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Mediation And Moderation Analysis Of Nutrition, Inflammatory Biomarkers, And Cognition In Older Adults, Elizabeth Handing

USF Tampa Graduate Theses and Dissertations

Nutrition can be viewed as a modifiable factor related to maintaining and preserving health in older adults. Previous studies have found that nutritional factors can influence cognitive abilities, however few studies have examined macronutrients and micronutrients as they relate to cognitive functioning. Research has yet to examine the mechanisms related to nutrition, cognition and aging in an older adult population from a holistic and interactive perspective.

This dissertation examined three research questions to better understand the relationship between age, nutrition, cognition, and inflammatory biomarkers. First, is nutrition related to cognition beyond demographic factors? Do individual nutrients serve as mediators? Second, …

Exploring The Underlying Mechanisms Of Comorbid Adhd And Eating Disorders, Jennifer Rebecca Bleck

USF Tampa Graduate Theses and Dissertations

Evidence suggests comorbidity of ADHD and eating disorders (EDs) among females. Capitalizing on the comorbidity of ADHD and EDs and subsequent obesity could lead to improved prevention and treatment of all three conditions. However, additional information regarding the comorbidity is necessary to develop such interventions, as little is known about how or why this co-occurrence exists. A comprehensive model of the underlying mechanisms associated with comorbid ADHD and EDs is needed to improve understanding of the development of the comorbidity. Moreover, while there are gender differences within each disorder, literature is limited regarding to the comorbidity among males, leading to …

The Relation Between Adiposity And Anxiety In Youth: Analysis Of Peer Victimization, Teasing, Sociocultural Influences, And Internalization Of Appearance Ideals As Explanatory Variables, Natasha L. Burke

USF Tampa Graduate Theses and Dissertations

The purpose of the current study was to examine putative mediators and moderators in the association between adiposity and anxiety in a sample of overweight and obese youth. In addition, anxiety was examined as a potential moderator between adiposity and health-related quality of life (HRQOL). Participants were youth (N = 137) between 8 and 17 years old (M = 13.09, SD = 2.61) and their legal caregivers recruited from four medical clinics affiliated with the University of South Florida. Youth were primarily overweight (28.5%) or obese (64.2%) and ethnically diverse. Data were analyzed by path analysis. Weight-related teasing significantly mediated …

Latino Immigrant Workers’ Search For Justice After Occupational Injury, Carla Gabriela Castillo

USF Tampa Graduate Theses and Dissertations

Latino immigrants encounter an entanglement of rights and policies after occupational injury or illness. In collaboration with an immigrant worker center, ethnographic research and a survey are used to analyze injured workers’ experiences. The center uses survey results to identify common threads and systematic problems, and to explore potential direct action. Through interviews with workers and medical and legal professionals, I investigate the barriers Latino immigrants face following occupational injury or illness, how their lived experiences relate to the greater medicolegal frameworks that demarcate most formal processes of compensation and treatment, and the experiences of professionals who mediate these structures. …

High-Throughput Screening Of Age-Related Changes In Caenorhabditis Elegans, Neil Copes

USF Tampa Graduate Theses and Dissertations

This project was developed to identify novel methods for high-throughput culturing and screening of C. elegans to investigate age-related metabolic changes and to survey the proteomic and metabolomic factors associated with age-related changes. To accomplish these goals we developed a novel way to grow C. elegans in liquid culture in 96-well microplates for several weeks without suffering significant fluid loss due to evaporation and without needing to shake or unseal the plates for aeration. We also developed methods for assaying the total volume of live C. elegans in microplate cultures using a fluorescence microplate reader and for performing RNAi experiments …

Strategies For Preventing Age And Neurodegenerative Disease-Associated Mitochondrial Dysfunction, Vedad Delic

USF Tampa Graduate Theses and Dissertations

Mitochondrial dysfunction plays a pivotal role in the development of aging phenotypes and aging-associated neurodegenerative disorders, such as Alzheimer’s disease (AD), Parkinson’s disease (PD) and Amyotrophic lateral sclerosis (ALS). Strategies that restore mitochondrial dysfunction may rescue the deficits of central metabolism in these disorders and improve cell survival. For example, we found that modulating the mTOR signaling pathway in a tissue culture model of aging-induced mitochondrial DNA mutation enhanced mitochondrial function as evidenced by increased oxygen consumption. Our previous melatonin studies also led us to hypothesize that caloric restriction and the hormone melatonin would reverse brain mitochondrial dysfunction in animal …

Maternal Stress, Breastmilk Igf-1, And Offspring Growth Among Breastfeeding Mothers-Infant Pairs In The Tampa Bay Area, Lauren Michelle Gottfredson

USF Tampa Graduate Theses and Dissertations

Background: Maternal stress during utero has been shown to have negative health consequences on the offspring, including low birth weight and increased risk of adult disease. Variation in breastmilk may act as an environmental cue of maternal stress and continue to program the infant during early life. This research aimed to explore the role of breastmilk on developmental programming of the infant. Specifically, to examine how breastmilk may act as a medium for the exposure of stress between the mother and the offspring, and see if variation in insulin like growth factor-1 (IGF-1) a potential mechanism for the relationship.

Methods: …

Theoretically Guided Examination Of Caregiver Strain And Its Relationship With School-Based Mental Health Services Utilization And Parent Engagement In Services, Amy Lynn Green

USF Tampa Graduate Theses and Dissertations

Broadly, the purpose of this study was to address the gaps in the knowledge base of caregiver strain through an examination of this and other theoretically related constructs in a sample of parents of high-risk youth. In the last two decades, a growing body of research has pointed to the significance of strain that can result from this caregiving experience, particularly as it relates to patterns of mental health services utilization. Despite the fact that the majority of children who receive mental health receive them from the school, few studies have examined caregiver strain in the context of school-based mental …

Neuroinflammatory Alterations Via Cd-36 In Traumatic Brain Injury, Diana G. Hernandez-Ontiveros

USF Tampa Graduate Theses and Dissertations

Traumatic brain injury (TBI) has become an increasingly unmet clinical need due to intense military conflicts worldwide. Directly impacted brain cells suffer massive death, with neighboring cells succumbing to progressive neurodegeneration accompanied by inflammatory and other secondary cell death events. Subsequent neurodegenerative events may extend to normal areas beyond the core of injury, thereby exacerbating the central nervous system’s inflammatory response to TBI. Recently CD-36 (cluster of differentiation 36/fatty acid translocase (FAT), a class B scavenger receptor of modified low-density lipoproteins (mLDLs) in macrophages, has been implicated in lipid metabolism, atherosclerosis, oxidative stress, and tissue injury in cerebral ischemia, and …

Documenting Perceived Effectiveness Of Community-Based Health Promotion Coalitions: A Grounded Theory Approach, Alyssa Brooke Mayer

USF Tampa Graduate Theses and Dissertations

Introduction: Community coalitions with public health-related missions are formal, semi-permanent, action-oriented partnerships comprised of community members, representatives of government agencies, policymakers, and academic partners. Despite their potential to promote sustainable change, coalitions have had mixed success in effecting long-term improvements in community health. There is a need to assist them in developing strategies for improving and sustaining their functionality. The purpose of this study was to improve understanding of the elements of coalition success and sustainability that are vital to health-related community-based participatory research.

Methods: Although the literature describes coalition functions for effecting sustainable programs and policies, most research reports …

Effects Of A Brief Relaxation Intervention On Stress-Related Eating, Laura Lynn Mayhew-Purcell

USF Tampa Graduate Theses and Dissertations

The experience of stress may contribute to increased food consumption and selection of unhealthy food options. Resource depletion theory suggests stress temporarily depletes resources needed to regulate behavior. Depletions of self-control may result in subsequent failure to regulate eating behaviors, which is particularly salient in restrained eaters. Restraint theory posits people high in dietary restraint require significant effort to control eating. Emotional eating theory further suggests palatable foods may be used to regulate emotional stress reactions. Relaxation exercises to mitigate stress reactions are recommended in eating and weight management programs, but lack quality scientific support. The current study examined the …

Ecological Sanitation In Uganda: Promotion Through Demonstration Facilities And Potential For Ascaris Reduction By Free Ammonia Inactivation Using Stored Urine, John Thomas Trimmer

USF Tampa Graduate Theses and Dissertations

As Uganda works to transform itself into an industrialized, middle-income country in the coming decades, the country is faced with a number of problematic trends that could hinder this transition. High population growth and urbanization are quickly forcing small towns to deal with issues of limited space and the aesthetic conditions within sanitation systems, while declining soil fertility in surrounding rural areas calls into question the future nutritional security of the growing population. Ecological Sanitation (Eco-San) systems, which are designed to recover nutrients from human excreta, may help to address these trends.

Improved sanitation coverage in Uganda is currently estimated …

Multiple Stain Histology Of Skeletal Fractures: Healing And Microtaphonomy, John Wellington Powell

USF Tampa Graduate Theses and Dissertations

The forensic examination of wounds is one of the key elements of analysis performed by forensic anthropologists and forensic pathologists. Gross examination and histological analysis can be used to determine the timing of the wound and its cause. While forensic pathologists are trained to analyze hard and soft tissue wounds, forensic anthropologists, bioarchaeologists, and paleopathologists, focus on hard tissue. Forensic anthropologists have the added benefit of potentially working with residual soft tissue and would benefit from the incorporation of microscopy techniques that take advantage of the soft tissue to better understand perimortem events. Little research has been published that examines …

Exploring The Role Of Intracellular Aminopeptidases In Staphylococcus Aureus Pathogenesis, Devon Nicole Marking

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus is a remarkably pathogenic bacterium that is widely prevalent among the human population. It is the leading agent of skin and soft tissue infections, and is also responsible for causing an array of severe and life threatening diseases. The invasiveness of the pathogen, coupled with increasing antibiotic resistance seen for S. aureus infections, makes this bacterium a prominent public health concern. The extended pathogenicity of S. aureus is largely due to its repertoire of virulence factors, which are typically characterized by being bound to the cell wall, or secreted into the extracellular environment. Previously, our lab identified a …

Evaluation Of School Vip Latrines And User Preferences And Motivations For Adopting Communal Sanitation Technologies In Zwedru, Liberia, Sarah J. Ness

USF Tampa Graduate Theses and Dissertations

This thesis has three objectives as follows: 1) to investigate VIP latrine design and establish if the communal school VIP latrines located on the shared campus of Tubman Wilson Institute (TWI) Junior and Senior High School and J.C. Barlee Elementary School were properly designed and constructed, 2) to explore the user preferences and motivations impacting the adoption of these school latrines, and 3) to develop a framework for factors that influence latrine adoption. These goals were formed by the author in response to her Peace Corps experience working at a high school in Zwedru, Liberia from August 2012 to August …

Examination Of Possible Protective Effect Of Rhesus D Positive Blood Factor On Toxoplasma-Related Depressive Symptoms In Pregnancy, Lisa Lynn Parnell

USF Tampa Graduate Theses and Dissertations

Toxoplasma gondii infects approximately one third of the population worldwide. There is strong evidence that a relationship between T. gondii titer and depressive symptoms exists. There is also evidence suggesting a protective effect of RhD positive blood factor on toxoplasma-induced behavioral and personality changes. This protective effect may influence the relationship between T. gondii and prenatal depressive symptoms. The purpose of this secondary data analysis was to examine the possible protective effect of RhD positive blood factor on prenatal depressive symptoms in 56 pregnant women with T. gondii infection. The cross-sectional design was utilized to answer the question “Does positive …

Development Of A Reusable Nutrient Recovery System To Sustainably Integrate Small-Scale Sanitation And Agriculture, Jorge Luis Calabria

USF Tampa Graduate Theses and Dissertations

Emerging technologies in sanitation and food production foster the potential to advance progress towards key sustainable development goals of ensuring adequate sanitation and food security to all. Non-Sewered Sanitation Systems (NSSSs), as detailed by the recent International Organization for Standardization’s (ISO) 30500 standard, are being developed to overcome challenges of lacking sanitation infrastructure and resource scarcity by providing autonomous, off-grid wastewater treatment (WWT) service in a much smaller footprints than large centralized treatment facilities. A promising NSSS platform is the anaerobic membrane bioreactor (AnMBR) that can achieve high (WWT) throughput in a small footprint using ultrafiltration (UF) membrane filtration to …

Muscling Consumers To Optimal Option Differentiation: The Influence Of Incidental Muscular Sensations On Option Differentiation, Courtney Szocs

USF Tampa Graduate Theses and Dissertations

Marketers often extend product lines by introducing slight variations of existing products (e.g., there are 53 varieties of Crest toothpaste, 15 varieties of Cheerios). As a result, consumers select from assortments containing relatively similar options. Unfortunately, consumers sometimes fail to differentiate among options, and instead consider the different options as similar and choose. Consequently, prior research shows that selecting from choice sets containing relatively similar options can sometimes lead to negative consequences such as decreased satisfaction. In light of these negative consequences, and given the frequency with which consumers choose from sets of similar options, it becomes important to identify …

Quantification Of Protein Adhesion Strength To Surface Attached Poly (N- Isopropylacrylamide) Networks By Hydrodynamic Detachment Shear Stresses, Gulnur Sanden

USF Tampa Graduate Theses and Dissertations

Stimuli responsive coatings offer a versatile method by which to manipulate interfacial interactions of proteins in a desired way. However, there exists little guidance as to how the structure of a responsive polymer coating influences adsorption of proteins. In this dissertation, the adsorption behavior of immuglobulin G (IgG) on poly (N-isopropylacryamide) (PNIPAAm) hydrogel coatings was investigated as a function of film thickness. PNIPAAm exhibits a hydrophilic to hydrophobic transition above a critical temperature of ~32°C in aqueous solutions. In this research, through the use of quartz crystal microbalance with dissipation (QCM-D) it was observed that the adsorption was thickness dependent …

Perceptions Of Practitioners In A Voluntary Prekindergarten On Probation, Susan Evelyn Weber

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to describe and explain the perspectives and beliefs that practitioners have about prekindergarten in a Voluntary Prekindergarten program unable to meet minimal accountability requirements. The exploratory questions included: What are the beliefs and perceptions about effective and appropriate early childhood education among practitioners in a Voluntary Prekindergarten program on probation? And in what ways does probation influence practitioners' perceptions and beliefs about early childhood education? I used participants from a school where I conducted a pilot study. My data collection consisted of semi-structured interviews, resumes, my research reflective journal, and the centers program improvement …

Medicaid Pays For That? An Exploratory, Mixed-Methods Analysis Of Florida Home Birth, Nicole K. Demetriou

USF Tampa Graduate Theses and Dissertations

The overwhelming social norm for pregnant women in the U.S. is to receive prenatal care from an obstetrician and to give birth in a hospital setting. However, the incidence of midwifery care and out-of-hospital birth is increasing, particularly among White, non-Hispanic women. Florida has been considered a "model" state for home birth midwifery given legislative support that mandates coverage of all types of midwifery (e.g., Certified Professional Midwives and Certified Nurse-Midwives) care in all birth settings (e.g., hospital, home, birth center) and by all forms of insurance (e.g., commercial and Medicaid). Medicaid is the payer source for nearly half of …

Wastewater Nutrient Recovery Using Anaerobic Membrane Bioreactor Permeate For Hydroponic Fertigation, Jorge Luis Calabria

USF Tampa Graduate Theses and Dissertations

The imbalance between global population growth and resource consumption is indicative of unsustainable practices and foreshadows a grim future of continued resource depletion, food and water scarcity, social inequality, and deteriorating public and environmental health. Meanwhile, the urban centers of the world continue to experience exponential growth resulting in overwhelmed food, water, and sanitation infrastructure. Decentralized and satellite wastewater treatment technologies capable of resource recovery, such as anaerobic membrane bioreactors (AnMBR), foster synergistic opportunities to help manage the food, energy, and water sectors of urban environments. Specifically, the nutrient concentration and high effluent quality of permeate produced by AnMBR systems …

Differentiating Geo-Spatiotemporal Aquatic Larval Habitats Of Anopheles Gambiae Complex In Urban Agriculture And Urban Non-Agriculture Environments In Accra, Ghana, Samia Mckeever

USF Tampa Graduate Theses and Dissertations

To meet the rising food demands of communities in Accra,Ghana, urban agriculture has been popularized as a way to increase food security and improve nutrition (Donovan et al., 2012). Urban agriculture is defined as "the cultivation of crops at both the subsistence and commercial levels including the keeping of livestock in open spaces in urban areas (Adjaye, n.d.). In Accra, urban agriculture covers 1,091 hectares, employs over one thousand people, and supplies residents with 90% of its vegetables ("Accra Metropolitan", n.d.). Further, 60% of households in Accra participate in backyard farming ("Accra Metropolitan", n.d.). Although urban agriculture provides many benefits …

Perspectives Of Hiv + Women On The Mother To Child Transmission Of Hiv In Addis Ababa, Ethiopia, Kimberly Anne Fleek

USF Tampa Graduate Theses and Dissertations

Purpose and rationale: In 2012, an estimated 9,500 infants in Ethiopia were born with HIV. Mortality for these infants is high, and preventing infection offers the best hope for reducing the childhood death rates. Effective measures exist which can reduce the likelihood of a child acquiring HIV from its mother to less than 2%, and the necessary anti-retroviral medications are free and accessible to Ethiopian women. However, Prevention of Mother to Child Transmission of HIV (PMTCT) efforts in the country have not kept pace with the global reduction in infant infections over the last decade, and the Ethiopian MTCT rate …

Understanding Factors Determining Early Termination From A Government Assistance Program For Maternal And Child Health: The Special Supplemental Nutrition Program For Women, Infants And Children (Wic), Anthony Dominic Panzera

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation is to understand why individuals enrolled in the Special Supplemental Nutrition Program for Women, Infants and Children (WIC) fail to retrieve food vouchers, miss WIC appointments, and become inactive in program components. In Kentucky, mothers who fail to pick up food instruments for 60 days are automatically terminated from the program. The specific research questions that guided this study are: (1) Which segments of enrollees are at greatest and least risks of nonparticipation in the WIC program? (2) How do predisposing, enabling and need characteristics impact WIC nonparticipation among eligible mothers? (3) How do WIC …

"Having Our Say": Exploring The Processes And Feasibility Of A Community-Based Participatory Intergenerational Physical Activity Program For Grandparents Raising Grandchildren, Tiffany Lenell Young

USF Tampa Graduate Theses and Dissertations

Over the past twenty years, the number of grandparents raising grandchildren has increased substantially. In many cases, raising grandchildren can be stressful and may aggravate pre-existing health conditions. Grandchildren in these kinship relationships often experience poor health outcomes as well. Typically, both grandparents and grandchildren do not engage in positive health behaviors. Thus, there is a need to develop intergenerational health promotion interventions for grandparents raising grandchildren. This study used the community-based participatory research approach to develop and implement an eight-week intergenerational program for kinship families. The specific goals of this descriptive study were to understand the process and feasibility …

"Wake Up The Knowledge That You Have": An Assessment Of Community Food Security In Fellsmere, Florida, Susan Marie Tyler

USF Tampa Graduate Theses and Dissertations

In the global industrial agricultural system many people lack access to high-quality nutritious foods and food production techniques are often inefficient and reliant on harsh chemical inputs. While numerous strategies exist to address the disparities present in the global food system, increasingly researchers and practitioners are looking to local food systems for solutions to strengthen community food security (CFS). CFS emphasizes small-scale production strategies such as farmer's markets, community gardens, and consumer supported agriculture. As these efforts evolve, research is needed to understand how these strategies affect communities. To explore a local CFS initiative, qualitative data were collected from community …

Can Anyone With Low Income Be Food Secure?: Mitigating Food Insecurity Among Low Income Households With Children In The Tampa Bay Area, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

In the US over the last few years, approximately 14.5% of households experience food insecurity at some point throughout the year. While studies on food insecurity in the US have determined that household income and specifically income available to spend on food is of critical importance to food security, it is still unclear why some households with low income are able to maintain food security while others experience food insecurity in a pattern characterized as not constant but recurrent. This dissertation compares households with children at different levels of food security and insecurity using the USDA Core Food Security Module …

Are Gopher Tortosies (Gopherus Polyphemus Daudin) Comapatible With Cows?, Thomas William Hentges

USF Tampa Graduate Theses and Dissertations

Many Gopher Tortoise populations are in steep decline throughout Florida, and various measures have been attempted to curb the trend. One such measure is to relocate tortoises to protected recipient sites on private lands. The majority of private lands in Florida are used for cattle, however, production and the effect of cattle production on tortoises is not known. Here, I tested six parameters of tortoise behavior by monitoring 1403 gopher tortoises released at the Barthle Brothers Ranch, Pasco County Florida, between August 2009 and December 2012. The parameters tested were (1) burrow density, (2) burrow spacing, (3) burrow relocation, (4) …

Use Of System Dynamics Modeling To Explicate The Theory-Of-Change Of A Social Marketing Innovation, Brian J. Biroscak

USF Tampa Graduate Theses and Dissertations

Community coalitions are an important part of the public health milieu and thus subject to many of the same external pressures as public health organizations--including changes in required strategic orientation. Many funding agencies have shifted their funding agenda from program development to policy change. Thus, the Florida Prevention Research Center created the Community-Based Prevention Marketing for Policy Development framework to teach community coalitions how to apply social marketing to policy change. The dissertation research reported here was designed to explicate the framework's theory-of-change. The research question was: "What are the linkages and connections between CBPM inputs, activities, immediate outcomes, intermediate …