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Development Of Novel Analytical Methods For The Investigation Of Mammalian Tissues And Cells, Grace A. Binder

USF Tampa Graduate Theses and Dissertations

This dissertation endeavors to advance the frontiers of analytical chemistry by addressing critical challenges across three distinct chapters. In the first chapter, the focus is on the evaluation of digestion methods for the analysis of trace metals in mammalian tissues and the Standard Reference Material NIST 1577c. The primary goal is to optimize methodologies, laying the groundwork for reliable trace metal analysis and furthering our understanding of their roles in biological systems.

Transitioning into the realm of immunomodulation, the second chapter elucidates immune response, disease and treatment through the optimization and discovery of STING modulators, utilizing the luciferase assay for …

A Republic Of Pawprints: The Rise And Reign Of The Alaskan Sled Dog, 1870-1970, Christopher David Adkins

USF Tampa Graduate Theses and Dissertations

This dissertation charts the career of the Alaskan sled dog: how it was used as an engine of development, how it became a symbol for early American Alaska, and how it fell victim to obsolescence as newer and faster means of conveyance became available. The figure of the sled dog in the Arctic is complex. Because it was closer to the wolf in phenotype both it, and Alaska, were buffeted by the same White settler expectations and Darwinian chauvinism which afflicted its Indigenous peoples. This would intensify during the baptisms by fire of the gold rushes. Dog abuse – overwork, …

Time-Scale Of Groundwater Recharge In Coastal Plain Soils, Qing Du

USF Tampa Graduate Theses and Dissertations

Understanding and modeling the timing and magnitude of groundwater recharge fromrainfall infiltration through vadose zone percolation is important for many reasons but especially because the flux is being acted on by root zone evapotranspiration (ET) and very little of rainfall infiltration ever becomes water table recharge. This study elaborates on the considerable time of the wetting front arrival and ultimate bulk recharge of rainfall infiltration in shallow water table fine-sandy soils typical of coastal plain environments such as Florida. Calibrated Hydrus-1D modeling of Florida (Myakka) soil was evaluated at varying depths of water table and hydraulic conductivities to bracket the …

Context-Aware Affective Behavior Modeling And Analytics, Md Taufeeq Uddin

USF Tampa Graduate Theses and Dissertations

Affective computing (AC) is a sub-domain of AI that has the potential to assist people by assessing mental states and making appropriate recommendations to patients, loved ones, caregivers, and domain experts. Humans usually produce an enormous amount of data (such as face videos) every day. One of the major challenges for affective computer vision is to efficiently deal with high volumes of data to facilitate automated model development. To cope with this challenge, we developed computer vision algorithms that measure the expressivity of the human face from video data. More precisely, the developed algorithms can map complex affect information from …

Seminoles, Soldiers, And Settlers: Identity And Power On The Florida Frontier, Jean Louise Lammie

USF Tampa Graduate Theses and Dissertations

This project uses an historical archaeological approach to analyze the processes of cultural negotiation between disparate social groups living in a remote borderland in the early to mid-nineteenth centuries. Florida, first colonized by the Spanish in the 1500s was passed to the United States in 1821 as part of the young nation’s nascent Manifest Destiny. The Seminoles experienced multiple colonial conflicts, dealt with new diseases, and selected aspects of European culture to incorporate into their daily practices of lifeways.

Archaeological studies of culture change tend to focus on the product of colonial interactions rather than the processes of change that …

Quandle Rings, Idempotents And Cocycle Invariants Of Knots, Dipali Swain

USF Tampa Graduate Theses and Dissertations

Quandles are sets with self-distributive binary operations that axiomatize the three Reidemeister movesin classical knot theory. In an attempt to bring ring theoretic techniques to the study of quandles, a theory of quandle rings analogous to the classical theory of group rings where several interconnections between quandles and their associated quandle rings have been explored. Functoriality of the construction implies that morphisms of quandle rings give a natural enhancement of the well-known quandle coloring and quandle 2 cocycle invariant of knots and links.

The dissertation is structured into two main parts. In the first part, we delve into quandle rings …

Narrative Language Assessment And Intervention: Applications With Children With And Without Disabilities, Norah M. Almubark

USF Tampa Graduate Theses and Dissertations

Narrative is the art of conveying a sequence of causally and temporally linked events through monologic telling. Various functions can be served through narratives including sharing experiences, expressing ideas, providing entertainment, and imparting cultural or social values. Narrative language represents an authentic type of language that is useful for assessment and intervention because it can be analyzed according to content and form. The ability to use complex narrative language and produce quality narratives is associated with long-term academic and social benefits (Uccelli et al., 2019; Suggate et al, 2018). Given this crucial link, narrative language is suitable for child language …

Local Ecological Knowledge, Education, And Bilingualism: Ethnography Of A Stem Program For Elementary Students In Southwestern Florida, Barbara D. Herrera

USF Tampa Graduate Theses and Dissertations

In response to the limited inclusion of cultural relevant pedagogy in Science, Technology, Engineering, and Mathematics (STEM) centered educational programs, this study illustrates dynamics that encourage inclusivity and provide a space for minority students to develop self-efficacy while also exposing them to formal modes of STEM knowledge. Elementary level English/Spanish speaking children were taught local ecological knowledge (LEK) during a STEM summer program in a city in Southwestern Florida. The use of participant observation, group interviews with the students, and interviews with the educators allowed for an in-depth analysis of the program and the established goals. This study provides insight …

Beach Processes Controlling Erosional Hotspots And Their Influences On Beach Nourishment Performances: A Case Study Along West-Central Florida Barrier Island Beaches, Sophia Gutierrez

USF Tampa Graduate Theses and Dissertations

This study examines the long-term performance of repeated beach nourishment over an approximately 35-year period at two barrier islands, Sand Key and Treasure Island along the west-central Florida coast, USA. Five cycles of beach nourishment on Sand Key and six on Treasure Island were conducted during the study period. Three erosional hotspots were identified along a combined 14-km stretch of the nourished beach. The processes that cause the erosional hotspot were examined through numerical modeling of nearshore wave and current fields.

The most recent three nourishments since 2006 were monitored systematically by this study. Beach profiles were surveyed bimonthly to …

Comparative Analysis Of Time Series Models On U.S. Stock And Exchange Rates: Bayesian Estimation Of Time Series Error Term Model Versus Machine Learning Approaches, Young Keun Yang

USF Tampa Graduate Theses and Dissertations

This study presents a comparative analysis of contemporary applications of time series models, focusing on the Bayesian approach. In contrast to many nonparametric studies, the Bayesian approach circumvents the common issue of bandwidth selection by offering systematic estimation and avoiding ad hoc methods. Specifically, we delve into the Bayesian approach for estimating the autocovariance function of a time series model’s error term. Traditional time series models often make the unrealistic assumption of a constant error term. Furthermore, models such as autoregressive conditional heteroskedasticity (ARCH) and general autoregressive conditional heteroskedasticity (GARCH) address the limitation of constant variance by assuming an autoregressive …

"A Historic Place Of Peace And Reflection": A Critical Analysis Of Digital Methods In The Recovery Of Forgotten Black Cemeteries, Sofia M. Almeida

USF Tampa Graduate Theses and Dissertations

This thesis is a critical analysis of digital methods employed as part of a growing movement to identify, record, preserve, and research historic Black cemeteries. Through the joint partnership of the Black Cemetery Network (BCN) and the Institute for Digital Exploration (IDEx), a digital public mortuary archaeology approach was applied to digitally preserve Mount Carmel Cemetery in Pasco County, Florida. The digitization project resulted in the production of digital images, 3D models, and updated site maps of the cemetery and the memorials within. In all, ten gravestones in various conditions were identified, digitized, and turned into 3D models. The remains …

Rate Of Latine Caregiver Involvement In The Well-Being Promotion Program Using Culturally And Linguistically Responsive Communication Practices, Nicole G. Pacateque Rodriguez

USF Tampa Graduate Theses and Dissertations

Latine and Hispanic youth tend to have higher rates of mental health disturbances in school compared to non-Latine youth, yet they are less likely to receive support (Alegría et al., 2008). Additionally, Latine families are more likely to terminate treatment for youth early due to negative stigma, lack of culturally appropriate mental health care, racial and ethnic discrimination, and linguistic and cultural barriers involving care (Kapke & Gerdes, 2016). Best practices in school-based mental health services often involve including caregivers in care (Hoover et al., 2019) as it can benefit outcomes of treatment for the child (Richards et al., 2007). …

Advancing Text Summarization And Classification: Deep Insights From Transformer-Based Statistical Learning, Kun Bu

USF Tampa Graduate Theses and Dissertations

Artificial Intelligence (AI) is a part of human's daily life nowadays. Machine Learning (ML) as one aspect from AI has been rapidly developing during the past two decades, especially from the statistical learning approaches, which emphasized the use of probability and statistics to model data, such as Support Vector Machines (SVMs) for classification and regression tasks to the ensemble learning techniques, such as Random Forest, Gradient Boosting Machine (GBM), and stacking. Ensemble learning has evolved into a pivotal concept in contemporary machine learning, empowering practitioners to amalgamate multiple models to enhance generalization, accuracy, and robustness. As the field of machine …

Iron Isotope Transformations In Saanich Inlet, Claire Onak

USF Tampa Graduate Theses and Dissertations

Iron (Fe) is required for many biogeochemical processes, with Fe bioavailability and chemistry being controlled by redox reactions that transform Fe between oxidation states and dissolved and particulate phases. In oxic seawater, Fe is present in the Fe(III) oxidation state, mainly as Fe oxyhydroxides. Under anoxic marine conditions, such as margin sediments under oxygen minimum zones or restricted basins with anoxic bottom water, Fe(III) is reduced to Fe(II), which is highly soluble and can be present at high concentrations ([Fe]). Fe isotope ratios (δ56Fe relative to IRMM-14) can be used to characterize Fe redox transformations, constrain Fe sources of Fe …

The Thermodynamics And Structure Of Tumor Suppressor P53 And Helical Mutants Binding To Inhibitors Mdm2 And Mdmx At The Primary And Secondary Sites, Pirada Serena Higbee

USF Tampa Graduate Theses and Dissertations

p53 is an intrinsically disordered protein that is a tumor suppressor. The transactivation domain is split into two parts (TAD1 and TAD2). These TADs bind to inhibitors MDM2, and a homolog, MDMX, but TAD1 binds with greater affinity. MDM2 is an E3 ubiquitin ligase that leads to the degradation of p53. MDMX is not an E3 ubiquitin ligase, but it does prevent p53 from binding to DNA, thus preventing its tumor suppressor role.

Previous research showed that increasing p53 helicity by mutating prolines to alanines increased p53's helicity but not the length of the helix. The increase in helicity increased …

Effectiveness Of Contact-Based Strategies On Improving Mental Illness Stigma And Help-Seeking Behaviors Within Families, B. Michelle Wilks

USF Tampa Graduate Theses and Dissertations

Adolescence is a developmental period that often involves extreme physical, psychological, and emotional changes and sometimes the experience of significant personal and social challenges. In addition to these challenges, youth are trying to find their place in the world and developing their identities, which depend heavily on their relationships with others. Adolescents who struggle with their mental health and also fail to develop meaningful connections with others, tend to have poor self-esteem, self-isolate, are ostracized by others, and have a poor social self-concept, all of which are associated with poor mental health and increased risk for suicidal ideation. The impact …

Individual Behavioral Modeling Across Games Of Strategy, Logan Fields

USF Tampa Graduate Theses and Dissertations

An individual’s actions in a particular environment and with specified resources can reveal their decision-making tendencies and patterns, and by analyzing the variations in cognitive traits among individuals, it may be possible to identify trends that can foretell their future behaviors. This can be a powerful tool in various fields including cognitive modeling, player analytics, computer security, and threat detection. Collectible card games are a fruitful test space for studying cognitive differences in decision-making, as they can have clearly defined and replicable environments and large player bases. As such, in this work, I explore the potential of using two virtual …

Sociocultural Factors, Definitions, And Experiences Of Intimate Partner Violence Among Latina And Hispanic Women, Vanessa Centelles

USF Tampa Graduate Theses and Dissertations

Intimate partner violence (IPV) is a pressing public health concern in the United States (US), particularly for women. Some studies have shown that Hispanic and Latina women are at an elevated risk of experiencing IPV due to a notable passiveness of violence among traditional Hispanic and Latina women. Thus, it is crucial for research to examine the effects of sociocultural variables—such as cultural orientation, gender norm beliefs, and ethnic identity—on IPV within this demographic, particularly for women in the US. Furthermore, while the experiences of IPV victimization among Hispanic and Latina women have begun to be recognized in the broader …

It's Real, It Just Hasn't Happened Yet: A Three-Article Dissertation On Emerging Technologies In Higher Education, Jeanette Abrahamsen

USF Tampa Graduate Theses and Dissertations

The purpose of this three-article dissertation is to better understand the role emerging technologies are playing in learning. This dissertation explores tools on a spectrum of reality from fully immersive virtual reality (VR), semi-immersive VR, non-immersive VR, augmented reality (AR), mixed reality (MR), and extended reality (XR). Three studies focus on emerging technologies from the student, educator, researcher perspectives to develop a holistic picture of emerging technologies in higher education. I explore factors that impact new technology adoption, which includes advantages and disadvantages to deploying different digital tools. This dissertation begins with a systematic literature review of 20 research studies …

Beliefs Of Male Elementary School Special Education And General Education Teachers Regarding Full Inclusion For Students With Autism Spectrum Disorder In Saudi Arabia, Sultan Alanazi

USF Tampa Graduate Theses and Dissertations

AbstractThe current educational settings for students with Autism Spectrum Disorders (ASD) in Saudi Arabia consist of special institutions for students with intellectual disabilities and special classrooms within public schools. The Ministry of Education in Saudi Arabia is interested in full inclusion for students with ASD because of the social, psychological, and educational benefits it can provide them. One crucial factor for achieving full inclusion is considering elementary teachers’ perceptions and beliefs toward full inclusion, as they are one of the primary stakeholders in this venture. Therefore, elementary general education and special education teachers’ beliefs regarding full inclusion for students with …

Thermal Ecology And Swimming Performance Of Native Tadpoles Dryophytes Femoralis In Central Florida, Jessalyn Aretz

USF Tampa Graduate Theses and Dissertations

Urbanization and climate warming have contributed to global amphibian declines in recent decades. Amphibians are particularly vulnerable to climate and urban-induced warming because their physiological processes are dependent on temperature across all life stages, but few studies have been done on tadpole responses to warming in comparison to adult responses. The study objective was to determine how the thermal ecology and swimming performance of a native Florida tadpole varied with rearing temperature and urban level, and whether these traits are plastic or adaptive. We collected eggs from wild populations of pine woods treefrog (Dryophytes femoralis) tadpoles at an urban and …

The Integration Of Assistive Technology By Female In-Service Teachers Of Students With Learning Disabilities In Saudi Arabia: A Qualitative Interview Study, Badriah Alotaiby

USF Tampa Graduate Theses and Dissertations

The aim of this study is to explore the teachers’ perceptions of integrating assistivetechnology into the curriculum for students with learning disabilities in Saudi Arabia. It is known that teaching students with learning disabilities had been one of the significant difficulties in the past and nowadays. Therefore, integrating assistive technology into the educational process would make it easier for the teachers of students with learning disabilities to teach these students through the use of integrating assistive technology. The use of such assistive technologies will be beneficial for learning disabilities students as well. The main question guiding this research is: What …

Advancing Depth-Storage-Discharge Modeling In Regional Hydrology, Fahad Alshehri

USF Tampa Graduate Theses and Dissertations

This dissertation presents the development of an innovative approach to populating rating characteristics and supporting hydrologic modeling, designed to simplify complex real-world hydrological systems and accurately estimate their responses to rainfall, runoff, baseflow and evaporation stresses. The core of this research addresses the challenges inherent in characterizing hydrography elements in hydrologic modeling, particularly in regions lacking comprehensive stream reach survey data, flow and stage. This issue is pronounced in areas with extensive wetland hydrography, where traditional modeling requires intensive manual calibration, and course rating data that are often unavailable. To overcome these challenges, this study introduces a novel procedure that …

An Ecofeminist Ontological Turn: Preparing The Field For A New Ecofeminist Project, M. Laurel-Leigh Meierdiercks

USF Tampa Graduate Theses and Dissertations

Ecofeminists have predominantly refuted oppressive conceptual frameworks by rejecting dualistic logic and value hierarchies, despite the foundational view of ontology that underlies our logic and value systems. I argue that ecofeminists must engage in the creative process of articulating an ecofeminist ontology. I demonstrate that the omission of ontological discourse in ecofeminist scholarships is primarily due to the time period during which ecofeminism gained peak traction and their concerns with essentialism. I also show that feminists experienced an ontological turn in the 2000s that produced a new model for ontological theories that would be beneficial for ecofeminists to embrace. In …

Making The Invisible Visible: (Re)Envisioning The Black Body In Contemporary Adaptations Of Nineteenth-Century Fiction, Urshela Wiggins Mckinney

USF Tampa Graduate Theses and Dissertations

A trend in neo-Victorian adaptations -- both novels and films-- that reimage, and at times reinterpret, canonical Victorian texts is the inclusion of nonwhite, mainly Black, perspectives, which has gained considerable traction in recent years. A vital aspect of this trend is the purposeful attempt to re-establish iconic Victorian characters through Black characterizations. In doing so, filmmakers and authors are reinvigorating familiar texts to provide an inclusionary space for the Black experience previously ignored in the original texts. These adaptations, which revisit and often reinterpret Victorian fiction, have undergone notable transformations by incorporating Black characters to fill voids in traditional …

Band Members’ Attitudes Toward The Queer Community And Perception Of Band As A Safe Space, Brian J. Panetta

USF Tampa Graduate Theses and Dissertations

Using Bronfenbrenner’s ecological framework of human development and Yuval-Davis’ analytical framework of belonging as a lens to explain how the band environment might cultivate positive attitudes toward the queer community, this study investigated band members’ attitudes toward the queer community and their perception of the band as a safe space. Utilizing adapted inventories with established reliability and validity, the following research questions were addressed: 1) What are queer and non-queer band members’ attitudes toward the queer community? 2) What are queer and non-queer band members’ attitudes toward the band as a safe space? 3) What differences exist between the independent …

Stereotyped Non-Stereotypicality: Exploring Positive Effects Of Non-Stereotypical Gender Portrayals On Consumer Perceptions, Veronika Ponomarenko

USF Tampa Graduate Theses and Dissertations

This research attempts to identify contexts where non-stereotypical gender portrayals may have positive effects on consumer perceptions. It tests the perceived self-expressiveness of non-stereotypical gender portrayals as the mediating mechanism behind these effects as well as other alternative mechanisms in a comprehensive experiment. The analysis results show that an interpretation of gender through the theoretical lens of extravagance rather than self-expressiveness seems to be more appropriate. This study also introduces an overarching theoretical framework of metacognitive experiences that may explain the processes driving the effects of non-stereotypical gender portrayals on consumer perceptions. Finally, this research explores different types of gender …

Hidden Monstrosities: The Transformation Of Medieval Characters And Conventions In Shakespeare's Romances, Lynette Kristine Kuliyeva

USF Tampa Graduate Theses and Dissertations

When Shakespeare’s First Folio was published in 1623, it was entitled Mr. William Shakespeare’s Comedies, Histories, and Tragedies, the title designating the three genres under which his plays would be categorized for the next 250 years. Later, Irish critic Edward Dowden took it upon himself to restructure the Shakespearean canon by adding plays that were not previously published in the First Folio, reclassifying the genres of several of the plays, and establishing a new genre to accompany the previous three: romance. Within this fourth generic category of romance, Dowden situated four of the Shakespearean plays: Pericles, Prince of Tyre; Cymbeline; …

Spatial Patterns Of Building Damages And Associated Socio-Economic Factors By Hurricane Ian, Md Zakaria Salim

USF Tampa Graduate Theses and Dissertations

Literature shows that communities with different socio-economic conditions suffer from different levels of damage in disasters. In addition to the physical intensity of hazards, such disparities are also related to varying abilities to prepare for and respond to natural hazards. The study analyzes the spatial patterns of building damage in Hurricane Ian in 2022 and investigates the socio-economic disparities related to building damage. Specifically, this study employs NASA’s Damage Proxy Map (DPM2) to analyze Ian's spatial patterns of building damage. Then, it uses statistical analysis to assess the relationship between building damage and various physical and socio-economic variables at building …

Introductory Geology Laboratory Students' Learnings From And Perceptions Of Physical And Virtual Polarizing Light Microscopes – An Exploratory Mixed Methods Study, Christy M. Bebeau

USF Tampa Graduate Theses and Dissertations

Undergraduate geoscience laboratory courses provide hands-on learning about earth materials. However thin section analysis is not typically included in introductory geology laboratories, likely due to the polarizing light microscope (PLM) costs. Therefore, there is a lack of research about the educational outcomes of novices learning these skills.

In this inquiry, I conducted mixed methods research about novice students' learning of thin section analysis in an introductory geology laboratory and evaluated learning and perception differences between students who used a virtual PLM and those who used a physical PLM. I collected data from Creative Exercise (CE) assessments in student midterm and …