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The Female Colonizer And Othered Woman In Isak Dinesen's Out Of Africa, Jean Rhys's Wide Sargasso Sea, Tayeb Salih's Season Of Migration To The North, And Paule Marshall's The Chosen Place, The Timeless People, Lindsay L. Sloan

USF Tampa Graduate Theses and Dissertations

The central issue of this thesis is the complicated relationship between the colonized individual and the constitutive as well as emblematic female colonizer in Isak Dinesen's Out of Africa, Jean Rhys's Wide Sargasso Sea, Tayeb Salih's Season of Migration to the North, and Paule Marshall's The Chosen Place, The Timeless People. Each of these novels displays colonization by a female (or females) and relates back to historical colonialism, but each characterizes the relationship between the oppressors and oppressed differently. Dinesen's and Rhys's works stem from historical colonization in which European colonizers conquered and ruled other territories; …

Splicing Systems For Studying Signaling To The Spliceosome, Hercules S. Apostolatos

USF Tampa Graduate Theses and Dissertations

Alternative splicing is a major contributing factor to protein diversity. The human genome project validated that there are about 25,000 genes, but over 100,000 proteins. Genes contain numerous exons that are specifically regulated; thus, elucidating alternative splicing mechanisms of pre-mRNAs is an immense undertaking. Two basic tools are used to study this mammoth task. For in vitro studies, usually in vitro transcription followed by splicing assays is used. For in vivo studies, the main technique is the construction of minigenes. These techniques enable one to study the mechanisms/conditions that explain an exon's inclusion or exclusion in the mature mRNA.

In …

Unamuno's Concept Of The Tragic, Ernesto O. Hernandez

USF Tampa Graduate Theses and Dissertations

This thesis focuses in presenting Miguel de Unamuno’s concept of the tragic. Historically this concept has suffered various changes of meaning and application. If successful the project shall provide the distinct connotation, features, and characteristics that Unamuno attributes to the tragic. His special treatment of the tragic harnesses a way for the will to become aware of its existential condition. This awakening of consciousness evokes an arousal of dichotomies that the will must confront. Faith against reason, religion against science, heart against intellect, are amongst these conflicting predicaments. The will’s constant struggle between these opposing forces constitutes for Unamuno the …

Articulating Social Change In Puerto Rico: Environmental Education As A Model For Youth Socio-Political Development And Community-Led School Reform, Federico Cintrón-Moscoso

USF Tampa Graduate Theses and Dissertations

Recent attempts at developing an environmental education agenda in public schools emphasize the need to foster greater public awareness about environmental rights, issues, and solutions, while producing citizens with the knowledge and skills needed to address the ecological challenges of contemporary society. However, some scholars have argued that the attempt to integrate environmental principles into the school curricula has created a conflict between the politically-oriented goals of environmental education and the more passive practices of uncritical assimilation and reproduction found in many schools today (Stevenson 2007). Moreover, although there is a need for public schools to take on the challenge …

Life In The Florida Everglades: Bioarchaeology Of The Miami One Site, Cristina Echazabal

USF Tampa Graduate Theses and Dissertations

The bioarchaeology of prehistoric south Florida has been an area of archaeological interest for the last century because of the interplay between ancient populations and the unique environment of the Everglades. The purpose of this study is to analyze the pathology, demography and mortuary practice of the ancient Southeast Florida aboriginal population at Miami One to assess the similarity of Miami One to other south Florida populations during the prehistoric period. The Miami One site (8DA11) is one of many related sites located along the shore of the Miami River. It was continuously occupied from the Late Archaic (ca.1000 B.C.) …

Effects Of Ozone On Blood Components, Daniela Sloan

USF Tampa Graduate Theses and Dissertations

Previous studies on the medical use of ozone therapies show a very diverse array of results, from ozone reducing the amount of HIV virus in the blood, to no effect, to causing the death of several patients due to pulmonary embolism and infections. However, ozone therapies are widely used in Europe and considered medically safe. In the U.S., doctors in 28 states use ozone therapies.

The objectives of this study were to investigate the effects of medical grade ozone at varying concentrations used in ozone therapies. These were achieved by evaluating the C-reactive protein, erythrocyte sedimentation rate, total reduced and …

Descartes' Bête Machine, The Leibnizian Correction And Religious Influence, John Voelpel

USF Tampa Graduate Theses and Dissertations

René Descartes’ 1637 “bête machine” characterization of nonhuman animals has assisted in the strengthening of the Genesis 1:26 and 1: 28 disparate categorization of nonhuman animals and human animals. That characterization appeared in Descartes’ first important published writing, the Discourse on the Method, and can be summarized as including the ideas that nonhuman animals are like machines; do not have thoughts, reason or souls like human animals; and thus, cannot be categorized with humans; and, as a result, do not experience pain or certain other feelings. This characterization has impeded the primary objective of environmental ethics - the extension of …

Immune Redistribution To Skin In Wild And Domesticated Songbirds, Joshua Ryan Kuhlman

USF Tampa Graduate Theses and Dissertations

Implantation of dental sponges under the skin of lab rodents has been used to evaluate whether acute stress enhances leukocyte infiltration to a surgical site. First, I replicated this technique in house sparrows to test whether transient stressors cause similar immunoredistribution (i.e., movement of immune cells out of circulation and to the periphery) in a wild animal. As placement into captivity alone may serve as a stressor to wild animals, I compared sponge infiltration over different periods of captivity. Second, I compared how domestication affects immunoredistribution by comparing results of wild sparrows to domesticated zebra finches. Zebra finches were chosen …

A Philosophical Inquiry On The Valuation And Selection Of Musical Materials For Culturally Diverse Learners In Global Environments, Jonathan Bassett

USF Tampa Graduate Theses and Dissertations

The selection of musical materials for use in the classroom is problematic. Since music educators have limited time with students, the inclusion of musical materials necessitates the perceived exclusion of other musical materials. This perception is due in part to the increasing diversity of students represented in the classroom, the cultural labeling of musical materials, the influence of multiculturalism on music education, and the influence of the Western aesthetic in music education. Since diverse groups can and do propose socially perceived valuations of these materials as critical for the inclusion of specific musical materials for use in the classroom, this …

Use Of Model Compounds To Study Potential Removal Of Pharmaceuticals Using Octolig®, Wen-Shan Chang

USF Tampa Graduate Theses and Dissertations

The existence of pharmaceuticals in the environment has some adverse effects, and may pose threat to the organisms in the environment. The possibility of removing certain pharmaceuticals from wastewater was tested using Octolig®, a commercially available material with polyethyldiamine moieties covalently attached to high-surface area silica gel. Selected drug models were subjected to column chromatography in efforts to effect removal by means of ion encapsulation, the effectiveness of which would depend upon having appropriate anionic functional groups. The experimental results suggested that the model compounds, Rose Bengal, Eosin Y, Erythrosine , ZPS, and Lissamine Green B were successfully encapsulated by …

Biosynthesis Of Fatty Acid Amides, Emma K. Farrell

USF Tampa Graduate Theses and Dissertations

Primary fatty acid amides (PFAMs) and N-acylglycines (NAGs) are important signaling molecules in the mammalian nervous system, binding to many drug receptors and demonstrating control over sleep, locomotor activity, angiogenesis, vasodilatation, gap junction communication, and many other processes. Oleamide is the best-studied of the PFAMs, while the in vivo activity of the others is largely unstudied. Even less is known about the NAGs, as their discovery as novel compounds is much more recent due to low endogenous levels. Herein is described extraction and quantification techniques for PFAMs and NAGs in cultured cells and media using solvent extraction combined with …

Anuran And Tree Community Structure Of Cypress Domes In Tampa, Florida, Relative To Time Since Incorporation Within The Urban Landscape, Christopher Haggerty

USF Tampa Graduate Theses and Dissertations

Wetlands in densely urbanized landscapes display altered physical, chemical, and biological features; however, long term trends in these are poorly known. The purpose of this study was to determine if biological, physical, and chemical changes in isolated cypress domes are related to time since incorporation within an urban landscape. Sixteen cypress domes in Tampa, Florida were placed into four decadal age groups based on construction date of residential homes within 200 m of wetland edge, with multiples in each age group: 1970s, 1980s, 1990s, 2000s, and rural sites as regional controls. Each site was visited monthly from July 2008 until …

Stochastic Modeling And Statistical Analysis, Ling Wu

USF Tampa Graduate Theses and Dissertations

The objective of the present study is to investigate option pricing and forecasting problems in finance. This is achieved by developing stochastic models in the framework of classical modeling approach.

In this study, by utilizing the stock price data, we examine the correctness of the existing Geometric Brownian Motion (GBM) model under standard statistical tests. By recognizing the problems, we attempted to demonstrate the development of modified linear models under different data partitioning processes with or without jumps. Empirical comparisons between the constructed and GBM models are outlined.

By analyzing the residual errors, we observed the nonlinearity in the data …

Toward An Applied Anthropology Of Gis: Spatial Analysis Of Adolescent Childbearing In Hillsborough And Pinellas Counties, Florida, Kathleen I. Maes

USF Tampa Graduate Theses and Dissertations

This work investigates births to white, African American and Hispanic adolescents in Hillsborough and Pinellas Counties, Florida, from 1992 to 1997 in two age groups - 13 to 17 year-olds and 18 to 19 year-olds - using spatial statistical techniques along with key informant interviews to provide insights into the utility of the research findings. The research developed a method for estimating the adolescent population in inter-census years, which was used to determine denominators for calculating teen birth rates. It also developed a composite deprivation index using socioeconomic indicators at the census block group level. The index provided context for …

The Effects Of Learning-Styles Information On The Achievement Of Community College Developmental Math Students, Kevin A. Hoeffner

USF Tampa Graduate Theses and Dissertations

Four out of five Americans will require some postsecondary education. Therefore, a majority of population will return to a community college for retraining and personal growth (McCabe, 2003). Since the turn of this century, many studies have been conducted to examine the success and challenges of the relatively new community college system. One of the most significant challenges is the large percentage of the U.S. population requiring remedial coursework. Fifty-five percent of students entering Florida's postsecondary system require remediation. Of this large remedial population, only 51% will complete their preparatory classes. Students who do complete classes take an average of …

Influence Of Crosslink Density On Swelling And Conformation Of Surface-Constrained Poly(N-Isopropylacrylamide) Hydrogels, Ryan S. Cates

USF Tampa Graduate Theses and Dissertations

A stimuli-responsive microgel is a three-dimensional polymer network that is able to absorb and expel a solvent (commonly water). These materials are unique in the fact that their sponge-like behavior can be actuated by environmental cues, like temperature, ion concentration, pH, and light. Because of the dynamic properties of these materials they have found applications in drug-delivery systems, micro-assays, selective filtration, artificial muscle, and non-fouling surfaces. The most well-known stimuli-responsive polymer is Poly(N-isopropylacrylamide) or PNIPAAm and it experiences a switchable swelling or deswelling over a critical temperature ( T_c=~32°C). Below the critical temperature, the gel begins mixing with …

Lived Experience: Near-Fatal Adolescent Suicide Attempt, Phyllis Ann Dougherty

USF Tampa Graduate Theses and Dissertations

Adolescent suicide has become a national health crisis. Suicide now ranks as a leading cause of adolescent death in the U.S. In response to this, the National Strategy for Suicide Prevention (2001b) recommended the promotion and support of research into suicide and prevention, particularly high-risk groups such as adolescents. However, due to concerns for safety and liability, there have been few studies of highly suicidal individuals, specifically adolescents. Leading suicidologists have agreed that studying the qualities of the near-fatal suicide attempt can most resemble the completed suicide.

This case study explored the phenomenon of the near-fatal suicide attempt through the …

Low Documented Risk Cesarean Sections And Late-Preterm Births: The Florida Experience, Heather Breeze Clayton

USF Tampa Graduate Theses and Dissertations

There are increasing concerns about the excessive use of cesarean delivery in the United States, as cesarean deliveries have been associated with adverse maternal and infant health outcomes. Currently, the cesarean section (C/S) rate for Florida is the second highest in the nation. Furthermore, preliminary reports from the Florida Department of Health (FDOH) have implicated the increasing rate of cesarean delivery to an increase in the rate of late preterm births (PTB) in Florida (births at 34 to 36 weeks gestational age). Information on the impact of late PTB associated with cesarean delivery on the rate of maternal and infant …

Active Surface Topographies In Constrained Hydrogel Films For Biomedical Applications, Ophir Ortiz

USF Tampa Graduate Theses and Dissertations

Lung cancer has the highest mortality rate relative to all types of cancers, and unfortunately there still exist numerous challenges towards decreasing this rate. One of these challenges is gaining a clear understanding of why metastatic lung cancer cells attach and detach to colonize other areas of the body. Reports suggest that the attachment of cells to secondary tumor sites does not occur randomly. It is theorized that both the physical and chemical properties of the tissue are able to create a suitable environment for their adhesion. Therefore, the motivation for the work presented herein is to use dynamic thermoresponsive …

Total Internal Reflection Holographic Microscopy (Tirhm) For Quantitative Phase Characterization Of Cell-Substrate Adhesion, William Mason Ash Iii

USF Tampa Graduate Theses and Dissertations

Total Internal Reflection Holographic Microscopy (TIRHM) combines near-field microscopy with digital holography to produce a new form of near-field phase microscopy. Using a prism in TIR as a near-field imager, the presence of microscopic organisms, cell-substrate interfaces, and adhesions, causes relative refractive index (RRI) and frustrated TIR (f-TIR) to modulate the object beam's evanescent wave phase front. Quantitative phase images of test specimens such as Amoeba proteus, Dictyostelium Discoideum and cells such as SKOV-3 ovarian cancer and 3T3 fibroblasts are produced without the need to introduce stains or fluorophores. The angular spectrum method of digital holography to compensate for …

Bird Communities Of Isolated Cypress Wetlands Along An Urban Gradient In Hillsborough County, Florida, Nathaniel L. Goddard

USF Tampa Graduate Theses and Dissertations

Migratory bird communities are sensitive to landscape alteration. Urban development significantly impacts bird communities on breeding grounds, as well as en-route during migration. One current theory is that Neotropical migratory birds are not limited by breeding or wintering habitat constraints but by food and habitat availability along major migration routes. The eastern flyway is the route taken by neotropical land-birds through eastern North America that follows coastal areas denoted by intense urban development. Coastal areas funnel birds to major departure points along the northern coast of the Gulf of Mexico and the western coast of Florida.

Birds were monitored for …

Characterization Of Cannabinoid Receptor 2 Transcript Expression In B Cells, Tracy Sherwood

USF Tampa Graduate Theses and Dissertations

Cannabinoids and cannabinoid receptors have been shown to play important roles in immune regulation particularly as modulators of anti-inflammatory cytokines and antibody production. The predominant cannabinoid receptor involved in this immune regulation is cannabinoid receptor 2 (CB₂), which is robustly expressed in B cells. Utilizing a combination of bioinformatics, 5' RACE, real time RT-qPCR, and reporter assays, we showed that human B cells from peripheral blood mononuclear cells (PBMC) expressed one CB2 transcript while mouse B cells from spleen express three CB₂ transcripts. Alignment of the sequenced B cell RACE products to either the mouse or human …

Statistical Learning And Behrens-Fisher Distribution Methods For Heteroscedastic Data In Microarray Analysis, Nabin K. Manandhr-Shrestha

USF Tampa Graduate Theses and Dissertations

The aim of the present study is to identify the di®erentially expressed genes be- tween two di®erent conditions and apply it in predicting the class of new samples using the microarray data. Microarray data analysis poses many challenges to the statis- ticians because of its high dimensionality and small sample size, dubbed as "small n large p problem". Microarray data has been extensively studied by many statisticians and geneticists. Generally, it is said to follow a normal distribution with equal vari- ances in two conditions, but it is not true in general. Since the number of replications is very small, …

School Psychologists Involvement And Perceived Preparedness In The Provision Of Suicide-Related Services: A Comparison Of Practitioners Serving Different School Levels, Jennifer M. Cunningham

USF Tampa Graduate Theses and Dissertations

While the manifestation of suicidal thoughts and/or behavior is more common among adolescents, children are capable of, and do experience, suicidal ideation as well as demonstrate suicidal behaviors. Suicide is the sixth leading cause of death among children aged 5-14 years (Center for Disease Control [CDC], 2008). However, children may not always be referred or brought to the attention of the school psychologist, as their threats may be considered immature and unfounded. The purpose of this study is to provide data that clarifies the need for the provision of suicide-related services for children in elementary school. An archival dataset of …

Exploring Colorectal Cancer Diagnosis Disclosure To First-Degree Relatives: An African American Family Case Series, Kamilah B. Thomas

USF Tampa Graduate Theses and Dissertations

Colorectal cancer (CRC) is the second leading cancer killer in the United States and the third most common cancer in African American men and women. Though the overall death rates have declined, this reduction in mortality is smaller for African Americans than for Whites. Factors that are protective against colorectal cancer include occupational or recreational physical activity, a diet high in fruits and vegetables, and colorectal cancer screening with removal of polyps (polypectomy) before they progress to cancer. Compliance with CRC screening recommendations requires people to know if a first-degree relative (parent, sibling, and child) or second-degree relative (aunt, uncle, …

Depositional Dynamics In Seagrass Systems Of Tampa Bay, Fl: Influence Of Hydrodynamic Regime And Vegetation Density On Ecosystem Function, Alison Cheryl Meyers

USF Tampa Graduate Theses and Dissertations

Many coastal ecosystems around the world are dominated by submerged aquatic vegetation (SAV) habitats. These SAV habitats are known to provide many highly valuable ecosystem services such as habitat for commercial important species and increased water clarity. Water flow is an environmental variable which can have measurable effects on the ecosystem services provided by SAV, but is often not considered in studies assessing these services. This dissertation sought to investigate the links between SAV, primarily seagrasses, and hydrodynamics, paying special attention to the effects on sediments and fauna. Three main areas are discussed: (1) the effects of SAV on flow, …

(Im•Print) A Material Investigation To Encourage A Haptic Dialog, Julie Marie Vo

USF Tampa Graduate Theses and Dissertations

The word impression encompasses a dual meaning which desires to be more fully explored in the built realm. An (im-print) has a powerful impact because the message becomes an indelible mark embedded within the material. Physically, an impression is made by the pressure of one object on or into another, leaving behind a trace of this interaction on the surface. This process has the potential to create a vivid memory within the participant who comes into contact with it.

The idea of imprint can become a part of the process of design both physically and conceptually. As polished concrete can …

Surface-Bonded Sol-Gel Sorbents For On-Line Hyphenation Of Capillary Microextraction With High-Performance Liquid Chromatography, Scott S. Segro

USF Tampa Graduate Theses and Dissertations

High-performance liquid chromatography (HPLC) is the most widely used analysis technique. However, its sensitivity is limited. Sample preconcentration methods, such as fiber-based solid-phase microextraction (SPME) and in-tube SPME (capillary microextraction) offer improved detection limits. It is, however, difficult to couple fiber SPME on-line with HPLC due to the need for complicated desorption devices. Such coupling is further complicated due to the limited solvent stability of the extracting phase both in the fiber and in-tube formats of SPME. In this research, surface-bonded sol-gel sorbents were developed to provide the solvent stability required for effective on-line hyphenation of capillary microextraction (CME) with …

Application Of Luminescence Sensors In Oxygen Diffusion Measurement And Study Of Luminescence Enhancement/Quenching By Metallic Nanoparticles, Sanchari Chowdhury

USF Tampa Graduate Theses and Dissertations

The first part of this dissertation deals with the application of a luminescence quenching method to measure diffusion and permeation coefficients of oxygen in polymers. Most luminescence oxygen sensors do not follow linearity of the Stern-Volmer (SV) equation due to heterogeneity of luminophore in the polymer matrix, thus the complexity of data analysis is increased. To circumvent this limitation, inverted fluorescence microscopy is utilized in this work to investigate the SV response of the sensors at the micron-scale. In these diffusion experiments, oxygen concentration is measured by luminescence changes in regions with high SV constants and good linearity. Thus, we …

Women And Architecture: Re-Making Shelter Through Woven Tectonics, Kirsten Lee Dahlquist

USF Tampa Graduate Theses and Dissertations

Weaving and architecture, conceived simultaneously with cave paintings, are two ancient forms of craft used to enclose space and provide shelter harmoniously with nature. In its basic composition, a useable textile is the interlacing of two members, warp and weft, at right angles to create structure and surface respectively. Textile artist Anni Albers of the Bauhaus attributes the organization of weaving to the skills of an ancient goddess. Her understanding of prehistoric cultures further links women closer to the overall creation of structure, though perceived as a masculine endeavor. Consequently, early advancements in architecture, the structural organization of shelter, are …