Digital Commons Network^™

Characterizing The Role Of Primary Cilia In The Hair Follicle And Skin, Jonathan Merle Lehman

All ETDs from UAB

Primary cilia have been established as a nearly ubiquitous microtubule based signaling organelle, yet their function in many organs, including the hair and skin, is unknown. We hypothesized that the primary cilium would play vital roles in the hair follicle and skin based on the cilium's well characterized interactions with the Shh and Wnt developmental signaling pathways. This dissertation describes our efforts to determine the localization of the primary cilia in the murine hair follicle and assess the function of this organelle in the hair and skin. We used confocal immunofluorescence microscopy to virtually section the entire hair follicle and …

Analysis Of The Malaria Vaccine Potential Of Plasmodium Falciparum Merozoite Surface Protein-3, Stephen James Jordan

All ETDs from UAB

Malaria causes an estimated 1-3 million deaths each year, with the majority of deaths being a result of infection with Plasmodium falciparum. No commercially available vaccine currently exists, but multiple subunit-based vaccines are in development including one based on the promising vaccine candidate P. falciparum Merozoite Surface Protein-3 (PfMSP3). PfMSP3 varies in sequence between P. falciparum isolates, but all variation is restricted to the N-terminal domain. This observation has lead to the development of a PfMSP3-based vaccine comprised solely of the highly conserved, yet poorly immunogenic, C-terminal domain. The rationale for supporting the C-terminal domain relies entirely on its conserved …

Polysaccharide Specific B Cells: A Study Of Their Development And Function, Jeremy B. Foote

All ETDs from UAB

Polysaccharides are important structural components of bacterial capsule and cell walls. Polysaccharide-specific antibodies are an important component of serologic memory capable of protecting against infection by pathogenic microorganisms. An understanding of the biology and function of polysaccharide specific B cells is necessary for formulation of vaccines designed to induce polysaccharide specific memory and long-lived antibody production. The goal of this dissertation was to examine the development of naïve DEX-specific B cells and examine their capacity to generate memory and long-lived plasma cells in response to polysaccharide α 1→3-dextran (DEX), which is expressed by the opportunistic pathogens Enterobacter cloacae, Histoplasma capsulatum …

Mitochondrial Bioenergetics And Cellular Stress, Brian Dranka

All ETDs from UAB

Mitochondria are responsible for most of the energy produced in human tissues, and this is dependent on the reduction of oxygen (O2) to water by the mitochondrial respiratory chain. Defects in mitochondrial energy production are now recognized to be involved in diabetes, cancer, cardiovascular disease, and other pathologies. To date, studies of these defects have employed quantification of O2 consumption in isolated, purified mitochondria. By using this strategy however, the cellular context, role of glycolysis, and normal regulation of mitochondrial function by metabolite availability are lost. Thus, an understanding of how mitochondria function and respond to stimuli in an intact …

Role Of Syndecan-1 As Key Regulator Of Multiple Myeloma Pathogenesis, Yekaterina Borisovna Khotskaya

All ETDs from UAB

Syndecan-1 (CD138), a transmembrane heparan sulfate-bearing proteoglycan, is expressed at high levels on most myeloma cells and is shed into the microenvironment. In patients, high levels of serum syndecan-1 are indicative of poor prognosis and elevation of shed syndecan-1 in animal models dramatically enhances tumor growth, angiogenesis and metastasis. Because syndecan-1 is a key regulator of myeloma pathogenesis, we hypothesized that reduction of syndecan-1 levels expressed by the myeloma cells will block their growth and dissemination. Syndecan-1 knockout and knockdown variants of two human myeloma cell lines, CAG and RPMI-8226, were developed using short hairpin RNA (shRNA) technology. In vitro, …

Impact Of Genetic And Epigenetic Variability In Response To Two Test Drugs 5-Fluorouracil And Lansoprazole, Adam Michael Lee

All ETDs from UAB

Pharmacogenetics has proven to be an invaluable tool in predicting variability in drug response; however, there are numerous incidences where genetics cannot fully explain interindividual drug variability. Other factors, such as the epigenetic mechanism of DNA methylation, may offer an alternate explanation. Therefore, this dissertation focuses on both genetic and epigenetic regulation in order to gain a better understanding of the molecular basis behind drug response variability. Initial investigations focused on the antineoplastic agent 5-Fluorouracil (5-FU), which can produce severe toxicity in patients with deficiency in dihydropyrimidine dehydrogenase (DPD). Genotypic studies have identified >32 sequence variants in the DPYD gene; …

Oxidative Stress-Stimulated Vascular Calcification, Chang Hyun Byon

All ETDs from UAB

Oxidative stress plays a critical role in pathogenesis of atherosclerosis. However, the effect of oxidative stress-induced molecular signaling in development of vascular calcification, a feature of advanced atherosclerosis, has not been defined. Osteogenic differentiation of vascular smooth muscle cells (VSMC) is critical in development of vascular calcification. We determined hydrogen peroxide (H2O2)-induced oxidative stress promoted a phenotypic switch of mouse primary VSMC from contractile to osteogenic phenotype. This effect was associated with increased expression and transactivity of Runx2, a key transcription factor for osteogenic differentiation. Inhibition of Runx2 using short hairpin RNA blocked oxidative stress-induced VSMC calcification. By contrast, adenovirus-mediated …

Psychosocial And Affective Mediators Of Ethnic Group Differences In Stressor-Evoked Changes In Systolic Blood Pressure And Pain Perception, Renata Okonkwo

All ETDs from UAB

African Americans, compared to non-Hispanic whites, report greater levels of clinical pain and experience more pain-related disability (AmericanHeartAssociation, 2005; C. L.Edwards, Fillingim, & Keefe, 2001; R. R. Edwards, Doleys, Fillingim, & Lowery, 2001; McCracken, Matthews, Tang, & Cuba, 2001; J. L. Riley et al., 2002). Furthermore, African Americans consistently differ from primarily non-Hispanic white samples on experimentally induced pain. We examined ethnic group differences in the effects of exposure to a brief stressor on individuals' pain responses and changes in SBP and heart rate (HR). In addition, we examined the mediational role of perceived racial discrimination, and differences in the …

Intracellular Trafficking Of The Hantaviral Nucleocapsid Protein And Its Function In Modulation Of Immune Signaling, Steven Joe Ontiveros

All ETDs from UAB

Old World and New World hantaviruses, family Bunyaviridae, mature intracellularly within cellular compartments. Although it is generally accepted they assemble and bud in the Golgi apparatus the site remains controversial for New World hantaviruses, because some studies have raised the possibility of their maturation at the plasma membrane. Furthermore, the site of assembly hantaviruses still remains undetermined. The nucleocapsid (N) protein has been proposed to play a key role in facilitating assembly. To gain insight into the assembly pathways of Old World hantaviruses, we examine the intracellular trafficking of the Hantaan (HTN) virus N protein. We show progressive redistribution of …

Involvement Of Reactive Oxygen Species In The Acute Development Of Insulin Resistance Following Trauma And Hemorrhage, Lidong Zhai

All ETDs from UAB

Hyperglycemia develops in the intensive care unit in many patients following injury, infection and critical illness. However, little is known about the mechanism of acute development of insulin resistance that causes this hyperglycemia, even though reducing or normalizing the elevated glucose levels has become a major therapeutic target in many ICUs. Using rodent models of injury, acute hepatic insulin resistance occurs within minutes and continues for at least several hours even following fluid resuscitation. We have previously reported that TNF-alpha; is necessary for the hepatic insulin resistance in the rat following resuscitation. However, whether TNF-alpha; plays a role in the …

The Effects Of Bisphenol A (Bpa) On Erbb2-Positive Breast Cancer, Sarah Jenkins

All ETDs from UAB

Bisphenol A (BPA) is a synthetically made compound used to produce a myriad of consumer goods. Recent studies have shown BPA to leach from these products in appreciable amounts, resulting in nearly ubiquitous exposure. In this study, we assessed whether chronic administration to a range of low concentrations of BPA could accelerate spontaneously developing mammary cancer using a transgenic model that over-expresses wild type ErbB2/neu transgene (MMTV-erbB2). MMTV-erbB2 mice were provided drinking water containing 0 (control), 2.5 (BPA 2.5), 25 (BPA 25), 250 (BPA 250), or 2500 (BPA 2500) µg BPA/L from eight weeks of age until sacrifice. This range …

The Role Of Vea On Aspergillus Flavus Infection Of Peanuts, Corn And Cotton, Ana M. Calvo, R.M. Duran, J.W. Cary

Faculty Articles, Papers, and Other Scholarship

The aflatoxin-producing fungus Aspergillus flavus is a causal agent of preharvest contamination of food commodities such as oil seed crops worldwide. Peanut, corn and cottonseed are among the oil seeds that are susceptible to aflatoxin contamination during invasion of these crops by A. flavus. Contamination of agricultural commodities with aflatoxins can result in serious economic hardships to producers and adverse health impacts in both humans and domestic animals. It is therefore of great importance to determine ways to control A. flavus dissemination, survival and toxin formation. In this study we demonstrate the role of the veA regulatory gene in the …

Expression Of Sox 11 During Tooth Formation, Reem Atout

All ETDs from UAB

No abstract provided.

The Effect Of Stearic Acid On Breast Cancer Development And Progression, Lynda Michelle Evans

All ETDs from UAB

earate is an 18-carbon saturated fatty acid that is found in many foods in the western diet including beef and chocolate. Cell culture studies indicate stearate may have various anti-cancer properties including inhibition of cancer cell proliferation and invasion, morphological changes, and induction of apoptosis. Animal studies have found that dietary stearate delays tumor development and decreases tumor incidence. To date, many of the mechanisms underlying these processes are unclear. In this study, evidence is presented showing stearate induces morphological changes in breast cancer cells. Inhibition of de novo diacylglycerol (DAG) generation and subsequent protein kinase C (PKC) activation inhibits …

The Protective Role Of Transglutaminase 2 In Ischemic Stroke, Anthony J. Filiano

All ETDs from UAB

Stroke is a leading cause of long term disabilities in the US. Currently, administration of thrombolytics is the only approved therapy. Due to the variability, small management time window, and lack of options for effective treatment, there is a clear need for new compounds to alleviate cell death post-stroke. Transglutaminase 2 (TG2) can decrease apoptotic signaling during stroke and lead to increased neuronal survival making it a potential target for therapeutic intervention. TG2, a multifunction enzyme that has both transamidase (TG) and GTPase activities, amongst others, has recently been shown to be upregulated in numerous neurodegenerative conditions, including stroke. In …

Form And Function Of Expressive Morphology: A Case Study Of Russian, Olga Steriopolo

Russian Language Journal

In this paper, I conduct a detailed case study of expressive suffixes in Russian. Although the suffixes under investigation have the same function (expressive), they differ significantly in their formal properties. I identify two major semantic types of expressive suffixes: attitude suffixes, which convey the speaker’s attitude toward the referent, and size suffixes, which both convey the speaker’s attitude and refer to the size of the referent. I argue that the two different semantic types map onto different syntactic types. Attitude suffixes are syntactic heads, while size suffixes are syntactic modifiers. As heads, attitude suffixes determine the formal properties (syntactic …

Birth Language Attrition And Reacquisition In Russian Adoptees, Avram J. Lyon

Russian Language Journal

The wave of adoption from Russia and other countries of the former Soviet Union has brought a large number of incomplete Russian speakers into the United States; over 71,000 orphans from Russia, Ukraine, Kazakhstan, and Belarus have been issued United States entry visas since 1992 (U.S. Department of State 2007 and 2009; Ruggiero 2007). This is part of a larger trend toward international adoption, amounting to several hundred thousand children in the past decades. Children adopted by American families experience a radical reduction in birth language exposure and must quickly acclimate to and become proficient in the language of the …

Volume 2, Issue 8, September 2009, Parkland College

The Toilet Paper

Come check out the animated movie Persepolis; Need a good video for a class or project?; TRiO/Student Support Services offers FREE services and resources for eligible students; Lions and Tigers and Swine Flu, Oh My

Strength In Numbers: Setting Quantitative Criteria For Listing Species Under The Endangered Species Act, Kalyani Robbins

Akron Law Faculty Publications

My primary thesis is that the Fish & Wildlife Service and the National Marine Fisheries Service need to set quantitative criteria for listing species under the Endangered Species Act in order to promote consistency, transparency, and efficiency. I suggest a model for doing so, the use of which would create an opportunity to move beyond the political quagmire surrounding the selection of vulnerable species for preservation. Like my other environmental scholarship, the article merges scientific research in the field of conservation biology with legal analysis. With the status quo, listing decisions often turn on wildly different factors, including some not …

Infinite Hope-- Introduction To The Symposium: The 140th Anniversary Of The Fourteenth Amendment, Elizabeth Reilly

Akron Law Faculty Publications

The Fourteenth Amendment embodies hope. This article introduces the Symposium celebrating the 140th anniversary of its ratification, held at the University of Akron. The symposium was a fruitful occasion to reflect upon the meaning of the Amendment to its Framers in Congress and as it was initially interpreted by the United States Supreme Court and the public, and to examine the lasting impacts of both conceptions. Our participants especially examined three of the Supreme Court's earliest forays into applying the Fourteenth Amendment: The Slaughter House Cases, Bradwell v. Illinois, and Cruikshank v. United States. Those forays succeeded in cramping the …

Impact Of Alcohol On Dietary Behaviors Of Male College Students, Cheryl Anne Rozela

Masters Theses

Research has suggested that many male students increase consumption of alcohol during their college years (Von Ah, Ebert, Ngamvitroj, Park & Kang, 2001). As alcohol consumption increases during this time many students also make less healthy dietary choices, suggesting a relationship between alcohol use and dietary patterns may be present (Adams & Rini, 2007). Although suggestive of a relationship, there is a lack of research documenting an association between alcohol use and dietary behaviors of male college students, therefore, the current study utilized a convenience sample of twenty male participants from a midwestem university and a series of five open-ended …

A Content Analysis Of Food Advertisements During Children's Tv Programming, Jillian Hurt

Masters Theses

The purpose was to determine nutritional quality of foods and marketing techniques and messages used in food ads during children's television programming. Programming was taped on 4 networks (PBS, Disney, Nickelodeon and Cartoon Network) from 8 a.m. to 5 p.m. for l day each, from July 8 to July 11, 2008.

One third of ads shown in 36 hours of programming were for food. Eighty-four percent of food ads promoted foods that were high in fat, saturated fat, added sugar and sodium or low in vitamins, minerals, fruits, vegetables and fiber. Animation was used in 44% and taste in 58% …

Perspective-Taking In Adolescents With Asperger Syndrome And Nonverbal Learning Disorder, Caitlin Scaliatine

Masters Theses

Theory of mind is the ability to attribute mental states to oneself and other individuals, allowing us to predict the thoughts and feelings of others based on their behaviors. Research has established that individuals with Asperger syndrome (AS) experience difficulty with advanced theory of mind tasks; however, theory of mind abilities have not been evaluated for individuals with nonverbal learning disability (NVLD). The following test battery was administered to four adolescents with AS, five adolescents with NVLD, and five adolescents with neurotypical development to examine their theory of mind abilities: the Pragmatic Judgment and lriferences subtests from the Comprehensive Assessment …

Dynamics Of Aggregation Formation In Japanese Beetles, Popillia Japonica, Katelyn A. Kowles

Masters Theses

For most insect aggregations to form, they need to be started by an initial individual (the pioneer) andjoined by later individuals (the joiners). Pioneers and joiners may differ with regard to characteristics such as sex and body size. We carried out three field experiments to examine the characteristics of Japanese beetles, Popillia japonica, pioneering and joining aggregations on host plants. Individual beetles were captured as they arrived on uninhabited grape plants, as well as plants designed to simulate aggregations with model beetles and feeding damage. For all experiments and treatments, the beetles arriving were significantly female-biased, with pioneer females having …

Ecology And Impacts Of Lianas In Regenerating Forests, Laura Marie Ladwig

Masters Theses

Although they are important components of forest communities, the general ecology of temperate lianas, especially during forest regeneration, is largely unknown. The dependence of lianas on other plants for physical support makes them a potentially important driver of community dynamics. I examined current and past vegetation data from an old-field succession study in the Piedmont region of New Jersey, USA to determine the dynamics of liana expansion and their role in forest regeneration. Fifty years of vegetation data were utilized to determine community controls and population dynamics oflianas during expansion. Five lianas, Celastrus orbiculatus, Lonicera japonica, Parthenocissus quinquefolia, Toxicodendron radicans, …

Aging In The Mammalian Olfactory System, Stephen Clark

Masters Theses

The purpose of this investigation was to investigate genetic and age~related differences in the mammalian olfactory system. The molecule of interest is apolipoprotein E (apoE), a component oflipoprotein molecules which functions in the transport of cell membrane components (triglycerides, lipids, and cholesterol). Specifically in the nervous system, apoE is synthesized by glial cells that function in recycling dead nervous tissue components for delivery to growing or dividing neurons. ApoE is present in three protein isofon11s: apoE2, which has been associated with type III hyperlipoproteinemia; apoE3, rhe normal human isoforrn; and apoE4, which has been associated with Alzheimer's disease (AD). The …

Interaction Of Apoe With Estrogen In The Olfactory System During Nerve Maintenance And Recovery, Michael M. Tonsor Ii

Masters Theses

Estrogen replacement profoundly aids in nerve regeneration after injury in adult mammalian models. Estrogen replacement also protects against olfactory dysfunction in mammals. The mechanism underly1ng estrogen's neuroprotective effects remains ambiguous. My goal is to investigate and better clarify if apolipoprotein E (apoE ), a lipid transporting protein, is a critical intermediary to estrogen's effects on nerve maintenance and regeneration.

My hypothesis is that apoE is a mediator of estrogen's beneficial effects. This hypothesis is based on the following observations from previous studies. First, olfactory nerve regeneration was delayed in apoE-gene knockout (KO) mice as compared to wild type (WT) mice. …

Fragmentation Effects On Fitness In Five Common Prairie Species, Lydia Miramontes Loyd

Masters Theses

Midwest tallgrass prairies have suffered extensive habitat loss and as a result persist as isolated and highly fragmented remnants. In such small, isolated remnants, genetic drift and inbreeding depression can lead to a loss of genetic variation over time. Reduced genetic variation is often associated with reduced levels of growth, survival, and reproduction as well as increased susceptibility to stress. This study examined fitness traits of five common prairie species to determine whether plants in small fragmented remnants had reduced fitness as compared to those in a larger remnant. Five prairie species were chosen for this study based on their …

Who's Afraid Of Polygamy? Exploring The Boundaries Of Family, Equality And Custom In South Africa, Penelope Andrews

Articles & Chapters

South Africa's post-apartheid constitution has been widely admired and constantly referenced by international scholars, and especially international human rights scholars, for its comprehensive embrace of gender equality. But the commitment to gender equality has been tested by other liberatory discourses, including African nationalism and cultural and religious autonomy. This Article examines the evolution of South African legislation and constitutional jurisprudence in the face of competing imperatives, for example, between equality, legal pluralism, customary law/religious law, and the recognition of polygamy. In particular, it focuses on the Recognition of Customary Marriages Act, a statute that purports to regulate customary marriages, including …

Spectrum 2009, Saint Mary's College Of California

The Undergraduate Spectrum

No abstract provided.