Digital Commons Network^™

International Shipping Of Fumonisins From Maize Extracts On C18 Sorbent, R. T. Riley, O. A. Torres, E. Palencia

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fumonisins are mycotoxins found in maize. In developing countries, the resources required for analysis are often lacking, and the shipping of maize between countries can be difficult since the importation of plant materials requires permits/ inspection to prevent the entry of pests that frequently infest maize. A simple, safe and legal method for shipping maize extracts to the USA was needed to conduct a survey of fumonisins in Central America. The objective was to develop a method for isolating and shipping maize extracts for fumonisin analysis so as to facilitate a survey of fumonisin exposure. The results indicate that fumonisins …

Improvements In Channel Catfish Growth After Two Generations Of Selection And Comparison Of Performance Traits Among Channel Catfish, Blue Catfish, And Hybrid Catfish Fingerlings In An Aquarium Rack System, Brian C. Small

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

A 9-week growth study was conducted to compare the fingerling performance of two genetic groups of channel catfish Ictalurus punctatus (United States Department of Agriculture [USDA] 103 and USDA303) with that of blue catfish I. furcatus and their hybrid in an aquarium rack system. Fish of approximately 20 g were randomly selected from multifamily populations and acclimated to the aquarium rack system for 2 weeks prior to the start of the 9-week study. Differences in growth indices, feed and protein efficiency, nitrogen retention, whole-body proximate composition, and plasma cortisol levels were determined at the completion of the study. The USDA303 …

Restoration Of Spermatogenesis And Male Fertility By Transplantation Of Dispersed Testicular Cells In The Chicken, Pavel Trefil, Alena Micáková, Jitka Mucksová, Jiń Hejnar, Martin Poplstein, Murray R. Bakst, Jirí Kalina, Jean-Pierre Brillard

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Transplantation of male germ cells into sterilized recipients has been widely used in mammals for conventional breeding and transgenesis purposes. This study presents a workable approach for germ cell transplantation between male chickens. Testicular cells from adult and prepubertal donors were dispersed and transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient seminiferous epithelium up to the production of heterologous sperm in about 50% of transplanted males. In comparison to males transplanted with testicular cell preparations from adult donors, in which the first ejaculates with sperm were …

Revegetation Priorities, D. Terrance Booth, Kenneth P. Vogel

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Revegetation is a needed means of mitigating man-made and natural disturbance. Our current ability to address environmental insults contrasts sharply with that existing when John Muir first sowed the roots of environmental awareness or Aldo Leopold and Hugh H. Bennett inspired a land ethic and a sense of stewardship. We now have considerable revegetation science and experience and—equally important—viable native-seed and revegetation industries expert in repairing environmental damage. Through the National Plant Materials program, related and usually cooperative work within state universities and other entities, and the development of ecological service industries, our society has heeded Leopold’s call to take …

Fumonisin Production In The Maize Pathogen Fusarium Verticillioides: Genetic Basis Of Naturally Occurring Chemical Variation, Robert H. Proctor, Ronald D. Plattner, Anne E. Desjardins, Mark Busman, Robert A. E. Butchko

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Fumonisins are polyketide-derived mycotoxins produced by the maize pathogen Fusarium verticillioides. Previous analyses identified naturally occurring variants of the fungus that are deficient in fumonisin C-10 hydroxylation or that do not produce any fumonisins. In the current study, gene deletion and genetic complementation analyses localized the C-10 hydroxylation deficiency to a cytochrome P450 monooxygenase gene in the fumonisin biosynthetic gene (FUM) cluster. Sequence analysis indicated that the hydroxylation deficiency resulted from a single nucleotide insertion that caused a frame shift in the coding region of the gene. Genetic complementation localized the fumonisinnonproduction phenotype to the polyketide synthase …

Human Emanations And Related Natural Compounds That Inhibit Mosquito Host-Finding Abilities, Ulrich R. Bernier, Daniel Kline, Kenneth H. Posey

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

One mechanism by which the action of semiochemicals can be classified is based on the behavioral impact within or external to the species of interest. As such, one can classify a chemical as one of the following^2,3:

1. Pheromone, if it results in response between insects of the same species
2. Kairomone, if it results in response in another species that benefits the species receiving the chemical cue
3. Allomone, if it results in response in another species that benefits the species releasing the chemical cue

However, the distinctions can be more specific by classification of chemical cues …

Laboratory Evaluation Of Avian Odors For Mosquito (Diptera: Culicidae) Attraction, S. A. Allan, Ulrich R. Bernier, D. L. Kline

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

Attraction of Culex quinquefasciatus Say, Culex tarsalis Coquillett, Culex nigripalpus Theobald, and Aedes aegypti (L.) to avian and other host odors was investigated in a dual-port olfactometer. Although attraction to a human arm was high for Ae. aegypti (>80%) and low for all Culex spp. (<25%), all species responded similarly to a chicken (55.3-73.6%). Responses of Ae. aegypti, Cx. quinquefasciatus, and Cx. nigripalpus to feathers were low (<20%) but greater than to controls. There was no difference in attraction of Cx. tarsalis to feathers or controls. Responses to CO₂ (5 ml/min) were low for all species (<15%) except Cx. tarsalis, which were moderate (24.5%). When feathers were combined with CO2, the resulting attraction was additive or lower than responses …

Spectrum 2006, Saint Mary's College Of California

The Undergraduate Spectrum

No abstract provided.

Talking To Scholars = Scholarly Communication, James A. Van Fleet, K Mcquiston, D Thompson, J Zebrowski

Faculty Conference Papers and Presentations

No abstract provided.

Methods Development And Application Of Two-Dimensional Gel Electrophoresis And Mass Spectrometry In Proteomics, Meena Uma Rajagopal

University of Kentucky Doctoral Dissertations

The development of a highly sensitive ruthenium-based fluorescent staining solution isdescribed in this dissertation. The in-house synthesized ruthenium complex (RuMS)containing both sulfonated and non-sulfonated ligand has detection limit of 1 ng ofprotein that is better than colloidal coomassie, silver and ruthenium complex containingall sulfonated ligands (RuBPS). RuMS stain has 100-fold dynamic range and does notinterfere with subsequent mass spectral identification of proteins. The capability of inhousesynthesis of the staining solution makes it a viable cost-effective alternative to theexpensive commercially available fluorescent stain, Sypro Ruby. The low detection limit,broad linear dynamic range and compatibility with mass spectrometry, make thedevelopment of this …

Molecular Recognition Properties And Kinetic Characterization Of Trans Excision-Splicing Reaction Catalyzed By A Group I Intron-Derived Ribozyme, Joy Sinha

University of Kentucky Doctoral Dissertations

Group I introns belong to a class of large RNAs that catalyze their own excision from precursor RNA through a two-step process called self-splicing reaction. These self-splicing introns have often been converted into ribozymes with the ability site specifically cleave RNA molecules. One such ribozyme, derived from a self-splicing Pneumocystis carinii group I intron, has subsequently been shown to sequence specifically excise a segment from an exogenous RNA transcript through trans excision-splicing reaction.The trans excision-splicing reaction requires that the substrate be cleaved at two positions called the 5' and 3' splice sites. The sequence requirements at these splice sites were …

Nietzsche, Truth, And The Horror Of Existence, Philip J. Kain

Philosophy

This article¹ will argue that at the center of Nietzsche's vision lies his concept of the "terror and horror of existence."² As he puts it in The Birth of Tragedy: King Midas hunted in the forest a long time for the wise Silenus .... When Silenus at last fell into his hands, the king asked what was the best and most desirable of all things for man. Fixed and immovable, the demigod said not a word, till at last, urged by the king, he gave a shrill laugh and broke out into these words: "Oh, wretched ephemeral race, …

A Mathematical Model For The Validation Of Safe Air-Blast Chilling Of Cooked Hams, L. J. Wang, A Amézquita, Curtis L. Weller

Department of Agricultural and Biological Systems Engineering: Faculty Publications

An integrated mathematical model of heat transfer and temperature-dependent bacterial growth was developed to validate the safety of cooked hams during air-blast chilling. Heat transfer through a cooked ham was mathematically modeled and analyzed with a finite element method. Response of bacteria to temperatures was quantitatively described using predictive microbiology. The cumulative effect of temperature history on the bacterial growth was taken into account in the model. For chilling cooked hams from 71°C to 10°C, the maximum error between the predicted and experimental core temperature was within 2.2°C, and the deviation between the predicted and measured total weight losses was …

Development And Evaluation Of A Single-Session Expectancy Challenge Intervention To Reduce Alcohol Use Among Heavy Drinking College Students, Hoyee Lau

Electronic Theses and Dissertations

While overall rates of college student drinking have declined slightly since 1980, extreme forms of drinking are escalating. A comprehensive review of all aspects of alcohol use among college students completed by a panel of scientists and college presidents concluded that very few approaches for dealing with student drinking can be considered empirically validated, and they strongly encouraged additional efforts to develop and validate effective strategies. Expectancy challenge approaches designed to reduce risky drinking through changing key expectancies have been identified as one of the few validated strategies, but this approach has not been developed into a format that is …

2006 Wildflower Of The Year: Spicebush, Lindera Benzoin, W. John Hayden

Biology Faculty Publications

Spicebush is a multistemmed deciduous shrub that grows to a height of one to three meters. Young stems are delicate and may be smooth or finely hairy. Leaves are alternate and simple, with an elliptic to obovate blade that tapers at both the base and apex and is bounded by a smooth margin. Examined closely, the margin will reveal a series of fine hairs that project directly out from the leaf edge. In size, leaves are neither remarkably large nor small; they range from one to six inches in length and up to about two and a half inches wide, …

Genetic And Hybridization Evidence Confirms That A Geographic Population Of Gonatocerus Morrilli (Hymenoptera: Mymaridae) From California Is A New Species: Egg Parasitoids Of The Glassy-Winged Sharpshooter Homalodisca Coagulata (Homoptera: Cicadellidae), Jesse H. De León, Walker A. Jones, Mamoudou Sétamou, David J. W. Morgan

United States Department of Agriculture-Agricultural Research Service / University of Nebraska-Lincoln: Faculty Publications

We investigated the differentiation and reproductive isolation among different geographic populations of Gonatocerus morrilli, egg parasitoids of the glassy-winged sharpshooter (Homalodisca coagulata), to confirm previous observations that there may exist a cryptic species complex or a new species. Two mitochondrial genes [cytochrome oxidase subunits I (COI) and II (COII)] and the internal transcribed spacer region 2 (ITS2) of several individuals per population were sequenced. G. morrilli populations from Texas (TX), Florida (FL), California (CA), and an outgroup (G. ashmeadi) were analyzed. For comparison, a population from Argentina (G. annulicornis) morphologically similar to G. …

The Functional Significance Of Multiple Pax7 Isoforms, Chantel L. Burchill

Theses : Honours

The Pax7 gene is critical for specification of both neurons in the mid-brain and skeletal muscle satellite cells. Several alternate transcripts are transcribed from the single gene. Previous studies have shown that the resultant alternate Pax7 isoforms differ in the structure of their paired domain (a DNA-binding domain that influences target gene selection), yet the functional significance of each isoform for specification of neurogenic and myogenic cell types remains unknown. Although previous studies have identified the presence of multiple alternate Pax7 transcripts in both neurogenic and myogenic cell lines, more research is necessary to understand the functional significance of the …

The Effects Of Light Reduction Treatments On Mobile Epifaunain An Amphibolis Griffithii (Black) Den Hartog Seagrass Ecosystem, Helen Barwick

Theses : Honours

One of the main anthropogenic disturbances to seagrass meadows in Australia is reduction in light availability, through nutrient enrichment or suspended sediments. Dredging can create suspended sediment plumes from the expulsion of particulates into the water column and in tum reduces light penetration to seagrass ecosystems. Preliminary investigations have demonstrated that light reduction for different intensities and durations results in reduced seagrass and epiphytic algae biomass. The main aim of this study was to determine the effects of different intensities and durations of light reduction on epifaunal assemblages in Amphibolis griffithii seagrass meadows in Jurien Bay, Western Australia. This was …

Changes In Demersal Wild Fish Aggregations Beneath A Sea-Cage Fish Farm After The Cessation Of Farming, Fernando Tuya, Pablo Sanchez-Jerez, Tim Dempster, Arturo Boyra, Ricardo Haroun

School of Natural Sciences Publications

Demersal, non-cryptic, wild fish were counted in replicate 100 m2 transects beneath a floating sea-cage fish farm and two nearby sandy locations at Gran Canaria (Canary Islands, eastern Atlantic) four times before and after the cessation of farming. Cessation of farming involved the removal of farmed fish and ceasing of the daily feeding, although farm structures (cages and moorings) remained. A “beyond-BACI” sampling design provided the framework to detect the effect of the cessation of farming, which produced qualitative and quantitative changes in the composition and structure of the fish assemblages beneath the sea-cage fish farm compared with two nearby …

Engaging Diversity Through Brain Hemispheric Orientation (Bho) Among College Students: Some Implications On Their Mathematics Performance., Flerida C. Alvaro

EDU-COM International Conference

This study primarily sought empirical results to be used for a more complicated future research undertaking. This study categorized the students of the College of Teacher Education according to their left, right, or confluent brain hemispheric orientation through a preference scale. Also, these college students were classified with respect to their low, average, or high level of Mathematics performance basing from the grades in Mathematics subjects. Diversity in college students‘ gender, age, and ethnicity were analysed for their effect on the brain hemispheric orientation as well as their effect on their level of Mathematics performance. Likewise, this study investigated the …

Interactive E-Assessments, Geoffrey Crisp

EDU-COM International Conference

It is useful for teachers to reflect on the future formats that will be necessary for assessment tasks. There is currently much discussion concerning the need for immersive and authentic learning environments. Teachers will need to investigate assessments that mix real and virtual environments, testing declarative knowledge and also conditional and procedural capabilities. Teachers will need to contemplate using assessments that measure approaches to problem solving and student responses in terms of efficiency, ethical considerations and the involvement of others. This image of assessment is still sometime away, but will be within reach in the short term. Are academic staff …

Mission (Im-)Possible? Increasing The Participation Of Female Students In University Computing Courses, Iwona Miliszewska, Anne Venables, Grace Tan

EDU-COM International Conference

In 1990 the Australian Federal government set a target for a 40% enrolment rate of females in all non-traditional areas of study including computer science. In view of this target, the low enrolment of females in computer science at Victoria University is a persisting concern: enrolment rates have continued to decline from 30% in the early 1990s to less than 20% in recent years, despite significant attempts to arrest the decline by introducing annual student recruitment events such as school visits, career nights, and University Open Days. This suggested that the issue of encouraging females to study computing needed to …

A Real-Time Virtual Classroom System With Two-Way Communication For Distance Learning At King Mongkut’S University Of Technology Thonburi, Thailand, Surachai Suksakulchai, Chalathip Chunkul

EDU-COM International Conference

Because of high demands for higher education in the provinces of Thailand, the school of Industrial Education and Technology at KMUTT has started off-campus courses since 2002. However, the major problem of operation is distance. To cope with the problem, a real-time virtual classroom system with two-way communication has been developed. The system has to overcome the bandwidth problem, which is the major struggle of web-based learning in Thailand, and to maintain the two-way communication between instructors and students. To cope with those problems, video streaming and live chat systems are used. The technique is the trade-off between two-way and …

Normalizing The Abnormal: Disability In Music And Music Theory, Joseph N. Straus

Publications and Research

The emerging interdisciplinary field of disability studies takes as its subject matter the historical, social, and cultural construction of disability. After a brief introduction to disability studies, this article explores the interconnected histories of disability and music as they are manifested in three theoretical approaches to late eighteenth- and early nineteenth-century Western art music (the musical Formenlehre and the tonal theories of Schoenberg and Schenker) and in three works by Beethoven and Schubert. Around the turn of the nineteenth century in Western Europe, disability began to be understood not as something natural and permanent but rather as a deviation from …

Hydrodynamics And Morphology In The Ems/Dollard Estuary: Review Of Models, Measurements, Scientific Literature, And The Effects Of Changing Conditions, Stefan A. Talke, Huib E. De Swart

Civil and Environmental Engineering Faculty Publications and Presentations

The Ems estuary has constantly changed over the past centuries both from man-made and natural influences. On the time scale of thousands of years, sea level rise has created the estuary and dynamically changed its boundaries. More recently, storm surges created the Dollard sub-basin in the 14th -15th centuries. Beginning in the 16th century, diking and reclamation of land has greatly altered the surface area of the Ems estuary, particularly in the Dollard. These natural and anthropogenic changes to the surface area of the Ems altered the flow patterns of water, the tidal characteristics, and the patterns of sediment deposition …

Ce-Qual-W2: A Two-Dimensional, Laterally Averaged, Hydrodynamic And Water Quality Model, Version 3.5, Thomas M. Cole, Scott A. Wells

Civil and Environmental Engineering Faculty Publications and Presentations

This manual documents the two-dimensional, laterally averaged, hydrodynamic and water quality model CE-QUAL-W2. This manual was prepared in the Environmental Laboratory (EL), us Army Engineer Waterways Experiment Station (WES), Vicksburg, MS. Bonita Niel and Dr. William Roper, CERD-C provided funding for Version 3.1 of the manual under the Numerical Model Maintenance Program. The principal investigator for Version 3.2 of CE-QUAL-W2 and the User Manual was Mr. Thomas M. Cole of the Water Quality and Contaminant Modeling Branch (WQCMB), Environmental Processes and Effects Division (EPED), EL. This report supersedes the Version 3.2 manual. Revisions made in this V3.5 manual were made …

The Effects Of Perceptions On Consumer Acceptance Of Genetically Modified (Gm) Foods, Jae-Hwan Han

LSU Doctoral Dissertations

This study investigates the effects of risk/benefit beliefs on consumer purchase intentions for genetically modified (GM) foods. A random, national, mail survey is conducted. Three main problems are addressed in the study. The first problem addressed is to analyze the relationship between consumers risk/ benefit beliefs regarding GM foods, and their willingness to buy GM crop and meat products. The second problem deals with linkages between a consumer’s risk/benefit beliefs about GM foods and willingness to pay (WTP) for GM foods with a benefit disclosure. The third problem addressed is to test a causal relationship between knowledge and trust toward …

Differential Expression And Detection Of Transcripts In Sweetpotato (Ipomoea Batatas (L.) Lam.) Using Cdna Microarrays, Cecilia E. Mcgregor

LSU Doctoral Dissertations

Microarray protocols were developed for sweetpotato (Ipomoea batatas (L.) Lam.) and then used to study issues of importance in sweetpotato physiology and production. The effect of replication number and image analysis software was compared with results obtained by quantitative real-time PCR. The results indicated that reliable results could be obtained using six replicates and UCSF Spot image analysis software. These methodologies were employed to elucidate aspects of sweetpotato development, physiology and response to virus infection. Storage root formation is the most economically important process in sweetpotato development. Gene expression levels were compared between fibrous and storage roots of the cultivar …

Foreword: Why Open Access To Scholarship Matters, Joe Miller

Scholarly Works

On March 10, 2006, the Lewis & Clark Law Review sponsored a day-long symposium entitled Open Access Publishing and the Future of Legal Scholarship. That gathering led to eight papers that are forthcoming in Volume 10, Issue No. 4, of the Lewis & Clark Law Review. In this short Foreword, I offer some thoughts about why all law professors should take an interest in the movement promoting open access to scholarship. The principal reason, based in current circumstances, is the way that using an open access platform extends one's reach. The aspirational reason is that open access platforms enable us …

Self-Injury: An Analysis Of The Behavior, Causes, And Effective Treatments, Lindsey Huyser

Honors Program Theses

Society has also become obsessed with the quest for a perfect body (Lader & Conterio, 1998). Often, this causes great damage for the self-esteem of women because they are pressured to live up to these high expectations. Self-injury is a way to make women feel better. There has been a rise in women working outside of the home as well, making latchkey children a normal occurrence. Children are often home by themselves when they return from school in today's world, which leaves them with nobody to talk to when problems arise. Self-injury can become a way to heal these issues.