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Characterisation Of Algal Derived Organic Nitrogen, Phillippa Kate Curtis-Jackson

School of Geography, Earth and Environmental Sciences Theses

The molecular level characterisation of compounds comprising the dissolved organic nitrogen (DON) pool is an important task for chemical oceanographers wishing to understand its role in the marine N cycle. Low DON concentrations, problems with saline matrices and a lack of pre-concentration methods have all contributed to DON being ignored and disregarded, until recently. Sub units of DON have been analysed as bulk parameters within which the low molecular weight (LMW; < 1,500 Da) fraction has been proposed to drive regenerated production within the oceans. The largest producers of these compounds are phytoplankton. Dissolved combined amino acids (DCAAs) are proposed to be the largest identifiable component of the LMW fraction. Peptides were chosen as the starting point for this study on DON characterisation, due to their unrivalled detection when analysed via liquid chromatography/ mass spectrometry (LC/MS). A method was developed for the characterisation of low molecular weight peptides, released either actively or passively from the axenically-cultured phytoplankton Phaeodactylum tricornutum. Methods for the separation (high performance liquid chromatography; HPLC), detection (mass spectrometric; MS), de-salination and pre-concentration (solid phase extraction; SPE), of analytes present in the culture water sampled during the period of maximum DON, were developed. The successful chromatographic separation of LMW peptides was achieved using a Phenomenex® Gemini Gig column in conjunction with mobile phases of acidified MeOH and water. The SPE method was developed on a Hamilton PRP-1 column containing a polystyrene divinylbenzene stationary phase (PSDVB). During the extraction process the increased hydrophobicity of the PS-DVB phase was predicted to yield increased recoveries of peptides, when compared to analogous d g phase cartridges. The method was transferred to extraction cartridges (500 mg, StrataX). Further investigations were then carried out into the affects of salt and dissolved organic matter on the extraction process. Samples were prepared by applying the extraction method to 1 L supernatants of culture water samples. The HPLC protocol was used to separate the components of the complex sample prior to data acquisition in both full MS mode and in the data dependent mode (DDMS). Mass spectra and chromatograms of ions unique to the cultured waters were identified. Further instrument manipulation allowed interfering ions to be diverted away from the mass analyser and consequently multistage mass spectrometry (MS²) to be carried out on unique ions that exceeded the threshold intensity of 1 x 105. Unique ions identified in the axenic cultures were two amino acids TYR and a modified structure isomerically/isobarically related to TYR. Seven peptides were partially sequenced. These were H-(VAL, THR, Xxx)-OH, H-(GLN/LYS, ALA, ASN, Xxx)-OH, H-(ASN, ALA, GLU, Xxx)-OH, H-(VAL, GLU, Xxx)-OH, H-(SER, HIS, Xxx)-OH, H- (PRO, XLE,HIS, GLN/LYS, GLY, Xxx)-OH and H-(Xxx, XLE, GLN/LYS, XLE, GLU)- OH. One whole peptide was tentatively proposed as H-TRP-PHE-OH. A significant loss of data was observed between the unique peptide ions identified in the MS analysis and the ions that underwent CID during the DDMS analysis, because they were below the threshold ion count or co-eluted with a more dominant ion. The method development carried out through this study has enabled the successful analysis of samples previously considered incompatible with LC/MS instrumentation. The method can be applied to all aquatic environment samples for both contaminant removal and preconcentration requirements and can be seen as an initial step from which many different avenues of future work may be explored.

Reduce, Refine, Replace: The Failure Of The Three R’S And The Future Of Animal Experimentation, Darian M. Ibrahim

Faculty Publications

No abstract provided.

Review Of Elevator To The Gallows, Michael Adams

Publications and Research

Review of Louis Malle's Elevator to the Gallows: http://www.media-party.com/discland/2006/06/elevator-to-the-gallows.html

Review Of Hail! Hail! Rock 'N' Roll, Michael Adams

Publications and Research

Review of Taylor Hackford's Chuck Berry documentary Hail! Hail! Rock 'n'Roll: http://www.media-party.com/discland/2006/08/chuck-berry-hail-hail-rock-n-roll.html

Review Of I Wake Up Screaming, Michael Adams

Publications and Research

Review of H. Bruce Humberstone's I Wake Up Screaming: http://www.media-party.com/discland/2006/07/i-wake-up-screaming.html

Review Of Macbeth, Michael Adams

Publications and Research

Review of Geoffrey Wright's Macbeth: http://www.media-party.com/discland/2007/12/macbeth-2006.html

Review Of Petulia, Michael Adams

Publications and Research

Review of Richard Lester's Petulia: http://www.media-party.com/discland/2006/06/petulia.html

Review Of The River's Edge, Michael Adams

Publications and Research

Review of Allan Dwan's The River's Edge: http://www.media-party.com/discland/2006/07/the-rivers-edge-1957.html

Worlds Together . . . Words Apart: An Assessment Of The Effectiveness Of Arts-Based Curriculum For Second Language Learners, Stephanie Urso Spina

Publications and Research

The objective of this study is to assess whether authentic arts-based1 curricula facilitate the acquisition of English as a second language (ESL) without sacrificing proficiency in the first language (Spanish). This question is examined theoretically and empirically. First, the use of an arts-based curriculum is positioned within a Vygotskiian framework of learning as reflected in current research. This overview is organized by two themes: 1) the authenticity of the art experience and 2) the emphasis on social interaction and the cognitive mediation among sign systems. Applicable findings from related literature are reviewed and synthesized within each of these themes. Secondly, …

Disclosure And Fair Resolution Of Adverse Events, Carol B. Liebman, Chris Stern Hyman

Faculty Scholarship

The health care system in the United States is in turmoil. Patients are being harmed by too many, often fatal, mistakes. At the same time, physicians and hospitals are trying to cope with a costly medical malpractice crisis. These two crises create a vicious cycle. When something goes wrong in patient care, physicians and hospitals withhold apologies and offer as little information as possible for fear that anything they say may be used against them should patients or family members sue. Family members, in many cases, sue not only to receive compensation for injuries, but also in search of answers …

Censorship And Censureship: Insiders, Outsiders, And The Attack On Bhandarkar Institute, Adheesh Sathaye

Journal of Hindu-Christian Studies

On January 5, 2004, the Bhandarkar Institute, a large Sanskrit manuscript library in Pune, was vandalized because of its involvement in James Laine's controversial study of the Maharashtrian king Shivaji. While most of the manuscripts escaped damage, less fortunate was the academic project of South Asian studies, which now faces some serious questions. if our intellectual pursuits should result in the destruction of the very materials we study, or injury to those who help us to study them, are they worth conducting at all? Or might they be conducted in such a way as to avoid violent reaction? As groundwork …

Inducement As Contributory Copyright Infringement: Metro-Goldwyn-Mayer Studios Inc. V. Grokster, Ltd., Laura A. Heymann

Faculty Publications

No abstract provided.

New Orleans, The Chesapeake, And The Future Of Environmental Assessment: Overcoming The Natural Resources Law Of Unintended Consequences, Erin Ryan

Faculty Publications

No abstract provided.

Law Review Article Placement: Benefit Or Beauty Prize?, Dennis J. Callahan, Neal Devins

Faculty Publications

No abstract provided.

Web Services And Virtual Communities, Qingyu Zhang

Faculty Publications

With the escalating need for global and flexible interactions in an increasingly networked society, virtual communities as a new model are becoming important for virtual communication and collaboration. Virtual community refers to a group of people who interact socially for mutual benefits in a shared cyberspace, and a social network of relationships that provide information and a sense of belongings (de Moor & van den Heuvel, 2004; Schubert & Ginsburg, 2000). Schubert and Ginsburg (2000) describe virtual communities as the union between individuals or organizations who share common values and interests using electronic media to regularly communicate within a shared …

The Media As Participants In The International Legal Process, Monica Hakimi

Faculty Scholarship

We know what we know about current international events through the media. The media (with their instantaneous transmission of images and sound across great distances) inform us of everything from the train bombings in Madrid and London, to human rights abuses in Darfur, to the fall of Saddam Hussein’s Iraq. Yet the media do not simply communicate raw information; they selectively filter, define and give shape to the events that they cover — in terms of what is happening, whether it is appropriate, and how relevant international actors should and do respond. The media thus are the nerves of the …

A Structural Model Of Relationships Between Employee Characteristics, Supervisory Mentoring Behaviors And Job Satisfaction, Florence R. Jinadu

Psychology Theses & Dissertations

Organizational researchers have consistently found that developmental mentoring has a positive effect on employees professional and career success. Some of the benefits that have been cited include increased job satisfaction, increased organizational commitment, and increased self-esteem among mentored employees. In addition to these short-term benefits, developmental mentoring has also been found to positively impact long-term career outcomes in the form of increased compensation and career mobility for mentored employees as compared to non-mentored employees. Traditionally, mentors have been described as influential senior members of an organization who provide career support and developmental opportunities to less experienced employees, who are referred …

Understanding The Nature Of Surgical Excellence Using A Competency Modeling Approach, Hope S. Hanner-Bailey

Psychology Theses & Dissertations

Currently, a thorough description of the knowledge, skills, abilities, and other personal characteristics (KSAOs) that distinguish an exceptional surgeon does not exist. This knowledge is pertinent to the development of selection, training, and performance assessment methods that can be used to develop high performing surgeons. Expert surgeons from around the country were recruited to participate in an interview to discuss the KSAOs (i.e., the competencies) needed to be exceptional in the field. A smaller number of novice surgeons were also interviewed. The expert interview data were distilled into a competency model that consists of ten competencies and patterns within the …

The Architecture Of Inclusion: Advancing Workplace Equity In Higher Education, Susan Sturm

Faculty Scholarship

The path to workplace'equality has become a difficult one to navigate. No one can safely rely upon the strategies developed in the 1960s and 1970s to integrate workplaces. Employers face legal and political challenges both for failing to diversify their workplaces and for diversity efforts to overcome that failure. Civil rights and women's rights advocates battle to hold on to the litigation victories of the past, even as they acknowledge judicial remedies' shrinking availability and limited efficacy in addressing many aspects of current-day equality. Anti-discrimination regulators contend with inadequate resources to carry out their traditional enforcement activities, as well as …

Insights- Nov/Dec. 2006, University Relations Staff

Insights

No abstract provided.

Insights- April 2006, University Relations Staff

Insights

No abstract provided.

Ctl Bulletin- 2006, Ctl Staff

Center for Teaching and Learning Bulletin

Occasional information sheet; dates included are:Feb. 15; Mar. 15; Oct. 15

Insights- Febraury 2006, University Relations Staff

Insights

No abstract provided.

Insights- October 2006, University Relations Staff

Insights

No abstract provided.

Stage Center Theatre Newsletter- Oct. 2006, Ilknur R. Ozgur

Stage Center

No abstract provided.

Neiu Magazine- Winter 2006, Neiu Magazine Staff

NEIU Magazine

No abstract provided.

A Treatment Protocol Utilizing Sensory Integrative Techniques For Treating Self-Mutilation, Nikki Stevens

Occupational Therapy Capstones

“It is estimated that one to two million people in the United States intentionally and repeatedly bruise, cut, burn, mark, scratch and mutilate different parts of their own bodies. This estimate represents only the adolescents and adults who actually seek help for the behavior” (Ferentz, 2002). The reasons for self-mutilation behaviors span across a considerable range from post-traumatic stress disorder to hypersensitivity. The research indicates parallels between children who have been sexually, physically, or emotionally abused and self-mulitlation. Basically, it is an unhealthy coping strategy to deal with overwhelming and intense feelings. The current treatment regime varies and includes: medication, …

Eating Disorder Programming In Four-Year College Recreation Wellness Centers, Tiffany Jean Anderson, Amanda Lynn Gulka

Physical Therapy Scholarly Projects

Eating disorders are more prevalent in the college-age group when compared to the lifetime prevalence. Because of this increased prevalence, college recreation/wellness center staff should be aware of their clients in terms of the increased risk for eating disorders. College wellness/recreation centers, while serving the campus community, also serve the individuals within this community diagnosed with or at-risk for an eating disorder. Through survey research, we show who is employed at wellness/recreation centers, what type of training they receive regarding eating disorder, how this training is provided along with its content, and what method of public awareness on eating disorders …

An Electromyographic Study Of Upper Trapezius, Lower Trapezius, And Serratus Anterior Muscle Activity During Traditional And Modified Muscle Testing Positions, Jace Everett, Mike Fowler, Jason Haak, Robby Luck

Physical Therapy Scholarly Projects

Purpose: The purpose of this study is to assess the muscle activity of the shoulder joint force couple induced by the upper trapezius, lower trapezius, and serratus anterior to determine if they are more effectively recruited during the traditional exercise position of forward shoulder elevation in 145° of shoulder abduction versus a modified position of shoulder external rotation while in 80° of shoulder abduction and 90° of elbow flexion

Methods: Nine participants took part in this randomized study in which EMG data was collected during 4 exercise trials of 10 repetitions each on the right shoulder. EMG activity was monitored …

Variations In Emg Activity In Lower Leg Musculature With Use Of The Biomechanical Ankle Platform System (Baps), Carrie Carpenter, Andrea Lund, Jodi Sailer, Slinde Jessie

Physical Therapy Scholarly Projects

Ankle sprains account for 17% to 20% of all injuries in most sports. Other ankle conditions such as posterior tibialis tendon disorder and Achilles tendinosis are quite common in the nonathletic population and require many of the same interventions. Some of these interventions include range of motion, stretching, strengthening and proprioception. Many studies have shown that strength and proprioceptive training are the intervention of choice for those with lateral ankle instability. Many of these studies have supported the use of BAPS in improving proprioception among individuals with lateral ankle instability, however there is only one study examining the use of …