Digital Commons Network^™

Determination Of Decay Rates And Differential Survival Of Escherichia Coli And Enterococcus Spp. Strains Under Hydrodynamically Active Conditions Using Box-Pcr Typing, Phoebe West Koch

USF Tampa Graduate Theses and Dissertations

Indicator organisms (IOs) such as Escherichia coli and Enterococcus spp. are used to predict the presence of pathogens in waters. Determining the relationships between environmental factors, IOs, and pathogens is a key to assessing water quality and ensuring public health, yet certain strains of E. coli and Enterococcus spp. survive for long periods in natural waters. Molecular subtyping, using repetitive extragenic palindromic DNA sequences (BOX-PCR), has been used to discriminate among environmental E. coli and Enterococcus spp. isolates. The reproducibility of BOX-PCR patterns varies with DNA purification methods; therefore, it is important to develop a standardized, rapid, high throughput DNA …

The Builders Versus The Birds: Wetlands, People And Public Policy In The United States, Florida And Hillsborough County, Allyson R. Bennett

USF Tampa Graduate Theses and Dissertations

This thesis is an interdisciplinary analysis of humans' relationship to the natural environment, specifically how wetlands are reflected in our legislative decisions. Our perceptions of wetlands and our relationship to the environment are influenced by our locality, history, and inter-generational relationships. These perceptions shape decision-making within a community. Our relationship to the natural environment and the way we interact with it can be explained through psychological and geographical theories. Historical trends reveal our consistently negative perspectives of wetlands in the United States and a rapid decline in wetlands acreage. At the federal, state, and local level, Americans have attempted to …

Multi-Level Regulation Of Argininosuccinate Synthase: Significance For Endothelial Nitric Oxide Production, Karen Davidowitz Corbin

USF Tampa Graduate Theses and Dissertations

The citrulline-nitric oxide (NO) cycle, comprised of the enzymes argininosuccinate synthase (AS), argininosuccinate lyase (AL) and endothelial nitric oxide synthase (eNOS), is responsible for the regulated production of endothelial NO. Although most studies have focused on eNOS to uncover important regulatory mechanisms, we and others have determined that AS is an essential and regulated step in endothelial NO production. AS is rate limiting for endothelial NO production and is the primary source of arginine, the substrate for eNOS-mediated NO production, despite saturating intracellular levels of arginine and available arginine transport systems. AS is essential for endothelial cell viability and its …

Application Of Pd Catalyzed Alkylation: Synthesis Of Bicyclic Furans, Isoxazolines And New Cyclopentane Amino Acid Analogs, Pasha Moeenuddin Khan

USF Tampa Graduate Theses and Dissertations

Palladium is probably the most useful metal in organic syntheses. It has shown great utility in various reactions such as C-C, C-N, C-O bond formation under mild conditions. The presence of abundant amount of palladium-chemistry related literature in the form of books, reviews emphasizes the growing importance of these reagents. Nowadays organopalladium chemistry is being used in various fields such as new methodology development, natural product synthesis, synthesis of polymers. Regio- and stereoselectivity is another facet of Pd catalyzed methodologies which has been extensively utilized in the last decade to obtain enantiopure compounds. The main emphasis of this work is …

A Comparative Study Of Six Decades Of General Science Textbooks: Evaluating The Evolution Of Science Content, Anna Lewis

USF Tampa Graduate Theses and Dissertations

This study examined science textbooks over time to better understand the science content expectations that the U.S. educational system deems appropriate for 8th and 9th grade science students. The study attempted to answer the questions: 1) What specific science content has been presented via the textbook from 1952 to 2008? 2) Within which areas and in what way does the science content change? 3) Are new scientific findings reflected in 8th and 9th grade U.S. general science textbooks? Twenty-six themes were identified which reflect five areas in science: Chemistry, Physics, Earth Science, Biology, and Process of Science. Trends in science …

Hemingway’S Development Of The Female Characters Catherine From A Farewell To Arms To The Garden Of Eden, Amy K. Recla

USF Tampa Graduate Theses and Dissertations

This paper explores the possibility that Hemingway scholars are overlooking the development of Hemingway as a writer by concentrating too much on the autobiographical elements of his writing. I am not suggesting that scholars ignore the autobiographical aspects of the writing, but rather propose that scholars acknowledge and look for the development of Hemingway's craft of writing in his novels by comparing the early texts with his posthumous works. I have chosen to show this development by comparing A Farewell to Arms and The Garden of Eden, especially through his use of the females characters Catherine in both novels. …

Measuring Culture Change As An Evaluation Indicator: Applying Cultural Consensus Analysis To Cultural Models Of Lymphatic Filariasis In Haiti, Kelly M. Simpson

USF Tampa Graduate Theses and Dissertations

Introduction: This project explores the links between shared cultural beliefs in the illness domain, specific to lymphatic filariasis, and a support group program implemented in three Haitian towns. The purpose is to introduce an innovative approach to evaluation, the cultural model evaluation technique (CM Evaluation), as well as gain an understanding of the shifting cognitive belief structure around the cultural domain of lymphatic filariasis in the Haitian setting as associated with a support group intervention.

Method: The sample population was comprised of 241 women across three sites in Haiti: Archaie, Cabaret, and La Plaine. Data were collected from longitudinal surveys …

The Drama Of Collaborative Creativity: A Rhetorical Analysis Of Hollywood Film Making-Of Documentaries, Robert M. González Jr.

USF Tampa Graduate Theses and Dissertations

Current creativity research is dominated by attention to the individual, with increasingly less attention paid to creativity in its context, in groups, and in filmmaking as a collaboratively creative enterprise. This study answers the research call to explore filmmaking as an exemplar for collaborative creativity. Utilizing the stories told on DVD extras on special edition releases of feature films, this study analyzes how collaborative creativity is storied. In turn, these stories reveal specific communication forms, practices, and strategies that enrich theoretical conceptions of collaborative creativity. Following dramatistic concepts elaborated by Kenneth Burke, this rhetorical analysis finds three emergent patterns of …

From Behind Closed Doors To The Campaign Trail: Race And Immigration In British Party Politics, 1945-1965, Nicole M. Chiarodo

USF Tampa Graduate Theses and Dissertations

Colored immigration from the New Commonwealth became a highly discussed issue in British party politics immediately following the Second World War, but in an effort to sustain Great Britain's imperial status, to portray the nation as a new enlightened force, and in reaction to the race issues taking hold in the American South, political elites moved to keep their debates concealed from the public. In this thesis, I investigate the positions of Labour and Conservative governments and the circumstances surrounding the emergence of race and immigration into the public sphere. Moreover, I examine the effects of its arrival. While the …

Access To Health Care Services: A Case Study In Hillsborough County, Florida, Jaime Nodarse

USF Tampa Graduate Theses and Dissertations

The UpliftU® program is a long-term residential program for women and families who are homeless or at risk for homelessness. This program is one part of a larger, community-based non-profit organization serving low-income and homeless families in Hillsborough County, Florida for over 35 years. This program is not an emergency shelter program, but rather offers up to 18 months of participation in a self-sufficiency program to single women and families. The goal of the program is to prevent future homelessness for residents by helping them to reach their highest level of self-sufficiency. After volunteering at this organization for nine months, …

Electromagnetic Modes In Cylindrical Structures, Jakub Pritz

USF Tampa Graduate Theses and Dissertations

Nanostructures have received much attention from the physical and engineering communities in the past few years. The understanding of the behavior of nanostructures in various conditions is warranted since the applications of such materials in optics, electronics, and mechanics is ever expanding. This thesis investigates a specific type of structure, a concentric cylindrical. More specifically, the dispersion relation of radiating and non-radiating plasmon polaritons (quasi-particles resulting from interactions of photons and surface electrons) is studied under varying conditions. We intend to show the influence of changing the thickness of the layers, the number of layers, the curvature of each layer, …

The Role Of Abiotic And Biotic Factors In Suspension Feeding Mechanics Of Xenopus Tadpoles, William G. Ryerson

USF Tampa Graduate Theses and Dissertations

As a comparison to the suction feeding mechanics in aquatic environments, I investigated buccal pumping in an ontogenetic series of suspension feeding Xenopus laevis tadpoles (4-18 mm snout-vent length) by examining the morphology, kinematics, fluid flow, pressure generated in the buccal cavity, and effects of viscosity manipulation. Investigation of the dimensions of the feeding apparatus of Xenopus revealed that the feeding muscles exhibited strong negative allometry, indicating that larger tadpoles had relatively smaller muscles, while the mechanical advantage of those muscles did not change across the size range examined. Buccal volume and head width also exhibited negative allometry: smaller tadpoles …

Can Waters Around Durney Key, Pasco County, Florida, Support Coral Recruitment To Artificial Substrates?, Kelley L. Anderson

USF Tampa Graduate Theses and Dissertations

To determine whether an artificial reef installation is feasible, there must be a thorough characterization of the habitat. An understanding of both small-scale and large-scale environmental processes is needed to determine factors that potentially will influence the reef. Large-scale processes include coastal circulation, wave climate, and sediment dynamics that take place over spatial scales of tens to hundreds of kilometers in the region of the reef. Small-scale processes include the physical characteristics in the immediate vicinity of the reef - the local current, wave and tide characteristics, temperature, salinity, and suspended and bottom sediments at a proposed reef site.

The …

School As A Center For Community: Establishing Neighborhood Identity Through Public Space And Educational Facility, Fred Goykhman

USF Tampa Graduate Theses and Dissertations

“Safety is an opportunity for people to open their minds” -Jin Baek, 2008

For my thesis I will design an education facility. That education facility will strive to meet with today's security needs and will provide a safe-feeling place for growth. In identifying the problem, I found two main causes for the described conditions in today's schools. They are improper adaptation and uniform building type.

Improper adaptation has to do with surface applications, rather than integrating with the social fabric of the school's communal requirements. Unfortunate incidents have caused the solutions to heightened security around schools to be fortressing and …

Contradictions In A Distance Content-Based English As A Foreign Language Course: Activity Theoretical Perspective, Irshat Madyarov

USF Tampa Graduate Theses and Dissertations

This study explores six English as a foreign language students in an English content-based course of critical thinking delivered via distance at the Bahá'í Institute for Higher Education (BIHE) in Iran. Framed within cultural-historical activity theory, the study seeks to shed light on the complex nature of students' course-related activities with a particular focus on contradictions that underlie any human activity. The construct of contradictions provides a theoretical lens to understand the complex web of relationships among a number of elements in the course taking activity situated in a cultural-historical setting beset with political controversies, technological challenges, and demands of …

Cobalt(Ii)-Catalyzed Atom/Group Transfer Reactions: Stereoselective Carbene And Nitrene Transfer Reactions, Joshua V. Ruppel

USF Tampa Graduate Theses and Dissertations

Metalloporphyrins have been shown to catalyze many fundamental and practically important chemical transformations, some of which represent the first demonstrations of these catalytic processes. The most notable examples include an assortment of atom/group transfer reactions, such as oxene, nitrene, and carbene transfers. Atom/group transfer reactions allow for the direct conversion of abundant and inexpensive alkenes and alkanes into value-added functional molecules. Previous reports from our group have shown that cobalt-porphyrin based carbene and nitrene transfer reactions are some of the most selective and practical catalytic systems developed for cyclopropanation and aziridination. Backed by a family of D₂-symmetric chiral …

Effects Of Age And Hearing Loss On Perception Of Dynamic Speech Cues, Mei-Wa Tam Szeto

USF Tampa Graduate Theses and Dissertations

Older listeners, both with and without hearing loss, often complain of difficulty understanding conversational speech. One reason for such difficulty may be a decreased ability to process the rapid changes in intensity, frequency, or temporal information that serve to differentiate speech sounds. Two important cues for the identification of stop consonants are the duration of the interruption of airflow (i.e., closure duration) and rapid spectral changes following the release of closure.

Many researchers have shown that age and hearing loss affect a listener's cue weighting strategies and trading relationship between spectral and temporal cues. The study of trading relationships between …

The Biomechanics And Evolution Of Shark Teeth, Lisa Beth Whitenack

USF Tampa Graduate Theses and Dissertations

Measuring the effects of morphology on performance, and performance on fitness, is necessary to gain a full picture of selection, adaptation, ecology, and evolution. The performance of an organism's feeding apparatus, of which teeth are an integral part, has obvious implications for its fitness and survival. Extant shark teeth encompass a wide variety of shapes, and are often ascribed qualitative functions without any biomechanical testing, employing terminology such as gripping, piercing, crushing, cutting, or tearing. Additionally, teeth also comprise the vast majority of the fossil record of sharks. Therefore to understand the evolution of the shark feeding mechanism, we must …

Health And Behavioral Problems Associated With Symptoms Of Pediatric Sleep Disorders, Rachel B. French

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine prevalence rates of symptoms of several sleep disorders in young children, and the relationship between symptoms of pediatric sleep disorders and other childhood problems. Two-hundred-seventy-six children aged 2 to 5 years were studied through examination of a pre-existing database. Children rated as high risk for having a sleep disorder displayed significantly more aggressive behavior and attention problems, as compared to children whose sleep was rated in the normal range. However, no relationship was found between symptoms of sleep disorders and body mass index, asthma, or allergies. In addition, no relationship was found …

Investigation Of Visual Fields And Visually-Mediated Behavior In The Bonnethead Shark (Sphyrna Tiburo), Amy L. Osmon

USF Tampa Graduate Theses and Dissertations

The goal of this dissertation was to further examine the visual system and its importance to the bonnethead shark (Sphyrna tiburo). This species of hammerhead shark possesses the least amount of lateral cephalofoil expansion. Better understanding of their visual system and potential visually-mediated behaviors may increase understanding regarding adaptive benefits of their unique head shape. The dissertation revealed four factors regarding this species' visual system: 1) the extent of their optical visual fields span between 68-72 degrees laterally and cover their visual horizon, 2) they possess a fairly large (approximately 112 degree) blind spot directly in front of …

Prey-Size Selectivity In The Bivalve Chione In The Florida Pliocene-Pleistocene: A Reevaluation, Shubhabrata Paul

USF Tampa Graduate Theses and Dissertations

Previous study of drilling predation on the bivalve Chione during the late Neogene of Florida suggested that prey-size selectivity of predators was disrupted by species turnover and morphological change within the prey genus. More recent experimental work, however, showed that at least some of these changes can be attributed to the confounding effects of facies shifts between naticid-dominated, muricid-dominated, and mixed predator assemblages. As muricids have the most abundant and continuous fossil record and are most responsible for predation on the Chione bivalve in modern benthic ecosystems of Florida, we use new criteria to isolate the muricid component of the …

Microsphere-Aided Characterization Of Stimuli-Responsive Polymer Networks, Carlos A. Bello

USF Tampa Graduate Theses and Dissertations

The fabrication and characterization of surface-anchored hydrogel microstructures are described. The hydrogel structures are constructed from poly(N-isopropylacrylamide), or poly(NIPAAm), which is a well-known thermoresponsive polymer that swells and contracts with changes in temperature. When patterned on a surface, these structures can experience a variety of shape changes induced by nonuniform swelling. Depending on the aspect ratio, patterns can, for instance buckle upon swelling and form wave-like patterns. Such structural changes replicate oscillatory motion of the smooth muscle cells and can be used to transport objects in microfluidics. The work, herein, investigates methods of pattern production and introduces a new technique …

Characterizing The In-Vitro Morphology And Growth Kinetics Of Intermediate Amyloid Aggregates, Shannon E. Hill

USF Tampa Graduate Theses and Dissertations

The mechanisms linking deposits of insoluble fibrils of amyloid proteins to the debilitating neuronal cell death characteristic of neurodegenerative diseases remain enigmatic. Recent findings suggest that transiently formed intermediate aggregates, and not the prominent neuronal plaques, represent the principal toxic agent. Evaluating the neurotoxicity of intermediate aggregates, however, requires unambiguous characterization of all aggregate structures present, their relative distributions, and how they evolve in time. Hen-egg white lysozyme represents an attractive model for studying intermediate aggregate formation since it is an extensively characterized globular protein, and its human variants can lead to systemic amyloidosis.

Combining in-situ dynamic light scattering (DLS) …

Aging With Identity: Integrating Culture Into Senior Housing, Christine Sanchez

USF Tampa Graduate Theses and Dissertations

There is an underlying hope that those whom are elderly can exist in a place of happiness, comfort, security and familiarity; and can do so by aging in a place which complies with an understanding of their needs as older adults. However, along with aging comes a negative association of dependence and deterioration. Therefore, it is intuitive to have a connection between both the aging process and the design process. Creating a product which brings a positive association with senior housing and aging in place is the underlying goal within the project and overall design.

With the increasing population of …

An Ethologically Relevant Animal Model Of Post-Traumatic Stress Disorder: Physiological, Pharmacological And Behavioral Sequelae In Rats Exposed To Predator Stress And Social Instability, Phillip R. Zoladz

USF Tampa Graduate Theses and Dissertations

Post-traumatic stress disorder (PTSD) is a debilitating mental illness that results from exposure to intense, life-threatening trauma. Some of the symptoms of PTSD include intrusive flashback memories, persistent anxiety, hyperarousal and cognitive impairments. The finding of reduced basal glucocorticoid levels, as well as a greater suppression of glucocorticoid levels following dexamethasone administration, has also been commonly observed in people with PTSD. Our laboratory has developed an animal model of PTSD which utilizes chronic psychosocial stress, composed of unavoidable predator exposure and daily social instability, to produce changes in rat physiology and behavior that are comparable to the symptoms observed in …

Sedimentation Of Organic - Inorganic Composites By Optical Turbidity, Reshma K. Harrinauth

USF Tampa Graduate Theses and Dissertations

Sedimentation is one of many characterization tools used to test materials in nanotechnology. Characterization of settling behavior is complex as there are many variables which can affect sedimentation. In our research, we focused on sedimentation in colloidal systems with the aid of an optical turbidometer. Nanoparticles of CeO₂ (Ceria Oxide) and TiO₂ (Titanium Dioxide) are embedded onto a polymeric matrix of a thermally responsive microgel of poly(N-isopropylacrylamide) (PNIPAM) and interpenetrating chains of poly(acrylic acid) to create novel composites. The composites are loaded with the inorganic oxide nanoparticles at different weight percent from a low value of 10 weight …

Reburb: Redefining The Suburban Family Unit Under A New Construction Ecology, Matthew A. Lobeck

USF Tampa Graduate Theses and Dissertations

“Our structures might be machines for living in, but there was no longer much about them that was alive.”

-William McDonough, Cradle to Cradle

Home ownership is a significant driver within American culture. In Florida single-family homes represent one of the largest components of our built surroundings, significantly impacting the environment through material use and energy consumption. Currently, homes typically are built with little regard to the environmental context. By designing for the immediate goal of separation from the elements, they do not provide for convenient spatial expansion or adaptability, using material assemblies that do not lend themselves to be …

The Virtual Landscape Of Geological Information Topics, Methods, And Rhetoric In Modern Geology, Sarah Elizabeth Fratesi

USF Tampa Graduate Theses and Dissertations

Geology is undergoing changes that could influence its knowledge claims, reportedly becoming more laboratory-based, technology-driven, quantitative, and physics- and chemistry-rich over time. This dissertation uses techniques from information science and linguistics to examine the geologic literature of 1945-2005. It consists of two studies: an examination of the geological literature as an expanding network of related subdisciplines, and an investigation of the linguistic and graphical argumentation strategies within geological journal articles.

The first investigation is a large-scale study of topics within articles from 67 geologic journals. Clustering of subdiscipline journals based on titles and keywords reveals six major areas of geology: …

Impact Of Seizure-Related Variables And Psychopathology On Health-Related Quality Of Life In Pediatric Epilepsy, Aja M. Meyer

USF Tampa Graduate Theses and Dissertations

Psychopathology typically is a lasting condition that is persistent from childhood to adulthood. Therefore, it is imperative that children with health conditions and comorbid psychiatric disorders are treated for both conditions as they are likely to have a significant negative impact on children's overall health-related quality of life (HRQL). More specifically, it is important to identify the variables that affect HRQL in children with epilepsy. Research has shown that biomedical variables such as seizure severity and frequency have only moderate relationships with HRQL; therefore, additional factors, such as depression and anxiety, must be identified so that they also may be …

Talking With Exotic Pet Owners: Exploratory Audience Research On Wildlife Television And Human-Animal Interactions, Susannah L. Smith

USF Tampa Graduate Theses and Dissertations

This qualitative grounded study explores the potential relationship between wildlife TV viewing and human-animal interactions for exotic pet owners. The method involved 13 in-depth interviews and a qualifying open-ended questionnaire with 37 individuals. The interviews gathered viewers' interpretations of two different human-wildlife interactions on TV and served as a launching point for discussion. Findings supported the literature in that wildlife TV was an important source of information, emotion, and contradictory messages. Themes also emerged regarding participants' characterizations of their relationships with their pets. Drawing from social cognitive theory, this thesis suggests the following potential motivators for participants to model animal …