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2003

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The Effects Of Intra-Articular Silicone Polymer On Synovial Fluid, Synovial Tissue, And Articular Cartilage In The Equine Middle Carpal Joint, Marilyn Rumbaugh Jan 2003

The Effects Of Intra-Articular Silicone Polymer On Synovial Fluid, Synovial Tissue, And Articular Cartilage In The Equine Middle Carpal Joint, Marilyn Rumbaugh

LSU Master's Theses

There is anecdotal documentation that intra-articular administration of liquid silicone is used as a lubricant for equine joints. Effects of silicone polymer on equine articular cartilage and synovial fluid have not been evaluated. The objectives were to characterize the effects of intra-articular administration of liquid silicone polymer on clinical, synovial fluid, and microscopic features in normal middle carpal joints of horses, and to determine if silicone migrates to the axillary lymph nodes. Eight adult horses were used in this study. Two mLs of 1,000 centistoke, medical-grade silicone was injected into one randomly selected middle carpal joint. The opposite middle carpal …


From Gamete Collection To Database Development: Development Of A Model Cryopreserved Germplasm Repository For Aquatic Species With Emphasis On Sturgeon, William Rittenhouse Wayman Jan 2003

From Gamete Collection To Database Development: Development Of A Model Cryopreserved Germplasm Repository For Aquatic Species With Emphasis On Sturgeon, William Rittenhouse Wayman

LSU Doctoral Dissertations

Development of a cryopreserved sperm repository could greatly benefit the study and recovery of endangered species. Currently, a national repository for endangered aquatic species does not exist. This dissertation addressed the development of methods for the creation of a model germplasm repository, which included methods to reduce bacterial contamination within sperm samples, verify sample identification, store sperm (refrigerated or cryopreserved), evaluate sperm quality before and after thawing, and maintain sample and broodstock information. Although these methods were developed for sturgeon, they could be adapted for any aquatic species. Accurate species identification of sperm samples is essential for an archival repository. …


Biomechanical Reconstruction Of The Appendicular Skeleton In Three North American Jurassic Sauropods, Ray Wilhite Jan 2003

Biomechanical Reconstruction Of The Appendicular Skeleton In Three North American Jurassic Sauropods, Ray Wilhite

LSU Doctoral Dissertations

Forelimb and hindlimb articulation, reconstructed musculature, and function were examined in Apatosaurus, Diplodocus, and Camarasaurus. A technique was developed using a Microscribe three-dimensional digitizer to capture external morphological data for skeletal reconstruction and determination of taxonomically useful features. The appendicular musculature of Alligator mississippiensis was dissected to determine form, function, origin, and insertion of muscles to aid in reconstruction of sauropod musculature. Contrary to the literature, M. caudofemoralis longus was found to originate primarily from the lateral surfaces of the proximal chevrons instead of the bodies and transverse processes of the caudals, demonstrating that chevron morphology is indicative of the …


The Effect Of Aging On The Immune Response To Vaccination In The Horse, Daniel H. Fermaglich Jan 2003

The Effect Of Aging On The Immune Response To Vaccination In The Horse, Daniel H. Fermaglich

LSU Master's Theses

Vaccination programs are designed to protect an animal from infection, however, depending upon the age and health of the animal vaccination may not stimulate a protective humoral response. It is possible that, as in the human and mouse models, geriatric equines may be less responsive than their younger counterparts to current vaccination protocols. The purpose of this study was to identify an age related diminution in the primary and secondary immune responses of geriatric horses in response to vaccination. Two groups of horses were sampled. The first group consisted of an open herd of 39 privately owned horses, varying in …


Psychosocial Predictors Of Visceral Adiposity, Paula C. Rhode Jan 2003

Psychosocial Predictors Of Visceral Adiposity, Paula C. Rhode

LSU Doctoral Dissertations

Psychosocial factors are thought to influence health through primarily direct physiological mechanisms or the alteration of health related behaviors. Three factors hypothesized to negatively impact health include arousal, life stress, and depressive symptomatology. One recent theorist suggests that the interaction between psychological stress and stress hormones on the neuroendocrine system may result in adverse changes to body composition, most notably the increased deposition of visceral adipose tissue (Bjorntorp, 1993). The current study prospectively examined the relationship between self-reported stressful life events, depressive symptoms and trait arousal on the deposition of visceral fat, as measured by computerized tomography (CT). Subjects were …


Macroinvertebrate Abundance And Distribution Of Hydrilla And Ceratophyllum Habitats In The Atchafalaya River Basin, Louisiana, Jose Checo Colon-Gaud Jan 2003

Macroinvertebrate Abundance And Distribution Of Hydrilla And Ceratophyllum Habitats In The Atchafalaya River Basin, Louisiana, Jose Checo Colon-Gaud

LSU Master's Theses

Submerged aquatic vegetation (SAV) plays an important role in aquatic systems, providing shelter, breeding habitat, and epiphytic forage for numerous fishes and aquatic macroinvertebrates. Since 1960, many lentic habitats in the southern U.S. have been invaded by Hydrilla verticillata, and in the last two decades this aggressive macrophyte has become the dominant species of SAV in the Atchafalaya River Basin in south central Louisiana. Because of its highly branched morphology and characteristically high densities, I found it difficult to quantitatively sample the macrofauna inhabiting hydrilla stands with traditional gears such as sweep nets, particularly under the canopy. As a consequence, …


Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul Jan 2003

Proportional Assessment Of X And Y Chromosome-Bearing Spermatozoa In Bull And Boar Ejaculates Using Conventional And Real-Time Pcr Techniques, Julius Braden Paul

LSU Doctoral Dissertations

Considerable variation in the percentage of Y-chromosome bearing spermatozoa (%Y-CBS) between ejaculates in the same male has been reported. Collection regime has been speculated to influence the degree of variation in %Y-CBS in bull ejaculates (Chandler et al., 1998). Experiments were designed to evaluate the effects of two collection regimes on %Y-CBS. Conventional PCR combined with gel electrophoresis and image analysis was employed to determine %Y-CBS in ejaculates from two bulls collected on 7-day intervals and two bulls on 21-day intervals. Real-time PCR technology was used to quantify %Y-CBS in the same ejaculates. Boar ejaculates were also analyzed with both …


High Carbon Dioxide Requiring Mutants Of Chlamydomonas Reinhardtti, Steve Vincent Pollock Jan 2003

High Carbon Dioxide Requiring Mutants Of Chlamydomonas Reinhardtti, Steve Vincent Pollock

LSU Doctoral Dissertations

Chlamydomonas reinhardtii is a photosynthetic alga that has the ability to concentrate CO2 around Rubisco to achieve enhanced rates of photosynthesis in a low CO2 environment. This dissertation presents results obtained from the generation and analysis of four high CO2 requiring mutants of C. reinhardtii. The use of reverse genetics is a very powerful tool to dissect out the individual components of metabolic pathways. Two reverse genetics methods were utilized in this study: a random insertional mutagenesis method to discover genes that are required for growth in a low CO2 environment, and a directed mutagenesis approach, RNA interference, to determine …


The Epidemiology Of Shiga-Toxigenic Escherichia Coli O157:H7 In Louisiana Cattle, And White-Tailed Deer, John Robert Dunn Jan 2003

The Epidemiology Of Shiga-Toxigenic Escherichia Coli O157:H7 In Louisiana Cattle, And White-Tailed Deer, John Robert Dunn

LSU Doctoral Dissertations

Shiga-toxigenic Escherichia coli (STEC) O157:H7 is an important human pathogen. Dairy cattle, beef cattle, and white-tailed deer (WTD) are involved in STEC O157:H7 transmission to humans. We conducted cross-sectional studies in Louisiana, using sensitive microbiological methods, in dairy cattle, beef cattle, and WTD to investigate the epidemiology of STEC O157:H7. Results of the dairy studies are as follows. In the point prevalence study, summer-time prevalence in herds was 38.5%, with a cow-level prevalence of 6.5%. Among positive herds, cow-level prevalence ranged from 3%-34.6%. Three of five herds sampled in the longitudinal study were positive. Cow-level prevalence increased during spring and …


The Invasive Tropical Shrub Clidemia Hirta (Melastomataceae) In Its Native And Introduced Ranges: Tests Of Hypotheses Of Invasion, Saara Jennie Dewalt Jan 2003

The Invasive Tropical Shrub Clidemia Hirta (Melastomataceae) In Its Native And Introduced Ranges: Tests Of Hypotheses Of Invasion, Saara Jennie Dewalt

LSU Doctoral Dissertations

Exotic pest plants often grow to greater stature, become more abundant, and display increased shade tolerance in their introduced ranges than in their native ranges. These differences have been hypothesized to result from genetic shifts in biomass allocation, growth, or photosynthesis between genotypes in native and introduced ranges or from plastic, phenotypic responses to different environmental conditions, such as lower herbivore or fungal pest loads in areas of introduction. I used the tropical shrub Clidemia hirta (Melastomataceae) as a model exotic pest plant to test these two non-mutually exclusive hypotheses of invasion. Clidemia hirta invades forest understory and is more …


Will The Real Jesus Please Stand Up?: Bridging The Divide Between The Jesus Seminar And Its Opponents Through A Burkeian Approach, Carol Melissa Hopson-Sparks Jan 2003

Will The Real Jesus Please Stand Up?: Bridging The Divide Between The Jesus Seminar And Its Opponents Through A Burkeian Approach, Carol Melissa Hopson-Sparks

LSU Doctoral Dissertations

This study employs a Burkeian cluster-agon analysis approach to analyze the rhetoric of four members of the Jesus Seminar; namely, Robert Funk, Marcus Borg, John Dominic Crossan, and John Shelby Spong as well as that of two of the Jesus Seminar’s critics; Luke Timothy Johnson and N. Thomas Wright. Specifically, this study sought to discern the orientations or perspectives held by each of the examined rhetors in an effort to locate common ground or similar foundations within two seemingly disparate points of view. In doing so, this study creates a third perspective, or corrective, based on the orthopraxis approach of …


Optimization And Automation Of A Thermal Oyster Shucking Process, Daniel Edgar Martin Jan 2003

Optimization And Automation Of A Thermal Oyster Shucking Process, Daniel Edgar Martin

LSU Doctoral Dissertations

Louisiana Gulf Coast oysters (Crassostrea virginica) were subjected to thermal shucking treatments to effect adductor muscle release from both left and right valves. The oysters were instrumented with thermocouples to monitor and record process temperatures in the oysters and on the shell. Following treatment, the oysters were evaluated for relaxation and release of the adductor muscle, meat quality and texture, and for the effect of the treatments on the storage life of the oysters as measured by total microbial plate counts. The treatments of many oysters resulted in a complete release of the adductor muscle from the shell while maintaining …


Quantitative Inheritance Of Calpastatin Activity As An Assessment Measure For Meat Tenderness In Brahman Steers, Fuad Mohammad Tawfiq Odeh Jan 2003

Quantitative Inheritance Of Calpastatin Activity As An Assessment Measure For Meat Tenderness In Brahman Steers, Fuad Mohammad Tawfiq Odeh

LSU Doctoral Dissertations

Calpastatin activity at 24 h postmortem is inversely proportional to postmortem tenderization and accounts for a greater proportion of the variation in beef tenderness. Tenderness was determined by measurement of 24 h postmortem longissimus muscle calpastatin activity (CA) and Warner-Bratzler shear force after 7 and 14 d of steak aging. The quantitative inheritance of tenderness in Brahman steers was investigated phenotypically by Multiple Trait Derivative-Free Restricted Maximum Likelihood (MTDFREML) procedures and genotypically by using restriction fragment length polymorphisms (RFLP), a microsatellite marker, and DNA sequence analyses. In experiment 1, MTDFREML was used to obtain the genetic parameters of tenderness traits …


The Influence Of Selected Factors On Burnout Among Faculty In Higher Education, Ronald R. Rush Jan 2003

The Influence Of Selected Factors On Burnout Among Faculty In Higher Education, Ronald R. Rush

LSU Doctoral Dissertations

The purpose of this study was to explore demographic variables and job satisfaction and the relationship with burnout among higher education faculty. The sample included 248 tenured professors selected through simple random sampling techniques. A three-part instrument was used: the Maslach Burnout Inventory (MBI-ES), which measured levels of burnout based on subscales of Emotional Exhaustion, Depersonalization, and Personal Accomplishment; the Job Descriptive Index (JDI) and Job in General (JIG) scales, which indicated job satisfaction in areas of: Work on Present Job, Present Pay, Opportunities for Promotion, Supervision on Present Job, People in Present Job, and Job in General; and, a …


Developing Landscape-Scaled Habitat Selection Functions For Forest Wildlife From Landsat Data: Judging Black Bear Habitat Quality In Louisiana, Robert Owen Wagner Jan 2003

Developing Landscape-Scaled Habitat Selection Functions For Forest Wildlife From Landsat Data: Judging Black Bear Habitat Quality In Louisiana, Robert Owen Wagner

LSU Doctoral Dissertations

Understanding habitat needs of animal populations is critical for their effective management. In recent years, technological advances have increased the range of methods available to examine habitat selection patterns. However, available habitat data are often either limited to small geographic areas or are of coarse resolution, resulting in a gap in data to model habitat selection at landscape scales. I explored a method of processing Landsat data, the at-satellite reflectance tasseled cap, to address this data gap using black bears in south central Louisiana as a case study. As I showed, this case was particularly instructive because these bears occupy …


As The Ravens Watch: Exploring The Oracle, Kathryn Lavender Hunter Jan 2003

As The Ravens Watch: Exploring The Oracle, Kathryn Lavender Hunter

LSU Master's Theses

I believe in the process in which the artist never works alone but always in collaboration with the elements of chance. As an artist I live and work everyday collaborating with chance, from reading my cards to creating ethereal birds. As I sew pieces together, the thread becomes another element of the image by chance, stitches to lines, lines to pattern. Chance leads the birds to portraying their own personality as they are each created, one’s head tilts to the right while another sits tall with dignity. This work is about the connection to the subconscious, my connection to it …


A Role For Constitutive Pathogen Resistance5 In Promoting Cell Expansion In Arabidopsis Thaliana, Ginger Marie Brininstool Jan 2003

A Role For Constitutive Pathogen Resistance5 In Promoting Cell Expansion In Arabidopsis Thaliana, Ginger Marie Brininstool

LSU Doctoral Dissertations

Arabidopsis thaliana is a model organism used for genetic, molecular, and biochemical analyses of higher plant processes. Leaf hairs (trichomes) on the surface of Arabidopsis leaves are macroscopic single cells that develop by elaborate cell expansion and differentiation of the epidermis. Arabidopsis trichomes provide an excellent model to study plant cell expansion and differentiation and the cell cycle. Upon maturation, an Arabidopsis trichome becomes a branched, stellate structure with a highly birefringent cell wall. Arabidopsis mutants have been isolated that have trichomes that look glassy and have reduced birefringence. CONSTITUTIVE PATHOGEN RESISTANCE5 (CPR5) is a previously isolated Arabidopsis gene observed …


Imagining Corporate Culture: The Industrial Paternalism Of William Hesketh Lever At Port Sunlight, 1888-1925, Jeremy David Rowan Jan 2003

Imagining Corporate Culture: The Industrial Paternalism Of William Hesketh Lever At Port Sunlight, 1888-1925, Jeremy David Rowan

LSU Doctoral Dissertations

At Lever Brothers soap company in Port Sunlight, U.K., William Lever, between 1888-1925, instituted employee benefits that preceded the welfare state. Yet, in addition to providing tangible benefits for the employees (including free medical care, pensions, an employee profit-sharing scheme), Lever also created a strong corporate identity for his employees by cultivating a strong company and personal image, one constructed in response to national discourses surrounding industrialization, empire, national identity, and economic decline. Lever offered his company as a solution to national concerns and thus posited his workers as participants in patriotic efforts and empire-building. He forged an effective company …


Perspectives On Comparative Literature, Alexandru Boldor Jan 2003

Perspectives On Comparative Literature, Alexandru Boldor

LSU Master's Theses

The main objective of this dissertation was to provide researchers interested in the history and evolution of "comparative literature" with a collection of references delineating the evolution of the concept and the development of academic departments dedicated to its study. The paper includes a first section describing the main issues contributing to the "identity crisis" with which studies and departments defining themselves as "comparative" were consistently confronted ever since the term was coined. The "preliminary concepts" section offers an overview of the elements that usually confer a "comparative" quality to a literary study, such as interdisciplinarity and multiculturalism, together with …


"Science In Skirts": Representations Of Women In Science In The "B" Science Fiction Films Of The 1950s, Bonnie Noonan Jan 2003

"Science In Skirts": Representations Of Women In Science In The "B" Science Fiction Films Of The 1950s, Bonnie Noonan

LSU Doctoral Dissertations

This project shows how central representations of women in science were to the “B” science fiction films of the 1950s and uses these films as valuable indicators for cultural analysis. I argue that the emergence of the modern American science fiction film in 1950 combined with the situation of post-W.W.II women in science to create a genre explicitly amenable to exploring the tension between a woman’s place in the home and her place in the work force, particularly in the fields of science. Out of a context of 114 “B” science fiction films produced between 1950 and 1966, I offer …


Louisiana Sugar: A Geohistorical Perspective, Elizabeth Vaughan Jan 2003

Louisiana Sugar: A Geohistorical Perspective, Elizabeth Vaughan

LSU Doctoral Dissertations

The planting of sugarcane in Louisiana’s southern parishes has persisted with stunning continuity since its introduction in the late eighteenth century. This industry, however, is an economic and agricultural anomaly. It is a relic of the sixteenth-century expansion of European capitalism in which granulated sugar, then a novel product, stimulated the Atlantic slave trade and contributed to the incorporation of the sugar-producing colonies of the Americas into an emerging European-world economy. The Louisiana sugar industry was launched in 1795 with a historic granulation from a new variety of sugarcane recently introduced into the Caribbean. From this early success, the industry …


Modification Of The Nucleotide Cofactor-Binding Site Of Cytochrome P450 Reductase To Enhance Turnover With Nadh In Vivo, Calvin Lee Elmore Jan 2003

Modification Of The Nucleotide Cofactor-Binding Site Of Cytochrome P450 Reductase To Enhance Turnover With Nadh In Vivo, Calvin Lee Elmore

University of Kentucky Doctoral Dissertations

NADPH-cytochrome P450 reductase is the electron transfer partner for the cytochromes P450, heme oxygenase, and squalene monooxygenase, and is a component of the nitric oxide synthases and methionine synthase reductase. P450 reductase shows very high selectivity for NADPH and uses NADH only poorly. Substitution of tryptophan 677 with alanine (W677A) has been shown by others to yield a 3-fold increase in turnover with NADH, but profound inhibition by NADP+ makes the enzyme unsuitable for in vivo applications. In the present study site-directed mutagenesis of amino acids in the 2'-phosphate-binding site of the NADPH domain, coupled with the W677A substitution, was …


Reticent Romans: Silence And Writing In La Vie De Saint Alexis, Le Conte Du Graal, And Le Roman De Silence, Evan J. Bibbee Jan 2003

Reticent Romans: Silence And Writing In La Vie De Saint Alexis, Le Conte Du Graal, And Le Roman De Silence, Evan J. Bibbee

LSU Doctoral Dissertations

Apart from discourse and yet somehow part of it, silence is a powerfully ambiguous linguistic phenomenon that blurs the lines between presence and absence. Eluding the material aspects of oral and written language, it is only perceptible as the gaps or spaces between words. Nonetheless, it plays a role in all linguistic productions: although silence itself cannot be directly communicated, it can influence communication. In a literary text, silence may takes on many different guises, including rhythmic hesitations, rhetorical omissions, and poetic oppositions that mimic the audible gaps of spoken language. The visual, aural, and fictional interaction of all these …


Perceptions Of Stereotypes In Hispanic Children's Literature, Nancy Gomez Jan 2003

Perceptions Of Stereotypes In Hispanic Children's Literature, Nancy Gomez

LSU Doctoral Dissertations

This study attempted to determine the accurateness of the representation of the Hispanic culture in children’s books. I interviewed ten people: five non-Hispanic and five Hispanic, and I found that the Hispanic people do not seem to pay as much attention to physical features as non-Hispanic people do. However, they were concerned about the portrayal of the Hispanic culture in traditional ways: the traditional roles of women, the traditional dress, the architecture of the houses and the portrayal of the Hispanic people living in rural areas and being extremely poor. It appears that from the timeline covered by the books, …


Heavy Smokers Choose Large, Immediate Rewards With Large Penalties On A Simulated Task Of Gambling, Michael Shawn Businelle Jan 2003

Heavy Smokers Choose Large, Immediate Rewards With Large Penalties On A Simulated Task Of Gambling, Michael Shawn Businelle

LSU Master's Theses

The Gambling Task is a complex neuropsychological test (in the form of a card game) that examines the ability of individuals to assess potential immediate gains over long-term losses. Gambling Task performance has been examined in previous studies with individuals who are dependent on alcohol, cocaine, heroin, and amphetamine. These studies have shown that those who are dependent on the aforementioned substances perform more poorly on the Gambling Task than controls. Specifically, in relation to controls, drug/alcohol dependent individuals show impairment by tending to pick more cards that have large immediate gains and very large delayed punishers. The delayed punisher …


Population Structure And Genetics Of Longevity In A Colony Of Dog Guides, John B. Cole Jan 2003

Population Structure And Genetics Of Longevity In A Colony Of Dog Guides, John B. Cole

LSU Doctoral Dissertations

The objectives of this study were the description of changes in genetic diversity in a colony of dog guides since its founding, and the investigation of the genetics of longevity in that population. Two breeds of dog, German Shepherds (GS) and Labrador Retrievers (LR), were evaluated. There were rapid increases in average pairwise relationship in both breeds, although the average was approximately one-third higher in the GS population than in the LR population. A similar trend was observed for average inbreeding. In the current generation, relationship and inbreeding for all animals averaged 25.3% and 26.2% in GS and 15.5% and …


The History Of Russian Vaudeville From 1800 To 1850, Alexander V. Tselebrovski Jan 2003

The History Of Russian Vaudeville From 1800 To 1850, Alexander V. Tselebrovski

LSU Doctoral Dissertations

 There is no significant scholarly work on the history of the Russian vaudeville. The author of the dissertation makes an attempt to explore the history of vaudeville in Russia from 1812, when the first original vaudeville was written by A. Shakhovskoi, to the 1850s, when vaudeville as a genre was finalized as a form and brought to its classic completion. Two phases of the history of vaudeville in Russia, aristocratic and democratic-raznochinnyi, are considered in close connection with the political, social, and cultural events of Russian society of the time. The first phase embraces the period from 1812, when …


Using Smart Growth Principles For Development In St. Landry Parish, Rebecca L. Scheffler Jan 2003

Using Smart Growth Principles For Development In St. Landry Parish, Rebecca L. Scheffler

LSU Master's Theses

Since the end of World War II, many American cities have seen an outward migration from their core into open spaces along the city’s edge. The urban/suburban sprawl phenomenon not only affects urban cores but the rural lands and open spaces surrounding them. As landscape architects, we study humans and their interactions with the environment and have an important position in making informed decisions about issues of sprawl and unmitigated growth in communities. This study was conducted to provide city and parish officials and planning boards with options for beneficial growth in St. Landry Parish, a rural parish in south …


The Attack Will Go On The 317th Infantry Regiment In World War Ii, Dean James Dominique Jan 2003

The Attack Will Go On The 317th Infantry Regiment In World War Ii, Dean James Dominique

LSU Master's Theses

The 317th Infantry Regiment was reactivated on July 15, 1942 as a subordinate element of the 80th Infantry Division. The regiment trained for two years in Tennessee, Kansas, Arizona, California, and finally New Jersey before departing for England in June 1944. Entering the European continent after D-Day, the regiment experienced its first combat in August 1944 when it assisted in closing the gap at Falaise and spearheaded Third Army's attack on Nancy. The 317th sat through the logistics shortfall that stopped the Third Army's advance during the "October Pause" in the fall of 1944. But then in November the regiment …


Establishment Of A Callus Culture And Measurement Of Seasonal Changes In Secondary Compound Production In Eucommia Ulmoides Oliver, Erica Deshay Gray Jan 2003

Establishment Of A Callus Culture And Measurement Of Seasonal Changes In Secondary Compound Production In Eucommia Ulmoides Oliver, Erica Deshay Gray

LSU Master's Theses

Eucommia ulmoides Oliver (Eucommiaceae) contains many secondary metabolites, including the lignan pinoresinol di-O-β] glucoside (PG) and the iridoid glycoside aucubin (AU), which have been shown to have anti-hypertensive, antioxidant, and hepatoprotective biological properties. The main objective of this research was to create a new agricultural crop in Louisiana for maintaining healthy blood pressure levels. This was done by: 1) determining the seasonal changes in the concentrations of PG and AU in four-year-old E. ulmoides trees in cultivation in Baton Rouge, Louisiana using Thin Layer Chromatography and High Pressure Liquid Chromatography (HPLC) and 2) determining production of secondary metabolites of E. …