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Regulation Of The Human Parainfluenza Virus (Hpiv3) Fusion Protein, Amanda Ruth Chapman

Theses and Dissertations (ETD)

Paramyxoviruses include a number of important human pathogens, including measles virus, mumps virus, and the human parainfluenza viruses (hPIV) 1-4, as well as several animal pathogens, such as Sendai virus, Nipah virus and Hendra virus. The creation of effective drugs and vaccines against this family of viruses would play an important role in decreasing the prevalence of these viruses and contributing to the health of both humans and animals worldwide. The purpose of this work was to determine how the fusion (F) protein is regulated with a focus on the heptad repeat B (HRB) region of the F protein located …

C-Reactive Protein Polymorphism And Serum Levels As An Independent Risk Factor In Sickle Cell Disease, Elizabeth A. Chismark

Theses and Dissertations (ETD)

This study explored the relationship of a dinucleotide repeat polymorphism in the intron of the CRP gene and serum CRP levels as independent risk factors for end-organ dysfunction (mild vs. severe) in adults with sickle cell disease. The pathogenesis of secondary complications of sickle cell disease is complex and poorly understood. Predicting the severity of these complications could assist in therapeutic decision-making.

The study measured serum CRP levels and the number of CA intron repeats located on the CRP gene in 29 adults (31.74 ± 11.54 years) with sickle cell disease The hemoglobin genotypes were distributed as Hgb SS 48.6% …

Role Of The Lpa2 Receptor In Protecting Against Apoptosis, Shuyu E

Theses and Dissertations (ETD)

Lysophosphatidic acid (LPA) is a naturally occurring lipid mediator. It exists abundantly in biological fluids such as serum, saliva, follicular fluid, seminal fluid and malignant effusions and induces a vast array of biological responses affecting cell growth, survival, differentiation, migration and morphology. We recently identified lysophosphatidic acid (LPA) as a potent antiapoptotic agent for the intestinal epithelium. Based on computational modeling octadecenyl thiophosphate (OTP) was synthesized: a novel rationally designed, metabolically stabilized LPA mimic. OTP was more efficacious than LPA in reducing g-irradiation-, camptothecin-, or TNF-a/cycloheximide-induced apoptosis and caspase 3, 8 and 9 activity in the IEC-6 cell line. The …

The Role Of Fgf Signaling In Retinal Development, Abbie A. Hartge

Theses and Dissertations (ETD)

The development of the retina is a precise balance between intrinsic competence and extrinsic factors. This interplay is known to regulate the generation of cell types in the developing retina and similar mechanisms have been found in other regions of the CNS. In the developing retina, FGFs are a large family of secreted polypeptide growth factors. Fgf15 is the major Fgf expressed during retinal development in mice. Fgf15 is an example of an FGF that has been shown to control proliferation, cell fate specification, differentiation and migration during development. In this thesis I used analysis of specific genes throughout retinal …

Predictors Of Depressive Symptoms And Obesity In African American Women Transitioning From Welfare To Work, Mayola Rowser

Theses and Dissertations (ETD)

Depression and obesity are significant public health problems that adversely affect the health and quality of life of women in the United States, particularly female African American welfare recipients transitioning to work. African American women are twice as likely to experience depressive symptoms as women of other races. Fifty percent of African American women are obese. The prevalence of depression and depressive symptoms and obesity is higher among African American women who receive welfare benefits. These health conditions jeopardize their health status, ability to work, and chances to become self-sufficient. To ensure the successful transition of recipients to work, health …

The Tetraspanin Cd9 Localizes To Platelet-Platelet Contacts And Regulates Thrombus Stability, Sarah Kathleen Hill

Theses and Dissertations (ETD)

CD9, a member of the tetraspanin family, is highly expressed on platelets (50,000-80,000 copies per platelet). Tetraspanins have been implicated in modulation of integrin function, and it is hypothesized that CD9 will modulate GPIIb-IIIa, the major platelet integrin.

The association between CD9 and GPIIb-IIIa was analyzed using immunoprecipitations and confocal microscopy. These two proteins were found to associate with each other, particularly at areas of platelet-platelet contact and at the periphery. Confocal analysis revealed CD9 localization was most intense at platelet-platelet contact, as well as in platelet filopodia and lamellipodia, but there is a lack of CD9 at areas of …

Synthesis And Evaluation Of Tetramic Acids As Antimicrobial Agents, Jason B. Wilson

Theses and Dissertations (ETD)

As bacterial infectious diseases are a major cause of morbity and mortality throughout the world, and many causative organisms are resistant to currently available antibiotics, the motivation for the development of new drugs is readily apparent. A number of natural products exhibiting antimicrobial activity possess a tetramic acid (2,4-pyrrolidinedione) functional group. As their antibacterial mechanism of action is different from that of many of the currently available antibiotics, these compounds have potential to serve as a basis for a pharmacophore in synthetic compounds. However, toxicity to eukaryotic cells is frequently a problem with currently known tetramic acids. The purpose of …

Factors Affecting Long-Term Outcomes Following Intensive In-Home Services, Sarah Hurley

Theses and Dissertations (ETD)

Intensive in-home services (IIHS) work with families toward building skills and resources to better manage their children with behavioral difficulties. Factors that impact long-term outcomes following IIHS are not well understood. This study examined the relative importance of youth demographic and clinical characteristics, family history variables, characteristics of program participation, and organizational-level factors in explaining placement stability, educational progress, and contact with legal authorities one year following discharge from IIHS.

The sample included all youth who received IIHS, were discharged between January 1, 2001 and December 31, 2006, and completed a follow up at one-year post-discharge (n = 2,649) from …

Studies Of Entry, Reverse Transcription, And Regulation Of Splicing In Retroviruses, Timothy A. Sullivan

Theses and Dissertations (ETD)

The study of retroviruses and their lifecycle has contributed immensely to our knowledge of the world of biology and medicine. The central dogma of the basic flow of genetic information was shattered when the discovery that retroviruses copy their RNA genome into DNA was made. The same enzyme that performs this step, reverse transcriptase (RT), also revolutionized molecular biology when it was used as a tool to generate full length cDNA clones of expressed genes. The impact of retroviruses on the medical field has been extremely exciting as the ideas of using retroviral vectors to deliver genes providing long term …

Use Of Compliant Interbody Force Sensing Grafts To Compare Load Sharing Properties Of Unidirectional And Bidirectional Multilevel Dynamic Translational Anterior Cervical Plates, Jason Roscoe Roberson

Theses and Dissertations (ETD)

INTRODUCTION: Pseudoarthrosis is a relatively rare complication following anterior cervical arthrodesis, and is felt to be related to stress shielding. To address this, dynamic anterior cervical plates have emerged to maintain load sharing as an arthodesis matures. Dynamic plates can translate bidirectionally (allow translation in compressive and tensile loads), or unidirectionally (allow translation in compression, but maintain its position under tensile loads). The objective of this study was to compare the graft load mechanics between three plated conditions during settling using compliant interbody load cells: A static (fixed, non-moving) plate, a unidirectional multi-level translational plate design, and a bidirectional two-level …

Structure- And Ligand-Based Design Of Novel Antimicrobial Agents, Kirk Edward Hevener

Theses and Dissertations (ETD)

The use of computer based techniques in the design of novel therapeutic agents is a rapidly emerging field. Although the drug-design techniques utilized by Computational Medicinal Chemists vary greatly, they can roughly be classified into structure-based and ligand-based approaches. Structure-based methods utilize a solved structure of the design target, protein or DNA, usually obtained by X-ray or NMR methods to design or improve compounds with activity against the target. Ligand-based methods use active compounds with known affinity for a target that may yet be unresolved. These methods include Pharmacophore-based searching for novel active compounds or Quantitative Structure-Activity Relationship (QSAR) studies. …

A Kinematics-Based Testing Protocol To Study The Mechanics Of The Human Lumbar Spine, Nephi A. Zufelt

Theses and Dissertations (ETD)

The objective of this work was to develop a new kinematics-based testing protocol to quantify the axial and shear force components and rotational moment properties of the human cadaveric lumbar motion segment unit (MSU) in response to specific kinematic inputs. Modern, non-fusion spinal devices claim to treat degenerative disc disease better than traditional fusion surgery. Though there have been many biomechanical studies completed on these devices, there is still a debate over their efficacy. Conventional testing methods provide insight into the rotational properties of the MSU but lack the sensitivity or capacity to quantify lumbar MSU’s mechanical properties including shear …

Torsina And The Pathophysiology Of Dyt1 Dystonia, Yu Zhao

Theses and Dissertations (ETD)

The goal of my dissertation work was to examine the systems biology of torsinA, a DYT1 dystonia-associated protein, by using rodent model systems. TorsinA is a putative ATPase associated with a variety of cellular activities (AAA+). Deletion of glutamic acid residue 302/303 in TOR1A is causally associated with many cases of early-onset primary dystonia.

In our work, transient forebrain ischemia and sciatic nerve transection were used as central and peripheral neural perturbations, respectively, to gain insight into the in vivo role(s) of torsinA. Moreover, transgenic mouse models that overexpress either human mutant torsinA (hMT) or wild-type torsinA (hWT) were used …

Interaction Of Bacteriophage Mu Middle Transcription Activator Protein Mor With Promoter Dna, Kartik Iyer

Theses and Dissertations (ETD)

Gene expression during lytic development of bacteriophage Mu is regulated by a

transcriptional cascade in three phases: early, middle and late. Transcription from the middle promoter Pm requires the 129-amino acid transcriptional activator Mor, a product of early transcription, and the Escherichia coli RNA polymerase. The Pm promoter has a recognizable -10 hexamer but lacks a -35 hexamer. Mor binds as a dimer to an imperfect dyad-symmetrical element containing two 6-bp inverted repeats and centered at -43.5 in Pm. The goals of this study were:

1. To test the prediction from the crystal structure of Mor that residues Y70 and …

Controlled Release Of Insulin And Modified Insulin From A Novel Injectable Biodegradable Gel, Om Anand

Theses and Dissertations (ETD)

The objective of the study was to develop a controlled release dosage form of
insulin, which can provide basal concentrations of insulin in diabetic rats for 1 to 2 weeks after a single subcutaneous injection.
A biodegradable injectable drug delivery gel was prepared by dissolving a
biodegradable polymer, polylactic-co-glycolic acid (PLGA), in biocompatible
plasticizer(s), triethyl citrate (TEC) and/or acetyl triethyl citrate (ATEC). Insulin was
then loaded into the blank gel to form an insulin suspension in the polymer solution.
After the insulin-loaded gel was injected subcutaneously, the plasticizer(s) dissolved in the aqueous media and were gradually taken away from the …

Reconciling Soccer Authorities And European Union Institutions: Who Is Best Placed To Administer Governance Within The European Soccer Market?, Duncan Mchardy

Seton Hall Journal of Sports and Entertainment Law

No abstract provided.

A Family Affair: Extending The Right Of Publicity To Protect Celebrity Children, Sara Kimball

Seton Hall Journal of Sports and Entertainment Law

No abstract provided.

Warren Spahn's Legal Legacy: The Right To Be Free From False Praise, Ray Yasser

Seton Hall Journal of Sports and Entertainment Law

No abstract provided.

Concussions In The National Football League: Jani V. Bert Bell/Pete Rozelle Nfl Player Ret. Plan And A Legal Analysis Of The Nfl's 2007 Concussion Management Guidelines, Jennifer Ann Heiner

Seton Hall Journal of Sports and Entertainment Law

No abstract provided.

Water Status Of Breastfed Infants During Weaning In Yazd, Iran, Mehrdad Shakiba, Talie Montehaee, Zahra Nafei, Mohamad H. Falahzade

Saudi Medical Journal

OBJECTIVE: To investigate the state of hydration in infants during the weaning period in dry and hot climates in the center of Iran. METHODS: Using a refractometer, 162 urine specific gravity (USG) was measured from 400 infants, between 4 to 6 months old. They were chosen among infants who visited the primary clinics in the city of Yazd, Iran for routine vaccination during the summer and winter of 2005. A questionnaire was filled out on air conditioning system and diet from mothers. A USG >1.020 was accepted as dehydrated, and a USG <1.010 as well hydrated. RESULTS: Thirty-one percent of the infants were dehydrated, and 34% were well hydrated, infants were more dehydrated in summer (p<0.05). Twenty-seven percent of them started solid food without water supplementation, and dehydration was significant in most of them (p<0.05). We could not determine which type of air conditioning devices now used at home affect water status, but there was a trend toward dehydration in those using both fan and cooler (p=0.096). CONCLUSION: In the desert area in the center of Iran, during weaning, approximately one third of the infants are exposed to the danger of dehydration. Our primary health care provider should consider the priority of water during weaning when water supplementation is safe.

Community-Based Screening For Pre-Hypertension Among Military Active Duty Personnel, Saeed M. Al-Asmary, Abdulsalam A. Al-Shehri, Fayssal M. Farahat, Moataz M. Abdel-Fattah, Muraya M. Al-Shahrani, Fahd K. Al-Omari, Fawaz S. Al-Otaibi, Dhifallah M. Al-Malki

Saudi Medical Journal

OBJECTIVE: To determine the prevalence of both pre-hypertension and hypertension, and risk factors associated with the newly diagnosed Saudi military active duty personnel. METHODS: A community-based cross-sectional screening of 1238 Saudi military active duty service personnel was conducted during the period from September to December 2007 at the military units of Taif region, western Saudi Arabia. Screening tools included self-administrated questionnaire, general physical examination, anthropometric measurements, and assessment of blood pressure. RESULTS: All participants were Saudi males. Their age ranged from ±7.02. By applying the Joint National Committee on prevention, detection, evaluation, and treatment of high hypertension criteria, 214 (17.3%) …

Prevalence Of Dysglycemia And Other Cardiovascular Risk Factors Among The Rural Population Of Oman, Mohammed N. Barakat, Randa M. Youssef

Saudi Medical Journal

No abstract provided.

The Failure Of Punitive Damages In Employment Discrimination Cases: A Call For Change, Joseph A. Seiner

William & Mary Law Review

Punitive damages were described by one early court as "an unsightly and an unhealthy excrescence." Although views toward punitive relief have changed over the years, the debate over the availability of exemplary damages in the judicial system has remained controversial. No place is that controversy more aptly demonstrated than in employment discrimination law, where punitive damages first became available in an amendment to Title VII of the Civil Rights Act of 1964 after a bitter congressional debate. Almost a decade ago, in Kolstad v. American Dental Association, the Supreme Court provided guidance on how punitive damages should be applied in …

Self-Consciousness Moderates The Relationship Between Perceived Norms And Drinking In College Students, Joseph W. Labrie, Justin F. Hummer, Clayton Neighbors

Heads Up!

The current research examines whether self-consciousness subscales have prognostic value in the relationship between perceived norms and drinking and if that differs among college men and women. Results indicate that self-consciousness moderates gender differences in the relationship between perceived social norms and drinking. A strong positive relationship was found between perceived norms (descriptive and injunctive) and drinking for men relative to women and this was more pronounced among individuals who were lower in public self-consciousness. Similarly, the relationship between perceived injunctive norms and drinking was significantly stronger among men than women and this was more pronounced among individuals who were …

Changes In Drinking Patterns Across The Transition To College Among First-Year College Males, Joseph W. Labrie, Toby Lamb, Eric Pedersen

Heads Up!

Few studies examine changes in drinking behavior during the transition from high school to college. Using a sample of 239 first-year males, we hypothesized that participants would increase drinking from pre-college to the first month of college. Results reveal a general trend toward increased drinking upon entering college. Caucasians increased drinking more than non-Caucasians. Social expectancies of alcohol moderated increases in drinking behavior. These findings indicate that differential changes in drinking behavior occur among incoming college males. Interventions with college students need to address both preventing heavy consumption and alcohol-related problems in pre-college light drinkers and in reducing these behaviors …

Estrogen And Hippocampal Plasticity In Rodent Models, Michael R. Foy, Michel Baudry, Roberta Diaz Brinton, Richard F. Thompson

Psychological Science Faculty Works

Accumulating evidence indicates that ovarian hormones regulate a wide variety of non-reproductive functions in the central nervous system by interacting with several molecular and cellular processes. A growing animal literature using both adult and aged rodent models indicates that 17β-estradiol, the most potent of the biologically relevant estrogens, facilitates some forms of learning and memory, in particular those that involve hippocampal-dependent tasks. A recently developed triple-transgenic mouse (3xTg-AD) has been widely used as an animal model of Alzheimer's disease, as this mouse exhibits an age-related and progressive neuropathological phenotype that includes both plaque and tangle pathology mainly restricted to hippocampus, …

Theorizing Destruction: Reflections On The State Of Comparative Genocide Theory, Maureen S. Hiebert

Genocide Studies and Prevention: An International Journal

This article reviews the current state of comparative genocide theorizing, focusing on theories that attempt to account for the causes of genocide and the processes of genocidal killing. The literature is divided into three broad categories, based on the relative weight given to (a) individual or group agency, (b) structural factors, or (c) processes of identity construction in accounting for the origins and unfolding of genocidal destruction. The discussion of agency- oriented approaches focuses on theories that suggest that genocide is driven, in terms of decision making and perpetration, by elite decision makers, front-line perpetrators, and societal behavior. The literature …

Perpetual Conflict Or Compromise? The Cost Of Domestic Legitimacy In The Realm Of Women's Human Rights: A Case Study On The Right To An Abortion, Kim Andrea Kelly

Honors Scholar Theses

With its turbulent and volatile legal evolution, the right to an abortion in the United States still remains a highly contested issue and has developed into one of the most divisive topics within modern legal discourse. By deconstructing the political underpinnings and legal rationale of the right to an abortion through a systematic case law analysis, I will demonstrate that this right has been incrementally destabilized. This instability embedded in abortion jurisprudence has been primarily produced by a combination of textual ambiguity in the case law and judicial ambivalence regarding this complex area of law. In addition, I argue that …

Communicable Disease And International Security: The Case Of Africa & Hiv/Aids, Amy L Rue

Archived Theses and Dissertations

In this thesis, I will look at how communicable disease fits into the human security discourse particularly with the disease being views as the 'potential threat' and the 'referent object' within the international sphere. The case of Africa will be used to illustrate the impact of HIV/AIDS on international security at country, regional and international levels. Ultimately, I will argue that communicable disease, as a human security issue, is a clear and important international security issue due to its status as a continual "potential threat" within international relations particularly in Africa. The elements that I will use to establish my …

Cognitive Development And The Understanding Of Informed Consent, Lindsey Elizabeth Pearse

Masters Theses and Doctoral Dissertations

The informed consent process is an important criterion for all research studies. However, researchers rarely ask or even consider how many participants truly understand the informed consent information and their research rights. Currently, researchers base a participant’s ability to understand informed consent information and research rights on age. In the present study it was hypothesized that cognitive development is a predictor of ethical knowledge. Accordingly, it was argue d that a participant’s ability to understand informed consent and research rights should not be based on a participant’s age but instead on the participant’s cognitive development. Students at the University of …