Digital Commons Network^™

Chemical Repellents, Scott J. Werner, Michael L. Avery

United States Department of Agriculture Wildlife Services: Staff Publications

Agricultural depredations caused by blackbirds can be managed with various lethal and nonlethal methods, including chemical repellents. For many people, nonlethal chemical repellents represent an appealing approach to managing crop depredation because the depredating birds are targeted but not killed; they are just inconvenienced. An effective repellent application can cause the crop-depredating birds to leave their present feeding site and seek food elsewhere. Where the birds go to feed is immaterial to the producer as long as the birds leave the producer's field. Thus, an effective repellent application will not likely affect the overall size of the blackbird population, but …

Ontogenetic And Ecological Variation In Invasion Risk Of Brown Treesnakes (Boiga Irregularis) On Guam, Shane R. Siers, Julie A. Savidge, Robert N. Reed

United States Department of Agriculture Wildlife Services: Staff Publications

Size structure within populations of invasive species may have consequences for relative risk at all stages of the invasion process, with implications for management interventions such as interdiction, suppression, and eradication. To assess relative distributions of invasive Brown Treesnakes (Boiga irregularis) among demographic categories of management interest, we undertook the most comprehensive and controlled sampling in > 25 years of research into this ecologically and economically destructive introduced predator. We collected a seasonally-balanced sample of 100 snakes from each of 18 sites, stratified by six habitat types, encompassing the species’ entire extralimital range. Samples indicated significant differences in distributions …

History Of Regulations, Policy, And Research Related To Conflicts Between Blackbirds And People, George M. Linz, Michael L. Avery, Richard A. Dolbeer

United States Department of Agriculture Wildlife Services: Staff Publications

The United States and Canada have invested substantial resources over the past 60 years for developing methods to reduce blackbird (Icteridae) damage to agricultural crops, to manage large winter roosts that create nuisance and public health problems, and to mitigate conflicts with endangered species. It is an indication of the challenging nature of the conflicts with these abundant, highly mobile birds that we are still attempting to improve existing methods and develop new approaches to mitigate the problems. Scientists have tested chemical frightening agents and repellents, mechanical scare devices, bird-resistant sunflowers, decoy crops, habitat management, population management, and cultural modifications …

Study Design Concepts For Inferring Functional Roles Of Mammalian Top Predators, Richard M. Engeman, Lee R. Allen, Benjamin L. Allen

United States Department of Agriculture Wildlife Services: Staff Publications

The roles and functions of top predators have in recent years been an important yet controversial field of biodiversity conservation research. Interrelationships between sympatric species within complex systems can pose enormous challenges for designing studies that gain clear understanding of specific relationships and processes. Teasing out the nature of the relationships is made far more difficult, if not impossible, if the experimental design of the studies is flawed or too limited for the desired inferences, and/or if the observational methods are inappropriate or too unwieldy to obtain the necessary data validly. The most powerful observation methods for understanding the interrelationships …

Wetland Bird Abundance And Safety Implications For Military Aircraft Operations, Kent Andersson, Craig A. Davis, Bradley F. Blackwell, Jason R. Heinen

United States Department of Agriculture Wildlife Services: Staff Publications

Wetlands with associated avifauna can pose a substantial hazard to aviation safety, potentially increasing bird–aircraft collision (strike) risk when located near air operations areas.We modeled year-round use by wetland avifauna of Drummond Flats Wildlife Management Area (Drummond Flats), a wetland complex located within 10 km of Vance Air Force Base (AFB), Enid, Oklahoma, USA. Our objectives were to 1) quantify seasonal avifauna abundances at Drummond Flats; 2) test a priori models reflecting use by bird species recognized as hazardous to aviation safety relative to environmental factors including flooded wetland habitat and vegetation cover; 3) use these models to predict maximal …

Can We Save Large Carnivores Without Losing Large Carnivore Science?, Benjamin L. Allen, Lee R. Allen, Henrik Andrén, Guy Ballard, Luigi Boitani, Richard M. Engeman, Peter J. S. Fleming, Adam T. Ford, Peter M. Haswell, Rafal Kowalczyk, John D. C. Linnell, L. David Mech, Daniel M. Parker

United States Department of Agriculture Wildlife Services: Staff Publications

Large carnivores are depicted to shape entire ecosystems through top-down processes. Studies describing these processes are often used to support interventionist wildlife management practices, including carnivore reintroduction or lethal control programs. Unfortunately, there is an increasing tendency to ignore, disregard or devalue fundamental principles of the scientific method when communicating the reliability of current evidence for the ecological roles that large carnivores may play, eroding public confidence in large carnivore science and scientists. Here, we discuss six interrelated issues that currently undermine the reliability of the available literature on the ecological roles of large carnivores: (1) the overall paucity of …

Determination Of 20, 25-Diazacholesterol In Avian Matrices By High Performance Liquid Chromatography/Tandem Mass Spectrometry, Benjamin G. Abbo, Laura E. Hulslander, David A. Goldade

United States Department of Agriculture Wildlife Services: Staff Publications

Wildlife contraceptives are an emerging tool for minimizing human-wildlife conflicts. One promising avian contraceptive compound, 20,25-diazacholesterol (DAC), reduces fertility by inhibiting cholesterol synthesis. A reliable analytical method for DAC was required in support of its registration for use as a reproductive control agent in pest bird species. A liquid chromatographic method employing tandem mass spectrometry (LC–MS/MS) was developed for the analysis of tissue extracts following solid phase extraction clean-up. Tissues analyzed were whole body samples from crows, monk parakeets, and quails and liver samples from crows and quails. Excellent sensitivity and selectivity was afforded by tandem mass spectrometry. The method …

Toxic Effects Of Orally Ingested Oil From The Deepwater Horizon Spill On Laughing Gulls, Katherine E. Horak, Steven J. Bursian, C. K. Ellis, K. M. Dean, J. E. Link, Katie C. Hanson-Dorr, F. L. Cunningham, K. E. Harr, C. A. Pritsos, Karen L. Pritsos, Katherine A. Healy, Dave Cacela, S. A. Shriner

United States Department of Agriculture Wildlife Services: Staff Publications

The explosion of the Deepwater Horizon oil rig released millions of gallons of oil into the environment, subsequently exposing wildlife, including numerous bird species. To determine the effects of MC252 oil to species relevant to the Gulf of Mexico, studies were done examining multiple exposure scenarios and doses. In this study, laughing gulls (Leucophaeus atricilla, LAGU) were offered fish injected with MC252 oil at target doses of 5 or 10 mL/kg bw per day. Dosing continued for 27 days. Of the adult, mixed-sex LAGUs used in the present study, 10 of 20 oil exposed LAGUs survived to the …

Space Use And Social Ecology Of Coyotes (Canis Latrans) In A High-Elevation Ecosystem: Relative Stability In A Changing Environment, Suzanne J. Gifford, Eric M. Gese, Robert R. Parmenter

United States Department of Agriculture Wildlife Services: Staff Publications

Coyote (Canis latrans) spatial and social ecology are variable, but have been little studied in high-elevation environments. In these temperate ecosystems, large ungulates are prevalent and coyote pack size may be large in order for them to scavenge and defend ungulate carcasses from conspecifics in neighboring packs. We initiated a study to understand the spatial and social ecology of coyotes on the Valles Caldera National Preserve, a high-elevation (2450–3400 m) protected area in northern New Mexico. Our objectives were to (1) describe the home range size and habitat use of coyotes in the preserve, (2) describe coyote movements …

Using Pentosidine And Hydroxyproline To Predict Age And Sex In An Avian Species, Brian S. Dorr, Randal S. Stahl, Katie C. Hanson-Dorr, Carol A. Furcolow

United States Department of Agriculture Wildlife Services: Staff Publications

All living organisms are subject to senescence accompanied by progressive and irreversible physiological changes. The error damage and cross-linking theories suggest that cells and tissues are damaged by an accumulation of cross-linked proteins, slowing down bodily processes and resulting in aging. A major category of these cross-linked proteins are compounds called advanced glycation end products (AGEs). We investigated the relationship between accumulation of the AGE, pentosidine (Ps), and hydroxyproline (HYP) a post-translationally modified amino acid, with age, sex, and breeding status (breeder/ nonbreeder) from skin samples of known age (i.e., banded as fledglings), free-ranging Double-crested Cormorants (Phalacrocorax auritus, …

Scavenger Removal Of Bird Carcasses At Simulated Wind Turbines: Does Carcass Type Matter?, Travis L. Devault, Thomas W. Seamans, Kimberly E. Linnell, Dale W. Sparks, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Wind energy development can negatively impact bird populations due to bird–turbine collisions. To accurately estimate bird mortality at wind farms, the number of dead birds found under turbines is commonly corrected for carcass removal by scavengers, which is quantified by measuring persistence of experimental carcasses through time. These studies often use domestic birds as surrogates because carcasses of wild birds (e.g., raptors) are difficult to obtain. We assessed scavenger removal of carcasses from five bird species at simulated turbines to determine whether domestic surrogates are scavenged at a different rate than raptors, species of interest for wind turbine mortality. The …

Testing Of An Oral Dosing Technique For Double-Crested Cormorants, Phalacocorax Auritus, Laughing Gulls, Leucophaeus Atricilla, Homing Pigeons, Columba Livia, And Western Sandpipers, Calidris Mauri, With Artificially Weather Mc252 Oil, Karen M. Dean, Dave Cacela, Michael W. Carney, F. L. Cunningham, C. Ellis, A. R. Gerson, C. G. Guglielmo, Katie C. Hanson-Dorr, K. E. Harr, Katherine A. Healy, Katherine E. Horak, John P. Isanhart, L. V. Kennedy, J. E. Link, I. Lipton, Andrew K. Mcfadden, J. K. Moye, C. R. Perez, C. A. Pritsos, Karen L. Pritsos, Thivanka Muthumalage, S. A. Shriner, S. J. Bursian

United States Department of Agriculture Wildlife Services: Staff Publications

Scoping studies were designed to determine if double-crested cormorants (Phalacocorax auritus), laughing gulls (Leucophaues atricilla), homing pigeons (Columba livia) and western sandpipers (Calidris mauri) that were gavaged with a mixture of artificially weathered MC252 oil and food for either a single day or 4–5 consecutive days showed signs of oil toxicity. Where volume allowed, samples were collected for hematology, plasma protein electrophoresis, clinical chemistry and electrolytes, oxidative stress and organ weigh changes. Double-crested cormorants, laughing gulls and western sandpipers all excreted oil within 30 min of dose, while pigeons regurgitated within less …

Panmixia And Limited Interspecific Introgression In Coyotes (Canis Latrans) From West Virginia And Virginia, Usa, Justin H. Bohling, Lauren L. Mastro, Jennifer R. Adams, Eric M. Gese, Sheldon F. Owen, Lisette P. Waits

United States Department of Agriculture Wildlife Services: Staff Publications

The expansion of coyotes (Canis latrans) into the eastern United States has had major consequences for ecological communities and wildlife managers. Despite this, there has been little investigation of the genetics of coyotes across much of this region, especially outside of the northeast. Understanding patterns of genetic structure and interspecific introgression would provide insights into the colonization history of the species, its response to the modern environment, and interactions with other canids. We examined the genetic characteristics of 121 coyotes from the mid-Atlantic states of West Virginia and Virginia by genotyping 17 polymorphic nuclear DNA microsatellite loci. These …

Frightening Devices, Michael L. Avery, Scott J. Werner

United States Department of Agriculture Wildlife Services: Staff Publications

By their nature, avian frightening devices are intended to provide temporary (days, weeks) relief from a specific depredation or conflict situation. Ideally, the method applied will produce an immediate fright response, causing depredating birds to leave and to stay away as long as the method is in place. Longer-term (months, years) resource protection would involve methods such as crop varietal improvement, blackbird population management, or habitat manipulation. Frightening devices primarily affect the avian auditory and visual senses. With few exceptions (e.g., avian distress or alarm calls), frightening devices are not species-specific.Very few frightening devices have been subjected to adequate scientific …

Low Viral Doses Are Sufficient To Infect Cottontail Rabbits With Avian Influenza A Virus, J. Jeffrey Root, Susan A. Shriner, Jeremy W. Ellis, Kaci K. Vandalen, Heather J. Sullivan

United States Department of Agriculture Wildlife Services: Staff Publications

Influenza A viruses (IAVs) have been reported in wild lagomorphs in environments where they share resources with waterfowl. Recent studies have conclusively shown that a North American lagomorph, cottontail rabbits (Sylvilagus sp.), become infected following exposure to IAVs and can shed significant quantities of virus. However, the minimum infectious dose and the efficiency of various routes of infection have not been evaluated. Thirty-six cottontail rabbits were used in a dose response study assessing both the oral and nasal routes of infection. The nasal route of infection proved to be the most efficient, as all cottontail rabbits shed viral RNA …

Demographic And Temporal Variations In Immunity And Condition Of Polar Bears (Ursus Maritimus) From The Southern Beaufort Sea, Lorin A. Neuman-Lee, Patricia A. Terletzky, Todd C. Atwood, Eric M. Gese, Geoffrey D. Smith, Sydney Greenfield, John Pettit, Susannah S. French

United States Department of Agriculture Wildlife Services: Staff Publications

Assessing the health and condition of animals in their natural environment can be problematic. Many physiological metrics, including immunity, are highly influenced by specific context and recent events to which researchers may be unaware. Thus, using a multifaceted physiological approach and a context-specific analysis encompassing multiple time scales can be highly informative. Ecoimmunological tools in particular can provide important indications to the health of animals in the wild.We collected blood and hair samples from free-ranging polar bears (Ursus maritimus) in the southern Beaufort Sea and examined the influence of sex, age, and reproductive status on metrics of immunity, …

The Roles Of Habitat And Intraguild Predation By Coyotes On The Spatial Dynamics Of Kit Foxes, Robert C. C. Lonsinger, Eric M. Gese, Larissa L. Bailey, Lisette P. Waits

United States Department of Agriculture Wildlife Services: Staff Publications

Intraguild predation (IGP) by a dominant predator can drive the spatial dynamics of a subordinate predator and may explain space-use patterns that deviate from theoretical predictions that species will use areas that maximize the availability of limited resources (resource availability hypothesis). Intraguild predation may suppress the distribution and abundance of mesopredators, but spatial resource partitioning may facilitate coexistence, with the subordinate carnivore utilizing suboptimal habitats. In arid systems, free-standing water was historically scarce, limiting the distribution of larger-bodied predators and offering large areas of refugia for smaller, arid-adapted species, such as the kit fox (Vulpes macrotis). In these …

The Future Of Blackbird Management Research, Page E. Klug

United States Department of Agriculture Wildlife Services: Staff Publications

Human society values birds for their intrinsic and aesthetic value as well as the ecosystem services they provide as pollinators, consumers of pests, and distributors of nutrients and seeds (Wenny et al. 2011). At the same time, conflict between birds and humans is an age-old phenomenon that has persisted as society has transformed and the scale of agriculture has expanded (Conover 2002). Managing conflict between birds and agriculture is challenging for many reasons. Foremost, the need to consider both human welfare and conservation of protected bird species is paramount, with nonlethal management methods preferred to lethal measures from societal, economical, …

Coyote (Canis Latrans) Diet In An Urban Environment: Variation Relative To Pet Conflicts, Housing Density, And Season, S. A. Poessel, E. C. Mock, S. W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

Coyotes (Canis latrans Say, 1823) are highly successful in urbanized environments, but as they populate cities, conflict can occur and often manifests in the form of incidents with pets. To better understand whether coyotes view pets as prey or, alternatively, as competitors or a threat, we conducted a diet analysis of coyotes in the Denver metropolitan area (DMA) by analyzing scats. We also examined differences in diet between high- and low-density housing and among seasons. We found only small percentages of trash and domestic pets in the coyote diet. The presence of pets in the diet did not coincide …

Human Development And Climate Affect Hibernation In A Large Carnivore With Implications For Human–Carnivore Conflicts, Heather E. Johnson, David L. Lewis, Tana L. Verzuh, Cody F. Wallace, Rebecca M. Much, Lyle K. Willmarth, Stewart W. Breck

United States Department of Agriculture Wildlife Services: Staff Publications

1. Expanding human development and climate change are dramatically altering habitat conditions for wildlife. While the initial response of wildlife to changing environmental conditions is typically a shift in behavior, little is known about the effects of these stressors on hibernation behavior, an important life-history trait that can subsequently affect animal physiology, demography, interspecific interactions and human-wildlife interactions. Given future trajectories of land use and climate change, it is important that wildlife professionals understand how animals that hibernate are adapting to altered landscape conditions so that management activities can be appropriately tailored.

2. We investigated the influence of human development …

Large Carnivore Science: Non-Experimental Studies Are Useful, But Experiments Are Better, Benjamin L. Allen, Lee R. Allen, Henrik Andrén, Guy Ballard, Luigi Boitani, Richard M. Engeman, Peter J. S. Fleming, Adam T. Ford, Peter M. Haswell, Rafal Kowalczyk, John D. C. Linnell, L. David Mech, Daniel M. Parker

United States Department of Agriculture Wildlife Services: Staff Publications

1. Response to Bruskotter and colleagues

We recently described the following six interrelated issues that justify questioning some of the discourse about the reliability of the literature on the ecological roles of large carnivores (Allen et al., in press):

1. The overall paucity of available data,

2. The reliability of carnivore population sampling techniques,

3. The general disregard for alternative hypotheses to top-down forcing,

4. The lack of applied science studies,

5. The frequent use of logical fallacies,

6. The generalisation of results from relatively pristine systems to those substantially altered by humans.

We thank Bruskotter et al. (2017) for …

Adaptation Of The Aesop’S Fable Paradigm For Use With Raccoons (Procyon Lotor): Considerations For Future Application In Non‑Avian And Non‑Primate Species, Lauren Stanton, Emily Davis, Shylo Johnson, Amy T. Gilbert, Sarah Benson-Amram

United States Department of Agriculture Wildlife Services: Staff Publications

To gain a better understanding of the evolution of animal cognition, it is necessary to test and compare the cognitive abilities of a broad array of taxa. Meaningful interspecies comparisons are best achieved by employing universal paradigms that standardize testing among species. Many cognitive paradigms, however, have been tested in only a few taxa, mostly birds and primates. One such example, known as the Aesop’s Fable paradigm, is designed to assess causal understanding in animals using water displacement. To evaluate the universal effectiveness of the Aesop’s Fable paradigm, we applied this paradigm to a previously untested taxon, the raccoon ( …

Coyote Removal: Can The Short-Term Application Of A Controversial Management Tool Improve Female Greater Sage-Grouse Survival Or Nest Success?, Elizabeth K. Orning, Julie K. Young

United States Department of Agriculture Wildlife Services: Staff Publications

Greater sage-grouse Centrocercus urophasianus have declined across western North America, intensifying the need for ecological research that enhances management and conservation goals. Predator–prey interactions can have widespread ecological effects but there is a paucity of information about predator effects on sage-grouse ecology. During a two-year study from 2011–2012, we modified the existing framework designed for predator management to test the effects of coyote Canis latrans removal on female sage-grouse survival and nest success in the Bighorn Basin of Wyoming, USA, where coyotes were found to be the dominant predator. We used VHF radio-telemetry to monitor female survival and locate nests …

Attractants For Wild Pigs: Current Use, Availability, Needs, And Future Potential, Michael J. Lavelle, Nathan P. Snow, Justin W. Fischer, Joe M. Halseth, Eric H. Vannatta, Kurt C. Vercauteren

United States Department of Agriculture Wildlife Services: Staff Publications

Populations of wild pigs (Sus scrofa) are burgeoning around the globe, and they are currently the target of extensive research and management efforts.Wild pigs are a highly successful invasive species that cause extensive damage to agriculture and native plant and animal communities. Lethal control is the most common management strategy used to reduce wild pig populations and their damage, and many lethal strategies rely on luring wild pigs to a specific location. Most commonly, a food commodity such as corn or maize (Zea mays) is used for attracting wild pigs to a site, but baiting with …

Potential Effects Of Incorporating Fertility Control Into Typical Culling Regimes In Wild Pig Populations, Kim M. Pepin, Amy J. Davis, Fred L. Cunningham, Kurt C. Vercauteren, Douglas C. Eckery

United States Department of Agriculture Wildlife Services: Staff Publications

Effective management of widespread invasive species such as wild pigs (Sus scrofa) is limited by resources available to devote to the effort. Better insight of the effectiveness of different management strategies on population dynamics is important for guiding decisions of resource allocation over space and time. Using a dynamic population model, we quantified effects of culling intensities and time between culling events on population dynamics of wild pigs in the USA using empirical culling patterns and data-based demographic parameters. In simulated populations closed to immigration, substantial population declines (50±100%) occurred within 4 years when 20±60% of the population …

Effects Of Scale Of Movement, Detection Probability, And True Population Density On Common Methods Of Estimating Population Density, David A. Keiter, Amy J. Davis, Olin E. Rhodes Jr., Fred L. Cunningham, John C. Kilgo, Kim M. Pepin, James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

Knowledge of population density is necessary for effective management and conservation of wildlife, yet rarely are estimators compared in their robustness to effects of ecological and observational processes, which can greatly influence accuracy and precision of density estimates. In this study, we simulate biological and observational processes using empirical data to assess effects of animal scale of movement, true population density, and probability of detection on common density estimators. We also apply common data collection and analytical techniques in the field and evaluate their ability to estimate density of a globally widespread species. We find that animal scale of movement …

Restricted Cross-Scale Habitat Selection By American Beavers, Robert A. Francis, Jimmy D. Taylor, Eric Dibble, Bronson Strickland, Vanessa M. Petro, Christine Easterwood, Guiming Wang

United States Department of Agriculture Wildlife Services: Staff Publications

Animal habitat selection, among other ecological phenomena, is spatially scale dependent. Habitat selection by American beavers Castor canadensis (hereafter, beaver) has been studied at singular spatial scales, but to date no research addresses multi-scale selection. Our objectives were to determine if beaver habitat selection was specialized to semiaquatic habitats and if variables explaining habitat selection are consistent between landscape and fine spatial scales. We built maximum entropy (MaxEnt) models to relate landscape-scale presence-only data to landscape variables, and used generalized linearmixed models to evaluate fine spatial scale habitat selection using global positioning system (GPS) relocation data. Explanatory variables between the …

Effective Dose And Persistence Of Rhodamine-B In Wild Pig Vibrissae, Sarah C. Webster, Fred L. Cunningham, John C. Kilgo, Mark A. Vukovich, Olin E. Rhodes Jr., James C. Beasley

United States Department of Agriculture Wildlife Services: Staff Publications

As a result of substantial ecological and economic damage attributed to wild pigs (Sus scrofa), there is international interest in using pharmaceutical baits to control populations. To assess the efficacy and specificity of baiting programs, chemical biomarkers can be used to evaluate uptake of pharmaceutical baits. Rhodamine B (RB) is known to be an effective biomarker in wild pigs. However, significant data gaps exist regarding the minimum effective dosage and persistence of RB in wild pigs. We used a controlled doubleblind study experiment conducted in spring of 2014 on the Savannah River Site, Aiken, South Carolina, USA, wherein …

The Persistence Of Multiple Strains Of Avian Influenza In Live Bird Markets, Amy Pinsent, Kim M. Pepin, Huachen Zhu, Yi Guan, Michael T. White, Steven Riley

United States Department of Agriculture Wildlife Services: Staff Publications

Multiple subtypes of avian influenza (AI) and novel reassortants are frequently isolated from live bird markets (LBMs). However, our understanding of the drivers of persistence of multiple AI subtypes is limited. We propose a stochastic model of AI transmission within an LBM that incorporates market size, turnover rate and the balance of direct versus environmental transmissibility. We investigate the relationship between these factors and the critical community size (CCS) for the persistence of single and multiple AI strains within an LBM. We fit different models of seeding from farms to two-strain surveillance data collected from Shantou, China. For a single …

Serologic Evidence Of Various Arboviruses Detected In White Tailed Deer (Odocoileus Virginianus) In The United States, Kerri Pedersen, Eryu Wang, Scott C. Weaver, Paul C. Wolf, Adam R. Randall, Kyle R. Van Why, Amelia P.A. Travassos Da Rosa, Thomas Gidlewski

United States Department of Agriculture Wildlife Services: Staff Publications

White-tailed deer (Odocoileus virginianus) are an abundant mammal with a wide geographic distribution in the United States, which make them good sentinels for monitoring arboviral activity across the country. Exposure to various arboviruses has been detected in white-tailed deer, typically in conjunction with another diagnostic finding. To better assess the exposure of white-tailed deer to seven arboviruses, wetested 1,508 sera collected from 2010 to 2016 for antibodies to eastern equine encephalitis (2.5%), Powassan (4.2%), St. Louis encephalitis, (3.7%), West Nile (6.0%), Maguari (19.4%), La Crosse (30.3%), and bluetongue (7.8%) viruses. At least one arbovirus was detected in 51.3%, …