Digital Commons Network^™

Hydrogen Peroxide Formation And Actin Filament Reorganization By Cdc42 Are Essential For Ethanol-Induced In Vitro Angiogenesis, Yong Qian, Jia Luo, Stephen S. Leonard, Gabriel K. Harris, Lyndell Millecchia, Daniel C. Flynn, Xianglin Shi

Faculty & Staff Scholarship

No abstract provided.

Single-Nucleotide Polymorphisms In Soybean, Y. L. Zhu, Q. J. Song, D. L. Hyten, C. P. Van Tassel, L. K. Matukumalli, D. R. Grimm, S. M. Hyatt, E. W. Fickus, N. D. Young, P. B. Cregan

Department of Agronomy and Horticulture: Faculty Publications

Single-nucleotide polymorphisms (SNPs) provide an abundant source of DNA polymorphisms in a number of eukaryotic species. Information on the frequency, nature, and distribution of SNPs in plant genomes is limited. Thus, our objectives were (1) to determine SNP frequency in coding and noncoding soybean (Glycine max L. Merr.) DNA sequence amplified from genomic DNA using PCR primers designed to complete genes, cDNAs, and random genomic sequence; (2) to characterize haplotype variation in these sequences; and (3) to provide initial estimates of linkage disequilibrium (LD) in soybean. Approximately 28.7 kbp of coding sequence, 37.9 kbp of noncoding perigenic DNA, and …

Minimum Inhibitory Concentrations Of Two Common Food Phenolic Compounds And Their Effect On The Microbial Ecology Of Swine Feces In Vitro, Jennifer I. Zaffarano

University of Kentucky Master's Theses

Feeding sub-therapeutic levels of antibiotics to livestock has been associated withdevelopment and spread of antibiotic resistant bacteria. The present experiment was conductedto investigate the effect of antibiotic alternatives (caffeic acid, chlorogenic acid, and carbadox)on the microbial ecology of swine feces in vitro.Minimum inhibitory concentrations of caffeic and chlorogenic acids were determined forseveral pathogens using macrobroth and agar dilution techniques. Gram-negative bacteria werenot inhibited. Caffeic acid inhibited four Staphylococcus aureus strains at 200 ppm or less, andtwo Clostridium perfringens strains at 300 ppm. Chlorogenic acid inhibited all S. aureus strainsat 500 ppm, and one C. perfringens strain at 400 ppm.Effects of …

Teachers' And Students' Perceptions Of Seventh- And Eighth-Grade Science Education In A Selected Seventh-Day Adventist Union Conference, Marcel Andre Sargeant

Dissertations

Problem. Science education has long been a great concern in the United States, where less than one-third of the students perform at or above the proficient level. The purpose of this study was to investigate the status of the science program in a selected Union Conference of the Seventh-day Adventist school system. Specifically, this study investigated the perceptions of teachers and students regarding the extent to which the science program meets the criteria of the National Commission on Mathematics and Science Teaching for the 21st century and to what extent these criteria are related to academic performance as indicated by …

The Phenomenon Of Woman-On-Woman Abuse And Its Relationship To Gender Profile And Personal Experiences Of Women, Deborah Johnson Spence

Dissertations

Purpose of the Study. Female-on-female aggression is often inferred, or drawn from studies conducted with children or males. Little or no information is available that reports behaviors perceived as mistreatment or abuse among women. The purposes of this study were to investigate (a) behaviors demonstrated by women that women consider abuse or mistreatment; (b) the extent to which these perceptions of abuse/mistreatment were related to gender profiles; and (c) the extent to which personal experiences as victims or perpetrators of abuse were related to age, race, and education.

Method. This study used the survey research method in which questionnaires …

A Comparative Study Of Giving And Volunteering Patterns Among Adults With Seventh-Day Adventist And Other Christian Religious Identities, Karen E. Stockton-Chilson

Dissertations

Problem. In an environment that challenges the non-profit sector, including church-based organizations, to do more with less, it is important to understand the mechanisms that build and sustain two key resources-individual giving and volunteering. Research indicates that there is a great deal of variation in the extent to which individuals provide help. An integrative theoretical model is utilized to examine and compare the giving and volunteering behaviors, socialization, attitudes, reasons, and motivations of adults with Seventh-day Adventist and other Christian religious identities, with secondary consideration given to the effect of potential ethnic variations.

Method. The sample (N = 1359) …

Development Of A Database And System For Analyzing The Actual And Potential Impacts On The Environment Of Existing And Planned Indot Sites, Lynn A. Corson

JTRP Technical Reports

The objectives of this research were: 1. to identify the source, character, currency and format of environmental, ecological, geological and other data for each existing facility site and organize it in a “hard copy” and/or computerized framework to aid the prioritization and planning process for facility upgrades to protect the environment, for regulatory compliance, and for potential replacement or relocation of existing sites; 2. to identify and assess the criteria currently employed by INDOT to select locations for new and/or relocated facilities, including the ASTM Phase I and II Environmental Site Assessment criteria, and to recommend additional criteria including those …

31_1_Inside_Front_Cover.Pdf, Marine Ornithology

Marine Ornithology

No abstract provided.

31_1_Gen_Info.Pdf, Marine Ornithology

Marine Ornithology

No abstract provided.

31_2_Inside_Front_Cover.Pdf, Marine Ornithology

Marine Ornithology

No abstract provided.

Comparison Between The Ultraviolet Emission From Pulsed Microhollow Cathode Discharges In Xenon And Argon, Isfried Petzenhauser, Leopold D. Biborosch, Uwe Ernst, Klaus Frank, Karl H. Schoenbach

Bioelectrics Publications

We measured the dynamic I–V characteristics and vacuum ultraviolet (VUV) emission lines of the second continuum in xenon (170 nm) and argon (130.5 nm) from pulsed microhollow cathode discharges (MHCD). For pulse lengths between 1 and 100 μs the dynamic I–V characteristics are similar in both inert gases. Only the time variation of the VUV emission line at 170 nm for xenon can be related to the dimer excited states. In argon the energy transfer between the Ar*₂ dimers and the oxygen impurity atoms is responsible for a qualitatively different time behavior of the resonance line at 130.5 nm. …

Electrofusion Of Saccharomyces Cerevisiae Auxotrophic Mutants Of Identical Mating Type Using A Laboratory-Made System, Gökhan Coral, Ömer Çolak, M. Ni̇sa Ünaldi, Burhan Arikan

Turkish Journal of Biology

Yeast protoplasts from haploid auxotroph strains DC6 (MAT\alpha) and FY73 (MAT\alpha) were exposed to an inhomogeneous alternating field (AC 200 V/cm). Due to the dielectrophoretic aggregation two or more cells with close membrane contact were formed between the two electrodes. The effects of different DC pulses were investigated to determine critical fusogenic pulse strength. In the results, the maximum fusant cells were obtained only when 8 kV field strengths were applied. These findings showed that the optimum fusogenic pulse strength for yeast protoplasts is 8 kV/cm under our experimental conditions. After fusion, the DNA contents of both hybrid and individual …

Extremely Halophilic Bacterial Communities In Şereflikoçhisar Salt Lake In Turkey, Meral Bi̇rbi̇r, Cenk Sesal

Turkish Journal of Biology

Şereflikoçhisar Lake is the largest salt lake in central Turkey. This lake is a major source of solar salt for food, hide and other industries locally. Due to the economic importance of salt obtained from this lake, a microbial survey has been conducted. Six salt and three brine samples were obtained from the lake for physico-chemical and microbiological analyses. Physico-chemical analysis showed that the salts and brines contain sufficient ions and hardness to support extremely halophilic bacteria. The salt collected from the lake contained 10^4-10^6 colony-forming units of extremely halophilic bacteria per gram and brine taken from the lake contained …

Biosorption Of Copper Ions By Caustic Treated Waste Baker's Yeast Biomass, Yekta Göksungur, Si̇bel Üren, Ulgar Güvenç

Turkish Journal of Biology

Waste baker's yeast (Saccharomyces cerevisiae) was used as a biosorbent for Cu^{+2} biosorption. The yeast cells were treated with caustic soda, ethanol and heat to increase their biosorption capacity. Among the treatment methods used, the highest copper uptake (21.1 mg g^{-1}) was obtained with the caustic treatment of baker's yeast. The effect of initial copper concentration and pH on biosorption for caustic treated yeast was studied. The highest Cu^{+2} uptake (120.7 mg g^{-1}) was obtained at pH 4.0, for 198.2 mg I^{-1} initial copper ion concentration. The Langmuir model and Freundlich equation were applied to the experimental data and the …

Protein And Dna In Systematic Biology, Selma Gülbi̇tti̇ Onarici, Si̇bel Sümer

Turkish Journal of Biology

Since the 1960s, biological macromolecules have occupied an increasingly important role in evolutionary and systematic studies. These biological macromolecules, used as sources of molecular characteristics, are proteins and various types of DNA such as a nuclear genome, nuclear ribosomal DNA, mitochondrial genome, and chloroplast genome. Electrophoresis and immunological techniques are used for generating systematic data from proteins. Generally, electrophoretic methods focus on two general forms of protein data, isozymes and allozymes, while the basis of immunological studies is antigen-antibody reactions. Basic methodologies for using DNA for systematic purposes include DNA-DNA hybridizations, restriction enzyme analysis, sequencing, cloning, PCR and PCR-based fingerprinting …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Investigation Of The Antimicrobial Activity Of Some Streptomyces Isolates, Nuretti̇n Şahi̇n, Aysel Uğur

Turkish Journal of Biology

Seventy-four different Streptomyces isolates were obtained from the soils of Muğla province. Antimicrobial activity was determined in 45.9% of the isolates. Fifteen isolates showed strong activity against coagulase-negative Staphylococcus (CoNS). These isolates were extensively studied for their in vitro antimicrobial activity against Gram-positive and Gram-negative bacteria and yeasts. The results indicated that five isolates were highly active against CoNS and yeast with an inhibition zone at ? 20 mm. Three of these isolates were identified as Streptomyces antibioticus (MU106, MU107) and S. rimosus (MU114). The UV spectra of the culture extracts for the active isolates showed absorbance peaks ranging between …

Quorum Sensing In Gram-Negative Bacteria, Gülgün Boşgelmez Tinaz

Turkish Journal of Biology

It has become increasingly and widely recognised that bacteria do not live as isolated entities but instead exist as communities that exploit elaborate systems of intercellular communication to facilitate their adaptation to changing environmental conditions. A well-characterised example of such intercellular communication is quorum sensing. Quorum sensing depends on the production of diffusible signal molecules termed autoinducers or pheromones, which enable a bacterium to monitor its own cell population density. A variety of physiological processes in a range of bacterial species is regulated by quorum sensing. Examples include bioluminescence, antibiotic biosynthesis, swarming, biofilm differentiation, conjugation and the production of virulence …

In Vivo And In Vitro Micrografting Of Pistachio, Pistacia Vera L. Cv. "Siirt", Ahmet Onay, Vedat Pi̇ri̇nç, Fi̇li̇z Adiyaman, Çi̇ğdem Işikalan, Engi̇n Ti̇lkat, Davut Başaran

Turkish Journal of Biology

In this study, the success of in vivo and in vitro micrografts of pistachio (Pistacia vera L. cv. "Siirt") materials are presented. The only variable tested was age (1, 5, 10, and 30-year-old trees). Ten- to 12-day-old axenic seedlings germinated in vitro or seedlings (3 to 5 months-old) grown in pots in vivo were used as rootstocks. Shoot tips collected from the four age classes of mature trees of pistachio were the source of scions. Firm contact between the scion and rootstock was assured through the use of parafilm tape at the graft junction for in vivo micrografts. The in …

Adventitious Shoot Organogenesis And Plant Regeneration From Cotyledons Of Diploid Diyarbakır Watermelon (Citrullus Lanatus Cv. "Sürme"), Vedat Pi̇ri̇nç, Ahmet Onay, Fi̇li̇z Adiyaman, Çi̇ğdem Işikalan, Engi̇n Ti̇lkat, Davut Başaran

Turkish Journal of Biology

Adventitious shoots were obtained from the diploid Diyarbakır watermelon cultivar "Sürme" by culturing cotyledons on shoot regeneration medium for 3 weeks. The effects of two cytokinins, benzyl amino purine (BA) and kinetine (Kin), on shoot organogenesis were examined. The number of shoots per explant were approximately more than 50% higher when 0.5 mg/l BA was used compared to the most effective kinetin concentration (1 mg/l). Plants were obtained by transferring 1 to 2 cm shoots to MS medium supplemented with 1-4 mg/l naphthalene acetic acid. Most of the regenerated plants (more than 50%) were successfully transplanted to the field. This …

Investigation Of The Microfungal Flora Of The Bird Paradise National Park In Bandırma, Balıkesir (Turkey), Ayşe Di̇lek Azaz

Turkish Journal of Biology

Fifteen soil samples taken from the Bird Paradise National Park in Bandırma were investigated using the soil dilution plate and soil washing methods. Thirty-three species and four different sterile microfungal taxa were identified. Twenty-eight of these species belonged to the Hyphomycetes and 5 to the Mucorales. The genus with the highest species diversity found in the study area was Penicillium, represented by 16 species. Some chemical properties of the soil samples were also established. Quantitative analysis based on the soil dilution plate method revealed a statistically significant difference between soil samples subjected to the rising water level of the lake …

Cover And Contents

Turkish Journal of Biology

No abstract provided.

Cover And Contents

Turkish Journal of Biology

No abstract provided.

The Development Of A Modified Method For Isolating Plasmids From Exopolysaccharide Producing Lactobacillus Species Using Conventional Plasmid Isolation Methods, Osman Çataloluk

Turkish Journal of Biology

Lactic acid bacteria (LAB) are a key element in the dairy industry. The gene products encoded by the plasmids of LAB carry out fermentation. To date, many successful plasmid isolation studies have been conducted with Escherichia coli, Staphilococcus, Streptococcus, Salmonella, Listeria and many strains of LAB. However, since LAB comprise a conglomerate of seven different genera, each with its own physiological and morphological characteristics, and produce a large amount of exopolysaccharide coat, it is extremely difficult to obtain plasmids by conventional methods. A field isolate of lactobacillus known as Lactobacillus salivarius M7 was utilized to obtain plasmids by all known …

Characterization Of Salivaricin B, A Protein Expressed By Lactobacillus Salivarius M7, Osman Çataloluk, Candan G. Gürakan

Turkish Journal of Biology

Salivaricin B (SalB) is a protein encoded by Lactobacillus salivarius M7. This protein is insensitive to proteolytic enzymes, such as pepsin, trypsin, and chymotrypsin, but is partially sensitive to proteinase K. SalB is also stable and maintains its activity over a range of pHs, from 3 to 9. SalB was found to be effective against many closely related lactic acid bacteria such as, L. fermentum, L. plantarum, L. acidophilus and distantly related gram-positive bacteria, including Listeria monocytogenes, Streptococcus faecalis, Staphylococcus aureus and Staph. epidermidis. The mode of action of SalB was obviously bactericidal, and death occurs without bacterial lysis. The …

Modification By Compositional Antimutagens Of The Key Stages Of A Mutation Process, Urkhan Alekperov, Ali Aliev, Afet Rustamova, Saida Jafarova

Turkish Journal of Biology

In experiments with Escherichia coli B/r WP2(trpE-), mice marrow cells and human lymphocytes the antimutagenic activity of an extract from Zizyphus mill was performed. The extract was tested separately and in mixture with Triticum aestivum. In experiments with different treatment schemes it has been established that the extract from wheat sprouts added to an antimutagenic preparation from Zizyphus mill intensifies the genoprotective properties of the Zizyphus mill extract.

The Effects Of Dietary Fatty Acids On The Fatty Acid Composition In The Phospholipid Fraction Of Gambusia Affinis, Eli̇f İpek Cengi̇z, Erhan Ünlü, Mehmet Başhan

Turkish Journal of Biology

The present study represents an investigation into the effect of dietary fatty acids on the fatty acid composition of the phospholipid fraction of Gambusia affinis, a small freshwater fish living in a source located on the campus of Dicle University. The fatty acid composition of the phospholipid fraction was analysed by gas chromatography for fish collected from the freshwater source, which were naturally fed, and also fish fed an artificial diet with a w6/w3 ratio of 5.5:1 for 30 days. The level of w3 in the fish fed artificially for 30 days was higher than in those collected from the …

Antimicrobial Activities Of Various Medicinal And Commercial Plant Extracts, D. Ayfer Ateş, Özlem Turgay

Turkish Journal of Biology

The antibacterial activities of the alcohol, ethyl acetate, acetone and chloroform extracts of 5 plant species were studied. The extracts of Pimpinella anisum (L.) (anise, aniseed) (seed), Coriandrum sativum (L.) (coriander, cilantro) (seed), Glycyrrhiza glabra (L.) (liquorice) (root), Cinnamomum cassia Blume (cassia bark, Chinese cinnamon) (bark), and Juniperus oxycedrus (L.) (juniper) (seed) were tested in vitro against 13 bacterial species and strains by the agar diffusion method. Bacillus brevis FMC 3, Bacillus cereus EU, Bacillus megaterium DSM 32, Bacillus subtilis IMG 22, Bacillus subtilis var. niger ATCC 10, Enterococcus faecalis, Klebsiella pneumoniae FMC 5, Listeria monocytogenes SCOTT A, Micrococcus luteus …

The Effect Of Tannery Effluent On The Colonization Rate Of Plankters: A Microcosm Study, Yalavarthi Naga Koteswari, Ravichandran Ramanibai

Turkish Journal of Biology

The present study describes a multispecies toxicity test that uses community level responses of plankton samples from a natural lake using polyurythane foam (PF) as artificial substrate. These substrates were suspended and left for a period of 14 days. Colonized substrates were then placed in a series of microcosms dosed with a gradient of tannery effluent from 1 to 15% concentration. The species composition and abundance of the planktonic communities in PF substrate with the exposure of 4 different concentration of tannery effluent (1%, 3%, 5% and 15%) were assessed on days 1, 3, 7, 14, 21 and 28. Species …