Digital Commons Network^™

Current And Future Status Of Chemistry Collections And Chemistry Libraries At Arl Institutions, Jeremy R. Garritano

Libraries Research Publications

The process of consolidating science and technology branch libraries in academic institutions is not new, but with ease of access to electronic resources, and with shrinking budgets and interdisciplinary research the norm, the idea of consolidation has become more attractive in the past decade. This article reports on the results of a 2005 survey of Association of Research Libraries (ARL) institutions and the status of their chemistry-related library resources and facilities. The past, present, and future of these chemistry collections will be discussed with emphasis on those having consolidated in the last ten years or those who plan to consolidate …

Ice Cream Seminars For Graduate Students: Imparting Chemical Information Literacy, Jeremy R. Garritano

Libraries Research Publications

This article provides information on a chemical information literacy program designed primarily for new graduate students. The full implementation of this program is discussed, including defining its purpose, topics covered, content presented, methods of marketing, and evaluation. The result is a series of voluntary seminars given biweekly throughout the academic year. Seminars are based either around a particular resource or database or are centered on a topical problem that may be addressed using multiple resources. Evaluations show that graduate students are pleased with the seminars, including content covered and the format. Areas for future development and experimentation are also suggested.

Mmm...Vanillin: Reaching Graduate Students Through Ice Cream Seminars, Jeremy R. Garritano

Libraries Faculty and Staff Presentations

While there is no required chem. literature course for graduate students at Purdue University, a series of library ice cream seminars is offered throughout the semester as an alternative. These seminars are targeted toward new graduate students to help them adjust to the wide variety of library resources now available to them. Each seminar is focused on either a particular resource (SciFinder Scholar, Beilstein, etc.) or a topical theme (patents, citation searching, spectral information, etc.). Implementation of the seminar series will be explained as well as content presented and effective marketing methods. Results of evaluations and future directions will also …

Random Forests For Classification In Ecology, D. R. Cutler, Thomas C. Edwards Jr., Karen H. Beard, A. Cutler, K. T. Hess, J. C. Gibson, J. J. Lawler

Wildland Resources Faculty Publications

Classification procedures are some of the most widely used statistical methods in ecology. Random forests (RF) is a new and powerful statistical classifier that is well established in other disciplines but is relatively unknown in ecology. Advantages of RF compared to other statistical classifiers include (1) very high classification accuracy; (2) a novel method of determining variable importance; (3) ability to model complex interactions among predictor variables; (4) flexibility to perform several types of statistical data analysis, including regression, classification, survival analysis, and unsupervised learning; and (5) an algorithm for imputing missing values. We compared the accuracies of RF and …

Spatial Mosaic Evolution Of Snail Defensive Traits, Steve G. Johnson

Biological Sciences Faculty Publications

Background:

Recent models suggest that escalating reciprocal selection among antagonistically interacting species is predicted to occur in areas of higher resource productivity. In a putatively coevolved interaction between a freshwater snail (Mexipyrgus churinceanus) and a molluscivorous cichlid (Herichthys minckleyi ), we examined three components of this interaction: 1) spatial variation in two putative defensive traits, crushing resistance and shell pigmentation; 2) whether abiotic variables or frequency of molariform cichlids are associated with spatial patterns of crushing resistance and shell pigmentation and 3) whether variation in primary productivity accounted forsmall-scale variation in these defensive traits.

Results: Using spatial …

Why Have A Theory Of Law And Technological Change?, Lyria Bennett Moses

Minnesota Journal of Law, Science & Technology

No abstract provided.

Faculty Masthead, Mjlst Editorial Board

Minnesota Journal of Law, Science & Technology

No abstract provided.

Faculty Masthead, Mjlst Editorial Board

Minnesota Journal of Law, Science & Technology

No abstract provided.

Genetic Diversity, Plant Adaptation Regions, And Gene Pools For Switchgrass, Michael D. Casler, Chad A. Stendal, Ludmila Kapich, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Switchgrass (Panicum virgatum L.) is a perennial grass native to the North American tallgrass prairie and broadly adapted to the central and eastern USA. Transfer of germplasm throughout this region creates the potential of contaminating local gene pools with genes that are not native to a locale. The objective of this study was to identify structural patterns and spatial variation for molecular markers of switchgrass populations from the northern and central USA. Forty six prairie-remnant populations and 11 cultivars were analyzed for random amplified polymorphic DNA (RAPD) markers. Although there was significant population differentiation, little of this variation was …

Biology And Management Of Plodia Interpunctella (Lepidoptera: Pyralidae) In Stored Products, S. Mohandass, F. H. Arthur, K. Y. Zhu, James E. Throne

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plodia interpunctella (Hubner), the Indian meal moth, is a world-wide insect pest of stored-products and processed food commodities. It can infest a variety of products and is perhaps the most economically important insect pest of processed food. In this review, we summarize the biology of P. interpunctella, discuss oviposition and development in relation to temperature, environment and food source, examine studies involving sampling and detection, describe various aspects of integrated control, summarize the current knowledge regarding management of P. interpunctella, and address potential areas for new research. The use of reduced-risk insecticides, non-chemical control, targeted pest management through …

Quantification And Comparison Of Metallothionein Levels In Crayfish (Orconectes Propinquus) As A Biomarker Of Metal Pollution In The Central Upper Peninsula Of Michigan, Heather Anne Martin

All NMU Master's Theses

No abstract provided.

Reverse-Phase High Performance Liquid Chromatography Analyses Of Grape (Vitis Vinifera) Seed Extracts For The Presence And Activity Of The Proanthocyanidin Condensing Enzyme, Nagib M. Ward

All NMU Master's Theses

No abstract provided.

Racially Restrictive Covenants In The State Of Washington: A Primer For Practitioners, Rajeev Majumdar

Seattle University Law Review

Part II of this Comment will begin by examining the history of racially restrictive covenants, specifically the nature of covenants and the role of the federal government in both spreading and hindering the usage of such covenants. Part III will discuss the legal underpinnings of what makes such covenants unenforceable in Washington, and the best processes an attorney can use to remove them. Part IV will discuss a recent case that has significantly altered the collateral consequences of attempting to destroy racially restrictive covenants upon other associated covenants. As a result, those seeking to retain the benefits of other covenants …

Fear, Faith And The Fatherland : The Complexities Of Prejudice In The Civil Rights Movement, Kate Medley

Electronic Theses and Dissertations

No abstract provided.

Forage Breeding, Kenneth P. Vogel, Joann F.S. Lamb

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Plant breeding is human-directed evolution. This process developed all major crops and their respective races, strains, or cultivars. Although humans have successfully manipulated the genetic resources of plants for several thousand years, the science of genetics and breeding was not developed until the 20th century. Breeding work on a few forage crops began in the early part of the 20th century (Wilkins and Humphreys, 2003) and was focused mainly on developing strains that had improved establishment, persistence, forage yields, and improved insect and disease resistance. These remain essential attributes of cultivated forages (Burton, 1986). In the last 40 yr, objectives …

Mycobacterial Bacilli Are Metabolically Active During Chronic Tuberculosis In Murine Lungs: Insights From Genome-Wide Transcriptional Profiling, Adel M. Talaat, Sarah K. Ward, Chia-Wei Wu, Elizabeth Rondon, Christine Tavano, John Bannantine, Rick Lyons, Stephen A. Johnston

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chronic tuberculosis represents a major health problem for one-third of the world’s population today. A key question relevant to chronic tuberculosis is the physiological status of Mycobacterium tuberculosis during this important stage of infection. To examine the molecular bases of chronic tuberculosis and the role of host immunity in mycobacterial growth, we determined the mycobacterial transcriptional profiles during chronic and reactivation phases of murine tuberculosis using in vivo microarray analysis (IVMA). Following 28 days of aerosol infection, mycobacterial counts remained stable, although the bacilli were metabolically active with a 50% active transcriptome. The expression of genes involved in lipid and …

Mycotoxin Occurrence And Aspergillus Flavus Soil Propagules In A Corn And Cotton Glyphosate-Resistant Cropping Systems, K. N. Reddy, H. K. Abbas, R. M. Zablotowicz, C. A. Abel, C. H. Koger

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The effects of cotton–corn rotation and glyphosate use on levels of soil-borne Aspergillus flavus, aflatoxin and fumonisin contamination in corn and cotton seed were determined during 2002–2005 in Stoneville, Mississippi (USA). There were four rotation systems (continuous cotton, continuous corn, cotton–corn and corn–cotton) for both glyphosate-resistant (GR) and non-GR cultivars–herbicide system arranged in a randomized complete block design with four replications. Aspergillus flavus populations in surface (5-cm depth) soil, sampled before planting (March/April), mid-season (June) and after harvest (September), ranged from 1.47 to 2.99 log (10) cfu g^-1 soil in the four rotation systems. Propagules of A. flavus …

First Report Of Yersinia Ruckeri Biotype 2 In The Usa, Cova R. Arias, Oscar Olivares-Fuster, Karl Hayden, Craig A. Shoemaker, John M. Grizzle, Phillip H. Klesius

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery-reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri, based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic …

Mineralization Of Nitrogen In Soils Amended With Dairy Manure As Affected By Wetting/Drying Cycles, D. B. Watts, H. A. Torbert, S. A. Prior

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in manure management and its effects on nitrogen (N) mineralization has increased in recent years. The focus of this research was to investigate the N-mineralization rates of different soil types in Coastal Plain soils and compare them to a soil from Illinois. Soils with and without dairy composted manure addition were subjected to different wetting/drying cycles [constant moisture at 60% waterfilled pore space (WFPS) and cycling moisture from 60 to 30% WFPS] under laboratory conditions at three different temperatures (11 °C, 18 °C, and 25 °C). Samples were collected from three different soil types: Catlin (Mollisols), Bama (Ultisols), and …

Non-Composted Municipal Solid Waste Processing Byproduct Effect On Soil Reclamation, H. Allen Torbert, Dick L. Gebhart, Ryan R. Busby, Kenneth N. Potter, Deborah R. Curtain

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A new garbage processing technology has been developed that sterilizes and separates inorganic and organic components of municipal solid waste. The non-composted byproduct of this process, Fluff ®, has the potential to be utilized as a soil amendment to improve soil conditions in highly degraded soils. A study was initiated to evaluate Fluff as a soil amendment for establishing native grasses on disturbed US Army training lands. The Fluff was incorporated into a sandy loam soil at Fort Benning Military Reservation, GA on two sites: a moderately degraded and a highly degraded soil. The Fluff was incorporated at rates of …

Alkaline Phosphatase Reactivity In The Vagina And Uterovaginal Junctionsperm-Storage Tubules Of Turkeys In Egg Production: Implications Forsperm Storage, M. R. Bakst, V. Akuffo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Currently there remains contradictory intortliation on the localisation and possible roleof alkaline phosphatase (AP) in the chicken and Japanese quail oviducts.

2.Using turkeys with a hard-shelled egg in their uteri, vaginal and uterovaginal junction mucosae werestretched and fixed as whole mounts prior to the Imistochemical localisation of All activity.

3.Scattered All reactive cells were observed in the vaginal and uteiovaginal junction surface epitheliaand intense All reactivity of the sperm-storage tubule (SST) epithelium, localised to its apical border.

4.We suggest that such All in hens in egg production may reflect cell dif.ftreiitiation andproliferation in the vagina and SST and possibly …

Adrenomedullin (Am) And Adrenomedullin Binding Protein (Am-Bp) In The Bovine Mammary Gland And Milk: Effects Of Stage Of Lactation And Experimental Intramammary E. Coli Infection, Ted H. Elsasser, Anthony V. Capuco, Thomas J. Caperna, Alfredo Martínez, Frank Cuttitta, Stanislaw Kahl

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Adrenomedullin (AM) has been characterized as an endogenous tissue survival factor and modulator of many inflammatory processes. Because of the increased susceptibility of the mammary gland to infection during the time surrounding parturition in the cow, we investigated how milk and tissue content of AM and its binding protein (AM-BP) might be affected by the stage of lactation and the udder health status. Milk and mammary biopsy samples were obtained from Holstein cows 21 days prior to and at various times after calving to represent the dry period and early and midstages of lactation. Additional cows received an intramammary challenge …

Identification And Characterization Of Aquaporin-9 (Aqp9) In Porcine Hepatic Tissue And Hepatocytes In Monolayer Culture, Thomas J. Caperna, Amy E. Shannon, Mark P. Richards, Wesley M. Garrett, Neil C. Talbot

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Aquaporins (AQPs) are members of a large family of integral membrane proteins involved in the rapid movement of water and neutral solutes across cell membranes. In this study, we have prepared an affinity-purified porcine-specific polyclonal antiserum to AQP9 and have investigated the distribution and expression of AQP9 in pig liver tissue and in hepatocytes in primary culture. Immunocytochemical analysis showed that AQP9 was primarily localized in the membrane structures of hepatocytes and was not associated with intrahepatic bile ducts or blood vessels. Western blot analysis indicated that AQP9 ranged in apparent molecular mass between 27 and 38 kD in whole …

Isolation And Characterization Of Porcine Visceral Endoderm Cell Lines Derived From In Vivo 11-Day Blastocysts, Neil C. Talbot, Le Ann Blomberg, Ayesha Mahmood, Thomas J. Caperna, Wesley M. Garrett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Two porcine cell lines of yolk-sac visceral endoderm, designated as PE-1 and PE-2, were derived from in vivo 11-d porcine blastocysts that were either ovoid (PE-1) or at the early tubular stage of elongation (PE-2). Primary and secondary culture of the cell lines was done on STO feeder cells. The PE-1 and PE-2 cells morphologically resembled visceral endoderm previously cultured from in vivo-derived ovine and equine blastocysts and from in vitro-derived bovine blastocysts. Analysis of the PE-1- and PE-2-conditioned medium by 2D-gel electrophoresis and matrix-assisted laser desorption/ionization-time-of-flightmass spectrometry demonstrated that they produced serum proteins. Reverse transcriptase polymerase chain …

Proteomic And Genetic Analysis Of Glycinin Subunits Of Sixteen Soybean Genotypes, Savithiry Natarajan, Chenping Xu, Hanhong Bae, Bryan A. Bailey, Perry Cregan, Thomas J. Caperna, Wesley M. Garrett, Devanand Luthria

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We investigated proteomic and genomic profiles of glycinin, a family of major storage proteins in 16 different soybean genotypes consisting of four groups including wild soybean (Glycine soja), unimproved cultivated soybean landraces from Asia (G. max), ancestors of N. American soybean (G. max), and modern soybean (G. max) genotypes. We observed considerable variation in all five glycinin subunits, G1, G2 G3, G4 and G5 using proteomics and genetic analysis. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry (MS) analysis showed that the wild genotypes had a range of 25-29 glycinin protein spots that included …

Characterization Of Biological Types Of Cattle (Cycle Vii): Influence Of Postpartum Interval And Estrous Cycle Length On Fertility, R. A. Cushman, M. F. Allan, R. M. Thallman, L. V. Cundiff

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Genetic improvement in reproductive efficiency through selection is difficult because many reproductive traits are binomial and have low heritabilities. Before genetic markers can be generated for fertility in cows, greater characterization of reproductive phenotypes is needed to understand the components of the trait. The current study tested the hypotheses that: 1) breeds vary in postpartum interval to estrus (PPIE) and estrous cycle length, 2) a longer estrous cycle immediately before breeding increased pregnancy rates, and 3) a greater number of cycles before breeding increased conception rates. The postpartum interval to estrus, estrous cycle length, and number of cycles before breeding …

Livestock Management During Drought In The Northern Great Plains. I. A Practical Predictor Of Annual Forage Production, R. E. Kruse, M. W. Tess, R. K. Heitschmidt

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This research addressed the hypothesis that spring precipitation data can be used to detect agricultural drought early in the growing season. The Rangetek range model was used to simulate yearly forage data based on historical precipitation and temperature records from the USDA-ARS Fort Keogh Livestock and Range Research Laboratory (Miles City, MT) and the Agriculture and Agri-Food Canada Manyberries Substation (Lethbridge, AB, Canada). Monthly total precipitation and monthly average maximum and minimum temperatures were used to develop regression equations predicting growing season forage production at the Fort Keogh Laboratory and Manyberries Substation. At Fort Keogh Laboratory, a combination of fall …

Visiting Hour, Richard Morris

English Faculty Scholarship

No abstract provided.

Using Comparative Genomics To Reorder The Human Genome Sequence Into A Virtual Sheep Genome, Brian Dalrymple, Ewen Kirkness, Mikhail Nefedov, Sean Mcwilliam, Abhirami Ratnakumar, Wes Barris, Shaying Zhao, Jyoti Shetty, Jillian Maddox, Margaret O'Grady, Frank Nicholas, Allan Crawford, Tim Smith, Pieter De Jong, John Mcewan, V. Hutton Oddy, Noelle Cockett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Is it possible to construct an accurate and detailed subgene-level map of a genome using bacterial artificial chromosome (BAC) end sequences, a sparse marker map, and the sequences of other genomes?
Results: A sheep BAC library, CHORI-243, was constructed and the BAC end sequences were determined and mapped with high sensitivity and low specificity onto the frameworks of the human, dog, and cow genomes. To maximize genome coverage, the coordinates of all BAC end sequence hits to the cow and dog genomes were also converted to the equivalent human genome coordinates. The 84,624 sheep BACs (about 5.4-fold genome coverage) …

Construction And Characterization Of A Full-Length Cdna Library For The Wheat Stripe Rust Pathogen, Peng Ling, Meinan Wang, Xianming Chen, Kimberly A. Garland-Campbell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi.