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Interactions Between The American White Pelican And Aquaculture In The Southeastern United States: An Overview, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

The eastern metapopulation of the American White Pelican ( Pelecanus erythrorhynchos ) breeds mainly in the Northern Great Plains, migrates through the Great Plains and along the Mississippi River and winters in the lower Mississippi River Valley and along the Gulf of Mexico. The production of farm-raised Channel Catfish ( Ictalurus punctatus ) in the southeastern United States has increased dramatically in the last 25 years. In 1990, U.S. Department of Agriculture Wildlife Services offices in Arkansas, Louisiana and Mississippi began receiving complaints concerning the foraging of pelicans in commercial Channel Catfish ponds. Because of the relatively shallow depth and …

Introduction: Biology And Conservation Of The American White Pelican, Daniel W. Anderson, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

Two separate, large-scale management units for the American White Pelican ( Pelecanus erythrorhynchos ) in North America are proposed: the eastern and western metapopulations, separated by the North American Continental Divide. The populations on either side of this barrier are subject to contrasting ecological conditions, movement patterns suggest strong geographic separation and each is likely demographically distinct. Subdivisions within these demographic units need to be more precisely defined in the future. Yet, because of the highly colonial nesting habits of the American White Pelican, conservation can still be effective if directed toward separate breeding colonies. Our intent is to provide …

Developing Relative Abundance Techniques (Rats) For Monitoring Rodent Populations, Desely A. Whisson, Richard M. Engeman, Kellie Collins

United States Department of Agriculture Wildlife Services: Staff Publications

Accurate density estimates of rodents are frequently difficult or cost-prohibitive to determine. Thus, a number of techniques (track plates, monitoring blocks, chew cards/sticks, apple slice index, trapping) are often used to index rodent populations. Theoretically, the sensitivity of these indexing techniques could be improved if they applied continuous measurements rather than simple binary measurements (presence/absence). Development of a relative abundance technique that is simple to apply and sensitive to changes in population density is critical for the operational management of rodent pests. In addition to providing a quantitative measure of abundance with statistical parameters, an indexing technique that does not …

The Impact Of Disease In The American White Pelican In North America, Tonie Rocke, Kathryn Converse, Carol Meteyer, Bob Mclean

United States Department of Agriculture Wildlife Services: Staff Publications

Records of reported die-offs of the American White Pelican ( Pelicanus erythrorhynchos ) held by the U.S. Geological Survey National Wildlife Health Center from 1978 through 2003 indicate that type C botulism (caused by Clostridium botulinum ) was the major cause of mortality. In 1996, over 15,000 birds, including 8,500 American White Pelicans, were estimated to have died from type C botulism at the Salton Sea in California. This was the largest documented die-off of any pelican species and was estimated to represent 15-20% of the western metapopulation. This event was also notable in that it was the first time …

Husbandry Of Wild-Caught Greater Sage-Grouse, Paul Oesterle, Robert Mclean, Michael Dunbar, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

This study reports the first successful husbandry and breeding in captivity of wild-caught greater sage-grouse (Ccntroccrcus urophasianus). In October 2003, 2 1 hatch-year greater sage-grouse were trapped in northwestern Nevada and transported to Fort Collins, Colorado. We held grouse in pens at the United States Department of Agriculture's National Wildlife Research Center for 8 months. We offered a varied diet, including native food items such as sagebrush (Arternisia tridentata and A. tripartita) and yarrow (Achilles millefolium). We housed grouse in a large flight pen and allowed to them freerange as one flock. Mortdlity rate was 16.7%. Several of the grouse …

Stability Of The Tranquilizer Drug Propionylpromazine Hydrochloride In Formulated Products, Thomas M. Primus, Bruce A. Kimball, Jerome Hurley, John J. Johnston, Sherm Blom, Peter J. Savarie

United States Department of Agriculture Wildlife Services: Staff Publications

An analytical method to evaluate propionylpromazine hydrochloride (PPZHCl) in tranquilizer formulations was developed using high-performance liquid chromatography (HPLC). During analysis of aged quality-control samples, a previously unreported chromatographic response was observed at a shorter retention time than PPZHCl. Further investigation of formulations stored in trap tap devices at temperatures ranging from 5 to 40 ◦C during field trials at four different locations confirmed the degradation of the active ingredient. Further investigation using HPLC/tandem mass spectrometry revealed two to five degradates, with the major degradates being oxidation products of the active ingredient, PPZHCl. As PPZHCl formulations must be stable when stored …

Coyote Movements And Social Structure Along A Cryptic Population Genetic Subdivision, Benjamin N. Sacks, Brian R. Mitchell, Christen L. Williams, Holly B. Ernest

United States Department of Agriculture Wildlife Services: Staff Publications

A recent region-wide study determined that the central California coyote ( Canis latrans ) population was genetically subdivided according to habitat bioregions, supporting the hypothesis that coyotes exhibit a dispersal bias toward their natal habitat type. Here, we further investigated this hypothesis using radio-collared coyotes captured on a 150-km 2 study site on the border of (i.e. overlapping) two bioregions (Great Valley and Cascade Mountains). As predicted, most coyotes were assigned (based on a priori genetic criteria) to genetic clusters corresponding to one of these two bioregions. All of those assigned to the Great Valley genetic cluster were caught in …

Gnrh Irnmunocontraception Of Male Cats, Megan K. Ross, Julie K. Levy, Lowell A. Miller, Cynda Crawford, Jerry W. Ritchey, Kathleen A. Fagerstone

United States Department of Agriculture Wildlife Services: Staff Publications

The develop~llenot f nonsurgical co~ltraceptivesf or cats may facilitate population control of the species. The purpose of this study was to investigate the utility of GI&H for iinillu~locontraceptioo~fl male cats. Male cats (11=12) were divided into groups of tlxee and were inunuilized once with 0 (sham), 50, 200, or 400 i g synthetic GI&H coupled to keyhole limpet hernocyanin and combined with a nlycobacterial adjuvant to e~lhance inullunogenicity. GI&H ailtibody titer, serunl testostero~lec oncentration, and scrota1 size were detenniiled monthly. At 6 1110, semen was collected by electroejaculation and testes were examined histologically. GnRH antibodies were detected in all cats …

From The Field: Field Searches Versus Vaginal Implant Translocating Elk Calves, Nathan W. Seward, David S. Maehr, Jonathan W. Gassett, John J. Cox, Jeffrey L. Larkin

United States Department of Agriculture Wildlife Services: Staff Publications

We compared the utility of vaginal implant transmitters (VITs) and field searches for locating elk (Cervus elaphus) calves in southeastern Kentucky during the spring of 2001. Retention of VITs among 40 adult females ranged from 1–276 days (x - =61.6±8.4 [SE] days). Approximately half of the transmitters (n=19) were expelled prematurely or malfunctioned. Two of 37 (5%) transmitters in translocated females performed as designed and led to calf capture. We captured more calves (n=9 in 2001; n=16 in 2002) by searching areas where elk exhibited parturition behavior. We suggest that manufacturers refine the current VIT design to increase its application …

Causes Of Wolf Depredation Increase In Minnesota From 1979-1998, Elizabeth K. Harper, William J. Paul, L. David Mech

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

Wolf (Canis lupus) depredations on livestock in Minnesota have been increasing over the last 20 years. A major explanation cited for this increase is wolf range expansion, but no studies have tested this explanation. Additional reasons could include 1) wolf colonization of new areas within long-existing wolf range, 2) learning by wolves in established range, and 3) increased wolf density. We did not assess increasing wolf density as a factor because estimated wolf density in Minnesota has not increased. To assess how each of the other factors might have affected depredations, we created and analyzed a database of …

Foraging Behavior And Monetary Impact Of Wading Birds At Arkansas Baitfish Farms, Scott J. Werner, J. Brett Harrel, David E. Wooten

United States Department of Agriculture Wildlife Services: Staff Publications

WP rnnrlurted fnraging ob,ervations, food habits studie~, and producer aurveys to determine the foraging behavior and monetary impact of great blue herons Ardca hr.rodia5, great egrets A. alba, and little blue heron5 E m t r a cnurulua lora-g in-e at Arkansas haiffish farms. Although great egrets captured most baitfishlminute, captureslstrike were nearlj identical among the three wadine bird snecies. American eizzard shad Dorosoma crordinnum. eoldfish Carossius nuratul. D - . - and giant water bugs Hemiplera: Beloston~atidaew ere collected from the gastro-intestinal tract of grent blue herons. Only golden shiners ,Votrrni~onuc,~ry soleucur and goldfish were found in great egrets. …

Genome Of Deerpox Virus, C. L. Afonso, Gustavo A. Delhon, E. R. Tuhlman, Z. Lu, A. Zsak, V. M. Becerra, L. Zsak, G. F. Kutish, D. L. Rock

Nebraska Center for Virology: Faculty Publications

Deerpox virus (DPV), an uncharacterized and unclassified member of the Poxviridae, has been isolated from North American free-ranging mule deer (Odocoileus hemionus) exhibiting mucocutaneous disease. Here we report the genomic sequence and comparative analysis of two pathogenic DPV isolates, W-848-83 (W83) and W-1170-84 (W84). The W83 and W84 genomes are 166 and 170 kbp, containing 169 and 170 putative genes, respectively. Nucleotide identity between DPVs is 95% over the central 157 kbp. W83 and W84 share similar gene orders and code for similar replicative, structural, virulence, and host range functions. DPV open reading frames (ORFs) with putative …

Dichotomy In Cross-Clade Reactivity And Neutralization By Hiv-1 Sera: Implications For Active And Passive Immunotherapy, Lisa A. Cavacini, Mark Duval, Ajay Patil, Charles Wood, Kanneth H. Mayer, Ruth M. Ruprecht, Marshall L. Posner

Nebraska Center for Virology: Faculty Publications

The identification of broadly reactive and cross-clade neutralizing antibodies will facilitate the development of a more universally effective vaccine for human immunodeficiency virus (HIV). Antibodies in sera from individuals infected with Clade B HIV bind native primary viral isolates, and virus binding correlates with neutralization and stable clinical disease. In this study, we quantified cross-clade antibody reactivity and neutralization by Clades B and C sera. Primary viral isolates were captured by serum IgG bound to anti-human IgG and quantitated as p24 released by lysis of captured virus. Neutralization was determined using PHA-stimulated PBMC. Clade B antibodies reacted more frequently with …

Cutting Edge: Cd4+Cd25+ Regulatory T Cells Contribute To Gender Differences In Susceptibility To Experimental Autoimmune Encephalomyelitis, Jay Reddy, Hanspeter Waldner, Xingmin Zhang, Zsolt Illes, Kai W. Wucherpfennig, Raymond A. Sobel, Vijay K. Kuchroo

Jay Reddy Publications

Female B10.S mice are highly resistant to proteolipid protein (PLP) 139–151-induced experimental autoimmune encephalomyelitis (EAE) and depletion of PLP 139–151- reactive CD4+CD25+regulatory T (Treg) cells can slightly increase their EAE susceptibility. Although male B10.S mice are moderately susceptible to EAE, we report that depletion of Treg cells in male B10.S mice before immunization with PLP 139–151 renders them highly susceptible to severe EAE with more CNS neutrophil infiltrates than nondepleted controls. Increased susceptibility is associated with an enhanced PLP 139–151-specific T cell response and greater production of IFN-γ, IL-6, and IL-17. Male CD4+CD25+ effector cells depleted of Treg cells proliferate …

Latency-Related Gene Encoded By Bovine Herpesvirus 1 Promotes Virus Growth And Reactivation From Latency In Tonsils Of Infected Calves, Sandra Perez, Melissa Inman, Alan R. Doster, Clinton J. Jones

School of Veterinary and Biomedical Sciences: Faculty Publications

Infection of calves with bovine herpesvirus 1 (BHV-1) results in transient immunosuppression that may lead to bacterium-induced pneumonia and, occasionally, death. Although sensory neurons in the trigeminal ganglia (TG) are the primary site of BHV-1 latency, viral genomes are detected in the tonsils of latently infected calves. Dexamethasone (DEX) consistently induces reactivation from latency, and viral gene expression is detected in TG and tonsils. In sensory neurons of latently infected calves, the latency-related (LR) gene is abundantly expressed and is required for reactivation from latency. In the present study, we compared the abilities of wild-type (wt) BHV-1 and a strain …

Factors Associated With The Presence Of Coliforms In The Feed And Water Of Feedlot Cattle, Michael W. Sanderson, Jan M. Sargeant, David G. Renter, Dicky Dee Griffin, Robert A. Smith

School of Veterinary and Biomedical Sciences: Faculty Publications

The objective of this study was to investigate coliform counts in feedlot cattle water and feed rations and their associations with management, climate, fecal material, and water Escherichia coli O157 using a cross-sectional study design. Coliform counts were performed on feed samples from 671 pens on 70 feedlots and on water samples from 702 pens on 72 feedlots in four U.S. states collected between May and August 2001. Management and climate factors were obtained by survey and observation. Month of sampling (higher in May and June), presence of corn silage in the ration (negative association), temperature of the feed 1 …

Serologic Survey Of Select Infectious Diseases In Coyotes And Raccoons In Nebraska, Richard Bischof, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

To obtain data about select zoonotic and other infectious diseases in free-ranging predators in five ecoregions in Nebraska, sera were collected from 67 coyotes (Canis latrans) and 63 raccoons (Procyon lotor)) from November 2002 through January 2003. For coyotes, antibodies were detected against canine distemper virus (CDV, 61%), Francisella tularensis) (32%), Rickettsia rickettsi) (13%), and flaviviruses (48%). None of the coyote sera had antibodies to Borrelia burgdorferi, Brucella canis), or six serovars of Leptospira interrogans). Because serologic cross-reactivity exists among flaviviruses, 14 sera from flavivirus-positive coyotes were also tested for St. Louis …

A Multilocus Population Genetic Survey Of The Greater Sage-Grouse Across Their Range, S. J. Oyler-Mccance, S.E. Taylor, T. W. Quinn

United States Geological Survey: Staff Publications

The distribution and abundance of the greater sage-grouse (Centrocercus urophasianus) have declined dramatically, and as a result the species has become the focus of conservation efforts. We conducted a range-wide genetic survey of the species which included 46 populations and over 1000 individuals using both mitochondria1 sequence data and data from seven nuclear microsatellites. Nested clade and STRUCTURE analyses revealed that, in general, the greater sage-grouse populations follow an isolation-by-distance model of restricted gene flow. This suggests that movements of the greater sage-grouse are typically among neighboring populations and not across the species, range. This may have important …

Transport Of Chemical And Microbial Compounds From Known Wastewater Discharges: Potential For Use As Indicators Of Human Fecal Contamination, Susan Glassmeyer, Edward Furlong, Dana Kolpin, Jeffrey Cahill, Steven Zaugg, Stephen Werner, Michael Meyer, David Kryak

United States Geological Survey: Staff Publications

The quality of drinking and recreational water is currently (2005) determined using indicator bacteria. However, the culture tests used to analyze for these bacteria require a long time to complete and do not discriminate between human and animal fecal material sources. One complementary approach is to use chemicals found in human wastewater, which would have the advantages of (1) potentially shorter analysis times than the bacterial culture tests and (2) being selected for human-source specificity. At 10 locations, water samples were collected upstream and at two successive points downstream from a waste-waster treatment plant (WWTP); a treated effluent sample was …

West Nile Virus Infection In Farmed American Alligators (Alligator Mississippiensis) In Florida, Elliott R. Jacobson, Pamela E. Ginn, J. Mitchell Troutman, Lisa Farina, Lillian Stark, Kaci Klenk, Kristen L. Burkhalter, Nicholas Komar

United States Department of Agriculture Wildlife Services: Staff Publications

No abstract provided.

Serologic Evidence Of Exposure Of Wild Mammals To Flaviviruses In The Central And Eastern United States, J. Jeffrey Root, Jeffrey S. Hall, Robert G. Mclean, Nicole L. Marlenee, Barry J. Beaty, Justin Gansowski, Larry Clark

United States Department of Agriculture Wildlife Services: Staff Publications

Serosurveys were conducted to obtain flavivirus and West Nile virus (WNV) seroprevalence data from mammals. Sera from 513 small- and medium-sized mammals collected during late summer and fall 2003 from Colorado, Louisiana, New York, Ohio, and Pennsylvania were screened for flavivirus-specific antibodies. Sera samples containing antibody to flaviviruses were screened for WNV-specific antibodies by epitope-blocking enzyme-linked immunosorbent assays and confirmed with plaque reduction neutralization tests. Prevalence of WNV antibodies among study sites ranged from 0% to 42.8% among the mammal communities sampled. High prevalence rates for WNV were noted among raccoons (100%, with a very small sample size, N = …

Climate Change And Amphibians, P.S. Corn

United States Geological Survey: Staff Publications

Amphibian life histories are exceedingly sensitive to temperature and precipitation, and there is good evidence that recent climate change has already resulted in a shift to breeding earlier in the year for some species. There are also suggestions that the recent increase in the occurrence of El Niño events has caused declines of anurans in Central America and is linked to elevated mortality of amphibian embryos in the northwestern United States. However, evidence linking amphibian declines in Central America to climate relies solely on correlations, and the mechanisms underlying the declines are not understood. Connections between embryo mortality and declines …

An Evaluation Of Weather And Disease As Causes Of Decline In Two Populations Of Boreal Toads, Rick D. Scherer, Erin Muths, Barry R. Noon, Paul Stephen Corn

United States Geological Survey: Staff Publications

Two populations of boreal toads (Bufo boreas) experienced drastic declines in abundance in the late 1990s. Evidence supported the hypothesis of disease (the chytrid fungus, Batrachochytrium dendrobatidis) as the cause of these declines. but other hypotheses had not been evaluated. We used an 11-year capture-recapture data set to evaluate weather and disease as causes of these declines. We developed sets of mathematical models that reflected hypothesized relationships between several weather variables and annual survival rates of adult males in these populations. In addition, models that reflected the possibility that the declines were caused by an introduced fungus …

Amphibian Research And Monitoring Initiative (Armi): A Successful Start To A National Program In The United States, Erin Muths, Robin E. Jung, Larissa L. Bailey, Michael J. Adams, P. Stephen Corn, C. Kenneth Dodd Jr., Gary M. Fellers, Walter J. Sadinski, Cecil R. Schwalbe, Susan C. Walls, Robert N. Fisher, Alisa L. Gallant, William A. Battaglin, D. Earl Green

United States Geological Survey: Staff Publications

Most research to assess amphibian declines has focused on local-scale projects on one or a few species. The Amphibian Research and Monitoring Initiative (ARMI) is a national program in the United States mandated by congressional directive and implemented by the U.S. Department of the Interior (specifically the U.S. Geological Survey, USGS). Program goals are to monitor changes in populations of amphibians across U.S. Department of the Interior lands and to address research questions related to amphibian declines using a hierarchical framework of base-, mid- and apex-level monitoring sites. ARMI is currently monitoring 83 amphibian species (29% of species in the …

Marketing Locally Produced Foods: Consumer And Farmer Opinions In Washington County, Nebraska, Mindi L. Schneider, Charles A. Francis

Department of Agronomy and Horticulture: Faculty Publications

Local food system potentials were studied in Washington County, Nebraska, United States. As a departure from most studies of locally based systems, farmers were surveyed in addition to consumers for potential participation. Data about the current food system and opinions and preferences for local production, marketing, and purchasing of food were collected using self-administered mail questionnaires. The response rate was 35% for the farmer survey and 37% for the consumer survey. Results indicated that, on the farming side of the food system, conventional corn and soybean production and marketing predominated in Washington County, and farmer interest in producing for local …

Farmer Research: Conventional Experiences And Guidelines For Alternative Agriculture And Multi-Functional Agro-Ecosystems, Charles S. Wortmann, A. P. Christensen, Keith L. Glewen, Terence A. Hejny, J. Mulliken, J. M. Peterson, D. L. Varner, S. Wortmann, Gary L. Zoubek

Department of Agronomy and Horticulture: Faculty Publications

Crop producers are challenged to operate profitably, use resources efficiently, meet high standards of quality and protect the environment, while sustaining rural economies and societies. Cropping systems are generally fine-tuned and improved through changes that have small effects which can often be verified only through research. The processes and successes of two farmer research projects were studied. Results of these studies, information from other sources and the authors’ reflections on their own experiences were integrated to develop guidelines for the implementation of farmer research projects for alternative agriculture and multi-functional agro-ecosystems with diverse stakeholders. Surveys were mailed to 118 farmers …

Species Diversity And Functional Composition Of Pastures That Vary In Landscape Position And Grazing Management, John A. Guretzky, Kenneth J. Moore, E. Charles Brummer, C. Lee Burras

Department of Agronomy and Horticulture: Faculty Publications

The productivity of grasslands depends in part on their diversity of species and functional composition. Our objective was to examine the effects of three landscape positions (summit, backslope, and toe-slope) and three stocking systems (continuous, rotational, and non-grazed) on species diversity and percentage of cover of grass, legume, and weed species functional types in southeastern Iowa pastures.Data were collected in 0.2-m² plots randomly distributed throughout each of four replicate pastures in spring and summer 2000 and 2001. Backslope landscape positions within pastures managed with either continuous or rotational stocking contained the greatest overall diversity of species. Across years, overall …

Standard Meteorological Measurements, Kenneth Hubbard, Steven E. Hollinger

Department of Agronomy and Horticulture: Faculty Publications

Instruments that measure weather variables have been invented and tested since the time of Leonardo de Vinci. The earliest instruments were crude by today’s standards and required manual observation and notation of the weather variable of interest. In recent years, the miniaturization of circuits–sensors and the use of electronic processors have made it possible to collect ever-increasing numbers of observations on scales not previously considered.

In many agricultural applications, the primary portion of the atmosphere that is of interest is the lower planetary boundary layer, or that layer affected by the earth’s surface. Accurate measurement of weather variables in the …

Crystallization And Preliminary X-Ray Analysis Of Na-Asp-1, A Multi-Domain Pathogenesis-Related-1 Protein From The Human Hookworm Parasite Necator Americanus, Oluwatoyin A. Asojo, Alex Loukas, Mehmet Inan, Rick Barent, Jicai Huang, Brad Plantz, Amber Swanson, Mark Gouthro, Michael M. Meagher, Peter J. Hotez

Department of Chemical and Biomolecular Engineering: Faculty Publications

Human hookworm infection is a major cause of anemia and malnutrition in the developing world. In an effort to control hookworm infection, the Human Hookworm Vaccine Initiative has identified candidate vaccine antigens from the infective larval stage (L3) of the parasite, including a family of pathogenesis-related-1 (PR-1) proteins known as the ancylostoma-secreted proteins (ASPs). The functions of the ASPs are unknown. In addition, it is unclear why some ASPs have one while others have multiple PR-1 domains. There are no known structures of a multi-domain ASP and in an effort to remedy this situation, recombinant Na-ASP-1 has been expressed, …

Evaluating Livestock System Environmental Performance With Whole-Farm Nutrient Balance, Richard K. Koelsch

Department of Agricultural and Biological Systems Engineering: Faculty Publications

As a part of the USEPA’s concentrated animal feeding operation (CAFO) final rule, all CAFOs are required to develop and implement a nutrient management plan (NMP). The USEPA’s emphasis on better management of nutrients appropriately targets a critical environmental issue associated with animal production. The concentration of animals in livestock feeding operations, often separate from feed grain production, requires importing of substantial quantities of feed nutrients. Due to the inefficiencies of nutrient utilization in livestock production, quantities of nitrogen (N) and phosphorus (P) in manure greater than can be utilized in local crop production often result. With the focus of …