Digital Commons Network^™

The Interaction Between Kshv Rta And Cellular Rbp-Jκ And Their Subsequent Dna Binding Are Not Sufficient For Activation Of Rbp-Jκ, Anil Papugani, Tricia Coleman, Clinton J. Jones, Luwen Zhang

School of Veterinary and Biomedical Sciences: Faculty Publications

Kaposi’s sarcoma-associated herpesvirus (KSHV) replication and transcription activator (RTA) is necessary and sufficient for the switch from KSHV latency to lytic replication. RTA activates promoters by several mechanisms. RTA can bind to sequences in viral promoters and activate transcription. In addition, RTA interacts with the cellular recombination signal sequence-binding protein-J kappa (RBP- Jκ), a transcriptional repressor, converts the repressor into an activator and activates viral promoters via RBP- Jκ. Because RBP- Jκ is required for RTA to activate lytic replication, it is important to understand how RTA cooperates with RBP- Jκ protein to activate KSHV lytic replication program. Previously, we …

Premature Expression Of The Latency-Related Rna Encoded By Bovine Herpesvirus Type 1 Correlates With Higher Levels Of Beta Interferon Rna Expression In Productively Infected Cells, Sandra Perez, Florencia Meyer, Kazima Saira, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Bovine herpesvirus type 1 (BHV-1) is an important pathogen that can initiate bovine respiratory disease complex. Like other members of the subfamily Alphaherpesvirinae, BHV-1 establishes latency in sensory neurons. The latency-related (LR) gene expresses a family of alternatively spliced transcripts in infected sensory neurons that have the potential to encode several LR proteins. An LR mutant virus that contains three stop codons near the 5’ terminus of the first open reading frame in the LR gene does not express two LR proteins or reactivate from latency. In addition, the LR mutant virus induces higher levels of apoptosis in trigeminal …

A Methodology For Assessing Rural Livelihood Strategies In West/Central Africa: Lessons From The Field, Ruth Malleson, Stella Asaha, Terry Sunderland, Philip Burnham, Martins Egot, Kwaku Obeng-Okrah, Imabong Ukpe, Wendy Miles

Ecological and Environmental Anthropology (University of Georgia)

This paper critically evaluates and discusses some of the methodological practicalities of applying a combined participatory and small-scale survey approach to investigating rural livelihood strategies of people living in the humid forest zone of Southwest Cameroon, Southeast Nigeria and Southwest Ghana, with particular reference to assessing the economic importance of non-timber forest products. It describes the sampling methods used to select study zones, settlements and households as well as the participatory techniques and instruments used to differentiate households and gather information on rural incomes. Details of the successes and problems encountered during implementation are presented. The challenges faced by those …

Influence Of Fipronil Compounds And Rice-Cultivation Land-Use Intensity On Macroinvertebrate Communities In Streams Of Southwestern Louisiana, Usa, Scott V. Mize, Stephen D. Porter, Dennis K. Demcheck

United States Geological Survey: Staff Publications

Laboratory tests of fipronil and its degradation products have revealed acute lethal toxicity at very low concentrations (LC₅₀) of <0.5 μg/L to selected aquatic macroinvertebrates. In streams draining basins with intensive rice cultivation in southwestern Louisiana, USA, concentrations of fipronil compounds were an order of magnitude larger than the LC₅₀. The abundance (p=-0.64; p=0.015) and taxa richness (r²=0.515, p<0.005) of macroinvertebrate communities declined significantly with increases in concentrations of fipronil compounds and rice-cultivation land-use intensity. Macroinvertebrate community tolerance scores increased linearly (r²=0.442, p < 0.005) with increases in the percentage of rice cultivation in the basins, indicating increasingly degraded stream conditions. Similarly, macroinvertebrate community-tolerance scores increased rapidly as fipronil concentrations approached about 1 μg/L. Pesticide toxicity index determinations indicated that aquatic macroinvertebrates respond to a gradient of fipronil compounds in water although stream size and habitat cannot be ruled out as contributing influences.

Comparison Of Total Mercury And Methylmercury Cycling At Five Sites Using The Small Watershed Approach, James B. Shanley, M. Alisa Mast, Donald H. Campbell, George R. Aiken, David P. Krabbenhoft, Randall J. Hunt, John F. Walker, Paul F. Schuster, Ann Chalmers, Brent T. Aulenbach, Norman E. Peters, Mark Marvin-Dipasquale, David W. Clow, Martin M. Shafer

United States Geological Survey: Staff Publications

The small watershed approach is well-suited but underutilized in mercury research. We applied the small watershed approach to investigate total mercury (THg) and methylmercury (MeHg) dynamics in streamwater at the five diverse forested headwater catchments of the US Geological Survey Water, Energy, and Biogeochemical Budgets (WEBB) program. At all sites, baseflow THg was generally less than 1 ng L^-1 and MeHg was less than 0.2 ng L^-1. THg and MeHg concentrations increased with streamflow, so export was primarily episodic. At three sites, THg and MeHg concentration and export were dominated by the particulate fraction in association with …

Early Childhood Infection By Human Herpesvirus 8 In Zambia And The Role Of Human Immunodeficiency Virus Type 1 Coinfection In A Highly Endemic Area, Veenu Minhas, Kay L. Crabtree, Ann Chao, Tendai J. M'Sola, Chipepo Kankasa, Marc Bulterys, Charles D. Mitchell, Charles Wood

Nebraska Center for Virology: Faculty Publications

Kaposi’s sarcoma occurs at high incidence among Zambian adults and children, but there is a paucity of data on human herpesvirus 8 (HHV-8) incidence and routes of infection, especially in children. Between 1998 and 2004, the authors conducted a prospective study of viral transmission in a cohort of 684 children in Lusaka, Zambia, to estimate the annual incidence of HHV-8 from birth through 48 months of age. Maternal and pediatric human immunodeficiency virus type 1 (HIV-1) infection status was also determined. The results, based on 1,532 child-years of follow-up, showed that HHV-8 seroconversion occurs early in life. The incidence rate …

The Overmyer Mastodon (Mammut Americanum) From Fulton County, Indiana, Neal Woodman, Jon W. Branstrator

United States Geological Survey: Staff Publications

In June 1978 the partial skeleton of an American mastodon, Mammut americanum, was salvaged from a drainage ditch in Fulton County, north-central Indiana. The remains were recovered mostly from ca. 170–260 cm below the current land surface in marl overlain by peat and peaty marl. The stratigraphy of the site indicates that the remains were deposited in a small, open-water pond that subsequently filled. The skeleton, which is 41–48% complete, is that of a mature female, ca. 30–34 y old at death based on dental eruption and wear. Postcranial bone measurements indicate that this individual was relatively large for …

Effectiveness Of Lethal, Directed Wolf-Depredation Control In Minnesota, Elizabeth Harper, William J. Paul, L. David Mech, Sanford Weisberg

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Wolf (Canis lupus) depredations on livestock in Minnesota, USA, are an economic problem for many livestock producers, and depredating wolves are lethally controlled. We sought to determine the effectiveness of lethal control through the analysis of data from 923 government-verified wolf depredations from 1979 to 1998. We analyzed the data by 1) assessing the correlations between the number of wolves killed in response to depredations with number of depredations the following year at state and local levels, and 2) the time to the next depredation. No analysis indicated that trapping wolves substantially reduced the following year’s depredations at …

Ecosystem Services Derived From Wetland Conservation Practices In The United States Prairie Pothole Region With An Emphasis On The U.S. Department Of Agriculture Conservation Reserve And Wetlands Reserve Programs, Robert A. Gleason, Murray K. Laubhan, Brian A. Tangen, Kevin E. Kermes

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Contents:

Chapter A Background and Approach to Quantification of Ecosystem Services By Robert A. Gleason and Murray K. Laubhan

Chapter B Plant Community Quality and Richness By Murray K. Laubhan and Robert A. Gleason

Chapter C Carbon Sequestration By Robert A. Gleason, Brian A. Tangen, and Murray K. Laubhan

Chapter D Floodwater Storage By Robert A. Gleason and Brian A. Tangen

Chapter E Reduction of Sedimentation and Nutrient Loading By Brian A. Tangen and Robert A. Gleason

Chapter F Proposed Approach to Assess Potential Wildlife Habitat Suitability on Program Lands By Murray K. Laubhan, Kevin E. Kermes, and Robert A. …

Spatial Dynamics Of Overbank Sedimentation In Floodplain Systems, Aaron R. Pierce, Sammy L. King

United States Geological Survey: Staff Publications

Floodplains provide valuable social and ecological functions, and understanding the rates and patterns of overbank sedimentation is critical for river basin management and rehabilitation. Channelization of alluvial systems throughout the world has altered hydrological and sedimentation processes within floodplain ecosystems. In the loess belt region of the Lower Mississippi Alluvial Valley of the United States, channelization, the geology of the region, and past land-use practices have resulted in the formation of dozens of valley plugs in stream channels and the formation of shoals at the confluence of stream systems. Valley plugs completely block stream channels with sediment and debris and …

Linking Ecosystem Processes With Wetland Management Goals: Charting A Course For A Sustainable Future, Ned H. Euliss Jr., Loren M. Smith, Douglas A. Wilcox, Bryant A. Browne

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Wetland management in the United States has never been as challenging as in today’s highly modified landscape. Initially, wetland science and management emerged as professions in response to widespread conversion of wetlands to other uses and concerns over negative impacts on wildlife populations, especially migratory birds. Consequently, wetland management was focused on wildlife, and the initial management technique was simply to protect wetlands. However, extensive conversion of lands for agricultural and urban expansion over the past 60 years has modified ecosystem processes at the landscape scale sufficiently to compromise wetland management activities on adjacent lands dedicated to conservation. Moreover, society …

Integrated Modelling Frameworks For Environmental Assessment And Decision Support, A. E. Rizzoli, G. Leavesley, J. C. Ascough Ii, R. M. Argent, I. N. Athanasiadis, V. Brilhante, F. H. A. Claeys, O. David, M. Donatelli, P. Gijsbers, D. Havlik, A. Kassahun, P. Krause, N. W. T. Quinn, H. Scholten, R. S. Sojda, F. Villa

United States Geological Survey: Staff Publications

As argued in Chapter 1, modern management of environmental resources defines problems from a holistic and integrated perspective, thereby imposing strong requirements on Environmental Decision Support Systems (EDSSs) and Integrated Assessment Tools (IATs). These systems and tools tend to be increasingly complex in terms of software architecture and computational power in order to cope with the type of problems they must solve. For instance, the discipline of Integrated Assessment (IA) needs tools that arc able to span a wide range of disciplines, from socio-economics to ecology to hydrology. Such tools must support a wide range of methodologies and techniques like …

Time Perspectivism, Temporal Dynamics, And Battlefield Archaeology: A Case Study From The Santiago Campaign Of 1898, William E. Altizer

Nebraska Anthropologist

Given the unique ability of archaeology to illuminate temporal processes, archaeologists have begun employing a number of theoretical models to understand the nature of these processes, and the ways in which the modern archaeological landscape retains their physical traces. Battlefields, as discrete temporal events taking place in physical settings with their own history, offer an intriguing avenue to explore archaeological temporality. This paper reviews the ways in which archaeologists have employed the Annales approach and time perspectivism, and considers a case study in battlefield archaeology - the 1898 battlefield of EI Caney, Cuba.

The "How" Of The Three Sisters: The Origins Of Agriculture In Mesoamerica And The Human Niche, Amanda J. Landon

Nebraska Anthropologist

The origins of agriculture· in Mesoamerica have long interested archaeologists and antiquarians alike. The approaches used to understand the origins of the three sisters, maize, beans and squash, have changed over time as our understanding of the ecological context and ethnographic influences have changed. In this paper, I examine the history of the study of the origins of agriculture and assess the current evolutionary and ecological approaches to the topic. In Mesoamerica, the three sisters and humans shared a co evolutionary relationship in which humans invited the plants into the human niche and the plants thrived. Over time, the plants …

Physicians And Execution: Highlights From A Discussion Of Lethal Injection, Deborah W. Denno

Faculty Scholarship

No abstract provided.

The Empirics Of Prison Growth: A Critical Review And Path Forward, John F. Pfaff

Faculty Scholarship

A growing empirical literature has sought to explain the forces behind the significant expansion of the U.S. prison population over the past thirty years. Unfortunately, the studies to date have suffered from important methodological, conceptual, and definitional problems that have significantly curtailed their ability to identify causal effects. In this Article, I examine several of the central limitations and discuss remedies. I start by reviewing the theories that investigators have sought to test. I then discuss the studies' empirical defects, such as failing to account for endogenous relationships, overlooking the risk of model dependency, ignoring complex dynamic relationships, using variables …

Metals Fate And Transport Modelling In Streams And Watersheds: State Of The Science And Usepa Workshop Review, B. S. Caruso, T. J. Cox, R. L. Runkel, M. L. Velleux, K. E. Bencala, D. K. Nordstrom, P. Y. Julien, B. A. Butler, Charles N. Alpers, A. Marion, K. S. Smith

United States Geological Survey: Staff Publications

Metals pollution in surface waters from point and non-point sources (NPS) is a widespread problem in the United States and worldwide (Lofts et al., 2007; USEPA, 2007). In the western United States, metals associated with acid mine drainage (AMD) from hardrock mines in mountainous areas impact aquatic ecosystems and human health (USEPA, 1997a; Caruso and Ward, 1998; Church et al., 2007). Metals fate and transport modelling in streams and watersheds is sometimes needed for assessment and restoration of surface waters, including mining-impacted streams (Runkel and Kimball, 2002; Caruso, 2003; Velleux et al., 2006). The Water Quality Analysis …

Comparison Of The Usgs 2001 Nlcd To The 2002 Usda Census Of Agriculture For The Upper Midwest United States, S.K. Maxwell, E.C. Wood, A. Janus

United States Geological Survey: Staff Publications

The U.S. Geological Survey (USGS) 2001 National Land Cover Database (NLCD) was compared to the U.S. Department of Agriculture (USDA) 2002 Census of Agriculture. We compared areal estimates for cropland at the state and county level for 14 States in the Upper Midwest region of the United States. Absolute differences between the NLCD and Census cropland areal estimates at the state level ranged from 1.3% (Minnesota) to 37.0% (Wisconsin). The majority of counties (74.5%) had differences of less than 100 km². 7.2% of the counties had differences of more than 200 km². Regions where the largest …

Foot-And-Mouth Disease In North American Bison (Bison Bison) And Elk (Cervus Elaphus Nelsoni): Susceptibility, Intra- And Interspecies Transmission, Clinical Signs, And Lesions, Jack Rhyan, Ming Deng, He Wang, Gordon Ward, Thomas Gidlewski, Matthew Mccollum, Samia Metwally, Thomas Mckenna, Sherrilyn Wainwright, Antonio Ramirez, Charles Mebus, Mo Salman

United States Department of Agriculture Wildlife Services: Staff Publications

There is limited information about the pathogenesis and epidemiology of foot-andmouth disease (FMD) in North American bison (Bison bison) or elk (Cervus elaphus nelsoni). In these two experimental infection studies, we compared the susceptibilities of bison and elk to FMD virus (FMDV), respectively, with that of cattle; determined whether intra- and interspecies transmission could occur in bison and cattle, and elk and cattle; determined suitability of conventional available laboratory tests to detect FMDV infection in bison and elk; and investigated whether bison or elk are efficient long-term carriers of FMDV. In both studies, after a period …

Are There Π* Shape Resonances In Electron Scattering From Phosphate Groups?, Paul Burrow, Gordon A. Gallup, Alberto Modelli

Paul Burrow Publications

The temporary anion states of trimethyl phosphate and several compounds bearing the P=O group were explored using electron transmission spectroscopy and ab initio calculations to determine if these states have the characteristics of the π* resonances usually associated with multiple bonds. No evidence was found for this in (CH₃O)₃PO and, by extension, we do not expect them to appear in the phosphate group of DNA. Cl₃PO, however, does display such characteristics to some extent, and we show that they arise from the spatial properties of the π* (P-Cl) orbitals rather than from multiple PO …

Oligo(Vinylidene Fluoride) Langmuir-Blodgett Films Studied By Spectroscopic, Rafal Korlacki, J. Travis Johnston, Jihee Kim, Stephen Ducharme, Daniel W. Thompson, Vladimir M. Fridkin, Zhongxin Ge, James M. Takacs

Stephen Ducharme Publications

Thin films of amphiphilic vinylidene fluoride oligomers prepared by Langmuir–Blodgett deposition on silicone substrates were investigated by comparing experimental and theoretical mid-infrared (IR) spectra. The experimental spectra were obtained using infrared spectroscopic ellipsometry. Theoretical spectra were calculated using density functional theory. Excellent correspondence of major IR bands in both data sets shows that the molecular backbone is oriented with the long axis normal to the substrate plane. This is in contrast to poly vinylidene fluoride[1] LB films, in which the polymer chains are parallel to the substrate.

Hamulonema Gen. Nov. For Teladorsagia Hamata And Ostertagia Kenyensis In The Ostertagiinae Fauna (Nematoda: Trichostrongyloidea) From African Ungulates, Eric P. Hoberg, Arthur Abrams

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Hamulonema gen. nov. is proposed for Teladorsagia hamata and Ostertagia kenyensis in the ostertagiine nematode fauna found in artiodactyl hosts from Africa. Monomorphic species representing this genus are characterized by a bilaterally symmetrical and parallel synlophe in males and females, a 2-2-1 bursal formula, an accessory bursal membrane that is strongly cuticularized and reduced, a strongly reduced dorsal lobe and ray, and robust spicules with a simple, weakly pointed, ventral process, and curved, hooklike dorsal process. Species referred to Hamulonema nov. gen. are immediately distinguished from those of Camelostrongylus, Longistrongylus, Marshallagia, Orloffia, Ostertagia, and Pseudomarshallagia …

Casnr Salute To Graduates, John Owens

John Owens: Speeches and Appearances

We appreciate your being with us this afternoon as we celebrate the accomplishments of tomorrow's graduates from the University of Nebraska-Lincoln. Thank you for attending and, congratulations to the class of 2008! This ceremony has become both a fixture and a tradition in the College of Agricultural Sciences and Natural Resources. As a matter of fact, it took the national "First Place Award" in a 2006 competition that was open to all universities in the United States.

Minimal Nutrition Intervention With High-Protein/Low-Carbohydrate And Low-Fat, Nutrient-Dense Food Supplement Improves Body Composition And Exercise Benefits In Overweight Adults: A Randomized Controlled Trial, Christopher M. Lockwood, Jordan R. Moon, Sarah E. Tobkin, Ashley A. Walter, Abbie E. Smith, Vincent J. Dalbo, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Exercise and high-protein/reduced-carbohydrate and -fat diets have each been shown separately, or in combination with an energy-restricted diet to improve body composition and health in sedentary, overweight (BMI > 25) adults. The current study, instead, examined the physiological response to 10 weeks of combined aerobic and resistance exercise (EX) versus exercise + minimal nutrition intervention designed to alter the macronutrient profile, in the absence of energy restriction, using a commercially available high-protein/low-carbohydrate and low-fat, nutrient-dense food supplement (EXFS); versus control (CON).

Methods: Thirty-eight previously sedentary, overweight subjects (female = 19; male = 19) were randomly assigned to either CON (n …

The Effect Of Beta-Alanine Supplementation On Neuromuscular Fatigue In Elderly (55–92 Years): A Double-Blind Randomized Study, Jeffrey R. Stout, B. Sue Graves, Abbie E. Smith, Michael J. Hartman, Joel T. Cramer, Travis W. Beck, Roger C. Harris

Department of Nutrition and Health Sciences: Faculty Publications

Background: Ageing is associated with a significant reduction in skeletal muscle carnosine which has been linked with a reduction in the buffering capacity of muscle and in theory, may increase the rate of fatigue during exercise. Supplementing beta-alanine has been shown to significantly increase skeletal muscle carnosine. The purpose of this study, therefore, was to examine the effects of ninety days of beta-alanine supplementation on the physical working capacity at the fatigue threshold (PWC_FT) in elderly men and women.

Methods: Using a double-blind placebo controlled design, twenty-six men (n = 9) and women (n = 17) (age ± …

Presidential Address: Nothing Succeeds Like Excess, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

To the members of the American Society of Parasitologists, I offer my appreciation for the opportunity to serve as your president. When I was notified of my successful election as an officer, it brought to mind that famous remark by Adlai Stevenson who said, ‘‘In America, anybody can be president. That’s one of the risks you take.’’ So, what I initially perceived as somewhat of a risky venture, proved to be a more valuable and interesting experience than I anticipated. Let’s hope that my presidential address has a similar outcome.

The Kinetic Energy Dependent Effective Debye Temperature For Cos2 (100), Ning Wu, David Wisbey, Takashi Komesu, Z. X. Yu, M. Manno, L. Wang, C. Leighton, Peter A. Dowben

Peter Dowben Publications

The effective Debye temperatures of the highly spin-polarized material CoS₂ were measured using temperature dependent low energy electron diffraction and shown to be dependent upon electron kinetic energy. The normal dynamic motion of the (100) surface results in the effective surface Debye temperature of 326 ± 9 K compared to a bulk Debye temperature of 612 ± 24 K. Similar values for the bulk Debye temperature have been obtained through LEED I(V) analysis, core level photoemission with a lower value for the bulk Debye temperature found from heat capacity measurements.

The Surface Stability Of Cos2(100), Ning Wu, Renat F. Sabirianov, Chun-Gang Duan, Wai-Ning Mei, David Wisbey, Yaroslav B. Losovyj, M. Manno, C. Leighton, En Cai, Jiandi Zhang, Peter A. Dowben

Peter Dowben Publications

The stability of various possible terminations of the CoS₂ (1 × 1) surface have been explored and theoretical expectations are found to agree with experiment. With extensive annealing, there is a phase separation at the (100) surface of CoS₂. Sulfur segregation to the surface leads to a significant change in the largely sulfur bands due to changes in the hybridized bands, with cobalt. Resonant photoemission spectra indicate clearly that the hybridized cobalt and sulfur bands, characteristic of the CoS₂ bulk, lie at higher binding energies than those of segregated sulfur layers. This is discussed in terms …

The Cms Experiment At The Cern Lhc, S. Chatrchyan, Kenneth A. Bloom, Brian Bockelman, Daniel R. Claes, Aaron Dominguez, Michael Eads, Makoto Furukawa, J. Keller, T. Kelly, Carl Lundstedt, Sudhir Malik, Gregory Snow, David R. Swanson, Cms Collaboration

Kenneth Bloom Publications

The Compact Muon Solenoid (CMS) detector is described. The detector operates at the Large Hadron Collider (LHC) at CERN. It was conceived to study proton-proton (and leadlead) collisions at a centre-of-mass energy of 14 TeV (5.5 TeV nucleon-nucleon) and at luminosities up to 10³⁴ cm^-2s^-1 (10²⁷ cm^-2s^-1). At the core of the CMS detector sits a high-magnetic field and large-bore superconducting solenoid surrounding an all-silicon pixel and strip tracker, a lead-tungstate scintillating-crystals electromagnetic calorimeter, and a brass-scintillator sampling hadron calorimeter. The iron yoke of the flux-return is instrumented with four stations …

Economic Analysis Of Labor And Employment Law In The New Economy, Kenneth G. Dau-Schmidt, Alan Hyde, Michael Risch, Jagdeep Bhandari, Richard Block

Articles by Maurer Faculty

No abstract provided.