Digital Commons Network^™

Transgenic Animal And Methods, Lars Nilsson, Huntington Potter, Gary W. Arendash

USF Patents

A transgenic animal, preferably a mouse, that expresses human antichymotrypsin (ACT) in brain tissues is provided, together with animal tissue-derived cell lines and progeny animals of said transgenic animal. Progeny are obtained by mating the transgeny animal with select animal strains used as models of Alzheimer's disease, related neurological disorders, or amyloidogenic diseases. Methods utilizing the parent and progeny animals and cells derived therefrom are disclosed for testing compounds for use as anti-inflammatory drugs, inhibitors of amyloidogenesis, and/or inhibitors of tau protein pathology associated with Alzheimer's disease, in the treatment of a variety of neurological diseases.

Binding Between The Niemann–Pick C1 Protein And A Photoactivatable Cholesterol Analog Requires A Functional Sterol-Sensing Domain, Nobutaka Ohgami, Dennis C. Ko, Matthew Thomas, Matthew P. Scott, Catherine C. Y. Chang, Ta-Yuan Chang

Dartmouth Scholarship

Niemann-Pick type C (NPC) 1 protein plays important roles in moving cholesterol and other lipids out of late endosomes by means of vesicular trafficking, but it is not known whether NPC1 directly interacts with cholesterol. We performed photoaffinity labeling of intact cells expressing fluorescent protein (FP)-tagged NPC1 by using [(3)H]7,7-azocholestanol ([(3)H]AC). After immunoprecipitation, (3)H-labeled NPC1-GFP appeared as a single band. Including excess unlabeled sterol to the labeling reaction significantly diminished the labeling. Altering the NPC1 sterol-sensing domain (SSD) with loss-of-function mutations (P692S and Y635C) severely reduced the extent of labeling. To further demonstrate the specificity of labeling, we show that …

The Bg News August 24, 2004, Bowling Green State University

BG News (Student Newspaper)

The BGSU campus student newspaper. August 24, 2004. Volume 95-Issue 3.

The Caenorhabditis Elegans F-Box Protein Sel-10 Promotes Female Development And May Target Fem-1 And Fem-3 For Degradation By The Proteasome, Sibylle Jager, Hillel T. Schwartz, H. Robert Horvitz, Barbara Conradt

Dartmouth Scholarship

The Caenorhabditis elegans F-box protein SEL-10 and its human homolog have been proposed to regulate LIN-12 Notch signaling by targeting for ubiquitin-mediated proteasomal degradation LIN-12 Notch proteins and SEL-12 PS1 presenilins, the latter of which have been implicated in Alzheimer's disease. We found that sel-10 is the same gene as egl-41, which previously had been defined by gain-of-function mutations that semidominantly cause masculinization of the hermaphrodite soma. Our results demonstrate that mutations causing loss-of-function of sel-10 also have masculinizing activity, indicating that sel-10 functions to promote female development. Genetically, sel-10 acts upstream of the genes fem-1, fem-2, and fem-3 and …

Examining Multisystem Coordination In Response To Stressors In Early Childhood, Tiffany Phu

Electronic Theses and Dissertations

This dissertation presents a set of two studies that investigate linkages between the Hypothalamic-Pituitary-Adrenal (HPA) axis and Sympathetic Nervous System (SNS) in response to a stressor paradigm among young children experiencing low-income. Contextual/child factors that shape multisystem physiologic coordination were also examined. A novel application of structural equation modeling was used to model sample-by-sample linkages between salivary analytes that indexed HPA axis and SNS functioning (respectively, salivary cortisol and salivary alpha amylase [sAA]). The preschoolers in Study 1 showed linkages between salivary cortisol and sAA across a novel research visit at their childcare center that included a stressor paradigm. Child …

Biophotonic Properties Of Cells And Mitochondria, Paul L. Gourley

Light Activated Tissue Regeneration and Therapy

No abstract provided.

Deafdigest: Deaf Sports News August 23, 2004, Barry Strassler

DeafDigest Sports 2004

No abstract provided.

Mitochondrial Metabolism And The Responses To Cellular Injury, Robert K. Naviaux

Light Activated Tissue Regeneration and Therapy

No abstract provided.

Murray Ledger And Times, August 23, 2004, Murray Ledger And Times

The Murray Ledger & Times

No abstract provided.

The Collegian (2004-08-23), Sonia Mejia

UTB/TSC Archives - The Collegian [Newspaper]

Vol. 57, No. 1

August 23, 2004 Reporter, University Of Alabama At Birmingham

eReporter (campus newsletter)

No abstract provided.

Chloroplast Dna Rearrangements In Campanulaceae: Phylogenetic Utility Of Highly Rearranged Genomes, Mary E. Cosner, Linda A. Raubeson, Robert K. Jansen

All Faculty Scholarship for the College of the Sciences

Background

The Campanulaceae (the "hare bell" or "bellflower" family) is a derived angiosperm family comprised of about 600 species treated in 35 to 55 genera. Taxonomic treatments vary widely and little phylogenetic work has been done in the family. Gene order in the chloroplast genome usually varies little among vascular plants. However, chloroplast genomes of Campanulaceae represent an exception and phylogenetic analyses solely based on chloroplast rearrangement characters support a reasonably well-resolved tree.

Results

Chloroplast DNA physical maps were constructed for eighteen representatives of the family. So many gene order changes have occurred among the genomes that characterizing individual mutational …

Monitor Newsletter August 23, 2004, Bowling Green State University

Monitor

Official Publication of Bowling Green State University for Faculty and Staff

Durable Cytotoxic Immune Responses Against Gp120 Elicited By Recombinant Sv40 Vectors Encoding Hiv-1 Gp120 +/- Il-15., Hayley J Mckee, Patricia Y T'Sao, Maria Vera, Puri Fortes, David S Strayer

Department of Pathology, Anatomy, and Cell Biology Faculty Papers

BACKGROUND: A vaccine that elicits durable, powerful anti-HIV immunity remains an elusive goal. In these studies we tested whether multiple treatments with viral vector-delivered HIV envelope antigen (gp120), with and without IL-15, could help to approach that goal. For this purpose, we used recombinant Tag-deleted SV40-derived vectors (rSV40s), since they do not elicit neutralizing antibody responses, and so can be given multiply without loss of transduction efficiency. METHODS: SV(gp120) carried the coding sequences for HIV-1NL4-3 Env, and SV(mIL-15) carried the cDNA for mouse IL-15. Singly, and in combination, these two vectors were given monthly to BALB/cJ mice. Cytotoxic immunity and …

Intersexuality And Universal Marriage, Michael L. Rosin

ExpressO

The proposed Federal Marriage Amendment would raise to the constitutional level the traditional understanding of marriage as the union of one woman and one man. In so doing it would raise to the constitutional level the questions of who is a woman and who is a man. There is currently no settled case or statute law answering these questions. A 1979 Australian annulment case declaring a husband with XX sex chromosomes to be neither a man nor a woman demonstrates the law’s inability to deal with the physically intersexed. Legal scholars defending the traditional view of marriage cite 19th century …

The Chanticleer, 2004-08-20, Coastal Carolina University

The Chanticleer Student Newspaper

The editorially independent student produced weekly newspaper of Coastal Carolina University.

Monographic Acquisitions And Copy Cataloging (Macc) Annual Report 2003-2004, Karen M. Ramsay

Monographic Acquisitions and Copy Cataloging Reports and Documents

Annual report of the Monographic Acquisitions and Copy Cataloging (MACC) Unit, University Library, University of Rhode Island. Covers staffing, hardware and software, monograph acquisitions, and inventory project during the fiscal year 2003-2004.

The Fad-Shielding Residue Phe1395 Regulates Neuronal Nitric-Oxide Synthase Catalysis By Controlling Nadp+ Affinity And A Conformational Equilibrium Within The Flavoprotein Domain, David Konas, Keng Zhu, Manisha Sharma, Kulwant S. Aulak, Gary W. Brudvig, Dennis J. Stuehr

Department of Chemistry and Biochemistry Faculty Scholarship and Creative Works

Phe1395 stacks parallel to the FAD isoalloxazine ring in neuronal nitric-oxide synthase (nNOS) and is representative of conserved aromatic amino acids found in structurally related flavoproteins. This laboratory previously showed that Phe1395 was required to obtain the electron transfer properties and calmodulin (CaM) response normally observed in wild-type nNOS. Here we characterized the F1395S mutant of the nNOS flavoprotein domain (nNOSr) regarding its physical properties, NADP+ binding characteristics, flavin reduction kinetics, steady-state and pre-steady-state cytochrome c reduction kinetics, and ability to shield its FMN cofactor in response to CaM or NADP(H) binding. F1395S nNOSr bound NADP+ with 65% more of …

Chen V. Atty Gen Usa

2004 Decisions

Agency

Dose Threshold For Clinical Success In Coronary Brachytherapy: A Nested Case-Control Study, Harsimran Singh

Yale Medicine Thesis Digital Library

Intravascular brachytherapy is the primary treatment for coronary in-stent restenosis. We hypothesized that differences in dose delivered to target may contribute to treatment failures. We compared dose distribution between arteries that developed recurrent restenosis (treatment failures) and those that remained patent at nine-months (treatment success). A cohort of 207 patients receiving brachytherapy for coronary in-stent restenosis with four radiation delivery devices was followed to identify treatment failures and successes. This cohort was examined to establish which patient and lesion characteristics had an effect on outcome. A nested case-control construct was then used in which treatment failures (n=14) were compared 1:2 …

Assignment Of The Four Disulfides In The N-Terminal Somatomedin B Domain Of Native Vitronectin Isolated From Human Plasma, Nancy A. Horn, Gregory B. Hurst, Anand Mayasundari, Neil A. Whittemore, Engin H. Serpersu, Cynthia B. Peterson

Faculty Publications

The primary sequence of the N-terminal somatomedin B (SMB) domain of native vitronectin contains 44 amino acids, including a framework of four disulfide bonds formed by 8 closely spaced cysteines in sequence patterns similar to those found in the cystine knot family of proteins. The SMB domain of vitronectin was isolated by digesting the protein with endoproteinase Glu-C and purifying the N-terminal 1-55 peptide by reverse-phase high performance liquid chromatography. Through a combination of techniques, including stepwise reduction and alkylation at acidic pH, peptide mapping with matrix-assisted laser desorption ionization mass spectrometry and NMR, the disulfide bonds contained in the …

Sandspur, Vol 111, No 01, August 20, 2004, Rollins College

The Rollins Sandspur

Rollins College student newspaper, written by the students and published at Rollins College. The Sandspur started as a literary journal.

The Hilltop 8-20-2004, Hilltop Staff

The Hilltop: 2000 - 2010

No abstract provided.

Vol.32 N.23 August 19th 2004, Brown Publishing Company

Black Voice News

No abstract provided.

Cuba: Invasion Rumors, Ladb Staff

NotiCen

No abstract provided.

The Effects Of Selenium On Estrogen-Regulated Gene Expression In Lncap Prostate Cancer Cells, Tory L. Parker

Theses and Dissertations

Prostate cancer is the most frequently diagnosed cancer in American men and the second leading cause of cancer deaths. Supplementation with Se has reduced the incidence of prostate cancer and Se status is inversely correlated with prostate cancer risk. One molecular mechanism by which high Se concentrations may affect cancer risk is by catalyzing disulfide bond formation or otherwise complexing with reactive sulfhydryl groups in cellular proteins. The estrogen receptor (ER) contains cysteines in zinc (Zn) fingers that are susceptible to oxidation and internal disulfide formation, which can prevent DNA binding. We examined ER binding to its DNA response element …

The Weekly Challenger, August 19, 2004, The Weekly Challenger, Et Al

Weekly Challenger Newspaper Collection

The August 19, 2004, issue of The Weekly Challenger.

August 2004 General Studies Council Minutes, University Of Nebraska At Kearney General Studies Council

General Education Council

No abstract provided.

Broadening Of A Nonequilibrium Phase Transition By Extended Structural Defects, Thomas Vojta

Physics Faculty Research & Creative Works

We study the effects of quenched extended impurities on nonequilibrium phase transitions in the directed percolation universality class. We show that these impurities have a dramatic effect: they completely destroy the sharp phase transition by smearing. This is caused by rare strongly coupled spatial regions which can undergo the phase transition independently from the bulk system. We use extremal statistics to determine the stationary state as well as the dynamics in the tail of the smeared transition, and we illustrate the results by computer simulations.

Synaptic Transformations Underlying Highly Selective Auditory Representations Of Learned Birdsong, Melissa J. Coleman, Richard Mooney

WM Keck Science Faculty Papers

Stimulus-specific neuronal responses are a striking characteristic of several sensory systems, although the synaptic mechanisms underlying their generation are not well understood. The songbird nucleus HVC (used here as a proper name) contains projection neurons (PNs) that fire temporally sparse bursts of action potentials to playback of the bird's own song (BOS) but are essentially silent when presented with other acoustical stimuli. To understand how such remarkable stimulus specificity emerges, it is necessary to compare the auditory-evoked responsiveness of the afferents of HVC with synaptic responses in identified HVC neurons. We found that inactivating the interfacial nucleus of the nidopallium …