Digital Commons Network^™

Effects Of Early Acoustic Stimulation On Prepulse Inhibition In Mice, Lisa Tanner

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to determine the effects of an atypical pattern of early acoustic stimulation on auditory development. Previous human research suggests that the acoustic environment of pre-term human infants in the Neonatal Intensive Care Unit (NICU) negatively affects some aspects of auditory development. Animal research suggests that premature auditory stimulation interrupts auditory development. Because mice are born before their auditory systems are developed, they make an excellent model for research on fetal and postnatal plasticity of the auditory system. The premature auditory state of newborn mice is similar to that of the NICU pre-term infant, albeit, …

Frequency Distributions Of Escherichia Coli Subtypes In Various Fecal Sources Over Time And Geographical Space: Application To Bacterial Source Tracking Methods, Matthew A. Anderson

USF Tampa Graduate Theses and Dissertations

Bacterial source tracking (BST) methods often involve the use of phenotypic or genotypic fingerprinting techniques to compare indicator bacteria such as Escherichia coli isolated from unknown sources against a library of fingerprints from indicator bacteria found in the feces of various known source animals. The predictive capability of a library is based in part on how well the library isolates reflect the true population diversity of indicator bacteria that can potentially impact a water body. The purpose of this study was to compare the behavior of E. coli population structures in the feces of humans, beef cattle and horses across …

Studies On Antibacterial Activities Of N-Thiolated Β-Lactams And Their Polymeric Nanoparticles Against Mrsa, Jeung-Yeop Shim

USF Tampa Graduate Theses and Dissertations

Methicillin-resistant Staphylococcus Aureus (MRSA) is now the most challenging bacterial pathogen affecting patients in hospitals and in care centers, and has brought on the need to develop new drugs for MRSA. This thesis centers on studies of N-thiolated β-lactams, a new family of potent antibacterial compounds that selectively inhibit the growth of methicillin-resistant Staphylococcus aureus (MRSA).

Chapter 1 describes MRSA in more detail. Chapter 2 outlines experiments on the effect of a fatty ester group (CO₂R) on the C₄-phenyl ring of N-methylthio β-lactams, expecting that attachment of long chain ester moieties might increase the hydrophobicity, …

Characterization Of Obsidian Sources In Pantelleria, Italy, Barbara A. Vargo

USF Tampa Graduate Theses and Dissertations

The study of prehistoric trade and exchange networks in the Western Mediterranean is directly linked to the identification and location of commodities available to Neolithic communities in that region. One of these commodities is a volcanic glass commonly known as obsidian.

This investigation focused on the procurement, processing, and distribution of obsidian from the island of Pantelleria, situated between the southwestern coast of Sicily in the Straits of Sicily and the northeastern coast of Africa near Cape Bon, Tunisia. Previous studies indicate that there are several chemically different source areas on the island.

Research involved the identification of primary obsidian …

Phylogeography And Evolution Of The Florida Crown Conch (Melongena Corona), Kenneth A. Hayes

USF Tampa Graduate Theses and Dissertations

Melongena corona and closely related congeners are a conspicuous part of the marine intertidal benthic communities of Florida and southeastern Alabama. Significant genetic differentiation among adjacent populations has been conjectured based on variation in shell morphology, habitat discontinuity, low levels of adult motility, and the presence of an aplanic lecithotrophic larval stage. Furthermore, studies of the highly variable shell morphology often have resulted in confusing specific and subspecific definitions of these gastropods, which are often referred to as the "corona complex". Variation in shell morphology may indicate local adaptation or environmentally induced phenotypic plasticity. In this study I utilized mitochondrial …

Characterization And Correlation Analysis Of Pharmaceutical Gelatin, Pascal Georges Felix

USF Tampa Graduate Theses and Dissertations

The properties of the aged gel and subsequent softgels were examined using mechanical and chemical testing methods. Our hypothesis was that a negligible variation will exist between the aged gel of the same type. The greater difference is expected to be seen between the types of gels described as 150 Bloom (alkaline treated collagen) and 195 Bloom (acid treated collagen).

The types of gelatin used were the acid processed (195 Acid Bone) and alkaline processed (150 Lime Bone). Because of the differences expressed as the result of their manufacture sequence (namely their molecular weights), it follows that physical attributes will …

N-Thiolated Β-Lactams: Chemistry And Biology Of A Novel Class Of Antimicrobial Agents For Mrsa, Timothy E. Long

USF Tampa Graduate Theses and Dissertations

N-Methylthio beta-lactams represent a promising new family of antibacterial agents whose in vitro activity is confined largely to Staphylococcus species, including multidrug-resistant forms of S. aureus. Originally developed in the 1980's for use as synthetic intermediates, N-methylthio beta-lactams have recently been shown in these laboratories to possess intriguing biological properties which are addressed in Chapters I-IV. In terms of the antibacterial activities, the structural features and species specificities exhibited by these compounds are unlike those of any existing family of beta-lactam drugs. The lactams seem to exert their effects intracellularly, requiring passage of the bioactive species through the …

Substrate-Based Inhibitors Of Peptidylglycine Á-Amidating Monooxygenase (Pam) As Anti-Proliferative Drugs For Cancer, Geoffrey H. Chew

USF Tampa Graduate Theses and Dissertations

C-Terminal glycine-extended prohormones are enzymatically converted to á-amidated peptides, by peptidylglycine á-amidating monooxygenase (PAM). PAM is a bifunctional enzyme with two catalytic domains: peptidylglycine á-hydroxylating monooxygenase (PHM) and peptidylglycine peptidylglycineaminoglycolate lyase (PAL).

PAM has a significant role in the proliferation of androgen-independent prostate cancer. Thus, the inhibition of PAM could halt cancer growth. Hippurate and hippurate analogs were used as lead compounds for developing inhibitors for PAM. The hippurate analogs exhibiting the highest affinity to PAM (lowest inhibition constant) did inhibit the growth of human androgen-independent prostate cancer DU 145 cells.

Scheduling Of 2-Operation Jobs On A Single Machine To Minimize The Number Of Tardy Jobs, Radhika M. Yeleswarapu

USF Tampa Graduate Theses and Dissertations

This study focuses on the study of a unique but commonly occurring manufacturing problem of scheduling of customized jobs consisting of two operations on a single multi-purpose machine with the performance objective of minimizing the number of tardy jobs (jobs that are not completed by their due dates). Each customized job to be complete needs one unique operation and one common operation performed on it. We considered a static case in this work. The objective of minimizing the number of tardy jobs is considered where all jobs have equal weights and the maximum tardiness has no effect on the performance. …

Comida Sin Frijoles No Es Comida: Evaluation Of A Type 2 Diabetes Education Program For Latinos, Danielle R. O'Connor

USF Tampa Graduate Theses and Dissertations

This thesis describes an internship for the Department of Anthropology that was part of the Florida Health Literacy Study (FHLS) conducted at the University of South Florida College of Public Health, Department of Community and Family Health in the spring and summer of 2003. The FHLS implemented Pfizer Inc.'s For Your Health program, a type 2 diabetes and hypertension education programs, at 14 community health clinics across the state of Florida. The internship was designed to elicit the experiences of 10 bilingual health educators about their experiences and their perceptions of the experiences of their Latino patients with type 2 …

Vibrofluidized Bed Drying Of Citrus Processing Residue For Byproduct Recovery, Eric A. Roe

USF Tampa Graduate Theses and Dissertations

Approximately 44% of the citrus that is processed becomes processing residue. The residue consists of the non-juice components of a citrus fruit, primarily peel and pulp, and is recovered by conversion to animal feed. The material is hygroscopic, agglomerating, has a wide particle size distribution, and must be carefully dried to avoid thermal damage to nutrients and flavors. This dissertation evaluates the possibility of utilizing a vibrofluidized bed dryer for citrus processing residue. Results demonstrate that it is possible to overcome the agglomeration difficulties associated with this material, offering an economically viable alternative processing methodology.

To properly analyze this proposed …

Characterization Of Cadmium Zinc Telluride Solar Cells, Gowri Sivaraman

USF Tampa Graduate Theses and Dissertations

Currently thin film solar cells have efficiencies in the range of 16-18%. Higher efficiencies of 20% or more can be achieved by two junction solar cells in which two p-n junctions are connected in series one on top of the other in a tandem structure. The ideal bandgaps for optimum efficiency in a tandem structure are about 1eV for the top cell and 1.7 eV for the bottom cell. Copper Indium Gallium di-Selenide (CIGS) with a bandgap of 1 eV is a suitable candidate for the bottom cell and Cadmium Zinc Telluride (CZT) with a tunable bandgap of 1.44-2.26 eV …

An Assessment Of Paired Similarities And Card Sorting, Theodore James Dwyer

USF Tampa Graduate Theses and Dissertations

Alcohol expectancies have been shown to be predictive of risk for alcohol problems. Experimental research studies have challenged participants' expectancies with the end result demonstrating a mediational effect on participant drinking. Cognitive research using priming and word recognition tasks have led to the theory that expectancies operate in an associative network. Using dissimilarities information this network has been mapped using multidimensional scaling. The current techniques for collecting dissimilarities information directly in alcohol expectancy research has been limited to the use of the paired comparisons tasks. In order to investigate the utility of a different similarities task a comparison was made …

Development And Testing Of A Haptic Interface To Assist And Improve The Manipulation Functions In Virtual Environments For Persons With Disabilities, Rohit Tammana

USF Tampa Graduate Theses and Dissertations

Robotics in rehabilitation provides considerable opportunities to improve the quality of life for persons with disabilities. Computerized and Virtual Environment (VE) training systems for persons with disabilities, many of which utilize the haptic feedback, have gained increasing acceptance in the recent years. Our methodology here is based on creating virtual environments connected to a haptic interface as an input device. This robotic setup introduces the advantages of the haptic rendering features in the environment and also provides tactile feedback to the patients.

This thesis aims to demonstrate the efficacy of assistance function algorithms in rehabilitation robotics in virtual environments. Assist …

Fishing-Dependent Communities On The Gulf Coast Of Florida: Their Identification, Recent Decline And Present Resilience, Yu, Huang

USF Tampa Graduate Theses and Dissertations

U.S. fisheries legislation requires National Marine Fisheries Service (NMFS) to attend to the critical social and economic issues surrounding the definition and identification of fishing communities, and to the effects that changes to the physical environment and regulatory decisions can have on such communities. To fulfil their mandate, National Marine Fisheries Service (NMFS) sponsored the research entitled "Identifying Fishing Communities in the Gulf of Mexico" to study the economic, social and cultural status of potential fishing communities along the Gulf of Mexico.

NMFS contracted the research project to Impact Assessment, Inc. to study 80 plus potential fishing communities in the …

Transport And Survival Of Water Quality Indicator Microorganisms In The Ground Water Environment Of Florida: Implications For Aquifer Storage And Waste Disposal, David E. John

USF Tampa Graduate Theses and Dissertations

Ground water resources are heavily used for drinking water supply and often as a receptacle for waste water. One concern is the possible contamination of wetland areas by ground water receiving septic system infiltration. To investigate this, two tracer studies were performed using the bacteriophage PRD-1 by seeding septic systems adjacent to wetlands with the phage and monitoring migration towards wetland areas. Transport velocities were evaluated based on appearance of tracer in sampling wells at various distances from the injection point. Velocities were estimated to be 0.25 m/d and 0.4 m/d at the two sites. Some retardation with respect to …

Feasibility And Emissions Of Compression Ignition Engines Fueled With Waste Vegetable Oil, Morgan H. Crawford

USF Tampa Graduate Theses and Dissertations

Research and experience has shown that vegetable oil can be processed, by transesterification, into a useable fuel for compression ignition engines. Earlier research examined using straight vegetable oil as a fuel, but found it to cause detrimental engine problems. Trial and error has shown that heating the vegetable oil prior to injection, is a viable option. A diesel vehicle engine was operated for over 188 hours or approximately 7,000 miles, using waste cooking oil as fuel. The longevity of the vehicle engine was limited by an undetermined engine failure.

Using stationary testing, with no engine load and various power settings, …

Characterization And Formation Of Particulate Nitrate In A Coastal Area, Melissa Cheryl Foster Evans

USF Tampa Graduate Theses and Dissertations

Particulate nitrates play important roles in the atmosphere. They consist mainly of NH₄NO₃ and NaNO₃, products from the reactions of gaseous HNO₃ with gaseous NH₃ and sea salt, respectively. The gas-to-particle phase conversion of nitrate changes its deposition characteristics and ultimately changes the transport and deposition rates of the locally produced species. Studies were conducted to develop background information on the particle concentrations and size distributions and the chemistry and kinetics behind the interactions.

The predominant nitrate species in the Tampa Bay area was identified as coarse mode NaNO₃. NH_{4 …}

Genetic Changes In Natural Populations Caused By The Release Of Cultured Fishes, Michael Dominic Tringali

USF Tampa Graduate Theses and Dissertations

Genetic changes likely occur in wild fish populations as a consequence of interactions with cultured fish, but to what extent do those changes threaten the maintenance of natural genetic diversity and population viability? Following a review and categorization of numerous processes suspected of being agents of post-release genetic change in recipient wild populations (Chapter 1), I focus on risks relating to the magnitude and duration of releases -- but with a twist. That is, I assume that the mean fitness of released, cultured individuals does not differ from that of the recipient natural population. Throughout, attention is devoted to potential …

Perceptions Of Family Functioning Between Children With Behavior Difficulties And Their Primary Caregiver, Melissa Farino Todd

USF Tampa Graduate Theses and Dissertations

This research study compared perceptions of family functioning among preadolescent children with behavior difficulties and their primary caregivers. Participants consisted of 29 caregiver-child dyads as well as each child's classroom teacher. Eligibility for the study was based on the child's placement within a self-contained Emotionally Handicapped (EH) or Severely Emotionally Disturbed (SED) classroom in one of three elementary schools within two west coast Florida counties. Data collection included teacher rating scales pertaining to the severity of each child's behavior and the presence of Callus Unemotional (CU) traits in addition to caregiver and child interviews tapping perceptions of family functioning.

Results …

An Archaeological Study Of Architectural Form And Function At Indian Key, Florida, Kelly A. Driscoll

USF Tampa Graduate Theses and Dissertations

Indian Key Historic State Park is a small island located on the Atlantic Ocean side of the Florida Keys, near Islamorada. Before it was bought by the state of Florida in 1970, Indian Key had been the setting for a number of historically significant activities. The most well known of these is the 1840 raid on the people and buildings that made up a small wrecking village, established on the island by Jacob Housman in the early 1830s. The limestone foundations of these structures are the main attraction to today's visitor to the park.

There is more to the story …

Patterns Of 4th Graders' Literacy Events In Web Page Development, Rewa Colette Williams

USF Tampa Graduate Theses and Dissertations

This study describes in-class and home literacy events that occur when students work in groups to create web pages as evidence of learning the academic content that was presented within a fourth grade classroom. The constructivist approach to learning was the underpinning idea examined as well as its connection to technology and group work. Data were collected in a variety of ways to obtain a picture, as comprehensive as possible, of the oral, listening, viewing, and written on-task communication and interactions that occurred. As the in-class and home literacy events emerged, the competencies and strategies that students used while interacting …

Historical Archaeology Of The Indian Key (8mo15) Warehouse: An Analysis Of Nineteenth-Century Ceramics, Lisa Nicole Lamb

USF Tampa Graduate Theses and Dissertations

This thesis describes the archaeological investigation of the Warehouse Complex on Indian Key (8MO15), Monroe County, Florida, through the study of the ceramics recovered from excavations conducted there by the State of Florida from 1972 to 1973 and by the University of South Florida from 1998 to 2002. The Warehouse Complex is composed of two distinct architectural areas, referred to as Feature A and Feature C. This complex lies on the north shore of Indian Key, located in the Atlantic Ocean in the Middle Keys near Islamorada, Florida. The town of Indian Key was founded in the early 1820s, and …

Your Computer Is Watching You: Intelligent Agents And Social Facilitation, Jason R. Read

USF Tampa Graduate Theses and Dissertations

This study investigates whether the social facilitation effect takes place when a person performs a computerized task that includes an animated intelligent agent (IA). The moderating effects of two individual differences, locus of control (LOC) and microcomputer playfulness (MCP), are tested for. It was proposed that an IA's presence would cause participants to exhibit this effect and that LOC and MCP would moderate a participant's arousal, measured as state anxiety, such that those possessing an internal LOC and those exhibiting high MCP would experience less arousal when performing computerized tasks with an IA present. Data was analyzed using a 2 …

Use Of Silica-Supported Adsorbents, Modified Silica Gel, Modified Montmorillonite Ksf And Magnetite In The Remediation Of Selected Heavy Metals From Aqueous Media, Craig Alcindor Ivan Bowe

USF Tampa Graduate Theses and Dissertations

Pollution of the earth's water resources, including freshwater sources such as lakes and rivers, by heavy metals have been a detriment to the environment for as long as the industrial age of man. As these metallic pollutants accumulate in the supply of precious groundwater and drinking water the need for clean-up technologies to combat the increasing threat is becoming of more importance to government and industry alike. Previous work has established the possibility of using known chelating agents, such as LIX-54® supported on silica gel in the removal of selected heavy metal ions such as copper(II), nickel(II) , and cadmium(II) …

Organizational Office Space In The Virtual Age: The Role Of Shared Space In Communication, Sheila Gobes-Ryan

USF Tampa Graduate Theses and Dissertations

This thesis takes a phenomenological approach to the examination of the organizational need for shared office space. Questions are developed in a reflexive narrative that introduces challenges to the assumption that space is a given in organizations. The narrative also uses the process of questioning this basis assumption to develop a new understanding of the role of space in organizations in supporting the development of common language needed for the creation of organizational knowledge. Key ideas from systems theory, autopoiesis, organizational theory, semiotics, and psychology are utilized as resources developing the ideas.

A Molecular Approach To Assessing Meiofauna Diversity In Marine Sediments, Heather C. Hamilton

USF Tampa Graduate Theses and Dissertations

A Molecular Approach to Assessing Meiofauna Diversity in Marine Sediments Heather C. Hamilton Abstract The purpose of this study was to determine if a molecular approach could be applied to calculating the diversity of meiofauna in marine sediments from two sites in Tampa Bay, FL, similar to the approach of McCaig et al, 1999 in calculating the diversity of microbes in pastureland soils. The approach includes extracting total DNA directly from the sediment and amplifying the 18S rRNA gene by PCR. Clone libraries from the 18S gene would be created for each site and 300 sequences from each clone library …

Dermal Absorption Of A Dilute Aqueous Solution Of Malathion, John E. Scharf Md

USF Tampa Graduate Theses and Dissertations

Malathion is a commonly used organophosphate pesticide on field crops, fruits, nut trees, vegetables, livestock, agricultural premises, and land. The approved uses also include mosquito and medfly control. These uses can result in human skin contact. The purpose of this study is to evaluate the human skin absorption of malathion for the purpose of assessing the risks associated with aqueous solution exposures following applications. Aerial applications can result in solubilized malathion in swimming pools and other waters that may be contacted. Human volunteers were selected and exposed to aqueous solutions of malathion at various concentrations. Participants submerged their arms and …

Reproductive Characteristics, Multiple Paternity And Mating System In A Central Florida Population Of The Gopher Tortoise, Gopherus Polyphemus, Jamie Colleen Colson-Moon

USF Tampa Graduate Theses and Dissertations

I studied the reproductive characteristics and mating systems of a central Florida population of gopher tortoises (Gopherus polyphemus). Using x-radiography, females were monitored for stage in egg-shelling and clutch size. Eggs began to appear on x-ray photographs in the first week of May in both 2001 and 2002; however, fully shelled eggs were not found before the end of May. In total 55% of the females x-rayed were gravid. Clutch sizes ranged from 4-12 with a mean of 7.29, with a mean clutch mass of 40.9 g. Clutch size increased with an increase in mean carapace length and …

Direct 3d Interaction Using A 2d Locator Device, Anees Ansari

USF Tampa Graduate Theses and Dissertations

Traditionally direct 3D interaction has always been limited to true 3D devices whereas 2D devices have always been used to achieve indirect 3D interaction. Till date no proper research has been done to try and extend the use of mouse to direct 3D interaction. In this research we explore the issues involved with using the mouse to accommodate the additional degrees of freedom required for 3D interaction. We put forth a unique and innovative design to achieve this objective and show that even a device as simple as the mouse can be highly effective for 3D interaction when supported by …