentration after 15-24 h …

Bootstrap Enhanced N-Dimensional Deformation Of Space With Acoustic Resonance Spectroscopy, David John Link

University of Kentucky Doctoral Dissertations

Acoustic methods can often be used with limited or no sample preparations making them ideal for rapid process analytical technologies (PATs). This dissertation focuses on the possible use of acoustic resonance spectroscopy as a PAT in the pharmaceutical industry. Current good manufacturing processes (cGMP) need new technologies that have the ability to perform quality assurance testing on all products. ARS is a rapid and non destructive method that has been used to perform qualitative studies but has a major drawback when it comes to quantitative studies. Acoustic methods create highly non linear correlations which usually results in high level computations …

Exploring Lesbian And Gay Experiences With Individuals, Systems, And Environments: Patterns Of Response To Heterosexist Prejudice And Discrimination, James Russell Couch

University of Kentucky Doctoral Dissertations

While the general social climate in the U.S. has become more accepting and tolerant of sexual minority individuals, heterosexist discrimination, prejudice and violence continues to affect LGBT individuals, families and communities. While much research literature exists on the experience of minority stress and the psychological consequences of minority stress on sexual minorities, little research has been produced that examines sexual minority coping. Within the last decade, heteronegativity has been suggested as a possible coping response to heterosexism. The goal of the present study was to understand sexual minority responses to heterosexism (including heteronegativity) in a variety of contexts and circumstances. …

Alterations Of Zinc Transporters In Alzheimer's Disease, Ganna Lyubartseva

University of Kentucky Doctoral Dissertations

Alzheimer’s disease (AD), one of the major causes of disability and mortality in Western societies, is a progressive age-related neurodegenerative disorder. Increasing evidence suggests the etiology of AD may involve disruptions of zinc (Zn) homeostasis. We hypothesize that disruption of Zn homeostasis leads to alterations of Zn transporter (ZnT) proteins, resulting in increased production of neurotoxic amyloid beta (Aβ) peptide in AD brain. To address this hypothesis we carried out the following studies.

1. We characterized alterations of ZnT-1, ZnT-4 and ZnT-6 in the brain of preclinical AD (PCAD) subjects, who show no overt clinical manifestations of AD but demonstrate …

Examining The Construct Of Perfectionism: A Factor-Analytic Study, Agnes Mariann Stairs

University of Kentucky Doctoral Dissertations

The construct of perfectionism is related to many important outcome variables. However, the term “perfectionism” has been defined in many different ways, and items comprising the different existing scales appear to be very different in content. The overarching aim of the present set of studies was to help clarify the specific unidimensional constructs underlying what is called “perfectionism”. First, trained raters reliably sorted items from existing measures of perfectionism into nine dimensions. An exploratory factor analysis, followed by a confirmatory factor analysis on an independent sample, resulted in a 9 scale, 61 item measure, called the Measure of Constructs Underlying …

Analysis Of Security Measures For Sequences, Ramakanth Kavuluru

University of Kentucky Doctoral Dissertations

Stream ciphers are private key cryptosystems used for security in communication and data transmission systems. Because they are used to encrypt streams of data, it is necessary for stream ciphers to use primitives that are easy to implement and fast to operate. LFSRs and the recently invented FCSRs are two such primitives, which give rise to certain security measures for the cryptographic strength of sequences, which we refer to as complexity measures henceforth following the convention. The linear (resp. N-adic) complexity of a sequence is the length of the shortest LFSR (resp. FCSR) that can generate the sequence. Due …

Paleogeographic Reconstructuion Of The St. Lawrence Promontory, Western Newfoundland, John Stefan Allen

University of Kentucky Doctoral Dissertations

Neoproterozoic-Early Cambrian continental rifting related to the breakup of the supercontinent Rodinia framed the continental margin of eastern Laurentia and the departing cratons around the opening Iapetus Ocean. The result of continental extension was the production of a zig-zag set of promontories and embayments on the eastern Laurentian margin defined by northeast-trending rift segments offset by northwesttrending transform faults.

The St. Lawrence promontory defines the Laurentian margin in western Newfoundland. There, Neoproterozoic-Carboniferous clastic, volcanic, and carbonate successions record protracted continental rifting and passive-margin thermal subsidence followed by destruction of the margin during the early, middle, and late Paleozoic Appalachian orogenic …

Experimental And Molecular Dynamics Simulation Studies Of Partitioning And Transport Across Lipid Bilayer Membranes, Ravindra Wadhumal Tejwani

University of Kentucky Doctoral Dissertations

Most drugs undergo passive transport during absorption and distribution in the body. It is desirable to predict passive permeation of future drug candidates in order to increase the productivity of the drug discovery process. Unlike drug-receptor interactions, there is no receptor map for passive permeability because the process of transport across the lipid bilayer involves multiple mechanisms. This work intends to increase the understanding of permeation of drug-like molecules through lipid bilayers.

Drug molecules in solution typically form various species due to ionization, complexation, etc. Therefore, species specific properties must be obtained to bridge the experiment and simulations. Due to …

Using Thr Ffm To Understand And Integrate The Deficits Of Psychopathy, Karen J. Derefinko

University of Kentucky Doctoral Dissertations

Psychopathy is associated with several behavioral and psychophysiological deficits. Lynam (2002) has argued that the use of an overarching conceptualization of psychopathy can provide a parsimonious explanation of psychopathic pathology. The current study examined relations between tasks used to explore psychopathic pathology and dimensions from the Five Factor Model of personality. Undergraduate participants completed the NEO PI-R, the BART, a go/no-go task, an emotional morph task, and provided physiological responses to stimuli. While hypothesized relations to FFM psychopathy composites were generally unsupported, other interesting relations to traits were identified. Results indicated that hypoarousal to negative stimuli was negatively related to …

Viable Institutions, Judicial Power, And Post-Communist Constitutional Courts, Kirill Mikhaylovich Bumin

University of Kentucky Doctoral Dissertations

In pursuing their goals, newly-created constitutional courts of Eastern Europe and the former Soviet republics are affected by their institutional setting and capabilities. Yet, previous studies did not explore how constitutional courts develop over time and what noteworthy implications for politics and society result from their institutional growth. To address this gap in the literature, I measured a variety of organizational characteristics and constructed an index of institutional development for the twenty eight constitutional courts in the post-communist countries from the initial year of their transitions through 2005. I argued that high values on this measure (which I labeled the …

Aspects Of The Geometry Of Metrical Connections, Matthew J. Wells

University of Kentucky Doctoral Dissertations

Differential geometry is about space (a manifold) and a geometric structure on that space. In Riemann’s lecture (see [17]), he stated that “Thus arises the problem, to discover the matters of fact from which the measure-relations of space may be determined...”. It is key then to understand how manifolds differ from one another geometrically. The results of this dissertation concern how the geometry of a manifold changes when we alter metrical connections. We investigate how diverse geodesics are in different metrical connections. From this, we investigate a new class of metrical connections which are dependent on the class of smooth …

Imide-Functionalized Conjugated Polymers: Synthesis, Structure-Property And Device Studies, Xugang Guo

University of Kentucky Doctoral Dissertations

Organic semiconductors are widely studied as potential active components for consumer electronics due largely to their easily tuned properties and the promise of lower-cost solution-based processing technology. Imide-functionalized organic small molecule compounds have been one of the more important and studied organic semiconductors. However, very few imide-functionalized conjugated polymers have been reported in the literature. The body of this dissertation focuses on the synthesis, structure-property and device studies of imide-functionalized conjugated polymers. Reasons for choosing arylene imides as polymer building blocks include: a) they impart low-lying LUMOs to polymers, allowing band-gap engineering through choice of comonomers with variable electron-donating ability; …

Investigation Of Surface Fine Grained Laminae, Streambed, And Streambank Processes Using A Watershed Scale Hydrologic And Sediment Transport Model, Joseph Paul Russo

University of Kentucky Doctoral Dissertations

Sediment transport at the watershed scale in the Bluegrass Region of Kentucky is dominated by surface fine grained laminae, streambed, and streambank erosion; high instream sediment storage; and surface erosion processes. All these processes can be impacted by agricultural, urban, and suburban land-uses as well as hydrologic forcing. Understanding sediment transport processes at the watershed scale is a need for budgeting and controlling sediment pollution, and watershed modeling enables investigation of the cumulative effect of sediment processes and the parameters controlling these processes upon the entire sediment budget for a watershed. Sediment transport is being modeled by coupling the hydrologic …

Pattern Recognition Integrated Sensing Methodologies (Prisms) In Pharmaceutical Process Validation, Remote Sensing And Astrobiology, Thaddaeus S. Hannel

University of Kentucky Doctoral Dissertations

Modern analytical instrumentation is capable of creating enormous and complex volumes of data. Analysis of large data volumes are complicated by lengthy analysis time and high computational demand. Incorporating real-time analysis methods that are computationally efficient are desirable for modern analytical methods to be fully utilized. The use of modern instrumentation in on-line pharmaceutical process validation, remote sensing, and astrobiology applications requires real-time analysis methods that are computationally efficient.

Integrated sensing and processing (ISP) is a method for minimizing the data burden and sensing time of a system. ISP is accomplished through implementation of chemometric calculations in the physics of …

Long-Period Ground Motions In The Upper Mississippi Embayment From Finite-Fault, Finite-Difference Simulations, Kenneth A. Macpherson

University of Kentucky Doctoral Dissertations

A 3D velocity model and 3D wave propagation code have been employed to simulate long-period ground motions in the upper Mississippi embayment. This region is exposed to seismic hazard in the form of large earthquakes in the New Madrid seismic zone and observational data are sparse, making simulation a valuable tool for predicting the effects of large events. These simulations were undertaken in order to estimate ground-motion characteristics and to investigate the influence of the 3D embayment structure and finite-fault mechanics. There are three primary fault zones in the New Madrid seismic zone, each of which was likely associated with …

The Interplay Between The Expression And Functions Of Wnt13 Isoforms During Apoptosis In Bovine Aortic Endothelial Cells, Tao Tang

University of Kentucky Doctoral Dissertations

Wnt proteins are crucial for development/homeostasis by controlling cell fate including apoptosis (Moon RT et al. 1997). Three humanWnt13 isoforms were identified: the secreted Wnt13A, mitochondrial Wnt13B, and nuclear Wnt13C forms; and nuclear Wnt13 had an increased sensitivity to LPS/TNF-induced apoptosis in primary endothelial cells (EC); both Wnt13B and C mRNA contain two start codons (AUG+1 and +74), but the same protein encoded from AUG+74 by Wnt13C was expressed lower than Wnt13B (Struewing IT et al.2006). We hypothesize that during EC apoptosis, the nuclear Wnt13C expression is regulated translationally; nuclear Wnt13 favors apoptosis through regulating the activity/expression of apoptosis-related factors; …

Study Of Qcd Critical Point Using Canonical Ensemble Method, Anyi Li

University of Kentucky Doctoral Dissertations

QCD at non-zero baryon density is expected to have a critical point where the finite temperature crossover at zero density turns into a first order phase transition. To identify this point, we use the canonical ensemble approach to scan the temperaturedensity plane through lattice QCD simulations with Wilson-type fermions. In order to scan a wide range of the phase diagram, we develop an algorithm, the ”winding number expansion method” (WNEM) to fix the numerical instability problem due to the discrete Fourier transform for calculating the projected determinant. For a given temperature, we measure the chemical potential as a function of …

Three Essays On Residential Real Estate Brokerage, Jason S. Beck

University of Kentucky Doctoral Dissertations

The first essay investigates how individual characteristics influence sales outcomes for houses they help transact. It develops hedonic housing models to assess the impacts of agent characteristics such as the level of recent and concurrent agent activity on sales outcomes (price and time on market). This is done in a Multiple Listing Service (MLS) setting using seven years of data obtained from a large Midwestern city. I find evidence that more active listing agents sell homes more quickly, though they do so to the detriment of final sales price. I also find that more listings concurrently held by agents have …

Analysis Of Progessive Lag Reinforcement Schedules And Environmental Cues On Verbal Response Variability, Brian Esteve

LSU Doctoral Dissertations

This study examined the effectiveness of progressive lag reinforcement schedules to increase novel and appropriate verbal responses to academic skills for three children with language delays. The effect of visual cues on verbal responses was evaluated in conjunction with progressive lag reinforcement schedules using a multiple baseline with an embedded alternating treatments design. Basic research utilizing lag reinforcement schedules have shown to increase variable behavior in both animal and human participants; however, little research has been conducted to evaluate lag reinforcement schedules in applied settings. The results of this study preliminarily indicate that progressive lag reinforcement schedules are an effective …

In My Humble Opinion: Testing The Sprial Of Silence In Computer-Mediated And Face-To-Face Contexts, Robert James Zuercher

University of Kentucky Master's Theses

The purpose of this investigation is to further an understanding of the spiral of silence theory as it functions within both face-to-face (FtF) and computer-mediated contexts. Computer-mediated communication (CMC is often touted for being an empowering medium as it affords its users anonymity. This finding could have an impact on whether the spiral of silence occurs within CMC. Previous studies have relied upon hypothetical scenarios and have established weak support for the theory. Despite this study’s utilization of a within-subjects experimental design, however, no significant differences in minority opinion holders’ fear of isolation were found. Similarly, no significant relationship was …

The Law V. The Stranger: Language Interpretation And Legal Space In Lexington, Ky, Karen S. Kinslow

University of Kentucky Master's Theses

This thesis examines the role of interpretation in legal encounter in Lexington, Kentucky. Through an analysis of legal and interpretation practices, this study seeks to ascertain how these practices may affect non-native or low-proficiency English speakers’ (LLPs) experiences with both federal and local laws and legal spaces. This place-based study involves in-depth qualitative research. Using the methodological framework of feminist geo-jurisprudence, this research contributes to our understanding of 1) the limits of the publicity of legal space and, more specifically, the ways in which language barriers can prevent legal inclusion; 2) local strategies and tactics for dealing with the challenges …

Electrical And Mechanical Properties Of Mwcnt Filled Conductive Adhesives On Lead Free Surface Finished Pcb's., Keerthi Varma Mantena

University of Kentucky Master's Theses

Electrically conductive adhesives (ECA) are an alternative to tin/lead solders for attaching Surface Mount Devices (SMD) in electronic assemblies. ECAs are mixtures of a polymer binder (for adhesion) and conductive filler (for electrical conductivity). They bring more conductivity, higher strength, less weight and longer durability than metal alloys. ECAs can offer numerous advantages such as fewer processing steps, lower processing temperature and fine pitch capability. Multi walled carbon nanotubes (MWCNT) were used as conductive fillers in this research because of their novel electronic and mechanical properties.

The high aspect ratio of the nanotubes makes it possible to percolate at low …

Bedrock Mapping Of The Winchendon (1:25,000) Quadrangle (Ma-Nh): Evidence For Discontinous Deformation Along The Bronson Hill-Central Maine Boundary Zone, Timothy M. O'Brien

University of Kentucky Master's Theses

Bedrock geology of the Winchendon quadrangle is divided into two zones based on structures and lithology. The eastern zone consists of tightly folded Silurian Rangeley and Paxton metasediments and pegmatites. Planar, NNE-SSW striking, W dipping foliations are characterized by alternating phyllosilicate-rich and leucosome layers. Fold axes of tight to isoclinal upright and recumbent folding are parallel to SSW-NNE shallowly plunging sillimanite, quartz and muscovite lineations. In the western zone Rangeley schists were intruded by Devonian Hardwick and Coys Hill and Fitzwilliam plutons. Planar NNE-SSW striking foliations dip shallowly to steeply west. Fold axes of tight-to-isoclinal asymmetric to recumbent folds plunge …

Variation In C/P Ratios In Devonian-Mississippian Marine Shales: Testing The Productivity-Anoxia Feedback Model, Brian T. Scott

University of Kentucky Master's Theses

Carbon/phosphorus ratios for late Devonian-early Mississippian marine black shales along a transect from the Illinois Basin, across the Cumberland Saddle, and into the Appalachian Basin were evaluated to assess the role of productivity in organic carbon accumulation. Phosphorus is a key limiting nutrient for biological productivity in marine environments and may be regenerated preferentially relative to organic carbon, the amount of regeneration possibly being related to bottom-water anoxia. A positive feed-back mechanism (more specifically, productivity-anoxia feedback or PAF) has been proposed between water-column anoxia, high benthic regeneration of phosphorus, and marine productivity. This regeneration of phosphorus under anoxic conditions and …

Use Of Surface Geophysical Techniques To Locate A Karst Conduit In The Cane Run - Royal Spring Basin, Kentucky, Ganesh N. Tripathi

University of Kentucky Master's Theses

Groundwater flow in karst terrains is difficult to map because it can be concentrated through conduits that do not necessarily coincide with the surface features. We applied electrical resistivity (ER) and self-potential (SP) techniques at three sites to locate an inferred trunk conduit feeding a major spring in the Inner Bluegrass region of Kentucky. Royal Spring is the primary water supply for the city of Georgetown; the upper part of its basin coincides with the Cane Run watershed. ER and SP profiles were perpendicular to the inferred trunk conduit orientation. ER profiles (972 m total length) were measured using a …