Digital Commons Network^™

Quantitative Proteomic Investigation Of Disease Models Of Type 2 Diabetes, Mark Gabriel Athanason

USF Tampa Graduate Theses and Dissertations

PANcreatic DERived factor (PANDER, FAM3B) is a member of a superfamily of FAM3 proteins that are uniquely structured and strongly expressed from the endocrine pancreas and co-secreted with insulin. Unique animal models available to our lab have indicated that PANDER can induce a selective hepatic insulin resistant (SHIR) phenotype whereby insulin signaling is blunted yet lipogenesis is increased. The complexity of the biological networks involved with this process warranted the logical approach of employing quantitative mass spectrometry based proteomic analysis using stable isotope labeling of amino acids in cell culture (SILAC) to identify the global proteome differences between the PANDER …

The Effects Of Synthetic And Dietary Therapeutics On Learning, Memory, Motor Coordination, And Seizure In An Angelman Syndrome Mouse Model, Stephanie Lynn Ciarlone

USF Tampa Graduate Theses and Dissertations

Angelman syndrome (AS) is a rare genetic and neurological disorder presenting with severe developmental delay, ataxia, epilepsy, and lack of speech. AS is associated with a neuron-specific loss of function of the maternal UBE3A allele, a gene encoding an E3 ubiquitin ligase. Currently, no cure exists for this disorder; however, recent research using an AS mouse model suggests that pharmacological intervention is plausible, and can alleviate some of the detrimental phenotypes reported in AS patients.

Although there is no curative treatment for AS, seizure medication and behavioral therapies are most commonly prescribed in order to minimize symptoms. However, these options …

Towards Computational Human Behavior Modeling For Just-In-Time Adaptive Interventions, Tylar Murray

USF Tampa Graduate Theses and Dissertations

The advent of powerful wearable devices and smartphones has enabled a new generation of “in-the-wild” user studies, adaptive behavioral intervention strategies, and context measurement. Though numerous proof-of-concept studies continue to push the limitations of what a behavioral scientist can do with these technologies, there remains a major methodological roadblock separating behavioral theory and application. Avatar-user interaction theory, for example, is not well defined in its formulation, and thus guidelines for intervention designers depend on heuristic methods and designer intuition. Computational modeling has been slow to move into behavioral science in general, but a growing population of behavioral scientists recognize this …

Climate Change Drives Outbreaks Of Emerging Infectious Disease And Phenological Shifts, Jeremy Cohen

USF Tampa Graduate Theses and Dissertations

Climate change is expected to impact species by altering infectious disease outcomes, modifying community composition, and causing species to shift their phenology, body sizes and range distributions. However, the outcomes of these impacts are often controversial; for example, scientists have debated whether climate change will exacerbate emerging infectious disease and which species are at greatest risk to advance their phenology. There reason for these controversies may be that climate change is impacting diverse processes across a wide range of ecological scales, as the interplay between fine-scale processes and broad-scale dynamics can often cause unpredictable changes to the biosphere. Therefore, it …

Hypercapnic Hyperoxia Increases Free Radical Production And Cellular Excitability In Rat Caudal Solitary Complex Brain Slice Neurons, Geoffrey Edward Ciarlone

USF Tampa Graduate Theses and Dissertations

The caudal solitary complex (cSC) is a cardiorespiratory integrative center in the dorsal medulla oblongata that plays a vital role in the central CO₂-chemoreceptive network. Neurons in this area respond to hypercapnic acidosis (HA) by a depolarization of the membrane potential and increase in firing rate, however a definitive mechanism for this response remains unknown. Likewise, CO₂-chemoreceptive neurons in the cSC respond to hyperoxia in a similar fashion, but via a free radical mediated mechanism. It remains unknown if the response to increased pO₂ is merely an increase in redox signaling, or if it’s the …

The Apocalypse Narrative And The Internet: Divided Relationships In New Natures, Brooks Scott Benadum

USF Tampa Graduate Theses and Dissertations

This project proposes that one factor of growing societal interest in the apocalypse narrative is rooted in these stories reflection on our new landscape of telecommunication flows embodied in the Internet. The apocalypse narrative has steadily been growing in popularity, and many academics have offered potential explanations. While other analyses predominately focus on the actual apocalyptic event itself as representative of various societal fears, this project aims to focus on aspects of how we adapt to being in the new apocalyptic landscape, and how this reflects on our own adaptation to being in the new landscape of the Internet. This …

Expansion Of The Performance Capabilities Of The Usf Inhalation Challenge Chamber, Laura Riley

USF Tampa Graduate Theses and Dissertations

The purpose of this research was to evaluate the capability and performance of the University of South Florida’s (USF) Human Exposure Chamber (HEC) using aerosols in the thoracic range. The goals of this research were two-fold: to obtain an average particle size of 10 µm (thoracic-size range) inside the chamber during dust production and to test for evenness of dust concentration within the chamber. The USF HEC can be used for studies using gases and/or particulates. The chamber measurements are 4.16 ft x 2.67 ft x 6.75 ft, for a total volume of 75 ft³ or 2.13 m^{3 …}

The Role Of Religion In Mitigating Cancer Disparities Among Black Americans, Samar Hennawi

USF Tampa Graduate Theses and Dissertations

The long history of racism has created cultural barriers that prevent some Black Americans from seeking cancer treatment. Fatalism, physician mistrust, low levels of social support and self-efficacy, are the most cited cultural barriers in the literature. Black Americans’ religious beliefs and church involvement have historically helped them in their struggle against racism. A quantitative and a qualitative comparison studies examine the role of Black cancer survivors’ religious beliefs in their fight against cancer. The quantitative comparison study finds no difference in the cultural attitudes between Black and White cancer survivors. However, the qualitative comparison study between the same two …

The Guilty But Mentally Ill Verdict: Assessing The Impact Of Informing Jurors Of Verdict Consequences, Erin Elizabeth Cotrone

USF Tampa Graduate Theses and Dissertations

In response to public opposition to the insanity defense, the Guilty But Mentally Ill(GBMI) verdict was enacted with the intention of limiting the number of insanity acquittals and alleviating the public’s concerns. Prior research suggests, however, that many jurors are making verdict decisions with limited knowledge of the dispositional consequences of the GBMI and NGRI verdicts. Further, jurors may erroneously assume that the GBMI verdict is a compromise between a NGRI and guilty verdict, which mitigates punishment. In reality, the dispositional consequences of a GBMI verdict are equivalent to or more restrictive than a guilty verdict. The current study examined …

Virtual Reality Serious Games For Individuals With Autism Spectrum Disorder: Design Considerations, Lal Bozgeyikli

USF Tampa Graduate Theses and Dissertations

Virtual reality has been a popular training tool for individuals with Autism Spectrum Disorder (ASD) in recent years. Although virtual reality was proven to be a promising tool for individuals with ASD by many previous studies, effects of virtual reality properties on user experience is still an unexplored area. More comparison studies and reliable data are needed to identify the benefits of different VR methods and properties, and leverage the future VR systems. In this dissertation, we explored effects of virtual reality properties on user experience of high functioning individuals with ASD with four different serious game experiments. The first …

Intraocular Pressure Sensing And Control For Glaucoma Research, Simon Antonio Bello

USF Tampa Graduate Theses and Dissertations

Animal models of ocular hypertension are important for glaucoma research but come with experimental costs. Available methods of intraocular pressure (IOP) elevation are not always successful, the amplitude and time course of IOP changes are unpredictable and irreversible, and IOP measurement by tonometry is laborious. This dissertation focuses on the development and implementation of two novel systems for monitoring and controlling IOP without these limitations. The first device consists of a cannula implanted in the anterior chamber of the eye, a pressure sensor that continually measures IOP, and a bidirectional pump driven by control circuitry that can infuse or withdraw …

Hormone Replacement Therapy (Hrt) Modulates Peripheral And Central Auditory System Processing With Aging, Tanika Williamson

USF Tampa Graduate Theses and Dissertations

After the findings were reported for the Women’s Health Initiative (WHI) study in the past decade, there has been a significant decline in the overall use of hormone replacement therapy (HRT) among women. However, there are still millions of middle-aged, menopausal women in the U.S. who are currently undergoing hormone therapy. Their reasons for continuing treatment include relief of severe menopausal symptoms, aid in the management of osteoporosis and reduction in the risk of colon cancer (Ness et al., 2005). The purpose of the following investigation was to evaluate the impact of HRT on the central and peripheral auditory systems …

Comparison Of Prehistoric And Protohistoric Components At The Lighthouse Bayou Shell Midden, 8gu114, Northwest Florida, Theodore Gold Gold

USF Tampa Graduate Theses and Dissertations

The dawn of the eighteenth century in the Apalachicola delta region of the Florida panhandle was a time of major social upheaval that has been underexplored by current research. There are no historic records that describe the events and peoples in the region during establishment of the Spanish missions in the Tallahassee area to the east. Archaeological evidence shows the disappearance of the late prehistoric Mississippian Fort Walton people and the brief emergence of the protohistoric Lamar archaeological culture during the time of the destruction of the Spanish mission system around 1704. The Lighthouse Bayou site, 8Gu114, in Gulf County, …

Molybdenum Disulfide-Conducting Polymer Composite Structures For Electrochemical Biosensor Applications, Hongxiang Jia

USF Tampa Graduate Theses and Dissertations

Lactic acid is widely existing in human bodies, animals and microorganisms. Recently, using biosensor to detect the concentration of lactic acid and diagnose disease have attracted great research and development interests. Nanocomposites is one of the best material used for biosensor because their wonderful conductivity, optical and electrochemical properties. In the study, MoS₂ and polypyrrole (PPY) are used for the composite material electrode. To determine whether lactate oxidase (LOD) was helpful for the biosensor’s detective properties, both PPY-MoS₂ film with LOD and PPY-MoS₂ film without LOD are being tested. The fourier transform infrared spectroscopy (FTIR) and Raman …

The Other Earthquake: Janil Lwijis, Student Social Movements, And The Politics Of Memory In Haiti, Laura A. Leisinger

USF Tampa Graduate Theses and Dissertations

Among increased calls for "new narratives" of Haiti, this thesis seeks to honor Haitian traditions of intellectualism and resistance, centering on the life and legacy of martyred professor Janil Lwijis in post-earthquake student social movements. Based on oral histories with student activists at the State University of Haiti (UEH), this work explores student protest in Haiti through the voices, often at odds, of those en lutte; it explores how Janil is invoked and remembered, and argues that oral history can contribute to activist research and pose a challenge to dominant narratives. A legacy that is contested, differential claims to Janil's …

The Influence Of Oxygen Tension And Glycolytic And Citric Acid Cycle Substrates In Acrolein-Induced Cellular Injury In The Differentiated H9c2 Cardiac Cell Model, Jayme Coyle

USF Tampa Graduate Theses and Dissertations

Most in vitro systems employ the standard cell culture maintenance conditions of 95 % air with 5 % CO2 to balance medium pH, which translates to culture oxygen tensions of approximately 20 % - above the typical ≤ 6 % found in most tissues. The current investigation, therefore, aims to characterize the effect of maintenance and toxicant exposure with a particular focus on the α,β-unsaturated aldehyde, acrolein, in the presence of physiologically relevant oxygen tension using a differentiated H9c2 cardiomyoblast subclone. H9c2 cells were maintained separately in 20.1 and 5 % oxygen, after which cells were differentiated for five days, …

Teaching Culinary Skills Using Video Modeling To Individuals With Traumatic Brain Injury, Sarah Castro

USF Tampa Graduate Theses and Dissertations

Individuals with Traumatic Brain Injury (TBI) often need to be taught independent living skills in order to reintegrate into community settings. This study examined the use of video modeling to teach culinary skills to three individuals with TBI. Video modeling is easily accessible, inexpensive, and not reliant on an additional person to directly teach skills. For all three participants, video modeling resulted in increases in cooking skills using a task analysis created for each food item prepared. For one participant, the skills maintained over two weeks and generalized to a novel food. For another participant video modeling was insufficient in …

Pepper Mild Mottle Virus As A Surrogate For Enteric Viruses: Implications For Assessing Water Quality, Erin Michelle Symonds

USF Tampa Graduate Theses and Dissertations

Less than 10% of the world’s domestic wastewater is disinfected prior to discharge into surface waters; therefore, human exposure to diverse wastewater-related pathogens results in millions of cases of illness each year. Among the enteric pathogens, viruses represent an important group of emerging pathogens and are frequently the cause of food- and water-borne outbreaks of illness. Although the World Health Organization and many government agencies mandate the use of bacterial indicators to identify poor microbial water quality, it is well known that these indicators poorly correlate with fecal pollution contamination events and risk of disease. The field of public health-related …

Formation Of Trihalomethanes (Thms) As Disinfection By-Products (Dbps) When Treated Municipal Wastewater Is Disinfected With Sodium Hypochlorite, Helene Kassouf

USF Tampa Graduate Theses and Dissertations

Disinfection is an essential process in the treatment of municipal wastewater before the treated wastewater can be discharged to the environment. Hillsborough County's Northwest Regional Water Reclamation Facility (NWRWRF) in Tampa, Florida, currently uses ultraviolet (UV) light for disinfection. However, this method has proven expensive to implement and maintain, and may not be effective if the light transmission is poor. For these reasons, Hillsborough County is considering switching from UV light to sodium hypochlorite for disinfection. However, hypochlorite (chlorine) disinfection has disadvantages as well, such as the production of disinfection by-products (DBPs) such as trihalomethanes (THM) and haloacetic acids (HAAs), …

A Habitat Analysis Of Estuarine Fishes And Invertebrates, With Observations On The Effects Of Habitat-Factor Resolution, Brianna Michaud

USF Tampa Graduate Theses and Dissertations

Between 1988 and 2014, otter trawls, seine nets, and plankton nets were deployed along the salinity gradients of 18 estuaries by the University of South Florida and the Florida Fish and Wildlife Research Institute (FWRI, a research branch of the Florida Fish and Wildlife Conservation Commission). The purpose of these surveys was to document the responses of aquatic estuarine biota to variation in the quantity and quality of freshwater inflows that were being managed by the Southwest Florida Water Management District (SWFWMD).

In the present analyses, four community types collected by these gears were compared with a diversity of habitat …

Comparing Game Simulation To Concept Models For Student-Centered Learning In Biology, Margaurete Romero

USF Tampa Graduate Theses and Dissertations

Science education research continues to demonstrate improved learning with active-learning techniques compared to lectures. However, the question of which active-learning methods are the most effective for learning complex scientific principles in various context still remains. Models are commonly used in activities that allow students to simplify complex systems and understand how components interact. I investigated the outcomes for student learning and engagement of two model-based activities - concept models and game simulations. The activities were conducted in an introductory biology course in sixteen discussion sections. Eight sections were assigned to the concept model activity and eight to the simulation activity. …

Corrosion Characteristics Of Magnesium Under Varying Surface Roughness Conditions, Yahya Efe Yayoglu

USF Tampa Graduate Theses and Dissertations

The biggest challenge with magnesium alloy biodegradable implants is the rapid corrosion at the earlier stages of the healing process after implantation. In this research, the impact of surface roughness generated by different means on the corrosion rate of AZ31 magnesium alloy in a simulated biologic environment is investigated. In order to perform accurate experimentation, an in vitro setup is assembled that simulates the human body environment accurately has been prepared using Schinhammer’s in vitro immersion testing setup and Kokubo’s Simulated Body Fluid (SBF). For the immersion test of Mg in SBF, several surface texture groups of Mg have been …

Investigating Early Village Community Formation And Development At Kolomoki (9er1), Shaun Eric West

USF Tampa Graduate Theses and Dissertations

In southeastern North America, the Woodland period (ca. 1000 B.C. to A.D. 1050) was arguably witness to the first early village societies, and Kolomoki—located in southwestern Georgia—is among the largest villages during this interval. Though archaeologists recognize these communities as seminal developments in the course of human history, little attention has been paid to how they develop and vary internally. This thesis seeks to address these issues by focusing on the development and social construction of the early village community at Kolomoki. The results of an excavation program carried out within Kolomoki’s South Village affords a clearer picture of this …

Resource Use Overlap In A Native Grouper And Invasive Lionfish, Joseph Schmidt Curtis

USF Tampa Graduate Theses and Dissertations

Invasive species can severely disrupt biological communities through their interactions with native organisms, yet little is known about the response of marine predators to the establishment of a competitive invasive fish. In the western Atlantic, invasive Indo-Pacific lionfishes (Pterois spp.) may represent a novel competitor to several commercially and ecologically important native species. However, there is a scarcity of empirical research documenting comparative resource use of cohabitant lionfish and native fishes, as well the physiological consequences that may result from interspecific interactions with the invasive species. For this thesis, I conducted two studies designed to elucidate the strength …

The Impact Of Emojis And Emoticons On Online Consumer Reviews, Perceived Company Response Quality, Brand Relationship, And Purchase Intent., Jayme Hill Hill

USF Tampa Graduate Theses and Dissertations

Several studies have examined the use of emoji and emoticons in computer mediated communication among peers and colleges but there is yet academic research on the impact of businesses using these paralinguistic cues when responding to online consumer reviews. This research is examining the influence these paralinguistic cues have on the consumers perception of the companies quality of the response to an online consumer review, brand relationship, purchase intent.

Using an online survey, participants are asked to answer general questions about the brand, the quality of the response, brand relationship, and purchase intent after seeing a random condition of both …