Digital Commons Network^™

Cloning Of Hbsag-Encoded Genes In Different Vectors And Their Expression In Eukaryotic Cells, Shan Qin, Hong Tang, Lian-San Zhao, Fang He, Yong Lin, Li Liu, Xiao-Mei He

Faculty Bibliography 2000s

AIM: To compare the efficiency of different plasmids as DNA vectors by cloning three HBsAg-encoded genes into two eukaryotic expression vectors, pRc/CMV and pSG5UTPL/Flag, and to express HBsAg S, MS, and LS proteins in SP2/0 cells, and to establish monoclone SP2/0 cell strains that are capable of expressing S or S2S proteins stably. METHODS: Segments of S, preS2-S, preS1-preS2-S genes of Hepatitis B virus were amplified by routine PCR and preS1-S fragment was amplified by Over-Lap Extension PCR. The amplified segments were cleaved with restricted endonuclease Hind III/Not I followed by ligation with pRc/CMV, or BamHI/EcoR I followed by ligation …

Dependence Of Two-Photon-Absorption-Excited Fluorescence On The Angle Between The Linear Polarizations Of Two Intersecting Beams, Alexandra Rapaport, Ferenc Szipocs, Michael Bass

Faculty Bibliography 2000s

We report experimental observation of the dependence of the fluorescence excited by two-photon absorption (TPA/E fluorescence) in dye solutions on the angle between the linear polarizations of two intersecting pump beams. Both degenerate and nondegenerate TPA/E fluorescence were studied with 4 ns pulsed tunable lasers as pump sources. We find that earlier theoretical analyses do not contain all of the observed changes in fluorescence signal with relative polarization angle.

Limiting Aspect Ratios Of Sagnac Interferometers, R. Glenn Sellar, Glenn D. Boreman

Faculty Bibliography 2000s

Any two-beam interferometer may be employed as a Fourier transform spectrometer. The two most commonly used for Fourier transform spectrometry are the Michelson interferometer and the Sagnac interferometer, the relative merits of which have been discussed in the literature. Typically, it is the interferometer that limits the acceptable range of angles for the input beam, and this maximum acceptance angle in turn limits the etendue, and hence limits the responsivity of the instrument when viewing an extended source. In designs where the interferometer is in a diverging or converging beam, the allowable range of input angles limits the focal ratio …

Attenuation Of Mycobacterium Tuberculosis By Disruption Of A Mas-Like Gene Or A Chalcone Synthase-Like Gene, Which Causes Deficiency In Dimycocerosyl Phthiocerol Synthesis, Tatiana D. Sirakova, Vinod S. Dubey, Michael H. Cynamon, Pappachan E. Kolattukudy

Faculty Bibliography 2000s

Tuberculosis is one of the leading preventable causes of death. Emergence of drug-resistant tuberculosis makes the discovery of new targets for antimyrobacterial drugs critical. The unique mycobacterial cell wall lipids are known to play an important role in pathogenesis, and therefore the genes responsible for their biosynthesis offer potential new targets. To assess the possible role of some of the genes potentially involved in cell wall lipid synthesis, we disrupted a mas-like gene, msl7, and a chalcone synthase-like gene, pks10, with phage-mediated delivery of the disruption construct, in which the target gene was disrupted by replacement of an internal segment …

The Largest Open Reading Frame (Pks12) In The Mycobacterium Tuberculosis Genome Is Involved In Pathogenesis And Dimycocerosyl Phthiocerol Synthesis, Tatianna D. Sirakova, Vinod S. Dubey, Hwa-Jung Kim, Michael H. Cynamon, Pappachan E. Kolattukudy

Faculty Bibliography 2000s

The cell wall lipids in Mycobacterium tuberculosis are probably involved in pathogenesis. The largest open reading frame in the genome of M. tuberculosis H37Rv, pks12, is unique in that it encodes two sets of domains needed to produce fatty acids. Apks12-disrupted mutant was produced, and disruption was confirmed by both PCR analysis and Southern blotting. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis showed that a 430-kDa protein band present in the wild type was missing in the mutant. Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MS) and liquid chromatography (LC)-MS analysis of tryptic peptides showed that 54 peptides distributed …

Pore Size Distribution, Survival Probability, And Relaxation Time In Random And Ordered Arrays Of Fibers, Manolis M. Tomadakis, Teri J. Robertson

Faculty Bibliography 2000s

We present a random walk based investigation of the pore size probability distribution and its moments, the survival probability and mean survival time, and the principal relaxation time, for random and ordered arrays of cylindrical fibers of various orientation distributions. The dimensionless mean survival time, principal relaxation time, mean pore size, and mean square pore size are found to increase with porosity, remain practically independent of the directionality of random fiber beds, and attain lower values for ordered arrays. Wide pore size distributions are obtained for random fiber structures and relatively narrow for ordered square arrays, all in very good …

Eigenmodes Of A Reflective Twisted-Nematic Liquid-Crystal Cell, Xinyu Zhu, Qi Hong, Yuhua Huang, Shin-Tson Wu

Faculty Bibliography 2000s

The eigenmodes of a reflective twisted-nematic liquid-crystal (TN LC) cell are analyzed based on the Jones matrix method. Two models are developed to describe the LC system in the low- and high-voltage regimes. The simulated transmission spectra of LC Fabry-Perot etalons are used to investigate the eigenmodes. In a general reflective TN LC cell, the eigenmodes are two orthogonal linear polarization states. Under some specific conditions, these two linear polarization states will approach the bisector or orthogonal to the bisector of the TN LC cell. This bisector effect is useful for reducing the operating voltage and enhancing the contrast ratio …

A Renewal Of The Sacred Heart University Library: A New Role For The Sacred Heart University Library, Dennis C. Benamati

Librarian Publications

It is a pleasure to present this prospectus for renewal of University’s Library to the students, faculty, administration, alumni and friends of Sacred Heart University. This first part of a two-part plan is intended to provide a statement of how we will proceed toward transforming the Library into a center of learning. But this is only the starting point for a larger and more exciting plan. Part II will outline a new role for the Library—one befitting a 21st century university—that we believe will distinguish Sacred Heart University among its peers. One that, we hope, will contribute to the University’s …

The Legacy Of Buckley V. Valeo, Joel Gora

Faculty Scholarship

No abstract provided.

H. Wayne Huizenga School Of Business And Entrepreneurship 2003-2004 Catalog, Nova Southeastern University

Huizenga Postgraduate Course Catalogs

No abstract provided.

Selected Dhs Data On 10–14-Year-Olds: Ghana, Population Council

Poverty, Gender, and Youth

Annex to "Facts about adolescents from the Demographic and Health Survey—Statistical tables for program planning"

Is 372.02: Management Of Telecommunications, Robin Moeur

University of Montana Course Syllabi

No abstract provided.

Is 372.01: Management Of Telecommunications, Robin Moeur

University of Montana Course Syllabi

No abstract provided.

Phar 110.80: The Use And Abuse Of Drugs, David S. Freeman

University of Montana Course Syllabi

No abstract provided.

Sw 200.01: Introduction To Social Work Practice, Charles R. Horejsi

University of Montana Course Syllabi

No abstract provided.

C&I 316.01: Children’S Literature And Critical Reading, Carolyn J. Lott

University of Montana Course Syllabi

No abstract provided.

C&I 318.02: Teaching Language Arts P-8, Steve Kalling

University of Montana Course Syllabi

No abstract provided.

C&I 571.01: Planning, Preparing, And Assessing Educational Technology Media, Sally Brewer

University of Montana Course Syllabi

No abstract provided.

C&I 427.01: Literacy Strategies For The Middle And Secondary School Content Areas, Marian J. Mckenna

University of Montana Course Syllabi

No abstract provided.

To Serve And Collect: Measuring Police Corruption, Sanja Kutnjak Ivkovic

Journal of Criminal Law and Criminology

No abstract provided.

Norms And Criminal Law, And The Norms Of Criminal Law Scholarship, Robert Weisberg

Journal of Criminal Law and Criminology

No abstract provided.

Pre-Arrest Silence: Minding That Gap Between Fourth Amendment Stops And Fifth Amendment Custody, Sara Ciarelli

Journal of Criminal Law and Criminology

No abstract provided.

Beyond Accommodation: Reconstructing The Insanity Defense To Provide An Adequate Remedy For Postpartum Psychotic Women, Jessie Manchester

Journal of Criminal Law and Criminology

No abstract provided.

Kidnapping Federalism: United States V. Wills And The Constitutionality Of Extending Federal Criminal Law Into The States, M. Todd Scott

Journal of Criminal Law and Criminology

No abstract provided.

Recent Books

Journal of Criminal Law and Criminology

No abstract provided.

The Relationship Between Public Perceptions Of Crime Seriousness And Support For Plea-Bargaining Practices In Israel: A Factorial Survey Approach, Sergio Herzog

Journal of Criminal Law and Criminology

No abstract provided.

Respect And The Fourth Amendment, Andrew E. Taslitz

Journal of Criminal Law and Criminology

No abstract provided.

Public Relations For Small Business: A Communication Application To Business Management, Diane J. Morton

Theses

Large corporations and businesses in the public eye employ public relations professionals or commission public relations agencies to manage efforts that protect business reputation and mediate relationships between a business and its publics. To succeed, small businesses need similar relationships with their publics, but many business owners do not dedicate time or resources to public relations functions. Relatively little information is available in the public relations literature specifically for small businesses. The purpose of this thesis is to identify the applicability of corporate public relations functions for small businesses, and to formulate a public relations plan for entrepreneurs and small …

Gender Differences Of College Students And Pathological Gambling, Richard Clyde Vantreece

Theses

Gender differences among college students and pathological gambling have been studied in the past by various researchers. The purpose of this study was to investigate which gender was more likely to be at risk for a pathological gambling disorder. This population is interesting for the purpose of this study because the majority of pathological gamblers report beginning this habit in adolescence. Fifty college students were selected from the Human Subject Research Pool Department. Students completed a demographic sheet and the South Oaks Gambling Screen. T-tests were done to compare the differences among the genders. The results showed no significant difference …

"People Just Don't Seem To Be Listening": A Case For A Functional Approach To Reputation Management, Stephen E. Gustafson

Theses

This paper focuses on the growing study of corporate reputation management and the potential benefits of focusing management attention on building, sustaining, and defending the corporate brand's reputation. Specifically, the purpose of the paper is to provide a logical justification for major corporations to add the role of chief reputation officer to their organizational structures in order to properly manage this significant intangible asset.

Secondary research of print and electronic news media coverage was conducted to document a case history of the reputation crisis and ultimate demise of Big Five accounting firm Andersen (more commonly known as Arthur Andersen). The …