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Early Protection Against Pathogenic Virus Infection At A Mucosal Challenge Site After Vaccination With Attenuated Simian Immunodeficiency Virus, Klara Tenner-Ràcz, Christiane Stahl Hennig, Klaus T. Überla

Publications

Atraumatic application of attenuated SIVmac23Δnef vaccine to the tonsils of rhesus macaques provided protection against challenge 26 weeks later with infectious SIVmac251 applied through this route. Early events at the mucosal portal of entry of challenge virus were followed. Wild-type virus was detected in nonvaccinated controls by day 4, and then simian immunodeficiency virus (SIV) replicated vigorously at days 7 and 14. In contrast, a challenge of 10 of 10 vaccinees with SIV did not significantly raise RNA levels in the plasma or increase infected cells in lymphoid tissues, as assessed by single-cell labeling for viral RNA and nef protein. …

In Vivo Targeting Of Antigens To Maturing Dendritic Cells Via The Dec-205 Receptor Improves T Cell Vaccination, Laura C. Bonifaz, David P. Bonnyay, Anna Charalambous

Publications

The prevention and treatment of prevalent infectious diseases and tumors should benefit from improvements in the induction of antigen-specific T cell immunity. To assess the potential of antigen targeting to dendritic cells to improve immunity, we incorporated ovalbumin protein into a monoclonal antibody to the DEC-205 receptor, an endocytic receptor that is abundant on these cells in lymphoid tissues. Simultaneously, we injected agonistic α-CD40 antibody to mature the dendritic cells. We found that a single low dose of antibody-conjugated ovalbumin initiated immunity from the naive CD4⁺ and CD8⁺ T cell repertoire. Unexpectedly, the αDEC-205 antigen conjugates, given s.c., …

Hiv-1-Infected Monocyte-Derived Dendritic Cells Do Not Undergo Maturation But Can Elicit Il-10 Production And T Cell Regulation, Angela Granelli-Piperno, Angelika Golebiowska, Christine Trumpfheller

Publications

Dendritic cells (DCs) undergo maturation during virus infection and thereby become potent stimulators of cell-mediated immunity. HIV-1 replicates in immature DCs, but we now find that infection is not accompanied by many components of maturation in either infected cells or uninfected bystanders. The infected cultures do not develop potent stimulating activity for the mixed leukocyte reaction (MLR), and the DCs producing HIV-1 gag p24 do not express CD83 and DC-lysosome-associated membrane protein maturation markers. If different maturation stimuli are applied to DCs infected with HIV-1, the infected cells selectively fail to mature. When DCs from HIV-1-infected patients are infected and …

Functional Comparison Of The Mouse Dc-Sign, Signr1, Signr3 And Langerin, C-Type Lectins, Kazuhiko Takahara, Yusuke Yashima, Yoshiki Omatsu

Publications

The mouse (m) DC-SIGN family consists of several homologous type II transmembrane proteins located in close proximity on chromosome 8 and having a single carboxyl terminal carbohydrate recognition domain. We first used transfected non-macrophage cell lines to compare the polysaccharide and microbial uptake capacities of three of these lectins - DC-SIGN, SIGNR1 and SIGNR3 - to another homologue mLangerin. Each molecule shares a potential mannose-recognition EPN-motif in its carbohydrate recognition domain. Using an anti-Tag antibody to follow Tag-labeled transfectants, we found that each molecule could be internalized, although the rates differed. However, mDC-SIGN was unable to take up FITC-dextran, FITC-ovalbumin, …

6th Annual Student Academic Conference: Conference Program & Abstracts Volume Vi, Minnesota State University Moorhead

Student Academic Conference Abstract Books

Minnesota State University Moorhead Student Academic Conference abstract book.

Bryn Mawr College Undergraduate College Catalogue And Calendar, 2004-2005, Bryn Mawr College

Bryn Mawr College Calendar

No abstract provided.

The Governor Architecture: Avoiding Catastrophic Forgetting In Robot Learning, Jeremy Strober, Lisa Meeden, Doug Blank

Computer Science Faculty Research and Scholarship

The governor architecture is a new method for avoiding catatrophic forgetting in neural networks that is particularly useful in online robot learn- ing. The governor architecture uses a categorizer to identify events and excise long sequences of repetitive data that cause catastrophic forgetting in neural networks trained on robot-based tasks. We examine the performance of several variations of the governor architecture on a number of re- lated localization tasks using a simulated robot. The results show that governed networks perform far better than ungoverned networks. Governored networks are able to reliably and robustly prevent catastrophic forgetting in robot learning tasks.

Faà Di Bruno’S Formula And Nonhyperbolic Fixed Points Of One-Dimensional Maps, Vadim Ponomarenko

Mathematics Faculty Research

Fixed-point theory of one-dimensional maps of R does not completely address the issue of non-hyperbolic fixed points. This paper generalizes the existing tests to completely classify all such fixed points. To do this, a family of operators are exhibited that are analogous to generalizations of the Schwarzian derivative. In addition, a family of functions f are exhibited such that the MacLaurin series of f(f(x)) and x are identical.

Marc Tessier-Lavigne, 2003, The Rockefeller University

Harvey Society Lectures

Marc Tessier-Lavigne. Wiring the brains: the logic and molecular mechanisms of axon guidance and regeneration

Posted with permission

Gender Identity Disorder : A Misunderstood Diagnosis, Kristopher J. Cook

Theses, Dissertations and Capstones

A transsexual is defined as a person who strongly identifies with the opposite sex. This is also known as gender identity disorder. The individual may identify with the opposite sex to the point of believing that he or she is, in fact, a member of the opposite sex who is trapped in the wrong body. This causes the person to experience serious discomfort with his or her own biological sex. A person with gender identity disorder may or may not know that they actually have a disorder. Not knowing they have this disorder, they can’t explain the problems they experience …

Antimicrobial And Spermicidal Activity Of Hydrogen Peroxide And Nonoxynol-9 Based Gel Preparations, Paul E. Grimmett

Theses, Dissertations and Capstones

Eighty gel formulations were prepared using five buffer systems (0.01M citrate, 0.02M citrate, 0.01M tartrate, 0.02M tartrate, and 0.01M citrate/0.01M tartrate), four hydrogen peroxide (H2O2) concentrations (0%, 0.3%, 0.9%, and 1.2%), and four nonoxynol-9 (N-9) concentrations (0%, 1%, 1.5%, and 2%). The eighty gel formulations were then tested for physio-chemical properties (viscosity and pH), antimicrobial effectiveness (Minimum Inhibitory Concentration Assay) against Candida albicans, Escherichia coli, and Streptococcus agalactiae, and finally, spermicidal effectiveness (Sander Cramer Assay). The gel formulation that contained the best combination of physio-chemical, antimicrobial, and spermicidal properties was a 0.01M citrate buffered hydrogel, which contained 0.9% H2O2 and …

Hk-2 Cells As A Human Model Of Glucuronide Transport, Eliza E. Robertson

Theses, Dissertations and Capstones

Glucuronidation is primarily a pathway of detoxification in most species, but many glucuronide conjugates are associated with toxicity. Numerous drugs are excreted in the urine as glucuronide conjugates. Being organic anions, it is likely that glucuronides are secreted into the urine by organic anion transporters found in renal proximal tubule cells (PTCs). Some drugs that are metabolized by glucuronidation have been shown to cause renal toxicity, yet little is known about the renal handling of glucuronide conjugates. It is hypothesized that glucuronides are transported into renal PTCs by an organic anion transporter (OAT) on the basolateral membrane of the cell. …

Altered Phosphorylation Of [Beta]-Catenin In Glucocorticoid Treated 235-1 Rat Pituitary Tumor Cells, Susie K. Saunders

Theses, Dissertations and Capstones

Beta-catenin is an essential cell adhesion and signaling protein, associated with high prolactin levels in rat pituitary tumor cells. It has been shown that phosphorylation affects the location and activity of b-catenin. Glycogen synthase kinase (GSK3-b) is a serine-threonine kinase that phosphorylates b-catenin on N-terminal residues, targeting it for proteasomal degradation. Studies have shown that C-terminal tyrosine phosphorylation decreases the association of b-catenin with cadherin. In 235-1 rat pituitary tumor cells, our lab has shown that the glucocorticoid analog dexamethasone (Dex) decreases the half- life of b-catenin while increasing the activity of GSK3-b. The current study was undertaken to examine …

The Impact Of Attention Deficit Hyperactivity Disorder (Adhd) On Families: The Perceptions Of Families With An Adhd Child, Patrick Whaley

Theses, Dissertations and Capstones

This study extended the research to include a more complete description o f parents’ perceptions and the impact had on their family functioning. The researcher selected the participants with two categories in mind: (A) Parents must live or not live with an ADHD diagnosed child; (B) Parents will provide their consent for this study. The researcher used the Parenting Stress Index (PSI). The PSI is used to identify stress levels in parent-child systems. By using a t-test for independent samples, the treatment group responded with more stress than the control group (T= 10.143, 28). The researcher found a statistically significant …

The Genetic And Molecular Characterization Of The Polycystic Kidney Disease-Causing Mouse Gene Bicc1, Sarah J. Price

Theses, Dissertations and Capstones

Polycystic kidney disease (PKD) is one of the most common hereditary diseases and is characterized by progressive cyst formation, substantial renal enlargement, and frequently, progression to end-stage renal disease. One way to learn more about the etiology of this disease is to study mouse models that imitate the human situation. The juvenile congenital polycystic kidney disease (jcpk) gene on mouse Chromosome 10 has been found to cause a severe, early onset form of PKD when inherited in an autosomal recessive manner (Flaherty et al., 1995). Previous genetic studies mapped the jcpk locus to a 1 cM region on mouse Chromosome …

The Ecology And Natural History Of The Northern Leopard Frog, Rana Pipiens Schreber, In West Virginia, William Bradley Sutton

Theses, Dissertations and Capstones

The purpose of this study was to gather ecological and life history data for the Northern Leopard Frog, Rana pipiens Schreber, in West Virginia. In Chapter 1, natural history data, such as morphometrics (larval and adult), dorsal coloration, and emergence time were recorded. In Chapter 2, it was discovered that Aeromonas hydrophila and Pseudomonas ssp. skin infections affecting R. pipiens could be identified using BIOLOG analysis. The scope of Chapter 3 was to analyze habitat partitioning between 3 sympatric anurans (R. pipiens, R. catesbeiana, and R. clamitans melanota). The following habitat partitioning gradient was discovered. Rana …

Students’ Assessment Of Biology Education At Marshall University, Chris A. Barker

Theses, Dissertations and Capstones

The purpose of this thesis research was to evaluate the quality of scientific education offered in the College of Science’s Department of Biology at Marshall University. The objectives of this study were to emphasize the important aspects of higher education assessment, point out that a scientifically literate citizenry is imperative for society to function effectively, and to determine what factors contribute to differences among students with regard to their perception of the quality of scientific education they are receiving at Marshall. A survey questionnaire was administered to students in eleven biological science courses during the Fall semester of 2003 and …

Semi-Biosynthesis Of Dna Nanostructures, Aoune Barhoumi

Theses, Dissertations and Capstones

Nanotechnology refers to all technologies aiming to build objects, make measurements, and carry out processes on the nanometer length scale. In particular molecular nanotechnology exemplifies the so-called "bottom up" approach, which is briefly defined as the ability to build useful nanostructures with molecular precision, such as molecular machinery. Such capability for controlling matter at the molecular scale has always been the dream of scientists.

All living things are nanofoundries. Billions of years ago, nature perfectly provided all living things with the most accurate biological nanotechnology systems. Cellular internal dynamics, communicative resonance in protein conformational states, viruses as microreplicators, nanoscale life …

The Use Of Ergogenic Aids Among High School Athletes In Eastern Kentucky, John Robert Brandenburg

Theses, Dissertations and Capstones

A fifteen-question survey was developed to study the use of over-the counter nutrition supplements by high school athletes in eastern Kentucky. Athletic trainers from four local high schools were given one hundred permission forms and surveys. After two weeks only a small number (n=25) completed the requirements to participate in the study. The twenty-five participants consisted of male (n=15) and female (n=10). The athletes with the highest frequency of supplement use were football players (67.7%). The supplements with the highest use were protein powders (n=13) and creatine monohydrate (n=9).

Roles Of Pkc Isozymes In Retinoic Acid-Induced B16 Mouse Melanoma Cell Growth Inhibition And Differentiation, Jianming Han

Theses, Dissertations and Capstones

Protein Kinase C (PKC) has been implicated in many cellular responses. Of the 11 isotypes in this family, seven are expressed in B16 mouse melanoma cells − PKCα, PKCδ, PKCε, PKCθ, PKCλ, PKCζ, and PKCµ.

Treatment of B16 cells with retinoic acid (RA) results in growth inhibition and differentiation. Simultaneously, RA induces a 6-8 fold increase of PKC α protein level, but only about 2-fold increase of PKC enzyme activity. The over- expression of wild type PKCα in B16 cells mimicked the action of RA. The over- expression of enzymatically inactive PKCα, either open or closed conformation, also inhibited B16 …

Docosahexaenoic Acid Modulates Class I Major Histocompatibility Complex Protein Function, Kaleb Marie Hypes

Theses, Dissertations and Capstones

Fatty acids influence immune responses, but how this occurs at the molecular level is not understood. Class I major histocompatibility complex molecules (MHC I), transmembrane proteins required for antigen presentation to T cell receptors, each consist of a 45kDa α chain noncovalently associated with a 12-kDa β₂-microglobulin (β₂m) molecule. Docosahexaenoic acid’s (DHA) effect on the binding of β₂m and anti-MHC I monoclonal antibodies (Mab) was measured. EL4 and RMA-S cells were cultured 48 hours with 0-45µM DHA or oleic acid. Murine to human β₂m exchange and Mab binding were assessed by fluorescence …

Urban Development And Effects To Transportation Systems Of Lexington, Fayette County, Kentucky, Wannaporn Jarupath

Theses, Dissertations and Capstones

Urban growth and its relationship to the transportation system of Lexington, Kentucky were investigated using temporal images of Landsat Thematic Mapper and multi-date line data. Land cover of the area was extracted using Remote Sensing and Geographic Information Systems Technologies. Change detection techniques were employed to identify areas of transformation between 1988 and 2000. Several digital image processes such as geometric registration, radiometric normalization, unsupervised classification, and accuracy assessment were applied to analyze the results. Vector analysis was utilized as well along with raster analysis to examine the effect of urban growth to the transportation system. Two datasets of line …

No Child Left Behind: Flowers Don’T Grow In The Desert, William T. Armaline, D Levy

Faculty Publications

No abstract provided.

Distributing The Costs Of Environmental, Health, And Safety Protection: The Feasibility Principle, Cost-Benefit Analysis, And Regulatory Reform, David M. Driesen

College of Law - Faculty Scholarship

No abstract provided.

Natural Law And The Regulation Of Sexuality: A Critique, Dr. Brent L. Pickett

Richmond Journal of Law and the Public Interest

I will argue that the natural-law understanding of sexuality, and its application to the law, is deeply flawed, in regards to homosexuality and sodomy. I begin by laying out some of the foundations of the natural law position. Central to the position is an account of human goods that are seen as good in themselves, and hence as rational bases for choice and human action. In regards to sexuality, the two most important goods, at least from the natural law perspective, are those of marriage and personal integration. I argue that a real appreciation of the role of these two …

The Japanese Biological And Chemical Warfare Program In China, 1932-1945, Beata Marzena Wilk

Theses

No abstract provided.

Interrupting Heteronormativity: Lesbian, Gay, Bisexual, And Transgender Pedagogy And Responsible Teaching At Syracuse University, Kathleen Farrell, Nisha Gupta, Mary Queen

Books

No abstract provided.

Caenorhabditis Elegans Atx-2 Promotes Germline Proliferation And The Oocyte Fate, Eleanor M. Maine, Dave Hansen, Deborah Springer, Valarie E. Vought

Biology - All Scholarship

In the Caenorhabditis elegans germline, proliferation is induced by Notch-type signaling. Entry of germ cells into meiosis is triggered by activity of the GLD-1 and GLD-2 pathways, which function redundantly to promote meiosis and/or inhibit proliferation. Activation of the germline Notch-type receptor, GLP-1, ultimately inhibits the activities of the GLD-1 and GLD-2 pathways. We previously identified several ego (enhancer of glp-1) genes that promote germline proliferation and interact genetically with the GLP-1 signaling pathway. Here, we show that atx-2 is an ego gene. Our data suggest that ATX-2 is not a positive regulator of the GLP-1 signaling pathway and GLP-1 …

2004 Graduate Bulletin

Graduate Bulletins

After 2003 the University of Dayton Bulletin went exclusively online. This copy was printed from the web and scanned by the Registrar’s Office. For general information about the university please see the Undergraduate Bulletin.

How Does The Use Of Children's Literature Support Student Construction Of Basic Biological Concepts?, Dennis J. Mucenski

Mathematical and Computing Sciences Masters

Children's literature can be defined as a "specific picture books, which are a type of tradebook that contain thirty pages with pictures on every page. Both text and illustrations in a picture book work together to create meaning"(Giorgis & Hartman. 2000, 34). This topic piqued my interest because I am a visual learner and look to the pictures or graphic displays in the text to help explain the text. I find it difficult to read long stretches of text without accompanying graphics or illustrations. But, since it was the end of the school year the idea came and went without …