Digital Commons Network^™

Mycobacterial Bacilli Are Metabolically Active During Chronic Tuberculosis In Murine Lungs: Insights From Genome-Wide Transcriptional Profiling, Adel M. Talaat, Sarah K. Ward, Chia-Wei Wu, Elizabeth Rondon, Christine Tavano, John Bannantine, Rick Lyons, Stephen A. Johnston

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Chronic tuberculosis represents a major health problem for one-third of the world’s population today. A key question relevant to chronic tuberculosis is the physiological status of Mycobacterium tuberculosis during this important stage of infection. To examine the molecular bases of chronic tuberculosis and the role of host immunity in mycobacterial growth, we determined the mycobacterial transcriptional profiles during chronic and reactivation phases of murine tuberculosis using in vivo microarray analysis (IVMA). Following 28 days of aerosol infection, mycobacterial counts remained stable, although the bacilli were metabolically active with a 50% active transcriptome. The expression of genes involved in lipid and …

Color, Cooking Loss And Textural Properties Of Patties As Affected By Catfish Belly Flap Mince Size And Cooking Method, Jin M. Kim, Bartholomew Green

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Patties were prepared with different sizes of channel catfish belly flap mince. Belly flap meat was minced using four different sizes of openings of plate, mixed with salt, formed, and cooked by two methods, in water and oven. Results indicated no differences (p > 0.05) in Kramer shear break force in the patties cooked by different methods for the same size of mince. As the size of meat particles for patties decreased, hardness and gumminess increased, and color differed from fillet, but Kramer shear break force as well as percent cooking loss decreased (p < 0.05). Patties cooked in an oven showed greater color difference from fillet, percent cooking loss, hardness, and gumminess than those cooked in water for the same size of meat particles (p < 0.05). doi: 10. 1300/J030v 16nO 1_03 [Article copies available for a fee from The Haworth Document Delivery Service: 1-800-HA WORTH. E-mail address: Website: .j

First Report Of Yersinia Ruckeri Biotype 2 In The Usa, Cova R. Arias, Oscar Olivares-Fuster, Karl Hayden, Craig A. Shoemaker, John M. Grizzle, Phillip H. Klesius

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A polyphasic characterization of atypical isolates of Yersinia ruckeri (causative agent of enteric redmouth disease in trout) obtained from hatchery-reared brown trout Salmo trutta in South Carolina was performed. The Y. ruckeri isolates were biochemically and genetically distinct from reference cultures, including the type strain, but were unequivocally ascribed to the species Y. ruckeri, based on API 20E, VITEK, fatty acid methyl ester profiles, and 16S rRNA gene sequencing analysis. These isolates were nonmotile and unable to hydrolyze Tween 20/80 and were therefore classified as Y. ruckeri biotype 2. Genetic fingerprint typing of the isolates via enterobacterial repetitive intergenic …

Mineralization Of Nitrogen In Soils Amended With Dairy Manure As Affected By Wetting/Drying Cycles, D. B. Watts, H. A. Torbert, S. A. Prior

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Interest in manure management and its effects on nitrogen (N) mineralization has increased in recent years. The focus of this research was to investigate the N-mineralization rates of different soil types in Coastal Plain soils and compare them to a soil from Illinois. Soils with and without dairy composted manure addition were subjected to different wetting/drying cycles [constant moisture at 60% waterfilled pore space (WFPS) and cycling moisture from 60 to 30% WFPS] under laboratory conditions at three different temperatures (11 °C, 18 °C, and 25 °C). Samples were collected from three different soil types: Catlin (Mollisols), Bama (Ultisols), and …

Alkaline Phosphatase Reactivity In The Vagina And Uterovaginal Junctionsperm-Storage Tubules Of Turkeys In Egg Production: Implications Forsperm Storage, M. R. Bakst, V. Akuffo

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

1. Currently there remains contradictory intortliation on the localisation and possible roleof alkaline phosphatase (AP) in the chicken and Japanese quail oviducts.

2.Using turkeys with a hard-shelled egg in their uteri, vaginal and uterovaginal junction mucosae werestretched and fixed as whole mounts prior to the Imistochemical localisation of All activity.

3.Scattered All reactive cells were observed in the vaginal and uteiovaginal junction surface epitheliaand intense All reactivity of the sperm-storage tubule (SST) epithelium, localised to its apical border.

4.We suggest that such All in hens in egg production may reflect cell dif.ftreiitiation andproliferation in the vagina and SST and possibly …

Using Comparative Genomics To Reorder The Human Genome Sequence Into A Virtual Sheep Genome, Brian Dalrymple, Ewen Kirkness, Mikhail Nefedov, Sean Mcwilliam, Abhirami Ratnakumar, Wes Barris, Shaying Zhao, Jyoti Shetty, Jillian Maddox, Margaret O'Grady, Frank Nicholas, Allan Crawford, Tim Smith, Pieter De Jong, John Mcewan, V. Hutton Oddy, Noelle Cockett

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Background: Is it possible to construct an accurate and detailed subgene-level map of a genome using bacterial artificial chromosome (BAC) end sequences, a sparse marker map, and the sequences of other genomes?
Results: A sheep BAC library, CHORI-243, was constructed and the BAC end sequences were determined and mapped with high sensitivity and low specificity onto the frameworks of the human, dog, and cow genomes. To maximize genome coverage, the coordinates of all BAC end sequence hits to the cow and dog genomes were also converted to the equivalent human genome coordinates. The 84,624 sheep BACs (about 5.4-fold genome coverage) …

Construction And Characterization Of A Full-Length Cdna Library For The Wheat Stripe Rust Pathogen, Peng Ling, Meinan Wang, Xianming Chen, Kimberly A. Garland-Campbell

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Puccinia striiformis is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi.

Storm Event And Continuous Hydrologic Modeling For Comprehensive And Efficient Watershed Simulations, Deva K. Borah, Jeffrey G. Arnold, Maitreyee Bara, Edward C. Krug, Xin-Zhong Liang

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Based on recent reviews of 11 physically based watershed models, the long-term continuous model soil and water assessment tool (SWAT) and the storm event dynamic watershed simulation model (DWSM) were selected to examine their hydrologic formulations, calibrate, and validate them on the 620 km² watershed of the upper Little Wabash River at Effingham, Ill., and examine their compatibility and benefits of combining them into a more comprehensive and efficient model. Calibration and validation of the SWAT by comparing monthly simulated and observed flows and adjusting the model-assigned resulted in coefficients of determination and Nash–Sutcliffe coefficients for individual years and …

High Water Regime Can Reduce Ammonia Volatilization From Soils Under Potato Production, Guodong Liu, Yuncong Li, A. K. Alva

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

This research was conducted with Biscayne marl soil and Krome gravelly loam from Florida and Quincy fine sand and Warden silt loam from Washington to determine ammonia (NH₃) volatilization at various temperature and soil water regimes. Potassium nitrate (KNO₃), ammonium nitrate (NH₄NO₃), ammonium sulfate [(NH₄)₂SO₄], or urea were applied to the soil at a rate of 75 kg N ha^-1. Soil water regime was maintained at either 20% or 80% of field capacity (FC) and incubated at 11, 20, or 29 °C, which represented …

Nitrogen Contribution Of Peanut Residue To Cotton In A Conservation Tillage System, B. Meso, K. S. Balkcom, C. W. Wood, J. F. Adams

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Leguminous crops, particularly winter annuals, have been utilized in conservation systems to partially meet nitrogen (N) requirements of succeeding summer cash crops. Previous research also highlights the benefits of utilizing summer annual legumes in rotation with non-leguminous crops. This study assessed the N contribution of peanut (Arachis hypogaea L.) residues to a subsequent cotton (Gossypium hirsitum L.) crop in a conservation system on a Dothan sandy loam (fine-loamy, kaolinitic, thermic Plinthic Kandiudults) at Headland, AL during the 2003–2005 growing seasons. Treatments were arranged in a split plot design, with main plots of peanut residue retained or removed from …

Influence Of Soil Moisture On Root Colonization Of Glyphosate-Treated Soybean By Fusarium Species, Nathan E. Means, Robert J. Kremer

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

The widespread use of glyphosate-resistant (GR) cropping systems may impact rhizosphere microbial associations and crop productivity. It was previously reported that glyphosate accumulation in the rhizosphere may stimulate colonization of soybean [Glycine max (L.) Merr.] roots by soilborne Fusarium. Field studies often reveal inconsistent root colonization by Fusarium, especially during growing seasons characterized by contrasting rainfall patterns. Therefore, this study was conducted to determine the impact of different soil moisture contents on root colonization of glyphosate-treated soybean by Fusarium species. Glyphosate (0.84 kg ae ha^-1) was applied to greenhouse-grown glyphosate-resistant (GR) soybean at the two to …

Enrollment, Hiring Trends, And College Recruiting For Industrial Education Courses In Northeastern Wisconsin And Michigan's Upper Peninsula Secondary Schools, Kyle L. Bell

All NMU Master's Theses

No abstract provided.

Improving Test Scores In High School Physics Through The Use Of Journals, Timothy J. Barron

All NMU Master's Theses

No abstract provided.

Beauty Is Better With Deception: Motivation And Competition, Anthony J. Crispigna

All NMU Master's Theses

No abstract provided.

Reciprocity Acollection Of Essays, Shannon M. Cole

All NMU Master's Theses

No abstract provided.

Effects Of Seed Mixture Composition And Cover Crop Usage On Productivity And Growth Of Native Prairie Forbs And Grasses, Kimberly S. Larson

All NMU Master's Theses

No abstract provided.

Walking Wounded, Robin J. Kennedy

All NMU Master's Theses

No abstract provided.

Someone To Watch Over Me: Reflection And Authority In The Writing Center, Michael Mattison

The Writing Center Journal

No abstract provided.

Research Strategies To Reduce Bovine Tuberculosis Transmission From Wildlife To Cattle, Are R. Brentensen, Mike R. Dunbar, Robert G. Mclean

United States Department of Agriculture Wildlife Services: Staff Publications

Bovine tuberculosis (bTB) is a zoonotic disease caused by Mycobaterium bovis, and is transmissible to humans, wildlife and domestic livestock. In the northern Lower Peninsula of Michigan, white-tailed deer (Odocoileus virginianus) serve as a reservoir for the disease and pose a significant threat to domestic cattle and captive cervids. Scientists at USDA, APHIS, Wildlife Services, National Wildlife Research Center have designed a variety of laboratory and field studies aimed at reducing or eliminating bTB infection in cattle by interrupting the transmission of the disease from wildlife reservoirs. These strategies include reducing bTB in deer by delivery of …

Modeling Costs Of Using Ovocontrol G For Managing Nuisance Canada Goose (Branta Canadensis) Populations, Joe N. Caudell, Stephanie A. Shwiff

United States Department of Agriculture Wildlife Services: Staff Publications

OvoControl G is a relatively new product that reduces the hatchability of Canada goose (Branta canadensis) eggs. However, little data is available on the cost of application. We present a model for estimating the cost of application of OvoControl G for managing nuisance Canada goose populations. We found that at low goose densities, fixed labor costs are responsible for a significant portion of the cost. As goose densities increase, these fixed costs become equivalent to, and eventually less than, the costs associated with the purchase of the product. We present several scenarios that managers may employ to further …

International Writing Centers Association Information

The Writing Center Journal

No abstract provided.

Thermo-Mechanical Design Of A 100 Ghz Mmic Power Amplifier Using Flip Chip Technology, Sean Cremin

Theses

This thesis is concerned with the thermo-mechanical design, thermal modelling and temperature profile measurement of a 100 GHz flip chip bump bonded MMIC medium power amplifier.

Flip chip mounting offers superior RF performance in terms of output power, gain, noise figure and bandwidth. The thermal challenge was to dissipate approximately two watts of DC power from the GaAs MMIC (monolithic microwave integrated circuit) through Au (gold) stud bumps which equates to less then 7% of the total surface area of the die. Infrared thermographic images confirmed that the flip chip MMIC amplifier was functional with all eight FET stages on …

Literacy, Technologies, And The 21st Century: Scenarios On Technology, Literacy, And The Making Of Meaning, Kathleen Blake Yancey

Language Arts Journal of Michigan

No abstract provided.

Debunking Instant Messenger Myths: Meeting Student Needs In A Digital Age, Meredith A. Graupner

Language Arts Journal of Michigan

No abstract provided.

Power Of Posting Poetry: Teaching New Literacies, J. Gregory Mcverry

Language Arts Journal of Michigan

No abstract provided.

"The Day I Was Born": The Tale Of Two Student Teachers And A Bungled Internet Research Project, Elizabeth Brockman, Christine Luoma, Shae Potocki

Language Arts Journal of Michigan

No abstract provided.

Improving The Preparation Of Pre-Service Teachers In Real-World Environments, Connie Mietlicki

Language Arts Journal of Michigan

No abstract provided.

My Literary Space: Using Social Networking To Teach Character Analysis, Bethany Erickson, David Knapp, Robert Rozema

Language Arts Journal of Michigan

No abstract provided.

English Teachers: We Need To Hop On The Technology Train, M. P. Cavanaugh

Language Arts Journal of Michigan

No abstract provided.

Y2k +7 And Counting: It's Time To Get All Of Michigan's Students Internet Ready, Rita E. Paye

Language Arts Journal of Michigan

No abstract provided.