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A Kinetic Model For Growth And Biosynthesis Of Medium-Chain-Length Poly-(3-Hydroxyalkanoates) In Pseudomonas Putida, Mohamad Suffian Mohamad Annuar, Irene Kit Ping Tan, Shaliza Ibrahim, K.B. Ramachandran

Research Publications (2006 to 2010)

A kinetic model is presented giving a mathematical description of batch culture of Pseudomonas putida PGA1 grown using saponified palm kernel oil as carbon source and ammonium as the limiting nutrient. The growth of the micro-organism is well-described using Tessier-type model which takes into account the inhibitory effect of ammonium at high concentrations. The ammonium consumption rate by the cells is related in proportion to the rate of growth. The intracellular production of medium-chain-length poly-(3-hydroxyalkanoates) (PHAMCL) by P. putida PGA1 cells is reasonably modeled by the modified Luedeking-Piret kinetics, which incorporate a function of product synthesis inhibition (or reduction) by …

Autologous Chondrocyte Transplantation In The Repair Of Full-Thickness Focal Cartilage Damage In Rabbits, Tunku Kamarul, L. Selvaratnam, T. Masjuddin, S. Ab-Rahim, C. Ng, K.Y. Chan, T.S.T. Ahmad

Research Publications (2006 to 2010)

PURPOSE:To compare the efficacy of autologous chondrocyte transplantation (ACT) versus non-operative measures for cartilage repair in rabbits. METHODS:Nine New Zealand white rabbits were used. Identical focal defects were created in the articular cartilage of both knees. One month later, the right knee was repaired via ACT, while the left knee was left untreated (control group). The quality of cartilage tissues in both knees was compared 3 months later, according to the quantitative analysis of glycosaminoglycan (GAG) in the cartilage and macroscopic examination of histology using the Brittberg/International Cartilage Research Society (ICRS) score. RESULTS:Microscopic examination showed enhanced regeneration following ACT repair. …

Discordance In The Paraoxonase (Pon1)-192qr Polymorphism, R. Poh, S. Muniandy

Research Publications (2006 to 2010)

Paraoxonase (PON1) has been implicated to have a cardioprotective role, due to its physical attachment with high-density lipoprotein. PON1192QR is a variation of the PON1 gene, the R allele being a risk factor for cardiovascular disease. Kinetic studies resulting in a plot of paraoxon versus diazoxon hydrolysis rates may be used to accurately predict PON1192 geno-type. In this study, paraoxonase and diazoxonase activities in plasma were measured spec-trophotometrically using plasma while PCR-based PON1192 genotyping was performed us-ing polymerase chain reaction followed by restriction digestion. The two-substrate assay-derived genotypes were cross-referred with those determined by PCR-based genotyping. When results did not …

Dragonflies On The Islands In The Straits Of Malacca, Yusoff Norma-Rashid, Mohd Sofian-Azirun, R. Ramli, R. Hashim

Research Publications (2006 to 2010)

The odonate fauna of Pulau Perak, Pulau Jarak and some islands of the Sembilan group of islands were surveyed. Although no endemics were found, fifteen species, all first records for the islands were documented. Various biological aspects of the dragonfly such as population, distribution and relative abundance were discussed. Morphometric aspects were also studied and possible explanations for the attributed differences between the island populations of Pulau Perak and Pulau Lalang are proposed.

Quantification Of Zonula Occludens-1 Mrna Expression In Cultured Bovine Retinal Endothelial Cells, Using Sybr Green Real-Time Pcr, S. Muniandy, K.C. Yaiw, J.B. Chook, A.V. Rayappan, K.T. Wong, I.S. Ismail, R. Qvist

Research Publications (2006 to 2010)

A simple and rapid real-time reverse transcription-PCR (RT-PCR) technique using SYBR green fluorescence was developed to quantify Zonula Occludens-1 (ZO-1) mRNA expression in histamine-treated cultured bovine retinal microvessel endothelial cells. There was no difference between the quantity of ZO-1 mRNA expression in histamine-treated (10(-7)M to 10(-9) M) and untreated bovine retinal endothelial cells at high magnesium levels. Immunohistochemistry staining and western blot assay unequivocally demonstrated the approximately similar levels of ZO-1 protein in both histamine-treated and non-treated endothelial cells at high magnesium concentrations.

Finite Element Analysis Of An Automated Rapid Maxillary Expander (Arme), F.A. Zabir, A.S. Abdullah, Noor Azuan Abu Osman, Z. Radzi, N.A. Yahya, Noor Hayaty Abu Kasim

Research Publications (2006 to 2010)

An Automated Rapid Maxillary Expander (ARME), is a specially designed orthodontic appliance to overcome the shortcomings imposed by the traditional butterfly expansion appliance. It operates by automatically widening the maxilla (upper jaw) by expanding the midpalatal suture [1]. This procedure is not feasible after late teenage years due to more rigid facial skeleton features. According to the study by Cozzani [2], the activation of the device could produce 0.25 mm of expansion per day. Over the period of 2 weeks, the expansion produced is up to 7 mm in total [1]. For this study, we explored the mechanism involved in …

Morehead State University 2008-09 Men's Golf, Morehead State University. Office Of Athletics.

Morehead State Sports Team Programs

The 2008-2009 official media guide of the Morehead State University men's golf team.

Morehead State 2008 Eagle Football, Morehead State University. Office Of Athletics.

Morehead State Sports Team Programs

The 2008 official media guide of the Morehead State University foottball team.

Kelo-Parents Involved And The Spatialization Of Color (Blindness) In The Berman-Brown Metropolitan Heterotopia, Tom I. Romero

Sturm College of Law: Faculty Scholarship

The article uses the 1954 eminent domain and school integration decisions in Berman v. Parker and Brown v. Board of Education and the more recent Kelo v. New London and Parents Involved in Community Schools v. Seattle School District cases to examine the rise of the multiracial metropolis in the United States. By linking and concomitantly exploring the United States Supreme Court’s eminent domain and school desegregation decisions, the article offers a fundamentally new approach to understanding the consequence and import of these decisions in managing property and race relations in the contemporary United States.

Analytical emphasis on the period …

A Biotic Survey And Inventory Of The Dynastine Scarab Beetles Of Mesoamerica, North America, And The West Indies: Review Of A Long-Term, Multicountry Project, Brett C. Ratcliffe, Ronald D. Cave

University of Nebraska State Museum: Entomology Papers

This biotic inventory will document the species-level diversity of dynastine scarabs, their spatial and temporal distributions, ecological preferences, and biology. The long term, multi-country research project explores a biotically megadiverse region that is seriously at risk from deforestation, environmental homogenization, invasive species, and urban sprawl. Objectives of this survey are: 1) to understand the biodiversity of dynastine scarab beetles in Mesoamerica; 2) disseminate this information in print and electronic forms; 3) train students, parataxonomists, and collection managers in the study area about dynastine taxonomy and identification, care of collections, dissemination of information, and conservation; and 4) assemble authoritatively identified voucher …

G08-1823 Barley Yellow Dwarf Of Wheat, Barley, And Oats, Stephen N. Wegulo, Gary L. Hein, Roy C. French

University of Nebraska-Lincoln Extension: Historical Materials

The symptom's, impacts and management of Barley Yellow Dwarf (BYD), a virus disease affecting small grains, are discussed in this 2008 NebGuide. This information is based on observations of BYD in Nebraska wheat and oat fields in 2007 and on published research conducted elsewhere.

2007-2008 Annual Report

Lancaster County, Nebraska Extension: Reports

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Trends In Selenium Utilization In Marine Microbial World Revealed Through The Analysis Of The Global Ocean Sampling (Gos) Project, Yan Zhang, Vadim N. Gladyshev

Vadim Gladyshev Publications

Selenium is an important trace element that occurs in proteins in the form of selenocysteine (Sec) and in tRNAs in the form of selenouridine. Recent large-scale metagenomics projects provide an opportunity for understanding global trends in trace element utilization. Herein, we characterized the selenoproteome of the microbial marine community derived from the Global Ocean Sampling (GOS) expedition. More than 3,600 selenoprotein gene sequences belonging to 58 protein families were detected, including sequences representing 7 newly identified selenoprotein families, such as homologs of ferredoxin– thioredoxin reductase and serine protease. In addition, a new eukaryotic selenoprotein family, thiol reductase GILT, was identified. …

Rapid Enzyme-Linked Immunosorbent Assay For Detection Of The Algal Toxin Domoic Acid, R Wayne Litaker, Thomas N. Stewart, Bich-Thuy L. Eberhart, John C. Wekell, Vera L. Trainer, Raphael M. Kudela, Peter E. Miller, Alice Roberts, Cassandra Hertz, Tyler A. Johnson, Greg Frankfurter, G. Jason Smith, Astrid Schnetzer, Joe Schumacker, Jonnette L. Bastian, Anthony Odell, Patrick Gentien, Dominique Le Gal, D Ransom Hardison, Patricia A. Tester

Natural Sciences and Mathematics | Faculty Scholarship

Domoic acid (DA) is a potent toxin produced by bloom-forming phytoplankton in the genus Pseudo-nitzschia, which is responsible for causing amnesic shellfish poisoning (ASP) in humans. ASP symptoms include vomiting, diarrhea, and in more severe cases confusion, loss of memory, disorientation, and even coma or death. This paper describes the development and validation of a rapid, sensitive, enzyme linked immunosorbent assay test kit for detecting DA using a monoclonal antibody. The assay gives equivalent results to those obtained using standard high performance liquid chromatography, fluorenylmethoxycarbonyl high performance liquid chromatography, or liquid chromatography—mass spectrometry methods. It has a linear range …

Welcome To The (Impenetrable) Jungle: Massachusetts V. Epa, The Clean Air Act And The Common Law Of Public Nuisance, P. Leigh Bausinger

Villanova Law Review (1956 - )

No abstract provided.

Fan Letter, Spring/Summer 2008, Illinois State University, College Of Fine Arts

FAN Letter

Newsletter of the College of Fine Arts

Mass Spectrometry Of Dendrimer Metal Complexes, Jason David Batchelor

Theses, Dissertations and Capstones

Dendrimers are hyper-branched macromolecules with unique properties. They form using a “cascade-like” synthesis involving repetitive reaction steps. These steps are controlled, resulting in monodisperse dendrimers. Synthesis is divergent, core to exterior, or convergent, exterior to core. Dendrimers are created from various molecules with numerous functional properties. They are used as catalysts, diagnostic agents, and others.

This thesis studies 1st generation poly(propylene) imine, consisting of a diaminobutane core with 4 propyl amine branches. Fragmentation of protonated and metal (Co, Ni, Cu and Zn) complexed dendrimers was analyzed by ion-trap mass spectrometry. Two isotopically labeled dendrimers were synthesized for neutral loss identification …

The Aridity Of Grace: Community And Ecofeminism In Barbara Kingsolver's Animal Dreams And Prodigal Summer, Richard M. Magee

English Faculty Publications

In both Animal Dreams and her later novel Prodigal Summer, Kingsolver constructs narratives of community inhabited by characters with a vivid awareness of the natural world and the threats to that world; furthermore, both novels feature strong female characters who long for a more harmonious life within nature. The novels develop and present forthright ecofeminist themes, with the women in the texts representing ideals of ecologically sensitive living who seek to educate their communities about threats to the environment and the defenses against those threats.

Kingsolver's ecofeminist vision, however, is frequently complicated and contradictory; just as the desert landscape …

Terrestrial Habitat Use Of Marbled Salamanders Ambystoma Opacum: A Site Specific Approach, Frank Piccininni

Theses, Dissertations and Capstones

Habitat destruction, fragmentation and degradation have contributed heavily to the decline of amphibian populations such as Marbled Salamanders (Ambystoma opacum). Often, ambystomatid water resources are conserved without consideration for the equally important terrestrial habitat. This is partly due to a lack of information regarding the relationship between ecological succession, plant community composition, microhabitat and salamander abundance. Three sampling transects consisting of drift fence arrays, vegetation assessments and microhabitat surveys were extended 100 m into the terrestrial habitat surrounding a seasonal wetland at Beech Fork State Park in Wayne County, West Virginia. Principal components analysis was used to identify …

Soviet Images Of Jehovah In The 1920s, Robert Weinberg

History Faculty Works

No abstract provided.

The Caenorhabditis Globin Gene Family Reveals Extensive Nematode-Specific Radiation And Diversification, David Hoogewijs, Sasha De Henau, Sylvia Dewilde, Luc Moens, Marjolein Couvreur, Gaetan Borgonie, Serge N. Vinogradov, Scott W. Roy, Jacques R. Vanfleteren

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Globin isoforms with variant properties and functions have been found in the pseudocoel, body wall and cuticle of various nematode species and even in the eyespots of the insect-parasite Mermis nigrescens. In fact, much higher levels of complexity exist, as shown by recent whole genome analysis studies. In silico analysis of the genome of Caenorhabditis elegans revealed an unexpectedly high number of globin genes featuring a remarkable diversity in gene structure, amino acid sequence and expression profiles.

Results

In the present study we have analyzed whole genomic data from C. briggsae, C. remanei, Pristionchus pacificus and Brugia malayi …

A Phylogenomic Profile Of Hemerythrins, The Nonheme Diiron Binding Respiratory Proteins, Xavier Bailly, Stefano Vanin, Christine Chabasse, Kenji Mizuguchi, Serge N. Vinogradov

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Hemerythrins, are the non-heme, diiron binding respiratory proteins of brachiopods, priapulids and sipunculans; they are also found in annelids and bacteria, where their functions have not been fully elucidated.

Results

A search for putative Hrs in the genomes of 43 archaea, 444 bacteria and 135 eukaryotes, revealed their presence in 3 archaea, 118 bacteria, several fungi, one apicomplexan, a heterolobosan, a cnidarian and several annelids. About a fourth of the Hr sequences were identified as N- or C-terminal domains of chimeric, chemotactic gene regulators. The function of the remaining single domain bacterial Hrs remains to be determined. In …

Relation Between Nodule Size And 18F-Fdg-Pet Suv For Malignant And Benign Pulmonary Nodules., Majid Khalaf, Hani Abdel-Nabi, John Baker, Yiping Shao, Dominick Lamonica, Jayakumari Gona

Wayne State University Associated BioMed Central Scholarship

Abstract

The most common semiquantitative method of evaluation of pulmonary lesions using ¹⁸F-FDG PET is FDG standardized uptake value (SUV). An SUV cutoff of 2.5 or greater has been used to differentiate between benign and malignant nodules. The goal of our study was to investigate the correlation between the size of pulmonary nodules and the SUV for benign as well as for malignant nodules.

Methods

Retrospectively, 173 patients were selected from 420 referrals for evaluation of pulmonary lesions. All patients selected had a positive CT and PET scans and histopathology biopsy. A linear regression equation was fitted to a …

Preclinical Studies Of Apogossypolone: A New Nonpeptidic Pan Small-Molecule Inhibitor Of Bcl-2, Bcl-XL And Mcl-1 Proteins In Follicular Small Cleaved Cell Lymphoma Model, Alan A. Arnold, Amro Aboukameel, Jianyong Chen, Dajun Yang, Shaomeng Wang, Ayad Al-Katib, Ramzi M. Mohammad

Wayne State University Associated BioMed Central Scholarship

Abstract

Elevated expression of anti-apoptotic Bcl-2 family proteins have been linked to a poor survival rate of patients with Follicular Lymphoma (FL). This prompted us to evaluate a very potent non-peptidic Small-Molecule Inhibitor (SMI) targeting Bcl-2 family proteins, Apogossypolone (ApoG2) using follicular small cleaved cell lymphoma cell line (WSU-FSCCL) and cell isolated from lymphoma patients. ApoG2 inhibited the growth of WSU-FSCCL significantly with a 50% growth inhibition of cells (IC₅₀) of 109 nM and decreased cell number of fresh lymphoma cells. ApoG2 activated caspases-9, -3, and -8, and the cleavage of Poly (ADP-ribose) polymerase (PARP) and Apoptosis Inducing …

Gene Expression Profiles In Primary Pancreatic Tumors And Metastatic Lesions Of Ela-C-Myc Transgenic Mice, Archana Thakur, Aliccia Bollig, Jiusheng Wu, Dezhong J. Liao

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

Pancreatic carcinoma usually is a fatal disease with no cure, mainly due to its invasion and metastasis prior to diagnosis. We analyzed the gene expression profiles of paired primary pancreatic tumors and metastatic lesions from Ela-c-myc transgenic mice in order to identify genes that may be involved in the pancreatic cancer progression. Differentially expressed selected genes were verified by semi-quantitative and quantitative RT-PCR. To further evaluate the relevance of some of the selected differentially expressed genes, we investigated their expression pattern in human pancreatic cancer cell lines with high and low metastatic potentials.

Results

Data indicate that genes …

Fostering Preservice Teacher Identity In Science Through A Student-Selected Project, D.J. Wink, J. Ellefson, M. Nishimura, D. Perry, S. Wenzel, Jeong Hwang Choe

Faculty Publications & Research

This article addresses the problem of authentic student engagement in the science classroom by incorporating a semester long research and writing assignment that enables students to investigate scientific topics related to strong personal, career, or health interests.

Annual Report 2008 Australian College Of Educators, Australian College Of Educators

Australian College of Educators - National

No abstract provided.

Rewarding Research, Jay Cox

Syracuse University Magazine

No abstract provided.

Horace Mann's Vision In Action: Bridgewater Normal School's Female Teachers, Casey Rekowski

Undergraduate Review

Through a close reading of Horace Mann’s archival material including official documents and speeches, this paper argues that Horace Mann expanded women’s social roles in the nineteenth century. Whether he intended such an outcome or not, Horace Mann’s agenda to improve common schools increased American women’s educational and professional opportunities. Drawing upon the popular ideology of the day, Mann articulated a detailed rationale for placing women, as “natural teachers” at the center of public education in Massachusetts when he became the first secretary of the Massachusetts Board of Education in 1837. This can best be seen through his annual reports …

A Joyful Soul: A Case History Of A Bridgewater Nursing Home Resident, Heidi Woofenden

Undergraduate Review

No abstract provided.