Digital Commons Network^™

Novel Enzyme Perspectives: Arylalkylamine N-Acyltransferases From Bombyx Mori & 1-Deoxy- D-Xylulose-5-Phosphate Synthase From Plasmodium Falciparum And Plasmodium Vivax, Matthew R. Battistini

USF Tampa Graduate Theses and Dissertations

This dissertation is dedicated to the research and investigation of novel enzymes and the methods used to study them, with physiological roles ranging from isoprenoid biosynthesis to neurotransmitter production. Using a combination of bioinformatics, recombinant cloning, enzymology, and proteomics, we have contributed to the understanding and exploration of several human illnesses, including malaria, cancer, and endocrine dysfunction.

Our first project involved studying the enzymes responsible for N-acylarylalkylamide biosynthesis in Bombyx mori. Very little is known how these potent signaling molecules are produced in vivo, however, one possible pathway is the direct conjugation of an acyl-CoA to a corresponding …

An Animal Model Of Flashbulb Memory: Insights Into The Time-Dependent Mechanisms Of Memory Enhancement, Laura Ashley Bullard

USF Tampa Graduate Theses and Dissertations

The vivid memory of an emotional event, as well as memory for incidental details associated with the arousing event, has been referred to collectively as a “flashbulb memory”. An important aspect of flashbulb memory in people is that an emotional event enhances memory of contextual details, such as the weather, or clothes one was wearing at the time of the event. Therefore, an emotional event not only produces a detailed memory of the event, itself, but also enhances memory for contextual details that would otherwise not be remembered. The first goal of this work is to describe the development of …

Modeling Alphabet Skills As Instructive Feedback Within A Phonological Awareness Curriculum, Arnold Olszewski

USF Tampa Graduate Theses and Dissertations

This study investigated the use of instructive feedback for modeling early literacy skills. Instructive feedback is defined as the presentation of additional information during the positive feedback phase of learner trials. Thus, it is a way of modeling additional information when students respond to a trial correctly. Previous studies have demonstrated that instructive feedback can facilitate students’ observational learning of skills, such as sight words, numerals, and vocabulary. Instructive feedback has yet to be investigated when modeling early literacy skills.

A modified version of an evidence-based early literacy intervention, PAth to Literacy, was used. Studies examining the efficacy of …

Using Behavioral Skills Training And A Warning Sticker To Teach Children Household Poison Safety Skills, Jackalynne Jean Delong

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to assess if Behavioral Skills Training (BST) can be used to train a sticker to function as a discriminative stimulus (Sᴰ) for engaging in household poison safety skills and assess whether this skill generalized to untrained household chemicals that bear the Sᴰ in the form of a sticker. Three typically developing children ages 3 and 5 and their parents participated in this study which took place in their homes. BST effectively taught children to engage in household poison safety skills when they come into contact with the trained household poison(s) labeled with the sticker …

Comparison Of Otolith-Based Growth Rates And Microchemistry In Red Drum Before, During, And After The Deepwater Horizon Oil Spill, Brock Charles Houston

USF Tampa Graduate Theses and Dissertations

Oil from the Deepwater Horizon blowout reached the Gulf of Mexico coast in the summer of 2010 and potentially exposed species living in those areas to toxic chemicals. The purpose of this study is to examine otoliths from Red Drum (Sciaenops ocellatus) for evidence of oil exposure that could be related to reduced growth rates. Because otolith growth and somatic growth are directly related, differences in annulus measurements can indicate differences in annual somatic growth, which is a good indicator of overall fish condition, and translates into changes in survival and lifetime reproductive potential. This study assessed variation …

Understanding Climate Change And Sea Level: A Case Study Of Middle School Student Comprehension And An Evaluation Of Tide Gauges Off The Panama Canal In The Pacific Ocean And Caribbean Sea, Juan Carlos Millan-Otoya

USF Tampa Graduate Theses and Dissertations

The present study had two main objectives. The first was to determine the degree of understanding of climate change, sea level and sea level rise among middle school students. Combining open-ended questions with likert-scaled questions, we identified student conceptions on these topics in 86 students from 7^th and 8^th grades during 2012 and 2013 before and after implementing a Curriculum Unit (CU). Additional information was obtained by adding drawings to the open-ended questions during the second year to gauge how student conceptions varied from a verbal and a visual perspective. Misconceptions were identified both pre- and post-CU among …

He_Rtland: The Violence Of Neoliberalism, Hector Sotomayor

USF Tampa Graduate Theses and Dissertations

Perhaps, under the consciousness of today, “neoliberalism” has defined our world during the previous and current centuries more than any other socioeconomic system. But the evolution of this ideology, which initially aimed to enhance, or rather, reinvent capitalism and individual freedom, has, in essence, induced an unrecognized problem. I argue that neoliberalism is the catalyst for much of the hostility in this globalized society where tensions and poverty are casualties of individual and corporate prosperity. Because of this revelation, I argue that neoliberalism inadvertently instills violence that is both unseen and gendered. In order to formulate my argument, I introduce …

North American River Otter (Lontra Canadensis) Presence And Habitat Analysis In Florida As Compared To Historical Data, Samantha Wilber

USF Tampa Graduate Theses and Dissertations

North American river otters are considered common in the state of Florida, but their populations have not been studied since the 1980’s. Since that time, Florida’s human population has more than doubled, and many natural areas of Florida have been developed. The aim of this study was to determine the presence or absence of river otters at locations in Florida which they historically inhabited. Forty-six sample sites where otters were historically found were obtained from the Florida Museum of Natural History Mammals Master Database (FMNH MMD). These sites were condensed to two focus areas, in and around Alachua and Collier …

The Development Of A Fluorescence-Based Reverse Flow Injection Analysis (Rfia) Method For Quantifying Ammonium At Nanomolar Concentrations In Oligotrophic Seawater, William Abbott

USF Tampa Graduate Theses and Dissertations

The goal of this thesis was to adopt a reverse flow injection analysis (rFIA) technique to the fluorometric analysis of the reaction o-phthaldialdehyde (OPA) with ammonium, allowing accurate measurements of ammonium concentrations lower than the detection limit of the widely used indophenol blue (IPB) colorimetric method while accounting for the background fluorescence of seawater. Ammonium is considered an essential nutrient for primary productivity, especially in the nutrient depleted surface ocean where as the most reduced form of dissolved inorganic nitrogen, it is readily assimilated via metabolic pathways. Challenges in the quantification of ammonium require more sensitive analytical techniques for …

School-Based Concussion Management: Implementation Characteristics Of Student Services Personnel, Jeffrey Garofano

USF Tampa Graduate Theses and Dissertations

Concussion is an injury that disproportionately affects children and adolescents and has the potential to negatively impact school performance. Currently, a significant proportion of youth with concussion go unreported due to a lack of effective concussion surveillance procedures. Additionally, many individuals who have daily contact with youth (e.g., parents, teachers) lack training in how to assess or manage a concussion. Schools may be the ideal setting to address both of these problems as they have a high level of access to students and employ personnel (e.g., school psychologists, nurses, social workers, counselors) with experience in assessment and intervention. What is …

The Effect Of An Alcohol Cue On A Risk Taking Task, Patrick Mcgee Logan

USF Tampa Graduate Theses and Dissertations

Laboratory-based tasks of impulsivity and related constructs can be useful in predicting alcohol use. Performance on these tasks is generally assumed to reflect traits that remain stable across situations. However, several studies have indicated that manipulations of state-like variables (e.g., mood or stress) can influence levels of impulsivity demonstrated on the tasks. Furthermore, environmental context (in the form of physical setting, or contextual cues) has a demonstrable effect on tasks relevant to alcohol-related risky behaviors (e.g., ad lib drinking tasks). Importantly, this effect of context on behavior is dependent on the individual's alcohol expectancies. It is unknown, however, whether alcohol-related …

At The Intersection Of Human Agency And Technology: Genetically Modified Organisms, James Libengood

USF Tampa Graduate Theses and Dissertations

Since the Neolithic period and the rise of agriculture along Mesopotamia’s “Fertile Crescent,” greater societies have formed thus requiring laws and governance to ensure their continued preservation. The Babylonian Code of Hammurabi is one such example of how agricultural technologies directly created new social and institutional structures in codifying slavery into law, or how mercantile transactions are to be conducted. Similarly, GMOs are the result of modern agricultural technologies that are altering laws and society as a result of their implementation. This transformation informs the central inquiries of my research question: Why are GMOs necessary, and what influences do they …

Evaluating Preference For And Effectiveness Of Telehealth And In-Person Parent Training, Jennifer Michelle Stich

USF Tampa Graduate Theses and Dissertations

Telehealth provides an alternative delivery form of parent training that allows practitioners to disseminate information and feedback at a distance. Telehealth can be as effective as in-person training (Wacker et al., 2013a; Wacker et al., 2013b; Vismara et al., 2009). Despite most telehealth studies indicating high satisfaction, research on patient satisfaction using telehealth has depended mostly on survey instruments, which limits definitive conclusions and may not translate into actual use or selection (Whitten & Love, 2005). The primary purpose of this study was to evaluate a procedure for identifying parent preference of in-person or telehealth training modalities. Secondary purposes were …

Land-Use & Water Quality In The Headwaters Of The Alafia River Watershed, Jaime Alison Swindasz

USF Tampa Graduate Theses and Dissertations

The objective of this study is to investigate land-use changes and water quality trends within the headwaters of the Alafia River watershed. Water quality data were obtained from the Environmental Protection Commission of Hillsborough County (EPCHC). Eleven water quality parameters selected for analysis included: temperature (˚C), dissolved oxygen (DO), percent saturation of DO, conductivity, pH, total phosphorous (TP), total nitrogen (TN), ammonium, chlorophyll-a (uncorrected), fecal coliforms, and enterococci. ArcMap® & SWFWMD data were used to map EPCHC sampling stations, calculate contributing watershed size, and determine land-use changes over the course of the sampling period; 17 stations were chosen for this …

To "Plant Our Trees On American Soil, And Repose Beneath Their Shade": Africa, Colonization, And The Evolution Of A Black Identity Narrative In The United States, 1808-1861, Edward Jason Vickers

USF Tampa Graduate Theses and Dissertations

This work explores the role that ideas about Africa played in the development of a specifically American identity among free blacks in the United States, from the early nineteenth century to the Civil War. Previous studies of the writings of free blacks in the Revolutionary period, and of the American Colonization Society (ACS), which was devoted to removing them back to an African homeland, have suggested that black discussions of Africa virtually disappeared after 1816, when the colonization movement began. However, as this work illustrates, the letters, books, newspapers, and organizational records produced by free blacks in the antebellum era …

Identifying Humanized Ecosystems: Anthropogenic Impacts, Intentionality, And Resource Acquisition At Crystal River (8ci1) And Roberts Island (8ci41), Charles Trevor Duke

USF Tampa Graduate Theses and Dissertations

The impact of human activity on ecosystems is an issue at the forefront of global concern. Marine ecosystems are a particular concern, given their importance for human sustenance. Through the removal of species that are highly susceptible to the effects of overfishing, global fisheries have been driven to near collapse in recent decades. The long-term effects of such practices has resulted in declines in mean trophic level of aggregate fish catches over time, as well as decreasing diversity of species available for regular harvest (Jackson et al. 2001; Pauly et al. 1998). These supposedly “modern” problems have been recently identified …

Basal Salivary Oxytocin And Skin To Skin Contact Among Lactating Mothers Of Premature Infants, Jessica Marie Gordon

USF Tampa Graduate Theses and Dissertations

This dissertation research explored mechanisms of human milk (HM) expulsion by describing the effects of skin to skin contact (SSC) on maternal basal oxytocin (OT) secretion among 20 premature mothers with hospitalized premature infants. This one-group, repeated measures design consisted of: 1) daily SSC with covariant data via self-report diary and 2) maternal salivary OT with and without SSC at 4 time points were collected over a 7 day time frame. Results indicate that mean levels of basal OT increase over time (M 234 pg/ml, SD 108 pg/ml time point 1; M 257 pg/ml, SD 125 pg/ml time point 3). …

Emergent Insect And Neotropical Migratory Bird Interactions And Responses To Habitat, Hydrology, And Progressive Urbanization In The Tampa Bay Region, Nathaniel Lee Goddard

USF Tampa Graduate Theses and Dissertations

The growing human population threatens the many of the earth’s biological systems. In the last 600 years extinction rates risen from 1 extinction per million species per year (E/MSY) in the 1400’s to 50 E/MSY today. During this time period 1.5% of all known birds have gone extinct, because they could not adapt quickly enough to human mediated changes. The goal of this dissertation was to determine how urbanization and anthropogenic services needed to support urban areas impact cypress dome wetland aquatic insect and migratory bird populations that depend on them. In Central Florida cypress dome hydroperiods are driven by …

The Cultivation Of Eating Disorders Through Instagram, Kendall O'Brien

USF Tampa Graduate Theses and Dissertations

A profusion of research has been dedicated to the effects of new media on body image. In an attempt to explain the inflation of eating disorders, several researchers have turned to the cultivation theory, postulating that increased interaction with these medias will lead to internalizations of the messages they disseminate. The over presence of extremely thin models and actresses can create a new reality for media users, who begin to equate thinness with beauty, power, femininity and happiness. While an abundance of research has delved into the impact of this thin ideal through television and magazines, the Internet as a …

Using Auditory Feedback To Teach Dance Skills To Adults With Intellectual Disabilities, Aracely Abreu

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to evaluate the use of auditory feedback for teaching individuals with intellectual disabilities the “Mississippi Cha Cha Slide.” Participants consisted of six males ages 35 to 61. During baseline, line dance skills were low for all participants. During the auditory feedback intervention, the trainer used a clicker to reinforce dance steps and forward chaining to chain movements into a sequence. Once auditory feedback was implemented, line dance skills increased substantially for all participants. Generalization assessments for four of the participants resulted in performance levels similar to baseline and demonstrate the need for future training …

High-Solids Anaerobic Digestion Of The Organic Fraction Of Municipal Solid Waste State Of The Art, Outlook In Florida, And Enhancing Methane Yields From Lignocellulosic Wastes, Gregory Richard Hinds

USF Tampa Graduate Theses and Dissertations

Anaerobic digestion (AD) is a biotechnology that employs natural microbial metabolism under oxygen-free conditions to stabilize organic waste. AD has been shown to be the most environmentally sustainable technology for treating the organic fraction of municipal solid waste (OFMSW), as it allows for the recovery of energy and nutrients from the waste. AD of OFMSW also saves landfill space and reduces leachate generation and fugitive methane emissions from landfills. High-solids AD (HS-AD) technologies (those designed to process feedstocks with >15% total solids content) have been shown to yield additional benefits when compared with liquid AD (L-AD) for treating OFMSW, including …

A Bat-Guano-Derived Δ15N And Δ13C Record Of Paleoenvironmental Change: Zidită Cave, Romania, Daniel Martin Cleary

USF Tampa Graduate Theses and Dissertations

Because nitrogen isotopes are fractionated along the soil-plant-insect-bat-guano pathway, it may be possible to reconstruct environmental and climatic changes reflected in the nitrogen isotopic composition of guano. A 1.5-m core of bat guano from Zidită Cave (western Romania) provides a record of climatic and anthropogenic influence on the regional nitrogen cycle and paleoenvironmental controls on nitrogen transforming processes. Increasing and decreasing trends of nitrogen isotopic composition (δ¹⁵N values) correspond well with changes in the influence of farming practices, deforestation, and forest expansion. These influences likely had a significant effect on the openness of the nitrogen cycle, resulting in …

Smartphone Based 3d Printed Colorimeter For Biomedical Applications, Karthik Raj Konnaiyan

USF Tampa Graduate Theses and Dissertations

Here we present a novel Smartphone-based colorimeter and demonstrate its application to the measurements of glucose and protein concentrations in biological samples. The key innovation of our approach was to combine powerful image processing encoded into a mobile phone application with a low cost 3D printed sample holder that allowed to control lighting conditions and significantly improved sensitivity. Different solutions with protein and glucose concentrations ranging from 0 to 2000 mg/dL were prepared and analyzed using our system. The Smartphone-based colorimeter always correctly classified the corresponding reagent strip pads, what confirms that it can be used as a low cost …

How Overqualification Impacts Job Attitudes And Well-Being: The Unique Roles Of Perceptions And Reality, Marijana L. Arvan

USF Tampa Graduate Theses and Dissertations

The recent global economic downturn has stimulated a growing interest among scholars in how employees interpret and respond to the circumstance of being overqualified. However, the overqualification literature has been hindered by uncertainty regarding the extent to which employees’ perceptions of being overqualified are based in reality. The present study sought to address this concern by proposing and testing a theoretical model of objective overqualification, perceived overqualification, job satisfaction, and well-being using a cross-sectional sample of full-time employees who had recently graduated from college. Additionally, the present study investigated cognitive ability, achievement striving, and trait negative affectivity as potential moderators …

Restricted And Repetitive Behaviors As Strengths, Not Weaknesses: Evaluating The Use Of Social Stories That Embed Restricted Interests On The Social Skills Of Children With Autism Spectrum Disorder, Maya Nasr

USF Tampa Graduate Theses and Dissertations

The purpose of this study was to investigate the extent to which the use of social stories that integrate a child’s particular restricted and repetitive behaviors results in differential social outcomes compared to the use of social stories that do not integrate restricted and repetitive behaviors. A non-concurrent multiple baseline experimental design across participants was used to examine the effects of two Social Story interventions on the frequency of appropriate social behaviors made by participants in a school setting. Field notes were also completed during each day of data collection in order to document the social context, events, activities, moods …

Structure Based Drug Design Targeting Bacterial Antibiotic Resistance And Alzheimer's Disease, Eric Michael Lewandowski

USF Tampa Graduate Theses and Dissertations

Structure based drug design is a rapidly advancing discipline that examines how protein targets structurally interact with small molecules, or known inhibitors, and then uses this information to lead inhibitor optimization efforts. In the case of novel inhibitors, protein structural information is first obtained via X-ray crystallography, NMR studies, or a combination of both approaches. Then, computational molecular docking is often used to screen, in silico, millions of small molecules and calculate the potential interactions they may have with the target protein’s binding pocket, in hopes of identifying novel low affinity inhibitors. By examining the interactions these small, low affinity, …

Role Of The Slingshot-Cofilin And Ranbp9 Pathways In Alzheimer's Disease Pathogenesis, Jung A Woo

USF Tampa Graduate Theses and Dissertations

Alzheimer’s disease (AD) is a neurodegenerative disorder characterized by two major pathological hallmarks, amyloid plaques and neurofibrillary tangles. The accumulation of amyloid-β protein (Aβ) is an early event associated with synaptic and mitochondrial damage in AD. Therefore, molecular pathways underlying the neurotoxicity and generation of Aβ represent promising therapeutic targets for AD. Recent studies have shown that actin severing protein, Cofilin plays an important role in synaptic remodeling, mitochondrial dysfunction, and AD pathogenesis. However, whether Cofilin is an essential component of AD pathogenesis and how Aβ induced neurotoxicity impinges its signals to Cofilin are unclear.

In my dissertation studies, we …

Characterization And Diurnal Measurement Of Oral Inflammation In Association With Glycemic Control, Periodontal Status, & Glucose Stimulation, Melanie N. Kuehl

USF Tampa Graduate Theses and Dissertations

Diabetes has afflicted 8.3%, approximately 25.8 million, of the United States population and is the seventh leading cause of death [1]. Type I diabetes (T1D) accounts for 5 to 10% of all diagnosed cases of diabetes in the United States [2]. If present trends continue, the rate of T1D incidence among children under the age of 14 will increase by 3% globally [3]. T1D is an autoimmune disorder in which the β-cells of the pancreatic islets are destroyed, leading to high blood sugar. Hyperglycemia and loss of immunological tolerance to self-antigens are common associations of T1D [4]. Periodontal disease impacts …

“Principal Component Analysis And The Cumulative Gait Index: Translational Tools To Assess Gait Impairments In Rats With Olivocerebellar Ataxia”, Chase Lambert

USF Tampa Graduate Theses and Dissertations

Numerous studies suggest that modulation of the cholinergic system through the use of nicotinic agonists can improve motor function in humans or animals with motor disorders. Specifically, although there are no approved therapeutics for patients with ataxia, the nicotinic receptor agonist varenicline has demonstrated efficacy to improve coordination and gait in several groups of patients with different subtypes of ataxia. Importantly, the mechanism underlying the varenicline’s mechanism of action to improve motor function remains to be elucidated. Thus, the purpose of these experiments was to first quantify gait impairments in rats with olivocerebellar ataxia utilizing an objective treadmill-based system to …

Evaluation Of Soil As A Risk Indicator For Human Leptospirosis In Coastal, Rural Ecuador, Chad Allen Weddell

USF Tampa Graduate Theses and Dissertations

Leptospirosis, a zoonotic disease caused by pathogenic spirochete bacteria (family Leptospiraceae, genus Leptospira), is endemic in developing tropical regions of the world. It occurs in epidemics and is endemic in Ecuador where environmental conditions are ideal for maintenance. The role of soil as a long term reservoir has been previously been documented. Geographic Information System (GIS) and Remote Sensing (RS) technology was used in our study to further explore the role of soil as an environmental reservoir and its potential use as a static risk indicator for disease. Red, Green, Blue (RGB) spectral band data from known leptospire …