Digital Commons Network^™

Comparative Metabolite Profiling Of Carboxylic Acids In Rat Urine By Ce-Esi Ms/Ms Through Positively Pre-Charged And 2H-Coded Derivatization, Wen-Chu Yang, Fred E. Regnier, Jiri Adamec

Department of Biochemistry: Faculty Publications

A new approach for the selective comparative metabolite profiling of carboxylic acids in rat urine was established using capillary electrophoresis-mass spectrometry (CE-MS) and a method for positively pre-charged and ²H-coded derivatization. Novel derivatizing reagents, N-alkyl-4-

aminomethylpyridinum iodide (alkyl=butyl, butyl-d9 or hexyl), containing quaternary amine and

stable isotope atoms (deuterium), were introduced for the derivatization of carboxylic acids. CE

separation in positive polarity showed high reproducibility (0.99 –1.32% RSD of migration time)

and eliminated problems with capillary coating known in CE-MS anion analyses. Essentially

complete ionization and increased hydrophobicity after the derivatization also enhanced MS

detection sensitivity (e.g. formic …

Regulation Of Aromatic Alcohol Production In Candida Albicans, Suman Ghosh, Bessie W. Kebaara, Audrey L. Atkin, Kenneth W. Nickerson

School of Biological Sciences: Faculty Publications

Colonization by the fungal pathogen Candida albicans varies significantly, depending upon the pH and availability of oxygen. Because of our interest in extracellular molecules as potential quorum-sensing molecules, we examined the physiological conditions which regulate the production of the aromatic alcohols, i.e., phenethyl alcohol, tyrosol, and tryptophol. The production of these fusel oils has been well studied for Saccharomyces cerevisiae. Our data show that aromatic alcohol yields for C. albicans are determined by growth conditions. These conditions include the availability of aromatic amino acids, the pH, oxygen levels, and the presence of ammonium salts. For example, for wild-type C. …

Pea Aphid, Jennifer A. Brisson, Gregory K. Davis

School of Biological Sciences: Faculty Publications

Aphids have long been of interest because of their complex life cycles, environmentally induced morphologies, and importance to agriculture. However, only recently has the resulting wealth of ecological and population genetic data begun to be supplemented by genomic and genetic mapping approaches. In 2004, the International Aphid Genomics Consortium, a collaboration of aphid researchers, chose to establish the pea aphid, Acyrthosiphon pisum, as the aphid of choice for the development of genomic resources. Here we introduce the pea aphid and discuss a number of biological questions for which the species is well suited. We then review previous mapping and quantitative …

The Genome Sequence Of The Metal-Mobilizing, Extremely Thermoacidophilic Archaeon Metallosphaera Sedula Provides Insights Into Bioleaching-Associated Metabolism, Kathryne S. Auernik, Yukari Maezato, Paul H. Blum, Robert M. Kelly

School of Biological Sciences: Faculty Publications

Despite their taxonomic description, not all members of the order Sulfolobales are capable of oxidizing

reduced sulfur species, which, in addition to iron oxidation, is a desirable trait of biomining microorganisms.

However, the complete genome sequence of the extremely thermoacidophilic archaeon Metallosphaera sedula

DSM 5348 (2.2 Mb, _2,300 open reading frames [ORFs]) provides insights into biologically catalyzed metal

sulfide oxidation. Comparative genomics was used to identify pathways and proteins involved (directly or

indirectly) with bioleaching. As expected, the M. sedula genome contains genes related to autotrophic carbon

fixation, metal tolerance, and adhesion. Also, terminal oxidase cluster organization indicates the presence …

Ecological Genomics: Understanding Gene And Genome Function In The Natural Environment, M. C. Ungerer, L. C. Johnson, M. A. Herman

School of Biological Sciences: Faculty Publications

The field of ecological genomics seeks to understand the genetic mechanisms underlying responses of organisms to their natural environments. This is being achieved through the application of functional genomic approaches to identify and characterize genes with ecological and evolutionary relevance. By its very nature, ecological genomics is an interdisciplinary field. In this review, we consider the significance of this new area of study from both an ecological and genomic perspective using examples from the recent literature. We submit that by considering more fully an ecological context, researchers may gain additional insights into the underlying genetic basis of ecologically relevant phenotypic …

Presidential Address: Nothing Succeeds Like Excess, Steven A. Nadler

Harold W. Manter Laboratory of Parasitology: Faculty Publications

To the members of the American Society of Parasitologists, I offer my appreciation for the opportunity to serve as your president. When I was notified of my successful election as an officer, it brought to mind that famous remark by Adlai Stevenson who said, ‘‘In America, anybody can be president. That’s one of the risks you take.’’ So, what I initially perceived as somewhat of a risky venture, proved to be a more valuable and interesting experience than I anticipated. Let’s hope that my presidential address has a similar outcome.

Tricarboxylic Acid Cycle-Dependent Regulation Of Staphylococcus Epidermidis Polysaccharide Intercellular Adhesin Synthesis, Marat R. Sadykov, Michael E. Olson, Steven M. Halouska, Yefei Zhu, Paul D. Fey, Robert Powers, Greg A. Somerville

Robert Powers Publications

Staphylococcus epidermidis is a major nosocomial pathogen primarily infecting immunocompromised individuals or those with implanted biomaterials (e.g., catheters). Biomaterial-associated infections often involve the formation of a biofilm on the surface of the medical device. In S. epidermidis, polysaccharide intercellular adhesin (PIA) is an important mediator of biofilm formation and pathogenesis. Synthesis of PIA is regulated by at least three DNA binding proteins (IcaR, SarA, and σ^B) and several environmental and nutritional conditions. Previously, we observed the environmental conditions that increased PIA synthesis decreased tricarboxylic acid (TCA) cycle activity. In this study, S. epidermidis TCA cycle mutants were …

Factors Affecting Child Mortality In Saudi Arabia, Yagob Y. Al-Mazrou, Nasser A. Alhamdan, Abduelelah I. Alkotobi, Osman M. Nour, Mohamed A. Farag

Saudi Medical Journal

OBJECTIVE: To estimate levels and trends, gender differentials, effect of modes of living, regional differentials, and the effect of parental educational on infant and child mortality. METHODS: A nationwide stratified random sample was used to estimate levels, trends and differentials of infant and child mortality in Saudi Arabia. The study was executed during the period February 2006 to June 2006 and covered all the 20 health regions of Saudi Arabia. The randomly selected sample was limited to ever married Saudi women in the reproductive age group (15-49 years). RESULTS: The findings show a continuous and considerable infant and child mortality …

The Prevalence Of Malnutrition Among High And Low Altitude Preschool Children Of Southwestern Saudi Arabia, Fahaid H. Al-Hashem

Saudi Medical Journal

OBJECTIVE: To assess the current status of protein energy malnutrition (PEM) in the high and low altitude preschool children aged 12-71 months. METHODS: A cross-sectional study conducted during the year of 2003 and involved 572 and 469 preschool children of Southwestern Saudi Arabia born and living permanently at high and low altitude areas. Anthropometric measurements were carried out to assess the prevalence of PEM using 3 indicators such as underweight, wasting and stunting following World Health Organization standards. Prevalence differences were examined by age, gender, altitude, and parental socioeconomic status. RESULTS: The prevalence of the 3 types of PEM was …

Fumonisin Level In Corn-Based Food And Feed From Linxian County, A High-Risk Area For Esophageal Cancer In China, Jiansheng Wang, Ying Zhou, Wanbo Liu, Xiangcheng Zhu, Liangcheng Du, Qiaomei Wang

Liangcheng Du Publications

The level of mycotoxin fumonisins in corn-based food and feed collected from Linxian County, a high-risk area for esophageal cancer in China, has been analyzed using high-performance liquid chromatographic coupled with evaporative laser scattering detector (HPLC-ELSD). A total of 104 corn kernel samples were obtained from local households, granaries, wholesale markets (central markets), and retail markets (stores and supermarkets). Fumonisin B1 (FB1) was detected in the samples from households, granaries, central markets, and stores, with a positive rate of 61.5%, 50%, 33.3%, and 17%, respectively. No fumonisin was detected in samples from the supermarket. The highest FB1 levels (0.30–3.20 μg/ …

Mechanisms Of Hemoglobin Adaptation To High Altitude Hypoxia, Jay F. Storz, Hideaki Moriyama

Hideaki Moriyama Publications

Evidence from a number of vertebrate taxa suggests that modifications of hemoglobin (Hb) function may often play a key role in mediating an adaptive response to high altitude hypoxia. The respiratory functions of Hb are a product of the protein’s intrinsic O₂-binding affinity and its interactions with allosteric effectors such as protons, chloride ions, CO₂, and organic phosphates. Here we review several case studies involving high altitude vertebrates where it has been possible to identify specific mechanisms of Hb adaptation to hypoxia. In addition to comparative studies of Hbs from diverse animal species, functional studies of …

Mechanisms Of Hemoglobin Adaptation To High Altitude Hypoxia, Jay F. Storz, Hideaki Moriyama

Hideaki Moriyama Publications

Evidence from a number of vertebrate taxa suggests that modifications of hemoglobin (Hb) function may often play a key role in mediating an adaptive response to high altitude hypoxia. The respiratory functions of Hb are a product of the protein’s intrinsic O₂-binding affinity and its interactions with allosteric effectors such as protons, chloride ions, CO₂, and organic phosphates. Here we review several case studies involving high altitude vertebrates where it has been possible to identify specific mechanisms of Hb adaptation to hypoxia. In addition to comparative studies of Hbs from diverse animal species, functional studies of …

Parasite Pathoecology Of Salmon Pueblo And Other Chacoan Great Houses: The Healthiest And Wormiest Ancestral Puebloans, Karl Reinhard

Karl Reinhard Publications

Two fields of paleopathological investigation originated in the Southwest. Archaeoparasitology is the study of ancient parasite infection (Reinhard 1990, 1992b). It includes comparisons between time periods of single societies as well as comparisons of parasitism between different, contemporaneous cultures. For example, Fry (1980) compared Fremont and Anasazi parasitism, and also Archaic hunter-gatherer and ancestral Pueblo parasitism. All of these studies fall into the definition of archaeoparasitology.

By contrast, pathoecology is the reconstruction of relationships among behavior, environment, and disease organisms in the development of illness (Martinson et al. 2003; ReinhardandBuikstra2003; Reinhardet al. 2003; Santoro et al. 2003).1his field developed from …

Pathoecology And The Future Of Coprolite Studies In Bioarchaeology, Karl J. Reinhard, Vaughn M. Bryant Jr.

Karl Reinhard Publications

Human coprolites currently provide an expanding array of information about the diet, health, and ecology of prehistoric people in the Southwest, but for many years coprolites were not recognized or preserved, or they were not considered important and thus were not saved (Bryant and Dean 2006). With the expansion of archaeological field work during the last half of the twentieth century archaeologists have increasingly explored the "complete" potentials of sites, including the collection and analysis of geomorphologic, botanical, and faunal data. In some ideal habitats (e.g., very dry or frozen) this includes exploring the scientific potential of human coprolite studies. …

Pathoecology Of Two Ancestral Pueblo Villages, Karl Reinhard

Karl Reinhard Publications

Pathoecology is the study of the biotic, abiotic, and cultural environments of disease (Martinson et al. 2003). A parasitic infection is the result of the pathoecological interaction of host behavior, parasite life cycle, the environment in which both life forms live, the nutritional status of the host, and host physiological responses to all of these factors. Parasites contribute to anemia in many ways. Some, such as hookworm, actually consume blood and cause iron loss through their activities. For other parasites, symptoms such as profuse diarrhea reduce intestinal absorption of nutrients. Others, such as certain fish tapeworms, actually compete for absorption …

The New Empirical Biopolitics, John R. Alford, John R. Hibbing

Department of Political Science: Faculty Publications

Political science traditionally has either ignored biology in favor of purely environmental explanations for political phenomena or merely ruminated about the likely role of biology, leaving data-based research on biopolitics in dangerously short supply. Currently, attention to the apparent genetic basis for political and social orientations holds the greatest promise of advancing empirical biopolitics. Thus, in this essay, we orient behavior genetics research in the larger framework of biology and politics, confront its normative implications, describe the techniques involved, assess the strengths and weaknesses of commonly employed data and procedures, and describe the next steps in this research stream. Because …

Avoidance Of Hydrolyzed Casein By Mice, Kristin L. Field, Bruce A. Kimball, Julie A. Mennella, Gary K. Beauchamp, Alexander A. Bachmanov

United States Department of Agriculture Wildlife Services: Staff Publications

When casein, a milk protein, is hydrolyzed, it renders human foods that contain it (e.g., hypoallergenic infant formula, cheeses) distasteful to many people. This rejection of hydrolyzed casein (HC)-containing foods has recently been found to also occur in a non-human species (deer, Odocoileus spp.). Identifying other animals that avoid HC would facilitate understanding how and why HC-containing food is often rejected. This study determined whether HC-containing food is avoided by Mus musculus and whether consumption patterns were sensitive to testing conditions, specifically food form (powder, pellet or dough) and food access (ad libitum or 1.5 h/day following 6 h …

Winter Cover Crops And Vinegar For Early-Season Weed Control In Sustainable Cotton, P. J. Moran, S. M. Greenberg

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Weeds may be suppressed by winter cover crops and the use of organic herbicides such as vinegar. Black oat (Avena strigosa) and hairy vetch (Vicia villosa) winter cover crops were planted for 2 years as part of a sustainable production system for cotton in the Lower Rio Grande Valley of Texas, and were till-killed each spring prior to cotton planting. Palmer amaranth (Amaranthus palmeri), common purslane (Portulaca oleracea), and sunflower (Helianthus annuus) were frequently-encountered winter and spring weeds. Both cover crops controlled winter weeds as well as did winter tillage …

Babbling, Chewing, And Sucking: Oromandibular Coordination At 9 Months, Roger W. Steeve, Christopher A. Moore, Jordan R. Green, Kevin J. Reilly, Jacki Ruark Mcmurtrey

Department of Special Education and Communication Disorders: Faculty Publications

Purpose: The ontogeny of mandibular control is important for understanding the general neurophysiologic development for speech and alimentary behaviors. Prior investigations suggest that mandibular control is organized distinctively across speech and nonspeech tasks in 15-month-olds and adults and that, with development, these extant forms of motor control primarily undergo refinement and rescaling. The present investigation was designed to evaluate whether these coordinative infrastructures for alimentary behaviors and speech are evident during the earliest period of their co-occurrence.
Method: Electromyographic (EMG) signals were obtained from the mandibular muscle groups of 15 typically developing 9-month-old children during sucking, chewing, and speech.
Results: …

Respiratory Treatment History Predicts Suck Pattern Stability In Preterm Infants, Meredith Poore, Steven M. Barlow, Jingyan Wang, Mereditch Estep, Jaehoon Lee

Department of Special Education and Communication Disorders: Faculty Publications

Sensory deprivation and motor restriction associated with extensive oxygen therapy may lead to poor oromotor control in preterm infants. Non-nutritive suck is one of the first complex oromotor behaviors infants perform. This study determined the spatiotemporal variability of non-nutritive suck (NNS) pressure trajectories in three preterm groups with differing oxygen histories—one control group with minimal or no O₂ therapy, and two Respiratory Distress Syndrome (RDS) groups with either a mild/moderate (RDS1) or moderate/severe (RDS2) O₂ history. The Non-nutritive Suck Spatiotemporal Index (NNS STI) quantifies spatial and temporal variability across kinematic trajectories, and was calculated from digital representations of …

Influence Of Plankton Mercury Dynamics And Trophic Pathways On Mercury Concentrations Of Top Predator Fish Of A Mining-Impacted Reservoir, A. Robin Stewart, Michael K. Saiki, James S. Kuwabara, Charles N. Alpers, Mark Marvin-Dipasquale, David P. Krabbenhoft

United States Geological Survey: Staff Publications

Physical and biogeochemical characteristics of the aquatic environment that affect growth dynamics of phytoplankton and the zooplankton communities that depend on them may also affect uptake of methylmercury (MeHg) into the pelagic food web of oligotrophic reservoirs. We evaluated changes in the quality and quantity of suspended particulate material, zooplankton taxonomy, and MeHg concentrations coincident with seasonal changes in water storage of a mining-impacted reservoir in northern California, USA. MeHg concentrations in bulk zooplankton increased from 4 ng·g^–1 at low water to 77 ± 6.1 ng·g^–1 at high water and were positively correlated with cladoceran biomass (r = …

Boselaphus Tragocamelus (Artiodactyla: Bovidae), David M. Leslie Jr.

United States Geological Survey: Staff Publications

Boselaphus tragocamelus (Pallas, 1766) is a bovid commonly called the nilgai or blue bull and is Asia’s largest antelope. A sexually dimorphic ungulate of large stature and unique coloration, it is the only species in the genus Boselaphus. It is endemic to peninsular India and small parts of Pakistan and Nepal, has been extirpated from Bangladesh, and has been introduced in the United States (Texas), Mexico, South Africa, and Italy. It prefers open grassland and savannas and locally is a significant agricultural pest in India. It is not of special conservation concern and is well represented in zoos and …

Management And Conservation Note: Conserving Waste Rice For Wintering Waterfowl In The Mississippi Alluvial Valley, Jennifer P. Kross, Richard M. Kaminski, Aaron T. Pearse

United States Geological Survey: Staff Publications

The Mississippi Alluvial Valley (MAV), USA, is an internationally important migration and wintering region for North American waterfowl (Reinecke et al. 1989). The Lower Mississippi Valley Joint Venture (LMVJV) assumes food availability is the primary factor influencing carrying capacity of wintering waterfowl in this region (Reinecke and Loesch 1996). Because much of the MAV was converted from a seasonally flooded bottomland-hardwood ecosystem to a landscape dominated by agriculture, the LMVJV has incorporated estimates of the abundance of agricultural seeds such as rice into habitat conservation plans (Reinecke et al. 1988, Fredrickson et al. 2005). Biologists designed these plans to provide …

Omtvedt Innovation Award Winners, John Owens

John Owens: Speeches and Appearances

It is a wonderful pleasure to have this opportunity today to present, two Omtvedt Innovation Awards to Institute colleagues whose outstanding work is a source of tremendous pride and inspiration for us all.

Minimal Nutrition Intervention With High-Protein/Low-Carbohydrate And Low-Fat, Nutrient-Dense Food Supplement Improves Body Composition And Exercise Benefits In Overweight Adults: A Randomized Controlled Trial, Christopher M. Lockwood, Jordan R. Moon, Sarah E. Tobkin, Ashley A. Walter, Abbie E. Smith, Vincent J. Dalbo, Joel T. Cramer, Jeffrey R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

Background: Exercise and high-protein/reduced-carbohydrate and -fat diets have each been shown separately, or in combination with an energy-restricted diet to improve body composition and health in sedentary, overweight (BMI > 25) adults. The current study, instead, examined the physiological response to 10 weeks of combined aerobic and resistance exercise (EX) versus exercise + minimal nutrition intervention designed to alter the macronutrient profile, in the absence of energy restriction, using a commercially available high-protein/low-carbohydrate and low-fat, nutrient-dense food supplement (EXFS); versus control (CON).

Methods: Thirty-eight previously sedentary, overweight subjects (female = 19; male = 19) were randomly assigned to either CON (n …

Pre-Workout Consumption Of Celsius® Enhances The Benefits Of Chronic Exercise On Body Composition And Cardiorespiratory Fitness, Jeffrey R. Stout, Jordan R. Moon, Sarah E. Tobkin, Christopher M. Lockwood, Abbie E. Smith, Jennifer L. Graef, Kristina L. Kendall, Travis W. Beck, Joel T. Cramer

Department of Nutrition and Health Sciences: Faculty Publications

The functional beverage Celsius®, has recently been shown, after acute and chronic (28 days) consumption, to increase resting metabolism and serum blood markers of lipolysis in healthy, college-aged men and women. The purpose of this study was to examine the combined effects of a 10-week exercise program while consuming Celsius® on body composition and cardiorespiratory fitness changes in sedentary men and women.

Beta-Alanine Supplementation And High-Intensity Interval Training Augments Metabolic Adaptations And Endurance Performance In College-Aged Men, Abbie E. Smith, Ashley A. Walter, Kristina L. Kendall, Jennifer L. Graef, Christopher M. Lockwood, Jordan R. Moon, Travis W. Beck, Joel T. Cramer, Jeffery R. Stout

Department of Nutrition and Health Sciences: Faculty Publications

A randomized, double-blind, placebo-controlled study was conducted to evaluate the effects β-alanine supplementation and high-intensity interval training (HIIT) on endurance performance.

Where's The Kale? Environmental Availability Of Fruits And Vegetables In Two Racially Dissimilar Communities, Edith M. Williams, Bamidele O. Tayo, Beverly Mclean, Ellen Smit, Christopher T. Sempos, Carlos J. Crespo

Faculty Publications

Minority communities across the United States have limited numbers of stores that offer a variety of fruits and vegetables, creating major barriers to good eating habits and nutritional practices among minority groups such as African Americans. Factors like environmental availability of healthy food options have not been fully investigated as possible sources of current cross-population differences in disease. The present study examined whether a predominantly African American neighborhood had disproportionately less availability of fruits and vegetables than a predominantly non-Hispanic White neighborhood. Availability was judged on the bases of the types of stores available in each community and the specific …

Polymorphisms In Methionine Synthase, Methionine Synthase Reductase And Serine Hydroxymethyltransferase, Folate And Alcohol Intake, And Colon Cancer Risk, Susan E. Steck, Temitope O. Keku, Lesley M. Butler, Joseph Galanko, Beri Massa, Robert C. Millikan, Robert S. Sandler

Faculty Publications

Background/Aims - We examined associations among folate and alcohol intake, SNPs in genes involved in one-carbon metabolism and colon cancer risk.

Methods - Colon cancer cases (294 African Americans and 349 whites) were frequency matched to population controls (437 African Americans and 611 whites) by age, race and sex from 33 North Carolina counties from 1996 to 2000. Folate and alcohol intakes were collected by dietary interview. Five SNPs were genotyped using DNA from whole blood: SHMT C1420T; MTRR A66G; MTR A2756G, and the previously-reported MTHFR C677T and MTHFR A1298C. Adjusted odds ratios (OR) and 95% …

Use Of Accelerometers In A Large Field-Based Study Of Children: Protocols, Design Issues, And Effects On Precision, Calum Mattocks, Andy R. Ness, Sam D. Leary, Kate Tilling, Steven N. Blair, Julian Shield, Kevin Deere, Joanne Saunders, Joanne Krikby, George Davey Smith, Jonathan Wells, Nicholas Wareham, John J. Reilly, Chris J. Riddoch

Faculty Publications

Background: Objective methods can improve accuracy of physical activity measurement in field studies but uncertainties remain about their use.

Methods: Children age 11 years from the Avon Longitudinal Study of Parents and Children (ALSPAC), were asked to wear a uni-axial accelerometer (MTI Actigraph) for 7 days.

Results: Of 7159 children who attended for assessment, 5595 (78%) provided valid measures. The reliability coefficient for 3 days of recording was .7 and the power to detect a difference of 0.07 SDs (P ≤ .05) was > 90%. Measures tended to be higher on the first day of recording (17 counts/min; 95% CI, …