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Using Transcendental Phenomenology To Explore The “Ripple Effect” In A Leadership Mentoring Program, Tammy Moerer-Urdahl, John W. Creswell

Department of Educational Psychology: Faculty Publications

Several approaches exist for organizing and analyzing data in a phenomenological qualitative study. Transcendental phenomenology, based on principles identified by Husserl (1931) and translated into a qualitative method by Moustakas (1994), holds promise as a viable procedure for phenomenological research. However, to best understand the approach to transcendental phenomenology, the procedures need to be illustrated by a qualitative study that employs this approach. This article first discusses the procedures for organizing and analyzing data according to Moustakas (1994). Then it illustrates each step in the data analysis procedure of transcendental phenomenology using a study of reinvestment or the “ripple effect” …

Impacts Of Water Development On Aquatic Macroinvertebrates, Amphibians, And Plants In Wetlands Of A Semi-Arid Landscape, Ned H. Euliss Jr., David M. Mushet

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We compared the macroinvertebrate and amphibian communities of 12 excavated and 12 natural wetlands in western North Dakota, USA, to assess the effects of artificially lengthened hydroperiods on the biotic communities of wetlands in this semi-arid region. Excavated wetlands were much deeper and captured greater volumes of water than natural wetlands. Most excavated wetlands maintained water throughout the study period (May to October 1999), whereas most of the natural wetlands were dry by June. Excavated wetlands were largely unvegetated or contained submergent and deep-marsh plant species. The natural wetlands had two well-defined vegetative zones populated by plant species typical of …

Forest Harvest Can Increase Subsequent Forest Fire Severity, Carter Stone, Andrew T. Hudak, Penelope Morgan

United States Department of Agriculture, Forest Service / University of Nebraska-Lincoln: Faculty Publications

The USDA Forest Service is progressing from a land management strategy oriented around timber extraction towards one oriented around maintaining healthy forested lands. The healthy Forest Initiative promotes the idea of broadscale forest thinning and fuel treatments as an effective means for mitigating hazardous fuel conditions and, by extension, fire risk. Fuels mitigation is proactive while fire suppression is reactive and expensive. Costs associated with suppressing large wildfires, as occur in the western USA with annual regularity, are astronomical and routinely exceed fire suppression budgets. It is not difficult to demonstrate that treating forest fuels is more cost effective than …

Cd46-Mediated Transduction Of A Species D Adenovirus Vaccine Improves Mucosal Vaccine Efficacy, Zenaido T. Camacho, Mallory A. Turner, Michael A. Barry, Eric A. Weaver

Nebraska Center for Virology: Faculty Publications

The high levels of preexisting immunity against Adenovirus type 5 (Ad5) have deemed Ad5 unusable for translation as a human vaccine vector. Low seroprevalent alternative viral vectors may be less impacted by preexisting immunity, but they may also have significantly different phenotypes from that of Ad5. In this study we compare species D Ads (26, 28, and 48) to the species C Ad5. In vitro transduction studies show striking differences between the species C and D viruses. Most notably, Ad26 transduced human dendritic cells much more effectively than Ad5. In vivo imaging studies showed strikingly different transgene expression profiles. The …

Phylogenetic Analyses Of Texas Isolates Indicate An Evolving Subtype Of The Clade B Feline Immunodeficiency Viruses, Eric A. Weaver, Ellen W. Collisson, Margaret Slater, Guan Zhu

Nebraska Center for Virology: Faculty Publications

Rigorous phylogenetic analyses were used to compare the nucleotide sequences of feline immunodeficiency virus strains isolated from Texas and throughout the world. The envelope V3-V4 sequences and capsid gene of the Texas isolates formed a cluster between subtypes B and E. Statistical comparisons with other published sequences confirmed that the Texas group is a unique cluster, possibly a new subtype, arising from subtype B.

Bird Depredation, James F. Glahn, D. Tommy King

United States Department of Agriculture Wildlife Services: Staff Publications

A survey of catfish producers by the United States Department of Agriculture, Centers for Epidemiology and Animal Health (CEAH) in 1996 indicated that the two primary sources of catfish losses in commercial operations were disease (45%) and wildlife (37%) (CEAH 1997a). A variety of avian and mammalian predators are amracred to aquaculture facilities in the United States (Parkhurs: er al. 1992) because ponds and open raceways provide a constant and readily accessible food supply for these animals. However, the mere presence of these predators arcund aquaculture faciliries does not necessarily mean that significant depredation problems are occurring. At catfish farms, …

Pest Control: Rodents, Mark E. Tobin, Michael W. Fall

United States Department of Agriculture Wildlife Services: Staff Publications

Rodents are an important and ubiquitous group of mammals that occur as indigenous or introduced species throughout the world. The populations of a relatively few species that live in close association with humans sometimes cause economic damage or become threats to the health of humans or domestic animals. When rodent control efforts are contemplated, the type of problem and the objectives of these efforts should be carefully defined. Successful management of rodent problems depends upon correct identification of the rodent species involved and on obtaining information on the biology, ecology, and behavior of the species in the ecological setting where …

Coyote And Wolf Habitat Use In Northwestern Montana, Wendy M. Arjo, Daniel H. Peltscher

United States Department of Agriculture Wildlife Services: Staff Publications

Being a habitat generalist is an adaptation suategy that has allowed the coyote to expand its range. As wolves reestablish, or are reintroduced, resident populations of coyotes may change habitat use. We compared habitat use between coyotes and wolves in Glacier National Park after successful recolonization by wolves. Two wolf oacks and nine coyotes were monitored from June 1994 throueh June 1997 to determine habitat use in northwestern Montana. Wolves used habitat types within their home ranges in proportion to availability during the winter, but not the summer when more open areas and burned forests were used. Most coyotes used …

Evaluating Population Management Scenarios: Crunching The Numbers Before Going To The Field, Bradley F. Blackwell, Brian E. Washburn, Michael J. Begier

United States Department of Agriculture Wildlife Services: Staff Publications

Effolts to mitigate wildlife-human conflicts tpically involve management of unacceptably abundant populations. Increasingly, however, reduction of dense or increasing populations of certain wild species evokes both support and contention kom the public. Management decisions involving population reduction, particularly those directed at highly visible species, should therefore be based on quantitative evaluation of potential outcomes prior to implementation. The purpose of th~sp aper is to revisit a call for use of population modeling in management decisions by reviewing basic aspects of population analysis and the use of publicly available long-term data sets in environmental assessments and impact statements. Our objectives are …

Using Geographic Information System (Gis) Software To Predict Blackbird Roosting Locations In North Dakota, Ryan L. Wimberly, Tony A. Slowik, H. Jeffrey Homan, Linda B. Penry

United States Department of Agriculture Wildlife Services: Staff Publications

Cattail stands provide roosting and staging areas for large congregations of blackbirds in North Dakota in late summer and early fall. Since 1991, the U.S. Depamnent of Agriculture, Ammal and Plant Health Inspection Service, Wildlife Services (WS) program has conducted a cattail management program in North Dakota to alleviate blackbird damage to ripening sunflower. To extend the capabilities of the program, a geographical mformation system (GIS) will be incorporated to help WS personnel find blackbird roosts more effectively. We will use the GIs to construct field maps showing the association between areas of moderate to hgh sunilower damage (>5%) …

Evaluation Of A Deer-Activated Bioacoustic Frightening Device For Reducing Deer Damage In Cornfields, Jason M. Gildorf, Scott E. Hygnstrom, Kurt C. Vercauteren, Greg M. Clements, Erin E. Blankenship, Richard M. Engeman

United States Department of Agriculture Wildlife Services: Staff Publications

Deer (Odocoileus spp.) can cause substantial damage to agricultural crops, resulting in economic losses for producers. We developed a deer-activated bio-acoustic frightening device to reduce white-tailed deer (O. virginianus) damage in agricultural fields. The device consisted of an infrared detection system that activated an audio component which broadcast recorded distress and alarm calls of deer. We tested the device against unprotected controls in cornfields during the silking–tasseling stage of growth in July 2001. The device was not effective in reducing damage: track-count indices (F1,4=0.02, P=0.892), corn yield (F1,9=1.27, P=0.289), and estimated damage levels (F1,10=0.87, P=0.374) did not differ between experimental …

Predacides For Canid Predation Management, K. A. Fagerstone, J. J. Johnston, P. J. Savarie

United States Department of Agriculture Wildlife Services: Staff Publications

Throughout the livestock industry in the western United States, control of canid predators was considered to be of considerable importance to the livestock industry, especially to sheep producers, who sufkred high losses from coyotes and wolves. In the 19th century, the demand for predator control was communicated to Congress and the western state assemblies, with the result that predator control was provided in western states by the Fedcral Bureau of Biological Survey in cooperation with state agencies, and by trappers hired by stockmen. Steel traps and poisons were the principal methods used for predator control during the early years of …

Pissing Glass And The Body Crass: Adaptations Of The Scatological In Théophile, Russell J. Ganim

Department of Modern Languages and Literatures: Faculty Publications

Examination of scatological motifs in Théophile de Viau’s (1590-1626) libertine, or ‘cabaret’ poetry is important in terms of how the scatological contributes to the depiction of the Early Modern body in the French lyric.1 This essay does not examine Théophile’s portrait of the body strictly in terms of the ‘Baroque’ or the ‘neo-Classical.’ Rather, it argues that the scatological context in which he situates the body (either his, or those of others), reflects a keen sensibility of the body representative of the transition between these two eras. Théophile reinforces what Bernard Beugnot terms the body’s inherent ‘eloquence’ (17), or what …

Computer Programs For Temperature In Fins And Slab Bodies With The Method Of Green’S Functions, Kevin D. Cole

Department of Mechanical and Materials Engineering: Faculty Publications

Heat conduction in fins and slab bodies, a traditional undergraduate topic, is treated with the Green’s function (GF) method. A variety of boundary conditions and heating conditions are included. Computer programs are described which are designed to improve student learning of the GF method. The programs are available from the author.

The Accidental Plant Pathologist, Anne K. Vidaver

Department of Plant Pathology: Faculty Publications

This article presents the experiences of a woman in academic plant pathology from the 1950s to today. Topics include the social climate for women in science, personal and professional developments and research discoveries, public policy issues in agriculture and biotechnology affecting plant pathology, and projections for the future of plant pathology.

Biography Of James L. Van Etten, Christen Brownlee, James L. Van Etten

Department of Plant Pathology: Faculty Publications

Green algae, in surface layers of almost every lake or stream, are some of the most common aquatic creatures. However, unbeknownst to researchers until recently, viruses that infect algae are almost as widespread. Entire ecosystems of algal hosts and their corresponding viruses lay hidden until the 1980s, when James L. Van Etten, a professor of plant pathology at the University of Nebraska (Lincoln), and his colleague Russ Meints discovered and began to characterize the first member of what is now a rapidly expanding family of algal viruses. Van Etten and his colleagues have continued to study these intriguing viruses, focusing …

A Conceptual Framework For Non-Kin Food Sharing: Timing And Currency Of Benefits, Jeffrey R. Stevens, Ian C. Gilby

Jeffrey Stevens Publications

Many animal species, from arthropods to apes, share food. This paper presents a new framework that categorizes nonkin food sharing according to two axes: (1) the interval between sharing and receiving the benefits of sharing, and (2) the currency units in which benefits accrue to the sharer (especially food versus nonfood). Sharers can obtain immediate benefits from increased foraging efficiency, predation avoidance, mate provisioning, or manipulative mutualism. Reciprocity, trade, status enhancement and group augmentation can delay benefits. When benefits are delayed or when food is exchanged for nonfood benefits, maintaining sharing can become more difficult because animals face discounting and …

Great Plains Ecosystems: Past, Present, And Future, Fred B. Samson, Fritz L. Knopf, Wayne Ostlie

United States Geological Survey: Staff Publications

Little questions exists that the main bodies of North American prairie (i.e., the tall-grass, mixed, and shortgrass) are among the most endangered resources on the continent. The purpose of this paper is to provide a past and present biological base- line by which to understand North American prairies and to provide a platform for future conservation. Events both immediate to the end of the Pleistocene and historic suggest that the present grassland conditions are different from those within which most of the grassland organisms evolved. Our analysis suggests that few grassland landscapes remain adequate in area and distribution to sustain …

Book Review Of Chromatography Of Natural, Treated And Waste Waters Edited By T. R. Crompton, John J. Johnston

United States Department of Agriculture Wildlife Services: Staff Publications

T. R. Crompton has accomplished a significant task, summarizing the literature (up to 1998) for the determination of organic and inorganic compounds in a variety of water substrates (surface/natural water, treated water, and waste water). Overall, the book is relatively easy to read; the manuscript produced by the author contains clear tables, figures, and font. The first chapter presents a brief overview of the techniques presented in the book. The author assumes that the reader has a general understanding of chromatography and the basic components of a chromatographic system. The remainder of the book is organized into chapters by chromatographic …

Safety Of Brucella Abortus Strain Rb51 In Black Bears, Steven C. Olsen, Jack Rhyan, T. Gidlewski, Jesse Goff, W. C. Stoffregen

United States Department of Agriculture Wildlife Services: Staff Publications

In two studies conducted from October 1999 to March 2000 and December 2000 to April 2001, adult black bears (Ursus americanus) were orally inoculated with 1.4–3.1X10¹⁰ colony-forming units (CFU) of Brucella abortus strain RB51 (SRB51, n=12) or 2 ml of 0.15 M NaCl solution (saline, n=11). We did not detect a difference (P>0.05) in antibody titers to SRB51 in serum obtained before vaccination, at 8 wk after vaccination, or at necropsy at 21 or 23 wk after vaccination between SRB51-vaccinated and nonvaccinated bears. The SRB51 vaccine strain was recovered from tissues obtained at …

The State Department Of Education's Role In Creating Safe Schools, Marilyn Grady, Jean Haar, Mary Ann Losh

Department of Educational Administration: Faculty Publications

The authority for public education in the United States does not stem from the Constitution, but rather is a "reserved" power remaining with the states. It originates from the Tenth Amendment, which reserves to the states those powers neither expressly given to the national govennnent nor denied to the state governments. However, most states have not exercised their authority for public education directly until recent decades. Education is a state function that is largely locally administered (Aikin, Linden, Noel, & Ray, 1992). Each state exercises its education function completely or in part through a state department of education that has …

Molecular Mapping And Identification Of Soybean Fatty Acid Modifier Quantitative Trait Loci, D. L. Hyten Jr., Vincent R. Pantalone, Arnold M. Saxton, Michael E. Schmidt, Carl E. Sams

Department of Agronomy and Horticulture: Faculty Publications

Altering FA content in soybean [Glycine max (L.) Merr.] oil for improved functionality is a research goal of many soybean breeders. Several of the genes that alter palmitic, stearic, oleic, linoleic, and linolenic acids are modifier genes with small effects, causing these FA traits to act as quantitative traits. The objective of this study was to identify modifier FA quantitative trait loci (QTL) in soybean. A recombinant inbred line population was created from two prominent ancestors of currently avail-able U.S. cultivars (Essex and Williams) and grown in five environments. One hundred simple se-quence repeat markers spaced throughout the genome …

Reconsidering The Evolution Of Eukaryotic Selenoproteins: A Novel Nonmammalian Family With Scattered Phylogenetic Distribution, Sergi Castellano, Sergey V. Novoselov, Gregory V. Kryukov, Alain Lescure, Enrique Blanco, Alain Krol, Vadim N. Gladyshev, Roderic Guigo

Vadim Gladyshev Publications

While the genome sequence and gene content are available for an increasing number of organisms, eukaryotic selenoproteins remain poorly characterized. The dual role of the UGA codon confounds the identification of novel selenoprotein genes. Here, we describe a comparative genomics approach that relies on the genome-wide prediction of genes with in-frame TGA codons, and the subsequent comparison of predictions from different genomes, wherein conservation in regions flanking the TGA codon suggests selenocysteine coding function. Application of this method to human and fugu genomes identified a novel selenoprotein family, named SelU, in the puffer fish. The selenocysteinecontaining form also occurred in …

Clark’S Nutcrackers (Nucifraga Columbiana) And The Effects Of Goal-Landmark Distance On Overshadowing, Aleida J. Goodyear, Alan Kamil

Papers in Behavior in Biological Sciences

Three groups of Clark’s nutcrackers (Nucifraga columbiana) were trained to find a goal location defined by an array of 4 landmarks that varied in goal–landmark distance. The arrays for each group differed in the distance of the closest landmark and contained goal–landmark distances that were common across groups, allowing for the examination of the effects of both relative and absolute goal–landmark distance on encoding of a landmark array. All 3 groups readily learned the task and were subsequently tested in probe tests with only single landmarks from the array available. Search error in tests with single landmarks was …

A Role For Talin In Presynaptic Function, Jennifer R. Morgan, Gilbert Di Paolo, Hauke Werner, Valentina A. Shchedrina, Marc Pypaert, Vincent A. Pieribone, Pietro De Camilli

Department of Biochemistry: Faculty Publications

Talin, an adaptor between integrin and the actin cytoskeleton at sites of cell adhesion, was recently found to be present at neuronal synapses, where its function remains unknown. Talin interacts with phosphatidylinositol-(4)-phosphate 5-kinase type Iγ, the major phosphatidylinositol-(4,5)-bisphosphate [PI(4,5)P₂]–synthesizing enzyme in brain. To gain insight into the synaptic role of talin, we microinjected into the large lamprey axons reagents that compete the talin–PIP kinase interaction and then examined their effects on synaptic structure. A dramatic decrease of synaptic actin and an impairment of clathrin-mediated synaptic vesicle endocytosis were observed. The endocytic defect included an accumulation of clathrin-coated pits …

Endocytic Function, Glycosaminoglycan Specificity, And Antibody Sensitivity Of The Recombinant Human 190-Kda Hyaluronan Receptor For Endocytosis (Hare), Ed Harris, Janet A. Weigel, Paul H. Weigel

Department of Biochemistry: Faculty Publications

The human hyaluronan receptor for endocytosis (hHARE) mediates the endocytic clearance of hyaluronan (HA) and chondroitin sulfate from lymph fluid and blood. Two hHARE isoforms (190 and 315 kDa) are present in sinusoidal endothelial cells of liver, spleen, and lymph nodes (Zhou, B., McGary, C. T., Weigel, J. A., Saxena, A., and Weigel, P. H. (2003) Glycobiology 13, 339–349). Here we report the specificity and function of the 190-kDa HARE, expressed without the larger isoform, in Flp-In 293 cell lines (190hHARE cells). Like the native protein, recombinant hHARE contains ~25 kDa of N-linked oligosaccharides, binds HA in a ligand blot …

5-Hydroxydecanoate Is Metabolised In Mitochondria And Creates A Rate-Limiting Bottleneck For Β-Oxidation Of Fatty Acids, Peter J. Hanley, Stefan Dröse, Ulrich Brandt, Rachel A. Lareau, Abir L. Banerjee, D. K. Srivastava, Leonard J. Banaszak, Joseph J. Barycki, Paul P. Van Veldhoven, Jürgen Daut

Department of Biochemistry: Faculty Publications

5-Hydroxydecanoate (5-HD) blocks pharmacological and ischaemic preconditioning, and

has been postulated to be a specific inhibitor of mitochondrial ATP-sensitive K+ (KATP)

channels. However, recent work has shown that 5-HD is activated to 5-hydroxydecanoyl-CoA

(5-HD-CoA), which is a substrate for the first step of β-oxidation. We have now

analysed the complete β-oxidation of 5-HD-CoA using specially synthesised (and purified)

substrates and enzymes, as well as isolated rat liver and heart mitochondria, and compared

it with the metabolism of the physiological substrate decanoyl-CoA. At the second step of

β-oxidation, catalysed by enoyl-CoA hydratase, enzyme kinetics were …

Identification Of Methionine-Rich Clusters That Regulate Copper-Stimulated Endocytosis Of The Human Ctr1 Copper Transporter, Yan Guo

Department of Biochemistry: Faculty Publications

Copper uptake and subsequent delivery to copper-dependent

enzymes are essential for many cellular processes.

However, the intracellular levels of this nutrient

must be controlled because of its potential toxicity. The

hCtr1 protein functions in high affinity copper uptake

at the plasma membrane of human cells. Recent studies

have shown that elevated copper stimulates the endocytosis

and degradation of the hCtr1 protein, and this

response is likely an important homeostatic mechanism

that prevents the overaccumulation of copper. The domains

of hCtr1 involved in copper-stimulated endocytosis

and degradation are unknown. In this study we examined

the importance of potential copper-binding

sequences in …

Mobilization Of Intracellular Copper Stores By The Ctr2 Vacuolar Copper Transporter, Erin M. Rees, Jaekwon Lee, Dennis J. Thiele

Department of Biochemistry: Faculty Publications

Copper plays an essential role in processes including signaling to the transcription and protein trafficking machinery, oxidative phosphorylation, iron mobilization, neuropeptide maturation, and normal development. Whereas much is known about intracellular mobilization of ions such as calcium, little information is available on how eukaryotic cells mobilize intracellular copper stores. We describe a mechanism by which the Saccharomyces cerevisiae Ctr2 protein provides bioavailable copper via mobilization of intracellular copper stores. Whereas Ctr2 exhibits structural similarity to the Ctr1 plasma membrane copper importer, microscopic and biochemical fractionation studies localize Ctr2 to the vacuole membrane. We demonstrate that Ctr2 mobilizes vacuolar copper stores …

Interactions Between Small Heat Shock Protein Subunits And Substrate In Small Heat Shock Protein-Substrate Complexes, Kenneth L. Friedrich,, Kim C. Giese, Nicole R. Buan, Elizabeth Vierling

Department of Biochemistry: Faculty Publications

Small heat shock proteins (sHSPs) are dynamic oligomeric proteins that bind unfolding proteins and protect them from irreversible aggregation. This binding results in the formation of sHSP-substrate complexes from which substrate can later be refolded. Interactions between sHSP and substrate in sHSP-substrate complexes and the mechanism by which substrate is transferred to ATP-dependent chaperones for refolding are poorly defined. We have established C-terminal affinity-tagged sHSPs from a eukaryote (pea HSP18.1) and a prokaryote (Synechocystis HSP16.6) as tools to investigate these issues. We demonstrate that sHSP subunit exchange for HSP18.1 and HSP16.6 is temperature-dependent and rapid at the optimal growth temperature …