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Method To Detect The Presence Of A Microorganism Or Agent In An Animal, David R. Smith, Laura L. Hungerford, Rodney A. Moxley, Susanne Hinkley, Terry Klopfenstein, Jeff Gray

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention provides a method to detect the presence of a microorganism or agent in an animal. The method encompasses placement of devices at various locations where the animal resides so as to induce the animal to initiate contact with the device. As a result of this contact, the animal deposits various microorganisms and agents on the device. The device is then tested for the presence of the particular microorganism or agent of interest.

Duration Of Infection And Proportion Of Pigs Persistently Infected With Porcine Reproductive And Respiratory Syndrome Virus, Robert W. Wills, Alan R. Doster, Judith A. Galeota, Jung-Hyang Sur, Fernando A. Osorio

Nebraska Center for Virology: Faculty Publications

Understanding the dynamics of porcine reproductive and respiratory syndrome virus (PRRSV) persistence in individual pigs is essential to the development of successful control programs. The objectives of this study were to investigate the proportion of inoculated pigs that become persistently infected with PRRSV and the duration of their infection. Additionally, different diagnostic techniques that detect persistent infections were compared. Twenty-eight 35-day-old pigs were inoculated with PRRSV. Serum and tonsil biopsy samples were collected on days 0, 7, 14, and 28 and then approximately monthly thereafter until day 251 post-inoculation (p.i.). Tonsil, lymph node, and lung samples were collected following euthanasia …

Roles Of Mycobacterium Smegmatis D-Alanine:D-Alanine Ligase And D-Alanine Racemase In The Mechanisms Of Action Of And Resistance To The Peptidoglycan Inhibitor D-Cycloserine, Zhengyu Feng, Raul G. Barletta

School of Veterinary and Biomedical Sciences: Faculty Publications

D-Cycloserine (DCS) targets the peptidoglycan biosynthetic enzymes D-alanine racemase (Alr) and Dalanine: D-alanine ligase (Ddl). Previously, we demonstrated that the overproduction of Alr in Mycobacterium smegmatis determines a DCS resistance phenotype. In this study, we investigated the roles of both Alr and Ddl in the mechanisms of action of and resistance to DCS in M. smegmatis. We found that the overexpression of either the M. smegmatis or the Mycobacterium tuberculosis ddl gene in M. smegmatis confers resistance to DCS, but at lower levels than the overexpression of the alr gene. Furthermore, a strain overexpressing both the alr and ddl …

Light Storage With Light Of Arbitrary Polarization, Hong Gao, Mark Rosenberry, Herman Batelaan

Department of Physics and Astronomy: Faculty Publications

We have demonstrated the phase coherence of stored light in Rb vapor with a completely optical technique. Combining this technique with polarization measurements provides strong evidence that arbitrary polarizations can be stored. The fidelity obtained exceeds 95% for all polarizations. We view the capability to store polarizations as a first step towards building a quantum memory in such a system.

Public Knowledge And Perceptions Of Black-Tailed Prairie Dogs, Berton Lee Lamb, Kurt Cline

United States Geological Survey: Staff Publications

Black-tailed prairie dogs (Cynomys ludovicianus) historically occupied an 11-state region of the United States. We surveyed 1,900 residents (response rate 56%) of this region to understand citizen knowledge and perceptions about prairie dogs and their management. Those who have direct experience— e.g., those who live very close to prairie dog colonies or know the location of the nearest colony—have higher levels of knowledge. A significantly higher level of knowledge was documented among those who were politically active when compared with the general public. Those who found environmental issues difficult to understand were associated with lower knowledge. People with …

Influence Of Nitrogen And Duration Of Weed Interference On Corn Growth And Development, Sean P. Evans, Stevan Z. Knezevic, John L. Lindquist, Charles A. Shapiro

Department of Agronomy and Horticulture: Faculty Publications

An improved understanding of the effects of nitrogen (N) on crop–weed interactions is needed for the development of integrated weed management systems where responsible use of N fertilizers is considered. Field experiments conducted in 1999 and 2000 at two locations in eastern Nebraska quantify the effects of N and increasing duration of weed interference on corn growth and development. A naturally occurring population of weeds was allowed to compete with the corn crop for increasing lengths of time and at three rates of N application (0, 60, and 120 kg N ha^-1). Weed interference and withholding applied N …

A Phylogenetic Study Of Cytochrome B561 Proteins, Wim Verelst, Han H. Asard

Department of Biochemistry: Faculty Publications

Background: As an antioxidant and cofactor to numerous metabolic enzymes, ascorbate has an essential role in plants and animals. Cytochromes b561 constitute a class of intrinsic membrane proteins involved in ascorbate regeneration. Despite their importance in ascorbate metabolism, no evolutionary analysis has been presented so far on this newly described protein family. Results: Cytochromes b561 have been identified in a large number of phylogenetically distant species, but are absent in fungi and prokaryotes. Most species contain three or four cytochrome b561 paralogous proteins, and the encoding genes usually have four or five exons. At the protein level, sequence similarities are …

Functional Copper At The Acetyl-Coa Synthase Active Site, Javier Seravalli, Welwel Gu, Annie Tam, Erick Strauss, Stephen P. Cramer, Stephen W. Ragsdale

Department of Biochemistry: Faculty Publications

The bifunctional CO dehydrogenase/acetyl-CoA synthase (CODH/ACS) plays a central role in the Wood–Ljungdahl pathway of autotrophic CO₂ fixation. A recent structure of the Moorella thermoacetica enzyme revealed that the ACS active site contains a [4Fe-4S] cluster bridged to a binuclear Cu-Ni site. Here, biochemical and x-ray absorption spectroscopic (XAS) evidence is presented that the copper ion at the M. thermoacetica ACS active site is essential. Depletion of copper correlates with reduction in ACS activity and in intensity of the ‘‘NiFeC’’ EPR signal without affecting either the activity or the EPR spectroscopic properties associated with CODH. In contrast, Zn content …

Transmembrane Movement Of Exogenous Long-Chain Fatty Acids: Proteins, Enzymes, And Vectorial Esterification, Paul N. Black, Concetta C. Dirusso

Department of Biochemistry: Faculty Publications

INTRODUCTION......................................................................................................................................................454

FATTY ACIDS AND BIOLOGICAL MEMBRANES.............................................................................................455

Fatty Acid Transport Defined ...............................................................................................................................456

MODEL SYSTEMS TO INVESTIGATE FATTY ACID TRANSPORT ..............................................................456

Genetic Foundations of Fatty Acid Transport....................................................................................................456

PROTEINS IMPLICATED IN FATTY ACID TRANSPORT ...............................................................................456

Fatty Acid Translocase..........................................................................................................................................457

Fatty Acid Binding Protein—Membrane Bound ................................................................................................457

Fatty Acid Transport Protein...............................................................................................................................458

The Long-Chain Fatty Acid Transport Protein FadL .......................................................................................458

Fatty Acyl-CoA Synthetase ...................................................................................................................................459

VECTORIAL ACYLATION: ONE MECHANISM OPERATIONAL IN FATTY ACID TRANSPORT...........460

THE BACTERIAL PARADIGM ...............................................................................................................................460

Energetics of Fatty Acid Transport in Gram-Negative Bacteria......................................................................460

The Fatty Acid Transporter FadL........................................................................................................................461

The Fatty Acyl-CoA Synthetase FadD..................................................................................................................461

Structural Considerations of FACS .....................................................................................................................462

FATTY ACID TRANSPORT AND ACTIVATION IN YEAST: …

Single-Cell Protein Profiling Of Wastewater Enterobacterial Communities Predicts Disinfection Efficiency, Gomathinayagam Ponniah, Han Chen, Ronda Michielutti, Nancy Salonen, Paul H. Blum

School of Biological Sciences: Faculty Publications

The efficiency of enterobacterial disinfection is dependent largely on enterobacterial community physiology. However, the relationship between enterobacterial community physiology and wastewater processing is unclear. The purpose of this study was to investigate this relationship. The influence of wastewater treatment processes on enterobacterial community physiology was examined at the single-cell level by using cultureindependent methods. Intracellular concentrations of two conserved proteins, the growth-related protein Fis and the stationary-phase protein Dps, were analyzed by epifluoresence microscopy of uncultivated cells by using enterobacterial group-specific polyclonal fluorochrome-coupled antibodies. Enterobacterial single-cell community protein profiles were distinct for different types of biological treatment. The differences were …

Large-Scale Cultivation Of Acidophilic Hyperthermophiles For Recovery Of Secreted Proteins, Penny Worthington, Paul H. Blum, Francisco Perez-Pomares, Tom Elthon

School of Biological Sciences: Faculty Publications

An electric water heater was modified for large-scale cultivation of aerobic acidophilic hyperthermophiles to enable recovery of secreted proteins. Critical changes included thermostat replacement, redesign of the temperature control circuit, and removal of the cathodic anticorrosion system. These alterations provided accurate temperature and pH control. The bioreactor was used to cultivate selected strains of the archaeon Sulfolobus solfataricus and other species within this genus. Reformulation of a basal salts medium facilitated preparation of large culture volumes and eliminated sterilization-induced precipitation of medium components. Substrate induction of synthesis of the S. solfataricus-secreted alpha-amylase during growth in a defined medium …

Putative Subunits Of The Maize Origin Of Replication Recognition Complex Zmorc1±Zmorc5, Xiaohong Witmer, Raul Alvarez-Venegas, Phillip San-Miguel, Olga Danilevskaya, Zoya Avramova

School of Biological Sciences: Faculty Publications

The finding in animal species of complexes homologous

to the products of six Saccharomyces cerevisiae

genes, origin of replication recognition

complex (ORC), has suggested that ORC-related

mechanisms have been conserved in all eukaryotes.

In plants, however, the only cloned putative homologs

of ORC subunits are the Arabidopsis ORC2 and

the rice ORC1. Homologs of other subunits of plant

origin have not been cloned and characterized. A

striking observation was the absence from the

Arabidopsis genome of an obvious candidate genehomolog

of ORC4. This fact raised compelling

questions of whether plants, in general, and

Arabidopsis, in particular, may have lost the …

2003-2004 Admissions Catalog, New York Law School

NYLS Bulletins and Catalogs

No abstract provided.

Eight Gram-Negative Bacteria Are 10,000 Times More Sensitive To Cationic Detergents Than To Anionic Detergents, Soumitra Rajagopal, Nicole Eis, Kenneth Nickerson

Kenneth Nickerson Papers

In liquid culture, eight typical Gram-negative bacteria were ca. 10,000-fold more sensitive to cationic detergents than to the anionic detergent sodium dodecyl sulfate. Cetyltrimethylammonium bromide (CTAB) was inhibitory at concentrations ranging from 0.0006% to 0.01%. Four pseudomonads able to form biofilms were ca. 1000-fold more resistant to CTAB on Luria–Bertani agar plates than they were in liquid culture. A lasI mutant of Pseudomonas aeruginosa was only able to tolerate 0.1% CTAB on Luria–Bertani agar plates but could tolerate 5% CTAB when supplemented with homoserine lactone containing culture supernatants.

En culture liquide, huit bactéries Gram négatif typiques étaient ca. 10,000 fois …

Hearing Screening Of Neonates At Risk, Alwan M. Maisoun, Siraj M. Zakzouk

Saudi Medical Journal

OBJECTIVE: The aim of this study is to screen infants admitted to the Neonatal Intensive Care Unit (NICU) for hearing loss. METHODS: One hundred and thirty newborns admitted to NICU were screened for hearing loss using otocaustic emission (OAE). Tympanometry was performed using Grason Stadler Incorporation (GSI) 37 and auditory brain response (ABR). RESULTS: Ninety-six of the infants had known risk factors such as prematurely, low birth weight, hyperbilirubinemia, asphyxia and congenital abnormalities. Out of 130 infants 80 passed the OAE test, 50 had to be rescreened, and 19 had ABR. Only 13 were found with moderate to severe hearing …

Otolaryngologic Manifestation Of Gastroesophageal Reflux In Children, Ahmed Y. Al-Ammar

Saudi Medical Journal

OBJECTIVE: This study was performed to assess the role of gastroesophageal reflux (GER) in varieties of otolaryngologic dysfunction. METHODS: This study was carried out over a one year period, between January 1995 and January 1996, at Sainte-Justine Hospital, Montreal, Canada. Thirty-seven charts were examined for different variables including, symptoms, signs, dysfunction, investigations, treatment and outcome. RESULTS: In children with otolaryngologic manifestation of GER, apnea and regurgitation were common presenting symptoms. Gastroesophageal reflux was also found to be more commonly associated with ear infection, adenoid hypertrophy, bronchial asthma and recurrent pneumonia. CONCLUSION: Gastroesophageal reflux has complicated manifestations in otolaryngology that are …

Effects Of Crustal Stresses On Fluid Transport In Fractured Rock: Case Studies From Northeastern And Southwestern Usa, Roger H. Morin, William Z. Savage

United States Geological Survey: Staff Publications

The link between stress and hydrologic properties was examined at two sites that are distinguished by different rock types and different stress states. This investigation is based upon the analysis and interpretation of geophysical logs obtained in water wells at the two locations. At the northeast site (Newark Basin), the hydrologic characteristics of sedimentary rocks are dependent upon the relationship to the current regional stress field of two primary types of orthogonal features that serve as preferential pathways for fluid flow. Subhorizontal bedding-plane partings are highly transmissive near the surface and delineate transversely isotropic fluid flow at shallow depths. With …

Taenia Pencei N. Sp. From The Ringtail, Bassariscus Astutus (Carnivora: Procyonidae), In Texas, U.S.A., Robert L. Rausch

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Taenia pencei n. sp. is described from the ringtail, Bassariscus astutus (Carnivora: Procyonidae), in western Texas, U.S.A., and its metacestode is characterized on the basis of specimens from Peromyscus cf. maniculatus in southern Oregon. Taenia pencei n. sp. apparently occurs exclusively in the ringtail, and it is the first cestode of the genus Taenia known from a member of the family Procyonidae. It is morphologically most similar to species of Taenia from mammals of the family Mustelidae in subclade two of the recently constructed phylogeny of that genus. Taenia pencei is distinguished from the nearctic Taenia taxidiensis in the badger …

Loathing, Jessica Rose Monsour

Theses

This thesis project explores an artist's struggle with self in relationship to environment and society. Specifically, the artist examines loathing, as a shadow that is representative of one's unacceptable attributes that are labeled by society's expectations, through the combination of three processes: photography, sculpture and installations.

Insuring Against Terrorism -- And Crime, Saul Levmore, Kyle D. Logue

Articles

The attacks of September 11th produced staggering losses of life and property. They also brought forth substantial private-insurance payouts, as well as federal relief for the City of New York and for the families of individuals who perished on that day. The losses suffered during and after the attacks, and the structure of the relief effort, have raised questions about the availability of insurance against terrorism, the role of government in providing for, subsidizing, or ensuring the presence of such insurance, as well as the interaction between relief and the incentives for taking precautions against similar losses in the future. …

The Genie And The Bottle: Collateral Sources Under The September 11th Victim Compensation Fund, Kenneth S. Abraham, Kyle D. Logue

Articles

The September 11th Victim Compensation Fund of 2001 (the Fund) was part of legislation enacted just eleven days after the terrorist attacks of September 11th in the wake of extraordinary national loss. It is possible, therefore, that the Fund will always be considered an urgent and unique response to the unprecedented events of September 11th. On that view, the character of the Fund will have little longterm policy significance. It is equally possible, however, that the enactment of the Fund will prove to be a seminal moment in the history of tort and compensation law. The Fund adopts a new …

Proposed Patent Local Rules For Adoption By Texas' Federal District Courts, Alfonso Garcia Chan

Computer Law Review and Technology Journal

This paper proposes a set of local rules to govern patent cases litigated in Texas' U.S. District Courts. The Proposed Patent Local Rules combine the patent rules implemented by U.S. District Judge T. John Ward of the Eastern District of Texas and the most-recent version of the Patent Local Rules of the U.S. District Court for the Northern District of California. The Proposed Patent Local Rules also incorporate comments from the Litigation Committee of the State Bar of Texas Intellectual Property Law Section. The Rules build upon the successes of existing rules in these other courts and can provide trial …

Should Patent Protection Be Considered For Computer Software-Related Innovations?, Elosie Gratton

Computer Law Review and Technology Journal

The twentieth century witnessed the development of more significant scientific and technological advances than any other period in history. In today's society computer-related technologies, including software, are at the forefront of change. But at the beginning of the computer age the computer industry overlooked software in favor of more-valuable computer hardware.

The question of software patentability arose during the 1960's and the early 1970's, when the prevailing view of computer suppliers was that software should not be protected by copyright or patent. The industry believed that a system allowing the free exchange and availability of computer programs might stimulate the …

Information Systems Security And Privacy Issues In The Armed Forces, John C. Dolak, Anna E. Dolak

Computer Law Review and Technology Journal

Members of our Armed Forces serve in foreign countries and throughout the United States. Service members and civilian employees working for the military are permitted limited personal use of military computer systems. This limited usage gives, for example, a sailor on a ship a way to communicate with his parents thousands of miles away. It also means that the military must balance the privacy expectations of users against the need to maintain secure computer systems. The military must also ensure that its systems are not used in an illegal fashion or for illegal purposes. Finally, the military must protect its …

Spinning And Underpricing: A Legal And Economic Analysis Of The Preferential Allocation Of Shares In Initial Public Offerings , Sean J. Griffith

Faculty Scholarship

This Article investigates the preferential allocation, or “spinning,” of shares in initial public offerings. It begins by examining the offering process and the incentives of underwriters, issuers, and investors. Through this examination of the participants and the process, it locates the harm of spinning in the underpricing of initial public offerings. The Article then seeks to identify precisely which participants in the offering process are harmed by the practice and finally evaluates the most appropriate means of addressing this harm.

Experimental Mycobacteriosis In Striped Bass Morone Saxatilis, D. T. Gauthier, M. W. Rhodes, W. K. Vogelbein, H. Kator, C. A. Ottinger

Biological Sciences Faculty Publications

Striped bass Morone saxatilis were infected intraperitoneally with approximately 10⁵ Mycobacterium marinum, M. shottsii sp. nov., or M. gordonae. Infected fish were maintained in a flow-through freshwater system at 18 to 21°C, and were examined histologically and bacteriologically at 2, 4, 6, 8, 17, 26, 36 and 45 wk post-infection (p.i.). M. marinum caused acute peritonitis, followed by extensive granuloma development in the mesenteries, spleen and anterior kidney. Granulomas in these tissues underwent a temporal progression of distinct morphological stages, culminating in well-circumscribed lesions surrounded by normal or healing tissue. Mycobacteria were cultured in high numbers from …

Enhancement Of Disease And Pathology By Synergy Of Trichuris Suis And Campylobacter Jejuni In The Colon Of Immunologically Naive Swine, Linda S. Mansfield, David T. Gauthier, Sheila R. Abner, Kathryn M. Jones, Stacey R. Wilder, Joseph F. Urban

Biological Sciences Faculty Publications

Campylobacter jejuni, a leading cause of bacterial gastroenteritis, has different age distribution and disease expression in developing and developed countries, which may be due to the endemnicity of infection and the age of acquisition of immunity. Differences in disease expression are not solely dependent on the C jejuni strain or virulence attributes. Another modulating factor in developing countries may be endemic nematode infections such as Trichuris, which drive type 2 cytokine responses and down-regulate type I immune responses. In this study, three-day-old germfree pigs given dual infections with Trichuris suis and C jejuni had more frequent, more severe …

'Practicing The New Creation: Wesley's Eschatalogical Community Formed By The Means Of Grace, Dean G. Blevins

The Asbury Journal

No abstract provided.

Performance Evaluation Of Sip Based Services In Umts, Maria Isabel Pous Fenollar

Theses

With ever increasing penetration of IP technologies and the tremendous growth in wireless data traffic, the wireless industry is evolving the mobile core networks towards IP technology. The 3rd Generation Partnership Project (3GPP) is specifying an IP Multimedia Core Network Sub-System (IMS) in UMTS Release 5 and Release 6, which is adjunct to the UMTS GPRS core network. This IP-based network will allow mobile operators to provide commonly used Internet applications to wireless user. The UMTS IMS uses the Internet Engineering Task Force (IETF) defined text based Session Initiation Protocol (SIP), to control a wide range of anticipated IP-based services …

Justice Lewis F. Powell, Jr. And The Counterrevolution In The Federal Securities Laws, Adam C. Pritchard

Articles

The confirmation of Lewis F. Powell, Jr., to the Supreme Court coincided with a dramatic shift in the Court's approach to securities law. This Article documents Powell's influence in changing the Court's direction in securities law. Powell's influence was the product of his extensive experience with the securities laws as a corporate lawyer, which gave him much greater familiarity with that body of law than his fellow Justices had. That experience also made him skeptical of civil liability, particularly class and derivative actions. Powell's skepticism led him to interpret the securities law in a consistently narrow fashion to reduce liability …