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2008

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The Effects Of Nutritional Supplement, Mass Fx™, On Muscular Strength, Body Composition, And Blood Chemistries In Resistance Trained Adult Males, Patrick S. Dib Jan 2008

The Effects Of Nutritional Supplement, Mass Fx™, On Muscular Strength, Body Composition, And Blood Chemistries In Resistance Trained Adult Males, Patrick S. Dib

Browse all Theses and Dissertations

The purpose of this study was to determine the effects of a nutritional supplement, Mass FX, on muscular strength, body composition, and blood chemistries in resistance-trained adult males. Eight subjects, mean age 25 +/- 3.02 years, were randomly assigned to two groups (n=4). Each group was given either Mass FX or a Placebo in a double-blind manner to be taken orally for six weeks (4caps/day regardless of bodyweight). For the duration of the study, both groups were following the same training program and a diet customized to each subject's bodyweight in conjunction to the supplementation. Data were analyzed using Analysis …


Dynamic Characterization Of A Pneumatic Muscle Actuator And Its Application To A Resistive Training Device, Jennifer L. Serres Jan 2008

Dynamic Characterization Of A Pneumatic Muscle Actuator And Its Application To A Resistive Training Device, Jennifer L. Serres

Browse all Theses and Dissertations

Research described in this dissertation expands the use of a biomechanical phenomenological model to a commercially available pneumatic muscle actuator (PMA). Due to the nonlinearities of the device, achieving accurate control is challenging. Experiments have been conducted that define boundaries of operation where linear approximations can be used to describe the dynamics of the PMA. Empirical data presented in this dissertation show that nonlinearities exist more prevalently at higher loads (on average >70% contractile force value). When the PMA is under high loads, low displacement occurs. Therefore, these regions are of less interest to design engineers. Once conditions of nonlinearities …


Availability And Toxicity Of Nickel To Lotic Periphyton And Macroinvertebrates, Stephanie Marie Bessom Jan 2008

Availability And Toxicity Of Nickel To Lotic Periphyton And Macroinvertebrates, Stephanie Marie Bessom

Browse all Theses and Dissertations

There is a lack of information regarding the bioaccumulation and toxicity of nickel (Ni) to lotic ecosystems. The objective of this study was to investigate the source, uptake, and toxicity of Ni by periphyton and macroinvertebrates (Hyalella azteca and Daphnia magna) in simulated stream ecosystems that included sediment and water. Periphyton were exposed to two types of sediment, a low-organic sandy mixture and a high organic silt, treated with varying levels of Ni. After 96 h, Ni levels in overlying water and periphyton were related to those in sediments, although differences existed between sediment types. Tests with 64Ni added to …


Automated Analysis Of Metacarpal Cortical Thickness In Serial Hand Radiographs, Aarti Raheja Jan 2008

Automated Analysis Of Metacarpal Cortical Thickness In Serial Hand Radiographs, Aarti Raheja

Browse all Theses and Dissertations

To understand the roles of various genes that influence skeletal bone accumulation and loss, accurate measurement of bone mineralization is needed. However, it is a challenging task to accurately assess bone growth over a person's lifetime. Traditionally, manual analysis of hand radiographs has been used to quantify bone growth, but these measurements are tedious and may be impractical for a large-scale growth study. The aim of this project was to develop a tool to automate the measurement of metacarpal cortical bone thickness in standard hand-wrist radiographs of humans aged 3 months to 70+ years that would be more accurate, precise …


In Defense Of The Indian Child Welfare Act In Aggravated Circumstances, C. Eric Davis Jan 2008

In Defense Of The Indian Child Welfare Act In Aggravated Circumstances, C. Eric Davis

Michigan Journal of Race and Law

The Indian Child Welfare Act (ICWA) affords various protections to Indian families throughout child welfare proceedings. Among them is the duty imposed upon the state to provide rehabilitative services to families prior to the outplacement of an Indian child, or termination of parental rights. An analogous provision for non-Indians in the Adoption and Safe Families Act (ASFA) excuses rehabilitative services in "aggravated circumstances" of child abuse. The ICWA contains no such exception, and that absence has been controversial. In 2002, the Alaska Supreme Court applied ASFA's aggravated circumstances exception to the ICWA, thereby excusing services when a father severely abused …


Jules Verne In English: A Bibliography Of Modern Editions And Scholarly Studies, Arthur B. Evans Jan 2008

Jules Verne In English: A Bibliography Of Modern Editions And Scholarly Studies, Arthur B. Evans

Global Language Studies Faculty publications

No abstract provided.


Regional Differences And Associations With Obesity-Related Factors In Overweight And Obese U.S. Southern Elderly People, Akemi Sakamoto Jan 2008

Regional Differences And Associations With Obesity-Related Factors In Overweight And Obese U.S. Southern Elderly People, Akemi Sakamoto

University of Kentucky Master's Theses

The growing prevalence of overweight and obesity among United States (U.S.) elderly people today is a health concern. Higher incidences of obesity and obesity-related health conditions and mortality exist in the southern area of the U.S. Understanding obesity in relation to obesity-related factors in this population is crucial. The purpose of this study was to identify regional differences and associations between obesity and obesity-related factors in Southern U.S. elderly people, as defined by the U.S. Census Bureau, using data from the 2005 Behavioral Risk Factor Surveillance System (BRFSS), an existing telephone health survey administered by the Centers for Disease Control …


Computer Literacy, Access And Use Of Technology In The Family And Consumer Sciences Classroom, Dana Renee Jenkins Jan 2008

Computer Literacy, Access And Use Of Technology In The Family And Consumer Sciences Classroom, Dana Renee Jenkins

University of Kentucky Master's Theses

For years, schools across the nation have been joining the technology revolution. Today, students have at least some form of technology available to them in school (Roblyer, Castine, andamp; King, 1993; Croxall andamp; Cummings, 2000). This trend is not likely to change, so there is an increasing need for teachers who are literate in the use of the various types of technology. The purpose of this study was to determine if a relationship exists between computer literacy and use of technology, as well as if a relationship exists between teachers access to technology and their use of technology in Family …


Comparison Of Nutritional Intake Of Home School Children And Public School Children: A Comparison Study, Stephen D. Perry Jan 2008

Comparison Of Nutritional Intake Of Home School Children And Public School Children: A Comparison Study, Stephen D. Perry

University of Kentucky Master's Theses

Purpose: To compare selected food /nutrient consumption between families that educate their children at home with those that educate their children in the public school system.

Methods: The study sample included 112 children aged 7-11 years in Fayette Co Kentucky. The children were divided into groups according type of education. There were 65 home-schooled children and 47 children who attend public school. Subjects recorded their dietary intake for one week. The data were analyzed using Nutrition Data Software for Research (2006). Comparisons were made for the intake of selected nutrients using two tailed independent sample t-tests.

Results: The public school …


Challenges And Opportunities To Rural Nutrition Education Programs In Kentucky's Superfund Communities, Carolyn L. Hofe Jan 2008

Challenges And Opportunities To Rural Nutrition Education Programs In Kentucky's Superfund Communities, Carolyn L. Hofe

University of Kentucky Master's Theses

The National Electric Coil Company/Cooper Industries, Inc. plant in Harlan County, Kentucky was a mining support operation primarily engaged in the cleaning and repair of mining equipment from 1951 to 1987. Trichloroethylene (TCE) and degradation byproducts, polychlorinated biphenyls (PCBs), and vinyl chloride were released into the areas surrounding the plant periodically for decades. Routine water sampling of area wells by the Kentucky Department of Environmental Protection Division of Water revealed significant levels of TCE, PCBs, and vinyl chloride. The toxicology of these chemicals implicates various systems, including cardiovascular, dermal, endocrine, and neurological.

University of Kentucky’s Superfund Basic Research Program’s (UK-SBRP) …


Parental Perceptions Of Nutritional Status Of Children With Autism, Rachel Corliss Holt Jan 2008

Parental Perceptions Of Nutritional Status Of Children With Autism, Rachel Corliss Holt

University of Kentucky Master's Theses

Literature shows that nutritional deficiency and feeding behaviors in neurodevelopmentally disabled children is secondary to an underdeveloped gastrointestinal tract. Fifty (50) parents with children with autism responded to a Nutritional Perception Assessment that they believe their children are at adequate nutritional status. They reported confidence in their nutritional knowledge, yet admitted to concern about the nutritional status of their children. The survey revealed that parents are not seeing a registered dietitian for their nutritional advice, leading to the question of soundness in nutritional information received. A marginal correlation was found between belief in adequate nutritional status of children and the …


United States Regulation Of Stem Cell Research: Recasting Government's Role And Questions To Be Resolved, Owen C. B. Hughes, Alan L. Jakimo, Michael J. Malinowski Jan 2008

United States Regulation Of Stem Cell Research: Recasting Government's Role And Questions To Be Resolved, Owen C. B. Hughes, Alan L. Jakimo, Michael J. Malinowski

Journal Articles

This article directly addresses the stem cell controversy, but also the broader history and norms regarding the roles of federal and state government in U.S. science research funding.


Exercise Training Improves Premenopausal Women's Ability To Estimate Energy Expenditure After Weight Loss, Micah D. Greer Jan 2008

Exercise Training Improves Premenopausal Women's Ability To Estimate Energy Expenditure After Weight Loss, Micah D. Greer

All ETDs from UAB

Objective: Previously we have shown that total free living energy expenditure (TEE) and greater fitness was associated with reduced long term weight gain and improved ability to estimate TEE from physical activity (PA) questionnaires. In the current study, it is hypothesized that women will overestimate questionnaire self reported energy expenditure (QEE). Furthermore, weight reduced women who exercise train will be better able to estimate TEE compared to weight reduced women who do not exercise train. Design: The participants consisted of 35 European American (EA) and 49 African American (AA) pre-menopausal women. These women were evaluated while overweight and when weight …


Geographic Differences In Accessibility To Renal Treatment Among Black And White Populations Within Network 6 Of The End Stage Renal Disease Networks, Shenee J. Reid Jan 2008

Geographic Differences In Accessibility To Renal Treatment Among Black And White Populations Within Network 6 Of The End Stage Renal Disease Networks, Shenee J. Reid

All ETDs from UAB

Objective: This study examined relationships between racial composition of zip codes and the geographic accessibility to dialysis centers of Network 6 of the End Stage Renal Disease Networks. Study Design: Geographic accessibility to renal care (hemodialysis) was defined as the average distance between the centers of zip codes where dialysis patients reside and the dialysis centers that fall within a 30 mile buffer radius. Data Sources: This research studies the distribution of 33,536 patients, 1,798 zip codes, and 546 dialysis centers in Georgia, North Carolina, and South Carolina as of December 31, 2007. Information and data concerning the dialysis centers …


Strafford County 2007 Annual Report Of The Commissioners, Treasurer, Other County Officers And The Strafford County Delegation Strafford County, New Hampshire For The Year Ending December 31, 2007., Stafford County Representatives Jan 2008

Strafford County 2007 Annual Report Of The Commissioners, Treasurer, Other County Officers And The Strafford County Delegation Strafford County, New Hampshire For The Year Ending December 31, 2007., Stafford County Representatives

Strafford County, NH Annual Reports

This is an annual report containing vital statistics for a county in the state of New Hampshire.


Food Insecurity And Maternal Depression In Rural, Low-Income Families: A Longitudinal Investigation, Catherine Huddleston-Casas, Richard Charnigo, Leigh Ann Simmons Jan 2008

Food Insecurity And Maternal Depression In Rural, Low-Income Families: A Longitudinal Investigation, Catherine Huddleston-Casas, Richard Charnigo, Leigh Ann Simmons

Nebraska Center for Research on Children, Youth, Families, and Schools: Faculty Publications

Objective: The purpose of the present study was to examine the relationship between household food insecurity and maternal depression in a rural sample to determine whether food insecurity predicted mothers’ depression over time or vice versa.

Design: The study employed a prospective design using three waves of data from ‘Rural Families Speak’, a multi-state study of low-income rural families in the USA. Food insecurity was measured using the Core Food Security Module and depression was measured using the Center for Epidemiologic Studies–Depression Scale. A structural equation model was fit to the data using the AMOS software package.

Setting: Sixteen states …


Comparison Of Amylose Determination Methods And The Development Of A Dual Wavelength Iodine Binding Technique, Thianming Zhu, David S. Jackson, Randy L. Wehling, Bhima Geera Jan 2008

Comparison Of Amylose Determination Methods And The Development Of A Dual Wavelength Iodine Binding Technique, Thianming Zhu, David S. Jackson, Randy L. Wehling, Bhima Geera

Department of Food Science and Technology: Faculty Publications

It has long been recognized that limitations exist in the analytical methodology for amylose determination. This study was conducted to evaluate various amylose determination methods. Purified amylose and amylopectin fractions were obtained from com, rice, wheat, and potato and then mixed in proportion to make 10, 20, 30, 50, and 80% amylose content starch samples for each source. These samples, considered amylose standards, were analyzed using differential scanning calorimetry (DSC), high-performance size-exclusion chromatography (HPSEC), and iodine binding procedures to generate standard curves for each of the methods. A single DSC standard equation for cereal starches was developed. The standard curve …


Weight Changes In Wild Wolves, Canis Lupus, From Ages 2 To 24 Months, L. David Mech Jan 2008

Weight Changes In Wild Wolves, Canis Lupus, From Ages 2 To 24 Months, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Weights of 118 female and 141 male Minnesota Wolves (Canis lupus) aged 2-24 months increased almost linearly from about 8 kg for females and 10 kg for males at 3 months to 30 kg for females and 32 kg for males at 10-12 months and then tended to increase much more slowly in an overall curvilinear trend. Considerable variation was apparent for both sexes during their first year.


The Wetlands Reserve Program Supports Migrating Waterfowl In Nebraska’S Rainwater Basin Region Jan 2008

The Wetlands Reserve Program Supports Migrating Waterfowl In Nebraska’S Rainwater Basin Region

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The Rainwater Basin region (RWB) consists of a 6,150 mi2 area of loess plains in south-central Nebraska (figure 1). The area is characterized by rolling plains formed by deep deposits of windblown silt with a high density of claypan playa wetlands. More than 200,000 acres of wetlands once existed in this region. As a result of agricultural and other development, only 17 percent of the original playa wetland area remains, most with hydrologic alterations. Siltation and colonization by invasive plant species (e.g., reed canary grass, narrow-leaved cattail, river bulrush) continue to threaten remaining wetland habitats in the RWB.

Despite historic …


Varying Efficiency Of Long-Term Replication Of Papillomaviruses In Saccharomyces Cerevisiae, Adam J. Rogers, Malte Loggen, Karen Lee, Peter C. Angeletti Jan 2008

Varying Efficiency Of Long-Term Replication Of Papillomaviruses In Saccharomyces Cerevisiae, Adam J. Rogers, Malte Loggen, Karen Lee, Peter C. Angeletti

Nebraska Center for Virology: Faculty Publications

Human papillomaviruses (HPVs) replicate in mitotically active basal keratinocytes. Two virally encoded proteins, E1, a helicase, and E2, a transcription factor, are important players in replication and maintenance of HPV episomes. We previously showed that HPV16 could replicate stably in Saccharomyces cerevisiae [Angeletti, P.C., Kim, K., Fernandes, F.J., and Lambert, P.F. (2002)] and we identified cis-elements that mediate replication and maintenance [J. Virol. 76(7), 3350-3358.; Kim, K., Angeletti, P.C., Hassebroek, E.C., and Lambert, P.F. (2005)]. Here, we demonstrate that although multiple HPV genomes replicate stably in yeast, they do so with differing long-term efficiency; HPV6-Ura3 is replicated at the …


Diets Of Lesser Scaup During Spring Migration Throughout The Upper-Midwest Are Consistent With The Spring Condition Hypothesis, Michael J. Anteau, Alan Afton Jan 2008

Diets Of Lesser Scaup During Spring Migration Throughout The Upper-Midwest Are Consistent With The Spring Condition Hypothesis, Michael J. Anteau, Alan Afton

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

The spring condition hypothesis (SCH) states that the current decline of the North American scaup population (Lesser [Aythya affinis] and Greater Scaup [A. marila] combined) is due to a decline in quality or availability of scaup foods on wintering, spring migration, or breeding areas that has caused a reduction in female body condition and subsequent reproductive success. Our previous research indicated that forage quality in diets of Lesser Scaup (hereafter scaup) at two sites in Northwestern Minnesota was lower in springs 2000-2001 than that reported for springs 1986-1988, consistent with the SCH. Accordingly, we further tested …


Evaluation Of Aerial Cluster Sampling Of Double-Crested Cormorants On Aquaculture Ponds In Mississippi, Brian S. Dorr, Loren W. Burger Jr., Scott C. Barras Jan 2008

Evaluation Of Aerial Cluster Sampling Of Double-Crested Cormorants On Aquaculture Ponds In Mississippi, Brian S. Dorr, Loren W. Burger Jr., Scott C. Barras

United States Department of Agriculture Wildlife Services: Staff Publications

Concern over increasing numbers of double-crested cormorants (Phalacrocorax auritus) and their impacts on channel catfish (Ictalurus punctatus) aquaculture has resulted in increased need for quantitative information to develop and evaluate depredation management efforts. We evaluated aerial surveys in a stratified cluster sampling (SCS) design to estimate and monitor abundance of cormorants on catfish aquaculture ponds in the Yazoo River Basin of Mississippi, USA (hereafter Yazoo Basin). Twice monthly abundance estimates and coefficient of variation during winter averaged 8,128 (n = 29, SE = 1,233) and 33% (n = 29, SE = 0.02), respectively. Counts of cormorants …


Importance And Effect Of Nitrogen On Crop Quality And Health, Jürg Blumenthal, David D. Baltensperger, Kenneth G. Cassman, Stephen Mason, Alexander Pavlista Jan 2008

Importance And Effect Of Nitrogen On Crop Quality And Health, Jürg Blumenthal, David D. Baltensperger, Kenneth G. Cassman, Stephen Mason, Alexander Pavlista

Department of Agronomy and Horticulture: Faculty Publications

Nitrogen (N) is often the most limiting factor in crop production. Hence, application of fertilizer nitrogen results in higher biomass yields and protein yield and concentration in plant tissue is commonly increased. Nitrogen often affects amino acid composition of protein and in turn its nutritional quality. In cereals, abundant supply of nitrogen decreases the relative proportion of lysine and threonine, thus, reducing the biological value of the protein. Increasing nitrogen supply generally improves kernel integrity and strength, resulting in better milling properties of the grain. In oil seed crops, protein levels are increased upon nitrogen fertilization, whereas oil concentration is …


A Case Study To Teach The Diagnostic Process: Determining The Cause Of Chlorosis In A Crop Of Cut Dicentra, Marci Spaw, Kimberley A. Williams, Laurie Hodges, Ellen T. Paparozzi, Ingrid L. Mallberg Jan 2008

A Case Study To Teach The Diagnostic Process: Determining The Cause Of Chlorosis In A Crop Of Cut Dicentra, Marci Spaw, Kimberley A. Williams, Laurie Hodges, Ellen T. Paparozzi, Ingrid L. Mallberg

Department of Agronomy and Horticulture: Faculty Publications

This universally accessible, Web-based decision case presents the challenge of determining the cause of foliar chlorosis in a crop of dicentra (Dicentra spectabilis) being forced as a cut flower for Valentine's Day sales. The case study serves as a tool to promote the development of diagnostic skills for production dilemmas, including nutritional disorders, disease problems, and evaluation of the appropriateness of cultural practices, Cut dicentra is a minor crop and standard production practices are not well established. Solving this case requires that students research production protocol, as well as nutritional and pest problems, and determine whether they have …


Characterization Of The Folate Salvage Enzyme P-Aminobenzoylglutamate Hydrolase In Plants, Gale G. Bozzo, Gilles J. C. Basset, Valeria Naponelli, Alexandre Noiriel, Jesse F. Gregory Iii, Andrew D. Hanson Jan 2008

Characterization Of The Folate Salvage Enzyme P-Aminobenzoylglutamate Hydrolase In Plants, Gale G. Bozzo, Gilles J. C. Basset, Valeria Naponelli, Alexandre Noiriel, Jesse F. Gregory Iii, Andrew D. Hanson

Department of Agronomy and Horticulture: Faculty Publications

Folates break down in vivo to give pterin and p-aminobenzoylglutamate (pABAGlu) fragments, the latter usually having a polyglutamyl tail. Pilot studies have shown that plants can hydrolyze pABAGlu and its polyglutamates to p-aminobenzoate, a folate biosynthesis precursor. The enzymatic basis of this hydrolysis was further investigated. pABAGlu hydrolase activity was found in all species and organs tested; activity levels implied that the proteins responsible are very rare. The activity was located in cytosol/vacuole and mitochondrial fractions of pea (Pisum sativum L.) leaves, and column chromatography of the activity from Arabidopsis tissues indicated at …


Whole-Plant Mineral Partitioning Throughout The Life Cycle In Arabidopsis Thaliana Ecotypes Columbia, Landsberg Erecta, Cape Verde Islands, And The Mutant Line Ysl1ysl3, Brian M. Waters, Michael A. Grusak Jan 2008

Whole-Plant Mineral Partitioning Throughout The Life Cycle In Arabidopsis Thaliana Ecotypes Columbia, Landsberg Erecta, Cape Verde Islands, And The Mutant Line Ysl1ysl3, Brian M. Waters, Michael A. Grusak

Department of Agronomy and Horticulture: Faculty Publications

Minimal information exists on whole-plant dynamics of mineral flow through Arabidopsis thaliana or on the source tissues responsible for mineral export to developing seeds. Understanding these phenomena in a model plant could help in the development of nutritionally enhanced crop cultivars. A whole-plant partitioning study, using sequential harvests, was conducted to characterize growth and mineral concentrations and contents of rosettes, cauline leaves, stems, immature fruit, mature fruit hulls, and seeds of three WT lines (Col-0, Ler, and Cvi) and one mutant line (Col-0::ysl1ysl3). Shoot mineral content increased throughout the life cycle for all minerals, although tissue-specific …


Comparison Of Lipid Extraction Methods Of Food-Grade Sorghum (Sorghum Bicolor) Using Hexane, K. L. Christiansen, Curtis L. Weller, V. L. Schlegel, I. M. Dweikat Jan 2008

Comparison Of Lipid Extraction Methods Of Food-Grade Sorghum (Sorghum Bicolor) Using Hexane, K. L. Christiansen, Curtis L. Weller, V. L. Schlegel, I. M. Dweikat

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Much has been reported concerning sorghum phytochemicals, such as policosanols, but little literature exists on how various extraction methods might be used to optimize the recovery of such phytochemicals. The purpose of this study was to compare three extraction methods for recovery of different sorghum lipids. Lipids were extracted from whole kernel and ground Macia, a food-grade sorghum hybrid, with hexane by a Soxtec method, a refluxing method (RB), and a bench-scale recirculated solvent (RC) method. Comparisons of total lipid yield and lipid class yields extracted from the grain by each method were made. Total lipid yield for the three …


Reduced Reliance On The Trace Element Selenium During Evolution Of Mammals, Alexey Lobanov, Dolph Hatfield, Vadim N. Gladyshev Jan 2008

Reduced Reliance On The Trace Element Selenium During Evolution Of Mammals, Alexey Lobanov, Dolph Hatfield, Vadim N. Gladyshev

Vadim Gladyshev Publications

Background: Selenium (Se) is an essential trace element that occurs in proteins in the form of selenocysteine (Sec). It is transported throughout the body in the form of Sec residues in Selenoprotein P (SelP), a plasma protein of unclear origin recently proposed as an experimental marker of dietary Se status.
Results: Here, we report that the amino-terminal domain of SelP is distantly related to ancestral bacterial thiol oxidoreductases of the thioredoxin superfamily, and that its carboxy-terminal Se transport domain may have originated in early metazoan evolution by de novo accumulation of Sec residues. Reconstruction of evolutionary changes in the Se …


A Functional Link Between Housekeeping Selenoproteins And Phase Ii Enzymes, Aniruddha Sengupta, Bradley A. Carlson, James A. Weaver, Sergey V. Novoselov, Dmitri E. Fomenko, Vadim N. Gladyshev, Dolph Hatfield Jan 2008

A Functional Link Between Housekeeping Selenoproteins And Phase Ii Enzymes, Aniruddha Sengupta, Bradley A. Carlson, James A. Weaver, Sergey V. Novoselov, Dmitri E. Fomenko, Vadim N. Gladyshev, Dolph Hatfield

Vadim Gladyshev Publications

Sec (selenocysteine) is biosynthesized on its tRNA and incorporated into selenium-containing proteins (selenoproteins) as the 21st amino acid residue. Selenoprotein synthesis is dependent on Sec tRNA and the expression of this class of proteins can be modulated by altering Sec tRNA expression. The gene encoding Sec tRNA (Trsp) is a single-copy gene and its targeted removal in liver demonstrated that selenoproteins are essential for proper function wherein their absence leads to necrosis and hepatocellular degeneration. In the present study, we found that the complete loss of selenoproteins in liver was compensated for by an enhanced expression of several phase II …


Identification Of A Consensus Dna-Binding Site For The Arabidopsis Thaliana Sbp Domain Transcription Factor, Atspl14, And Binding Kinetics By Surface Plasmon Resonance, Xinwen Liang, Tara J. Nazarenus, Julie M. Stone Jan 2008

Identification Of A Consensus Dna-Binding Site For The Arabidopsis Thaliana Sbp Domain Transcription Factor, Atspl14, And Binding Kinetics By Surface Plasmon Resonance, Xinwen Liang, Tara J. Nazarenus, Julie M. Stone

Department of Biochemistry: Faculty Publications

Proteins with a conserved Cys- and His-rich SQUAMOSA promoter binding protein (SBP) domain are transcription factors restricted to photosynthetic organisms that possess a novel two Zn-finger structure DNA-binding domain. Despite the fact that altered expression of some SBP-encoding genes has profound effects on organism growth and development, little is known about SBP domain protein target genes. Misexpression of the Arabidopsis thaliana AtSPL14 SBP domain gene confers resistance to programmed cell death and modifies plant architecture. A consensus DNA-binding motif for AtSPL14 was identified by systematic evolution of ligands by exponential enrichment (SELEX) or random binding site selection (RBSS). DNA recognized …