Digital Commons Network^™

“Urban Renewal” Surrounding Florida Solar Plants: A Study On Low-Carbon Gentrification, Megan Amber Grove

USF Tampa Graduate Theses and Dissertations

Renewable energy and sustainable living are growing trends in Florida as the state “develops” rural landscapes and “redevelops” urban ones, resulting in neighborhood changes, including displacement of low-income and minority populations (LIMP). Solar energy has not historically been a root cause of such changes, but in recent years it has increasingly accompanied neighborhood development which prioritizes the interests of the elite and middle class. As such, it has become a contributor to “urban renewal,” which must be addressed to avoid future LIMP displacement. This study used geospatial and data science technologies and techniques to empirically explore and analyze urbanization and …

A Differentially Methylated Region Analysis Between Three Disease States Of Major Depressive Disorder In Primarily African-American Cohorts, Lanie Katelynn Mullins

USF Tampa Graduate Theses and Dissertations

Major depressive disorder (MDD) is a common and debilitating disorder that affects millions of people worldwide. MDD is a multifactorial disease with no established mechanism currently able to explain all facets of the disease or its etiology. Feedback loop mechanisms have been posited to explain the interactions of psychological and physical components of the disease etiology. Epigenetics, specifically DNA-methylation analyses, can shed light on the interaction between the within-person environment resulting from MDD symptomology, the persistence of the disease, and factors influencing remission. Here, a differentially methylated region analysis was conducted using samples derived from whole blood to interrogate the …

Single And Dual Cell Encapsulation Via Liquid Core Hydrogels Using Pico Injection, Jade Houston

USF Tampa Graduate Theses and Dissertations

In this project, a comprehensive microfluidic device, along with several interdependent subsystems, was designed and developed in SolidWorks, leveraging a combination of 3D resin printing with biocompatible resin and micromachining with polymethylmethacrylate (PMMA). The microfluidics chip integrated both passive and active components to facilitate its primary function: dual cell encapsulation via liquid core sodium alginate hydrogel droplets.

Integral to the microfluidics chip design were key features including a 5-convolution Archimedes spiral for particle sorting, a flow focusing junction for droplet generation, and a pico injector for injecting additional cells into passing droplets via the manipulation of an electric field.

An …

Development Of A Plant Growth And Health Monitoring System Using Imaging And Sensor Array Information For Cubesat Applications, Kat-Kim Phan

USF Tampa Graduate Theses and Dissertations

Space exploration has been a topic of interest in the scientific community, such as the planned missions to Mars. To accomplish this would require being able to provide astronauts with a steady supply of food beyond freeze-dried foods leading to the need to grow food in space. Although this is a topic still being investigated, the CubeSat platform opens the possibility of carrying out studies on plant growth under more strenuous space conditions unlike that in the International Space Station (ISS). Developing a plant-focused mission for a CubeSat, though, entails being able to develop a system to sustain plant life …

On The Possibility Of Secular Morality, Zachary R. Alonso

USF Tampa Graduate Theses and Dissertations

Is something like a transcendent God necessary to have a coherent moral system, or at the very least, necessary for one to have personal ethical guidelines to live by that are not entirely subjective? This paper explores the claims of two Christian thinkers, David Bentley Hart and D. C. Schindler, as well as some alternatives to their thinking in the ethics of Alasdair MacIntyre and Jeffrey Stout. Hart and Schindler portray society as downcast, riddled with moral decay, and the only solution to this decay is to turn toward God, thereby recognizing a hierarchical, cosmological worldview. While MacIntyre believes that …

Preliminary Investigation Of Differences In Host Mitochondria Membrane Potential During Toxoplasma Gondii Infection, Bryan L. Ortega

USF Tampa Graduate Theses and Dissertations

Toxoplasma gondii is an obligate intracellular protozoan with a broad host range of hosts, including various warm-blooded mammals, including humans. While infections can often be asymptomatic in immunocompetent individuals, they can pose significant risks to those who are immunocompromised, as well as pregnant women. Toxoplasmosis represents a significant burden on public health in the United States, with over 800,000 annual infections reported, often leading to severe complications such as symptomatic eye disease and congenital infections. Recent attention has developed regarding its potential association with human neuropathies such as Alzheimer's disease, dementia, and mood disorders. This project investigated differences in host …

Educator And Caregiver Perceptions Of Project Begin Well: An Early Childhood Risk Identification Course, Samin Khallaghi

USF Tampa Graduate Theses and Dissertations

The early years of a child's life are vital to building the foundation for early childhood, and educators and caregivers are at the heart of making this foundation for children. However, educators and caregivers often lack knowledge on correctly identifying risks in children. The current study used a quantitative non-experimental design to examine early childhood educator and caregiver perceptions of a training program called Project Begin Well. Project Begin Well included a risk identification course for educators and caregivers of children aged 3-5 years. Specifically, educators' and caregivers' knowledge, skills, attitudes, aspirations, and behaviors toward the risk identification course were …

Dissolved Nickel Speciation At The Bermuda Atlantic Time-Series Study (Bats) Site, Caitlyn E. Parente

USF Tampa Graduate Theses and Dissertations

Here I present the first depth profiles of dissolved nickel (DNi) speciation over an annual cycle at the Bermuda Atlantic Time-series Study (BATS) site in the North Atlantic. Seawater samples were collected in March, May, August, and November 2019 and analyzed for labile DNi using competitive ligand exchange-adsorptive cathodic stripping voltammetry (CLE-AdCSV). Labile DNi was measured by CLE-AdCSV as the fraction of DNi that exchanges with an added competitive ligand in an overnight equilibration. Results revealed two distinct fractions of DNi present throughout the water column: a labile fraction, and a strongly organically complexed (i.e., non-labile) fraction. More than 50% …

The Perception Of Innocence And Guilt: Understanding Relationships Between Exonerees And Their Loved Ones, Martha E. Mccullough

USF Tampa Graduate Theses and Dissertations

Exoneration and wrongful conviction complicate the field of criminology and the criminal justice system. Much of the scholarship on wrongful conviction focuses on the faults of the criminal justice system, such as false or misleading forensic evidence, false confessions, official misconduct, inadequate legal defense, and more. However, there is less research on the social, psychological, and economic impact of a wrongful conviction on those who have been wrongfully convicted and those associated with wrongful convictions (e.g., exoneree's loved ones). Recent studies indicate that social and psychological implications after a wrongful conviction may not only affect exonerees but also their loved …

An Analysis Of Therapeutic Alliance And Group Cohesion In A Group Positive Psychology Intervention Serving Middle School Students Reporting Low Life Satisfaction, Alexis Taylor

USF Tampa Graduate Theses and Dissertations

While previous research has focused on how positive psychology treatments relate to outcomes, it is also essential to evaluate the therapeutic process of these treatments. The therapeutic process consists of elements like the therapeutic alliance and the efforts the clinicians and the client put into the process (Bordin, 1979). Positive psychology intervention therapeutic processes differ from traditional treatments, as they have a skills-building perspective rather than a problem-focused perspective (Cunha et al., 2019; Datu & Bernardo, 2020; Kwok et al., 2016). Thus, the therapeutic relationship may take on a different form. Furthermore, as therapy approaches differ, so do the modalities …

The Effect Of The Covid-19 Pandemic On The Environmental Perceptions Of College Students, Sydney H. Kaylor

USF Tampa Graduate Theses and Dissertations

In this study, the environmental perception changes in college students during the COVID-19 pandemic were investigated. During the almost worldwide lockdown between March and December 2020, many students experienced dramatic changes in their socioeconomic life that have affected their well-being, ability to learn, and environmental perceptions. Students were sent home from college campuses, many businesses were shut down, and changes were seen in the environment throughout the world. The many industries and businesses that were shut down decreased the pollution that was in the atmosphere, in our water, and on the land. In many places, lockdown policies lead to improved …

Metatranscriptomic Insights Into The Interaction Between Microcystis Blooms And Hydrogen Peroxide, Taylor L. Hancock

USF Tampa Graduate Theses and Dissertations

For this dissertation I studied the interactions of hydrogen peroxide and cyanobacterial harmful algal blooms (cHABs) dominated by Microcystis in the Caloosahatchee River, FL. Specifically, I examined how taxonomic and functional succession relates to both natural hydrogen peroxide dynamics and hydrogen peroxide added to the environment as a cHAB treatment. Chapter 2 investigated how taxonomic succession and gene expression patterns relate to blooms and associated elevated hydrogen peroxide concentrations. The results showed that gene expressions related to cyanobacterial growth were positively correlated with hydrogen peroxide. The genes identified included enzymes capable of hydrogen peroxide generation which were expressed by blooming …

Mentorship In A Virtual World: How Remote Work Relates To Mentoring Relationships And Outcomes, Alyssa M. Lezcano

USF Tampa Graduate Theses and Dissertations

In the context of an increasingly virtual workplace, it is critical to understand how remote and hybrid work arrangements relate to key developmental, interpersonal relationships at work, such as mentoring relationships. Guided by the existing mentoring literature as well as leader distance theory, the present study aimed to examine the mentorship experiences of early-career protégés who worked remote and hybrid hours. Three waves of data were collected to investigate the influence of remote work and mentor distance on protégés’ perceptions of mentoring support received. Specifically, the study examined protégés’ degree of remote work, perceptions of social and spatial distance between …

Factors Impacting Genetic Test Result Recall And Adherence To Cancer Risk Management Recommendations Among Patients With Germline Pathogenic Variants, Karishma Prakash Bharwani

USF Tampa Graduate Theses and Dissertations

Only 20-60% of information from medical appointments is estimated to be retained of which about half is recalled accurately; however there remains limited information about recall following disclosure of inherited cancer genetic test results (GTR). We sought to evaluate recall of GTRs and the association of recall with adherence to recommended cancer risk management (CRM). Study participants were consented individuals with a confirmed germline pathogenic or likely pathogenic variant (GPV) in an inherited cancer predisposing genes for which there are CRM guidelines per the National Comprehensive Cancer Network (NCCN). Through information collected through self-reported surveys, adherence to CRM was defined …

From The Patient’S Perspective: Understanding The Colorectal Cancer Patient Experience Portrayed In Edutainment Television, Allison M. Fisher

USF Tampa Graduate Theses and Dissertations

This study qualitatively examined how edutainment television portrayed the colorectal cancer (CRC) patient experience, and whether it reflects the real-life CRC patient experience. This was done by first facilitating a textual analysis of edutainment television episodes from popular medical dramas and the docuseries Country Ever After. Interviews with real-life CRC patients were then facilitated to gather perspectives on whether edutainment television’s depictions were reflective of their experiences. This study utilized cultivation theory to identify whether edutainment television reinforces stigmas about the CRC patient experience. Additionally, uses and gratifications theory was implemented to investigate how CRC patients interact with media platforms …

Temporospatial Deep Learning Strategies For Prediction Of Disease Progression In Radiology, John D. Mayfield

USF Tampa Graduate Theses and Dissertations

While the fields of machine learning and medicine are deeply rooted in axiomatic scientific principles, there is an element of art that makes the practice imperfect, yet innately human. As the two fields have seen the greatest overlap in their collective history, there remains a chasm between them in terms of practical translation for the patients who desire and deserve personalized medicine. As presented in this dissertation, I and my collaborators have contributed to the groundwork for future exploration of predicting disease progression by identifying signals within sequential medical imaging to provide a temporospatial relationship upon which we can make …

The Cultural Relevance Of The Well-Being Promotion Program, Gabrielle Francis

USF Tampa Graduate Theses and Dissertations

Schools are well-situated to be a primary setting to provide mental health services to youth. In fact, most minoritized students who receive mental health services do so through school (Ali et al., 2019). However, even school-based mental health services are not equitably accessed and provided for diverse populations of students, and minoritized students are less likely to have their mental health needs met in schools (Gudiño et al., 2009; Thomas et al., 2011). There are several reasons for the disparity in mental health services amongst students including the cultural relevance of interventions (Santiago-Rosario et al., 2021) and the early departure …

Genetic Counseling Student Self-Efficacy, Exam Anxiety, And Opinions Related To Study Materials For The Genetic Counseling Board Certification Exam., Jessica F. Stanton

USF Tampa Graduate Theses and Dissertations

The Genetic Counseling Board Examination is a critical assessment for clinical practice in the field. However, the relationship between students’ test-related anxiety, confidence in their ability to study (i.e., self-efficacy), and the use of gamification for board examination preparation remains unexplored. This study compared gamification and traditional quiz-style study methods using two randomized groups of students preparing for the Genetic Counseling Board Examination. The primary objectives were to assess for changes in exam-related anxiety and self-efficacy after completing the respective study materials and to evaluate the acceptability and completion rates of these materials. Ninety-eight genetic counseling students or recent graduates …

Formulating Utopian Pasts: Time As A Coping Method For Lgbtq+ Floridians, Elise M. Kuechle

USF Tampa Graduate Theses and Dissertations

Florida has emerged in recent years as a breeding ground for a new generation of anti-LGBTQ+ legislation. The state has introduced 10 anti-LGBTQ+ laws so far in 2023, touching on issues such as bathroom access, healthcare, and education. This paper analyzes the experiences of queer and transgender people living in Florida to ask: How do LGBTQ+ people in Florida cope phenomenologically with rising anti-LGBTQ+ discrimination? Drawing on data from 22 interviews with LGBTQ+ people situated in and around Florida in 2023, this paper captures a distinct political moment for the people that I spoke with, as anti-LGBTQ+ sentiments continue to …

Androgen Drives Melanoma Invasiveness And Metastatic Spread By Inducing Tumorigenic Fucosylation, Qian Liu

USF Tampa Graduate Theses and Dissertations

Melanoma incidence and mortality rates are historically higher for men than women, with an estimated ~47% more new cases and twice the lethality in men in the US in 2023. Consistent with these discrepancies, emerging studies have highlighted the tumorigenic role of the male sex hormone androgen and its receptor (AR) in promoting melanoma aggressiveness. However, underlying cellular and molecular mechanisms and their precise pathological contributions are not well-defined. We recently discovered a sex-associated disparity in melanoma fucosylation, the post-translational modification of proteins with the dietary sugar L-fucose. Fucosylation, the conjugation of fucose moieties onto different glycan linkages on target …

“You Are Not The Same Person You Were:” On Diagnosis Seeking During A Liminal Period And Systemic Lupus Erythematosus, Kaylee A. Appleton

USF Tampa Graduate Theses and Dissertations

“You are the healthiest looking sick person I’ve ever met”

(Daphne, 62)

Systemic Lupus Erythematosus (SLE) is one of many chronic illnesses considered to be “invisible.” Once the onset of symptoms occurs, the illness can drastically change the dynamic of an individual's life in ways that may not be noticeable to others, even before a chronic illness diagnosis. I discuss how these individuals experience the liminal period of developing symptoms and seeking a diagnosis. Furthermore, I discuss vignettes that exemplifies the occurrence of a liminal state while looking at the current for-profit set-up of healthcare in the United States. Utilizing …

Elucidating The Role Of The Tumor Suppressor Gene Rna-Binding Motif 5 (Rbm5) In Models Of Traumatic Brain Injury, Kara L. Snyder

USF Tampa Graduate Theses and Dissertations

Traumatic brain injury (TBI) is a worldwide health burden that causes death and disability. Clinical management of TBI involves guideline-based protocols to limit secondary brain injury but there remains an unmet need for therapeutics to target neuroprotection and to improve cognitive outcomes in patients. This dissertation concerns the examination of RNA binding motif 5 (RBM5) as a potential therapeutic gene target to promote neuroprotection in models of TBI. RBM5 is a potent pro-death tumor suppressor gene in cancer. We hypothesized that it also promotes cell death in neurons in the brain and therefore its inhibition would decrease neuropathology following CNS …

The Role Of Muslim Women In Nigeria’S Socioeconomic Development Through The Implementation Of The United Nations Sustainable Development Goals, Faqiat Afolake Adeaga

USF Tampa Graduate Theses and Dissertations

This research explores the often underestimated yet pivotal role of Muslim women in propelling socio-economic development in Nigeria, specifically focusing on the implementation of the United Nations Sustainable Development Goals (SDGs). The primary research question seeks to comprehend the transformative impact of faith-based women-led NGOs, including Ansar-ud- Deen, Nawair-ud-Deen, FOMWAN, and NASFAT, on Muslim women's empowerment and their contribution to SDG1 (extreme poverty), SDG3 (health), SDG4 (education), and SDG5 (gender equality) in Nigeria. Simultaneously, the secondary research question explores successful strategies employed by these organizations in empowering Muslim women, with a keen focus on potential areas for improvement. Incorporating research …

Comparing The Effects Of The Comprehensive School Threat Assessment Guidelines Training Workshop: Online Vs. In-Person, Caroline Mierzwa

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to examine participant outcomes related to the Comprehensive School Threat Assessment Guidelines (CSTAG) professional development training workshop when delivered in-person and online. Participant outcomes in knowledge of threat assessment, ability to classify a threat case appropriately, and overall evaluations of the training workshop were examined to evaluate threat assessment team aspirations to use the threat assessment procedures and effectiveness of the CSTAG training. Kirckpatrick's Model of Evaluation was utilized to investigate participants' reactions to the training and learning outcomes. Furthermore, participant results were assessed based on their respective position within schools, encompassing roles such …

Examining Associations Between Social And Emotional Loneliness And Drinking In A Clinical Sample With Alcohol Use Disorder (Aud): An Ecological Momentary Assessment Study, Brendan E. Walsh

USF Tampa Graduate Theses and Dissertations

Loneliness, a negative affective state that arises when someone perceives a lack of social connectedness, is a predisposing and maintaining factor of alcohol use behavior. Several studies have linked loneliness to drinking at the daily level and elevated AUD risk using longitudinal designs; however, cross-sectional studies have identified inconsistent patterns. The current study applied a multidimensional framework of loneliness (i.e., emotional and social dimensions) to examine daily relations between loneliness and drinking in an AUD sample (N = 60), with consideration of drinking context (social vs. solitary) as a moderator. Results indicated that emotional loneliness (within-person) was associated with increased …

Development Of A Suspended Aerobic Membrane Bioreactor (Sambr) For Sustainable Wastewater Treatment On Early Planetary Bases, Talon James Bullard

USF Tampa Graduate Theses and Dissertations

Environmental Control and Life Support Systems (ECLSS) ideally should treat all “waste” streams for recovery in a sustainable and near closed-loop manner. This will be critical for Partial Gravity Habitats (PGH) and Early Planetary Bases (EPB) that will be established on the Moon and Mars due to the lack of readily available resources. Currently, the Water Recovery System (WRS), onboard the International Space Station (ISS), recovers and purifies water from urine and graywater. While efficient, this system is not optimized for future missions and utilizes physical-chemical technologies that operate with single-use and hazardous consumables. In response to the lack of …

Using Repeated-Latency Measures To Assess Functional Relationship, Justin B. Han

USF Tampa Graduate Theses and Dissertations

Functional analysis (FA) is the gold standard methodology for identifying maintaining variable of a target behavior in the field of behavior analysis. Although behavior analysis generally accepts that it is the most accurate behavior assessment method, it is still underutilized in clinical settings (e.g., Oliver et al., 2015; Roscoe et al., 2015). To address this concern, previous researchers have investigated the barriers to implementation such as training (e.g., Hay-Hansson & Eldvik, 2013; Iwata et al., 2000; Wacker et al., 2013), risks involved (e.g., Call et al., 2013, 2017; John, 2019; Shabani et al., 2013), and length of assessment (Saini et …

Methods For Analytical And Biophysical Analysis For Investigation Of Stimulator Of Interferon Genes In Drug Discovery, William Emmett Brian Lawless

USF Tampa Graduate Theses and Dissertations

This work elaborates on analytical and biophysical techniques including high pressure liquid chromatography (HPLC), mass spectrometry quadrupole time-of-flight (MS-QToF), dynamic light scattering (DLS), microscale thermophoresis (MST), surface plasmon resonance (SPR), isothermal calorimetry (ITC), size exclusion chromatography, and native gel electrophoresis. A basic overview is described for each technique and is designed for easy understanding by both novice and experienced analysts. Best practices for each technique are discussed and suggestions are made for protocol improvements or troubleshooting. Protocol development is discussed, and a method is introduced for MST that can be adapted to any target protein for rapid screening of analog …

The Contributions Of Mental Health Issues, Traumatic Brain Injury, And Adverse Childhood Experiences To Recidivism Among Rural Jail Incarcerees, Lauren N. Miley

USF Tampa Graduate Theses and Dissertations

Recidivism should be a major concern, particularly for American jails. The Unites States incarcerates 2.3 million people, a number exceeding rates in any other country. Approximately 90% of those individuals will reenter society at some point, and roughly half of those people will ultimately return to jail. This revolving door costs U.S. taxpayers upwards of $39 billion per year, and undermines public safety. Therefore, breaking this cycle of incarceration and recidivism is of utmost concern, and the focus of this dissertation. Mental health problems, traumatic brain injury (TBI), and adverse childhood experiences (ACEs) are three potential risk factors for recidivism …

Algorithmic Design In Recommender Systems: Expanding Dbe Participation In The U.S. Construction Industry, Dick C.E. Davis Ii

USF Tampa Graduate Theses and Dissertations

The multifaceted U.S. construction environment, characterized by vast projects and intricate agreements, is anchored in trust, exemplified by bonding. For Disadvantaged Business Enterprises (DBEs), the bonding process can be challenging.

This dissertation provides an in-depth evaluation of the "System for facilitating a project between contractors and owners," a patented methodology innovatively developed by my father, Dr. Dick Davis, Sr. and later approved through my research efforts, as indicated by the patent number US8346582B1. The system adeptly gathers and processes data from owners, projects, and contractors. Despite its proficiency in managing extensive contractor and project data, the system faces challenges in …