Digital Commons Network^™

Carbon Nanotube Enhanced Maldi Ms: Increasing Sensitivity Through Sample Concentration, Joshua Schumacher

USF Tampa Graduate Theses and Dissertations

Matrix-assisted laser desorption/ionization (MALDI) is a technique used in mass spectrometry for the ionization of biomolecules. A matrix solution is mixed with the analyte molecules to be investigated, and then spotted onto a specialized MALDI plate. The solvents evaporate leaving only the re-crystallized matrix with analyte dispersed throughout the crystals. Sample ionization is accomplished with a laser in the MALDI instrument. The spot diameter of the target is usually several orders of magnitude larger than the diameter of the laser, making it necessary to perform multiple laser investigations to accurately evaluate the analyte in the target spot.

Experiments were performed …

Examination Of The Effect Of Child Abuse Case Characteristics On The Time A Caseworker Devotes To A Case, Christopher J. Card

USF Tampa Graduate Theses and Dissertations

This study used an explanatory research model that determined the effect on caseworker time and therefore workload caused by specific characteristics of cases assigned after the child abuse investigation is complete. The purpose of this study was to explain the relationship between child protection case characteristics and the time an assigned caseworker devotes to a case. With this knowledge an informed methodology to assess the current workload of a caseworker could be used to assure that the caseworker is able to successfully complete the tasks required for each child assigned. Further, the knowledge of the amount of time spent on …

Understanding The Interaction Between Blood Flow And An Applied Magnetic Field, Francy L. Sinatra

USF Tampa Graduate Theses and Dissertations

Hemodynamic monitoring is extremely important in the accurate measurement of vital parameters. Current methods are highly invasive or noncontinuous, and require direct access to the patient’s skin. This study intends to explore the modulated magnetic signature of blood method (MMSB) to attain blood flow information. This method uses an applied magnetic field to magnetize the iron in the red blood cells and measures the disturbance to the field with a magnetic sensor [1]. Exploration will be done by experimentally studying in-vitro, as well as simulating in COMSOL the alteration of magnetic fields induced by the flow of a magnetic solution. …

Characterization Of The Role Of Nicotine And Delta 9-Thc In Modulation Of Neuroinflammation, Jared C. Ehrhart

USF Tampa Graduate Theses and Dissertations

Neuroinflammation is a major driving force in the progression of neurodegenerative disorders. Nicotinic acetylcholine receptors, as well as cannabinoid CB2 receptors, have been shown to have strong anti-inflammatory properties when activated. These effects are shown, in vivo, to be a result of stimulation of α7 nAChRs and CB2 cannabinoid receptors. Microglia cells, an immune cell in the brain, are shown to express both of these receptor subtypes. The studies detailed herein, investigated the ability of two compounds, nicotine and Δ9-THC, in modulation of inflammatory processes. Stimulation of these receptors on microglia using nicotine and Δ9-THC blocked the activation of these …

Consumable Process Development For Chemical Mechanical Planarization Of Bit Patterned Media For Magnetic Storage Fabrication, Joseph T. Bonivel Jr.

USF Tampa Graduate Theses and Dissertations

As the superparamagnetic limit is reached, the magnetic storage industry looks to circumvent the barrier by implementing patterned media (PM) as a viable means to store and access data. Chemical mechanical polishing (CMP) is a semiconductor fabrication technique used to planarize surfaces and is investigated as a method to ensure that the PM is polished to surface roughness parameters that allow the magnetic read/write head to move seamlessly across the PM. Results from this research have implications in feasibility studies of utilizing CMP as the main planarization technique for PM fabrication. Benchmark data on the output parameters of the CMP …

Ballistic Penetration Of A Sandbagged Redoubt Using Silica Sand And Pulverized Rubber Of Various Grain Sizes, Robert Paul Cole

USF Tampa Graduate Theses and Dissertations

The basis of this work is to find how varying the grain size of materials contained in sandbags (sand and crumb rubber) effects the ballistic penetration of the projectiles from both the 7.62x39mm (308-short), and 9mm Luger cartridges. The sandbags were stacked in a pyramidal stacking configuration according to military specifications in order to simulate a section of a sandbag barrier or redoubt as would be seen on the battlefield. The projectiles were fired at the targets, and the velocity and penetration data was recorded. The results concern both military and civilian applications alike. The 7.62x39 round was found to …

Design And Fabrication Of A Membrane Integrated Microfluidic Cell Culture Device Suitable For High-Resolution Imaging, Alla A. Epshteyn

USF Tampa Graduate Theses and Dissertations

Malaria remains a serious concern for people living and traveling to warm climates in Africa, Asia, and some parts of America. Understanding the mechanism of the malaria parasite in the liver phase could lead to important discoveries for preventative and treatment therapeutics before the disease develops into the blood stage. While in vitro liver cell culture models have been explored, a device that mimics the liver cell architecture with the capability of high-resolution imaging has never been created. In this research, a cell culture microfluidic device was designed and fabricated with a membrane integrated design to mimic the architecture of …

The Use Of Proteomic Techniques To Study The Physiology And Virulence Of Staphylococcus Aureus, Frances Rivera

USF Tampa Graduate Theses and Dissertations

Staphylococcus aureus

is a bacterial pathogen that is believed to be the most common agent of human infectious disease, causing conditions ranging from common skin lesions to life-threatening illnesses. S. aureus has also shown a remarkable ability to develop resistance to antimicrobial treatment, making infections difficult to treat. In the post-genomic era, proteomic studies analyzing the protein complement of a genome in a particular organism at any given time, have gained real significance. This result is largely due to dynamic changes in protein expression profiles which can lead wide alterations in physiology and behavior. For proteomics, it is necessary to …

From Night To Dawn: The Cultural Criticism Of George A. Romero, Christopher Paul Wagenheim

USF Tampa Graduate Theses and Dissertations

Analyzing George A. Romero's Night of the Living Dead (1968) and Dawn of the Dead (1978) in relation to the early works of Marshal McLuhan, Erich Fromm, and Herbert Marcuse reveals an ideological parallel that can be explicated using Antonio Gramsci's theory of cultural hegemony. While McLuhan, Marcuse, and Fromm observe, in order to critique, social manifestations of power in a consumerist system, Romero presents a model of hegemony in his films that he exposes to extreme stress thereby allowing viewers to observe such manifestations of power for themselves. These analyses are significant because although Marcuse, McLuhan, Fromm, and Romero …

Investigating The Mode Of Action Of A Novel N-Sec-Butylthiolated Beta-Lactam Against Staphylococcus Aureus, Katherine Rose Prosen

USF Tampa Graduate Theses and Dissertations

N-sec

-butylthioloated β-lactam (NsβL) is a novel beta-lactam antimicrobial with a mechanism of action proposed to inhibit 3-oxoacyl-acyl carrier protein synthase (ACP) III (FabH), resulting in the inhibition of fatty acid synthesis. It has been suggested that NsβL inhibits FabH indirectly by inactivating coenzyme-A (CoA). CoA is an essential cofactor for numerous proteins involved in glycolysis, the citric acid cycle (TCA), and pyruvate metabolism, in addition to fatty acid biosynthesis. This study aimed to determine the effects of NsβL on a diverse array of laboratory and clinical Staphylococcus aureus isolates by analyzing the mode of resistance in spontaneous and adaptive …

The Green And Ampt Infiltration Model Accounting For Air Compression And Air Counterflow In The Shallow Water Table Environment: Laboratory Experiments, Yuliya Lukyanets

USF Tampa Graduate Theses and Dissertations

Water infiltration into the unsaturated zone especially in a shallow water table environment is affected by air compression ahead of the wetting front and air counterflow. Neglecting air compression in infiltration modeling can overestimate infiltration and infiltration rates, whereas not accounting for air counterflow can underestimate infiltration and infiltration rates due to unrealistic buildup of air pressure resistance ahead of the wetting front. A method, derived on the basis of the Green and Ampt (1911) infiltration model, is introduced to simulate air compression and air counterflow during infiltration into a shallow water table. The method retains the simplicity of the …

News Media Representations Of Women In The U.S. Military Post September 11, 2001, Colleen Krepstekies

USF Tampa Graduate Theses and Dissertations

This paper examines newspaper portrayals produced by the

Washington Post, the New York Times, and the Los Angeles Times of women in the military from Sept. 11, 2001, to Sept. 11, 2009. The purpose was to identify how the three nationally recognized U.S. newspapers depict women’s expanding combat roles on contemporary battlefields that lack definitive front lines. Because the news media are the primary vehicle to update the general public on military matters, how the news media portray military women can play a role in shaping audience perceptions of military women. In turn, this relationship can influence the …

Molecular Detection Of The Toxic Marine Diatom Pseudo-Nitzschia Multiseries, Jennifer A. Delaney

USF Tampa Graduate Theses and Dissertations

The marine diatom genus Pseudo-nitzschia includes species that produce domoic acid, a neurotoxin responsible for illness and mortality in both humans and marine wildlife. Because of the expertise and time required for the microscopic discrimination of species, molecular methods that monitor environmental concentrations of Pseudo-nitzschia provide a rapid alternative for the early detection of blooms and prediction of toxin accumulation. We have developed a nucleic acid sequence-based amplification with internal control RNA (IC-NASBA) assay and a quantitative reverse transcription PCR (qRT-PCR) assay for the detection of the toxic species P. multiseries targeting the ribulose- 1,5-biphosphate carboxylase/oxygenase small subunit (rbcS) gene. …

Contemporary Afro-Cuban Voices In Tampa: Reclaiming Heritage In “America’S Next Greatest City”, Linda M. Callejas

USF Tampa Graduate Theses and Dissertations

This dissertation presents findings from ethnographic research conducted with members of the Sociedad La Unión Martí-Maceo, established by segregated Black Cuban cigar workers in Ybor City in 1904. For decades, Tampa officials have initiated numerous urban revitalization projects aimed at developing a world-class tourist destination and metropolitan center. Often, these efforts have centered on highlighting the ethnic history of Ybor City, from which the participation of Black Cubans and the Martí-Maceo Society have been actively excluded or ignored. The main issues related to contemporary Afro- Cuban identity in Tampa and which will be examined in my dissertation, include the changing …

Body Depilation Among Women And Men: The Association Of Body Hair Reduction Or Removal With Body Satisfaction, Appearance Comparison, Body Image Disturbance, And Body Dysmorphic Disorder Symptomatology, Michael Scott Boroughs

USF Tampa Graduate Theses and Dissertations

Body depilation, or the reduction or removal of body hair, is a relatively new area of research inquiry. Although women in many industrialized cultures have engaged in depilation for several decades, this behavior has been documented only recently among men. Though originally thought to be widely practiced by women and only a small proportion of men, including athletes or bodybuilders, recent studies suggest that more men engage in body depilation than previously hypothesized. In fact, one recent study estimated the prevalence of men's body depilation at 83.7% which suggests that men are depilating at rates similar to women. Nevertheless sparse …

N-Glycosylation Modulates Gating And Antibiotic Block Of The Human Potassium Channel, Herg1a, Sarah A. Norring

USF Tampa Graduate Theses and Dissertations

Arrhythmias are often caused by aberrant ion channel activity, resulting in remodeling of the cardiac action potential. Two K

+ currents, IKs and IKr, contribute to phase III repolarization of the human cardiac action potential. Human ether-a-go-go-related gene 1 (hERG1), a voltage-gated potassium channel, underlies IKr. Alterations in the repolarization phase of the action potential, and in particular IKr, can lead to arrhythmias, long or short QT syndrome, heart disease, and sudden cardiac death. HERG1A has two putative N-glycosylation sites located in the S5-S6 linker region, one of which is N-glycosylated. The aim of the first study was to …

A Microwave Radiometer For Close Proximity Core Body Temperature Monitoring: Design, Development, And Experimentation, Quenton Bonds

USF Tampa Graduate Theses and Dissertations

Presented is a radiometric sensor and associated electromagnetic propagation models, developed to facilitate non-invasive core body temperature extraction. The system has been designed as a close-proximity sensor to detect thermal emissions radiated from deep-seated tissue 1 cm – 3 cm inside the human body. The sensor is intended for close proximity health monitoring applications, with potential implications for deployment into the improved astronaut liquid cooling garment (LCG). The sensor is developed for high accuracy and resolution. Therefore, certain design issues that distort the close proximity measurement have been identified and resolved. An integrated cavity-backed slot antenna (CBSA) is designed to …

The Emotional Guardianship Of Foreign-Born And Native-Born Hispanic Youth And Its Effect On Violent Victimization, Amy Sheena Eggers

USF Tampa Graduate Theses and Dissertations

This study seeks to expand the scope of assimilation theory by integrating it with elements of routine activities theory to better understand what influence assimilation has in regard to violent victimization. Specifically, the purpose of this study is to determine whether or not differences in victimization rates between foreign-born and native-born Hispanic youth are related to variations in emotional guardianship. Emotional guardianship refers to the aspect of relationships (i.e., affection and communication) between Hispanic youth and their parents that serve to protect the youth from being victimized. I hypothesize that foreign-born Hispanics have greater emotional guardianship than native-born Hispanics, and …

A Case Study Of The Significant Events And Legal Parameters Surrounding Charter School Movement At The State And Federal Level, Monica L. Ilse

USF Tampa Graduate Theses and Dissertations

All states have different perspectives and various statutes within broader constitutional law. Perception of public dissatisfaction with public schools has led to choice schooling options for parents. One of the fastest growing choice options in schooling is charter schools; schools privately run by organizations through public funds. This study analyzes the governance of charter schools and how charters operate under legal guidelines and Florida statutes, with significant legislative events cited. This study answers the following questions as they relate to evolution and legal parameters surrounding the charter movement using exploratory case study method: 1) What is the evolution of the …

Architecture And Compiler Support For Leakage Reduction Using Power Gating In Microprocessors, Soumyaroop Roy

USF Tampa Graduate Theses and Dissertations

Power gating is a technique commonly used for runtime leakage reduction in digital CMOS circuits. In microprocessors, power gating can be implemented by using sleep transistors to selectively deactivate circuit modules when they are idle during program execution. In this dissertation, a framework for power gating arithmetic functional units in embedded microprocessors with architecture and compiler support is proposed. During compile time, program regions are identified where one or more functional units are idle and sleep instructions are inserted into the code so that those units can be put to sleep during program execution. Subsequently, when their need is detected …

Evolution Of The Hammerhead Cephalofoil: Shape Change, Space Utilization, And Feeding Biomechanics In Hammerhead Sharks (Sphyrnidae), Kyle Reid Mara

USF Tampa Graduate Theses and Dissertations

The relationship between form and function is often used to elucidate the biological role of a structure. Hammerhead sharks offer a unique opportunity to study form and function through phylogeny. Because sphyrnid sharks display a range of cranial morphologies this group can be used to address questions about the evolution of cranial design and investigate the effects of changes in head morphology on feeding structures and bite force. Geometric morphometrics, volumetric analyses, morphological dissections, and phylogenetic analyses of the cephalofoil were used to gain insight into changes in cranial design through evolutionary history. External morphometrics and internal volumetric analyses indicated …

Applications Of Molecular Dynamics Techniques And Spectroscopic Theories To Aqueous Interfaces, Anthony Green

USF Tampa Graduate Theses and Dissertations

The primary goal of spectroscopy is to obtain molecularly detailed information about the system under study. Sum frequency generation (SFG) vibrational spectroscopy is a nonlinear optical technique that is highly interface specific, and is therefore a powerful tool for understanding interfacial structure and dynamics. SFG is a second order, electronically nonresonant, polarization experiment and is consequently dipole forbidden in isotropic media such as a bulk liquid. Interfaces, however, serve to break the symmetry and produce a signal. Theoretical approximations to vibrational spectra of O-H stretching at aqueous interfaces are constructed using time correlation function (TCF) and instantaneous normal mode (INM) …

Utilizing Facebook Application For Disaster Relief: Social Network Analysis Of American Red Cross Cause Joiners, Jennie Wan Man Lai

USF Tampa Graduate Theses and Dissertations

With the exponential growth of Facebook users worldwide, this platform for social network online has become a powerful tool to connect individuals and share information with each other. This study explores the phenomenal trend of utilizing a Facebook application called Causes to help users organize into online communities for a specific cause and mobilize their resources for disaster relief during the Haiti earthquake disaster. Two separate samples of 100 joiners each from the American Red Cross (ARC) Cause on Facebook were randomly selected before and after the Haiti earthquake disaster to examine the differences of the composition (i.e., attributes) and …

The Impact Of Maladaptive Schema On Disordered Eating: A Collective Case Study, Susan Hurley

USF Tampa Graduate Theses and Dissertations

This qualitative study is based on the reality that disordered eating such as anorexia nervosa, bulimia nervosa, and compulsive overeating resulting in obesity represent a major and growing problem in community health. Treatment models using cognitive behavioral therapy suggest that those diagnosed with an eating disorder tend to judge themselves in terms of their body shape, weight, and eating habits. However, the recovery rate for those treated for an eating disorder that only addresses those three issues identified above is less than 60%. A number of quantitative studies have provided evidence that other maladaptive schema may contribute to bulimic and …

Photothermal Studies Of Carboxymyoglobin, Meagan Small

USF Tampa Graduate Theses and Dissertations

Small ligand diffusion in heme proteins is not fully understood. To help better understand CO diffusion, three systems were investigated: L29H/F43H site-directed sperm whale myoglobin, horse heart myoglobin in a heavy water buffer, and calix[4]resorcinarene. Binding of copper to calix[4]resorcinarene was photophysically characterized to unravel transient binding of small molecules in heme-copper proteins. Copper binding was found to have a low dissociation constant of approximately 8.6 micrometers.. Reaction profiles using photoacoustic calorimetry were constructed for the myoglobin systems. In deuterium oxide, ligand escape is not rate limited by water entry. Large enthalpy differences arise from the thermodynamic properties of deuterium …

Investigation Of Buildup Dose For Therapeutic Intensity Modulated Photon Beams In Radiation Therapy, Khosrow Javedan

USF Tampa Graduate Theses and Dissertations

Buildup dose of Mega Voltage (MV) photon beams can be a limiting factor in intensitymodulated radiation therapy (IMRT) treatments. Excessive doses can cause patient discomfort and treatment interruptions, while underdosing may lead to local failure.

Many factors which contribute to buildup dose, including the photon beam energy spectrum, scattered or contaminant radiation and their angular distribution, are not modeled well in commercial treatment planning systems. The accurate Monte Carlo method was employed in the studies to estimate the doses.

Buildup dose of 6MV photon beams was investigated for three fundamentally different IMRT modalities: between Helical TomoTherapy and traditional opposed tangential …

Computer Simulation Of Metal-Organic Materials, Abraham C. Stern

USF Tampa Graduate Theses and Dissertations

Computer simulations of metal-organic frameworks are conducted to both

investigate the mechanism of hydrogen sorption and to elucidate a detailed,

molecular-level understanding of the physical interactions that can lead to successful

material design strategies. To this end, important intermolecular interactions are

identified and individually parameterized to yield a highly accurate representation

of the potential energy landscape. Polarization, one such interaction found to play a

significant role in H

2 sorption, is included explicitly for the first time in simulations

of metal-organic frameworks. Permanent electrostatics are usually accounted for by

means of an approximate fit to model compounds. The application of …

Characterization Of Unidentified Viruses From Florida, Jessie L. Dyer

USF Tampa Graduate Theses and Dissertations

Public Health and clinical laboratories occasionally obtain viral isolates that cannot be typed by routine methods. Therefore, the sequence-independent, single primer amplification (SISPA) technique was adapted to rapidly identify and characterize viral isolates of unknown etiology. A panel of known (West Nile virus and St. Louis encephalitis virus) and unknown viral isolates (environmental samples collected in Florida) were used to develop and refine the SISPA technique. Selectivity for viral genomic sequences was obtained through enriching viral particles by centrifugation, removal of cellular debris by filtration and removal of host genomic material by benzonase application. The SISPA method successfully amplified the …

The Positive- And Negative-Right Conceptions Of Freedom Of Speech And The Specter Of Reimposing The Broadcast Fairness Doctrine ... Or Something Like It, Adam Fowler

USF Tampa Graduate Theses and Dissertations

A key theoretical debate underlying the now defunct Federal Communications Commission (FCC) regulation known as the Fairness Doctrine is conflict over what constitutes the right to freedom of speech: a positive or negative conception. Similarly, since repeal of the Doctrine, other FCC measures to uphold the “public-interest” standard in broadcasting have relied on a positive conception of speech. This thesis demonstrates the history of this debate through court cases, news reports, scholarly articles and historical documents. It then is argued that the positive-right nature of these regulations is problematic philosophically, constitutionally and practically. The positive-right conception lends itself to an …

Identifying And Addressing Health Disparities In Black Older Adults With Osteoarthritis, Chivon A. Mingo

USF Tampa Graduate Theses and Dissertations

Osteoarthritis (OA), the most common form of arthritis in older adults, often results in pain, disability and poor psychological well-being. Compared to White adults, Black adults consistently report more pain, more activity limitations, and have different perceptions about OA. Racial disparities also exist in treatments, and prevalence of arthritis. It is imperative to have effective interventions and treatment options for older Blacks. Yet, few arthritis interventions have included Black participants in their samples, and nearly all of those have failed to report separate analyses indicating the effectiveness for Black adults, thus leaving a gap in the literature. The purpose of …