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1999

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Not-For-Profit Organizations With Conforming Changes As Of May 1, 1999; Audit And Accounting Guide:, American Institute Of Certified Public Accountants. Not-For-Profit Organizations Committee Jan 1999

Not-For-Profit Organizations With Conforming Changes As Of May 1, 1999; Audit And Accounting Guide:, American Institute Of Certified Public Accountants. Not-For-Profit Organizations Committee

Industry Guides (AAGs), Risk Alerts, and Checklists

No abstract provided.


Lady Griz Volleyball Media Guide, 1999, University Of Montana—Missoula. Athletics Department Jan 1999

Lady Griz Volleyball Media Guide, 1999, University Of Montana—Missoula. Athletics Department

Lady Griz Volleyball Media Guides, 1979-2008

Media guide created about the Lady Griz volleyball team.


Montana Business Quarterly, Winter 1999, University Of Montana--Missoula. Bureau Of Business And Economic Research Jan 1999

Montana Business Quarterly, Winter 1999, University Of Montana--Missoula. Bureau Of Business And Economic Research

Montana Business Quarterly, 1949-2021

This is an academic publication produced by the Bureau of Business and Economic Research (BBER) at the University of Montana’s College of Business. This is volume 37, number 4.


Illuminating The Possible In The Developing World: Guaranteeing The Human Right To Health In India, Sheetal B. Shah Jan 1999

Illuminating The Possible In The Developing World: Guaranteeing The Human Right To Health In India, Sheetal B. Shah

Vanderbilt Journal of Transnational Law

This Note argues that the recognition of the social right to health offers a step forward in empowering individuals to gain control over their social environments in the developing world. Part II discusses the potential of social human rights to alleviate suffering in the developing world. Social human rights recognize that the state must provide individuals with the basic social conditions necessary to live with human dignity. Part III explores the legal obligations of social rights and their current status in human rights jurisprudence. It also discusses the most pressing challenges facing implementation of social rights at the national level. …


Where's The Beef? Mad Cows And The Blight Of The Sps Agreement, Ryan D. Thomas Jan 1999

Where's The Beef? Mad Cows And The Blight Of The Sps Agreement, Ryan D. Thomas

Vanderbilt Journal of Transnational Law

This Note will first outline the SPS Agreement itself--specifically, Part II attempts to present the relevant articles in a manner providing the necessary background for understanding the WTO dispute panel and Appellate Body decisions. Next, Part III discuss and critique, the dispute panel and Appellate Body decisions, specifically, noting the shortcomings of these decisions in the context of the SPS Agreement and its utility as a precedent of international dispute resolution in the area of international regulation of drugs and feedstuffs. Next, I will addresses the likely effect of these decisions upon a possible WTO resolution of the SRM dispute …


The Sky Is Falling (Or Is It?): International Contracts And The Y2k Problem, Mark B. Baker Jan 1999

The Sky Is Falling (Or Is It?): International Contracts And The Y2k Problem, Mark B. Baker

Vanderbilt Journal of Transnational Law

Y2K problems at this point in time are reasonably foreseeable due to the amount of attention given the subject. Contracting parties should examine potential Y2K problems arising internally and address them before January 1, 2000. Yet the extent of Y2K problems, be they widespread or solitary occurrences, remains unforeseeable and unpredictable. Even those parties having adequately addressed internal Y2K problems can experience difficulties due to external parties having failed to become Y2K-compliant. This "second tier" of unforeseeability supports the use of excused performance, but the "first tier" foreseeability that Y2K problems potentially exist prevent viable use of the defense. In …


Psychoneuroimmunology And Healing Touch In Hiv Disease, Jo Lynne Wheeler Robins Jan 1999

Psychoneuroimmunology And Healing Touch In Hiv Disease, Jo Lynne Wheeler Robins

Theses and Dissertations

The purpose of this study was to ascertain the effects of Healing Touch (HT) on well-being and neuroendocrine function in individuals living with HIV disease. A total of 27 males completed the 4-week HT intervention. Each weekly HT session lasted 30 minutes and consisted of only the chakra connection, Because of the small sample size and the impact of gender on neuroendocrine and immune function, women were not included in this study Dependent variables included well-being as measured by three well-being and two psychological distress instruments, serum serotonin, salivary DHEA and cortisol, and a variety of enumerative and functional measures …


Reflections [Complete Issue : Fourth Quarter 1999, Vol. 25, 4], Sigma Theta Tau International, Diane Hamilton, Claudette Varricchio, Teresa B. Ades, Sue P. Heine, Heidi Darr-Hope, Carole D. Howell, Lillian Nail, Susan Larson Beck, Sally P. Weinrich, Maureen E. O'Rourke, Sigridur Halldorsdottir, Betty Rolling Ferrell Jan 1999

Reflections [Complete Issue : Fourth Quarter 1999, Vol. 25, 4], Sigma Theta Tau International, Diane Hamilton, Claudette Varricchio, Teresa B. Ades, Sue P. Heine, Heidi Darr-Hope, Carole D. Howell, Lillian Nail, Susan Larson Beck, Sally P. Weinrich, Maureen E. O'Rourke, Sigridur Halldorsdottir, Betty Rolling Ferrell

Sigma Past Publishing - Reflections Magazine

Publishing History: Print issues of Reflections magazine were published from 1975 to 1999 and its successor, Reflections on Nursing Leadership (RNL) began publication in 2000. RNL migrated to an online format, http://www.reflectionsonnursingleadership.org, in 2006 and continues today.

RNL is a member benefit of the Honor Society of Nursing, Sigma Theta Tau International (STTI). The historical print issues have been made openly available.

Publishing Frequency: Quarterly until its transition to online. It’s now updated virtually every day.

Format: Print, 1975 - 2005; Online, 2006 - present

Feature Articles in this Issue:

Caring at the End of Life

Points-of-no-return

Nursing's Holistic …


What Spending Clause? (Or The President's Paramour): An Examination Of The Views Of Hamilton, Madison, And Story On Article I, Section 8, Clause 1 Of The United States Constitution, Jeffrey T. Renz Jan 1999

What Spending Clause? (Or The President's Paramour): An Examination Of The Views Of Hamilton, Madison, And Story On Article I, Section 8, Clause 1 Of The United States Constitution, Jeffrey T. Renz

Faculty Law Review Articles

This article tests three interpretations of the General Welfare Clause that persisted prior to the U.S. v. Butler decision against the text of the Constitution and discusses historical conditions that add to the understanding of that clause of the U.S. Constitution. Specifically, the author examines: the "strong" Hamiltonian interpretation, the Madison interpretation, and the "weak" Hamiltonian or Story interpretation. The author concludes that in the course of testing each hypothesis, a surprising conclusion was reached: all interpretations failed to survive.


The West Digest System: The Ninth Circuit And The Montana Supreme Court, Fritz Snyder Jan 1999

The West Digest System: The Ninth Circuit And The Montana Supreme Court, Fritz Snyder

Faculty Law Review Articles

This article analyzes the West Digest System. The article first describes the West Digest System. Next, the article compares Montana jurisprudence with that of the Ninth Circuit in light of numerical analysis of the West Digest System. Then the article discusses certain digest topics as reflections of American law. Finally, the article examines the curious symbiotic relationship between the West Digest System and the growth of American jurisprudence.


Communication Of Social Support In Computer-Mediated Groups For People With Disabilities, Dawn O. Braithwaite, Vincent R. Waldron, Jerry Finn Jan 1999

Communication Of Social Support In Computer-Mediated Groups For People With Disabilities, Dawn O. Braithwaite, Vincent R. Waldron, Jerry Finn

Department of Communication Studies: Faculty Publications

This study documented the types and extent of social support messages exchanged by persons with disabilities who participated in a computer-based support group. A modified version of Cutrona and Suhr’s (1992) social support category system was used to code 1,472 support messages. The largest percentage of these messages offered emotional and informational support, whereas network support and tangible assistance were least frequently offered. It appeared that many of the support messages directly redressed limitations and challenges associated with disability-related mobility, socialization, and self-care. Results are discussed in terms of the generalizability of existing category systems for coding support to this …


Protecting Communication Departments: Reflections On The Nebraska Experience, Ronald Lee, William J. Seiler Jan 1999

Protecting Communication Departments: Reflections On The Nebraska Experience, Ronald Lee, William J. Seiler

Department of Communication Studies: Faculty Publications

Eight years ago, in the first week of the 1991 fall semester, the Acting Senior Vice Chancellor for Academic Affairs announced a series of vertical budget cuts that included the elimination of the University of Nebraska–Lincoln’s department of Speech Communication (now Communication Studies). Over the next seven months the department fought against the proposed action. In March 1992, the Budget Reduction Review Committee voted against the Vice Chancellor’s recommendation. Later in the month, the Academic Planning Committee also voted to rescind the budget cutting measure.

These actions ended the battle and assured the continuation of the department. In an earlier …


The Times, They Are A Changing: A Review Of Raising Children In A Socially Toxic Environment, Susan M. Sheridan, Richard J. Cowan, Tanya Meegan Jan 1999

The Times, They Are A Changing: A Review Of Raising Children In A Socially Toxic Environment, Susan M. Sheridan, Richard J. Cowan, Tanya Meegan

Department of Educational Psychology: Faculty Publications

Rarely does a book come along that we believe to be essential reading for all psychologists, educators, child care workers, physicians, clergy, and concerned community members. Garbarino’s Raising Children in a Socially Toxic Environment is one such book. In this book, Garbarino sets out to offer his views on what childhood ought to be, how children map their own worlds (thus define, themselves), what their basic needs are, the levels in which those needs are being met, as well as offer suggestions for ways in which readers can change the “toxic environment” to aid in the healthy development of children …


Alcohol Use Among A Sample Of Thai Adolescents, Ian Newman, Saranya Innadda Jan 1999

Alcohol Use Among A Sample Of Thai Adolescents, Ian Newman, Saranya Innadda

Department of Educational Psychology: Faculty Publications

Beverage alcohol use is common in almost all societies and has been for thousands of years. Societies that use alcohol have rituals and traditions that tend to regulate alcohol's negative effects and enhance its pleasurable effects. Modern alcohol production and marketing methods have tended to break down these traditional controls and encourage irresponsible use. In many western countries, irresponsible use of alcohol is associated with crime, violence, and disease. In particular, for adolescents, alcohol use is linked to automobile crashes, injuries, and deaths. Alcohol use is associated with so many deaths and injuries that most western countries now consider alcohol …


Using Responsive Evaluation To Evaluate A Professional Conference, Amy N. Spiegel, Roger H. Bruning, Lisa Giddings Jan 1999

Using Responsive Evaluation To Evaluate A Professional Conference, Amy N. Spiegel, Roger H. Bruning, Lisa Giddings

Department of Educational Psychology: Faculty Publications

In a statewide conference on alternative methods for assessing students’ learning, we incorporated responsive evaluation methods into the structure of the conference. The application of these interactive evaluation techniques serves as a pilot study that illustrates the possible utility of these tech-niques in evaluating conferences. This paper provides a brief review of the literature surrounding responsive evaluation, a description of the responsive evaluation methods applied to this conference, and a discussion of the results and implications of this pilot study.


Influence Of Agriculture On Aquatic Invertebrate Communities Of Temporary Wetlands In The Prairie Pothole Region Of North Dakota, Usa, Ned H. Euliss Jr., David M. Mushet Jan 1999

Influence Of Agriculture On Aquatic Invertebrate Communities Of Temporary Wetlands In The Prairie Pothole Region Of North Dakota, Usa, Ned H. Euliss Jr., David M. Mushet

United States Geological Survey, Northern Prairie Wildlife Research Center: Publications

We evaluated the influence of intensive agriculture on invertebrate communities of temporary wetlands as indicated by aquatic invertebrate resting eggs, shells, and cases remaining after wetlands dried. To facilitate the comparison, we sampled 19 wetlands within cropland areas and 19 wetlands within grassland areas. We found resting eggs, shells, and cases of significantly more taxa and greater numbers of cladoceran resting eggs (ephippia), planorbid and physid snail shells, and ostracod shells in wetlands within grasslands than in croplands. We also successfully incubated greater numbers of cladocerans and ostracods from soil samples collected from grassland sites. We were unable to detect …


Final General Management Plan Environmental Impact Statement Jan 1999

Final General Management Plan Environmental Impact Statement

United States Army Corps of Engineers, Omaha District

This Final General Management Plan / Environmental Impact Statement presents three alternatives for the future of the Missouri National Recreational River: a continuation of existing (conditions (no-action) alternative, a resource protection/recreation (preferred) alternative emphasizing protection and enhancement of biologic values and the history and culture of the area, and a recreational emphasis alternative. In both action alternatives, the Corps of Engineers (COE) and the National Park Service (NPS) would manage the area through a cooperative agreement. The Corps of Engineers would function as the day-la-day manager of the water-related resources, while the National Park Service would administer the land-related resources. …


Changes In Early Winter Abundance Of Four Gull (Larus) Species On Western Lake Erie, 1951-1995, Laura A. Tyson, Richard A. Dolbeer, Jerrod L. Belant Jan 1999

Changes In Early Winter Abundance Of Four Gull (Larus) Species On Western Lake Erie, 1951-1995, Laura A. Tyson, Richard A. Dolbeer, Jerrod L. Belant

United States Department of Agriculture Wildlife Services: Staff Publications

Many gulls (Larus spp.) that nest on and north of the Great Lakes concentrate along the south shore of western Lake Erie in early winter. Monitoring population trends of these gulls is important because of increasing conflicts between gulls and humans. We used data from four Christmas Bird Count locations to examine population trends of four gull species on western Lake Erie during nine 5-year periods, 1951-1995. Overall, ring-billed gulls (Z. delawarensis} were the dominant species (59% of gulls counted) followed by herring (Z. argentatus, 20%), Bonaparte's (Z. Philadelphia, 18%) and great blackbacked (Z. marinus, 0.2%) gulls. Ring-billed gulls displayed …


Plasmd Bearing A Cdna Copy Of The Genome Of Bovine Viral Diarrhea Virus, Chimeric Derivatives Thereof, And Method Of Producing An Infectious Bovine Wral Darrheavirus Using Sad Plasmid, Ruben O. Donis, Ventzislav B. Vassilev Jan 1999

Plasmd Bearing A Cdna Copy Of The Genome Of Bovine Viral Diarrhea Virus, Chimeric Derivatives Thereof, And Method Of Producing An Infectious Bovine Wral Darrheavirus Using Sad Plasmid, Ruben O. Donis, Ventzislav B. Vassilev

School of Veterinary and Biomedical Sciences: Faculty Publications

A plasmid bearing a cDNA copy of the genome of bovine viral diarrhea virus (BVDV), chimeric derivatives of the plasmid and a method of producing an infectious bovine viral diarrhea virus using the plasmid are disclosed. The invention relates to a plasmid DNA molecule that replicates easily in E. coli and contains a sufficient portion of the genome of BVDV, cloned as cDNA, to be a suitable template to produce RNA in vitro which, upon transfection into bovine cells, gives rise to infectious BVDV. The BVDV created by the process of the invention can be engineered for use as a …


Conjunctivitis Caused By A Swine Chlamydia Trachomatis-Like Organism In Gnotobiotic Pigs, Douglas G. Rogers, Arthur A. Andersen Jan 1999

Conjunctivitis Caused By A Swine Chlamydia Trachomatis-Like Organism In Gnotobiotic Pigs, Douglas G. Rogers, Arthur A. Andersen

School of Veterinary and Biomedical Sciences: Faculty Publications

The objective of this study was to determine whether a chlamydial strain recovered from growing and finishing swine with conjunctivitis or keratoconjunctivitis could cause the same infections in gnotobiotic pigs. The strain shares biological characteristics with Chlamydia trachomatis. After propagation in Vero cells and preparation of the inoculum (107 inclusion-forming units/ml), chlamydial strain H7 was instilled into the ventral conjunctival sac (0.15 ml/sac) of 12 anesthetized 3-day-old gnotobiotic piglets. Four age-matched gnotobiotic piglets were anesthetized and sham infected with uninfected cell culture lysates. None of the principal piglets developed clinical symptoms of conjunctivitis or keratoconjunctivitis. Principal piglets necropsied 7 …


In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson Jan 1999

In Situ Hybridization For The Detection And Localization Of Swine Chlamydia Trachomatis, C. Chae, D.-S. Cheon, D. Kwon, O. Kim, B. Kim, J. Suh, D. G. Rogers, K. D. E. Everett, A. A. Anderson

School of Veterinary and Biomedical Sciences: Faculty Publications

Gnotobiotic piglets were inoculated intralaryngeally with swine Chlamydia trachomatis strain R33 or orally with swine C. trachmatis strain R27. Archived formalin-fixed, paraffin-embedded tissues from piglets euthanatized 4–7 days postinoculation were examined by in situ hybridization for C. trachomatis nucleic acid using a nonradioactive digoxigenin-labeled DNA probes that targeted specific ribosomal RNA or omp1 mRNA molecules of the swine C. trachomatis strains. Positive hybridization signals were detected in bronchial epithelial cells, bronchiolar epithelial cells, pneumocytes, alveolar and interstitial macrophages, and jejunal and ileal enterocytes. Chlamydia-infected cells had a strong signal that was confined to the intracytoplasmic inclusions. Positive hybridization signals were …


Quantitation Of Pyrethrum Residues In Brown Tree Snakes, John J. Johnston, Carol A. Furcolow, Stephanie A. Volz, Richard E. Mauldin, Thomas M. Primus, Peter J. Savarie, Joe E. Brooks Jan 1999

Quantitation Of Pyrethrum Residues In Brown Tree Snakes, John J. Johnston, Carol A. Furcolow, Stephanie A. Volz, Richard E. Mauldin, Thomas M. Primus, Peter J. Savarie, Joe E. Brooks

United States Department of Agriculture Wildlife Services: Staff Publications

A reversed-phase solid-phase extraction–gas chromatography (SPE–GC)–electron capture detection method is developed to quantitate individual rethrin residues in pyrethrum-exposed brown tree snakes. Aliquots (6 g) of homogenized snake tissue are extracted with 10 mL acetonitrile. The rethrins are recovered from the acetonitrile extract and concentrated using C8 SPE. The rethrins are eluted from the SPE column with pentane, evaporated to near dryness, and reconstituted to 1 mL with 1-propanol. Individual rethrins are quantitated using GC analysis of the 1-propanol solution. Method limits of detection for rethrins range from 0.63 to 6.51 ng/g. The mean recovery for all rethrins is 70.8% …


A Review Of Issues Pertaining To Transgenic Turfgrasses, Paul G. Johnson, Terrance P. Riordan Jan 1999

A Review Of Issues Pertaining To Transgenic Turfgrasses, Paul G. Johnson, Terrance P. Riordan

Department of Agronomy and Horticulture: Faculty Publications

Recombinant DNA technology is a rapidly growing field in plant and animal breeding. This technology involves the transfer of pieces of DNA, or genes, regardless of the organisms involved or how they are related. Transformation procedures in plants were first developed for important crop and model plant systems. Although turfgrass management and production is one of the fastest growing areas of agriculture, genetic transformation of turfgrasses lags behind that of many other important crop plants. Turfgrasses are becoming more important primarily because of their association with the ever-increasing urban population. The size of the turfgrass seed market is second only …


The Certified Safe Farm Project In Nebraska: The First Year, Jerry Jaspersen, Susanna Von Essen, Pam List, Larry Howard, David Morgan Jan 1999

The Certified Safe Farm Project In Nebraska: The First Year, Jerry Jaspersen, Susanna Von Essen, Pam List, Larry Howard, David Morgan

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Potentially preventable death, disabling injury, and serious illness are very common secondary to work in production agriculture in Nebraska and elsewhere. The traditional approach to farm safety has consisted largely of education designed to prevent traumatic injury. While education is of critical importance, additional incentives may be needed to reduce fatal and non-fatal events to an acceptable level. The Certified Safe Farm concept was developed in collaboration with two Nebraska health insurance companies. The program consists of combining an on-farm safety assessment and occupational health screening with an education program. The incentive to complete this program is to be eligible …


Seepage Characteristics And Hydraulic Properties Of A Feedlot Runoff Storage Pond, David B. Parker, Dean E. Eisenhauer, Dennis D. Schulte, John A. Nienaber Jan 1999

Seepage Characteristics And Hydraulic Properties Of A Feedlot Runoff Storage Pond, David B. Parker, Dean E. Eisenhauer, Dennis D. Schulte, John A. Nienaber

Department of Agricultural and Biological Systems Engineering: Faculty Publications

Water and chemical transport were investigated beneath a 22-year-old beef feedlot runoff storage pond. Soil and sludge samples were collected from 14 borings to 6.1-m depths in a cross-section across the pond. The soils consisted of silt loam and clay loam, and the groundwater level was about 30 m beneath the land surface. Soil samples were analyzed for pH, NO3-N, NH4-N, Cl, P, K, SO4-S, TKN, and organic matter. Physical and hydraulic properties were measured on undisturbed samples of soil and sludge to compare saturated hydraulic conductivity, moisture release characteristics, and bulk density. Saturated …


A Method For Estimating Marine Habitat Values Based On Fish Guilds, With Comparisons Between Sites In The Southern California Bight, Alan B. Bond, John S. Stephens, Jr., Daniel J. Pondella, Ii, M. James Allen, Mark Helvey Jan 1999

A Method For Estimating Marine Habitat Values Based On Fish Guilds, With Comparisons Between Sites In The Southern California Bight, Alan B. Bond, John S. Stephens, Jr., Daniel J. Pondella, Ii, M. James Allen, Mark Helvey

Papers in Behavior in Biological Sciences

Habitat valuation is an essential tool for tracking changes in habitat quality and in adjudicating environmental mitigation. All current methods for estimating habitat values of coastal marine sites rely heavily on the opinion of experts or on data variables that can readily be manipulated to influence the outcome. As a result, unbiased, quantitative comparisons between the values of different marine habitats are generally unavailable. We report here on a robust, objective technique for the valuation of marine habitats that makes use of data that are commonly gathered in surveys of marine fish populations: density, fidelity, and mean size. To insure …


Early And Late Endosomal Compartments Of Entamoeba Histolytica Are Enriched In Cysteine Proteases, Acid Phosphatase And Several Ras-Related Rab Gtpases, Lesly A. Temesvari, Ed Harris, Samuel L. Stanley Jr., James Cardelli Jan 1999

Early And Late Endosomal Compartments Of Entamoeba Histolytica Are Enriched In Cysteine Proteases, Acid Phosphatase And Several Ras-Related Rab Gtpases, Lesly A. Temesvari, Ed Harris, Samuel L. Stanley Jr., James Cardelli

Department of Biochemistry: Faculty Publications

Pure populations of early and late endosomes of Entamoeba histolytica were isolated by magnetic fractionation and characterized. It was shown that these vesicles were enriched in acid phosphatase and cysteine protease activities. An important virulence factor, a 27-kDa cysteine protease, was also enriched in early and late endosomes of E. histolytica. These data suggest that E. histolytica hydrolases reside in compartments that are part of or communicate with the endosomal pathway. To begin to identify the role of Rab GTPases in E. histolytica, an oligonucleotide approach was employed to screen an E. histolytica cDNA library for genes encoding …


Bacterial Growth State Distinguished By Single-Cell Protein Profiling: Does Chlorination Kill Coliforms In Municipal Effluent?, David Rockabrand, Teresa Austin, Robyn Kaiser, Paul H. Blum Jan 1999

Bacterial Growth State Distinguished By Single-Cell Protein Profiling: Does Chlorination Kill Coliforms In Municipal Effluent?, David Rockabrand, Teresa Austin, Robyn Kaiser, Paul H. Blum

School of Biological Sciences: Faculty Publications

Municipal effluent is the largest reservoir of human enteric bacteria. Its public health significance, however,

depends upon the physiological status of the wastewater bacterial community. A novel immunofluorescence

assay was developed and used to examine the bacterial growth state during wastewater disinfection. Quantitative

levels of three highly conserved cytosolic proteins (DnaK, Dps, and Fis) were determined by using

enterobacterium-specific antibody fluorochrome-coupled probes. Enterobacterial Fis homologs were abundant

in growing cells and nearly undetectable in stationary-phase cells. In contrast, enterobacterial Dps homologs

were abundant in stationary-phase cells but virtually undetectable in growing cells. The range of variation in

the abundance of …


Chlorella Virus Pbcv-1 Encodes A Functional Homospermidine Synthase, Annette Kaiser, Michael Vollmert, Dorothea Tholl, Michael V. Graves, James Gurnon, Weimei Xing, Amber D. Lisec, Kenneth Nickerson, James L. Van Etten Jan 1999

Chlorella Virus Pbcv-1 Encodes A Functional Homospermidine Synthase, Annette Kaiser, Michael Vollmert, Dorothea Tholl, Michael V. Graves, James Gurnon, Weimei Xing, Amber D. Lisec, Kenneth Nickerson, James L. Van Etten

Kenneth Nickerson Papers

Sequence analysis of the 330-kb genome of chlorella virus Paramecium bursaria chlorella virus 1 (PBCV-1) revealed an open reading frame, A237R, that encodes a protein with 34% amino acid identity to homospermidine synthase from Rhodopseudomonas viridis. Expression of the a237r gene product in Escherichia coli established that the recombinant enzyme catalyzes the NAD+-dependent formation of homospermidine from two molecules of putrescine. The a237r gene is expressed late in PBCV-1 infection. Both uninfected and PBCV-1-infected chlorella, as well as PBCV-1 virions, contain homospermidine, along with the more common polyamines putrescine, spermidine, and cadaverine. The total number of polyamine …


Absence Of Tuberculosis In Free-Ranging Deer In Nebraska, David J. Steffen, David W. Oates, Mauritz C. Sterner, Vickie L. Cooper Jan 1999

Absence Of Tuberculosis In Free-Ranging Deer In Nebraska, David J. Steffen, David W. Oates, Mauritz C. Sterner, Vickie L. Cooper

Harold W. Manter Laboratory of Parasitology: Faculty Publications

Lymph nodes from 271 white-tailed deer (Odocoileus virginianus) and mule deer (Odocoileus hemionus) in Nebraska (USA) were examined microscopically for tuberculoid lesions. Lymph nodes lesions in at least one node were found in 12 deer. Lesions were examined with Zeihl-Neelson acid fast stains and by polymerase chain reactions using M. bovis specific probes. No evidence of tuberculosis was found. The small granulomatous lesions were likely caused by other bacteria.