Digital Commons Network^™

Immobilization Of Swift Foxes With Ketamine Hydrochloride-Xylazine Hydrochloride, Rebecca L. Telesco, Marsha A. Sovada

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

There is an increasing need to develop field immobilization techniques that allow researchers to handle safely swift foxes (Vulpes velox) with minimal risk of stress or injury. We immobilized captive swift foxes to determine the safety and effectiveness of ketamine hydrochloride and xylazine hydrochloride at different dosages. We attempted to determine appropriate dosages to immobilize swift foxes for an adequate field-handling period based on three anesthesia intervals (induction period, immobilization period, and recovery period) and physiologic responses (rectal temperature, respiration rate, and heart rate). Between October 1998–July 1999, we conducted four trials, evaluating three different dosage ratios of …

Discounting And Reciprocity In An Iterated Prisoner’S Dilemma, David W. Stephens, Colleen M. Mclinn, Jeffrey R. Stevens

Jeffrey Stevens Publications

The Iterated Prisoner’s Dilemma (IPD) is a central paradigm in the study of animal cooperation. According to the IPD framework, repeated play (repetition) and reciprocity combine to maintain a cooperative equilibrium. However, experimental studies with animals suggest that cooperative behavior in IPDs is unstable, and some have suggested that strong preferences for immediate benefits (that is, temporal discounting) might explain the fragility of cooperative equilibria. We studied the effects of discounting and strategic reciprocity on cooperation in captive blue jays. Our results demonstrate an interaction between discounting and reciprocity. Blue jays show high stable levels of cooperation in treatments with …

Influence Of Protein Tyrosine Kinases On Cell Volume Change-Induced Taurine Release, Herminia Pasantes-Morales, Rodrigo Franco

School of Veterinary and Biomedical Sciences: Faculty Publications

Taurine efflux occurs in association with cell swelling in both hyposmotic and isosmotic conditions and during cell shrinkage in apoptotic death. Release occurs through a leak pathway, is largely Ca²⁺-independent and is sensitive to Cl^– channel blockers. Taurine efflux elicited by hyposmolarity is reduced or suppressed by tyrosine kinase blockers and increased by tyrosine phosphatase inhibitors. The specific kinases involved are still unknown and may be different in the various cell types. Non-receptor and scr-related protein kinases have been identified in some cells as elements that directly phosphorylate the taurine efflux pathway. Possible tyrosine kinase targets are …

Methods And Compositions For Protection Against Bovine Viral Diseases, Subramaniam Srikumaran

School of Veterinary and Biomedical Sciences: Faculty Publications

The present invention relates to methods and compositions for eliciting an immune response against bovine viral epitopes. The methods comprise combining at least one heat shock protein with at least one bovine viral epitope to form a purified epitope/heat shock protein complex and administration of an immune system stimulating amount of the purified epitope/heat shock protein complex. The compositions comprise, a purified epitope/heat shock protein complex comprising at least one bovine viral epitope complexed with at least one heat shock protein, and a pharmaceutically acceptable carrier, diluent or excipient.

Cellular Tropisms And Co-Receptor Usage Of Hiv-1 Isolates From Vertically Infected Children With Neurological Abnormalities And Rapid Disease Progression, Micheline Mccarthy, Jun He, Denise Auger, Rebeca Geffin, Cecilia Hutto, Charles Wood, Gwendolyn Scott

Nebraska Center for Virology: Faculty Publications

The longitudinal evolution of HIV-1 phenotypes was studied in a cohort of six vertically infected children with early onset and rapid progression of clinical disease. Among 30 viral isolates obtained from peripheral blood, tropisms for both human blood-derived cells (macrophages, T-lymphocytes), and for human neural (brain-derived) cells (microglia, astrocytes) were determined, as was chemokine co-receptor usage. All children harbored from birth macrophage-tropic isolates using the CCR5 co-receptor. Two children later developed T-cell tropic isolates with CXCR4 and CCR3 usage. While all six patients developed neurological abnormalities, only three produced neural cell tropic isolates, which used CCR5. However, early and persistent …

Parelaphostrongylus Tenuis In Captive Pronghorn Antelope (Antilocapra Americana) In Nebraska, Heather A. Simmons, David J. Steffen, Douglas L. Armstrong, Douglas G. Rogers

School of Veterinary and Biomedical Sciences: Faculty Publications

Lesions in four captive pronghorn antelope (Antilocapra americana) naturally infected with Parelaphostrongylus tenuis in eastern Nebraska (USA) are described in this report. Animals were bright and alert with hind limb ataxia that progressed to sternal or lateral recumbency between July 28 and October 17, 1998. Animals were euthanized due to disease progression despite therapy. Multifocal decubital ulcers over bony prominences occurred in two animals and chronic unilateral otitis media was present in one animal. Histopathologic examination revealed severe Wallerian degeneration randomly scattered throughout the spinal cords of all four animals. Spinal cord sections from two animals contained adult …

Probability Of Nitrate Contamination Of Recently Recharged Groundwaters In The Conterminous United States, Bernard Nolan, Kerie Hitt, Barbara Ruddy

United States Geological Survey: Staff Publications

A new logistic regression (LR) model was used to predict the probability of nitrate contamination exceeding 4 mg/L in predominantly shallow, recently recharged groundwaters of the United States. The new model contains variables representing (1) N fertilizer loading (p < 0.001), (2) percent cropland-pasture (p < 0.001), (3) natural log of human population density (p < 0.001), (4) percent well-drained soils (p < 0.001), (5) depth to the seasonally high water table (p < 0.001), and (6) presence or absence of unconsolidated sand and gravel aquifers (p =0.002). Observed and average predicted probabilities associated with deciles of risk are well correlated (r² = 0.875), indicating that the LR model fits the data well. The likelihood of nitrate contamination is greater in areas with high N loading and well-drained surficial soils over unconsolidated sand and gravels. The LR model correctly predicted the status of nitrate contamination in 75% of wells in a validation data set. Considering all wells used in both calibration and …

Review Of Determination Of Organic Compounds In Soils, Sediments And Sludges By T. R. Crompton, John J. Johnston

United States Department of Agriculture Wildlife Services: Staff Publications

T. R. Crompton has accomplished the Herculean task of summarizing the literature (up to 1998) for the determination of organic and organometallic substances in soils, aquatic, and marine sediments and sludge. Although previous books address the analysis of such compounds in water, this is the first book dedicated to the analysis of pollutants in soils, sediments, and sludges, matrices that typically contain a variety of interfering compounds and offer significant analytical challenges. Overall, the book is relatively easy to read; the “camera ready” manuscript produced by the author contains clear tables, figures, and font. Classes of compounds addressed in this …

Safety Of Brucella Abortus Strain Rb51 Vaccine In Non-Target Ungulates And Coyotes, Terry J. Kreeger, Thomas J. Deliberto, Steven C. Olsen, William H. Edwards, Walter E. Cook

United States Department of Agriculture Wildlife Services: Staff Publications

Brucellosis is endemic in free-ranging elk (Cervus elaphus) and bison (Bison bison) in the Greater Yellowstone Area (GYA; USA). It is possible that an oral brucellosis vaccine could be developed and disseminated in the GYA to reduce disease transmission. Should this occur, non-target species other than elk and bison may come in contact with the vaccine resulting in morbidity or mortality. To assess biosafety, bighorn sheep (Ovis canadensis; n=10), pronghorn (Antilocapra americana; n=9), mule deer (Odocoileus hemionus; n=11), moose (Alces alces shirasi; n=10), and …

Wildlife Diseases: Crying Wolf Or Crying Shame?, Robert G. Mclean

United States Geological Survey: Staff Publications

Diseases of North American wildlife are causing serious problems for wildlife, and some wildlife diseases pose health threats to humans and domestic animals. Information will be presented at this wildlife disease session to alert resource managers enough to consider disease as an important issue when managing wildlife populations. It will be a crying shame if appropriate actions are not taken to monitor diseases adequately and to prevent or control them to protect our wildlife resources.

Growth Response Of Velvetleaf To Three Postemergence Herbicides, Cheryl A. Murphy, John L. Lindquist

Department of Agronomy and Horticulture: Faculty Publications

Knowledge of how reduction in the rate of herbicide application or rotation of their mode of action influences weed growth will provide insight into how successful these practices will be in an integrated weed management program. Field experiments were conducted in 1996 and 1997 to quantify velvetleaf growth response to three postemergence herbicides, each with a different mode of action. A monoculture of velvetleaf was treated with halosulfuron, dicamba, and flumiclorac at 0, 0.10, 0.25, 0.50, 0.75, and 1.0 X the labeled rate for weed control in corn. Percent plant mortality increased with rate of application; the greatest mortality occurred …

Combinatorial Control Of Yeast Fet4 Gene Expression By Iron, Zinc, And Oxygen, Brian M. Waters, David J. Eide

Department of Agronomy and Horticulture: Faculty Publications

Acquisition of metals such as iron, copper, and zinc by the yeast Saccharomyces cerevisiae is tightly regulated. High affinity uptake systems are induced under metal-limiting conditions to maintain an adequate supply of these essential nutrients. Low affinity uptake systems function when their substrates are in greater supply. The FET₄ gene encodes a low affinity iron and copper uptake transporter. FET₄ expression is regulated by several environmental factors. In this report, we describe the molecular mechanisms underlying this regulation. First, we found that FET₄ expression is induced in iron-limited cells by the Aft1 iron-responsive transcriptional activator. Second, FET …

Manure As Carbon Source For Soil Improvement And Crop Production: Site-Specific Application, Bahman Eghball, Daniel Ginting, Charles A. Shapiro, James S. Schepers, Christopher J. Bauer

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Manure, a renewable resource, is an excellent source of nutrients that can be substituted for synthetic types of fertilizers. The C in manure can enhance the physical and chemical properties of soils, especially infertile soils, as these soils typically contain low levels of organic matter and nutrients. The objective of this study was to evaluate the effects of C and nutrients in manure on soil C dynamics and crop production. The treatments included applications of site-specific manure (SSM), uniform manure (UM), uniform commercial fertilizer, and a no treatment check. Field strips 40 ft (16 corn rows) wide and 2200 ft …

Ft-Nir Spectroscopic Analysis Of Nitrogen In Cotton Leaves, Mark R. Riley, Loreto C. Canaves

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Near-infrared spectroscopy was evaluated as a means to quantify the nitrogen content- in fresh cotton leaves (Gossypium hirsutum L. var. Delta Pine 90) subjected to a factorial design experiment of varying nitrogen and water applications. Absorbance spectra were collected in the 10000-4000 cm^-1 (1000-2500 nm) region from fresh cotton leaves over a two month portion of the growing season. Total nitrogen content was quantified by a wet chemistry Kjeldahl method for validation purposes. Partial least-squares regression analysis, using an automated grid search method, selected the spectral region 6041 to 5651 cm^-1 (1650-1770 nm) for analysis based on having …

Valuing The Arts: A Contingent Valuation Approach, Eric Thompson, Mark Berger, Glenn Blomquist, Steven Allen

College of Business: Faculty Publications

Government funding of the arts has received considerable attention in the United States in recent years. Efforts to cut funding to the National Endowment for the Arts and declining budgets for state arts agencies have raised questions about how much individuals value the arts. This paper applies the contingent valuation method to assess this value, using surveys of random households and of arts patrons. Our analysis estimated a mean willingness to pay (WTP) among all Kentucky households from $6 to $27, depending on the estimation technique used and on whether the scenario discussed is to increase arts performances by 25 …

Rabd, A Dictyostelium Rab14-Related Gtpase, Regulates Phagocytosis And Homotypic Phagosome And Lysosome Fusion, Ed Harris, James Cardelli

Department of Biochemistry: Faculty Publications

RabD, a Dictyostelium Rab14-related GTPase, localizes in the endo-lysosomal pathway and contractile vacuole system of membranes. Cell lines expressing dominant-negative RabD were defective in endocytosis, endosomal membrane flow and homotypic lysosome fusion. In support of a role for RabD in fusion, cells overexpressing constitutively active RabD^Q67L accumulated enlarged hydrolase-rich acidic vesicles ringed with GFP-RabD, consistent with RabD directly regulating lysosome fusion. To determine whether RabD also regulated phagocytosis and/or homotypic phagosome fusion (a process stimulated by many intracellular pathogens), cells overexpressing dominant-active (RabD^Q67L) or dominant-negative (Rab^N121I) RabD were analyzed microscopically and biochemically. The rate of …

Selenoprotein R Is A Zinc-Containing Stereo-Specific Methionine Sulfoxide Reductase, Gregory V. Kryukov, R. Abhilash Kumar, Ahmet Koc, Zhaohul Sun, Vadim N. Gladyshev

Department of Biochemistry: Faculty Publications

Selenoprotein R (SelR) is a mammalian selenocysteine-containing protein with no known function. Here we report that cysteine homologs of SelR are present in all organisms except certain parasites and hyperthermophiles, and this pattern of occurrence closely matches that of only one protein, peptide methionine sulfoxide reductase (MsrA). Moreover, in several genomes, SelR and MsrA genes are fused or clustered, and their expression patterns suggest a role of both proteins in protection against oxidative stress. Consistent with these computational screens, growth of Saccharomyces cerevisiae SelR and MsrA mutant strains was inhibited, and the strain lacking both genes could not grow, in …

Life History Response Of Mediterranean Fruit Flies To Dietary Restriction, James R. Carey, Pablo Liedo, Lawrence G. Harshman, Ying Zhang, Hans-Georg Müller, Linda Partridge, Jane-Ling Wang

Lawrence G. Harshman Publications

The purpose of this study was to investigate medfly longevity and reproduction across a broad spectrum of diet restriction using a protocol similar to those applied in most rodent studies. Age-specific reproduction and age of death were monitored for 1,200 adult males and 1,200 females, each individually maintained on one of 12 diets from ad libitum to 30% of ad libitum. Diet was provided in a fixed volume of solution that was fully consumed each day, ensuring control of total nutrient consumption for every fly. Contrary to expectation and precedence, increased longevity was not observed at any level of …

Food Pulses Increase Longevity And Induce Cyclical Egg Production In Mediterranean Fruit Flies, James R. Carey, Pablo Liedo, Lawrence G. Harshman, X. Liu, Hans-Georg Müller, Linda Partridge, Jane-Ling Wang

Lawrence G. Harshman Publications

1. Inasmuch as virtually all studies on mortality and reproduction in insects are conducted under conditions in which food availability is constant, little is known about the demographic response of insects to variable food environments. For example, it is not known if and to what extent the life expectancy of insects subjected to shortages of high-quality food will increase and/or whether this increase is associated with major decreases in lifetime reproduction.

2. Therefore cohorts of 100 individual female medflies were subjected to different sets of conditions of protein availability (interspersed with sugar-only diets) including ad libitum sugar-only (no protein), ad …

A Comparative Study Of Geometric Rule Learning By Nutcrackers (Nucifraga Columbiana), Pigeons (Columba Livia), And Jackdaws (Corvus Monedula ), Juli E. Jones, Elena Antoniadis, Sara J. Shettleworth, Alan Kamil

Papers in Behavior in Biological Sciences

Three avian species, a seed-caching corvid (Clark’s nutcrackers; Nucifraga columbiana), a non-seed-caching corvid (jackdaws; Corvus monedula), and a non-seed-caching columbid (pigeons; Columba livia), were tested for ability to learn to find a goal halfway between 2 landmarks when distance between the landmarks varied during training. All 3 species learned, but jackdaws took much longer than either pigeons or nutcrackers. The nutcrackers searched more accurately than either pigeons or jackdaws. Both nutcrackers and pigeons showed good transfer to novel landmark arrays in which interlandmark distances were novel, but inconclusive results were obtained from jackdaws. Species differences in this …

Development Of The Muskox Lungworm, Umingmakstrongylus Pallikuukensis (Protostrongylidae), In Gastropods In The Arctic, Susan J. Kutz, Eric P. Hoberg, John Nishi, Lydden Polley

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Development of the muskox protostrongylid lungworm, Umingmakstrongylus pallikuukensis, in its slug intermediate host, Deroceras laeve, was investigated under field conditions in the Arctic. Every two weeks, from 19 June to 28 August 1997, groups of ten experimentally infected slugs were placed in tundra enclosures in a mesic sedge meadow near Kugluktuk, Nunavut, Canada. First-stage larvae (L1) infecting slugs on or before 17 July developed to third-stage larvae (L3) in 4–6 weeks. Intensity of L3 in slugs peaked at 6–8 weeks post infection (PI) and then progressively declined by 10, 12, and 48–50 weeks PI. Abundance of L3 in …

Implications Of Single-Particle Experiments For Track Theory, Therapy And Radiation Protection, Robert Katz, Francis Cucinotta

Robert Katz Publications

‘‘Because the predominant exposure of cells in humans is to single isolated tracks, a critical question is what effects a single track is capable of producing and with what probabilities.’’ ‘‘Most of the current biophysical models. . . make the clear prediction that a single track can produce virtually all of the detrimental stochastic effects of interest.’’ ‘‘But one model, in particular (the amorphous track model of Katz and coworkers), disagrees fundamentally that a single low LET track has the ability to cause the cellular changes.’’ ‘‘This model leads to very dramatic differences in the predicted risk at low doses. …

Recombinant Tissue Plasminogen Activator For Acute Ischemic Stroke, Amira R. Al-Buhairi, Mohammed M. Jan

Saudi Medical Journal

Stroke is a leading cause of serious and long-term disability and death worlwide, with approximately 750,000 strokes occuring annually in the United States of America. The risk of stroke doubles each decade for people over 55 years. Cerebral angiography conducted soon after the onset of stroke demonstrates arterial occlusion in 70%-80% of cases. Recanalization of an occluded cerebral artery may assist in the recovery of reversibly ischemic tissue and limit the neurological disability. In June 1996, the recombinant tissue plasminogen activator was approved as a safe and an effective intravenous treatment for acute ischemic stroke, especially if given within 3 …

Biomedical Research In The Kingdom Of Saudi Arabia (1982-2000), Ghazi O. Tadmouri, Nisrine B. Tadmouri

Saudi Medical Journal

No abstract provided.

Utilization Of Emergency Services In A Community Hospital, Saima Siddiqui, Danny O. Ogbeide

Saudi Medical Journal

OBJECTIVE: Over utilization of emergency room services by patients with non-urgent complaints is a global problem. It results in a waste of resources, stress among the emergency room staff and an increase in waiting time for patients requiring attention. This study was carried out to establish the extent of inappropriate emergency room attendance in a Saudi community. METHODS: Data was collected from the emergency room register, regarding the age, sex, presenting complaints, time of presentation and disposal of patients, from January 1st 1999 to March 31st 1999 at Al-Kharj Military Hospital, Al-Kharj, Kingdom of Saudi Arabia. Patients were classified in …

Caries Prevalence In Saudi Primary Schoolchildren Of Riyadh And Their Teachers' Oral Health Knowledge, Attitude And Practices, Amjad H. Wyne, Bandar M. Al-Ghorabi, Yahia A. Al-Asiri, Nazeer B. Khan

Saudi Medical Journal

OBJECTIVE: The purpose of the present study was to determine the caries prevalence and severity in primary school children Riyadh, Kingdom of Saudi Arabia, and to assess the oral health knowledge, attitude and practices of their teachers. METHODS: A random sample of primary schoolchildren was examined for dental caries utilizing World Health Organization criteria for diagnosis of caries. The information regarding oral health knowledge, attitude and practices in schoolteachers was collected through an especially designed self-administered questionnaire. RESULTS: The prevalence of caries among the sample was 94.4%. The mean decayed, missing and filled score for primary teeth of the sample …

Prevalence Of Systemic Lupus Erythematosus In Central Saudi Arabia, Abdurhman S. Al-Arfaj, Suliman R. Al-Balla, Abdullah N. Al-Dalaan, Salman S. Al-Saleh, Sultan A. Bahabri, Mohammed M. Mousa, Mohammed A. Sekeit

Saudi Medical Journal

OBJECTIVE: Although systemic lupus erythematosus of varying severity has been seen in our clinics, there is no information regarding the prevalence of the disease among Saudi nationals. We conducted this study to determine the prevalence of systemic lupus erythematosus in Al-Qaseem region of the Kingdom of Saudi Arabia. METHODS: A community survey of towns and villages in the Al-Qaseem area of central Saudi Arabia was conducted in 3 phases to determine the prevalence of systemic lupus erythematosus in the region. RESULTS: Of the 10,372 studied, 2 cases of systemic lupus erythematosus were identified using the criteria set for the diagnosis …

Patient Satisfaction Of The Obstetric And Gynecology Unit At King Khalid University Hospital, Riyadh, Kingdom Of Saudi Arabia, Rajakumari Ravi, Titilola O. Filani

Saudi Medical Journal

No abstract provided.

Etiology Of End-Stage Renal Disease In Najran, Kingdom Of Saudi Arabia, Latif A. Khan

Saudi Medical Journal

No abstract provided.

Going Beyond The Curriculum To Promote Medical Education And Practice., Mohamed A. Daw, Sherine Shawky

Saudi Medical Journal

No abstract provided.