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A Comparative Study Of Artificial Neural Networks And Info Fuzzy Networks On Their Use In Software Testing, Deepam Agarwal
A Comparative Study Of Artificial Neural Networks And Info Fuzzy Networks On Their Use In Software Testing, Deepam Agarwal
USF Tampa Graduate Theses and Dissertations
It is very important that the software being delivered to the user is reliable and fault free. This makes software testing one of the most important phases in the software development life cycle. The problem being faced by everyone is the time it takes to test the software, which is normally huge. An important part of the software testing process is running and evaluating test scenarios. The objective of this part is to evaluate how well the application under test conforms to its specifications. One of the ways to achieve this is to generate the test cases and make use …
A Stable Isotopic Examination Of Particulate Organic Matter During Karenia Brevis Blooms On The Central West Florida Shelf: Hints At Nitrogen Sources In Oligotrophic Waters, Julie Ann Havens
USF Tampa Graduate Theses and Dissertations
Blooms of the red tide dinoflagellate Karenia brevis occur annually on the west Florida shelf. In the late summer/early fall months, background concentrations increase from 103 cells L-1 to excesses of 106 cells L-1. Blooms are most common between Tampa Bay and Charlotte Harbor, and may be maintained for months. The region’s hydrography may play a role in the initiation, maintenance and termination of blooms.
The west Florida shelf is depauperate in inorganic nutrients. Inorganic nitrogen and phosphorus rarely exceed the limits of detection, whereas dissolved organic nitrogen is often present at concentrations of 15 …
Preparation And Characterization Of Ribonucleic Acid (Rna)/Inorganic Materials Interfaces Using Photoemission Spectroscopy, Brian, Doran
Preparation And Characterization Of Ribonucleic Acid (Rna)/Inorganic Materials Interfaces Using Photoemission Spectroscopy, Brian, Doran
USF Tampa Graduate Theses and Dissertations
The objective of this master s thesis is the preparation and characterization of ribonucleic acid (RNA)/inorganic material interfaces by electrospray deposition and photoemission spectroscopy. This was done through investigation of the chemical and electronic structure of the surface of Highly Ordered Pyrolytic Graphite (HOPG) and gold before and after multiple steps of RNA thin film deposition by electrospray. Great interest has been shown by researchers into RNA due to its self-assembling ability. A series of experiments was conducted depositing RNA Poly adenosine, RNA Poly cytidine, and (for control purposes) DI water on HOPG. RNA Poly adenosine was also deposited on …
Florida Bay Microalgae Blooms: Physiological Characteristics And Competitive Strategies Of Bloom Forming Cyanobacteria And Diatoms Of Florida Bay, Ralph William, Richardson
Florida Bay Microalgae Blooms: Physiological Characteristics And Competitive Strategies Of Bloom Forming Cyanobacteria And Diatoms Of Florida Bay, Ralph William, Richardson
USF Tampa Graduate Theses and Dissertations
Areally expansive, persistent and recurring blooms frequently dominated by cyanobacteria have developed primarily in the north-central region of Florida Bay since approximately 1991. This part of the bay has a history of the following: periodic hypersalinity, high sediment-derived turbidity, P limitation, N limitation, light limitation and long water residence time. Clonal isolates of selected dominant bloom species of cyanobacteria (Synechococcus cf. elongatus and Synechocystis sp.) and diatoms (Chaetoceros cf. salsugineus and Thalassiosira cf. oceanica) from Florida Bay were examined in an effort to explain their relative dominance of the phytoplankton community.
The following physiological characteristics and nutrient …
Molecular Mechanism Of Vitamin D Action And Its Implications In Ovarian Cancer Prevention And Therapy, Feng Jiang
Molecular Mechanism Of Vitamin D Action And Its Implications In Ovarian Cancer Prevention And Therapy, Feng Jiang
USF Tampa Graduate Theses and Dissertations
1,25-dihydroxyvitamin D3 (1,25VD), the active form of vitamin D (VD), suppresses the growth of numerous human cancer cell lines by inhibiting cell cycle progression and inducing cell death. Genes that mediate each of these activities remain largely unidentified and there are no preclinical data for 1,25VD analogues in ovarian cancer (OCa). We hypothesize that 1,25VD and its analogues inhibit the development of OCa. In this study, we demonstrated, (a) 1,25VD causes cell cycle arrest at the G1/S and G2/M transition and induces apoptosis in OCa cells. (b) We also found that gadd45 is one of primary target genes for 1,25VD-mediated …
Crystal Engineering Of Metal-Carboxylate Based Coordination Polymers, Jianjiang Lu
Crystal Engineering Of Metal-Carboxylate Based Coordination Polymers, Jianjiang Lu
USF Tampa Graduate Theses and Dissertations
This dissertation endeavors to delineate practical paradigms for crystal engineering based upon the understanding of supramolecular chemistry and self-assembly, i.e. the design and synthesis of novel functional crystalline materials.
Two basic metal-organic building units, Zn(RCO2)2(py)2 and (L2)M2(RCO2)4 (M = Zn, Cu), as well as nano-scaled secondary building units (nSBUs) that are constructed from Cu2(RCO2)4 are researched and discussed. Design strategies have been developed to propagate these metal-organic synthons into predictable coordination polymer networks. A series of crystal structures, as well as their syntheses …
Auditory Sensitivity Of Sergeant Majors (Abudefduf Saxatilis) From Post-Settlement Juvenile To Adult, Sarah A. Egner
Auditory Sensitivity Of Sergeant Majors (Abudefduf Saxatilis) From Post-Settlement Juvenile To Adult, Sarah A. Egner
USF Tampa Graduate Theses and Dissertations
There is much evidence supporting the idea that pelagic larvae of coral reef fishes are active participants in their dispersal and return to a reef, however, the mechanisms used to navigate are still uncertain. It has been proposed that sensory cues, such as hearing, play a role. Sound is a potentially important cue for organisms in marine environments, especially in noisy environments like coral reefs. Sensory organs, including otolithic organs, of most coral reef fish form within the first few days of life. The auditory brainstem response (ABR) technique was used to measure hearing on a wide size range of …
Biological Indicators Of Wetland Health: Comparing Qualitative And Quantitative Vegetation Measures With Anuran Measures, Shannon M. Gonzalez
Biological Indicators Of Wetland Health: Comparing Qualitative And Quantitative Vegetation Measures With Anuran Measures, Shannon M. Gonzalez
USF Tampa Graduate Theses and Dissertations
Understanding wetland responses to human perturbations is essential to the effective management of Florida's surface and ground water resources. Southwest Florida Water Management District (SWFWMD) Rules (Chapter 40D-2.301(c) FAC) prohibit adverse environmental effects to wetlands, fish and wildlife caused by groundwater withdrawal. Numerous studies have documented the responses of biological attributes across taxa and regions to human disturbance. Biological assessment can provide information about ecological condition. Based on long-term monitoring conducted by the SWFWMD, the anthropogenic changes observed on the Starkey Wellfield are attributed to groundwater withdrawal.
Biological indicators are species, species assemblages, or communities whose presence, abundance, and condition …
Effects Of Gloves And Visual Acuity On Dexterity, Mehdi Pourmoghani
Effects Of Gloves And Visual Acuity On Dexterity, Mehdi Pourmoghani
USF Tampa Graduate Theses and Dissertations
Work in many environments with chemical or biological agents requires the use of personal protective equipment such as gloves and respirators. It is well established that glove thickness affects finger dexterity. There is further evidence that visual constraints (e.g., visual acuity) and gender may also impede finger dexterity. Therefore, the personal protection may place a barrier to the agent, but performance or productivity will decrease. The purpose of this study is to examine the potential effects of gloves and visual acuity as well as gender and first order interactions on task performance using standard dexterity tests.
Five men and five …
Near-Death Experiences, Religion, And Life After Death, Holly Wallace
Near-Death Experiences, Religion, And Life After Death, Holly Wallace
USF Tampa Graduate Theses and Dissertations
This thesis investigates current scientific literature on near-death experiences (NDE), focusing on two common types of models used to explain the NDE: biological models and spiritual models. Four biological models and two spiritual models are presented and evaluated. During the evaluation, particular emphasis is placed on how these models accommodate veridical evidence. I found that, of the biological models, only one could account for veridical evidence, whereas both of the spiritual models provided explanations of these strange occurrences. From this conclusion, the implications of NDE research on human survival are explored.
Tracing The Source Of The Elephant And Hippopotamus Ivory From The 14th Century B.C. Uluburun Shipwreck: The Archaeological, Historical, And Isotopic Evidence, Kathryn Anne Lafrenz
Tracing The Source Of The Elephant And Hippopotamus Ivory From The 14th Century B.C. Uluburun Shipwreck: The Archaeological, Historical, And Isotopic Evidence, Kathryn Anne Lafrenz
USF Tampa Graduate Theses and Dissertations
The aim of this study is to establish the provenance of the elephant and hippopotamus ivory recovered from the 14th century B.C. Uluburun shipwreck in order to reconstruct the trade mechanisms and associated social relationships (e.g. diplomacy) operating in the eastern Mediterranean during the Late Bronze Age (LBA). Elephant ivory came either from Northeastern Libya, Southeastern Sudan via Egypt or northwestern Syria during this period. Hippopotamus ivory likewise was obtained from Syria, Palestine, or Egypt.
The Uluburun's cargo is reconstructed by the excavator, George Bass, as "royal," and primarily originates from Cyprus and Syro-Palestine. Indeed, LBA trade is largely understood …
Symbolic Regression Of Thermo-Physical Model Using Genetic Programming, Ying Zhang
Symbolic Regression Of Thermo-Physical Model Using Genetic Programming, Ying Zhang
USF Tampa Graduate Theses and Dissertations
The symbolic regression problem is to find a function, in symbolic form, that fits a given data set. Symbolic regression provides a means for function identification. This research describes an adaptive hybrid system for symbolic function identification of thermo-physical model that combines the genetic programming and a modified Marquardt nonlinear regression algorithm.
Genetic Programming (GP) system can extract knowledge from the data in the form of symbolic expressions, i.e. a tree structure, that are used to model and derive equation of state, mixing rules and phase behavior from the experimental data (properties estimation).
During the automatic evolution process of GP, …
Educational Choices Of Undergraduate Women In Public Relations: A Quantitative Study, Moira K. Davis
Educational Choices Of Undergraduate Women In Public Relations: A Quantitative Study, Moira K. Davis
USF Tampa Graduate Theses and Dissertations
Public relations is one of the fastest growing majors chosen by college students throughout the United States, and most of these students are women. This thesis attempts to define the important role that women play in the practice of public relations and to determine why female students choose this major as their desired field of study more often then men. There is a need for such a study because in 1996 women accounted for only 37 percent of managers in marketing, advertising, and public relations, although they comprised 62 percent of all public relations specialists in this country. This research …
The Effects Of Seasonal Change On Copepods And Euphausiids Off The Western Antarctic Peninsula: Results From Biochemical Assays And Respiration Studies, Joël Laurent Bellucci
The Effects Of Seasonal Change On Copepods And Euphausiids Off The Western Antarctic Peninsula: Results From Biochemical Assays And Respiration Studies, Joël Laurent Bellucci
USF Tampa Graduate Theses and Dissertations
We compared four metabolic indicators of nutritional state: citrate synthase activity (CS), malate dehydrogenase activity (MDH), lactate dehydrogenase activity (LDH) and percent body protein to each other and to respiration measurements. These comparisons were made for four species of copepods (Calanoides acutus, Metridia gerlachei, Paraeuchaeta antarctica and another form of Paraeuchaeta that was unidentifiable to species due to its early life stage), three species of Euphausids (Euphausia crystallorophias, Euphausia triacantha, Euphausia superba (including both F6-furcilial and adult stages)) and Thysonessa macrura which were collected off the Western Antarctic Peninsula (WAP) during Spring/Summer and Fall/Winter. …
Structural Diversity In Metal-Organic Nanoscale Supramolecular Architectures, Heba Abourahma
Structural Diversity In Metal-Organic Nanoscale Supramolecular Architectures, Heba Abourahma
USF Tampa Graduate Theses and Dissertations
Supramolecular synthesis has gained much attention in recent years. Such an approach to synthesis represents an attractive alternative to traditional, multi-step synthesis, especially for making complex, nanoscopic structures. Of particular interest, in the context of this work, is the use of metal-organic interactions to direct the self-assembly of nanoscopic architectures. These interactions are highly directional, relatively "strong" (compared to other supramolecular interactions) and kinetically labile, which allows for "self-correction" and in turn the production, often in high yield, of defect-free products. This also means that a number of related, yet structurally diverse products (supramolecular isomers) could be isolated.
The work …
Real Time Digital Signal Processing Adaptive Filters For Correlated Noise Reduction In Ring Laser Gyro Inertial Systems, David A. Doheny
Real Time Digital Signal Processing Adaptive Filters For Correlated Noise Reduction In Ring Laser Gyro Inertial Systems, David A. Doheny
USF Tampa Graduate Theses and Dissertations
Existing opportunities in advanced interceptor, satellite guidance and aircraft navigation technologies, requiring higher signal processing speeds and lower noise environments, are demanding Ring Laser Gyro (RLG) based Inertial Systems to reduce initialization and operational data latency as well as correlated noise magnitudes. Existing signal processing algorithms are often less than optimal when considering these requirements. Advancements in micro-electronic processes have made Application Specific Integrated Circuits (ASIC) a fundamental building block for system implementation when considering higher-level signal processing algorithms.
Research of real time adaptive signal processing algorithms embedded in ASICs for use in RLG based inertial systems will help to …
A Comparison Of Tone Burst Auditory Brainstem Response (Abr) Latencies Elicited With And Without Notched Noise Masking, Rachele M. Orsini
A Comparison Of Tone Burst Auditory Brainstem Response (Abr) Latencies Elicited With And Without Notched Noise Masking, Rachele M. Orsini
USF Tampa Graduate Theses and Dissertations
Objective: The air conduction click is currently the most widespread stimulus used to estimate the pure tone audiogram as part of auditory brainstem response (ABR) testing. The click is characterized by its broad spectral content and rapid onset, which may not make it the best choice for frequency specificity. An alternative stimulus is a short duration tone burst stimuli with notched noise masking. When obtaining an ABR using standard unmasked tone bursts, the brief stimulus onset may cause spectral splatter producing response contributions from unwanted regions of the cochlea; thus reducing the frequency specificity of the ABR. Notched noise masking …
Comparative Analysis Of Serologic Assays For The Detection Of Antibodies To Eastern Equine Encephalomyelitis Virus In Sentinel Chickens, Christy L. Voakes
Comparative Analysis Of Serologic Assays For The Detection Of Antibodies To Eastern Equine Encephalomyelitis Virus In Sentinel Chickens, Christy L. Voakes
USF Tampa Graduate Theses and Dissertations
Florida's mild climate supports year round enzootic transmission of arthropod-borne (arbo) viruses, such as St. Louis Encephalitis virus (SLEV), West Nile virus (WNV), and Eastern Equine Encephalomyelitis virus (EEEV). First isolated in 1960 from two Florida blue jays, Highlands J virus (HJV) is endemic to the state and vectored by the same mosquitoes as EEEV (Henderson et al, 1962). EEEV and HJV are both alphaviruses, but HJV is not pathogenic to humans, occasionally causes encephalitis in horses, and is a recognized pathogen in some bird species (turkeys, emus, etc) (Cilnis et al, 1996).
The Florida Sentinel Chicken Arboviral Surveillance …
The Politics Of Being An Egg “Donor” And Shifting Notions Of Reproductive Freedom, Elizabeth A. Dedrick
The Politics Of Being An Egg “Donor” And Shifting Notions Of Reproductive Freedom, Elizabeth A. Dedrick
USF Tampa Graduate Theses and Dissertations
As an Assisted Reproductive Technology (ART) that has been available for over twenty years, the transfer of healthy eggs from a presumably fertile woman into the womb of a woman diagnosed as infertile has become a common part of the landscape of human reproduction in the United States. Yet the general societal acceptance of this practice commonly known as "egg donation" oversimplifies the complex medical, ethical, and societal issues ignited by its use. In light of the limited critical discussions presently occurring about egg transfer, I will interrogate some of the silences and more ambiguous issues invoked by its practice. …
From Just War To Just Peace: Re-Visioning Just War Theory From A Feminist Perspective, Naomi Malone
From Just War To Just Peace: Re-Visioning Just War Theory From A Feminist Perspective, Naomi Malone
USF Tampa Graduate Theses and Dissertations
This paper studies the history of just war theory and critiques it from various feminist perspectives. Using a definition of war as inseparable from the system within which it is embedded, the paper contends that just war theory has been incorporated into the realist paradigm that predominates current political thought, making it susceptible to manipulation. Most importantly, this usurpation has shifted just war theory's focus from jus ad bellum to jus in bello considerations, seriously weakening its deterrent effects on war. The paper proposes its replacement with a just peace theory, discussing several existing frameworks and explaining the important part …
Concerning Theories Of Personal Identity, Patrick, Bailey
Concerning Theories Of Personal Identity, Patrick, Bailey
USF Tampa Graduate Theses and Dissertations
The purpose of this thesis is to provide a brief examination of the historical accounts of philosophical theories of personal identity and show the influence that each has had on the development of contemporary theories. In doing so, the thesis explores the problems associated with these theories, attempting to establish a meta-theory (i.e. a theory about theories) of personal identity. What is demonstrated is that the fundamental problems of personal identity arise from issues related to the use of language, as well as assumptions involving the concept of personhood.
By demonstrating that our understanding of personhood is relative to frameworks …
A Comparison Of Exhaled Breath Nitric Oxide Between Old And Young Individuals, Robert L. Gordon
A Comparison Of Exhaled Breath Nitric Oxide Between Old And Young Individuals, Robert L. Gordon
USF Tampa Graduate Theses and Dissertations
BACKGROUND: Older individuals suffer from higher rates of pulmonary infections than younger individuals. In addition, older individuals have increased morbidity and mortality due to pulmonary infections when compared to younger individuals. The physiological and immunological reasons for these aforementioned differences are not clear. Recently, non-invasive markers of the lung's physiologic and immunologic status have been recognized. This study employs one of these non-invasive markers, exhaled nitric oxide, in an attempt to determine how the airways may change with age, predisposing older individuals to pulmonary diseases and poorer outcomes as compared to younger individuals.
METHODS: Exhaled nitric oxide measurements were obtained …
Ammonia Flux At The Air/Water Interface Of Tampa Bay, Constance Anne Mizak
Ammonia Flux At The Air/Water Interface Of Tampa Bay, Constance Anne Mizak
USF Tampa Graduate Theses and Dissertations
An ammonia emissions inventory discovered that 90% of the ammonia emitted from Pinellas, Hillsborough, and Polk counties, originated from the latter two counties. This finding is significant and suggests that a substantial portion of the ammonia deposited to Tampa Bay is transported with easterly air masses.
Ammonia and ammonium concentrations at the coastal Gandy Bridge site were seasonally and diurnally consistent, but the rural Sydney site showed greater variability. It was determined that wind direction was the most influential parameter affecting the Gandy Bridge site, which supports the hypothesis that an advection of ammonia from the east is a major …
Detection And Quantification Of Karenia Brevis Blooms On The West Florida Shelf From Remotely Sensed Ocean Color Imagery, Jennifer P. Cannizzaro
Detection And Quantification Of Karenia Brevis Blooms On The West Florida Shelf From Remotely Sensed Ocean Color Imagery, Jennifer P. Cannizzaro
USF Tampa Graduate Theses and Dissertations
Karenia brevis, a toxic dinoflagellate species that blooms regularly in the Gulf of Mexico, frequently causes widespread ecological and economic damage and can pose a serious threat to human health. Satellite-based ocean color imagery may provide a means for detecting and monitoring blooms, providing early alerts to coastal communities. However, a technique for discriminating K. brevis from other chlorophyll-containing algae is required. Between 1999 and 2001, a large bio-optical data set consisting of spectral measurements of remote-sensing reflectance (Rrs(lambda)), absorption (a(lambda)), and backscattering (bb(lambda)) along with chlorophyll a concentrations was collected on the central west Florida shelf (WFS) as …
Supramolecular Metal-Organic And Organic Materials, Elisabeth Rather
Supramolecular Metal-Organic And Organic Materials, Elisabeth Rather
USF Tampa Graduate Theses and Dissertations
The rational design of functional solids based upon the development of strategies for controlling intermolecular interactions and structural arrangement of simple molecular building units, represents a salient feature in the context of supramolecular chemistry and crystal engineering. Consideration of chemical functionality, geometrical capability and knowledge of the interplay between two or more sets of supramolecular interactions specific of preselected chemical components will facilitate further extension of crystal engineering towards the construction of supramolecular materials possessing valuable properties.
Calixarenes represent excellent building blocks for the design of solid-state architectures, in particular calix-4-arenes crystallize easily and the introduction of a wide range …
Phase Imaging Digital Holography For Biological Microscopy, Daniel Parshall
Phase Imaging Digital Holography For Biological Microscopy, Daniel Parshall
USF Tampa Graduate Theses and Dissertations
We apply the techniques of digital holography to obtain microscopic 3D images of biological cells. Both the amplitude and phase images are obtained from a single hologram, with approximately 1 [mu]m lateral and 10 nm longitudinal resolution. The results are combined with previous experiments resolving the 2 [pi] ambiguity to produce continuous phase images of the samples.
Distribution, Metabolism And Trophic Ecology Of The Antarctic Cydippid Ctenophore, Callianira Antarctica, West Of The Antarctic Peninsula, Kerri M. Scolardi
Distribution, Metabolism And Trophic Ecology Of The Antarctic Cydippid Ctenophore, Callianira Antarctica, West Of The Antarctic Peninsula, Kerri M. Scolardi
USF Tampa Graduate Theses and Dissertations
The distribution, abundance, chemical composition, metabolism, and feeding ecology of the tentaculate ctenophore, Callianira antarctica (Chun 1897), were investigated during austral winter 2001and autumn & winter 2002, in the vicinity of Marguerite Bay west of the Antarctic Peninsula. Callianira antarctica had a widespread distribution during autumn and winter, and variable abundance (0.02 to 2.6 ind. m-2) during winter 2001 associated with specific circulation features. Size frequency distributions for autumn and winter suggest that more than half of the C. antarctica population may have experienced 'degrowth' during winter due to low food availability. Callianira antarctica is a fairly robust ctenophore with …
Risk Factors For Pediatric Community Acquired Methicillin Resistant Staphylococcus Aureus, Melissa Gail Kessler
Risk Factors For Pediatric Community Acquired Methicillin Resistant Staphylococcus Aureus, Melissa Gail Kessler
USF Tampa Graduate Theses and Dissertations
Methicillin-Resistant Staphylococcus aureus (MRSA) began as a nosocomial infection due to overuse of antibiotics. Several previous studies have reported an increase in this infection in adult patients who have not been hospitalized. It has also been reported that there is an increase in MRSA in children. Some of these children became infected even though they were not at high risk for the infection. After approval from the All Children's Hospital Institutional Review Board (IRB), a cross sectional study was conducted with pediatric admissions and pediatric emergency room visits to determine the characteristics of Methicillin-Sensitive Staphylococcus aureus and MRSA. During this …
Synthesis And Characterization Of Potential Drug Delivery Systems Using Nonionic Surfactant “Niosome”, Sukit Leekumjorn
Synthesis And Characterization Of Potential Drug Delivery Systems Using Nonionic Surfactant “Niosome”, Sukit Leekumjorn
USF Tampa Graduate Theses and Dissertations
Niosomes are synthetic microscopic vesicles consisting of an aqueous core enclosed in a bilayer consisting of cholesterol and one or more nonionic surfactants. They are made of biocompatible, biodegradable, non-toxic, non-immunogenic and non-carcinogenic agents which form closed spherical structures (self assembly vesicles) upon hydration. With high resistance to hydrolytic degradation, niosomes are capable of entrapping many kinds of soluble drugs while exhibiting greater vesicle stability and longer shelf life.
In this work, a potential drug delivery system has been designed, synthesized and characterized. For the synthesis of niosomes, a hydration process was developed with varying design parameters such as mass …
Synthesis Of Nanoscale Structures In Single Crystal Silicon Carbide By Electron Beam Lithography, Jay A. Bieber
Synthesis Of Nanoscale Structures In Single Crystal Silicon Carbide By Electron Beam Lithography, Jay A. Bieber
USF Tampa Graduate Theses and Dissertations
Nanostructures were formed on diced specimens of several silicon carbide polytypes and silicon using electron beam lithography. A general introduction to nanostructure synthesis and electron beam lithography,are presented. A scanning electron microscope was retrofitted with a commercially available electron beam lithography package and an electrostatic beam blanker to permit nanoscale lithography to be performed.
A process was first developed and optimized on silicon substrates to expose, poly-methyl-methacrylate (PMMA) resist with an electron beam to make nanoscale nickel masks for reactive ion etching. The masks consist of an array of nickel dots that range in size from 20 to 100 nm …