Digital Commons Network^™

Integrated Allosteric Regulation In The S. Cerevisiae Carbamylphosphate Synthetase – Aspartate Transcarbamylase Multifunctional Protein, Valã©Rie Serre, Bernadette Penverne, Jean-Luc Souciet, Serge Potier, Hedeel Guy, David Evans, Patrick Vicart, Guy Hervã©

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

The S. cerevisiae carbamylphosphate synthetase – aspartate transcarbamylase multifunctional protein catalyses the first two reactions of the pyrimidine pathway. In this organism, these two reactions are feedback inhibited by the end product UTP. In the present work, the mechanisms of these integrated inhibitions were studied.

Results

The results obtained show that the inhibition is competitive in the case of carbamylphosphate synthetase and non-competitive in the case of aspartate transcarbamylase. They also identify the substrate whose binding is altered by this nucleotide and the step of the carbamylphosphate synthetase reaction which is inhibited. Furthermore, the structure of the domains …

Lack Of Chart Reminder Effectiveness On Family Medicine Resident Jnc-Vi And Ncep Iii Guideline Knowledge And Attitudes, Paul S. Echlin, Ross Eg Upshur, Tsveti P. Markova

Wayne State University Associated BioMed Central Scholarship

Abstract

Background

The literature demonstrates that medical residents and practicing physicians have an attitudinal-behavioral discordance concerning their positive attitudes towards clinical practice guidelines (CPG), and the implementation of these guidelines into clinical practice patterns.

Methods

A pilot study was performed to determine if change in a previously identified CPG compliance factor (accessibility) would produce a significant increase in family medicine resident knowledge and attitude toward the guidelines. The primary study intervention involved placing a summary of the Sixth Report of the Joint National Committee on Prevention, Detection, Evaluation, and Treatment of High Blood Pressure (JNC VI) and the National Cholesterol …

Translation Research: From Accurate Diagnosis To Appropriate Treatment, Craig P. Webb, Harvey I. Pass

Wayne State University Associated BioMed Central Scholarship

Abstract

This review article focuses on the various aspects of translational research, where research on human subjects can ultimately enhance the diagnosis and treatment of future patients. While we will use specific examples relating to the asbestos related cancer mesothelioma, it should be stressed that the general approach outlined throughout this review is readily applicable to other diseases with an underlying molecular basis. Through the integration of molecular-based technologies, systematic tissue procurement and medical informatics, we now have the ability to identify clinically applicable "genotype"-"phenotype" associations across cohorts of patients that can rapidly be translated into useful diagnostic and treatment …

Pr 610 C The Servant As Proclaimer, Stacy R. Minger

Syllabi

REQUIRED TEXTBOOKS ______________________________________ Cantalamessa, Raniero. The Mystery of God's Word. Collegeville, MN: The Liturgical Press, 1994. Craddock, Fred B. Preaching. Nashville: Abingdon Press, 1985. Kalas, J. Ellsworth. Preaching from the Soul: Insistent Observations on the Sacred Art. Nashville: Abingdon, 2003. Webb, Joseph M. Preaching without Notes. Nashville: Abingdon, 2001.

Transformational Leadership, Perceived Union Support, And Union Citizenship Behaviors: A Social Exchange And Social Identity Perspective, Nicholas William Twigg Jr.

Doctoral Dissertations

The objective of this dissertation is to empirically assess the relationship between transformational leadership and union citizenship behaviors from a social exchange and social identity perspective. The relationship was studied through a covenantal relationship perspective. Podsakoff, MacKenzie, Paine, and Bachrach (2000) suggested that there were conceptual similarities and differences in the relationships between transformational leadership, perceived organizational support, trust, intrinsic satisfaction, and commitment in an organizational citizenship behavior framework. This dissertation has developed and tested a model that provides a framework to describe the mechanism by which transformational leadership behaviors effect union citizenship behaviors through perceived union support, felt obligation, …

Alumni Journal, Donald C. Doerr, Kate Gaetano, Sara Mortimer, Husna Haq

Syracuse University Magazine

No abstract provided.

Harding Magazine Winter 2004 (Vol. 12, No. 1), Harding University

Harding Magazines

Publication distributed to alumni and friends of the university.

2004 Annual Report, The Jackson Laboratory

Annual Reports

No abstract provided.

The Tiger Claw, 2004, Clarksville High School

All Red River County Yearbooks

This is the yearbook from Clarksville High School for the academic year 2003-04. It contains photographs of students and faculty members, student organizations, athletic teams, and school events. Also included are advertisements for local businesses.

Ec04-464 There's No Excuse For Abuse (Part 1 Of A Four Part Series), Kathy Bosch

University of Nebraska-Lincoln Extension: Historical Materials

In this series of four articles, information will be provided from the Family Violence Prevention Fund, the Nebraska and Kansas Coalitions Against Sexual and Domestic Violence, K-State Research and Extension, Nebraska Cooperative Extension, and many other research-based sources. You will learn more about how you can help a woman who is being abused, how you can offer support to children in abusive situations, how you may approach potential abusers, how to access resources, steps that can be taken in the workplace to help end domestic violence, what to teach children about partner abuse, how to raise community awareness, and how …

Department Of Veterinary And Biomedical Sciences 2004 Annual Report

School of Veterinary and Biomedical Sciences: Information and History

Veterinary and Biomedical Sciences Personnel
VBMS Teaching Program
VBMS Research Program
International Activities, 2004
Veterinary Extension Program. 2004
VBMS Grants and Contracts Program. 2004
Refereed Publications by VBMS Faculty in 2004
Department of Veterinary and Biomedical Sciences. Selected Committees, Editorial and Other Appointments. 2004
Articles Regarding the Department in 2004
Departmental Budget Summaries. 2004
Nebraska Agricultural Statistics. 2003/2004

Primaquine Therapy For Malaria, J. Kevin Baird, Stephen L. Hoffman

United States Public Health Resources

Primaquine is the only available drug for preventing relapse of malaria, and confusion surrounds its use. This review examines the wide range of clinical applications of primaquine described in the medical literature between 1946 and 2004. The risk of relapse of Plasmodium vivax malaria without primaquine therapy ranged from 5% to 80% or more, depending largely upon geographic location. Supervision of therapy profoundly impacts the risk of relapse, and almost all reports of malaria resistant to primaquine are associated with lack of such supervision. We nonetheless suspect that there is widespread resistance to the standard course of primaquine therapy, which …

Review Of "The Contextual Determininants Of Malaria", J. Kevin Baird

United States Public Health Resources

The editors of this book are not malariologists. They are academicians with technical backgrounds in modeling environmental processes. The book they assemble aims to provide “important advances to our understanding of the climate change-malaria linkage.” They assembled a diverse array of authors, most of who are well known in the field of malaria. The authors discuss some controversial and hotly debated issues in the field of malaria control and disagreements abound, but one senses they have disappointed their editors in at least agreeing upon two things: 1) the evidence for a climate change-malaria linkage is, at best, weak, and 2) …

Does Breastfeeding Protect Against Pediatric Overweight? Analysis Of Longitudinal Data From The Centers For Disease Control And Prevention Pediatric Nutrition Surveillance System, Laurence M. Grummer-Strawn, Zuguo Mei

United States Public Health Resources

Objective. To examine whether increasing duration of breastfeeding is associated with a lower risk of overweight in a low-income population of 4-yearolds in the United States.

Methods. Visit data were linked to determine prospectively the duration of breastfeeding (up to 2 years of age) and weight status at 4 years of age. Overweight among 4-year-old children was defined as a body mass index (BMI)-for-age at or above the 95th percentile based on the 2000 Centers for Disease Control and Prevention growth charts. Logistic regression was performed, controlling for gender, race/ethnicity, and birth weight. In a subset of states, links to …

Wif-B Cells As A Model For Alcohol-Induced Hepatocyte Injury, Courtney S. Schaffert, Sandra L. Todero, Benita L. Mcvicker, Pamela L. Tuma, Michael F. Sorrell, Dean J. Tuma

United States Public Health Resources

A potential in vitro model for studying the mechanisms of alcohol-induced hepatocyte injury is the WIF-B cell line. It has many hepatocyte-like features, including a differentiated, polarized phenotype resulting in formation of bile canaliculi. The aim of this study was to examine the effects of ethanol treatment on this cell line. WIF-B cells were cultured up to 96 h in the absence or presence of 25 mM ethanol and subsequently were analyzed for ethanol-induced physiological and morphological changes. Initial studies revealed WIF-B cells exhibited alcohol dehydrogenase (ADH) activity, expressed cytochrome P4502E1 (CYP2E1), and efficiently metabolized ethanol in culture. This cell …

Natural And Technologic Hazardous Material Releases During And After Natural Disasters: A Review, Stacy Young, Lina Balluz, Josephine Malilay

United States Public Health Resources

Natural disasters may be powerful and prominent mechanisms of direct and indirect hazardous material (hazmat) releases. Hazardous materials that are released as the result of a technologic malfunction precipitated by a natural event are referred to as natural-technologic or na-tech events. Na-tech events pose unique environmental and human hazards. Disaster-associated hazardous material releases are of concern, given increases in population density and accelerating industrial development in areas subject to natural disasters. These trends increase the probability of catastrophic future disasters and the potential for mass human exposure to hazardous materials released during disasters. This systematic review summarizes direct and indirect …

Real-Time Fluorescence Pcr Assays For Detection And Characterization Of Heat-Labile I And Heat-Stable I Enterotoxin Genes From Enterotoxigenic Escherichia Coli, Udo Reischl, Mohammad T. Youssef, Hans Wolf, Eija Hyytia-Trees, Nancy A. Strockbine

United States Public Health Resources

To facilitate the diagnosis of enterotoxigenic Escherichia coli (ETEC) infections in humans, we developed and evaluated real-time fluorescence PCR assays for the Roche LightCycler (LC) against the enterotoxin genes commonly present in strains associated with human illness. Separate LC-PCR assays with identical cycling conditions were designed for the type I heat-labile enterotoxin (LT I) and the type I heat-stable enterotoxin (ST I) genes, using the LC hybridization probe format. A duplex assay for ST I with two sets of amplification primers and three hybridization probes was required to detect the major nucleotide sequence variants of ST I, ST Ia and …

West Nile Virus Blood Transfusion-Related Infection Despite Nucleic Acid Testing, Alexandre Macedo De Oliveira, Brady D. Beecham, Susan P. Montgomery, Robert S. Lanciotti, Jeffrey M. Linnen, Cristina Giachetti, Larry A. Pietrelli, Susan L. Stramer, Thomas J. Safranek

United States Public Health Resources

BACKGROUND: A case of West Nile virus (WNV) encephalitis associated with transfusion of blood that did not react when tested for WNV by minipool (MP) nucleic acid testing (NAT) is described. A Nebraska man developed clinical encephalitis 13 days after surgery and transfusion of 26 blood components. Antibody testing confirmed WNV infection. An investigation was initiated to determine the source of this infection.

STUDY DESIGN AND METHODS: The patient’s family members were interviewed to identify risk factors for WNV infection. Residual samples were retested for WNV RNA using transcription-mediated amplification (TMA) assay and two polymerase chain reaction (PCR) assays. …

Chloroquine Resistance In Plasmodium Vivax, J. Kevin Baird

United States Public Health Resources

Emerging resistance to chloroquine (CQ) by Plasmodium vivax threatens the health of the hundreds of millions of people routinely exposed to the risk of infection with this organism. CQ has been the first-line therapy for vivax malaria since 1946 (32, 115). Plasmodium falciparum developed resistance to CQ in the 1950s (110), and today it occurs globally (91). Resistance by P. vivax was unknown until 1989, when Australians repatriated from Papua New Guinea failed routine treatment (94). Subsequent reports affirmed that finding, and CQ-resistant P. vivax (CRPV) was reported from Indonesia (8, 35, 99, 100, 111). Reports from Myanmar (76, 82) …

Farm Fatalities To Youth 1995–2000: A Comparison By Age Groups, Michael Goldcamp, Kitty J. Hendricks, John R. Myers

United States Public Health Resources

Problem: Although a myriad of research illustrates the safety issues related to farm fatalities in youth populations, very little empirical evidence exists that includes work and non-work related farm fatalities to all youths under 20 years of age at the national level.
Methods: This research will use death certificate data for the six years from 1995 to 2000 that were collected by NIOSH from all 50 state vital statistics registries. Demographic data from the 1998 CAIS were used in rate calculations. In addition to providing annual fatality rates and descriptions of the general causes of death, this research will examine …

Agricultural Pesticide Use And Adenocarcinomas Of The Stomach And Oesophagus, W. J. Lee, W. Lijinsky, E. F. Heineman, R. S. Markin, D. D. Weisenburger, M. H. Ward

United States Public Health Resources

Aims: To evaluate the risk of the stomach and oesophageal adenocarcinomas associated with farming and agricultural pesticide use.
Methods: Population based case-control study in eastern Nebraska. Telephone interviews were conducted with men and women diagnosed with adenocarcinoma of the stomach (n = 170) or oesophagus (n = 137) between 1988 and 1993, and controls (n = 502) randomly selected from the same geographical area. Unconditional logistic regression was used to calculate adjusted odds ratios (ORs) for farming and for use of individual and chemical classes of insecticides and herbicides, including pesticides classified as nitrosatable (able to form N-nitroso compounds on …

Neurobehavioral Evaluation For A Community With Chronic Exposure To Hydrogen Sulfide Gas, Steven G. Inserra, Betty L. Phifer, W. Kent Anger, Michael Lewin, Roberta Hilsdon, Mary C. White

United States Public Health Resources

In May 2000, the Agency for Toxic Substances and Disease Registry of the US government conducted a health investigation in response to community concerns regarding ambient and indoor hydrogen sulfide (H₂S), odor, and health symptoms in Dakota City, Nebraska. The objective was to determine whether adult residents in an area with repeated exposure to H₂S showed poorer performance on neurobehavioral tests than unexposed residents. Study participants were required to meet age (≥16 years of age) and length of residency (2 years) eligibility requirements. A battery of computer-assisted standardized neurobehavioral tests was administered in English or Spanish. …

Outbreak Of Pruritic Rashes Associated With Mites — Kansas, 2004

United States Public Health Resources

In late August 2004, the Kansas Department of Health and Environment (KDHE) received reports from the Crawford County Health Department (CCHD) of approximately 300 residents of Pittsburg, Kansas (2000 population: 19,243), seeking care for a pruritic rash of unknown etiology. In early September, three neighboring counties in Kansas and two neighboring states (Missouri and Nebraska) also reported such cases. These events prompted KDHE to request assistance from CDC. Additional cases subsequently were reported in Oklahoma and Texas. This report describes the investigation in Crawford County, Kansas, by public health agencies and entomologists to identify the etiology of the pruritic rash …

Alcohol And Hiv Decrease Proteasome And Immunoproteasome Function In Macrophages: Implications For Impaired Immune Function During Disease, James Haorah, David Heilman, Casey Diekmann, Natalia Osna, Terrence M. Donohue Jr., Anuja Ghorpade, Yuri Persidsky

United States Public Health Resources

Proteasomes (proteinase complexes, PR) and immunoproteasomes (IPR) degrade damaged proteins and affect protein processing required for antigen presentation by mononuclear phagocytes. These critical immune processes are attenuated during progressive HIV-1 infection and are affected by alcohol abuse. To investigate the mechanisms underlying these functional changes, we measured PR and CYP2E1 activities [an ethanol (EtOH) metabolizing enzyme] and reactive oxygen species (ROS) in human monocyte-derived macrophages (MDM) following HIV-1 infection and EtOH treatment. We observed progressive declines of PR activity and PR/IPR contents in HIV-1-infected MDM. PR activity and IPR expression increased after IFN-γ stimulation but reduced after HIV-1 infection. EtOH …

Molecular Characterization Of Noroviruses Detected In Diarrheic Stools Of Michigan And Wisconsin Dairy Calves: Circulation Of Two Distinct Subgroups, Annabel G. Wise, Stephan S. Monroe, Lora E. Hanson, Daniel L. Grooms, Donald Sockett, Roger K. Maes

United States Public Health Resources

Noroviruses have emerged as the leading worldwide cause of acute non-bacterial gastroenteritis in humans. The presence of noroviruses in diarrheic stool samples from calves on Michigan and Wisconsin dairy farms was investigated by RT-PCR. Norovirus-positive samples were found on all eight farms studied in Michigan and on 2 out of 14 farms in Wisconsin. Phylogenetic analyses of partial polymerase and capsid sequences, derived for a subset of these bovine noroviruses, showed that these strains formed a group which is genetically distinct from the human noroviruses, but more closely related to genogroup I than to genogroup II human noroviruses. Examination of …

Transcriptional Regulation Of The Murine Elf3 Gene In Embryonal Carcinoma Cells And Their Differentiated Counterparts: Requirement For A Novel Upstream Regulatory Region, Jingwen Hou, Phillip J. Wilder, Cory T. Bernadt, Brian Boer, Richard M. Neve, Angie Rizzino

United States Public Health Resources

The transcription factor Elf3, which is one of over 25 Ets family members, is expressed in a wide variety of carcinomas and has been shown to promote the transcription of many genes implicated in cancer. To understand how the Elf3 gene is regulated at the transcriptional level, we probed its 5’-flanking region, and we report here the identification of both proximal and distal regions that regulate murine Elf3 promoter activity. In addition to mapping the transcription start site of the Elf3 gene, the work described in this study identifies four cisregulatory elements in the proximal promoter region of the gene. …

N-(Methylamino) Isobutyric Acid Inhibits Proliferation Of Cfsc-2c Hepatic Stellate Cells, T. L. Freeman, G.M. Thiele, L.W. Klassen, B.T. Klassen, M.E. Mailliard

United States Public Health Resources

Activation of hepatic stellate cells (HSCs) involves the induction of ECM protein synthesis and rapid cell proliferation. Thus, agents that interfere with either process could potentially mitigate the development of liver disease by reducing the synthesis of proteins associated with fibrosis or by reducing the number of activated HSC. Previously, we described that the non-metabolizable amino acid analog N-(methylamino)isobutyric acid (MeAIB) reduced hepatic collagen content of rats in a model of CCl₄-induced liver injury, and in vitro studies using CFSC-2G cells indicated that MeAIB directly reduced collagen synthesis. However, the MeAIB-mediated reduction of hepatic collagen, in vivo, …

Anth 250.01: Introduction To Archaeology, John E. Douglas

University of Montana Course Syllabi

No abstract provided.

Anth 265.01: Human Sexuality, Kimber Haddix Mckay, David Garry Kerr

University of Montana Course Syllabi

No abstract provided.

Genre And Persona In Activist Websites, Margaret M.F. Boreman

Electronic Theses and Dissertations

Digital texts present significant challenges to technical communicators in terms of genre and persona. Because of the ubiquity of electronic media, we increasingly embrace digital formats over print-based documents. As a result, technical communicators must now devise the means to cue audiences to the purposes of digital documents in any given discourse community. We can only convey meaning to our Internet audiences through the use of appropriate standards and elements recognizable generic forms and rhetorical cues that we designate as conventions in specific digital discourse. Just as we use file and folder office metaphors for information stored on computers, digital …