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Protocols For Preconditioning Of Patellar Tendon For Anterior Cruciate Ligament Reconstruction, Richard Lee Crawford

Theses and Dissertations

The ACL is one of the major ligaments in the knee connecting the femur to the tibia which provides stability by resisting shear in sagittal plane. ACL tears occur in 1 out of 3000, and due to inability to heal, reconstructive surgeries are performed at a rate of 200,000/year. Final graft fixation tension during surgery has been shown to wane due to stress-relaxation which has been correlated with negative clinical outcomes. Therefore, preconditioning, which currently is an isometric load (88N), is performed to remove stress-relaxation after the final tension has been applied in vivo. Three preconditioning protocols, creep, stress relaxation, …

Relationship Between Nurse Educators' Cultural Competence And Ethnic Minority Nursing Students' Recruitment And Graduation., Pearl Ngozika Ume-Nwagbo

Electronic Theses and Dissertations

The purpose of this exploratory study was to measure the cultural competence of nurse educators in accredited baccalaureate (BSN) nursing programs in Tennessee (TN) and investigate the relationship, if any, between nurse educators' cultural competence and the percentage of minority nursing students recruited into and graduated from these schools in the previous 5 years.

With the rapid rise of the minority population in the United States, more minority healthcare providers, including nurses, are needed to provide culturally congruent care in underserved communities. Literature has implied that nurse educators' lack of cultural competence and sensitivity regarding minority nursing students' educational needs …

Bovine Coronary Region Keratinocyte Colony Formation Is Supported By Epidermal-Dermal Interactions, J.A. Mills, D.S. Zarlenga, R.M. Dyers

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Delineating the factors that orchestrate keratinocyte growth and differentiation in the claw is pivotal to understanding the quality of hoof horn production in health and disease. The specific objectives of this in- vestigation were to establish an in vitro culture system for bovine coronary region keratinocytes and dermal fibroblasts, determine the colony-forming capacity of epidermal keratinocytes in the coronary region, and characterize transcriptional changes in specific cy- tokine, growth factor, and receptor genes during colony formation in coculture. Fibroblasts and keratinocytes from the coronary region of the lateral, hind limb claw were collected, and 5.0 × 103 and 7.5 × …

Availability And Seasonal Use Of Diurnal Roosts By Rafinesque's Big-Eared Bat And Southeastern Myotis In Bottomland Hardwoods Of Mississippi, Candice Leeann Stevenson

Theses and Dissertations

Rafinesque’s big-eared bat (Corynorhinus rafinesquii) and southeastern myotis (Myotis austroriparius) are listed as species of concern in Mississippi. They use bottomland hardwood forests for roosting habitat; however, much of these forests in Mississippi have been lost or degraded. I seek to characterize availability and evaluate use of diurnal tree roosts for these presumably rare bats. Approximately 1,250 ha of bottomland hardwood forest on Noxubee National Wildlife Refuge were surveyed. I measured characteristics of 622 cavity trees. Analyses revealed that these bats most often used cavities of large diameter trees (≥70 cm DBH). Rafinesque’s big-eared bat and southeastern myotis roosted commonly …

Social Capital Determinants Of Environmentalism In Spatial Context, Kim Marie Steil

Theses and Dissertations

Sociological studies of society-environment interactions are based on a premise that population well-being is dependent on the environment. Here, I argue that not only are people innately connected to the environment, but the environment also bonds people to one another. That is, people have a mutual obligation to one another to care for the environment. An often ignored factor is that individual participation toward protecting and improving the quality of the environment rests upon the local social and spatial context in which the individual is situated. The ability of individuals in a local environment to come together to identify issues, …

Characterization Of The Genes Involved In Biosynthesis And Transport Of Schizokinen, A Siderophore Produced By Rhizobium Leguminosarum Iari 917., David Jack Hammond

Electronic Theses and Dissertations

Iron is the 4th most abundant metal on the earth's crust and is required by most organisms as a cofactor for many enzymes; however, at physiological pH and aerobic conditions iron forms insoluble ferric oxyhydroxide polymers. Siderophores are low molecular weight compounds that scavenge ferric ions, bind with high affinity, and transport it into the cell via multicomponent transport systems. Rhizobia are soil dwelling organisms that form symbiotic relationships with host plants and fix atmospheric nitrogen, while the bacteria receive nutrients. R. leguminosarum IARI 917 produces a siderophore characterized as 'schizokinen'. In the present study, we have characterized the binding …

Urinary Excretion Of (1-3)-Beta-D-Glucans., Debra K. Head

Electronic Theses and Dissertations

(1→3)-β-D-Glucans are carbohydrate polymers that are present in the cell wall of various fungi and bacteria; they are pathogen associated molecular patterns that circulate during infection and modulate immunity. Our laboratory has previously established the pharmacokinetics of intravenously and orally administered glucans; the present studies investigated the renal excretion of (1→3)-β-D-glucans following intravenous and oral administration. Three fluorescently-labeled glucans were administered to adult male rats in the presence or absence of toxic challenge. Urine specimens were collected and analyzed by fluorescence spectroscopy, size-exclusion chromatography and GPC/MALLS. 71 ± 3% of fluorescence remained in the >5K MWCO fraction; this fraction showed …

Iron Acquisition In Rhodococcus Erythropolis Strain Igts8: Characterization Of A Mutant Strain That Over Produces Siderophore., Melanie Anne Pratt

Electronic Theses and Dissertations

Iron is an essential nutrient for most bacteria because enzymes like nitrate reductase and cytochromes use it as a cofactor. However, in most aerobic, neutral pH environments, iron is essentially insoluble and not easily available for bacteria to use. Many bacteria respond to this problem by releasing small organic compounds called siderophores that bind and effectively solubilize iron so that it can be transported into the cell for growth. The focus of this study was to learn more about the iron acquisition and especially the transport of iron by the soil bacterium Rhodococcus erythropolis. To fulfill this aim, mutant …

Herpes Simplex Virus Glycoprotein D/Host Cell Surface Interaction Stimulates Chlamydia Trachomatis Persistence Via A Novel Pathway., Jennifer Vanover

Electronic Theses and Dissertations

When presented with certain unfavorable environmental conditions, C. trachomatis reticulate bodies (RBs) enter into a viable, yet noncultivable state called persistence. Two hallmarks of persistent chlamydiae are swollen, aberrantly shaped RBs, as viewed by transmission electron microscopy and a decrease in infectious progeny. Several models of chlamydial persistence have been described, including interferon-γ (IFN-γ), IFN-α, IFN-β, and tumor necrosis factor-α-exposure and nutrient deprivation. Previously, we established an in vitro co-infection model of two of the most common sexually transmitted pathogens in the United States, C. trachomatis and Herpes Simplex Virus-2 (HSV). Data from this tissue culture model indicate that: i) …

Cytotoxic Effects Of Ruthenium Compounds On Human Cancer Cell Lines., Katie Beth Brown

Electronic Theses and Dissertations

Chemotherapy is the most common cancer treatment. Traditionally, platinum-based drugs are used in chemotherapy. More recently, researchers have focused on ruthenium based compounds as a substitute for the platinum compounds. Ruthenium-based compounds appear to be less toxic to healthy cells than traditional platinum-based compounds. In this study, 7 ruthenium-based compounds were tested on HT-29 (colon) and MCF-7 (breast) human cancer cell lines with the specific aim of determining whether or not any of the ruthenium-based compounds exhibited cytotoxic properties. In addition, levels of vascular endothelial growth factor (VEGF) production were tested in supernate from the cancer cells treated with various …

Characterization Of Murine Cardiac Cholinergic Innervation And Its Remodeling In Type 1 Diabetes., Abigail Marie Mabe

Electronic Theses and Dissertations

Murine models have become increasingly popular to study various aspects of cardiovascular diseases due to their ease of genetic manipulation. Unfortunately, there has been little effort put into describing the distribution of autonomic nerves in the mouse heart, making it difficult to compare current findings from clinical and experimental models related to cardiovascular diseases. Furthermore, determination of the requirements for the development of this system and its maintenance in adult mice remains largely unexplored. This study represents the first detailed mapping of cholinergic neuroanatomy of the mouse heart based on immunohistochemical staining using true cholinergic markers. We found cholinergic innervation …

Police Stress: An Examination Of The Effects Of Stress And Coping Strategies., Derrick Kenwright

Electronic Theses and Dissertations

How police officers deal with stress greatly affects how they carry out their daily lives and how they treat family and friends. In this study 2 police departments were issued surveys to see how the police officers experienced stress. Questions on the survey asked the officers about the sources of stress, sources of support, and which methods they used to alleviate the stress. The surveys were given to the respective departments over a period of 2 months, and 132 surveys were returned. The statistical analysis performed showed danger was a factor when examining stress. Administration support was found to be …

Elucidating The Role Of The Α7 Nicotinic Receptor In The Etiology Of Schizophrenia., Michelle Johnson Chandley

Electronic Theses and Dissertations

The α7 subunit of the nicotinic receptor, a ligand gated ion channel with an affinity for nicotine, has long been implicated in the pathophysiology of schizophrenia due to the extremely high rate of smoking within the patient population. However, the exact role of the receptor has never fully been determined. In the following studies, various functions the receptor may assume in disease state are evaluated. There is a strong relationship between the immune system and schizophrenia, with the α7 subunit possibly serving as the link between the two. One of the following studies looks at the possibility of the receptor …

Attitudes Of Agriscience Teachers, Counselors, Administrators, And Students Toward Selected Agriscience Programs In Mississippi, Mary Hitchner Brister

Theses and Dissertations

The purpose of this study was to determine the attitudes of administrators, counselors, agriscience teachers, and students towards the high school agriscience programs in Mississippi. It was also to determine the average scores of students on a criterion-based exam, and to determine if there was a correlation between the attitude scales of the agriscience teachers, counselors, administrators, students, and the student scores on the exam. The study used a descriptive survey and a descriptive-correlational design. Three different surveys were sent out to 356 agriscience teachers, counselors, and administrators at schools with either a Concepts of Agriscience Technology course or an …

The Effect Of Used Broiler Litter On The Growth And Persistence Of Campylobacter, Zachary Thomas Williams

Theses and Dissertations

Litter is a possible source of Campylobacter colonization for broilers as well as contamination of crops when used as fertilizer. A survey of Arkansas broiler litter indicated that Campylobacter recovery rates were higher in pine shavings and rice hulls than sand. Two experiments utilized three types of litter, which were artificially contaminated with Campylobacter. After 24 hours no Campylobacter could be recovered from any sample. Campylobacter growth was also examined for used pine shaving litter in varying conditions: aerobic atmosphere, micro aerobic atmosphere (6% O2), and moisture content. Campylobacter was recovered for all treatments at the initial sampling, and by …

Ecology Of Larra Bicolor (Hymenoptera: Sphecidae) In The Northern Gulf, Cheri Muthirakalayil Abraham

Theses and Dissertations

Mole Crickets (Scapteriscus spp.) are the most destructive pests in southern turf and pasture grasses. In response to extensive losses from mole crickets, Florida formed a task force to identify natural enemies in the native range of these pests. Two parasitic insects, Larra bicolor and Ormia depleta, and Steinernema scapterisci, an entomopathogenic nematode, were imported and released. Of the two insects, only Larra bicolor has spread to other states infested with mole crickets in the southeast. The present study documents the seasonal biology of Larra bicolor in the northern Gulf region, ornamental plants that can be used as nectar sources, …

A Change For The Future: Presidents' And Deans' Real And Ideal Leadership Practices At Community And Junior Colleges In Mississippi, Mildred Stevenson

Theses and Dissertations

The purpose of the study is to examine real vs. ideal leadership practices of administrators according to race, gender, age, experience, and education level years in current position, and years of experience at Mississippi community and junior colleges. The survey instruments were a modified version of the Leadership Practices Inventory-Self (LPI Self) and the Leadership Practices Inventory-Observer (LPI-Observer) originally developed by Kouzes and Posner in 1985 and updated by Kouzes and Posner in 1997. The reliability and validity of the instruments have been consistently high and the instruments have been used in many studies, including doctoral dissertations and other empirical …

Epidemiologic And Economic Study Of Hyperelastosis Cutis/Herda In The Quarter Horse Cutting Industry, Sally Grant Tipton

Theses and Dissertations

Hyperelastosis Cutis (HC) is an autosomal recessive disorder in Quarter Horses. Homozygous (Hr/Hr) horses are affected with fragile, hyperextensible skin that sloughs and scars easily following minor trauma. Heterozygous (N/Hr) horses appear normal, but carry one copy of the gene. Objectives were to determine inbreeding coefficients of affected (Hr/Hr), carrier (N/Hr), and normal (N/N) Quarter Horses, compared to Thoroughbreds, and evaluate economic effects of HC within the cutting horse industry. Of the top cutting horses from 1985 through 2006, 35 were confirmed carriers by either DNA analysis or production of affected offspring. Although 23% of the earnings from the leading …

Nmr Backbone Chemical Shift Assignments Of The Hvdhfr1:Nadph Binary Complex, Karthikeshwar Vangala

Theses and Dissertations

Extremophiles are the organisms that survive in environments which are inhospitable to other creatures. This thesis made an attempt to understand the enviromental effects, in particular saline, on enzyme structure by using three dimensional NMR. The enzyme DHFR1 from halophile Haloferaxi volcanii is complexed with its coactor NADPH, and the saline effects on the complex are studied by comparison with its apoenzyme, hvDHFR1. Backbone chemical shift assignments of the hvDHFR1:NADPH complex were attained which can be functional (along with future work) in understanding the effect of salts on enzyme structure, function, and flexibility. A total of 27 amino acids were …

Nutrient Digestibility And Utilization By Cattle Consuming Cotton Gin Mote As A Replacement For Forage, Cassie Marie Welch

Theses and Dissertations

Two studies were conducted to evaluate cotton gin mote as a forage replacement by determining nutrient digestibility and nitrogen and energy retention. Three diets were used: 0%, 50%, and 100% mote. Dry matter intake was greatest for steers consuming 100% mote. Digestibility of DM, OM, CP, and energy was least for steers consuming 50% mote and greatest for those consuming 0% mote. Digestibility of NDF and ADF was greatest for steers consuming 0% mote. Fat digestibility was greatest by steers consuming 0% mote and least by steers consuming 100% mote, with those consuming 50% mote being intermediate. Steers consuming 0 …

Population Characteristics Of Interior Double-Crested Cormorants Breeding Across The Southern Border Of Ontario, Jennifer Erin Chastant

Theses and Dissertations

Interior Double-crested cormorant reproduction was examined on a large geographical scale to evaluate management actions. Three distinct breeding areas across Ontario were selected. Beginning in 2002, over 11,000 preledged cormorants have been marked. During 2006 and 2007, re-observation of banded birds, colony data such as nest, egg, and chick measurements, and island morphology, were collected. Data revealed no significant regional differences in adult size. However, eggs in eastern Lake Ontario (ELO) were larger than North Channel of Lake Huron (NChan) and Lake of the Woods (LOW). Chicks in ELO throughout development were smaller than NChan and LOW. Number of gulls …

Stability Of Catfish Methyl Esters Under Normal Versus Accelerated Storage Conditions, Saowalee Jongrattananon

Theses and Dissertations

Oxidative storage stability was conducted to determine the optimum antioxidant for the production of catfish methyl esters (CFME) for use as biodiesel. Peroxide value, anisidine value, 2-thiobarbituric reaction substances, acid value, iodine value, UV absorbance, and induction period were measured. Antioxidant, stability parameters changed over a storage time of one year when stored at 25°C. The CFME were not stable to oxidation without the addition of TBHQ. When stored at 25°C, oxidative parameters indicated that CFME was a stable product that met oxidative stability standards when TBHQ was added. When held at 80°C for 28 days, the samples with TBHQ …

Restoring Blackland Prairies In Mississippi: Remnant-Restored Prairie Comparisons And Techniques For Augmenting Forbs, Andrew Clifford Dailey

Theses and Dissertations

One knowledge gap hindering prairie restoration is uncertainty about when a restored prairie communities sufficiently resemble remnant prairie. I surveyed plant communities in remnant prairies, prairies > 5 years post-restoration, and prairies ≤ 5 years post-restoration in Mississippi. Remnants had the greater species richness. Restored prairies had less cover of woody plants and forbs but greatest non-natives. Restored prairies were not similar to remnant prairies (similarity index = 28.9 - 25.9%), primarily because restored prairies had fewer prairie forbs. Thus, restoration may take decades. Transplanting locallyapted prairie forbs into restored prairies may accelerate restoration, but this has not been evaluated adequately. …

Product Formulation And Consumer Acceptability Of Processed Cheese Made With Different Types Of Cocoas And Chocolates & Product Formulation And Quantitative Descriptive Analysis Of Aged Cheddar Cheese With Different Types Of Chocolate Inclusions, Vishal Kumar Royyala

Theses and Dissertations

The objectives of this research were to compare different types of cocoa and chocolates in the formulation of processed chocolate cheese and aged Cheddar cheese, and to evaluate sensory, physical, chemical and quality parameters of each. Four different varieties of chocolates were utilized along with other ingredients to prepare processed chocolate cheeses and were tested for consumer acceptability for their basic and critical attributes. Chocolate chips of four different types and proportions were incorporated into Cheddar cheese curd, ripened for 105 days, and a trained panel evaluated the sensory parameters. Results indicated that processed chocolate cheese made with Bordeaux chocolate …

An Evaluation Of Magnesium Status And Inflammatory Response During The Third Trimester Of Normal Pregnancy And Preeclampsia, Fauzia Asadullah Khan

Theses and Dissertations

Recent reports suggest that preeclampsia is the result of an excessive maternal systemic inflammatory response. The role of magnesium and systemic inflammation in normal pregnancy and preeclampsia needs to be defined. The objectives of this study were to compare (1) demographic and (2) anthropometric characteristics of normal and preeclamptic pregnant women; and (3) to determine and compare serum iMg++ and tMg concentration and the association between inflammatory makers such as TNFα, leptin, adiponectin, IL10, ET-1, triacylglycerol, FFA, serum iMg++ and, tMg concentration of normal and preeclamptic pregnant women during the third trimester of pregnancy. Thirtyfive healthy pregnant and thirtyive preeclamptic …

The Effect Of Policy And Land Use Change On Water Quality In A Coastal Watershed City: An Analysis Of Covington, Louisiana, Kenneth Tyler Langley

Theses and Dissertations

There is currently a great need to expand the understanding of land use policy’s impact on water quality. The purpose of this thesis is to examine local policy in place in Covington, Louisiana in order to find out if it is having an impact on land use, which then has an impact on water quality. Methods used included reclassification of land use categories within a Geographical Information System and analysis of changes in the city over a four year period. Policy was assessed using a pre-existing evaluation method from The Center for Watershed Protection as well as by a more …

Formulation Of A Fruit Slush Using Evaporated Sweet Whey, Reginald Carl Sean Johnson

Theses and Dissertations

Wheyruit slush formulas were evaluated prior to consumer testing of pre-selected formulated beverages. Varying ingredients were prepared in accordance to a factorial design of maximum use of whey and minimum use of additional ingredients. Whey was obtained from the Mississippi State University Dairy Processing Plant and evaporated. The evaporated sweet whey was combined with blueberries, cherry concentrate, Splenda®, water and ice. Sensory tests were conducted to evaluate appearance, flavor and overall acceptability of formulations. Panelists were asked to participate in a survey to elaborate personal perceptions of the products. Formulation of 125mL whey and 30g blueberries had the higher acceptability …

Determination And Compatibility Of Putatively Hypovirulent And Virulent Isolates Of Cryphonectria Parasitica Collected From The Great Smoky Mountains National Park, David Franklin Mcneill

Theses and Dissertations

A two-year study was conducted to collect and identify hypovirulent isolates of the chestnut blight fungus (Cryphonectria parasitica) from American chestnut trees located in the Great Smoky Mountains National Park (GRSM). During the survey, 339 isolates were obtained, 54 of which had abnormal morphological characteristics in culture and 3 of these contained dsRNA. Analysis of vegetative compatibility divided all 339 isolates into 34 distinct groups, 16 of which only contained a single isolate. A total of 19 isolates (compared to 3 controls) containing abnormal cultural morphologies or dsRNA were randomly selected for use in a field trial containing 44 natural …

Determination Of Ricin Content In Castor (Ricinus Communis L.) Tissues And Comparison Of Detoxification Methods, Daniel Joseph Barnes

Theses and Dissertations

Experiments were conducted to test for ricin content in tissue samples from four castor cultivars, developing castor seed, germinating castor seedlings, and chemically and heat treated seed meal. Ricin content of each sample was examined via Western blotting with ricin A-chain specific antibodies. Results indicate that ricin is present solely within castor endosperm and is not present any other tissues. Samples from developing seed and germinating seedlings indicate ricin production begins around day 28 post pollination, and ricin is absent from the seedling 6 days after the onset of radicle emergence. This would seem to indicate that the purpose of …

Long-Term Trophic Shifts Among Fishes After Extensive Modification Of A Southeastern U.S. River System, Matthew E. Roberts

Theses and Dissertations

Regulation of the Upper Tombigbee River and its incorporation into the Tennessee-Tombigbee Waterway has resulted in main-channel flows that differ from the pre-regulation condition. Flows differ in (1) magnitude: higher base flows, damped peak flows, and (2) variability: the river rises and falls faster and the number of reversals has increased. A shift in the trophic ecology of the resident fish assemblage corresponded with the altered hydrology. Assemblage-level trophic plasticity manifested through dietary shifts in species present during both time periods are coupled with changes to the taxonomic structure observed previously. Species representing the contemporary assemblage feed on fewer taxa …