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Articles 2101 - 2130 of 2291
Full-Text Articles in Entire DC Network
Using Tort Settlements To Cartelize, Ian Ayres
Using Tort Settlements To Cartelize, Ian Ayres
Valparaiso University Law Review
No abstract provided.
Fear Of Frankenfoods: A Better Labeling Standard For Genetically Modified Foods, Matthew Franken
Fear Of Frankenfoods: A Better Labeling Standard For Genetically Modified Foods, Matthew Franken
Minnesota Journal of Law, Science & Technology
No abstract provided.
Hydrolase And Oxido-Reductase Activities In Diuraphis Noxia And Rhopalosiphum Padi (Hemiptera: Aphididae), Xinzhi Ni, Sharron S. Quisenberry, Saowaluck Pornkulwat, James Lester Figarola, Steven R. Skoda, John E. Foster
Hydrolase And Oxido-Reductase Activities In Diuraphis Noxia And Rhopalosiphum Padi (Hemiptera: Aphididae), Xinzhi Ni, Sharron S. Quisenberry, Saowaluck Pornkulwat, James Lester Figarola, Steven R. Skoda, John E. Foster
Department of Entomology: Faculty Publications
Four hydrolases and five oxido-reductases were examined using native stacking polyacrylamide gel electrophoresis. Homogenate of Russian wheat aphid, Diuraphis noxia (Mordvilko), bird cherry-oat aphid, Rhopalosiphum padi (L.), "Arapahoe" (aphid-susceptible) and "Halt" (aphid-resistant) wheat, Triticum aestivum L., and powdery mildew-infected Erysiphe graminis DC. ex Merat f. sp. tritici Em. Marchal, Arapahoe wheat leaves were assayed for enzyme activities. Pectinesterase, polygalacturonase (or pectinase), cellulase, and amylase activities were examined in the hydrolase group. Catalase, peroxidase, catechol oxidase, superoxide dismutase, and ascorbate oxidase activities were examined in the group of oxido-reductases. The two aphid species had the same hydrolases but different oxido-reductases. Although …
Salinity And Shade Preferences Result In Ovipositional Differences Between Sympatric Tiger Beetle Species, W. Wyatt Hoback, Douglas A. Golick, Tina Marie Svatos, Stephen M. Spomer, Leon G. Higley
Salinity And Shade Preferences Result In Ovipositional Differences Between Sympatric Tiger Beetle Species, W. Wyatt Hoback, Douglas A. Golick, Tina Marie Svatos, Stephen M. Spomer, Leon G. Higley
Department of Entomology: Faculty Publications
1. Adult tiger beetles of the genus Cicindela often co-occur within a habitat but larvae do not. Larvae are sedentary and form usually permanent burrows at the site of oviposition where they require 1-3 years for development.
2. To test niche partitioning based on ovipositional preference, the behavior of two sympatric salt marsh tiger beetles, Cicindela circumpicta and C. togata (Coleoptera: Cicindelidae), were examined.
3. In laboratory studies, female C. circumpicta and C. togata distinguished between experimental salinities, with the former preferring 4 parts per thousand (ppt) and the latter preferring 12 ppt. In the field, C. circumpicta larvae were …
Prolonged Winter Undernutrition And The Interpretation Of Urinary Allantoin:Creatinine Ratios In White-Tailed Deer, Glenn D. Delgiudice, Ken D. Kerr, L. David Mech, Ulysses S. Seal
Prolonged Winter Undernutrition And The Interpretation Of Urinary Allantoin:Creatinine Ratios In White-Tailed Deer, Glenn D. Delgiudice, Ken D. Kerr, L. David Mech, Ulysses S. Seal
United States Geological Survey, Northern Prairie Wildlife Research Center: Publications
The urinary allantoin:creatinine (A:C) ratio (expressed in micromoles of allantoin to micromoles of creatinine) has shown potential as an index of recent winter energy intake in preliminary controlled studies of elk (Cervus elaphus) involving mild condition deterioration (up to 11% loss of body mass). To ensure reliable nutritional assessments of free-ranging cervids by measuring A:C ratios of urine in snow, it is essential to extend this work. We assessed the effect of moderate and severe winter nutritional restriction on urinary A:C ratios of captive white-tailed deer (Odocoileus virginianus) that lost up to 32% body mass and …
Temporal Flexibility Of Reproduction In Temperate-Breeding Dabbling Ducks, Gary Krapu
Temporal Flexibility Of Reproduction In Temperate-Breeding Dabbling Ducks, Gary Krapu
United States Geological Survey, Northern Prairie Wildlife Research Center: Publications
I compared nesting intervals during three consecutive years in five species of temperate-nesting dabbling ducks (Mallard [Anas platyrhynchos], Northern Pintail [Anas acuta], Northern Shoveler [Anas clypeata], Blue-winged Teal [Anas discors], Gadwall [Anas strepera]) and assessed whether differences existed in timing of refractoriness. Most nesting by females of all five species ended by the summer solstice. Nesting ended earliest for Northern Shovelers and Northern Pintails and latest for Gadwalls. Some Mallards, Blue-winged Teal, and Gadwalls continued to nest into mid- and late summer, whereas Northern Shovelers and Northern Pintails did not. …
Establishment Of Big Bluestem And Illinois Bundleflower Mixtures With Imazapic And Imazethapyr, Daniel D. Beran, Robert A. Masters, Roch E. Gaussoin, Fernando Rivas-Pantoja
Establishment Of Big Bluestem And Illinois Bundleflower Mixtures With Imazapic And Imazethapyr, Daniel D. Beran, Robert A. Masters, Roch E. Gaussoin, Fernando Rivas-Pantoja
Department of Agronomy and Horticulture: Faculty Publications
Weeds interfere with establishment of native grasses and legumes. A study was conducted to determine the influence of imazapic1 [(±)- 2-[4,5-dihydro-4-methyl-4-(1-methylethyl)-5-oxo-1H-imidazol-2-yl]- 5-methyl-3-pyridinecarboxylic acid] and imazethapyr [2-[4,5-dihydro- 4-methyl -4 - (1- methylethyl) -5 -oxo-1H- imidazol -2 - yl]5- ethyl -3- pyridinecarboxylic acid] on weed control and establishment of ‘Pawnee’ big bluestem (Andropogon gerardii Vitman var. gerardii Vitman) and Illinois bundleflower [Desmanthus illinoensis (Michx.) MacMill.] planted in three binary mixtures of 220:110, 165:165, and 110:220 pure live seed (PLS) m-2. Mixtures were seeded at cropland sites near Clay Center, Mead, and Lincoln, NE. Imazapic and imazeth- apyr …
Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass
Crop Quality And Utilization: A Twelve-Hour In Vitro Procedure For Sorghum Grain Feed Quality Assessment, Jeffrey F. Pedersen, Todd Milton, R. A. Mass
Department of Agronomy and Horticulture: Faculty Publications
Improvedmethods for assessing cereal crop feed value are a prerequisite for the genetic improvement of sorghum [Sorghum bicolor (L.) Moench] feed value. Rate of starch digestion is now commonly believed to be the limiting factor in sorghum utilization by cattle (Bos taurus). However, techniques to assess this trait are not useful to sorghum breeders because of high labor inputs, lab error associated with starch measurement, and need for high numbers of replications. The objective of this study was to develop a simple technique capable of identifying differences in digestion between sorghum and corn (Zea mays L.) …
Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins
Ethylene Production, Cluster Root Formation, And Localization Of Iron(Iii) Reducing Capacity In Fe Deficient Squash Roots, Brian M. Waters, Dale G. Blevins
Department of Agronomy and Horticulture: Faculty Publications
Dicots and non-graminaceous monocots have the ability to increase root iron(III) reducing capacity in response to iron (Fe) deficiency stress. In squash (Cucurbita pepo L.) seedlings, Fe(III) reducing capacity was quantified during early vegetative growth. When plants were grown in Fe-free solution, the Fe(III) reducing capacity was greatly elevated, reached peak activity on day 4, then declined through day 6. Root ethylene production exhibited a temporal pattern that closely matched that of Fe(III) reducing capacity through day 6. On the 7th day of Fe deficiency, cluster root morphology developed, which coincided with a sharp increase in the root Fe(III) …
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Department of Biochemistry: Faculty Publications
HilA activates the expression of Salmonella enterica serovar Typhimurium invasion genes. To learn more
about regulation of hilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In
addition to expected mutations, we identified Tn5 insertions in pstS, fadD, flhD, flhC, and fliA. Analysis of the
pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence
of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the
PhoR-PhoB two-component regulatory system, suggesting that hilA expression may …
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Multiple Factors Independently Regulate Hila And Invasion Gene Expression In Salmonella Enterica Serovar Typhimurium, Robin L. Lucas, C. Phoebe Lostroh, Concetta C. Dirusso, Michael P. Spector, Barry L. Wanner, Catherine A. Lee
Department of Biochemistry: Faculty Publications
HilA activates the expression of Salmonella enterica serovar Typhimurium invasion genes. To learn more
about regulation of hilA, we isolated Tn5 mutants exhibiting reduced hilA and/or invasion gene expression. In
addition to expected mutations, we identified Tn5 insertions in pstS, fadD, flhD, flhC, and fliA. Analysis of the
pstS mutant indicates that hilA and invasion genes are repressed by the response regulator PhoB in the absence
of the Pst high-affinity inorganic phosphate uptake system. This system is required for negative control of the
PhoR-PhoB two-component regulatory system, suggesting that hilA expression may …
Patterns Of Annual Seed Production By Northern Hemisphere Trees: A Global Perspective, Walter D. Koenig, Johannes M.H. Knops
Patterns Of Annual Seed Production By Northern Hemisphere Trees: A Global Perspective, Walter D. Koenig, Johannes M.H. Knops
School of Biological Sciences: Faculty Publications
We tested whether annual seed production (masting or mast fruiting) in Northern Hemisphere trees is an evolved strategy or a consequence of resource tracking by comparing masting patterns with those of annual rainfall and mean summer temperatures, two environmental variables likely to correlate with available resources. There were generally significant negative autocorrelations between the seed crop in year x and year x+1 (year x+2 in species of Quercus requiring 2 yr to mature acorns), as expected if resources are depleted in mast years in part by switching resources from growth to reproduction. Spatial autocorrelation in annual seed production generally declined …
Learning Improves Growth Rate In Grasshoppers, Reuven Dukas, Elizabeth A. Bernays
Learning Improves Growth Rate In Grasshoppers, Reuven Dukas, Elizabeth A. Bernays
School of Biological Sciences: Faculty Publications
To quantify the adaptive significance of insect learning, we documented the behavior and growth rate of grasshoppers (Schistocerca americana) in an environment containing two artificial food types, one providing a balanced diet of protein and carbohydrate, which maximizes growth, and the other being carbohydratedeficient, which is unsuitable for growth. Grasshoppers in the Learning treatment experienced a predictable environment, where the spatial location, taste, and color of each food source remained constant throughout the experiment. In contrast, grasshoppers of the Random treatment developed in a temporally varying environment, where the spatial location, taste, and color of the balanced and …
Role Of Esterase Gp70 And Its Influence On Growth And Development Of Dictyostelium Discoideum, Aidong Yuan, Catherine P. Chia
Role Of Esterase Gp70 And Its Influence On Growth And Development Of Dictyostelium Discoideum, Aidong Yuan, Catherine P. Chia
Papers in Microbiology
Gp70 is an esterase originally called crystal protein because of its presence in crystalline structures in aggregation-competent Dictyostelium discoideum cells. Although postulated to break down spore coats, the function of gp70 in vivo was incompletely investigated. Our immunolocalization and biochemical studies of vegetative D. discoideum amoebae show that gp70 was recruited to phagosomes and found in lysosomes. Purified gp70 was effective at hydrolyzing naphthyl substrates with acyl chains typical of lipids and lipopolysaccharides, indicating that the gp70 was involved in digesting endocytosed molecules. The activity of purified gp70 was inhibited by reductants that retarded its electrophoretic mobility and verified the …
A Cox2 Molecular Phylogeny Of The Peronosporomycetes, Deborah S.S. Hudspeth, Steven A. Nadler, Michael E.S. Hudspeth
A Cox2 Molecular Phylogeny Of The Peronosporomycetes, Deborah S.S. Hudspeth, Steven A. Nadler, Michael E.S. Hudspeth
Harold W. Manter Laboratory of Parasitology: Faculty Publications
The evolutionary history of the mitochondrial COX2 locus has been used to infer the phylogenetic relationships among 15 peronosporomycete and a hyphochytriomycete species. This molecular phylogenetic analysis at both the ordinal and generic levels provides strong evidence for the recognition of the Saprolegniomycetidae and the Peronosporomycetidae as natural groups, and for the monophyly of the Saprolegniales, Leptomitales and Pythiales. A three amino acid insertion/deletion event (indel) has been identified as a putative synapomorphy for the Saprolegniales. Parsimony mapping of 12 morphological and biochemical characters on the COX2 molecular phylogeny yields an hypothesis for peronosporomycete ancestral states and shared-derived features.
A Cultivated Taste For Yeast., C Brenner
A Cultivated Taste For Yeast., C Brenner
Kimmel Cancer Center Faculty Papers
The availability of complete genomic sequences of Saccharomyces cerevisiae has catalyzed a cultural change in the practice of yeast biology, providing opportunities to develop high throughput techniques to define protein function, to define drug targets, and to discover and characterize drugs.
Adolescent Endocrinology In Saudi Arabia, Mohamed A. Abdullah, Hamad S. Al-Salhi, Mona A. Anani, Liza Q. Melendrez
Adolescent Endocrinology In Saudi Arabia, Mohamed A. Abdullah, Hamad S. Al-Salhi, Mona A. Anani, Liza Q. Melendrez
Saudi Medical Journal
OBJECTIVE: The importance of providing special health care services for adolescents has been recently stressed. In Saudi Arabia, adolescents have no special services. In this study, we describe our first 5 years experience of an adolescent endocrinology clinic in Security Forces Hospital. We believe it is the first clinic with this objective in the Kingdom. METHODS: An adolescent endocrinology clinic was run by a team composed of pediatric endocrinologist, diabetic nurse educator, clinic nurse and a dietician in collaboration with other supportive staff and consultants from other disciplines. The methodology of setting up, staffing and running the clinic is described. …
Effect Of Chromium Supplementation On Glucose Tolerance And Lipid Profile, Suhad M. Bahijri
Effect Of Chromium Supplementation On Glucose Tolerance And Lipid Profile, Suhad M. Bahijri
Saudi Medical Journal
OBJECTIVE: To investigate chromium status of the adult population in the western region of Saudi Arabia and the possibility of using serum chromium status measurement as indicator of this status. METHODS: The effect of chromium supplement on glucose tolerance and lipid profile was studied in 44 normal, free living adults. 200mg chromium/day as CrCL3 or a placebo was given in a double blind cross-over study, with 8 weeks experimental periods. Fasting, 1 hour and 2 hour post glucose challenge (75g of glucose) glucose, serum fructosamine, total cholesterol, high-density lipoprotein-cholesterol, triglycerides, chromium and dietary intakes were estimated at the beginning and …
Substance Dependence. A Hospital Based Survey, Nayyer Iqbal
Substance Dependence. A Hospital Based Survey, Nayyer Iqbal
Saudi Medical Journal
OBJECTIVE: To collect basic data on multiple and diverse issues such as drugs of dependence, smoking, initiation age, injection related harm, nutritional status, trauma, accidents, abnormal laboratory parameters and co-morbid conditions. METHODS: Seven hundred and ninety nine subjects from a voluntary detoxification unit were studied. Four hundred and ninety seven charts from June 1995 to December 1995 were reviewed retrospectively. For the other 302 subjects admitted between September 1996 to December 1996, information regarding drug use, smoking, trauma, accidents and co-morbid conditions was recorded during the admission interview while the remaining data was later obtained from the charts. RESULTS: Sixty …
Prevalence Of Food Allergy In Asthmatic Patients, Bahaa A. Aba-Alkhail, Fathi M. El-Gamal
Prevalence Of Food Allergy In Asthmatic Patients, Bahaa A. Aba-Alkhail, Fathi M. El-Gamal
Saudi Medical Journal
OBJECTIVE: This study was conducted to assess the role of clinical sensitivity to food on the pattern of bronchial asthma. METHODS: A total of 1341 patients with asthma were included in the present study. The clinical sensitivity to food and its relation to respiratory symptoms were assessed cross-sectionally (using detailed questionnaires), and longitudinally during their regular visits to the asthma clinic using diet diary. Total IgE was determined for a subsample of the patients (No = 392). RESULTS: The prevalence of clinical sensitivity to food was 29%; about 2 thirds of the patients had high total IgE level. Asthmatic patients …
Hysterectomy - A Female Gynecologist's Perspective., A. Logambal, Lulu L. Al-Nuaim
Hysterectomy - A Female Gynecologist's Perspective., A. Logambal, Lulu L. Al-Nuaim
Saudi Medical Journal
No abstract provided.
Drug Addiction, Nasem A. Qureshi, Bahaa Abalkhail
Drug Addiction, Nasem A. Qureshi, Bahaa Abalkhail
Saudi Medical Journal
No abstract provided.
Micronutrient And Iron Supplementation And Effective Anti-Malarial Treatment Synergistically Improve Childhood Anaemia, H. Ekvall, Zul Premji, A. Bjorkman
Micronutrient And Iron Supplementation And Effective Anti-Malarial Treatment Synergistically Improve Childhood Anaemia, H. Ekvall, Zul Premji, A. Bjorkman
Pathology, East Africa
The control of childhood anaemia in malaria holoendemic areas is a major public health challenge for which an optimal strategy remains to be determined. Malaria prevention may compromise the development of partial immunity. Regular micronutrient supplementation has been suggested as an alternative but its effectiveness remains unsettled. We therefore conducted a randomised placebo-controlled intervention trial with 207 Tanzanian children aged 5 months to 3 years on the efficacy of supervised supplementation of low-dose micronutrients including iron (Poly Vi-Sol with iron) three times per week, with an average attendance of ≥ 90%. The mean haemoglobin (Hb) level increased by 8 g/l …
Plant Quality And Spider Predation Affects Grasshoppers (Acrididae): Food-Quality-Dependent Compensatory Mortality, Mark A. Oedekoven, Anthony Joern
Plant Quality And Spider Predation Affects Grasshoppers (Acrididae): Food-Quality-Dependent Compensatory Mortality, Mark A. Oedekoven, Anthony Joern
School of Biological Sciences: Faculty Publications
For insect herbivores, population responses reflect interactions among multiple factors, including abiotic conditions, food plant availability, and biotic interactions guided by food limitation and natural enemies. In this set of field experiments, we evaluated the interactive, dynamic effects of limited food supply and predation from wolf spiders (Lycosidae) on mortality in Ageneotettix deorum, a common grasshopper (Acrididae) from North American sandhills grasslands. We attempted to determine whether the combined effects of food availability and spider predation on mortality were (1) noncompensatory, with the effects of each factor summing to determine ultimate population size, or (2) compensatory, where the effect …
Biosystematics And Evolution Of The Triatominae = Biossistemática E Evolução De Triatomíneos, Christopher J. Schofield
Biosystematics And Evolution Of The Triatominae = Biossistemática E Evolução De Triatomíneos, Christopher J. Schofield
Harold W. Manter Laboratory of Parasitology: Library Materials
Abstract
In this paper we summarize the systematics of the 130 currently recognized species of Triatominae and the key features of their evolutionary background. There is increasing evidence that the subfamily has polyphyletic origins, with the various tribes and species groups probably arising from different reduviid lineages in relatively recent times.
Resumo
Neste trabalho resume-se a sistemática das 130 espécies de triatomíneos atualmente reconhecidas, com os elementos principais de sua evolução. Existem evidências crescentes para a origem polifilética da subfamília. As diferentes tribos e grupos de espécies que a compõem teriam surgido recentemente, a partir de diferentes linhagens de reduviídeos.
Coprolite Analysis: The Analysis Of Ancient Human Feces For Dietary Data, Karl Reinhard
Coprolite Analysis: The Analysis Of Ancient Human Feces For Dietary Data, Karl Reinhard
Karl Reinhard Publications
Although archaeological fieldwork is hot and dirty, the most “earthy” side of the discipline is the laboratory analysis of coprolites. Each coprolite contains the remains of one to several actual meals eaten in prehistory, and analysis of many coprolites provides a picture of ancient diet that is unique in accuracy.
The term coprolite originally referred to fossilized feces in paleontological context. In archaeology, the term broadened to refer to any formed fecal mass, including mineralized, desiccated, or frozen feces and even the intestinal contents of mummies. Coprolites contain the remains of animals (parasites) that lived in the humans, the foods …
Sheep-Predation Behaviors Of Wild-Caught, Confined Coyotes: Some Historical Data, Ray T. Sterner, Kenneth Crane
Sheep-Predation Behaviors Of Wild-Caught, Confined Coyotes: Some Historical Data, Ray T. Sterner, Kenneth Crane
United States Department of Agriculture Wildlife Services: Staff Publications
As part of efforts to develop The Livestock Protection Collar (U.S. EPA Reg. No. 56228-22), we videotaped sheep-predation events by 23 (15 males and 8 females) wild-caught, confined coyotes (Canis latrans) in a 31 x 41 m enclosure. Coyotes were paired individually with a sheep (Ovis aries) during 1 h daily trials. Nineteen (13 males and 6 females) of the coyotes made 75 fatal attacks of 1 to 7 sheep each; 4 coyotes (2 males and 2 females) made no fatal attacks despite 19 to 39 daily pairings. Of coyotes that made fatal attacks, 13 (9 …
Strychnine Alkaloid And Avian Reproduction: Effects Occur At Lower Dietary Concentrations With Mallard Ducks Than With Bobwhite Quail, C. A. Pedersen, R. T. Sterner, M. J. Goodall
Strychnine Alkaloid And Avian Reproduction: Effects Occur At Lower Dietary Concentrations With Mallard Ducks Than With Bobwhite Quail, C. A. Pedersen, R. T. Sterner, M. J. Goodall
United States Department of Agriculture Wildlife Services: Staff Publications
Separate subchronic reproductive toxicity studies were conducted using mallard ducks (Anas platyrhynchos) and northern bobwhite quail (Colinus virginianus). Three groups (32/group; 16 male-female pairs) of 17-week-old ducks (F0 generation) were fed Purina® Game Bird Breeder Layena® diets containing mean (±SD) 33.2 (±2.7), 68.9 (±1.8), and 140.9 (± 5.1) μg/g strychnine for 20 weeks, with some pairs in each group fed control diet during a subsequent 3-week recovery period. Three groups (32/group; 16 male-female pairs) of 19-week-old quail (F0 generation) were fed similar diets containing mean (±SD) 279.2 (±10.1), 557.4 (±43.5), and 1,113.6 (±46.6) μg/g …
Integrated Pest Management Of Black Bear Reforestation Damage, Gary W. Witmer, Dale L. Nolte, William Stewart
Integrated Pest Management Of Black Bear Reforestation Damage, Gary W. Witmer, Dale L. Nolte, William Stewart
United States Department of Agriculture Wildlife Services: Staff Publications
Black bear damage to commercial, coniferous trees on intensively managed public and private forest lands of the Pacific Northwest continues to be a problem for forest managers. Historically, methods such as relocation or spring hunts have been used in an effort to reduce bear density and damage. More recently, supplemental feeding has been used in an attempt to provide for the nutritional needs of bears during the damage period. Alternative silvicultural practices and repellents are being investigated for their ability to reduce the likelihood of bear damage. These and other methods need to be examined for their effectiveness, especially in …
Single-Dilution Enzyme-Linked Immunosorbent Assay For Quantification Of Antigen-Specific Salmonid Antibody, Stewart W. Alcorn, Ronald J. Pascho
Single-Dilution Enzyme-Linked Immunosorbent Assay For Quantification Of Antigen-Specific Salmonid Antibody, Stewart W. Alcorn, Ronald J. Pascho
United States Geological Survey: Staff Publications
An enzyme-linked immunosorbent assay (ELISA) was developed on the basis of testing a single dilution of serum to quantify the level of antibody to the p57 protein of Renibacterium salmoninarum in sockeye salmon (Oncorhynchus nerka). The levels of antibody were interpolated from a standard curve constructed by relating the optical densities (OD) produced by several dilutions of a high-titer rainbow trout (O. mykiss) antiserum to the p57 protein. The ELISA OD values produced by as many as 36 test sera on each microplate were compared with the standard curve to calculate the antigen-specific antibody activity. Repeated …