Digital Commons Network^™

Bacillus Anthracis Virulent Plasmid Px02 Genes Found In Large Plasmids Of Two Other Bacillus Species, Vicki A. Luna, Debra S. King, K. Kealy Peak, Frank Reeves, Lea Heberlein-Larson, William Veguilla, L. Heller, Kathleen E. Duncan, Andrew C. Cannons, Philip Amuso, Jacqueline Cattani

Bioelectrics Publications

In order to cause the disease anthrax, Bacillus anthracis requires two plasmids, pX01 and pX02, which carry toxin and capsule genes, respectively, that are used as genetic targets in the laboratory detection of the bacterium. Clinical, forensic, and environmental samples that test positive by PCR protocols established by the Centers for Disease Control and Prevention for B. anthracis are considered to be potentially B. anthracis until confirmed by culture and a secondary battery of tests. We report the presence of 10 genes (acpA, capA, capB, capC, capR, capD, IS1627, ORF 48, ORF 61, and repA) and the sequence for the …

Adobe Medicus 2006 4 July-August, Health Sciences Library And Informatics Center

adobe medicus

No abstract provided.

Temperament, Distraction, And Learning In Toddlerhood, Wallace E. Dixon Jr., Brenda J. Salley, Andrea D. Clements

ETSU Faculty Works

The word- and nonword-learning abilities of toddlers were tested under various conditions of environmental distraction, and evaluated with respect to children's temperamental attentional focus. Thirty-nine children and their mothers visited the lab at child age 21-months, where children were exposed to fast-mapping word-learning trials and nonlinguistic sequential learning trials. It was found that both word- and nonword-learning were adversely affected by the presentation of environmental distractions. But it was also found that the effect of the distractions sometimes depended on children's level of attentional focus. Specifically, children high in attentional focus were less affected by environmental distractions than children low …

Book Review: When Corporations Rule The World (Second Edition), The Post Corporate World: Life After Capitalism, Glen Kuecker

History Faculty publications

No abstract provided.

Coordinated Diabrotica Genetics Research: Accelerating Progress On An Urgent Insect Pest Problem, Thomas W. Sappington, Blair D. Siegfried, Thomas Guillemaud

Department of Entomology: Faculty Publications

Diabrotica spp. (western, northern, and Mexican corn rootworms) represent the main pest complex of continuous field corn, Zea mays (L.), in North America. The western corn rootworm, Diabrotica virgifera virgifera LeConte, also has become the main pest of continuous corn in Central and Southeastern Europe since its introduction near Belgrade 15–20 years ago, and it represents a major risk to Western Europe. It has already caused economic losses in Eastern Europe, and Western countries such as France have committed large expenditures for containment and/or eradication.

Rootworm larvae feed on corn roots, and damaged plants are more susceptible to drought and …

Effects Of Aphid (Homoptera) Abundance And Surrounding Vegetation On The Encounter Rate Of Coccinellidae (Coleoptera), Chrysopidae (Neuroptera), And Nabidae (Hemiptera) In Alfalfa, James C. Kriz, Stephen D. Danielson, James R. Brandle, Erin E. Blankenship, Geoff M. Henebry

Department of Entomology: Faculty Publications

Predaceous insect encounter rate was measured in 21 southeast Nebraska alfalfa fields through weekly sweep net sampling during 2002-03. The most frequently encountered predaceous insect families were Coccinellidae (Coleoptera), Nabidae (Hemiptera), and Chrysopidae (Neuroptera). The study used multiple regression analysis to examine the effect of aphid abundance and the surrounding vegetative patch composition on predaceous insect abundance. In 2002, Hippodamia parenthesis Say was encountered more frequently in fields with lower aphid abundances, and Hippodamia convergens Guerin-Meneville, Coccinella septempunctata L., and Nabis americoferus Carayon were encountered more frequently in fields with higher aphid abundances. In 2003, Coleomegilla maculata DeGeer was encountered …

Contributors, Justin Scott-Coe, W. Scott Howard

Reconstruction: Studies in Contemporary Culture

Information about contributors to articles in volume 6 issue 3 of Reconstruction.

Environmental Awareness Through Eco-Visualization: Combining Art And Technology To Promote Sustainability, Tiffany Holmes

Reconstruction: Studies in Contemporary Culture

No abstract provided.

Russification In The Soviet East: Education In Uzbekistan And The Emancipation Of Uzbek Women, 1938-1956, Anna Sochnikova

History Theses & Dissertations

Before the Revolution of 1917, the Uzbek population had a traditional system of religious education, but the literacy level of the Uzbek people was very low. After the Soviets seized power in Uzbekistan, they promoted the development of Uzbek language and culture. In 1937 the Soviet government subjected the entire Uzbek nation to political and ideological instructions. The Russian language became the primary language that dominated many aspects of Uzbek life: party apparatus, science, technologies, state administration, and higher education.

Throughout the Soviet period the majority of children in the rural areas of Uzbekistan attended native schools, while an increasing …

Natriuretic Peptides And Nitric Oxide Stimulate Cgmp Synthesis In Different Cellular Compartments, Leslie A Piggott, Kathryn A Hassell, Zuzana Berkova, Andrew P Morris, Michael Silberbach, Thomas C Rich

Faculty, Staff and Student Publications

Cyclic nucleotide-gated (CNG) channels are a family of ion channels activated by the binding of cyclic nucleotides. Endogenous channels have been used to measure cyclic nucleotide signals in photoreceptor outer segments and olfactory cilia for decades. Here we have investigated the subcellular localization of cGMP signals by monitoring CNG channel activity in response to agonists that activate either particulate or soluble guanylyl cyclase. CNG channels were heterologously expressed in either human embryonic kidney (HEK)-293 cells that stably overexpress a particulate guanylyl cyclase (HEK-NPRA cells), or cultured vascular smooth muscle cells (VSMCs). Atrial natriuretic peptide (ANP) was used to activate the …

Illinois State Magazine, Summer 2006, University Marketing And Communications

Illinois State Magazine (1999-present)

Alumni magazine for Illinois State University

Asynchronous Adenoid Cystic Carcinoma Of The Prostate And Transitional Cell Carcinoma Of The Urinary Bladder, Luma M. Fayyad, Khalaf M. Al-Jader, Belal A. Al-Hawwari

Saudi Medical Journal

Histologic variants of prostatic carcinoma are readily recognized. In this report, we describe a rare variant, adenoid cystic carcinoma, in a 75-year-old man previously diagnosed to have transitional cell carcinoma of the urinary bladder. The diagnosis of adenoid cystic carcinoma was made by the characteristic microscopic features of the tumor morphologically and immunohistochemically. Two months later he was found to have metastatic disease. The patient9s treatment consisted of chemotherapy in combination with prednisone and hormonal therapy. Five and a half months after diagnosis, he died with metastatic disease. Making this case unique is the asynchronous occurrence of this variant with …

Neuroscience Workshops For Fifth-Grade School Children By Undergraduate Students: A University–School Partnership, Judith G. Foy, Marissa Feldman, Edward Lin, Margaret Mahoney, Chelsea Sjoblom

Psychological Science Faculty Works

The National Science Education Standards recommend that science be taught using inquiry-based approaches. Inspired by the Dana Alliance for Brain Initiatives, we examined whether undergraduate students could learn how to conduct field research by teaching elementary school children basic neuroscience concepts in interactive workshops. In an inquiry-based learning experience of their own, undergraduate psychology students working under the close supervision of their instructor designed and provided free, interactive, hour-long workshops focusing on brain structure and function, brain damage and disorders, perception and illusions, and drugs and hormones to fifth-graders from diverse backgrounds, and we assessed the effectiveness of the workshops …

The Nature Of Genetic Engineering And The Uses And Potential Abuses Of Biotechnology, Seth J. Matton

Honors Scholar Theses

Over the past decade the topic of genetic engineering has been has been readily debated in the media, but often these debates consist of political rhetoric and fail to offer objective information on the methods and the potential benefits to human health and their environment. In truth, humans have been manipulating the genomes of organisms for thousands of years, and it has been an evolution of scientific knowledge that has led to the more precise methods of genetic engineering. This paper discusses how scientists utilize natural processes to alter the genetic constituents of both prokaryotic and eukaryotic organisms, benefits to …

Foreword: Sarbanes-Oxley For Science, David Michaels

Law and Contemporary Problems

No abstract provided.

Scientific Secrecy And “Spin”: The Sad, Sleazy Saga Of The Trials Of Remune, Susan Haack

Law and Contemporary Problems

Haack sketches an account of what science is and does that suggests how and why the ways in which scientific work is funded can distort or even block its progress. She puts her theory to work by analyzing the troubled history of the trials--clinical and legal--of Immune Response's AIDS drug, Remune.

Sometimes The Silence Can Be Like The Thunder: Access To Pharmaceutical Data At The Fda, Peter Lurie, Allison Zieve

Law and Contemporary Problems

Those committed to the free exchange of scientific information have long complained about various restrictions on access to the FDA's pharmaceutical data and the resultant restrictions on open discourse. A review of open-government procedures and litigation at the FDA demonstrates that the need for transparency at the agency extend well beyond the reach of any clinical trial registry.

Where Has All The Leaf Litter Gone?...., Charlotte Buehler, Chad Byers, Summer Drake

Annual Student Research Poster Session

What Affects the Amount of Leaf Litter in a Forest?
Decomposition rates of leaf litter?
Abiotic soil conditions (pH, calcium content, other nutrients)?
Abundance of earthworms?
Abundance of millipedes?
Abundance of macroinvertebrates (beetles, crickets, spiders)?
Forest composition (maples, oaks, elms)?
Other abiotic factors (microclimate, microtopography, moisture)?
Leaf chemistry (tannin content, lability, recalcitrance)?
Other biotic factors (bacteria, fungi, mycorrhizae)?

The Constitutional Rights Of Non-Custodial Parents, David D. Meyer

Faculty Scholarship

No abstract provided.

Digging A Little Deeper: Coverage Of Archaeology From The U.S. From 1950 To 2000+ In Discipline-Specific And Subject-Oriented Online Indexes, David C. Tyler, Katharine C. Potter, Susan M. Leach, Jennifer M. Kreifels

University of Nebraska-Lincoln Libraries: Faculty Publications

Librarians, faculty and professional researchers, and students often encounter difficulties in locating pertinent journal articles for the field of archaeology. This article examines the coverage given to ninety-three archaeology journals originating in the United States over a fifty-year interval by twelve discipline-specific and subject-oriented indexes available online. The coverage provided by the individual indexes and some of the larger issues surrounding the coverage of the field itself are discussed, and several recommendations are offered.

Cross-Subtype T-Cell Immune Responses Induced By A Human Immunodeficiency Virus Type 1 Group M Consensus Env Immunogen†0--, Eric A. Weaver, Zhongjing Lu, Zenaido T. Camacho, Fatiha Moukdar, Hua-Xin Liao, Ben-Jiang Ma, Mark Muldoon, James Theiler, Gary J. Nabel, Norman L. Letvin, Bette T. Korber, Beatrice H. Hahn, Barton F. Haynes, Feng Gao

Nebraska Center for Virology: Faculty Publications

The genetic diversity among globally circulating human immunodeficiency virus type 1 (HIV-1) strains is a serious challenge for HIV-1 vaccine design. We have generated a synthetic groupMconsensus env gene (CON6) for induction of cross-subtype immune responses and report here a comparative study of T-cell responses to this and natural strain env immunogens in a murine model. Three different strains of mice were immunized with CON6 as well as subtype A, B, or C env immunogens, using a DNA prime-recombinant vaccinia virus boost strategy. T-cell epitopes were mapped by gamma interferon enzyme-linked immunospot analysis using five overlapping Env peptide sets from …

Effectiveness Of A Motion-Activated Laser Hazing System For Repelling Captive Canada Geese, Scott J. Werner, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Effective management techniques are needed to disperse Canada geese (Branta canadensis) and reduce the human–wildlife conflicts associated with high population densities. We evaluated the effectiveness of a motion-activated laser hazing system for repelling captive Canada geese. The system decreased occupancy of 8 pairs of geese on the treated subplot by 83% during habituation trials. When an additional pair of geese were added to the experiment, occupancy of the treated subplot decreased .92% during each of the 20 nights of the extended habituation test. Avoidance (conditioned during the test) remained ,80% of pretreatment levels during the 2 days immediately following the …

Visualization Of Intracellular Transport Of Vesicular Stomatitis Virus Nucleocapsids In Living Cells, Subash C. Das, Debasis Nayak, You Zhou, Asit K. Pattnaik

School of Veterinary and Biomedical Sciences: Faculty Publications

The phosphoprotein (P) of vesicular stomatitis virus (VSV) is a subunit of the viral RNA polymerase. In previous studies, we demonstrated that insertion of 19 amino acids in the hinge region of the protein had no significant effect on P protein function. In the present study, we inserted full-length enhanced green fluorescent protein (eGFP) in frame into the hinge region of P and show that the fusion protein (PeGFP) is functional in viral genome transcription and replication, albeit with reduced activity. A recombinant vesicular stomatitis virus encoding PeGFP in place of the P protein (VSV-PeGFP), which possessed reduced growth kinetics …

Development Of An Electro-Mechanical System To Identify & Map Adverse Soil Compaction Using Gis&Gps, Santosh Pitla, L. G. Wells

Department of Agricultural and Biological Systems Engineering: Presentations and White Papers

A stand-alone electro-mechanical system with a 32-inch disc coulter was developed and tested to identify soil compaction in a 1-acre field located at the University of Kentucky Animal Research Center (UKARC). The system was evaluated by making four passes in the square grid cell. With the aid of hydraulic actuation, the coulter oscillated between depths of 100mm (4-in) and 330mm (13-in) as it moved forward and recorded the vertical impedance force given by the soil continuously. Forty standard soil cone penetrometer measurements along the diagonals to a depth of 400mm (16-in) were taken and the average cone indices (MPa) at …

Is There A Twenty Third Amino Acid In The Genetic Code?, Alexey V. Lobanov, Gregory V. Kryukov, Dolph L. Hatfield, Vadim Gladyshev

Vadim Gladyshev Publications

The universal genetic code includes 20 common amino acids. In addition, selenocysteine (Sec) and pyrrolysine (Pyl), known as the twenty first and twenty second amino acids, are encoded by UGA and UAG, respectively, which are the codons that usually function as stop signals. The discovery of Sec and Pyl suggested that the genetic code could be further expanded by reprogramming stop codons. To search for the putative twenty third amino acid, we employed various tRNA identification programs that scanned 16 archaeal and 130 bacterial genomes for tRNAs with anticodons corresponding to the three stop signals. Our data suggest that the …

Abundance Of And Floral Herbivory On Exotic Bull Thistle Versus Native Tall Thistle In Western Tallgrass Prairie, Chad P. Andersen, Svata M. Louda

School of Biological Sciences: Faculty Publications

An important, yet poorly quantified mechanism to explain the failure of some exotic species to increase and spread is that indigenous natural enemies provide ecosystem resistance to invasiveness. To evaluate this idea, we hypothesized that spillover of native thistle-feeding floral insect herbivores onto Eurasian bull thistle (Cirsium vulgare) from the prairie native tall thistle (C. altissimum) helps limit bull thistle population growth and spread throughout the western tallgrass prairie region in Nebraska. To test this hypothesis, we quantified both the occurrence of bull thistle, a known invasive species worldwide, and the floral herbivory on it by …

Comparison Of Hybrid Capture And Reverse Transcriptase Polymerase Chain Reaction Methods In Terms Of Diagnosing Human Cytomegalovirus Infection In Patients Following Hematopoietic Stem Cell Transplantation, Arif S. Orsal, Murat Ozsan, Istar Dolapci, Alper Tekeli, Meral Beksac

Saudi Medical Journal

OBJECTIVE: Human cytomegalovirus (CMV) is a life threatening cause of infection among hematopoietic stem cell recipients. Developing reliable methods in detecting the CMV infection is important to identify the patients at risk of CMV infection and disease. The aim of this study was to compare the 2 tests- hybrid capture test, which is routinely used in the diagnosis of CMV infection among hematopoietic stem cell recipients, and reverse transcriptase polymerase chain reaction (RT-PCR) detecting UL21.5 mRNA transcripts of the active virus. METHODS: In this prospective study, a total of 178 blood samples obtained from 35 patients following allogeneic hematopoietic stem …

Rapid Enzyme-Linked Immunosorbent Assay For The Diagnosis Of Human Brucellosis In Surveillance And Clinical Settings In Egypt, Moustafa A. Fadeel, Momtaz O. Wasfy, Guillermo Pimentel, John D. Klena, Francis J. Mahoney, Rana A. Hajjeh

Saudi Medical Journal

OBJECTIVE: To optimize and standardize an enzyme-linked immunosorbent assay (ELISA) for rapid diagnosis of human brucellosis in clinical cases identified during a surveillance study for acute febrile illness (AFI). METHODS: Serum samples from patients presenting with AFI at 13 fever hospitals across Egypt between 1999 and 2003 were kept frozen at NAMRU-3 and used in this study. The assay was evaluated in 5 subject groups: brucellosis cases confirmed by blood culture (group I, n=202) 87% positive by standard tube agglutination test (TA), brucellosis cases exclusively confirmed by TA (group II, n=218), blood cultures from AFI cases positive for bacterial species …

Oxidative Stress In Patients With Pulmonary Tuberculosis, Amir Ghorbanihaghjo, Nadereh Rashtchizadeh, Mohammad Rohbaninoubar, Amirmansour Vatankhah, Abdolnasser Rafi

Saudi Medical Journal

No abstract provided.

Evidence Of Human Metapneumovirus Infection In Jordanian Children, Nasser M. Kaplan, Winifred Dove, Ahmad F. Abu-Zeid, Hiyam E. Shamoon, Sawsan A. Abd-Eldayem, Charles A. Hart

Saudi Medical Journal

No abstract provided.