Digital Commons Network^™

Transparent Planar Micro-Electrode Array For In-Vitro Electric Field Mediated Gene Delivery, Raj Himatlal Shah

USF Tampa Graduate Theses and Dissertations

Gene delivery is the process of delivering the modified gene into the human cell. There are two types of gene delivery depending on where the target cells are. The in-vivo gene delivery deals with delivering the altered genes into the cells while still in the host body. While another approach is to extract the cells from the host body, transfer the modified gene into cells and then put these cells back into the host body, this approach is called the ex-vivo method.

A vector is a carrier that carries the modified gene to the host cell or body. Hence, depending …

Recognizing Patterns From Vital Signs Using Spectrograms, Sidharth Srivatsav Sribhashyam

USF Tampa Graduate Theses and Dissertations

Spectrograms extract frequency components from a signal. Spectrograms have beenin use for a long time mainly to analyze frequency components in audio signals. Typically, these audio signals have a very high sampling rate, various frequency components and high frequency variability with time. Vital signs on other hand have very low sampling rate with no frequency variability. This work explores if spectrograms can be used to analyze and recognize patterns from vital signs signals.

As mentioned above, spectrograms deal with frequencies. More the variability of frequency, better the patterns emerge when spectrograms are applied on the signals. As vital signs lack …

Improving Environmental Protection: One Imagined Touch At A Time, Luke Ingalls Liska

USF Tampa Graduate Theses and Dissertations

Can imagined touch of flora and fauna (i.e., the plants and animals of the natural world) make you more willing to support environmental protection efforts? Across seven studies, I demonstrate that by asking consumers to imagine touching fauna, marketers can encourage consumers to become more engaged in environmental protection efforts. This effect occurs because imagined touch generates haptic imagery, which enhances a consumer’s emotional attachment to fauna. I demonstrate that emotional attachment to fauna induced via imagined touch enhances individual’s willingness to share Facebook posts, improves their willingness to support increased fines for environment-related offenses, and increases the number of …

Assessing The Feasibility Of Microbially Managed Biological Filtration In U.S. Drinking Water Systems For Removal Of Contaminants Of Emerging Concern, Andrew J. Black

USF Tampa Graduate Theses and Dissertations

The future of water security in the U.S. is clouded by the progression of anthropogenic climate change and the increasing number of households unable to afford access to safe drinking water, while advances in detection and potential health risks of trace organic contaminants are constantly moving the water treatment finish line. There is a need for sustainable, economically feasible technology to provide the means to meet increasingly stringent water quality regulations while also ensuring current standards are met for all communities. Biologically active filtration, combined with an operational approach designed to optimize contaminant removal by the biofilm, may enable water …

Cetacean Maternal Investment: Importance In Conservation Across Species And Drivers For Interspecific Altruism, Christopher Klein

USF Tampa Graduate Theses and Dissertations

Cetacean maternal investments can be useful for conservation management as well as examining behaviors such as interspecific altruism in the humpback whale, Megaptera novaeangliae. Cetaceans are among the most threatened group of marine mammals. In the second chapter, maternal investments across ~90 species/subspecies of Cetacea were analyzed to aid in the understanding of recovery/replacement for conservation and management. Using Principal Components Analysis (PCA), a relative maternal-investment index for 41 cetacean species was built. The maternal-investment index was a composite of four maternal-investment variables, the duration of gestation, lactation, calving intervals, and the number of reproduction years per female per species. …

Students’ Perceptions Of Factors Contributing To Enrollment In Arabic Courses At A College Level, Qays Q. Majeed

USF Tampa Graduate Theses and Dissertations

The purpose of this evaluation was to investigate the factors that contribute to students’ recruitment and retention in the Arabic program at my college. The two questions guiding this evaluation were: (1) what factors influence students to join the Arabic program at my college and (2) what factors influence students to continue to enroll in subsequent higher-level Arabic courses? To accomplish this evaluation, I conducted interviews with six former students --four women and two men--in the Arabic program. Eight themes emerged following analysis of data. The findings show that students joined the Arabic program for a variety of reasons. Several …

Cytoplasmic Polyadenylation Element Binding Protein 2 Alternative Splicing Regulates Hif1Α During Chronic Hypoxia, Emily M. Mayo

USF Tampa Graduate Theses and Dissertations

Chronic pulmonary hypoxia commonly results in the sustained expression of HIF1 (hypoxia inducible factor 1), a heterodimeric transcription factor, that, if unrestrained, can result in dramatic vasculature remodeling, pulmonary arterial hypertension, and right-sided heart failure. Together, these pulmonary disorders cost approximately $100 billion annually to treat due to the limited therapeutic targets designed to inhibit HIF1 expression. In this study, we introduce a translational regulator of HIF1 expression, known as Cytosolic polyadenylation element binding proteins 2 (CPEB2). Our lab has previously demonstrated in cancer cells that alternatively spliced isoforms of CPEB2 regulate the translation of the HIF1 oxygen-dependent subunit, HIF1α, …

A Constitutive-Based Deep Learning Model For The Identification Of Active Contraction Parameters Of The Left Ventricular Myocardium, Igor Augusto Paschoalotte Nobrega

USF Tampa Graduate Theses and Dissertations

Modern breakthroughs in biomedical engineering, computer science, and data mining have created new opportunities for detecting important mechanical properties of soft tissues that can be employed to identify possible signs of diseases or physiological difficulties. However, the scarcity of different mechanical properties obtained through noninvasive testing emphasizes the importance of incorporating authentic biological data into computer models capable of replicating the behavior of soft tissues.

The field of continuum theory of large deformation hyperactivity permits the formulation of highly descriptive mathematical research and computational models capable of perfectly describing the minute mechanical characteristics of soft materials. By including features about …

Home Range Area, Overlap, Homing Behavior And Movement Patterns In Plestiodon Reynoldisi, Paul Wieczorek Ii

USF Tampa Graduate Theses and Dissertations

Home range and home range overlap information are crucial to create management plans for species of conservation concern. It provides information as to how much land a viable population needs to survive. The South Florida Multi-Species Recovery Plan highlights the need for home range and movement information for Plestiodon reynoldsi (Florida Sand Skink), a threatened lizard species precinctive to Florida Scrub habitat. We investigated home range sizes, their relationship with SVL and mass, home range overlap, homing behavior, and movement patterns of the Florida Sand Skink (FSS) and over a three-year period in the Lake Wales Ridge National Wildlife Refuge. …

Saudi High School Science Teachers' Perceptions Towards The Integration Of Digital Game-Based Learning Into Their Teaching Practice, Jawaher A. Alsultan

USF Tampa Graduate Theses and Dissertations

The switch to distant learning due to the COVID-19 pandemic demanded an immediate adaptation of technology platforms to improve students' online learning. Science teachers were suddenly required to teach their classes virtually with little time and preparation. This breakneck shift to remote learning raised the question of how teachers can provide instruction to their students in the practices of science using virtual technologies. This embedded case study investigates an intervention to support Saudi science teachers facing the unprecedented challenge posed by COVID-19 in teaching the practices of science in the virtual school platform, Madrasati, through the engagement in digital game-based …

Maximum Multiplicative Programming: Theory, Algorithms, And Applications, Payman Ghasemi Saghand

USF Tampa Graduate Theses and Dissertations

This dissertation presents three different contributions to an important class of optimization problems known as Multiplicative Programs (MPs). The first group of contributions contains the development and analysis of several multi-objective optimization-based based algorithms designed to find the optimal solution of Mixed Integer Linear MPs. As for the second group, the application of a special class of MPs in radiotherapy planning is presented. Finally, in the last group, a new technique for conducting the multiplication process in the objectives of MPs is presented. Using this technique, we introduce a family of novel solution methods that are capable of solving both …

Low-Cost Device For Laser Phacoemulsification: A Preliminary Study, Mitchell Harrah

USF Tampa Graduate Theses and Dissertations

The current gold standard of cataract removal surgery is phacoemulsification systems. These are divided based on the type of devices used; both are considered equal but with different delivery modes. These delivery methods are either acoustic or light-based. However, these devices pose two hurdles for impoverished areas: high costs and heavy resource usage. This thesis proposes a novel phacoemulsification system is based on the low-cost, off-shelf laser diodes that implement light-based phacoemulsification of cataracts. The system is tested for viability over two methods: finite element analysis and experimentation on various phantoms. Simulations on the device have occurred on the COMSOL …

Bayesian Multivariate Joint Modeling For Skewed-Longitudinal And Time-To-Event Data, Lan Xu

USF Tampa Graduate Theses and Dissertations

In epidemiologic and clinical studies, a relatively large number of biomarkers are repeatedly measured in patients over time, often associated with data on epidemiologic and clinical interest events. So, much attention is focused on developing the specific patterns of the longitudinal measurements, and the associations between those patterns and the time to a certain event, such as heart attack, diagnose of disease, time to transplantation, or death. In the last two decades, the research into joint modeling of longitudinal and time-to-event data has received a tremendous amount of attention.

Numerous researchers have proposed joint modeling approaches for a single longitudinal …

Saudi Teachers’ Perspectives On Implementing Evidence-Based Practices Specifically Designed For Students With Autism Spectrum Disorder, Ahmad Saad Alghamdi

USF Tampa Graduate Theses and Dissertations

The concept of evidence-based practices (EBP) for students with autism refers to a set of practices that were established based on research evidence that proved their effectiveness in helping improve functional and academic skills for children who have autism (Wong et al., 2015). Vision 2030 is an ambitious plan by the Ministry of Education in the Kingdom of Saudi Arabia (KSA). The ministry has published a new executive framework for the reform of teacher preparation programs (Planning and Development Agency, 2018). This reform is aimed at improving the quality of special educators, through the modernization of teacher preparation programs by …

Leaf Cutter Ant Nest Soil Cement Stabilized Earthen Bricks: Materials And Methods For Engineering Field Applications, Faith Malay

USF Tampa Graduate Theses and Dissertations

As climate change threatens vulnerable tropical climates and development outpaces resource availability, the need for diverse construction material grows. The United Nations Sustainable Development Goals aim to reduce poverty in all forms. Diversifying construction material works toward reducing indoor air pollution, the eradication of houselessness, encouraging diversification in industry, and supporting small scale industrialization. In the Province of Bocas del Toro in Panama, there is a tenuous connection to the greater industries in the country due to location and existing infrastructure.This research presents a literature review of the historical approaches to the construction and chemistry of cement stabilized earth bricks, …

Site Suitability Modeling In The Sand Pine Scrub Of The Ocala National Forest, Jelane M. Wallace

USF Tampa Graduate Theses and Dissertations

Central Florida’s Ocala National Forest is the largest remnant of the unique-to-the-region Sand Pine Scrub ecosystem. This ecosystem exhibits a surprising wealth of biodiversity despite what may be characterized as barren, difficult, dry, pyrogenic conditions. Significant prehistoric sites exist throughout the forest, even in the Sand Pine Scrub; however, most are on the margins and few systematic surveys penetrated this ecosystem, until now. I utilized GIS and these recently collected archaeological survey data, in conjunction with other environmental, geological, or historical data in GIS format, to model prehistoric settlement and land use patterns. This model attempts to address questions of …

Fire Severity Effects On Herpetofaunal Diversity In Florida Scrub Communities Of The Lake Wales Ridge, Michelle Lindsay

USF Tampa Graduate Theses and Dissertations

Fires, either natural or prescribed, are essential for conserving pyrogenic ecosystems; however, climate change is predicted to increase fire severity that could negatively impact species diversity. Reptile and amphibian species may be particularly at risk given they are ectothermic species. The objective of this study was to better understand the impacts of fire severity on the herpetofaunal communities of the Florida scrub habitats, which is an imperiled ecosystem that sustains over two-thirds of listed reptiles and amphibians in Florida. We conducted a field-based study to test taxonomic and functional herpetofaunal diversity differences across four varying fire severities: unburned, low, medium …

A Method For Compact Representation Of Heterogenous And Multivariate Time Series For Robust Classification And Visualization, Alla Abdella

USF Tampa Graduate Theses and Dissertations

Processing multivariate sensory time-series with variable lengths is a challenging problem across different application domains due to the naturally complex, high-dimensional, and often non-stationary nature of the data. There are many practical examples of this in the industry particularly for the applications of sensor networks in monitoring production or distribution of goods around the globe. This thesis tackles the specific problem of time-series data representation in how we can better summarize and visualize multi-variate time series data coming from numerous sources in a sensor network distributed across a wide range of application scenarios. On one hand, we think the analysis …

The Role Of Antecedent Music In The Running Routines Of Experienced Runners, Jennifer L. Cook

USF Tampa Graduate Theses and Dissertations

Promoting running as an accessible and cost-effective form of exercise is important because persistent runners have a 29 to 50% lower risk of cardiovascular mortality compared to those who never run (Lee et al., 2014). Although there is a body of research on music and running, the results have been inconclusive across the literature. This may be due to a reliance on self-report measures and the averaging of results employed with group designs. To address these issues, the current study measured performance (pace) as the primary dependent variable in a series of within-subject designs. First, an online cross-sectional survey (Study …

The Early Medieval Transition: Diet Reconstruction, Mobility, And Culture Contact In The Ravenna Countryside, Northern Italy, Anastasia Temkina

USF Tampa Graduate Theses and Dissertations

This research project evaluates the effects of increased mobility and culture contact on dietary practices, and on dietary variation among people buried at two northern Italian sites, Chiunsano di Ficarolo and Chiesazza di Ficarolo, located near the ancient Roman capital of Ravenna and dating 4th to 7th century CE. The Early Medieval period was a time of change, political instability, migration and invasion of the “barbarian” tribes, and diet was not unaffected. In particular, it is hypothesized that a new staple crop, millet, was introduced and that meat consumption had increased. The goal of this research is to use stable …

Archaeology And Seasonality Of Stock Island (8mo2), A Glades-Tradition Village On Key West, Ryan M. Harke

USF Tampa Graduate Theses and Dissertations

Later Glades-period cultures (ca. 500–1760 CE) of south Florida and the Florida Keys are understudied and thus poorly understood, especially those that pre-date the arrival of Spaniards to the New World. Recent archaeological models of their sociopolitical organization suggest that by the Glades I-II transition (750/800 CE), south Florida peoples were organized into what appear to be regional population centers (e.g., Pineland and Mound Key, Granada, Turner River) and smaller hinterland towns in the Everglades (e.g., Cane Patch, Bear Lake) and the Florida Keys (e.g., Stock Island, Clupper Site). Smaller towns are hypothesized to be sedentary, heterarchically-organized, simple chiefdoms from …

A Self-Study Of Need-Supportive Teaching In Elementary Art, Craig Jacobowitz

USF Tampa Graduate Theses and Dissertations

I present a self-study of my need-supportive teaching practice. I have long been curious about what it is about my teaching, what I say and do, that motivates students to work harder on their artwork and persevere when they become mired in difficulties. Need-supportive teaching addresses students' basic psychological needs within the classroom setting through high-quality teacher/student relationships and structure. Need-supportive teaching, motivation, and academic resilience are areas I made an extensive study of, as presented in chapter two. In March of 2020, the educational landscape in the United States was altered by the arrival of the unprecedented and novel …

Cognitive Ability And Ambivalence Toward Alcohol: An Examination Of Working Memory Capacity’S Influence On Drinking Behavior, Emily T. Noyes

USF Tampa Graduate Theses and Dissertations

Research stemming from dual-processing theories suggest that working memory capacity may have an important role in the ability to inhibit automatic tendencies when there is the motivation to do so (Barrett, Tugade, & Engle, 2004). Ambivalence, the simultaneous desire to engage in (approach motivation) and inhibit (avoidance motivation), often occurs with problematic behaviors like alcohol abuse. The current study sought to determine whether individual differences in working memory capacity moderate the relationship between approach, avoidance and subsequent drinking behavior in a clinical sample. A total of 66 individuals with alcohol use disorder (AUD) participated in a baseline assessment of working …

Factors Controlling Circulation At The Terminus Of A Shallow Seagrass-Dominated Estuary, Fort Desoto Bay, West-Central Florida, Charles Gray Vickery

USF Tampa Graduate Theses and Dissertations

Fort DeSoto Bay is a shallow seagrass-dominated estuary located landward of Mullet Key that is home to the most extensive seagrass beds in all of Tampa Bay. During a period of development in the 1950s and early 1960s, the bay underwent extensive physical modifications, including the construction of earthen causeways and dredged channels, which altered the bathymetry of the seafloor and changed natural circulation patterns. The stagnation of water in the southern bay and increased estuarine residence time led to elevated summer temperatures, reduced dissolved oxygen levels, and die-offs of seagrasses. Two 40-foot circulation bridges were installed on each causeway …

The Science Of Guessing: Critiquing Ancestral Estimation Through Computer Generated Statistical Analysis Within Forensic Anthropology In A Real-World Setting, Christopher J. Turner

USF Tampa Graduate Theses and Dissertations

Research on current methods of ancestral estimation must reflect on biological heritage to aid in human identification. Using modern craniometrics methods, how do individuals with a varied biological history affect ancestral estimation? Today, the most used and reliable methods for craniometrics analysis for ancestral estimation in forensic anthropology are computer programs. Two programs are analyzed in this study, Fordisc 3.0 and 3D-ID. The analysis of these computer programs goes beyond the controlled environment provided by an osteological collection. These remains of individuals were unidentified, only to be identified later, through academic research, police work, and public outreach. The selection of …

The Feasibility Of 18f-Fdg Labeled Rbc And Pet/Ct Imaging Blood Perfusion And Vascular Dysfunction Measurements In Pre-Clinical Research, Shaowei Wang

USF Tampa Graduate Theses and Dissertations

Heart disease, dementia, and stroke are significant public health issues and leading causes of mortality and morbidity worldwide. Abnormalities in blood perfusion and vascular structures are seen in varieties of pathologies, including cerebrovascular diseases like stroke and dementia, hypertension, cardiovascular disease like atherosclerosis, diabetes, peripheral artery disease, and vasculitis, and tumor angiogenesis. Imaging perfusion/vascular abnormalities in these patients often plays a vital role in clinical diagnosis and treatment decisions.

Vascular diseases typically present as macrovascular or microvascular diseases. Microvascular disease (MVD) refers to vascular disease primarily affecting the arterioles, venules, and capillaries in various organs. Furthermore, MVD is widely found …

Chorine Conversion: Biological And Water Quality Impact On Activated Carbon Block Point Of Use Filters, Horace S. Jakpa

USF Tampa Graduate Theses and Dissertations

Point-of-use (POU) activated carbon (AC) filters are ubiquitous in many U.S. households. AC can reduce concentrations of lead, other heavy metals, and mitigate taste and odor issues. However, AC filters also remove residual disinfectants, thus allowing for the proliferation of microbes in the filter. In chloraminated systems, this can lead to localized, filter-induced nitrification. Most notably, high nitrite and nitrate in drinking water can cause methemoglobinemia (blue baby syndrome) in children under the age of three, raising public health concerns.

As a control measure for nitrification within distribution systems, utilities practice periodic, short-term secondary disinfectant switches from chloramine to free …

Health Risk Assessment Of Local Populations Ingesting Water With Naturally Occurring Arsenic And Fecal Related Contaminants In Lake Atitlan, Guatemala, Marisol Alvarez

USF Tampa Graduate Theses and Dissertations

Lake Atitlan, Guatemala acts as a sink for wastewater discharge and as a source of drinking water. The populations living in the lake basin are primarily indigenous with a large percentage (70%) living in poverty. Wastewater effluent entering the lake poses an immediate health risk to those using it as a drinking-water source. Additionally, the lake is surrounded by three volcanoes and the water has concentrations of naturally occurring arsenic above the recommended reference level of 10 µg/L. Arsenic is acutely poisonous at high concentrations and has carcinogenic and neurological effects when chronically exposed at low concentrations.

The goal of …

Circrev1 Expression In Triple-Negative Breast Cancer, Meagan P. Horton

USF Tampa Graduate Theses and Dissertations

Triple-negative breast cancer (TNBC) comprises only 24% of breast cancer cases, yet is the second leading cause of cancer mortality in women due to its aggressive nature (1). This increase in mortality is due to the lack of receptors for three targetable growth factors (HER2, progesterone, and estrogen receptors). Our previous studies have indicated that these cancers are highly dysregulated in respect to alternative splicing. Hence, we undertook a study aimed at identifying circular RNAs (circRNAs) generated from back-splicing events which were dysregulated in TNBC. We have identified a novel circRNA transcript, circular REV1 (circREV1), which is upregulated in our …

Framing #Metoo Movement In China A Content Analysis Of China Women’S News Coverage, Wenminzi Wu

USF Tampa Graduate Theses and Dissertations

Grounded in the framing theory, this article examined a differential study by content analysis. After the #MeToo movement rapidly trended in at least 85 countries including China, many people’s life and mind have been changed. The purpose of this study was to explore the frames used in the news coverage in China Women’s News referring to sexual harassment/assault and to examine how far the sense of gender equality has been changed in China especially after the #MeToo movement began. The sample came from the online database of a total of 546 relevant coverage from 2014 to 2020 in China Women’s …