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Analysis Of Adverse Events Associated With Sglt2 Inhibitors And Molecular Mechanisms Using Faers Database And Transcriptome Data, Rahinatu Bawah Ali

USF Tampa Graduate Theses and Dissertations

In this thesis, we explored the differential effects of sodium-glucose cotransporter-2 (SGLT2) inhibitors on cardiovascular and renal health, utilizing both clinical data and molecular insights. Focusing on three commonly prescribed SGLT2 inhibitors—Dapagliflozin, Canagliflozin, and Empagliflozin—we employed the Food and Drug Administration Adverse Event Reporting System (FAERS) database to assess adverse events, including myocardial infarction, cardiac failure, tachycardia, acute kidney injury (AKI), renal failure, urinary tract infections, and fungal infections. This analysis was complemented by transcriptomic data from kidney cell lines treated with these drugs, processed through NCBI’s GEO2R and Limma programs, to reveal the gene expression profiles linked to these …

Moral Of The Story: Secondary Language Arts Teachers’ Perceptions Of Students’ Moral Intelligence, Sadia Fauzia Zaman

USF Tampa Graduate Theses and Dissertations

The literature review reveals a gap in secondary English Language Arts (ELA) teachers’ perception of students’ moral intelligence (MI). While many character education studies examine the student perspective, this study contributes to the limited research on secondary ELA teachers’ perceptions of students’ moral intelligence and how it can be cultivated. This dissertation addresses that gap by collecting and analyzing ELA teachers’ lived experiences and perceptions of students’ MI, as well as exploring how ELA teachers and schools can support students’ moral development by integrating moral and ethical topics into instructional practices. This qualitative study used narrative inquiry to gather interview …

Preservice Elementary Teachers’ Perceptions Of The Incorporation Of An Ecojustice Framework In An Undergraduate Stem Integration Course, Rita G. Ortiz

USF Tampa Graduate Theses and Dissertations

Environmental education across the K-12 science curriculum lacks critical examination of human and ecological relationships, particularly in relation to science, technology, engineering, and mathematics (STEM) education. While the Next Generation Science Standards include environmental topics within their disciplinary core ideas and many schools offer environmental science courses, this gap can be addressed in teacher preparation programs, particularly for those teaching younger students. Ecojustice centers on socioecological topics by addressing cultural assumptions we, as humans, live by in relation to natural systems. Ecojustice education is a critical pedagogy that engages learners about social issues related to our current ecological crisis. This …

Can The Effluent From Wastewater Stabilization Ponds Be Effectively Managed For Health And Nutrient Recovery?, Michael Glass

USF Tampa Graduate Theses and Dissertations

Wastewater stabilization ponds, such as facultative and maturation lagoons, have been shown to be effective processes to manage conventional water quality parameters found in domestic wastewater. The main goal of this thesis is to explore whether wastewater stabilization ponds can simultaneously reduce pathogens to safe levels while supporting agricultural activities through nutrient recovery. A large dataset for water quality of wastewater lagoon effluent in the U.S. was obtained at the state level and combined into regional southern and northern locations. Analysis of data showed there was significant difference in lagoon effluent concentration between northern and southern states for the water …

Monsters, Multiplicities, And Liminal Experiences In Doctoral Education: Fabulation As Research Methodology, Anna V. Gonzalez

USF Tampa Graduate Theses and Dissertations

The purpose of this dissertation was to experiment with qualitative research methodologies while exploring the complexities of doctoral education, research practices, philosophies of inquiry, and other topics intersecting with(in) educational scholarship. My vision for this work was fueled by postmodernist critiques of humanist education, frustrations with the methodological status quo pervasive in educational research, and deep concerns about the unsustainability of performance-focused teaching and learning.

The logic I follow in this project is simple: if we—researchers and academics—are to contribute solutions for challenges and ongoing crises in our world, then we must start thinking relationally and in ways more complex …

Spatiotemporal Dynamics Of Coastal Nekton Metacommunities In The Western Atlantic, Jonathan A. Peake

USF Tampa Graduate Theses and Dissertations

The processes of diversification, selection, dispersal, and stochastic drift can combine in many ways to assemble ecological communities. A key focus in ecology is to describe and quantify the relative influences of these processes for different systems and explain the mechanisms by which these processes produce patterns of community structure. To accomplish this goal, ecologists have developed several frameworks that seek to integrate processes that occur locally andregionally. The metacommunity concept treats local communities as pieces of a broader landscape that are connected through dispersal of their constituent organisms. Empirical studies have generally lagged behind the development of metacommunity theory. …

Evaluating The Effectiveness Of Edge Line Sinusoidal Rumble Strips On Lane Departure Crash Prevention And Injury Severity, Shubhankar Chintamani Shindgikar

USF Tampa Graduate Theses and Dissertations

Lane departure crashes are one of the most prevalent accidents in Florida and the United States. Various forms of rumble strips have been employed to mitigate and decrease these incidents. The Florida Department of Transportation (FDOT) recently adopted a new sinusoidal rumble strip pattern for statewide implementation of audible and vibratory treatment (AVT). Recent research indicates sinusoidal rumble strips are highly effective in alerting drivers audibly and through tactile vibrations, thus aiding lane departure prevention. Moreover, they generate less external noise compared to other rumble strip textures.

However, a systematic assessment of the safety effectiveness of sinusoidal rumble strips in …

Phosphoglycerate Dehydrogenase Regulated By The Mitogen-Activated Protein Kinase Pathway Is An Actionable Vulnerability In Cutaneous Melanoma, Neel Jasani

USF Tampa Graduate Theses and Dissertations

Metabolic alterations play a critical role in shaping tumor growth and development. Many metabolic enzymes are shown to play an important role in different aspects of tumor development, growth, and metastasis. One such enzyme that we are investigating in this project is the Phosphoglycerate Dehydrogenase (PHGDH). The PHGDH gene, encoding the rate-limiting enzyme of the serine synthesis pathway, is located at chromosome locus 1p12 and frequently amplified in melanoma. PHGDH is required for the proliferation of melanoma cells with chromosome 1p12 amplification. However, the role of PHGDH in chromosome 1p12 non-amplified melanoma is poorly understood. In this project, we sought …

Forgotten Partners: Mothers’ Perceptions Of Early Literacy Instruction For Students With Intellectual And Developmental Disabilities, Andrew Payne

USF Tampa Graduate Theses and Dissertations

Families are often the forgotten partners in education and in educational research. It is important to consider input from families regarding their children’s education. Their voices are crucial ones that should be heard more often. The research is currently lacking information about families’ perceptions about the education of their children with significant disabilities. This qualitative study explored mothers’ perspectives on the literacy education of their children and the role they play in the process. Stories of five participants and their lived experiences of supporting their children with intellectual and development disabilities in acquiring early literacy skills are shared. Themes relating …

On The Role Of Prediction In Streaming Hierarchical Learning, Ramy Mounir

USF Tampa Graduate Theses and Dissertations

In today's world, AI systems need to make sense of large amounts of data as it unfolds in real-time, whether it's a video from surveillance and monitoring cameras, streams of egocentric footage, or sequences in other domains such as text or audio. The ability to break these continuous data streams into meaningful events, discover nested structures, and predict what might happen next at different levels of abstraction is crucial for applications ranging from passive surveillance systems to sensory-motor autonomous learning. However, most existing models rely heavily on large, annotated datasets with fixed data distributions and offline epoch-based training, which makes …

Assessing The Impact Of Ai Assisted Software Development And User Experience Of A College Football Simulation Game: A Study Of Player And Industry Professional Perspectives, Augustus J. Scarlato Iii

USF Tampa Graduate Theses and Dissertations

This research examines the use of Artificial Intelligence (AI) in the design of video games, specifically the development of a college football simulation game. This study documents the creation of an alpha version college simulation game assisted by Open AI’s Chat GPT 4.0 API, to potentially improve game development, gameplay realism, and user interaction. The study then assesses how both student players and industry professionals perceive AI-enhanced gaming, emphasizing the usability, gameplay experience, and overall quality of the game using a Likert scale survey. The analysis also highlights differences in perceptions between students and industry professionals, with the latter group …

Selective Pericyte Recruitment To Ec-Lined Tubes, Vessel Assembly Enhancement Through Pericyte Timp-3 Induction, And Pericyte Tbx2 In Capillary Formation And Maturation, Ksenia Marilyn Yrigoin Kaluguina

USF Tampa Graduate Theses and Dissertations

Capillaries, the smallest blood vascular networks, are formed by narrow and elongated endothelial cell (EC)-lined tubes with associated pericytes anchored to the abluminal tube surface. Our laboratory demonstrated that dynamic interactions between ECs and pericytes during capillary tube network assembly are required for basement membrane matrix deposition and maturation. We have also shown that key EC-derived factors promote pericyte recruitment, proliferation, and pericyte-induced basement membrane formation during capillary assembly. In the first section, we aim to identify if vascular smooth muscle cells (VSMCs) respond to the same factors and have the same invasive ability as pericytes since they are both …

Rictor, A Mtorc2 Protein, Regulates Lymphatic Valve Formation And Maintenance Through The Akt-Foxo1 Signaling Axis, Richa Banerjee

USF Tampa Graduate Theses and Dissertations

The lymphatic vasculature maintains tissue fluid homeostasis by absorbing interstitial fluid to form lymph and transporting it back to the bloodstream. Collecting lymphatic vessels have lymphatic valves (LVs) that prevent the retrograde flow of lymph generated by hydrostatic pressure from the blood vasculature. Backflow and accumulation of lymph leads to a lymphatic pathology called lymphedema. This implicates the involvement of defective LVs in the pathogenesis of lymphedema and warrants the study of lymphatic valve development and maintenance. The LVs are formed through a process called mechanotransduction which signals through the intracellular PI3K-AKT pathway in lymphatic endothelial cells. However, the activation …

The Pilling Process: Significance Quest, Self-Verification, And Fusion In Online Incel Forums, Gregory J. Rousis

USF Tampa Graduate Theses and Dissertations

Incels (short for “involuntary celibates) are an online community of men who blame women for their reputed sexual and romantic failures. The misogyny of their worldview, called the blackpill, has inspired multiple instances of real-world violence against women. Alarmingly, we know little about the temporal processes whereby an incel goes from joining an online community to becoming deeply aligned (i.e., “fused”) with the group. This project sought to address this by scraping incels’ most popular web forum and using text analysis to identify psychological mechanisms that could be responsible for incels’ fusion. The resulting dataset consisted of 1,097,841 unique discussion …

Approach-Avoidance Action Patterns As Indicators Of Empathic ‘Care Drive’ Strength, Stephanie R. Hruza

USF Tampa Graduate Theses and Dissertations

Empathy, care, and prosocial behavior are essential to social group cohesion, and antisocial and violent criminal behavior, on the other pole, can cause serious disruptions in group cohesion and negatively impact society as a whole. Different psychopathological conditions tend to present with impairments in affective empathy/caring and prosocial behavior, as is observed in psychopathic traits, which are tied to antisocial behavior and outcomes. It is essential to understand how empathy and care function, both in psychopathology and normal prosocial functioning, in order to inform interventions for empathy/care deficits and avoid the potential harm that such deficits can cause for others …

Regioselective Hydroamination Of Unactivated Olefins With Diazirine And Total Synthesis Of Nostodione A And Scytonemin, Qingyu Xing

USF Tampa Graduate Theses and Dissertations

As an atom-economic way of carbon-nitrogen bond formation, hydroaminations are great tool for make amines, hydrazines, and other nitrogen-containing heterocycles. Specifically, radical hydroamination is a useful approach for functionalization of unactivated olefins, especially in sterically hindered environments. Diversification remains a challenge for radical hydroaminated products, since the nitrogen sources are carefully designed. In the first chapter of this dissertation, we report a highly diversifiable radical hydroamination reaction with diazirine as a nitrogen source and its potential applications in synthesis of several pharmaceutical interested compounds. Cobalt catalyzed regioselective hydroamination with diazirine shows wide function group tolerance, like phenyl thioether, ketones, esters, …

An Investigation Into Loneliness And Solitary Drinking In A University Sample, Daniel C. Faraci

USF Tampa Graduate Theses and Dissertations

Alcohol consumption tends to occur in social contexts and has been shown to increase prosocial behaviors and facilitate social connection. Simultaneously, socially excluded drinkers tend to increase or continue problematic levels of alcohol consumption when alone, possibly consuming alcohol as a replacement for desired but unavailable social connection. This investigation attempted to examine the relationship between loneliness (i.e., lack of perceived social connection) and subsequent alcohol consumption in solitary versus social contexts using ecological momentary assessment (EMA) techniques. University students recruited through a research subject pool who drank at least once per week were asked to respond to 28 short …

Teachers' And School Directors' Perceptions Of The Benefits Of Arts-Integrated Instruction K-2: Before, During, And After Covid-19, Alison H. Mehler

USF Tampa Graduate Theses and Dissertations

The purpose of this study is to explore the experiences and perceptions of teachers who use arts-integrated instruction and their directors of K-2 students before, during, and after the unique period of online instruction during the COVID-19 pandemic, which began in March of 2020. Arts integration is a method of teaching in which students are taught comprehension of subject areas (i.e. – math, science, reading, social studies) through art forms. These art forms include music, theatre, dance, art, and writing. I collected he qualitative data for this research through virtual interviews with five female participants. I transcribed these interviews and …

The Application Of Multi-Isotope Analysis To Assist In Geoprofiling Unidentified Decedents Within Florida, Liotta D. Noche-Dowdy

USF Tampa Graduate Theses and Dissertations

The biochemistry of human tissues can provide information for geoprofiling (geographic birthplace or migration patterns) of an unidentified decedent. Utilizing isotope analyses (both stable and radiogenic) from teeth, bone, nails, and/or hair reveals specific isotopic information that correlates to diverse geographic regions reflecting an individual’s birthplace and migration patterns. Isotope analysis of enamel and bone has been employed in bioarchaeology for decades to reconstruct the diet, health, and migration patterns of past populations. Isotope ratios measured from enamel can then be compared to isotope ratios measured from bone of the individual to track patterns of migration. The enamel from the …

Evaluating When Changes In Delay Discounting Occur In A Residential Alcohol Treatment Program, Asha A. Fuller

USF Tampa Graduate Theses and Dissertations

Delay discounting has long been associated with substance misuse across both animal subjects and human participants. The purpose of the proposed study was to evaluate if and when changes in delay discounting occur during a residential treatment program for alcohol use. Participants were asked to complete the 5-trial adjusting amount discounting task (Koffarnus & Bickel, 2014) and an electronic version of the Du et al. (2002) delay discounting task repeatedly across 21 days while in residential treatment. Additional assessments included the Alcohol Use Disorder Identification Test (AUDIT; Saunders et al., 1993), a demographic survey, and a social validity questionnaire. Findings …

Assessing The Social, Economic, And Environmental Impacts Of A Multifunctional Crop: The Case Of Moringa Oleifera, Yoel Gebrai

USF Tampa Graduate Theses and Dissertations

Multifunctional crops have the potential to support societal goals toward more sustainable production of food, feed, and fuel. The production and processing of multifunctional crops, with their numerous uses and coproducts, is accompanied by social, economic, and environmental impacts that need to be systematically evaluated to avoid unintended consequences from widespread adoption. Complexity emerges in the presence of multifunctionality, and counterintuitive system responses can occur. As a result, this dissertation has sought to enhance and apply frameworks to assess multifunctional crop sustainability holistically. Systems thinking and life cycle approaches are applied to a multifunctional crop case study, Moringa oleifera (MO). …

The Discovery Of First Ret Protac With In Vivo Activity, Yafeng Wang

USF Tampa Graduate Theses and Dissertations

This report mainly discusses the development of RET Proteolysis targeting chimera PROTAC molecules based on Selpercatinib (LOXO-292) and Sulfono-γ-AApeptide PROTAC. A PROTAC molecule is a bifunctional molecule composed of two active domains and a linker. It works by inducing selective protein intracellular degradation. This technology was first discussed by Kathleen Sakamoto, Ray Deshaies and Craig Crews in 2001. Using various E3 Ligase, such as CRBN, pVHL, beta-TrCP1, Mdm2, DCAF15, DCAF16, RNF114, and c-IAP1, the PROTAC technology has been applied to several novel drug development,. Yale University licensed the PROTAC technology to Arvinas in 2013–14. For my research, Chapter one summarizes …

The Influence Of Recycled Water On Microbial Source Tracking Techniques That Aim To Identify Untreated Sewage In Surface Waters, Aldo E. Lobos

USF Tampa Graduate Theses and Dissertations

Fecal pollution in recreational waters can introduce pathogens that cause increased human health risk, where exposure can occur during recreational activities such as swimming, diving, surfing, and fishing. Furthermore, human fecal pollution (i.e., sewage) can introduce nutrients and other harmful chemicals that may negatively affect the environment and aquatic species. Generally, sewage is considered the highest risk to human health compared to animal fecal pollution because it typically contains a high diversity of pathogens (including human specific viruses), antibiotic resistant bacteria and genes associated with antibiotic resistance. However, pathogens are difficult to detect due to their low concentrations in the …

An Inference-Centric Approach To Natural Language Processing And Cognitive Modeling, Animesh Nighojkar

USF Tampa Graduate Theses and Dissertations

Reasoning over natural text is highly nuanced, and interpretations can vary widely depending on cultural background, financial status, age, gender, or even mood. This doctoral dissertation seeks to not only mimic human reasoning behaviors but also improve the task used in natural language processing (NLP) to capture naturalistic reasoning, known as the Natural Language Inference (NLI) task. NLI involves determining whether a hypothesis is true (entailment), false (contradiction), or indeterminate (neutral) based on a given premise. Initially, we will investigate the extent to which NLP systems designed to capture semantic equivalence actually measure meaning equivalence. After establishing that they do …

The Role Of L-Fucose In Dendritic Cell Antigen Presentation And Polarization, Chase J. Burton

USF Tampa Graduate Theses and Dissertations

Since the FDA approval of IL-2 in cancer treatment in the 1980s, immunotherapy has emerged as the standard of care for several tumor types (e.g., melanoma, lung, bladder, colorectal). However, approximately 70 percent of cancer patients, independent of prior treatment or staging, administered immunotherapy will not achieve complete responses and will exhibit disease progression. Several factors have been identified to contribute to treatment failure, including the accumulation of intratumoral immunosuppressive cell populations, insufficient tumor-infiltrating anti-tumor immune populations, or the occurrence of immunoediting on tumor cells. Current strategies to circumvent immunosuppression either lack durable response to new immunosuppressive mechanisms the tumor …

The Asymmetric Total Synthesis Of Membranolide And Efforts Towards The Oxeatamides, Sean Bradley

USF Tampa Graduate Theses and Dissertations

Total synthesis is a discipline within organic chemistry that is a constant force that propels the field forward. In our lab, we have immersed ourselves within the field of total synthesis to gain access to complex natural products, perform SAR studies, as well as highlight interesting methodology. Within these endeavors, we have completed the first asymmetric synthesis of membranolide. This has allowed us to explore potential biofilm eradication in our efforts to develop novel therapeutics to combat the ESKAPE pathogens and multi-drug resistance. We have also begun to pursue the first asymmetric total synthesis of the oxeatamides. By utilizing our …

Group B Streptococcus Intestinal Barrier Disruption And The Protective Role Of Butyrate, Kristen Dominguez

USF Tampa Graduate Theses and Dissertations

Group B Streptococcus (Streptococcus agalactiae; GBS) is a leading cause of neonatal sepsis worldwide. Intrapartum antibiotics in GBS-colonized mothers significantly reduced the incidence of early-onset sepsis occurring in the first week of life; however, there are currently no prophylactic interventions for late-onset sepsis, occurring within the first 3 months of life. GBS is a pathobiont that primarily colonizes the gastrointestinal tract and can cross the intestinal barrier via both the paracellular and transcellular routes, causing invasive disease. Neonatal susceptibility to GBS intestinal translocation is mediated by inadequate epithelial barrier function and microbiome immaturity; however, the mechanisms by which GBS exploits …

Modeling Pelagic Feeding In The Gulf Of Mexico With Implications For Ecosystem-Based Fisheries Management, Rebecca L. Scott

USF Tampa Graduate Theses and Dissertations

The focus of this dissertation was simulating trophic dynamics in the pelagic Gulf of Mexico (GOM) with an emphasis on ecosystem-based fishery management (EBFM) strategies for large pelagic species. Fisheries management in the United States largely utilizes single-species assessments, although managers are increasingly interested in EBFM approaches to better account for ecosystem effects on stocks such as predator/prey dynamics. Production in the surface layer of the pelagic zone is variable with environmental conditions and prey is often concentrated in patches by hydrodynamic features. Production is also transported vertically via diel vertical migration, during which deep-sea species provide potentially key forage …

Black Holes In Time: The Effect Of Grid Radiation On The Tumor Immune Microenvironment, Rebecca A. Bekker

USF Tampa Graduate Theses and Dissertations

Tumor-immune interactions shape a developing tumor and its tumor immune microenvironment (TIME) resulting in either well-infiltrated, immunologically inflamed ‘hot’ tumor beds, or ‘cold’ immune deserts with low levels of infiltration. The pre-treatment immune state of the TIME is associated with treatment outcome; immunologically hot tumors generally exhibit better responses to radio- and immunotherapy than cold tumors. However, radiotherapy is known to induce opposing immunological consequences, resulting in both immunostimulatory and inhibitory responses. In fact, it is thought that the radiation induced tumoricidal immune response is curtailed by subsequent applications of radiation. Thus, I hypothesize that spatially fractionated radiotherapy (SFRT), administered …

The Use Of Proactive Progressive Muscle Relaxation To Increase Academic Engagement For Elementary School Students, Lillian G. Mccook

USF Tampa Graduate Theses and Dissertations

Progressive muscle relaxation (PMR) has been shown to produce desired outcomes by decreasing disruptive behavior in the classroom and increasing academic engagement. The integration of technology into the educational setting alleviates some of the burden on educators and enhances the learning experience for students. This study evaluated the effectiveness of brief video-based PMR to increase academic engagement in the classroom with three elementary school students diagnosed with autism spectrum disorder (ASD). Additionally, this study assessed the feasibility and acceptability of video based PMR within a classroom setting. Results from this study show that PMR was a feasible, acceptable and effective …