Digital Commons Network^™

Negotiating Artistic Identity Through Satire: Subreal 1989-1999, Anca Izabel Galliera

USF Tampa Graduate Theses and Dissertations

The focus of this research study is on major art works produced during the nineteen-nineties by the Romanian collective subREAL, composed of Calin Dan and Josif Kiraly. The thesis is an alternative to the literal-minded and politically biased Western view typified in two major exhibitions of art from Eastern Europe: Beyond Belief: Contemporary Art from East Central Europe (Chicago, 1995) and After the Wall: Art and Culture in Post-Communist Europe (Sweden, 1999). Both exhibitions presented Post-Communist nations as a monolithic bloc, in which art was primarily a passive reflection of political and social events. It will be demonstrated that …

The Influence Of Pre-Existing Memories On Retrieval-Induced Forgetting, Leilani B. Goodmon

USF Tampa Graduate Theses and Dissertations

When people form episodic connections between memories that share a common retrieval cue, the tendency for those memories to interfere in later retrieval is often eliminated, and forgetting of the interfering information is reduced. For example, episodic integration protects memories from retrieval-induced forgetting (RIF), a phenomenon in which practicing retrieving some associates of a cue leads to the suppression of others that interfere with retrieval (Anderson, Green, & McCulloch, 2000). The purpose of this study was to determine whether semantic integration, as a result of pre-existing associations between practiced items and their unpracticed competitors, also moderates RIF. This research was …

The Role And Regulation Of Argininosuccinate Synthase In Endothelial Function, Bonnie L. Goodwin

USF Tampa Graduate Theses and Dissertations

While cellular levels of arginine greatly exceed the apparent Km for endothelial nitric oxide synthase (eNOS), nitric oxide (NO) production is limited by availability of arginine. Results from this work have provided a unique understanding of endothelial NO production, showing that arginine regeneration, that is the recycling of citrulline back to arginine by argininosuccinate synthase (AS) and argininosuccinate lyase (AL), defines the essential source of arginine for NO production. Using RNA interference analysis, selective reduction of AS expression was shown to directly correspond with a diminished capacity of endothelial cells to produce NO, despite saturating levels of arginine in the …

Attenuation Of Bromobenzene-Induced Hepatotoxicity By Poly(Adp-Ribose) Polymerase Inhibitors, Kelly Waggoner Hall

USF Tampa Graduate Theses and Dissertations

Previous studies have shown extensive cellular damage can activate poly(ADP-ribose) polymerase-1 (PARP-1) and cause a rapid decrease in the levels of NAD+ and ATP, thereby preventing apoptosis and promoting necrosis and inflammation. The purpose of this study was to extend previous observations that inhibitors of PARP-1 could alter acetaminophen and carbon tetrachloride-induced hepatotoxicity. Bromobenzene (BB) a glutathione dependent hepatotoxicant was tested. Groups of male mice were treated with a single dosage of 112mg/kg (0.075 ml/kg) BB by the intraperitoneal (ip) route. All animals were maintained in a controlled environment and provided food and water ad libitum. This dosage of BB …

Prehistoric Human Subsistence Patterns In Northern Patagonia, Argentina: Isotopic Evidence For Reconstructing Diet, Scott Grammer

USF Tampa Graduate Theses and Dissertations

This study investigates the isotopic signatures of human skeletal remains that were recovered from several sites along the coast and inland in the north-central Patagonian region of Argentina. Human skeletal remains, dating from 2500 BP through the early historic period, are examined to determine the relative significance of terrestrial and aquatic food resources and subsequently, the extent to which coastal food resources were exploited by indigenous Argentinians. Carbon and nitrogen isotopes contained within human bone collagen and apatite are measured quantitatively to determine the relative significance of marine and terrestrial foods. This study, one of the first isotopic studies of …

Development Of A Dna Vaccine Against Streptococcus Mutans: A Novel Approach To Immunization Against Dental Caries, Thomas Han

USF Tampa Graduate Theses and Dissertations

Streptococcus mutans is the main causative agent of dental caries, which is a widespread infectious disease. A number of surface molecules are involved in the pathogenicity of this organism, including adherence and aggregation factors. The wallassociated protein A (WapA) of Streptococcus mutans GS-5 was previously demonstrated to be a sucrose-dependent adherence and aggregation factor, and is a larger precursor to extracellular antigen A (AgA), a candidate antigen for a dental caries vaccine.

The full-length wapA gene and a C-terminal truncated version agA encoding the AgA were cloned into the mammalian expression vector pcDNA 3.1/V5/His-TOPO. The above constructs were …

Standardizing The Auditory Evoked Potential Technique: Ground-Truthing Against Behavioral Conditioning In The Goldfish, Carassius Auratus, Randy J. Hill

USF Tampa Graduate Theses and Dissertations

Auditory evoked potentials (AEPs) have become commonly used to measure hearing thresholds in fish. However, it is uncertain how well AEP thresholds match behavioral hearing thresholds and what effect variability in electrode placement and tank composition has on AEPs. In the first experiment, the effect of testing tank composition and electrode placement on AEPs was determined by recording AEPs in the same individual fish in a steel and PVC cylindrical testing tank, and simultaneously recording AEPs from four locations and two different depths on each of 12 goldfish, Carassius auratus. Results from these studies show that tank composition has …

The Position Of The Ophiuroidea Within The Phylum Echinodermata, Mary C. Harmon

USF Tampa Graduate Theses and Dissertations

Cladistic analyses of the interclass relationships of the phylum Echinodermata have not provided a phylogeny that is separately supported by both larval and adult characters. Similar to the reported incongruence with cladistic analyses, molecular analyses of ribosomal RNA (rRNA) genes have also given ambiguous results, which could be due to a number of factors. The use of short sequences, systematic errors such as long branch attraction, and mis-alignments of the data that are introduced by programs which are unsuitable for non-protein coding genes, have resulted in a controversy as to the true nature of echinoderm relationships. Historically, it is the …

Hydrogen Fluoride Method Development For The Ogawatm Passive Sampling Device, Ilsa Johansson

USF Tampa Graduate Theses and Dissertations

This study tested the precision and accuracy of a triethanolamine (TEA) absorbent in the OgawaTM Passive Sampling Device (PSD) for detection of ambient hydrogen fluoride (HF). The project was initiated to develop a method to verify compliance with emissions regulations for Coronet. Field and laboratory trials were conducted.

Mixed cellulose ester filters were saturated with 70% TEA and placed in the PSDs. Aermod ISCT3 modeled ambient HF concentrations at Coronet to guide deployment of PSDs at 28 sampling stations, 3 PSDs per station, 500 – 3500 meters from Coronet. After 30 days of sampling, ambient HF concentrations were calculated from …

A Comparison Of Traditional Physical Laboratory And Computer Simulated Laboratory Experiences In Relation To Engineering Undergraduate Students’ Conceptual Understandings Of A Communication Systems Topic, Giti Javidi

USF Tampa Graduate Theses and Dissertations

This study was designed to investigate an alternative to the use of traditional physical laboratory activities in a communication systems course. Specifically, this study examined whether as an alternative, computer simulation is as effective as physical laboratory activities in teaching college-level electronics engineering education students about the concepts of signal transmission, modulation and demodulation. Eighty undergraduate engineering students participated in the study, which was conducted at a southeastern four-year university.

The students were randomly assigned to two groups. The groups were compared on understanding the concepts, remembering the concepts, completion time of the lab experiments and perception toward the laboratory …

Transforming Public Spaces Through Performance, Anthony G. Valentine

USF Tampa Graduate Theses and Dissertations

This thesis is study about how public spaces can be transformed through performance within them.

More specifically, this study involves two public venues: The Museum of Science and Industry (MOSI), Tampa, FL and The University of South Florida (USF), Tampa, FL in that within these public venues, not only do performances take place, but they transform the space they are performed in and the spaces in-between the public spaces into performance spaces.

A Future With Hope: The Social Construction Of Hope, Help, And Dialogic Reconciliation In A Community Children's Mental Health System Of Care, Christine S. Davis

USF Tampa Graduate Theses and Dissertations

This research examines the social construction of hope in a community mental health system of care. Groopman (2004) defines hope as the elevating feeling we experience when we see a path to a better future. A year-long ethnographic study of a children's mental health system of care team found that members of the mental health care team construct hope for themselves and for the family they're helping by cycling through the dialectical tensions of hegemony and equality, marginalization and normalization, relating and othering, empowerment and disempowerment, and control and emotionality. They reconcile these tensions in dialogic moments of empathy toward …

Simulating The Electric Field Mediated Motion Of Ions And Molecules In Diverse Matrices, Joseph Hickey

USF Tampa Graduate Theses and Dissertations

Electroporation is a methodology for the introduction of drugs and genes into cells. This technique works by reducing the exclusionary nature of the cell membrane [125, 129, 186, 189]. Electroporation has successfully been used in electrochemotherapy and electrogenetherapy [57, 68, 86, 87, 110, 112, 131]. The two major components of electroporation are an induced transmembrane potential and the motion of the deliverable through a compromised cell membrane into the target cell [38, 55, 62, 114, 131]. These two components are both dependent on the electrophoretic motion of charged species in an applied electric field [45, 64, 75, 77, 177].

Currently, …

Neuroinflammation In Alzheimer’S Disease: Characterization And Modification Of The Response Of Transgenic Mice To Intrahippocampal Lipopolysaccharide Administration, Donna Lorraine Herber

USF Tampa Graduate Theses and Dissertations

Alzheimers disease (AD) is pathologically characterized by amyloid plaques, neurofibrillary tangles, inflammation, and neurodegeneration. According to the amyloid hypothesis of AD, the central mediating event of the disease is the deposition of amyloid. The inflammation hypothesis of AD states that it is the inflammatory response to plaques and tangles, rather than the actual lesions, which causes the disease. Studies described here combine the two approaches into a single model. Four studies are presented using a basic protocol of intrahippocampal lipopolysaccharide (LPS) injection to stimulate inflammation in transgenic mice. The first study looked at alpha7 nicotinic receptors during the glial response …

Evaluation Of The Current State Of Florida West Nile Surveillance Program As A Predictor For Control And Prevention Of Human West Nile Diseases, Angela E. Butler

USF Tampa Graduate Theses and Dissertations

West Nile is an important novel virus in the United States, having spread rapidly since it was first detected in New York in 1999. The Centers for Disease Control and Prevention as well as many State Health Departments, have mandated programs for surveillance of West Nile Virus activity. These programs incorporate many different aspects including existing arboserology programs with additional testing for West Nile Virus and new plans that incorporate active and passive surveillance methods.

The objective of this study was to examine all aspects of the Florida West Nile surveillance program to determine if there was transmission in the …

Investigating Second Seminole War Sites In Florida: Identification Through Limited Testing, Christine Bell

USF Tampa Graduate Theses and Dissertations

This thesis uses the results of limited testing at the Fort Dade (1836-1842) and Hope Homestead (1842-ca. 1900) archaeological sites to establish a method for quickly identifying threatened sites with minimal disturbance to surrounding areas. Artifact analysis, pattern recognition, and comparison with similar known sites are key elements. Pedestrian survey, metal detection, posthole and shovel testing, and test excavation are tools used to accomplish this. Artifact analysis is used to establish date ranges for the sites, as well as the material variation between military and homestead occupations. Artifacts used for analysis include glass, ceramics, nails, arms and personal items. Quantitative …

An Analysis Of Key Deer Herbivory On Forest Communities In The Lower Florida Keys, Mark Allan Barrett

USF Tampa Graduate Theses and Dissertations

The Key deer (Odocoileus virginianus clavium) population has increased from less than 80 individuals in the 1950s to approximately 700 by 2000. Over two-thirds of the Key deer population resides on two islands out of a potential 26 islands within their range. The skewed distribution resulted in high deer densities on Big Pine, No Name, and Big Munson keys. The objective of the study was to evaluate the effects of Key deer herbivory in mangrove, buttonwood, hammock and pineland habitats between islands with low, medium, and high densities of deer. Host-plant choice trials were conducted to determine Key …

Globalization, Ecotourism, And Development In The Monte Verde Zone, Costa Rica, Edgar Allan Amador

USF Tampa Graduate Theses and Dissertations

Ecotourism has been promoted globally as a model for sustainable development because it simultaneously benefits the environment and the residents of the given destination. However, many conservationists have questioned the long term sustainability of ecotourism as it is difficult to mitigate the impact of even low levels of tourism on a particular ecosystem. Further, social scientists including anthropologists have similarly questioned whether most residents of a particular destination actually benefit significantly from the alternative tourism economy.

The Globalization Research Center in cooperation with the Monteverde Institue in the Monte Verde Zone, Costa Rica, is undertaking a longitudinal study -- dubbed …

Lily’S Dilemma: Opposing Principles In The House Of Mirth, Debbie Lelekis

USF Tampa Graduate Theses and Dissertations

The focus of this study is Lily Bart and how she maneuvers in the cold, competitive world of upper class New York. To create a framework for my investigation, I draw upon naturalistic readings of the story which portray Lily as an outsider or "other" in her society. Lily's ethical principles lead to her destruction. Her marriage problem is just an example of her rejection of the life that her society expects her to lead. As she becomes more aware of a different philosophy of life--characterized by Selden's "republic of the spirit"--she finds it impossible to abide by the rules …

Building A Case For The Unfamiliar Cause In Cause-Related Marketing: The Importance Of Cause Vested Interest, Charles G. O'Brien

USF Tampa Graduate Theses and Dissertations

Marketing and advertising practitioners are currently matching up a brand with a cause and broadcasting the association to consumers in a practice called cause related marketing (CRM). Scholars are building a stream of academic research which seeks to understand the relationship between a brand and a cause (a.k.a., alliance) in relation to the final outcome of a CRM campaign. Ostensibly, both partners benefit from this alliance, although many CRM studies seek to understand how to optimize this relationship for each partner.

In professional practice and academic research both practitioners and researchers have focused on established, popular, well-known causes in consideration …

Chemical And Photolytic Degradation Of Polyacrylamides Used In Potable Water Treatment, Peiyao Cheng

USF Tampa Graduate Theses and Dissertations

Polyacrylamides (PAMs) are a class of polymers formed from acrylamide alone or copolymerized with other monomers. PAMs have been used in drinking water treatment as flocculants or coagulants in the highest volume among all the polymer types. In potable water treatment processes, polyacrylamides are often exposed to oxidants (e.g. chlorine and permanganate) and UV irradiation from sunlight or artificial sources. The purpose of this study is to evaluate the possible degradation of PAMs caused by chemical oxidation and/or UV irradiation.

Three types of PAM products (nonionic, cationic and anionic) were studied under free chlorine and permanganate oxidation, UV irradiation, and …

N-Thiolated Β-Lactams: Chemistry, Sar And Intracellular Target Of A Novel Class Of Antimicrobial And Anticancer Agents, Bart Allan Heldreth

USF Tampa Graduate Theses and Dissertations

N-Thiolated β-lactams (1) represent a promising new group of compounds with potent inhibition effects on bacteria, like Bacillus anthracis and methicillin resistant Staphylococcus aureus, and onco-systems, like breast cancer and leukemia. Originally developed as part of a synthetic pathway to bicyclic lactams, N-thiolated β-lactams have been shown in this laboratory to possess intriguing biological activities. The antibacterial activities of this new class of agents rely on novel structural features unlike those of any existing family of β-lactam drugs. The lactams seem to exert their effects intracellularly, requiring passage of the bioactive species through the cellular …

Examining The Current U.S. Beef Trade Policies Concerning The Testing For Mad Cow Disease, David C. Miller

USF Tampa Graduate Theses and Dissertations

Despite existing mad cow disease surveillance efforts in the United States, in place since the 1980s, a cow that tested positive for mad cow disease was granted entrance into the U.S. in December, 2003. The cow that tested positive, according to witnesses, displayed no symptoms that are synonymous with advanced bovine spongiform encephalopathy, BSE. This occurrence had detrimental effects on the U.S. beef export market, as many countries banned American beef. Estimates of the damage inflicted reach into the billions of dollars. BSE in the U.S. has the potential of causing damages in other aspects as well. Aside from the …

Examining The Handbooks On Environmental Journalism: A Qualitative Document Analysis And Response To The Literature, Lisa Rademakers

USF Tampa Graduate Theses and Dissertations

This thesis addressed the question, "How should journalists cover the environment, according to the conversation between the scholarship on environmental journalism and the handbooks on environmental journalism?" Do the handbooks, written for practicing journalists, agree with the academic scholarship on environmental journalism?

The conversation between the literature and handbooks is important to examine, as the handbooks are tools journalists may use when reporting on the environment. The handbooks could influence a journalist, who influences the public, who make decisions in a democracy. As well, examining the conversation between the literature and the handbooks reveals whether or not the academy and …

Microglia Activation In Alzheimer's Disease, Kirk Phillip Townsend

USF Tampa Graduate Theses and Dissertations

The work detailed in this dissertation has an overarching theme of modulating microglia activation in both in vivo and in vitro models relevant to AD. The premise is that understanding microglia function in this context may lead to a better understanding of AD pathogenesis and thus to effective therapeutic interventions. In chapter 3, we employ a well-defined model of microglia activation whereby the intraperitoneal delivery of LPS results in CNS microglia activation and TNF-α production. Having previously identified that CD45 signaling pathways antagonized microglia TNF-α production in vitro and given that immunotherapy with anti-CD45 antibodies are already in clinical trials …

Isolation And Identification Of O-Linked-Β-N-Acetylglucosamine Modified Proteins (O-Glcnac) In The Developing Xenopus Laevis Oocyte, Sreelatha Paspuleti

USF Tampa Graduate Theses and Dissertations

Oocyte development in Xenopus laevis spans six morphologically distinct stages (stage I-VI), and is associated with a decrease in protein O-GlcNAc levels. As a first step in elucidating the role of O-GlcNAc in developing oocytes, initial efforts were focused on isolation and identification of fifteen modified proteins that decrease during oocyte development. Stage I oocytes due to their high amounts of these proteins, were used as starting material for purification. Multiple affinity and specific antibody based purification technique were initially used in an attempt to enrich the O-GlcNAc proteins. Due to the unique properties of the proteins …

Imitation And Its Reciprocity In The Treatment Of Autism, Roxana I. Nedelcu

USF Tampa Graduate Theses and Dissertations

This study examined, within a multiple baseline design, the development of generalized imitative repertoires and the occurrence of contingency testing in children with autism. Generalized imitation refers to an imitative response class maintained by a conditioned reinforcer: similarity. In the case of imitation, a response class is established by reinforcement of sufficient exemplars of different imitations; the results is a general repertoire of imitating novel responses on their first presentation. Generalized imitation was facilitated through reinforcement procedures involving shaping and fading. Multiple experimenters and multiple settings have been involved to promote the generalization of imitation. Contingency testing, which has been …

Using Multiwavelength Uv-Visible Spectroscopy For The Characterization Of Red Blood Cells: An Investigation Of Hypochromism, Akihisa Nonoyama

USF Tampa Graduate Theses and Dissertations

Particle analysis using multiwavelength UV-visible spectroscopy provides the potential for extracting quantitative red blood cell information, such as hemoglobin concentration, cell size, and cell count. However, if there is a significant presence of hypochromism as a result of the concentrated hemoglobin (physiological value of 33%), successful quantification of red cell values would require a correction.

Hypochromism has been traditionally defined as a decrease in absorption relative to the values expected from the Beer-Lambert Law due to electronic interactions of chromophores residing in close proximity of one another. This phenomenon has been suggested to be present in macroscopic systems composed of …

Protecting Biscayne: An Analysis Of Strategies For The Protection Of Biscayne National Park, Janell M. Harvey

USF Tampa Graduate Theses and Dissertations

Biscayne National Park is located off the southeast coast of Florida and attracts approximately half a million visitors annually. Managers of Biscayne National Park are proposing a new General Management Plan (GMP) in order to update the recreational and commercial use of resources in the park. A Fishery Management Plan (FMP) is also being drafted simultaneously in conjunction with the Florida Fish and Wildlife Conservation Commission in order to address concerns associated with management of fish stocks within the park.

The proposed plan alternatives of the GMP and the recommendations of the FMP were developed in response to the negative …

Effects Of Participant Engagement On Alcohol Expectancies And Drinking Outcomes For A Computerized Expectancy Challenge Intervention, William Michael Hunt

USF Tampa Graduate Theses and Dissertations

The purpose of the present study was to examine the effect of varying the amount of participant engagement on alcohol expectancy and drinking outcomes during a social/sexual expectancy challenge based on Darkes and Goldman's (1993, 1998) protocol. This study was also intended to provide a test of the efficacy of administering an alcohol/placebo expectancy challenge outside of a live drinking scenario through video presented as part of a computerized intervention.

One hundred fifty-eight male participants across three sites were randomized into a no-intervention control group that received non alcohol-related information in a minimally interactive computerized format, a low-level engagement experimental …