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Benzene Related Hematological Disorders: Evidence For A Threshold In Animals And Humans, James Mccluskey

USF Tampa Graduate Theses and Dissertations

Significant benzene exposure has historically been associated with the development of a host of hematological disorders in humans and animals. In particular, benzene is known to cause disturbances of the peripheral blood, aplastic anemia and cancer of the lymphohematopoietic system. In 1928, the first modern report of an association between cancer and benzene exposure was published. This case report was followed by additional reports from around the world. In most instances, ailments resulted from long term, high level exposure to benzene found in glues, and through accidental industrial spills. Throughout the 1960's and 1970's, case reports accumulated linking benzene exposure …

Stable Carbon Isotope Discrimination By Form Ic Rubisco From Rhodobacter Sphaeroides, Phaedra Thomas

USF Tampa Graduate Theses and Dissertations

Variations in the relative amounts of ¹²C and ¹³C in microbial biomass can be used to infer the pathway(s) autotrophs use to fix and assimilate dissolved inorganic carbon. Discrimination against ¹³C by the enzymes catalyzing autotrophic carbon fixation is a major factor dictating the stable carbon isotopic composition (δ¹³C = {[¹³C/¹²C_sample/¹³C/¹²C_standard] - 1} X 1000) of biomass. Six different forms of ribulose 1,5-bisphosphate carboxylase/oxygenase or RubisCO (IA, IB, IC, ID, II, and III), the carboxylase of the Calvin-Benson-Bassham cycle (CBB), are utilized by algae and autotrophic bacteria that rely on the CBB cycle for carbon fixation. …

Face To Face: English Uses And Understanding Of The Beard In Early Virginian Contacts, Jacqueline Colleen Barber

USF Tampa Graduate Theses and Dissertations

Many historians agree that categories of human division underwent a drastic change due to European New World encounters. The shift from religious divisions to ones based on ethnicity and skin color gradually developed in early modern Europe. Hence, before natives became "red," and Europeans "white" a period existed where the differences between these cultures were utilized in a variety of means to prove similarity and difference.

One element signifying difference during the early contact period was that of the beard. Hair as an identifier has a long history: through the middle ages, wildness was conveyed by hair and at times …

The Cumulative Effects Of Victimization, Community Violence, And Household Dysfunction On Depression And Suicide Ideation In A Cohort Of Adolescent Females, Katherine Best

USF Tampa Graduate Theses and Dissertations

Recent scholarly efforts have sought to examine the cumulative impact of deleterious adverse childhood exposures on various mental health outcomes. Lifetime prevalence rates for depressive disorders are approximately 20% among adolescents. Depression is ranked as the leading cause for disability and fourth leading contributor to the global burden of disease in the world.

The purpose of this study was to determine the cumulative impact of adolescent adverse experiences on outcomes of depression, suicide ideation, and overall mental distress in a cohort of 125 adolescent girls receiving public assistance. The adverse exposures studied were personal victimization, household dysfunction, and community violence …

Microarray Analysis Of Streptococcus Mutans And Actinomyces Viscosus In Homologous And Heterologous Cultures, Steven Andrew Horton

USF Tampa Graduate Theses and Dissertations

The oral pathogen Streptococcus mutans is a known etiological agent for dental root decay and coronal caries. It has been hypothesized, but not yet proven, that S. mutans expression of virulence genes in dental plaque may be influenced by its interaction with co-aggregating partners, notably Fusobacterium nucleatum and Actinomyces viscosus. Investigation of the suitability of mixed cultures of S. mutans with F. nucleatum versus S. mutans with A. viscosus proved that A. viscosus was a better target in the present laboratory setting. Furthermore, A. viscosus, a causative agent of mandible osteomyelitis and endocarditis, has been shown to have …

Systematic Analysis Of Structure-Function Relationships Of Conserved Sequence Motifs In The Nadh-Binding Lobe Of Cytochrome B5 Reductase, Glenn W. Roma

USF Tampa Graduate Theses and Dissertations

NADH:Cytochrome b₅ Reductase (cb5r) catalyzes the reduction of the ferric iron (Fe³⁺) atom of the heme cofactor found within cytochrome b₅ (cb5) by the reduction of the FAD cofactor of cb₅r from reducing equivalents of the physiological electron donor, reduced nicotinamide adenine dinucleotide (NADH). Cb5r is characterized by the presence of two domains necessary for proper enzyme function: a flavin-binding domain and a pyridine nucleotide-binding domain. Within these domains are highly conserved "motifs" necessary for the correct binding and orientation of both the NADH coenzyme and the FAD cofactor.

To address the importance …

The Channel For Gay America? A Cultural Criticism Of The Logo Channel’S Commercial Success On American Cable Television, Michael Johnson Jr.

USF Tampa Graduate Theses and Dissertations

Logo currently holds a self-described monopoly as the "Gay Channel for America." Logo stands alone as the single most concentrated national-level vehicle of LGBT (Lesbian, Gay, Bisexual and Transgendered) visibility in the post millennial television era. The Logo Channel has reaped financial rewards from its strategy as a business entity, as LGBT American television viewers embraced its presence as a signifier to America that gays and lesbians have finally "made it".

First, any claim to a monopoly deserves critical attention for its place in mainstream television, for its business practices, and for the power it holds in representing and targeting …

Directed Self-Assembly Of Gold Nanorods Using Surface Modification, David A. Walker

USF Tampa Graduate Theses and Dissertations

Metallic nanoparticles are unique materials for optical, electronic, catalytic, and sensing applications. Due to the vast flexibility in controlling the surface chemistry of these particles through functionalization there is a great deal of interest in using metallic nanoparticles as building blocks in the development of more complex nanostructures through the use of a 'bottom-up' approach. Using self assembly techniques, one can exploit spontaneous chemical interactions to build complex constructs on the nanometer scale.

Towards this end, gold nanorods have been synthesized and modified with various polymers, inorganic oxides and organic ligands to establish principles for self-assembly of these unique nanomaterials. …

Role Of Foxo Factors As The Nuclear Mediator For Pten-Ar Antagonism In Prostate Cancer Cells, Qiuping Ma

USF Tampa Graduate Theses and Dissertations

FoxO proteins are transcriptional factors acting downstream of the tumor suppressor PTEN. Their activity is negatively regulated by AKT-mediated phosphorylation. Our previous studies showed a mutual suppression between PTEN and the androgen receptor (AR) in regulating growth and apoptosis in prostate cancer (PCa) cells. We hypothesize that nuclear FoxO proteins are involved in mediating this mutual antagonism. In this dissertation, we report that PTEN inhibits AR activity through FoxO1 and provide evidence for the involvement of FoxO factors in the androgen-mediated suppression of PTEN-induced apoptosis. Our studies identify a novel mechanism for AR inhibition by FoxO1 and demonstrated the participation …

Structural Diversity In Metal-Organic Materials, Gregory J. Mcmanus

USF Tampa Graduate Theses and Dissertations

The interest in metal-organic materials namely, coordination polymers and metal-organic frameworks has risen dramatically over the past few years. To a certain extent this interest is a consequence of the realization chemists have discovered how to play a form of molecular Lego® in which metal cations or metal clusters represent the bricks (or nodes) and organic ligands such as 4,4-bipyridine (bipy) or benzene-dicarboxylate represents the glue (or spacers). The "node-and-spacer" approach to self-assembly can be invoked in such a manner that a plethora of infinite architectures and discrete polyhedra can be generated from geometric principles, some of which are unprecedented …

Gardens At Home, Gardens At School: Diet And Food Crop Diversity In Two Q'Eqchi' Communities In Southern Belize, Douglas Carl Reeser

USF Tampa Graduate Theses and Dissertations

A district-wide school garden project has been initiated in the Toledo District of Southern Belize in response to reported high rates of poverty and undernutrition. This paper will discuss research conducted in the summer of 2007 with Q'eqchi' Maya in the Toledo District to determine the effect of school gardens on household diet and gardening, the composition of the household diet, and the makeup of homegardens. Food frequency questionnaires and semi-structured interviews were conducted in two rural villages, one with a school garden program and one without. Various members of NGOs working on school garden projects in the district were …

Can Using One Trainer Solely To Deliver Prompts And Feedback During Role Plays Increase Correct Performance Of Parenting Skills In A Behavioral Parent Training Program?, Michael M. Cripe

USF Tampa Graduate Theses and Dissertations

Behavioral Parent Training refers to a broad range of instructional programs that teach parents and other caregivers ways to build and change behavioral repertoires of children. Most, if not all, such programs employ Behavioral Skills Training (modeling, prompting, role-play practice and feedback) to teach parenting skills. However, specific ways to use prompting during role plays have not been described in the behavioral parent training literature. The present study compared two methods of conducting role plays during parent training. A between group, pretest/posttest design was used to evaluate and compare the effect of using one versus two trainers on the role-play …

Molecular Mechanism Of Aurora-A Kinase In Human Oncogenesis, Lili He

USF Tampa Graduate Theses and Dissertations

Aurora-A is a mitotic kinase, which regulates cell cycle progression through modulating centrosome function. Aurora-A expression is frequently altered in human malignancies. The discrepancy between overexpression and amplification suggests that elevated Aurora-A level is likely to be regulated also by transcriptional and/or translational modifications. In this study, we have demonstrated: 1) transcriptional regulation of Aurora-A by E2F3; 2) feedback regulation between tumor suppressor CHFR and Aurora-A; 3) CNTD2 as a novel Aurora-A partner and oncogene to activate Aurora-A and induce transformation.

Aurora-A expression and activity are cell cycle regulated. The mechanism of Aurora-A upregulation at onset of mitosis is largely …

Investigating Environmental Change Due To Hypoxic Conditions On The Louisiana Continental Shelf: A Geochemical Approach, Marianne E. Dietz

USF Tampa Graduate Theses and Dissertations

The Louisiana (LA) shelf is chronically affected by seasonal hypoxia that has been shown to be spatially expanding and growing progressively more severe. Hypoxic conditions on the shelf have been closely linked to the large quantities of nutrients delivered to the Gulf of Mexico via the Mississippi River. Multiple geochemical proxies on a suite of sediment cores from the LA shelf provide a record of environmental change that parallels the onset of hypoxic conditions over the last century and prior to anthropogenic influences. The sedimentary record for the last century shows a shift from terrestrial to algal sources to the …

Enantioselective Brønsted Acid-Catalyzed Reaction Methodology Part A: Enantioselective Mannich Reaction Part B: Enantioselective Desymmetrization Of Meso-Aziridines, Emily Bretherick Rowland

USF Tampa Graduate Theses and Dissertations

The synthesis of enantiomerically pure compounds is of vital importance. Most biologically active natural products are chiral and require asymmetric synthesis, chiral resolution, or the use of naturally chiral starting materials for their preparation. Organocatalytic enantioselective reaction methodology is a continuously growing area in organic chemistry. The use of organocatalysts as a potentially environmentally friendly alternative to metal catalysts is appealing to the pharmaceutical industry.

In this dissertation an enantioselective Mannich reaction using an organocatalyst was investigated. The reaction was between a ß-keto ester and an imine electrophile catalyzed by vaulted biphenanthrol (VAPOL) phosphoric acid. The reaction resulted in products …

Enantioselective, Bronsted Acid-Catalyzed Additions Of Nitrogen And Carbon Nucleophiles To Imines, Gerald B. Rowland Jr.

USF Tampa Graduate Theses and Dissertations

The development of enantioselective reaction methodology has been at the forefront of research in both academic and industrial research laboratories due to the importance of chiral molecules in biological systems. An emerging area of research in the development of enantioselective reaction methodology has been the development of organocatalytic reactions. Organocatalysis, the use of small, chiral organic molecules as catalysts, has the advantage over traditional Lewis acid catalysis in that the reactions in general produce less toxic by-products. One recent breakthrough in the development of enantioselective methodology has been the development of chiral phosphoric acids as organocatalysts. Chiral phosphoric acids have …

Pandemic Vibrio Parahaemolyticus: Defining Strains Using Molecular Typing And A Growth Advantage At Lower Temperatures, Carisa Renee Davis

USF Tampa Graduate Theses and Dissertations

Vibrio parahaemolyticus is a leading cause of seafood-borne illness with a newly emerged pandemic strain. Previous studies compared the pandemic and non-pandemic strains to understand the evolution of the pandemic strain but no definitive explanation for its emergence has been discovered. This study investigated the molecular characteristics of the pandemic strain and growth characteristics at different temperatures. The hypothesis tested was that pandemic strains of V. parahaemolyticus have modifications to their proteome that give a selective advantage over the other V. parahaemolyticus strains at temperatures normally encountered in the environment. Molecular typing techniques; automated ribotyping, pandemic specific PCR and multilocus …

Psycho-Socio-Cultural Risk Factors For Breech Presentation, Caroline Peterson

USF Tampa Graduate Theses and Dissertations

The Breech Baby Study is a mixed methods study which combines qualitative and quantitative inquiry. This study explores psycho-social-cultural risk factors for breech presentation from an evolutionary perspective. The quantitative component of the study uses Florida birth certificate and Medicaid data sets from 1992-2003 to evaluate the influence of ethnicity and socio-economic status on breech presentation.

Ethnicity and socio-economic status account for less than two percent of the variance of risk factors for breech presentation. The qualitative study includes 114 mothers of breech and cephalic presentation babies who completed the State Trait Personality Inventory and a socio-demographic survey. Of these, …

Eutrophication Trend Of Lakes In The Tampa Bay Watershed And The Role Of Submerged Aquatic Vegetation In Buffering Lake Water Phosphorus Concentration, Max Jacobo Moreno Madriñan

USF Tampa Graduate Theses and Dissertations

Twentieth century human settlement within the Tampa Bay watershed was linked to a dramatic mid-century decline in bay water quality and loss of seagrass acreage. Decades of direct and indirect nutrient discharges to the bay from phosphorus mining, fertilizer manufacturing, and wastewater treatment, as examples, impaired the estuary. In the past twenty years, regional stakeholders have worked to improve the bay water quality by reducing point and non-point source nutrient loading to the bay.

Lakes within the Tampa Bay watershed may play an important role in attenuating the flow of nutrients into the bay. This study hypothesized that between 1990 …

The Role Of The Theory Of Planned Behavior In Therapists’ Involvement Of Parents In Youth Treatment, Sherecce Antoinette Fields

USF Tampa Graduate Theses and Dissertations

The theory of planned behavior has been studied in a wide variety of health related research. One area that has not evaluated the relevance of the TPB is that of therapists' attitudes for involving parents in treatment. The current study examined the feasibility of Ajzen's (1985) Theory of Planned Behavior for explaining whether or not therapists include parents in treatment. Participants in this study were therapists with at least one-year experience in treating youth under the age of 11. It was hypothesized that all of the variables of the TPB would be significant predictors of therapists' intention to include parents …

Sustainable Control Of Ascaris Lumbricoides (Worms) In A Rural, Disease Endemic And Developing Community: A Systems Approach, Monica Annmarie Gray

USF Tampa Graduate Theses and Dissertations

Parasitic infections, inadequate sanitation, and poor nutrition represent major etiologies that operate in synergy to cause some of the world's most disabling diseases. Citizens of developing nations, especially children living in rural areas, are the most affected. Current research and subsequent interventions have attempted to solve these issues using vertical interventions aimed at minimizing specific health outcomes. This approach does not consider the interaction among causes and the interrelationship between human beings and their environment. Challenges solved in this manner often fail to produce sustainable results or worse, create new problems.

This project proposed the systems approach framework to address …

Characterization Of The Western Antarctic Peninsula Ecosystem: Environmental Controls On The Zooplankton Community, Marina Marrari

USF Tampa Graduate Theses and Dissertations

The zooplankton community of Marguerite Bay, western Antarctic Peninsula, was investigated in relation to variability in chlorophyll concentrations and sea ice dynamics, using a combination of satellite remote sensing techniques and plankton net data. SeaWiFS chlorophyll data were validated with concurrent in situ data measured by HPLC and fluoromentric methods, and results indicate that SeaWiFS chlorophyll is an accurate measure of in situ values when HPLC data are used as ground truth.

Climatology data of SeaWiFS chlorophyll west of the Antarctic Peninsula showed that the Bellingshausen Sea and Marguerite Bay usually had higher and more persistent chlorophyll concentrations compared with …

Modulation Of Monocyte-Derived Dendritic Cell Maturation And Function By Cigarette Smoke Condensate In A Bronchial Epithelial Cell Co-Culture Model, Alison J. Montpetit

USF Tampa Graduate Theses and Dissertations

Lung airway epithelium is the first line of defense against inhaled particulates such as cigarette smoke. Subepithelial dendritic cells (DC) survey the airway epithelial lining and represent the link between innate and adaptive immune response. No study has investigated the effect of cigarette smoke on DC in the presence of epithelial cells (EC). The purpose of this 4x2 factorial design study was to co-culture normal human bronchial epithelial (NHBE) cells with monocyte-derived dendritic cells (MDDC) and examine the effect of cigarette smoke condensate (CSC) and poly I:C stimulation (TLR3 ligand that mimics viral infection) on MDDC homotypic clustering, phagocytosis ability, …

The Social Construction Of A Special Needs Program For Hurricanes, Robert E. Tabler Jr., M.A., C.H.E.S.

USF Tampa Graduate Theses and Dissertations

The overall purpose of this exploratory study was to comprehend how in the event of a hurricane Hillsborough County, Florida protected its elderly and disabled residents with special medical needs. This study used Social Constructionist Theory as a framework and Grounded Theory methodology in the collection of qualitative data.

To understand stakeholder knowledge and how they constructed the SpNP, three focus groups were conducted, with representatives from agencies on the Planning Committee. Through 30 in-depth, semi-structured interviews, clients of the SpNP, provided insight into their knowledge of the program and how society influenced evacuation decisions. Finally, 10 in-depth, semi-structured interviews …

Disgusted By Food: Explanatory Models Of Anorexia Among Young Taiwanese Adults, Donald E. Mclawhorn Jr.

USF Tampa Graduate Theses and Dissertations

Anorexia as a nosological category has developed in a western context and is now being applied to people around the world. In order for researchers to know they are asking the right questions about AN as knowledge expands, it is important to understand what meanings Anorexia carries and how those meanings manifest locally. The present study to aid in that understanding by employing a mixed methods (survey and in-depth interviewing) research approach in answering the following question: In what ways are Taiwanese students' explanatory models of anorexia nervosa (AN) congruent with or different from professional understandings derived from the western …

Quantifying Soil Organic Carbon (Soc) In Wetlands Impacted By Groundwater Withdrawals In West-Central Florida, Katherine Moore Powell

USF Tampa Graduate Theses and Dissertations

Saturated for most of the year, wetlands accumulate large amounts of biomass in thick organic soil horizons with slow rates of decomposition due to anaerobic conditions. Wetland soils thereby sequester large amounts of organic carbon in relative long-term storage. Municipal water demands in west-central Florida are largely met through extensive groundwater pumping. These withdrawals can impact ecosystems dependent on surface water levels that are ultimately linked to confined aquifers. Soils in a subset of cypress swamps that are monitored by the Southwest Florida Water Management District (SWFWMD) were sampled and analyzed to ascertain the health of the wetlands impacted by …

Location Awareness In Cognitive Radio Networks, Hasari Celebi

USF Tampa Graduate Theses and Dissertations

Cognitive radio is a recent novel approach for the realization of intelligent and sophisticated wireless systems. Although the research and development on cognitive radio is still in the stage of infancy, there are significant interests and efforts towards realization of cognitive radio. Cognitive radio systems are envisioned to support context awareness and related systems. The context can be spectrum, environment, location, waveform, power and other radio resources. Significant amount of the studies related to cognitive radio in the literature focuses on the spectrum awareness since it is one of the most crucial features of cognitive radio systems. However, the rest …

Behavior Contracting With Dependent Runaway Youth, Jessica Colon

USF Tampa Graduate Theses and Dissertations

The number of dependent youth reported as runaways to the Florida Department of Law Enforcement has become an increasing concern to the Department of Children and Families (Child Welfare League of America, 2005). Youth under state supervision, who are reported as runaways, most often leave from foster care settings, although some youth are also reported as runaways from the homes of relatives, non-relatives, and biological parents (CWLA, 2005). Community based care (CBC) agencies responsible for the supervision of dependent children in the State of Florida have struggled to develop an effective means of addressing the problem of running away and …

A 1400 Year Multi-Proxy Record Of Hydrologic Variability In The Gulf Of Mexico: Exploring Ocean-Continent Linkages During The Late Holocene, Jennifer A. Flannery

USF Tampa Graduate Theses and Dissertations

Late Holocene climate variability includes the Little Ice Age (LIA, 450-150 BP) and the Medieval Warm Period (MWP, 1100-700 BP) that are characterized by contrasting hydrologic and thermal regimes. The degree of interaction between the North American continent and the ocean during these two abrupt climate events is not well known. Marine sedimentary records from basins proximal to major rivers integrate climate signals across large spatial scales and can provide a coherent, high-resolution assessment of the oceanic and continental responses to changing climate and hydrologic conditions. The Pigmy Basin in the northern Gulf of Mexico is ideally situated to record …

Antifreeze Proteins In Pelagic Fishes From Marquerite Bay (Western Antarctica), Tammy L. Cullins

USF Tampa Graduate Theses and Dissertations

The Southern Ocean is home to two major types of fishes: endemics in the suborder Notothenioidii and representatives of oceanic fish families that are widely distributed in the midwater and benthic environments elsewhere (e.g. bathylagids, myctophids, liparids, and zoarcids) In most regions of the coastal Antarctic, e.g. the Ross Sea, there is a distinct separation in the pelagic communities at the shelf break between the oceanics (off-shelf) and the endemics (on-shelf). Coincidentally, in much of the coastal Antarctic, the shelf break also marks the boundary between a water column entirely composed of the very cold (-2°C ) Ice Shelf Water …