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More Than "Modern Day Slavery": Stakeholder Perspectives And Policy On Human Trafficking In Florida, Nathaniel Dickey

USF Tampa Graduate Theses and Dissertations

In recent years, Florida has acquired a reputation as fertile ground for human trafficking. On the heels of state and federal anti-human trafficking legislation, a host of organizations have risen to provide a range of services. In this thesis, I discuss findings from 26 interviews conducted with law enforcement, service providers, legal representatives and trafficked persons to contextualize the variability in the way anti-trafficking work is conceptualized by stakeholders across the state. Additionally, I explore how conflicting organizational policies on the local, state, and federal levels impact stakeholder collaboration and complicate trafficked persons' attempts to navigate already complex processes of …

Development Of A Non-Invasive Electrode For Intradermal Electrically Mediated Dna Vaccination, Amy Lynn Donate

USF Tampa Graduate Theses and Dissertations

Current progress in the development of vaccines has decreased the incidence of fatal and non-fatal infections and increased longevity. However, new technologies need to be developed to combat an emerging generation of infectious diseases. DNA vaccination has been demonstrated to have great potential for use against a wide variety of diseases. Alone, this vaccine technology does not generate a significant immune response for vaccination, but combined with delivery by electroporation (EP), can enhance plasmid expression and immunity against the expressed antigen. Most EP systems, while effective, can be invasive and painful making them less desirable for use in vaccination. Our …

A Framework For Determining The Reliability Of Nanoscale Metallic Oxide Semiconductor (Mos) Devices, Wilkistar Otieno

USF Tampa Graduate Theses and Dissertations

An increase in worldwide investments during the past several decades has pro-pelled scienti c breakthroughs in nanoscience and technology research to new and exciting levels. To ensure that these discoveries lead to commercially viable prod-ucts, it is important to address some of the fundamental engineering and scientific challenges related to nanodevices. Due to the centrality of reliability to product integrity, nanoreliability requires critical analysis and understanding to ensure long-term sustainability of nanodevices and systems. In this study, we construct a relia-bility framework for nanoscale dielectric lms used in Metallic Oxide Semiconductor (MOS) devices. The successful fabrication and incorporation of metallic …

Dna Methylation And Its Association With Prenatal Exposures And Pregnancy Outcomes, Jennifer Straughen

USF Tampa Graduate Theses and Dissertations

Altered DNA methylation may lead to suboptimal fetal programming, increasing the risk of adverse pregnancy outcomes such as small for gestational age (SGA); however, few studies have examined the associations between DNA methylation, prenatal exposures, and fetal outcomes. Cross-sectional data from a larger, ongoing study were used to assess the impact of prenatal smoking on gene specific methylation of umbilical cord blood derived DNA and to investigate the association between gene-specific methylation and risk of SGA. The association between gene-specific DNA methylation and birthweight was also assessed. Maternal and infant covariates were abstracted from medical records, cigarette smoke exposure was …

Epidemiological Study Of Contributing Factors In The Development Of Peptic Ulcer And Gastric Cancer Initiated By Helicobacter Pylori Infection In India, Rahul Suresh Mhaskar

USF Tampa Graduate Theses and Dissertations

Background: Helicobacter pylori (H. pylori) infection is a significant risk factor for peptic ulcer (PU) and gastric cancer (GC). Apart from the virulent CagA genotype of H. pylori environmental and dietary factors influence disease outcomes. There have been no studies addressing these factors in Western India. Hence, we conducted a case control study enrolling PU, GC patients and controls at Pune, India.

Methods: Risk factors for PU and H. pylori infection were assessed in participant interview. H. pylori status was assessed from stool by monoclonal antigen detection. To understand treatment effect, we followed 100 H. pylori positive patients.

Results: We …

African American Athletes And The Negotiation Of Public Spaces: An Examination Of Athletic Capital And African American Perceptions Of Success, Keona Lewis

USF Tampa Graduate Theses and Dissertations

This dissertation explores the culture of sport among African American male football players as well as African American perspectives on sport and success. A case study of six African American, Division 1 FBS (Football Bowl Subdivision) collegiate student athletes was conducted along with seventeen supplemental interviews with community members, parents, coaches and former athletes and fans. The participants answered questions that explored education, success, identity construction, ethnicity and sport. Archival data was also reviewed framing the discussion on football in Florida, links between education and sport participation and African American male academic achievement. While many perspectives varied, there were collective …

The Roles Of Elevated Bcl-2 In Ovarian Cancer, Nicole Shree Anderson

USF Tampa Graduate Theses and Dissertations

Ovarian cancer (OC) is the second most common gynecologic cancer; however it is responsible for the most gynecologic cancer-related deaths. Apoptosis evasion is an important mechanism in OC tumorigenesis, and the prototypic anti-apoptotic protein, B-cell lymphoma 2 (Bcl-2), is often overexpressed in OC tumors. Gaining a better understanding of the mechanism(s) behind Bcl-2 overexpression and potential extra-anti-apoptotic functions of Bcl-2 could elucidate the importance of elevated Bcl-2 in OC. In the current study, I show through immunohistochemical analysis of normal, benign, and OC tissue sections, that both epithelial and stromal Bcl-2 expression decreases with OC progression. However, the number of …

Before The Storm: Evacuation Intention And Audience Segmentation, Homer J. Rice

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to describe the predictors of evacuation intention among coastal residents in the State of Florida and to determine if there are meaningful segments of the population who intend to evacuate when told to do so by governmental officials because of a major hurricane. In the America’s and the Caribbean, 75,000 deaths have been attributed to hurricanes in the 20

th century. A well planned evacuation can reduce injury and death, yet many people do not have an evacuation plan and do not intend to evacuate when told to do so. The study used secondary …

Chromatin Unfolding By Cdt1 Regulates Mcm Loading Via Opposing Functions Of Hbo1 And Hdac11-Geminin, Philip G. Wong

USF Tampa Graduate Theses and Dissertations

The efficiency of metazoan origins of DNA replication is known to be enhanced

by histone acetylation near origins. Although this correlates with increased MCM

recruitment, the mechanism by which such acetylation regulates MCM loading is

unknown. We show here that Cdt1 induces large-scale chromatin decondensation that is

required for MCM recruitment. This process occurs in G1, is suppressed by Geminin, and

requires HBO1 HAT activity and histone H4 modifications. HDAC11, which binds Cdt1

and replication origins during S-phase, potently inhibits Cdt1-induced chromatin

unfolding and re-replication, suppresses MCM loading, and binds Cdt1 more efficiently

in the presence of Geminin. We also …

Libs And Lite Emission Based Laser Remote Sensing Of Chemical Species And Enhanced Modeling Of Atmospheric Absorption, Dzianis V. Pliutau

USF Tampa Graduate Theses and Dissertations

Laser-Induced Breakdown spectroscopy (LIBS) and Laser-Induced Thermal Emission (LITE) emission based laser remote sensing were investigated with the application to the remote measurements of trace chemical species. In particular, UVvisible LIBS and Mid-IR LITE systems were developed and measurements of remote targets and chemical surfaces were studied. The propagation through the atmosphere of the multi-wavelength backscattered LIBS and LITE optical spectrum with atmospheric absorption effects on the returned lidar signal was investigated. An enhanced model of the atmospheric effects on emission-based laser-remote sensing was developed and found to be consistent and in agreement with our experimental results.

LITE measurements were …

Novel Roles For 185delag Mutant Brca1 In Ovarian Cancer Pathology, Rebecca J. Linger

USF Tampa Graduate Theses and Dissertations

Familial history is the strongest risk factor for developing ovarian cancer (OC), and a significant contributor to breast cancer risk. Most hereditary breast cancers and OCs are associated with mutation of the tumor suppressor Breast and Ovarian Cancer Susceptibility Gene 1 (BRCA1). Studying risk-associated BRCA1 truncation mutations, such as the founder mutation 185delAG, may reveal signaling pathways important in OC etiology. Recent studies have shown novel BRCA1 mutant functions that may contribute to breast and OC initiation and progression independent of the loss of wtBRCA1. Previously, we have found that normal human ovarian surface epithelial (HOSE) cells expressing the 185delAG …

Using Virtual Environments As Professional Development Tools For Pre-Service Teachers Seeking Esol Endorsement, Rebecca J. Blankenship

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate the potential use of Second Life (Linden Labs, 2004) and Skype (Skype Limited, 2009) as simulated virtual professional development tools for pre-service teachers seeking endorsement in teaching English as a Second Official Language (ESOL. Second Life is an avatar-based Internet program that allows end-users to interact, using audio and chat features, with a digital representation of themselves (an avatar). Skype is an Internet-based video conferencing program that allows users to see each other by way of a webcam. Of particular interest to this study was how a group of 12 pre-service teacher …

Evaluation Of Deep Geologic Units In Florida For Potential Use In Carbon Dioxide Sequestration, Tina Roberts-Ashby

USF Tampa Graduate Theses and Dissertations

Concerns about elevated atmospheric carbon dioxide (CO

2

) and the effect on

global climate have created proposals for the reduction of carbon emissions from large

stationary sources, such as power plants. Carbon dioxide capture and sequestration

(CCS) in deep geologic units is being considered by Florida electric-utilities. Carbon

dioxide-enhanced oil recovery (CO

2

-EOR) is a form of CCS that could offset some of the

costs associated with geologic sequestration. Two potential reservoirs for geologic

sequestration were evaluated in south-central and southern Florida: the Paleocene

Cedar Keys Formation/Upper Cretaceous Lawson Formation (CKLIZ) and the Lower

Cretaceous Sunniland Formation along …

Through The Lens Of A Global Educator: Examining Personal Perceptions Regarding The Construction Of World-Mindedness, Kenneth T. Carano

USF Tampa Graduate Theses and Dissertations

As we embark upon the 21st century, the world is becoming increasingly interconnected. Yet, despite increasing globalization, educational systems are not reflecting this phenomenon. The overwhelming majority of countries, including the United States, still emphasize nationalistic curricula (Parker, 2008; Tye, 2009). Global education is a movement whose supporters advocate an education reflecting the push towards globalization by providing students with the components necessary to live and thrive in an increasingly interconnected world system. Global educators have a common bond as advocates that a global perspective needs to be developed in the classroom. The degree to which this is being done …

Elementary Teachers’ Perceptions Of Technology As A Catalyst For Constructivist Practices In The Classroom: A Case Study, Lynne Brown Menard

USF Tampa Graduate Theses and Dissertations

This research described and analyzed teachers’ perceptions of technology as a catalyst for stimulating classroom constructivist practices. The teachers were located at multiple schools in one Florida county. The teachers were selected based on participation in the Education through Dynamic Global Experiences (EDGE) program. This One-toOne program provides one laptop for every classroom teacher and student. The most frequent ideas in the literature fell into three sections. First is the need to integrate technology as part of the curricula and use constructivism as a theoretical framework for technology integration. The second relates to the best practices of incorporating classroom technology …

Technology Integration For Preservice Science Teacher Educators, Nina C. Stokes

USF Tampa Graduate Theses and Dissertations

The current state of technology integration in science teacher education programs is examined with a view to providing science teacher educators with practical information and diverse examples of technologies they can model in their own courses. Motivators and barriers to technology integration and use are discussed, and recommendations for choosing and evaluating science technologies made. A brief history of how computers, related communication technologies, and science teacher education reform "fit" together is provided. Multiple interpretations of what is meant by "technology" and associated terms (distance learning, online courses, Web-enhanced courses, simulations, authentic data sets etc.) are included to set the …

Antarctic Tunicates And Endophytic Fungi: Chemical Investigation And Synthesis, Matthew D. Lebar

USF Tampa Graduate Theses and Dissertations

Drug discovery is reliant on new developments in natural product chemistry as well as advances in chemical synthesis. The interconnectivity and interdependence of natural and synthetic investigation in drug discovery is evident. The chemical exploration reported herein elaborates the relationship between natural product chemistry and chemical synthesis. Of particular interest are chemicals from organisms residing in less accessible environments, particularly Antarctica and endophytic microbial communities. Degradation via reductive ozonolysis of palmerolide A, a macrocyclic polyketide isolated from the Antarctic tunicate

Synoicum adareanum, and subsequent synthetic preparation of the resulting polyols (1,2,6-hexanetriol and 1,2,3,6-hexanetetraol) led to a revision in the …

Towards Understanding Water Conservation Behavior In Southwest Florida: The Role Of Cultural Models, Bernard T. (Terry) Johnson

USF Tampa Graduate Theses and Dissertations

This applied anthropology dissertation aims to enhance public policy and best practices for conserving potable water resources, using the Tampa Bay region of southwest Florida as a case study. It addresses not how humans conserve, but why they may or may not choose to do so. To date, a limited anthropological focus on water conservation behavior in western, urban settings has created a gap in the role culture plays in understanding why people conserve. The research problem is to identify how water conservation behavior in Tampa, Florida can be enhanced through a better understanding of beliefs and values reflected in …

An Exploration Of Synchronous Communication In An Online Preservice Esol Course: Community Of Inquiry Perspective, Aylin Tekiner Tolu

USF Tampa Graduate Theses and Dissertations

Based on a collaborative and socio-constructivist approach to online education, the Community of Inquiry (CoI) model emphasizes creating an effective learning environment where students feel a connection with other learners and the instructor and engage in well-designed collaborative learning activities.

Following a naturalistic methodology, this qualitative case study investigated the use of synchronous communication for creating a community of inquiry and student satisfaction in an online ESOL (English Speakers of Other Languages) endorsement course for preservice teachers. Elluminate Live was used for class meetings while an instant messenger, Gmail Chat served the needs for impromptu interactions between a student and …

Assessment Of “Community Stepping Stones,” A Community-Based Youth Art Education Program, Jennifer E. A. Pedraza

USF Tampa Graduate Theses and Dissertations

Community Stepping Stones is an art education program whose objective is to

“provide education, mentor children and adolescents, enhance the community

economics, and enrich the quality of life in the community” (Community Steppping

Stones [CSS], 2009a). Community art education programs, particularly for youth, have

become increasingly popular as a way to address and prevent delinquent behavior.

However, art education programs have proven challenging to evaluate and sustain.

The goal of my thesis was to explore how Community Stepping Stones

implemented and evaluated a community-based youth arts education program

compared to other, similar programs and how the organization could make the …

Teacher Attitudes, Perceived Influences, And Self-Reported Classroom Behaviors Related To School Nutrition Environments, Beverly Lawler Girard

USF Tampa Graduate Theses and Dissertations

This study determined attitudes of kindergarten through fifth grade teachers about school nutrition environments, their perceived influence on school nutrition environments, and self-reported classroom behaviors. Specific objectives were to: (a) identify perceived factors that influence the school nutrition environment, according to teachers surveyed; (b) examine relationships between elementary school teacher attitudes about school nutrition environments and perceived influence on the environment; (c) examine relationships between elementary school teachers’ attitudes about school nutrition environments, and self-reported classroom behaviors; (d) examine relationships between perceived influence over the school nutrition environment and self-reported classroom behaviors; and, (e) examine relationships between teachers’ demographic characteristics …

Nautical Knowledge: An Experimental Analysis Of The Influence Of Public Relations Strategies In Safe Boating Communication, Emily N. Guilfoil

USF Tampa Graduate Theses and Dissertations

This study explored the effect of public relations message strategies on beliefs,attitudes, and behavioral intentions of individuals regarding boater safety. An experiment was conducted using seven safety messages. Specifically, Fishbein and Ajzen’s (1975) theory of reasoned action and J.E. Grunig’s (1997) situational theory of publics were used to examine the communication effects of message strategies proposed by Hazleton and Long’s (1988) public relations process model.The findings of this study support the predictions of the theory of reasoned action—that salient beliefs predict attitude toward behavior and attitude toward behavior and subjective norm predict behavioral intent. Of the three attitude items measured—attitude …

An Analysis Of U.S. Policies Targeting The Iranian Nuclear Program, Bryan T. Hamilton

USF Tampa Graduate Theses and Dissertations

Iran’s nuclear program continues to present a major challenge to U.S. policy. At the core of this challenge is one fundamental question: Is Iran attempting to build a nuclear weapon? Objective analysis reveals that Iran’s dependence on oil and natural gas provides sufficient economic merit for Iran to pursue a peaceful nuclear program; without nuclear power to meet rising domestic energy needs, Iran’s economy will suffer. Though the economic justification is valid, the security of Iran and the survival of its regime are overarching; acts of foreign interference in Iran’s affairs have fueled the regime’s quest for a nuclear weapon. …

Evaluation Of Immunogene Therapy Using A Plasmid Encoding Il-15 Delivered By Electroporation In A 3d Tumor Model And A Mouse Melanoma Model, Bernadette Marrero

USF Tampa Graduate Theses and Dissertations

Melanoma is an aggressive disease with few effective treatment options. Non-toxic, anti-tumor therapies and prophylactic approaches are currently being investigated to identify treatment options that will control and remove late-stage melanoma.

The overall goal of this project was to establish an effective delivery method for a plasmid encoding human interleukin (phIL-15) into mouse melanoma cells (B16.F10) using the gene transfer technique electroporation (EP)¹. The EP delivery phIL-15 was optimized using an in vitro 3D tumor model. The purpose was to translate these IL-15 delivery conditions into an in vivo mouse melanoma model to study IL-15 signal transduction and …

Adolf Hitler – America’S First Black President And Other Oval Office Demons: The Right-Wing Rhetorical Assault On Barack Obama’S Health Care Plan, Daniel Ruth

USF Tampa Graduate Theses and Dissertations

This thesis endeavors to examine the imagery and rhetoric surrounding the portrayal of President Barack Obama during the national debate over health care reform from the summer of 2009 into the spring of 2010. It is argued that the critics of the health care reform legislation used images to portray the president as Adolf Hitler, Che Guevara, The Joker, as well as other images such as the swastika and the Wehrmacht symbol as stand-in euphemisms for race to discredit Barack Obama.

A number of exemplar images have been selected from various websites and publications specifically addressing the portrayal of Barack …

Microbial Influences On Karst Dissolution: The Geochemical Perspective, With A Chapter On Assessment Of The Spreadsheets Across The Curriculum Project, Dorien Kymberly Mcgee

USF Tampa Graduate Theses and Dissertations

Microbes are prevalent in geologic settings and a growing body of research suggests the roles they play in geologic processes may be more important than previously thought, and therefore underestimated. This dissertation addresses the influence of microbes on the dissolution of limestone in karst settings by analyzing the stable carbon isotopes and geochemistry of air and waters from three unique cave and karst settings: West-Central Florida, the Everglades (southern Florida) and The Bahamas. In Florida, these parameters as well as air/water temperature, rainfall, and water-level fluctuations were monitored for 22 and 10 months. In the Bahamas, geochemical data were collected …

Contextualizing Obesity Among Latino Farmworkers: A Critical Analysis Of Structural And Cultural Processes Affecting Farmworker Health And Nutrition In Central Florida, Melissa H. Johnson

USF Tampa Graduate Theses and Dissertations

Migrant and seasonal farmworkers play a critical role in the U.S. economy, producing food for the American public, while their suffering is often rendered invisible by their existence on the margins of society. The low wages associated with farm labor combined with the largely undocumented status of this population severely limits access to food, housing, and health care, resulting in poor health outcomes. Through the use of a critical anthropological approach, this research examines the social, cultural, political, and economic context of obesity among Latino migrant and seasonal farmworkers in Central Florida. Ethnographic research methods were used to explore perceptions …

More Than Bows And Arrows: Subversion And Double-Consciousness In Native American Storytelling, Anastacia M. Schulhoff

USF Tampa Graduate Theses and Dissertations

W. E. B. Du Bois‘ legendary reflections on the ―peculiar sensation, this double-consciousness, this sense of always looking at one‘s self through the eyes of others‖ has been applied almost exclusively to the souls of African American people (Du Bois 1903). This thesis shows how the concept of double-consciousness is alive in the stories told by Native Americans. I draw upon data from two websites that have recorded the stories told by ―exemplary indigenous elders, historians, storytellers and song carriers‖ and their oral traditions that serve the ―purpose of cultural preservation, education, and race reconciliation‖ (Wisdom of the Elders, 2009). …

Comparison Of Risky Decision Making Processes In Dyads And Individuals, Moumita Mukherjee

USF Tampa Graduate Theses and Dissertations

The thesis compared the likelihood of taking risks in dyads and individuals in

varying situations. Patterns of risky decision making were examined in the standard risky

choice task and a novel risk management task. The relative successes of two theories of

risky decision making were assessed: Prospect Theory emphasizes perceptual and

psychophysical processes, whereas Security-Potential/Aspiration Level Theory

emphasizes dispositional and motivational processes. The thesis also examined dyads’

decision behavior in light of competing social influence perspectives regarding risky

versus cautious shifts and group polarization.

Participants, as individuals or as part of a dyad, made decisions in 23 trials about

hypothetical …

Plasma Mediated Molecular Delivery, Richard J. Connolly

USF Tampa Graduate Theses and Dissertations

Non-viral delivery of plasmid DNA has traditionally relied upon physical forces applied directly to target tissues. These physical methods typically involve contact between an applicator and the target tissue and often cause transient patient discomfort. To overcome the contact-dependent limitations of such delivery methodologies, an atmospheric direct current plasma source was developed to deposit ionized gas molecules onto localized treatment sites. The deposition of charged species onto a treatment site can lead to the establishment of an electric field with strengths similar to those used for traditional electroporation. In vitro experiments proved that this technology could transiently permeabilize cell membranes …