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Bio 1004g : Practical Microbiology, Biological Sciences

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Habitat Condition And Associated Macrofauna Reflect Differences Between Protected And Exposed Seagrass Landscapes, Chet F. Rakocinski, Cynthia A. Moncreiff, Mark S. Peterson, Katherine E. Vanderkooy, Todd A. Randall

Gulf and Caribbean Research

Seagrass landscape configurations associated with different physical settings can affect habitat-structure and plant-animal relationships. We compared shoal grass (Halodule wrightii) habitat and macrofaunal variables between two fragmented seagrass landscapes at barrier-island locations subject to different disturbance regimes. Five seagrass habitat variables including above ground biomass (AGB), shoot number, per shoot biomass, epiphyte biomass and below ground biomass (BGB), differed significantly between the island landscapes. Per shoot biomass and epiphyte biomass also varied significantly over the seagrass growing season; and epiphyte biomass showed a strong landscape-time interaction. Abundances of microgastropods normalized to AGB differed significantly between landscapes. An inverse …

Seasonal Patterns Of Juvenile Fish Abundance In Seagrass Meadows In Teague Bay Bank Barrier Reef Lagoon, St. Croix, U.S. Virgin Islands, Ivan Mateo, William J. Tobias

Gulf and Caribbean Research

Considerable knowledge has been gained regarding fish use of nearshore habitats such as seagrass meadows or mangrove lagoons in the Caribbean (e.g., evaluation of nursery value, trophic linkages). However, few studies have been conducted on fish recruitment to seagrass habitat around the Caribbean. Juvenile reef fish in seagrass meadows at Teague Bay, St. Croix, U.S. Virgin Islands were surveyed from October 1998 through September 1999 using a visual census technique. Grunts (Haemulidae) were the most abundant juveniles observed (60% of all fish), followed by wrasses (Labridae, 20%) and parrotfishes (Scaridae, 13%). French grunt, Haemulon flavolineatum, were the most numerous …

An Unusual Reaction And Other Observations Of Sperm Whales Near Fixed-Wing Aircraft, Mari A. Smultea, Joseph R. Mobley Jr., Dagmar Fertl, Gregory L. Fulling

Gulf and Caribbean Research

Reported behavioral reactions by sperm whales to aircraft are sparse, highly variable, and largely anecdotal as summarized below. Observers since the whaling era began have noted that sperm whales tend to be skittish (Whitehead 2003). When documented, sperm whale reactions to both planes and helicopters range from no reaction (Clarke 1956, Gambell 1968, Green et al. 1992) to reactions such as increased surface intervals and dramatic behavioral changes (Clarke 1956, Fritts et al. 1983, Mullin et al. 1991, Würsig et al. 1998, Richter et al. 2003, 2006). Given the lack of supporting data for either case, it is important that …

Documentation Of A Gulf Sturgeon Spawning Site On The Yellow River, Alabama, Usa, Brian R. Kreiser, J. Berg, M. Randall, F. Parauka, S. Floyd, B. Young, K. Sulak

Gulf and Caribbean Research

The Gulf Sturgeon Recovery Plan (USFWS, GSMFC and NMFS 1995) stressed the need to provide maximum protection to Gulf sturgeon spawning habitat. The approach employed by various Gulf sturgeon researchers, including ourselves, to document spawning has been to identify potential spawning habitat on the basis of physical characteristics and/or tracking data, collect eggs, and then raise the eggs in the laboratory until the point where the larval fish can be identified (e.g., Marchant and Shutters 1996, Sulak and Clugston 1998, 1999). However, collecting eggs in any appreciable number is usually difficult, and these eggs may not always be viable upon …

Absorption Spectral Slopes And Slope Ratios As Indicators Of Molecular Weight, Source, And Photobleaching Of Chromophoric Dissolved Organic Matter, John R. Helms, Aron Stubbins, Jason D. Ritchie, Elizabeth C. Minor, David J. Kieber, Kenneth Mopper

Chemistry & Biochemistry Faculty Publications

A new approach for parameterizing dissolved organic matter ( DOM) ultraviolet-visible absorption spectra is presented. Two distinct spectral slope regions ( 275-295 nm and 350-400 nm) within log-transformed absorption spectra were used to compare DOM from contrasting water types, ranging from wetlands (Great Dismal Swamp and Suwannee River) to photobleached oceanic water ( Atlantic Ocean). On the basis of DOM size-fractionation studies ( ultrafiltration and gel filtration chromatography), the slope of the 275-295- nm region and the ratio of these slopes (S_R; 275-295- nm slope : 350-400- nm slope) were related to DOM molecular weight ( MW) and …

Comparison Of Silica Polyamine Composites And Crosslinked Polystyrene Resins, Jessica Lee Wood

Graduate Student Theses, Dissertations, & Professional Papers

The development of silica polyamine composites (SPC) was inspired by the need for more environmentally benign ways of extracting and recovering technologically important and environmentally dangerous metals from waste streams. These solid phase adsorbents are designed to endure the extreme conditions associated with environmental remediation and the metal processing industry. In order for the silica polyamine composites to be viable in industry, their performance must meet or exceed the current methods of metal extraction from aqueous media. A study was undertaken to compare three silica polyamine composites: WP-2, BPAP, and CuWRAM, with their closest crosslinked polystyrene resin analogs. The studies …

Hierarchical Summer Habitat Selection By The North American Porcupine In Western Montana, Katie Ann Mally

Graduate Student Theses, Dissertations, & Professional Papers

The North American porcupine (Erethizon dorsatum) has continually been a species of low priority for research and conservation efforts in the western United States. While many porcupine populations have been studied in North America, little to no research has been done on porcupines in western Montana or habitats of similar composition. More recently it has been noted that porcupine sightings have become rare in western Montana is areas where sightings were once common. Due to the overall lack of information about western porcupine populations it is imperative to gather basic natural history information in order to make informed management decisions. …

Studies Of The Dimerization And Packaging Signals In Hiv-2 Rna, Tayyba Tabassum Baig

Graduate Student Theses, Dissertations, & Professional Papers

The investigation of sequences and structures in the 5' untranslated leader region (5'UTR) of HIV genomic RNA is essential for understanding viral replication because the 5'UTR regulates several essential functions by the alternate presentation and sequestration of signals through conformational changes. Our main focus in this study was to understand those sequences and structures that are involved in dimerization and packaging of HIV-2 RNA. Progressing from previous findings, we studied in detail a 10-nucleotide palindrome sequence (pal; 5'-GGAGUGCUCC-3') of the 5'UTR, located within the major packaging signal, upstream of the dimerization signal (SL1: stem loop-1). Pal has been shown to …

"Words Are No Good": The Curse Of Signification And The Curse Of Faulkner's South, Michael Joseph St. Thomas

Graduate Student Theses, Dissertations, & Professional Papers

In this study I examine three of Faulkner’s novels that concern his fictional Yoknapatawpha County: As I Lay Dying (1930), Absalom, Absalom! (1936), and Go Down, Moses (1942). These novels, I argue, indicate a development in Faulkner’s relationship to the formalist hierarchy of art over real life. To show this development I will investigate the topic of language as an inadequate medium in characters’ relationships to nature and the past. In As I Lay Dying Faulkner presents words as something unable to achieve the transcendence his characters desire. In Absalom, Absalom! and Go Down, Moses the author extends this suspicion …

Kinetics And Thermodynamics Of Salt-Dependent T7 Gene 2.5 Protein Binding To Single- And Double-Stranded Dna., Leila Shokri, Boriana Marintcheva, Mootaz Eldib, Andreas Hanke, Ioulia Rouzina, Mark C. Williams

Physics & Astronomy Faculty Publications

Bacteriophage T7 gene 2.5 protein (gp2.5) is a single-stranded DNA (ssDNA)-binding protein that has essential roles in DNA replication, recombination and repair. However, it differs from other ssDNA-binding proteins by its weaker binding to ssDNA and lack of cooperative ssDNA binding. By studying the rate-dependent DNA melting force in the presence of gp2.5 and its deletion mutant lacking 26 C-terminal residues, we probe the kinetics and thermodynamics of gp2.5 binding to ssDNA and double-stranded DNA (dsDNA). These force measurements allow us to determine the binding rate of both proteins to ssDNA, as well as their equilibrium association constants to dsDNA. …

Ralph L. Daniels V. Gamma West Brachytherapy Llc And John K. Hayes, M.D. : Brief Of Appellant, Utah Supreme Court

Utah Supreme Court Briefs (2000–2020)

APPEAL FROM THE THIRD DISTRICT COURT, SALT LAKE COUNTY, STATE OF UTAH, THE HONORABLE ROBIN W. REESE PRESIDING

Microassembly Technology For Modular, Polymer Microfluidic Devices, Byoung Hee You

LSU Doctoral Dissertations

Assembly of modular, polymer microfluidic devices with different functions to obtain more capable instruments may significantly expand the options available for detection and diagnosis of disease through DNA analysis and proteomics. For connecting modular devices, precise, passive alignment structures can be used to prevent infinitesimal motions between the devices and minimize misalignment. The motion and constraint of passive alignment structures were analyzed using screw theory. A combination of three v-groove and hemisphere-tipped post joints constrained all degrees of freedom of the two mating modules without overconstraint. Simulations and experiments were performed to assess the predictability of dimensional and location variations …

Designing Surface Chemistries For In Situ Afm Investigations Of Biomolecular Reactions With Proteins At The Nanoscale, Johnpeter Ndiangui Ngunjiri

LSU Doctoral Dissertations

In situ atomic force microscopy (AFM) characterizations and lithography can be applied to investigate the orientation, reactivity and stability of protein molecules adsorbed on nanostructures of self-assembled monolayers at near-physiological conditions. Automated nanografting was used to fabricate regular arrays of nanopatterns of ù-functionalized n-alkanethiols with designated terminal chemistries. After writing nanopatterns, protein binding occurs selectively on carboxylate-terminated nanopatterns via covalent bonds that are formed using N-ethyl-N'(dimethylaminoporpyl)-carbodiimide and N-hydroxysuccinimide activation. The amine groups of lysine residues of proteins bond covalently to nanopatterns of carboxylate-terminated alkanethiol self-assembled monolayers, to form a robust surface attachment for sustained contact-mode AFM imaging during biochemical reactions. …

Evolutionary History And Hybridization In Passerina Buntings, Matthew D. Carling

LSU Doctoral Dissertations

Understanding the genetic basis of speciation is of fundamental importance to evolutionary biology and hybrid zones offer unique natural laboratories in which to investigate the ecological and evolutionary processes important in creating and maintaining biological diversity. By comparing introgression patterns of different loci, researchers can begin to identify genetic regions that contribute to reproductive isolation between hybridizing taxa. In taxa, like birds, with heterogametic females, Haldane’s rule predicts that mtDNA and z-linked loci will introgress less than autosomal loci. I tested this prediction using the hybrid zone between Passerina cyanea (Indigo Bunting) and Passerina amoena (Lazuli Bunting), two species that …

Assessment Of Oxygen Sources And Sinks In The Northern Gulf Of Mexico Using Stable Oxygen Isotopes, Zoraida Jazmin Quinones-Rivera

LSU Doctoral Dissertations

Coastal hypoxia (< 2 mg O2L-1) represents a global problem that continues to worsen as nutrient fluxes to these areas increase. The second largest zone of human-induced hypoxia is located on the Louisiana continental shelf where hypoxic bottom waters commonly occur during summertime. This region is strongly impacted by the large flux of freshwater and nutrients from the Mississippi River, which influences both biological and physical processes that control oxygen dynamics. Yet, based on oxygen concentration measurements alone, it is difficult to separate the effects of biological factors from physical factors. To address this problem, I used a dual budget approach to assess the importance of oxygen sources and sinks on the Louisiana continental shelf. The dual budget was based on using stable oxygen isotopes (ä18O) in combination with conventional oxygen concentration measurements. To analyze temporal trends, surface and bottom water samples were collected monthly between July 2001 and July 2003 along an onshore-offshore transect. For better spatial resolution, shelfwide sampling was conducted extending from the Mississippi River Delta to the Louisiana-Texas border in the month of July of 2001, 2002, and 2003. Oxygen saturations values ranged between 180% at the surface and almost 0% close to the bottom with a corresponding range of ä18O values from 15‰ to 50‰. Biological parameters were important during all seasons for surface oxygen dynamics. The effects of physical factors were less apparent, except during severe physical disturbances. Bottom water oxygen dynamics showed clear seasonal signals of high oxygen depletion and larger contributions of benthic respiration during the summer, which corresponded to the strong stratification of the water column. In bottom waters, summer oxygen depletion was predominantly due to benthic respiration, accounting for about 73%, 80% and 60% of the total oxygen loss for 2001, 2002 and 2003 respectively. Model estimates of production/respiration (P/R) ratio during the July shelfwide cruises indicated that surface waters were productive with an average calculated P/R above 1. Depth stratified sampling (5 m intervals), which started in July 2002, showed that productivity in the mixed layer (5 to 10 m) was not homogeneous. Calculated P/R exceeded 1 only in the surface layer, while at 5 m P/R was approximately 1 and at a depth of 10 m, P/R was less than 1. Additionally, hypoxic conditions were only detected within 5 m of the bottom sediments. The dual budget approach yielded new estimates of productivity dynamics in surface waters and of sediment oxygen demand in bottom waters. For the first time, this study provided routine insight into productivity and respiration dynamics over large temporal and spatial scales. This could not have been accomplished using traditional methods because they commonly rely on time-consuming incubations. The study has shown that respiration dynamics in bottom waters vary seasonally with higher contribution of benthic respiration during stratified summer conditions and prevalent water column respiration during fall and winter. In contrast, seasonality in surface waters was less pronounced as productivity was more dependent on (salinity-inferred) nutrient supply than climatic forcing.

Bioanalytical Methods For Studies Of Homocysteine And Novel Cardiovascular Disease Indicators, Arther T. Gates

LSU Doctoral Dissertations

This dissertation explores the development of analytical methods for studies of CVD biomarkers and related biomolecular indicators. Initially, spectroscopic studies were conducted to investigate the chemical reactivity of homocysteine (Hcy), an independent CVD risk factor and serological biomarker. Consequently, we proposed an alternate theory for in vivo Hcy clearance based on spontaneous pyridoxal tetrahydrothiazine (PT) formation from Hcy and pyridoxal. The validity of PT-assisted Hcy clearance was further evaluated by use of capillary electrophoretic methods, which allowed rapid monitoring of protein oligomerization in PT-protein reaction mixtures. The results of these studies suggest that PT formation is a plausible mechanism for …

Wax Extraction And Characterization From Full-Fat And Defatted Rice Bran, Junghong Kim

LSU Doctoral Dissertations

Rice bran oil (RBO) contains 3-4% waxes (rice bran wax, RBW), which are composed of wax esters (WE), hydrocarbons, and other minor constituents. Saponified rice bran wax esters (RBWE) generate fatty acids, long chain alcohols, and phytosterols. Phytosterols and long chain fatty alcohols (policosanol) are known to reduce serum cholesterol and inhibit hepatic cholesterol synthesis. The yields and RBWE contents of RBO and RBW extracted from full-fat RB (FFRB) and defatted RB (DFRB) were determined using 6 different conditions with Soxhlet and Microwave-assisted extraction (MAE). The compositions of WEs from RBO and RBW were also compared. RBW was obtained from …

Solution Conformations And Structural Thermodynamics Of Type I Dna Polymerases From Escherichia Coli And Thermus Aquaticus, Allison Joubert Richard

LSU Doctoral Dissertations

The global conformations of DNA polymerase I from Thermus aquaticus (Taq/Klentaq) and E. coli (Pol I/Klenow) both in isolation and in complex with DNA have been examined in solution using hydrodynamic (analytical ultracentrifugation) and small/wide angle X-ray scattering techniques and then compared to their known crystal structures to assess the similarities and differences in the overall structure of these enzymes and enzyme complexes within a solution environment. These studies address the orientation of the 5’ nuclease domain with respect the polymerase domain (elongated or compact) for the full-length polymerases, and the binding mode (“polymerization” versus “editing”) of the DNA substrate …

Multimedia Mathematics Intervention For Math-Delayed Middle School Students, Lisa L. Stokes

LSU Doctoral Dissertations

The purpose of this study is to determine if the Sharpening Math Skills Lab technology-mediated mathematics instructional practices for math-delayed middle school students have positive effects on their mathematics achievement and spatial visualization ability and to gauge student engagement in learning, implementation of the principles of instructional design, and attitudes toward mathematics instruction. The results of a recent meta-analysis report a range of significantly positive to significantly negative effect sizes which establish a need for further evaluation of academic achievement utilizing technology-mediated mathematics programs at the middle school level (Slavin, Lake, & Groff, 2007). The literature (Moreno & Mayer, 2000) …

Cluster Kinetics Modeling Of Glassforming Materials, Lisa Ann Brenskelle

LSU Doctoral Dissertations

In this work, a new model relating temperature and pressure to dielectric relaxation or viscosity of a glassformer is developed. The model is based upon cluster kinetics, reaction-like mechanisms describing interactions between glassformer monomers and their clusters. Mathematical solutions of population balance equations for monomer and cluster lead to molar concentrations in terms of rate coefficients of the reaction-like mechanisms. These are then related to viscosity or dielectric relaxation time through free volume theory. The resulting equations are tested against data for a variety of pure glassformers, fragile, non-fragile, large, and small molecules over a wide range of temperatures, pressures, …

Synthesis And Surface Characterization Of Silica-Polypeptide Composite Particles, Erick Isael Soto-Cantu

LSU Doctoral Dissertations

The synthesis and sequential surface characterization of silica-polypeptide composite particles is described. Nearly monodisperse colloidal silica cores were obtained by the alkaline hydrolysis of tetraethyl orthosilicate (TEOS). The hydrodynamic radius can be easily controlled in the range of ƒî 20-140 nm by the water/TEOS ratio in the starting reaction mixture. The surface of the synthesized silica cores was further passivated/functionalized (P/F) by a mixture of (3-aminopropyl) trimethoxysilane (APS) and methyl-trimethoxysilane (MTMS). The amino groups were quantified using UV-Vis spectrometry after reaction with ninhydrin. The use of zeta potential measurements of the P/F silica particles at low pH is presented as …

Genetic And Mechanistic Analysis Of Heterochromatin Spreading In The Yeast S.Cerevisiae, Nithya Jambunathan

LSU Doctoral Dissertations

RNA Polymerase III transcribed tRNA genes are implicated in a wide variety of chromosome organizational functions that includes the ability to act as a boundary to heterochromatic silencing. A tRNA gene has been shown to be a major component of the barrier that prevents spreading of silencing from the HMR locus to the downstream GIT1 gene on chromosome III in S.cerevisiae. Our results suggest that additional proteins are involved in maintaining the boundary function of this tRNA gene. Mutations or deletions of the genes coding for these additional proteins have been shown to either weaken the boundary or enhance silencing. …

A Novel Automated Approach Of Multi-Modality Retinal Image Registration And Fusion, Hua Cao

LSU Doctoral Dissertations

Biomedical image registration and fusion are usually scene dependent, and require intensive computational effort. A novel automated approach of feature-based control point detection and area-based registration and fusion of retinal images has been successfully designed and developed. The new algorithm, which is reliable and time-efficient, has an automatic adaptation from frame to frame with few tunable threshold parameters. The reference and the to-be-registered images are from two different modalities, i.e. angiogram grayscale images and fundus color images. The relative study of retinal images enhances the information on the fundus image by superimposing information contained in the angiogram image. Through the …

Replication Of Viruses Responsible For Sweet Potato Virus Disease In Resistant And Susceptible Sweepotato Genotypes And Identification Of Molecular Markers Linked To Resistance, Douglas Watuku Miano

LSU Doctoral Dissertations

Virus diseases are a major constraint to sweetpotato production in East Africa. The most important is the Sweet potato virus disease (SPVD), a result of co-infection of Sweet potato chlorotic stunt virus (SPCSV) and Sweet potato feathery mottle virus (SPFMV). Studies were done on different aspects of resistance to SPVD, and to determine the presence of Sweet potato virus G (SPVG), Sweet potato virus 2 (SPV2), and Sweet potato leaf curl virus (SPLCV), viruses that have not been reported infecting sweetpotato in Kenya. None of the samples reacted to antisera for either SPVG or SPV-2. SPLCV was detected infecting sweetpotato …

Enzymatic Production And Biological Activities Of Cellobio-Oligosaccharides From Lignocellulose, Misook Kim

LSU Doctoral Dissertations

Cellulose is the most abundant biopolymer in nature and is the major component of lignocellulosic biomass. It has potential to produce not only glucose but value¨Cadded products such as cellobiose and oligosaccharides. Typically, enzymatic hydrolysis of cellulose produces glucose and a small amount of cellobiose. Modification of cellulase solutions altered ratio of glucose to cellobiose produced. The addition of glucose oxidase, gluconolactone, or gluconic acid significantly increased the amount of cellobiose remaining at the end of cellulose hydrolysis. Addition of glucose oxidase resulted in cellobiose being 23.7% of the product sugars from pure cellulose and 14.1% from ligonocellulose (sugarcane bagasse). …

The Influence Of Timed Reading Practices On Reading Gains And Comprehension Levels Of Third Grade Readers, Kristi D. Stuck

Theses and Dissertations

Since the signing of the No Child Left Behind (NCLB) Act, the Dynamic Indicators of Basic Early Literacy Skills (DIBELS) has been used by many schools to document students' reading gains. The purpose of the study was to investigate the influence of timed reading practices on the reading levels and comprehension scores of third grade students as they participated in literature discussion groups. Data collection included: reading levels from Developmental Reading Assessment, students' reading reflection logs, and transcriptions of language patterns during literature discussion groups. The findings revealed no statistical significance for students who participated in timed reading drills in …

Msp Possible Strands Framework, Maya Isabel Lazzaro, Mathematics And Science Partnerships

Long Term Ecological Research Network

Document of "Possible Strands Framework" 2008

Includes Essential Tasks for Year 1 and Suggested Activities at PI Meeting.

Differences In Methamphetamine Users And Crack Cocaine Users, Kilby Erwin Raptopoulos

Theses and Dissertations

This research addresses basic differences between crack cocaine users and methamphetamine users. The sample consisted of stimulant users who tested positive for either crack cocaine and/or methamphetamine from three rural counties in eastern Arkansas. This study seeks to further existing knowledge concerning drug use by examining demographics, drug use patterns, criminal histories and parental substance abuse. The study used Respondent-Driven Sampling to accumulate a sample size of 211 participants. Data was obtained through self-reported demographic information, drug use, criminal histories, and parental substance abuse problems during individual interviews, which were also matched with the results from a urinary analysis to …

Methods In Industrial Biotechnology For Chemical Engineers, Florentin Smarandache, W.B. Vasantha Kandasamy

Branch Mathematics and Statistics Faculty and Staff Publications

Industrial Biotechnology is an interdisciplinary topic to which tools of modern biotechnology are applied for finding proper proportion of raw mix of chemicals, determination of set points, finding the flow rates etc., This study is significant as it results in better economy, quality product and control of pollution. The authors in this book have given only methods of industrial biotechnology mainly to help researchers, students and chemical engineers. Since biotechnology concerns practical and diverse applications including production of new drugs, clearing up pollution etc. we have in this book given methods to control pollution in chemical industries as it has …