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Gene Inhibition Using Genetic Suppressor Elements In Escherichia Coli Jm109de3 Cells, Ronald Chuke

Masters Theses & Specialist Projects

Genetic suppressor elements (GSEs) are short biologically active gene fragments that encode inhibitory antisense RNAs or truncated proteins that function as negative dominants. GSEs can be constructed from a single gene or isolated from a multigene library consisting of short random fragments of the target gene or genes. The goal of this study was to determine if gene inhibition in bacterial cells could be achieved by isolating GSEs from a genomic library. Additionally, if it was possible to use GSEs to inhibit gene function in E. coli JM109DE3 cells, could this method be used to locate and determine the function …

Density And Age Distributions Of Trees In Upland And Lowland Oldfields 60 Years After Abandonment: The Role Of Dispersal, Virginia Mcdaniel

Masters Theses & Specialist Projects

I attempted to determine whether the patterns of seedling establishment described in the literature for forests and recently abandoned oldfields are maintained in fields sixty years after abandonment. To determine this distribution pattern, as well as to compare patterns of age and density distribution in upland and lowland oldfields, I conducted research at Mammoth Cave National Park. I studied three species, the seeds of which are dispersed by three different modes: Juniperus virginiana, a predominantly bird-dispersed species; Acer rubrum, a predominantly winddispersed species; and Fagus grandifolia, a predominantly mammal-dispersed species. The establishment patterns observed by others in recently abandoned oldfields …

Psu/ Knea Communicator 05.00, Knea State Of Kansas

KNEA 50th Anniversary

This is a digital version of the May 2000 PSU/KNEA newsletter called the Communicator

Kentucky Warbler (Vol. 76, No. 2), Kentucky Library Research Collections

The Kentucky Warbler

No abstract provided.

Insights, Spring, 2000, Utah State University

Discovery Magazine

Bi-annual alumni newsletter for the College of Science of Utah State University in Logan.

University Of Minnesota, Morris 2000 Commencement, University Relations

UMM Commencement

The Thirty-seventh University of Minnesota, Morris Commencement Ceremonies at Four O'Clock Friday, the Twelfth of May, Two Thousand Morris, Minnesota

Effects Of Forced Weaning On Mother-Daughter Associations And Reproduction In Bison (Bos Bison), Jennifer S. Brookshier

Biology

While the social organization of ungulates has received much attention, the nature and extent of the mother-daughter association is still not fully known for some species. In bison, mothers and daughters have a long-term association that may extend beyond the age of weaning. This association is believed to facilitate the daughters' social integration into the herd. I compared the mother-daughter association for 29 yearling females that were forcibly weaned from their mothers to that of 19 yearling females that were naturally weaned. Forcibly weaned yearlings did not reassociate with their mothers following winter separation, while naturally weaned yearlings associated with …

Sexual Dichromatism In Bohemian Waxwings, James Brian Burns

Biology

If a species of birds is monomorphic, how do individuals identify members of the opposite sex? Birds may use cues in the ultraviolet wavelengths (350-400 nm), which are invisible to humans. This thesis presents evidence of sexual dichromatism in Bohemian Waxwings (Bombycilla garrulus). The strongest differences occurred in the tail coverts, the yellow tail tips, and the red waxy tips of the secondaries. Like other birds, waxwings derive colors from diet-based pigments and from synthesized colors. Because these color types differ in origin, each could provide different visual information about a bird's condition. This led to the testing …

Effects Of Carbohydrate Foods On Foraging Decisions Of The Parasitoid Bathyplectes Curculionis (Thomson) (Hymenoptera: Ichneumonidae), Helen Spafford Jacob

Biology

In a series of field and laboratory experiments, I explored the trade-off between self-maintenance and current reproduction for the parasitoid Bathyplectes curculionis (Thomson)(Hymenoptera: Ichneumonidae), an economically important natural enemy of the alfalfa weevil, the most significant pest in forage alfalfa. Floral nectars, pea aphid honeydew, and artificial foods such as sugar sprays are likely to assist B. curculionis in meeting its self-maintenance needs. Once these needs are met then B. curculionis females may be more likely to search for hosts to parasitize. However, these foods must not only be available, but accessible, of high quality, and detectable, to be of …

The Utilization Of Steep Slopes By Desert Tortoises (Gopherus Agassizii) In The Western Mojave Desert, Ty J. Gardner

Biology

Desert tortoises (Gopherus agassizii) were located on steep slopes at three sites in the western Mojave Desert. These sites were located at: 1) the rifle range of the Marine Corps Logistics Base, Barstow, California, 2) the southern boundary of the Fort Irwin National Training Center, California, immediately west of Manix Tank Trail, and 3) the Lava Range of the Marine Corps Air Ground Combat Center, Twentynine Palms, California. Additionally, a valley site at the Sand Hill range of the Marine Corps Air Ground Combat Center, Twentynine Palms, California, was included for comparison. Study tortoises were found on slopes …

Snfa, The Dictyostelium Discoideum Snf1/Ampk Protein Kinase, Sandy Sung

Biology

SNF1/AMPK protein kinases are a conserved group of eukaryotic serine/threonine protein kinases that mediate a variety of stress responses, including the response to nutrient deprivation. This report describes the isolation of snfA, the Dictyostelium discoideum SNF1/AMPK ortholog, and an examination of snfA protein function. snfA is a single copy gene that encodes a predicted 80.5 kD polypeptide. A single 2.8 kb snfA transcript was present at nearly constant levels during vegetative growth and all stages of development. The predicted snfA protein consists of three domains: an amino terminal kinase domain that is highly conserved in other SNF1/AMPK protein kinases, …

Contributions Of Domain Four Segment Four Charges To Sodium Channel Activity, Jennifer L. Abbruzzese

Biology

Ionic currents flowing through voltage-gated sodium (Na⁺) channels generate the rising phase of action potentials. This process is essential for normal cellular excitability and it is important to investigate the structure/function relationships that allow Na⁺ channels to change conformations in response to membrane potential. The Na⁺ channel skeletal muscle (SkM) α subunit is made of four homologous domains (DI-IV), with six transmembrane segments (S1-6) in each domain. One transmembrane segment in the channel, segment four in domain four (DIVS4), appears to make critical contributions to several aspects of channel activity. In this study, wild type (WT) …

Effect Of Daily Fluctuations From Flaming Gorge Dam On Ice Processes In The Green River, John W. Hayse, Steven F. Daly, Andrew Tuthill, Richard A. Valdez, Bryan Cowdell, Gary Burton

All U.S. Government Documents (Utah Regional Depository)

This report provides results and conclusions of a detailed investigation of ice processes in the main channel of the reach of the Green River between the downstream end of Split Mountain (River Mile [RM1] 320) and the Ouray, Utah Bridge (R.M248). The objective of the study was to examine the influence of daily fluctuations in water releases from Flaming Gorge Damon river ice processes in this reach, which serves as an overwintering area for endangered Colorado pikeminnow and razorback sucker. The objective of the study was met through examination of historical records of winter water and air temperatures, flow measurements, …

Fisheries Research Report No. 119 - Final Report, Frdc Project 95/037: The Biology And Stock Assessment Of The Tropical Sardine, Sardinella Lemuru, Off The Mid-West Coast Of Western Australia, Daniel J. Gaughan, Ronald W. Mitchell

Fisheries Research Reports

Exploratory fishing for the tropical sardine, Sardinella lemuru, offshore of Geraldton on the midwest coast of Western Australia in the early 1990s, led to the establishment of a developmental purse seine fishery in this region. This fishery showed potential for substantial expansion, but as there was no information on S. lemuru in WA there was a need to undertake research during the developmental period. The biology and fishery for S. lemuru in WA were therefore investigated over a three year period between July 1995 and June 1998 with the aim of providing stock assessment advice.

The biology of S. lemuru …

Fisheries Research Report No. 121 - Synopsis Of The Biology And Exploitation Of The Blue Swimmer Crab, Portunus Pelagicus Linnaeus, In Western Australia, Mervi I. Kangas

Fisheries Research Reports

This report summarises the existing studies which have provided information on the biology, population characteristics and exploitation of the blue swimmer crab, Portunus pelagicus, in Western Australia. Gaps in the existing knowledge are also highlighted to assist in targeting new research initiatives. Portunus pelagicus matures at about one year and in temperate regions females mate only once a year. This is because mature crabs moult only once a year. It appears from studies to date that Western Australian crabs are one genetic population. This is being further investigated. Migration of the blue swimmer crab occurs between estuaries and the ocean …

Information On Quota Management Of Rock Lobster Fisheries In South Australia, Tasmania And New Zealand., Kevin Donohue, Eric Barker

Fisheries Management Papers

This report provides an update on the information available to Western Australians on the quota management of Australasian rock lobster fisheries. The report keeps Government and industry abreast of developments in alternative management systems and, if necessary, reviews the WA management system.

La Esperanza, Elizabeth Tippet

Senior Scholar Papers

Poems

Information Interface - Volume 28, Issue 3 - May/June 2000, George Washington University, Himmelfarb Health Sciences Library

Information Interface (1976 - 2009)

News and information about Himmelfarb Health Sciences Library of interest to users.

Place, Disease And Mortality: Trimble County, Kentucky 1849-1894, Nancy Demaree

Masters Theses & Specialist Projects

This researcher describes the characteristics of place...physical, cultural and human...of a small Kentucky county and looks at the incidence of disease and dying that occurred in that place in the last half of the nineteenth century. The impact of death on particular subsets of the general population was given a closer evaluation. Very young, females and the slave/Black communities were investigated individually. The overall site and situation of all aspects of Trimble County, Kentucky were viewed in an effort to support the notion that it is the manner in which man interacts with this environment that causes disease and death …

The Effects Of Interviewer Self-Monitoring On Male Appearance Discrimination In Employment Decisions, Charles Mcdowell

Masters Theses & Specialist Projects

This research examined the effects of appearance discrimination toward men as regards employment decisions and the extent to which the degree of interviewer selfmonitoring influences these decisions. Past research has indicated that discrimination does indeed occur for women, but no empirical research has been conducted on male discrimination in which hair length has been manipulated. Specifically, length of hair (shoulder length, approximately 1 inch, and balding) was manipulated for potential job applicants. These conditions were examined across different types of jobs (traditionally conservative, neutral, and traditionally liberal). Several hypotheses are offered, with most focused on the concept that those scoring …

Thirty-Fourth Commencement 2000 (Undergraduate), Sacred Heart University

Commencement Programs

Program for the 2000 Undergraduate ceremony held May 21, 2000.

Outcrossing Rate And Inbreeding Depression In The Perennial Yellow Bush Lupine, Lupinus Arboreus (Fabaceae), Pamela Kittelson, John L. Maron

Biological Sciences Faculty Publications

Little is known about the breeding systems of perennial Lupinus species. We provide information about the breeding system of the perennial yellow bush lupine, Lupinus arboreus, specifically determining self-compatibility, outcrossing rate, and level of inbreeding depression. Flowers are self-compatible, but autonomous self-fertilization rarely occurs; thus selfed seed are a product of facilitated selfing. Based on four isozyme loci from 34 maternal progeny arrays of seeds we estimated an outcrossing rate of 0.78. However, when we accounted for differential maturation of selfed seeds, the outcrossing rate at fertilization was lower, ∼0.64. Fitness and inbreeding depression of 11 selfed and outcrossed families …

The Impacts Of A Nonindigenous Marine Predator In A California Bay, Edwin D. Grosholz, Gregory M. Ruiz, Cheryl A. Dean, Kim A. Shirley, John L. Maron, Peter G. Connors

Biological Sciences Faculty Publications

Coastal marine ecosystems worldwide are being altered rapidly by the invasion of nonindigenous species. Unlike terrestrial and freshwater systems, the impacts of an invading species have never been quantified on multiple trophic levels for a marine food web. We measured the impact of the nonindigenous green crab, Carcinus maenas, on a coastal marine food web in central California and found that this predator exerted strong “top-down” control, significantly reducing the abundances of several of the 20 invertebrate species monitored over a 9-yr period. Densities of native clams, Nutricola tantilla and Nutricola confusa, and native shore crabs, Hemigrapsus oregonensis, showed 5-fold …

Integrating Development With Evolution: A Case Study With Beetle Horns, Douglas J. Emlen

Biological Sciences Faculty Publications

No abstract provided.

Contraception And Abortion, Foes Or Friends?, Jacques Suaudeau

The Linacre Quarterly

No abstract provided.

Fish & Wildlife News: May/June 2000

Fish and Wildlife News

Contents:

Washington Office, Regional Reorganization 2
Rogers, Lambertson, Dwyer Retire 3
Salmon Hearings Stir NW Waters 7
Library Links to Biological Databases 9
New Millennium, New Service Video 10
Ranchers as Conservationists 13
Y2K Crisis Averted 14
Words and Images: A Special Section 17
A Volunteer with Passion 22
Finding Publications is Easy 25
Fish and Wildlife...In Brief 31
Moving Forward 32

Fisheries Research Report No. 120 - A 12-Month Survey Of Recreational Fishing In The Leschenault Estuary Of Western Australia During 1998, B E. Malseed, Neil R. Sumner, P C. Williamson

Fisheries Research Reports

A survey of recreational boat-based and shore-based fishing in the Leschenault Estuary was conducted between January 1998 and December 1998. The survey estimated the total annual boat-based recreational fishing effort as 33,392 fisher days, with 86% of this effort targeting blue swimmer crabs. The total annual shore-based recreational fishing effort was estimated to be 5,610 fisher days, with 88% of this effort targeting blue swimmer crabs.

The recreational blue swimmer crab catch from the Leschenault Estuary is substantial and exceeds the reported commercial catch. The estimated total recreational catch of blue swimmer crabs was 219,000 crabs or 45.7 tonnes. This …

Using The Spi To Identify Drought, C. T. Agnew

Drought Network News (1994–2001)

This article was written in response to the recent analysis of drought in Turkey by Komuscu (1999). The study showed the relationship between drought duration, drought frequency, and drought time scale using the Standardized Precipitation Index (SPI).

Utah State University Commencement, 2000 – Main Campus, Utah State University

Commencement Programs

107th Annual Commencement of Utah State University

The Effects Of Block Scheduling On Students With Special Needs, Kimberly C. Seifring

Theses and Dissertations

This study surveyed the professionals from two schools in the southern New Jersey area regarding their views of block scheduling as well as their views on the effectiveness of block scheduling among students with special needs. A total of 243 surveys were distributed and 61 were returned. The survey questioned the teachers in regards to the changes they had to incorporate into their daily instructional time upon switching to block scheduling, the advantages and disadvantages of block scheduling, and how to ensure that students with special needs experience success while participating in a block schedule. A majority of the participants …