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Procedures For Assessment Of The Marine Carbon System And Evaluation Of Access And Ocean Acidification Literacy In The Deaf Community, Loraine Martell-Bonet

USF Tampa Graduate Theses and Dissertations

One major goal of this dissertation addresses the need for improved laboratory procedures to measure chemical changes in seawater caused by the uptake of atmospheric CO2, especially in coastal environments. Due to the proximity of anthropogenic stressors in the coastal zone, the biogeochemical complexity of the CO2 system is more dynamic than the open ocean. My principal research objectives were addressed by developing methods for (1) improving the accuracy of pH measurements over a wide range of salinities and (2) investigating the acid-base properties of natural organics in the coastal ocean.

The accuracy of pH electrode calibrations is affected when …

Deep Learning-Based Automatic Stereology For High- And Low-Magnification Images, Hunter Morera

USF Tampa Graduate Theses and Dissertations

Quantification of the true number of stained cells in specific brain regions is an important metric in many fields of biomedical research involving cell degeneration, cytotoxicology, cellular inflammation, and drug development for a wide range of neurological disorders and mental illnesses. Unbiased stereology is the current state-of-the-art method for collecting the cell count data from tissue sections. These studies require trained experts to manually focus through a z-stack of microscopy images and count (click) on a hundred or more cells per case, making this approach time consuming (~1 hour per case) and prone to human error (i.e., inter-rater variability). Thus, …

Developing New Strategy Toward Ruthenium And Gold Redox Catalysis, Chenhuan Wang

USF Tampa Graduate Theses and Dissertations

This dissertation mainly contains three parts: 1) The discovery of triazole-modified Ru carbene catalysts for alkene metathesis and dynamic covalent chemistry; 2) The study of ligand assisted gold oxidative addition toward aryl iodide to achieve the alkene difunctionalization; 3) Gold redox catalysis via chiral P,N-chelating ligands. In the first part, the 1,2,3-triazole coordinated ruthenium carbene complexes (TA-Ru) were reported for the first time as a new class of modified Grubbs catalyst to achieve challenging olefin metathesis at higher temperatures without catalyst decomposition. With this new TA-Ru catalyst, cross-metathesis (CM), ring-closing metathesis (RCM) and dynamic covalent chemistry (DCvC) were achieved. The …

Booker Creek: Wet Season Water Quality Trends In A Florida Urban Stream, Alexandra M. Shostak

USF Tampa Graduate Theses and Dissertations

Booker Creek, a 1.9-mile stream with a 4.9-mi2 watershed, flows southeast through highly urbanized St. Petersburg, Florida, and empties into Bayboro Harbor, which connects with Tampa Bay. This study collected and analyzed water quality data for several parameters (TN, TP, nitrate-nitrite, ammonia, orthophosphate, chl-a, fecal coliform, dissolved oxygen, pH, turbidity, temperature, and salinity) every two weeks, at four different sites, during the 2022 wet season to characterize the current state and geographic variability of Booker Creek. Current creek sampling regimes, though consistent, do not sample at multiple sites along the creek. Urban streams do not display uniform water quality along …

Water Chemistry Variability In Peacock Springs Cave System And Spring Basin: Impacts On Continuous Monitoring Of Karst Aquifers, Mahnoor Kamal

USF Tampa Graduate Theses and Dissertations

The Upper Floridan Aquifer (UFA) is a highly transmissive and heterogeneous karst aquifer that makes it acutely susceptible to pollution. Rapid and extensive water quality changes in nutrients at springs draining the UFA have prompted intensive monitoring at several priority springs, including continuous and discrete water quality sampling. Springs, however, are a discrete discharge point of an often-expansive phreatic cave network that can intersect different land uses and thus drain distinct geographic areas of a springshed. The extensive network contains convergent passages from deep and shallow aquifer regions and may contribute to varying water chemistry across passages resulting in water …

Affordances And Limitations Of Molecular Representations In General And Organic Chemistry, Ayesha Farheen

USF Tampa Graduate Theses and Dissertations

Molecular representations that show chemical bonding are ubiquitous in general and organic chemistry. These help in communicating chemistry concepts that are fundamental to understanding how and why molecules interact. The goal of this dissertation work is to offer an evidence-based report on the affordances and limitations of the variety of molecular representations by chemistry topic. The variety of representations investigated are chemical formula, Lewis dot structure, line-angle diagram, ball and stick, space filling, and electrostatic potential maps. Five studies were conducted with general and organic chemistry students and three key findings emerged. First, affordances and limitations of molecular representations are …

Time To Check In: The Effectiveness Of Modified Check, Connect And Expect For Children Of Incarcerated Parents, Talia E. Shuman

USF Tampa Graduate Theses and Dissertations

Parental incarceration affects approximately 3.5% of minors in the United States, or about 1 in every 28 children (Federal Interagency Forum on Children and Family Statistics, 2015). Parental incarceration is considered an adverse childhood experience (ACE). ACEs like parental incarceration are positively correlated with increased risk of injury, health problems, poorer attention and decision-making abilities, and difficulties forming healthy relationships (Center for Disease Control and Prevention, 2020). In addition, children who have experienced parental incarceration are also more likely to show increased internalizing and externalizing behaviors when compared to same-aged peers who do not have an incarcerated parent (Shlafer & …

Labile Dissolved Nickel (Ni) Concentrations In The North Pacific, Calyn M. Crawford

USF Tampa Graduate Theses and Dissertations

Nickel (Ni) is an important micronutrient for phytoplankton and bacteria that serves as a required co-factor in several metalloenzymes. Despite these known biological uses, total dissolved Ni concentrations remain elevated in global surface waters, in contrast to the surface depletion commonly observed for macronutrients and other nutrient-type trace elements. A prevailing hypothesis for the muted depletion of dissolved Ni concentrations in surface waters is that dissolved Ni in seawater is not in a bioavailable form. The chemical lability of Ni in seawater provides insight into Ni speciation and bioavailability, but few measurements have been made in the open ocean to …

Time-Series Analysis Of Coral Reef And Reef-Fish Biodiversity In The Florida Keys National Marine Sanctuary, Cara L. Estes

USF Tampa Graduate Theses and Dissertations

The general decline in coral-reef ecosystem health throughout the Caribbean between the 1970’s and late 1990’s seems to have slowed down or stopped, with only slight decreases in coral reef cover observed in the 2000’s through 2019. The earlier decline can be attributed to many different local and regional stressors that have acted together, and include naturally occurring (hurricanes, diseases) and anthropogenic pressures (increased sedimentation, fishing). These ecosystems provide a range of services to people, including food and protection from storms, with an annual benefit of billions of dollars. This has driven a desire to better understand these ecosystems. The …

Understanding Controls On Spring Hydrographs In An Eogenetic Karst Aquifer In North-Central Florida, Ryan Almeqhem

USF Tampa Graduate Theses and Dissertations

Florida has one of the largest karst aquifers in the United States, with a significant portion of its water resources originating from the eogenetic karst system in north central Florida. Despite the importance of this system, eogenetic karst still needs to be studied compared to telogenetic karst. The hydrological behavior of karst aquifers is complex, with numerous factors influencing the timing, magnitude, and shape of spring hydrographs. One hypothesis, by Florea and Vacher (2006), suggests that matrix permeability is the primary control of spring hydrographs in karst systems. However, this thesis challenges this hypothesis by presenting evidence that the recharge …

Improving Interview Skills Through Virtual Reality With Behavior Skills Training For Students With Disabilities, Garrit Dubois

USF Tampa Graduate Theses and Dissertations

Individuals with disabilities experience lower employment rates compared to the general population, highlighting the need for interventions to enhance their employability. Interview skills are the most crucial skills to develop. Researchers have used behavioral skills training (BST) to improve vocal and non-vocal aspects of interviewee behavior. Additionally, virtual reality (VR) has been used to simulate the interview process and promote generalization of skills across various environments. However, limited research has investigated the use of VR for vocational skills training in students with disabilities, particularly those still enrolled in high school. This study aimed to examine the use of VR combined …

Mining Multibeam Transit Data For Deep Ocean Polymetallic Nodules: Case Study In Se Pacific Ocean, Mark E. Mussett

USF Tampa Graduate Theses and Dissertations

Occurrences of nodules on the seafloor represent benthic habitats and present potential deposits of economically important metals. Better understanding their extent throughout Earth’s oceans will aid in resource management and determining different factors which influence their formative factors and respective local characteristics. Multibeam bathymetry and backscatter data, along with auxiliary data suggest an area of seafloor near the Foundation Seamount chain in the southern Pacific Ocean, centered around -36.84° latitude, -119.75° longitude, contains a previously uncharted polymetallic nodule occurrence. Our analysis of Simrad EM12 DUAL (12 kHz) multibeam data, collected during a 1997 transit, shows an area of strong backscatter …

Discovery Of Small-Molecule Inhibitors For The Β-Catenin/B-Cell Lymphoma 9 Protein-Protein Interaction., Zilu Li

USF Tampa Graduate Theses and Dissertations

The Wnt/β-catenin signaling pathway is associated with activating genes that support cell proliferation, differentiation, and survival. Excessive activation of Wnt/β‐catenin signaling has been associated with development of several types of cancer and activation of stem-like cancer cells. Direct targeting of β‐catenin, the central hub in this signaling pathway, is a promising strategy to suppress the hyperactive β‐catenin signaling but has proven to be highlychallenging. Substantial efforts have been made to discover compounds that bind with β‐catenin, block β‐catenin mediated protein–protein interactions (PPIs) and suppress β‐catenin signaling. β‐catenin/BCL9 PPI has a smaller contacting area (1450 Å2) and a moderate binding affinity …

Knowledge Integration In Domain-Informed Machine Learning And Multi-Scale Modeling Of Nonlinear Dynamics In Complex Systems, Phat K. Huynh

USF Tampa Graduate Theses and Dissertations

Nonlinear dynamical systems have been extensively used to model various phenomena in the changing world around us, especially in science and engineering fields. Thanks to breakthrough advancements in sensing technologies, an increasingly high volume of multi-modal sensor data has been collected, which enables us gain better insights into complex systems dynamics and build sophisticated data-driven machine-learning-based dynamic models without having the access to the underlying governing equations. However, integrating domain-specific knowledge in machine learning algorithms remains pivotal for various reasons: it promises enhanced predictive accuracy, better model interpretability, and increased generalizability. This dissertation delves into three core research questions, each …

Predictors Of Growth In Socioemotional Functioning From Kindergarten Entry To 5th Grade For Students With And Without Disabilities, Sydney Shupe

USF Tampa Graduate Theses and Dissertations

The link between socioemotional functioning and academic achievement is well established, as is the evidence showing that children with disabilities often develop socioemotional functioning differently than peers without disabilities. However, few studies have considered how early skills are associated with growth of socioemotional functioning for children, and how that may differ for children with disabilities. Therefore, the purpose of this study was to examine the effect of academic, social, and behavioral skills at the start of kindergarten on the growth of socioemotional functioning from kindergarten to 5th grade; early socioemotional functioning, academic and cognitive skills were analyzed in a series …

Tree Planting As An Adaptive Management Tool For Climate Change In Morocco, Stephanie J. Robinson

USF Tampa Graduate Theses and Dissertations

The practice of planting fruit trees to combat climate change impacts in Morocco has been growing in popularity with support from the Moroccan government and non-profit organizations. Although it is often considered a successful adaptive management tool, tree planting initiatives (TPIs) in arid lands have not been thoroughly analyzed, their management is unstandardized, and these initiatives can cause harm when trees are planted in areas that cannot support them. To implement tree planting better these TPIs need to find areas where the trees they are introducing have the best chance of survival without negatively impacting the surrounding ecosystem and human …

Evaluating Methods For Improving Dnn Robustness Against Adversarial Attacks, Laureano Griffin

USF Tampa Graduate Theses and Dissertations

Deep learning has become more widespread as advances in the field continue. As aresult, making sure deep learning is safe has become a priority. A seemingly normal image with intentional pixel changes can cause a well-trained model to misclassify the image with high confidence. Those kinds of images are called adversarial attacks. Adversarial training has been developed to defend against adversarial attacks. This thesis evaluates different adversarial training methods against a variety of adversarial attacks. The key metrics for evaluation are classification accuracy and training time. This thesis also experiments with an improvement on an existing adversarial training method, the …

Chemical Analysis Of Metabolites From Mangrove Endophytic Fungus, Sefat E Munjerin

USF Tampa Graduate Theses and Dissertations

Natural products hold a significant place in drug discovery for their abundant and unique secondary metabolites. These secondary metabolites which encompass a range of organic compounds such as alkaloids, phenols, peptides, flavonoids, polyketides, and terpenoids, can easily be exploited for drug development. Endophytic fungi, residing harmoniously with its host, have emerged as generous producers of bioactive secondary metabolites, displaying efficacy against a wide array of human pathogens, including the challenging ESKAPE pathogens.

The research explores endophytic fungi and their prospective for drug discovery and development through the synthesis of distinctive secondary metabolites. Endophytic fungus, HM13-26C-2B, collected from Honeymoon Island, Florida …

Effects Of Downdrag On Pile Performance, Ruthvik Pendyala

USF Tampa Graduate Theses and Dissertations

The behavior of piles under varying load conditions has sparked significant interest and research within the geotechnical engineering field. Specifically, in the context of MSE wall-type embankments, piles are installed before embankment construction commences. In these scenarios, soil settlement beneath the embankment initiates following pile installation, consequently generating downward lateral shear loads on the piles, known as downdrag.

Research pertaining to the downdrag phenomenon in cohesionless granular soils is limited. Hence, the primary focus of this study revolves around examining the impact of downdrag on pile performance in sandy soils using numerical modeling as the investigative approach. To accomplish this, …

The Development Of A General Watershed - Highway Mathematical Model, Robert W. Higgins

USF Tampa Graduate Theses and Dissertations

No abstract provided.

Use Of Silica Dust And Lunar Simulants For Assessing Lunar Regolith Exposure, Layzamarie Irizarry-Colon

USF Tampa Graduate Theses and Dissertations

The utilization of the lunar regolith holds immense potential for future space exploration, but the potential health risks associated with lunar regolith are a significant concern. This literature review seeks to critically assess the use of silica and lunar simulants standards and permissible exposure limits (PELs) to evaluate the adverse health effects of lunar regolith. The literature review covers an extensive analysis of existing research, health risks, toxicological properties, and physical and chemical composition associated with silica, lunar simulants, and lunar regolith by using three main sources: regulations, scholarly articles, and Kennedy Space Center (KSC) discussion. This comprehensive analysis forms …

Stable Isotope Analysis On Yellowfin And Blackfin Tuna Eye Lenses Reveals Life History Patterns In The Gulf Of Mexico, Kylee M. Rullo

USF Tampa Graduate Theses and Dissertations

Spatial geography, ontogenetic movement, and trophic patterns of mobile species are key elements of effective marine resource management. A number of methods are currently available for tracking movements of pelagic migratory species, including the use of conventional and electronic tags. However, tagging campaigns can take years to provide useful data, can be expensive, and only capture a portion of a fish’s lifetime. For this project, I used stable-isotopic ratios of nitrogen (δ15N) and carbon (δ13C) in metabolically inert, chronologically-layered fish-eye lenses to explore lifetime movement and trophic patterns of yellowfin tuna (Thunnus albacares) and blackfin tuna (Thunnus atlanticus) in the …

A Psychometric Analysis Of Natural Language Inference Using Transformer Language Models, Antonio Laverghetta Jr.

USF Tampa Graduate Theses and Dissertations

Large language models (LLMs) are poised to transform both academia and industry. But the excitement around these generative AIs has also been met with concern for the true extent of their capabilities. This dissertation helps to address these questions by examining the capabilities of LLMs using the tools of psychometrics. We focus on analyzing the capabilities of LLMs on the task of natural language inference (NLI), a foundational benchmark often used to evaluate new models. We demonstrate that LLMs can reliably predict the psychometric properties of NLI items were those items administered to humans. Through a series of experiments, we …

Entanglements Of Teenage Food Security Within High School Pantries In Pinellas County, Florida, Karen T. Díaz Serrano

USF Tampa Graduate Theses and Dissertations

Food insecurity has the ability to shape an individual’s or a family’s everyday life and take emotional, psychological, and physical tolls. Among adolescents, not having access to nutritious food could lead to physical effects during growth and development. Moreover, the stress and anxiety of not knowing where their next meal is coming from and the social stigma associated with being food insecure can have negative effects on mental health. A recent solution to these issues is locating food pantries in high schools. Purpose: This exploratory study examined how high school students in Pinellas County, Florida perceive the use of a …

Storying The Experiences Of A First-Year Teacher’S Mathematics-Related Teacher Identity With A Communities Of Practice Lens, Kelly Jean Navas

USF Tampa Graduate Theses and Dissertations

This longitudinal narrative inquiry explores the influences on a first-year elementary teacher’s mathematics-related teacher identity, employing a community of practice lens. Identity is a complex concept. In this study, identity is viewed as socially constructed through new experiences. These social interactions can be seen as an individual participates in their communities of practice. A mathematics-related teacher identity intertwines a teacher’s mathematical identity with their teacher identity. This can influence their classroom decisions, as well as their planning and implementation of mathematics lessons. To gain insights into the evolution of this mathematics-related teacher identity, I listened to the stories of Suzie, …

Thermodynamic Frustration Of Tad2 And Prr Contribute To Autoinhibition Of P53, Emily Gregory

USF Tampa Graduate Theses and Dissertations

The intrinsically disordered transcription factor and tumor suppressor p53 binds to promoter response element DNA upon cellular stress and activates genes associated with cell cycle arrest, senescence, and apoptosis. Disruption of sequence specific binding to target gene promoters is heavily implicated in human health, where a majority of cancers contain mutations localized to the DNA binding domain (DBD) of p53. p53 DNA binding is regulated by posttranslational modifications, associations with cellular factors, and by an autoinhibitory intramolecular interaction. The autoinhibitory intramolecular interaction occurs when the disordered N-terminal transactivation domain (TAD) interacts with the ordered DBD. Previous work in the Daughdrill …

Cyber-Physical Multi-Robot Systems In A Smart Factory: A Networked Ai Agents Approach, Zixiang Nie

USF Tampa Graduate Theses and Dissertations

This dissertation focuses on addressing the technical challenges of non-stationarity in smart factories through the use of cyber-physical AI agents. Industry 4.0 and smart manufacturing with smart factories as a central role, have a growing demand for Just-in-Time (JIT) and on-demand production, as well as mass customization—all while maintaining high productivity, resource efficiency and resilience. This research positions Multi-Robot Systems (MRS)-driven smart factories. The heterogeneous production and transportation robots in an MRS collaborate to form multiple real-time adjusted production flows achieving the flexibility to accommodate such on-demand, mass customization.

However, the implementation of MRS introduces new sets of challenges, including …

The Process Of Government In Clearwater, Florida, Picot Deboisfeuillet Floyd

USF Tampa Graduate Theses and Dissertations

Based upon participant observation as city manager, the dissertation examines political processes involved in four episodes relating to the government of the City of Clearwater, Florida: the selection of a site for a branch campus of the University of South Florida, the application of technology assessment techniques to growth issues, attempts to regulate a pornographic movie and supply store, and the arrest of the city attorney. Non-mathematical game theory is used as a model for the analysis, which demonstrates that game-like processual activity takes place in a governmental structure based upon cooperation. Such analysis can have a direct impact on …

Development Of Bioactive Peptidomimetics, Lei Wang

USF Tampa Graduate Theses and Dissertations

In the past two decades, a lot of different peptidomimetics, such as β-peptides, oligoureas, azapeptides, peptoids, aromatic oligoamides, and others, have been developed. These peptidomimetics with distinctive scaffolds have shown potential in protein surface recognition and mimicry, PPI regulation, catalysis, etc. Moreover, these bioactive peptidomimetics demonstrate much better bioavailability and biostability than the bioactive peptides. To expand the application of peptidomimetics, our group have developed a new sequence-specific peptidomimetics- γ-AApeptide, which is derived from chiral γ-PNA. It shows excellent advantages including highly resistant to various proteolytical degradations and has the potential to increase chemodiversity. γ-AApeptide also exhibits remarkable biomedical applications, …

Development Of Antiviral Peptidomimetics, Songyi Xue

USF Tampa Graduate Theses and Dissertations

Protein-protein interactions (PPIs) are essential for biological processes and are associated with a number of diseases, including cancer, infectious diseases, and neurodegenerative diseases. As a result, modulation of PPIs has been recognized as one of the most promising strategies to develop the novel drugs. Peptide modulators always exhibit higher specificity and affinities with targets than small compounds or monoclonal antibodies, but their broad medicinal effectiveness is constrained by their poor bioavailability and biostability. Peptidomimetics, which have been developed to mimic the structure as well as function of bioactive peptides and proteins, have shown excellent potential in protein surface mimicry and …