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Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Pharmacokinetics And Bioavailability Of Three Promising Tilmicosin-Loaded Lipid Nanoparticles In Comparison With Tilmicosin Phosphate Following Oral Administration In Broiler Chickens, Ali Rassouli, Alwan Al-Qushawi, Fatemeh Atyabi, Seyed Mostafa Peighambari, Mehdi Esfandyari-Manesh, Gholam Reza Shams
Turkish Journal of Veterinary & Animal Sciences
Tilmicosin (TLM) is a semisynthetic antimicrobial agent used mainly in poultry and cattle, but it has relatively poor oral bioavailability. This study was conducted to compare the bioavailability (BA) and main pharmacokinetic (PK) parameters of TLM after oral administration of tilmicosin phosphate (TLM-PH) and three newly prepared lipid nanoparticles (LNPs) of TLM including solid lipid nanoparticles (SLNs), nanostructured lipid carriers (NLCs), and lipid-core nanocapsules (LNCs). Sixty broiler chickens were divided into eight groups. In four treatment groups (n = 10), each bird was given a single oral dose (20 mg/kg) of a TLM formulation after overnight fasting, and in four …
Hemopoiesis In The Pronephros Of Tench, Tinca Tinca, Linnaeus 1758(Teleostei, Cyprinidae): Cytochemical Identification And Cell Morphology, Andi Alijagic, Damir Suljevic
Hemopoiesis In The Pronephros Of Tench, Tinca Tinca, Linnaeus 1758(Teleostei, Cyprinidae): Cytochemical Identification And Cell Morphology, Andi Alijagic, Damir Suljevic
Turkish Journal of Veterinary & Animal Sciences
Hemopoiesis in the tench (Tinca tinca: Teleostei, Cyprinidae), a cyprinid fish that can be found within the entire territory of Eurasia, from France in the west to the Yenisei River in the east, has not yet been described. The aim of this research was analysis of hemopoiesis lineages in the pronephros of Tinca tinca (sampled from the Jablanicko Reservoir). Identification and cells measurements were done by using a light microscope (Olympus BX41) and an Olympus DP12 camera. Hemopoietic cells of the tench are divided into five different series that include erythropoietic, granulopoietic, lymphopoietic, thrombopoietic, and monopoietic cell series. Erythropoietic series …
Differences In Drone Sperm Morphometry And Activity At The Beginning And End Of The Season, Aldona Gontarz, Dorota Banaszewska, Magdalena Gryzinska, Katarzyna Andraszek
Differences In Drone Sperm Morphometry And Activity At The Beginning And End Of The Season, Aldona Gontarz, Dorota Banaszewska, Magdalena Gryzinska, Katarzyna Andraszek
Turkish Journal of Veterinary & Animal Sciences
The sperm cells of the honey bee, like the sperm of most invertebrates, belong to the modified type of spermatozoa. In our own research, differences have been observed in the activity of sperm cells isolated from drones at the beginning and end of the season. On the basis of these observations, research was undertaken to determine whether the changes in drone activity are associated with morphological changes. The sperm cells collected from drones at the beginning of the mating season are longer than the sperm cells from the end of the season. Moreover, sperm cells from the beginning of the …
Investigation Of The Promoter Polymorphisms Of The Growth Hormone (Gh1), Growth Hormone Receptor (Ghr), Insulin-Like Growth Factor (Igf-I), And Prolactin (Prl) Genes And The Correlation Between Gene Expression And Milk Yields In Holstein Cattle Raised In Central Anatolia, Korhan Arslan, Serpi̇l Taheri̇, Eli̇f Funda Şener, Bi̇lal Akyüz, Aytaç Akçay, Yusuf Özkul, Kaan Muhsi̇n İşcan
Investigation Of The Promoter Polymorphisms Of The Growth Hormone (Gh1), Growth Hormone Receptor (Ghr), Insulin-Like Growth Factor (Igf-I), And Prolactin (Prl) Genes And The Correlation Between Gene Expression And Milk Yields In Holstein Cattle Raised In Central Anatolia, Korhan Arslan, Serpi̇l Taheri̇, Eli̇f Funda Şener, Bi̇lal Akyüz, Aytaç Akçay, Yusuf Özkul, Kaan Muhsi̇n İşcan
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to investigate the promoter polymorphisms of the growth hormone (GH1), growth hormone receptor (GHR), insulin-like growth factor (IGF-I), and prolactin (PRL) genes, and the correlation of the expression levels of these genes with lactation and daily milk yields in Holstein cattle. A total of 154 lactating Holstein cows raised in the Central Anatolia Region of Turkey, with a mean age of 5.4 years and a mean body weight of 571.7 kg, constituted the study material. Each blood sample was examined by real-time PCR for mRNA expression levels and the promoter polymorphisms of the GH1, …
Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç
Molecular And Seroprevalence Of Canine Visceral Leishmaniasis In West Anatolia, Turkey, Serkan Bakirci, Hüseyi̇n Bi̇lgi̇n Bi̇lgi̇ç, Onur Köse, Ayça Aksulu, Seli̇n Hacilarlioğlu, Hasan Erdoğan, Tüli̇n Karagenç
Turkish Journal of Veterinary & Animal Sciences
Canine leishmaniasis (CanL) is a protozoal disease caused by Leishmania species. Dogs play an important role as a reservoir. The aim of this study is to determine the prevalence of CanL by molecular and serological techniques among stray dogs living in kennels in Aydın, Manisa, and İzmir, located in western Turkey. Blood samples were collected from a total of 191 dogs and analyzed using indirect immunofluorescence antibody test (IFAT) and polymerase chain reaction (PCR). The isolates that tested positive using PCR were further analyzed by real-time PCR for species identification and confirmation. A total of 21 (10.99%) dogs were found …
Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum
Ovine Coccidiosis: Prevalence And Associated Risk Factors Inand Around Addis-Zemen, Northwest Ethiopia, Abinet Lakew, Zewdu Seyoum
Turkish Journal of Veterinary & Animal Sciences
This cross-sectional study was conducted in and around Addis-Zemen from November 2014 to April 2015 to determine the prevalence of ovine coccidiosis and to identify associated risk factors. Faecal samples were collected directly from the rectum of 384 randomly selected animals. A centrifugal faecal flotation technique using saturated salt solution was used to detect Eimeria oocysts. Out of 384 samples, only 88 were found to contain Eimeria oocysts. Eimeria infection was recorded in all age and sex groups of the study animals. A strong significant association was observed (P < 0.05) between Eimeria infection and the faecal consistency, production system, hygienic status, age, and body condition of the sampled animals. However, an insignificant (P > 0.05) association was recorded between Eimeria infection and the origins and …
The Effects Of Carvacrol And/Or Thymol On The Performance, Blood And Rumen Parameters, And Carcass Traits Of Merino Sheep, Hakan Bi̇ri̇ci̇k, Hülya Hanoğlu Oral, Ali̇ Mehmet Taluğ, Şeri̇fe Şule Cengi̇z, Mehmet Koyuncu, Serdal Di̇kmen
The Effects Of Carvacrol And/Or Thymol On The Performance, Blood And Rumen Parameters, And Carcass Traits Of Merino Sheep, Hakan Bi̇ri̇ci̇k, Hülya Hanoğlu Oral, Ali̇ Mehmet Taluğ, Şeri̇fe Şule Cengi̇z, Mehmet Koyuncu, Serdal Di̇kmen
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the effects of increasing doses of carvacrol (C) and/or thymol (T) on the performance, rumen fermentation, and blood and carcass parameters of Merino sheep. Eighty-four 12-week-old male Merino lambs were randomly assigned to 7 treatment groups. The sheep were fed with the same concentrate mixtures including a control diet, carvacrol 100 mg/kg (C100), carvacrol 300 mg/kg (C300), thymol 100 mg/kg (T100), thymol 300 mg/kg (T300), carvacrol+thymol 100 mg/kg (C50+T50), and carvacrol+thymol 300 mg/kg (C150+T150). The C and/or T supplementation did not affect the feed conversion. The lambs fed with C and/or T …
The Determination Of In Vitro Gas Production And Metabolizable Energy Value Of Rice Straw Treated With Exogenous Fibrolytic Enzymes, Zehra Selçuk, Nurcan Çeti̇nkaya, Mustafa Salman, Buğra Genç
The Determination Of In Vitro Gas Production And Metabolizable Energy Value Of Rice Straw Treated With Exogenous Fibrolytic Enzymes, Zehra Selçuk, Nurcan Çeti̇nkaya, Mustafa Salman, Buğra Genç
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of cellulase and xylanase treatments on in vitro 24-h gas production (GP), organic matter digestibility (OMD), and metabolizable energy (ME) values of rice straw (RS). Rice straws were treated with cellulase and xylanase at the levels of 0% (C1), 0.5% (RS+CEL0.5), 1% (RS+CEL1), 1.5% (RS+CEL1.5), and 2% (RS+CEL2) and 0% (C2), 0.5% (RS+XYL0.5), 1% (RS+XYL1), 1.5% (RS+XYL1.5), and 2% (RS+XYL2), respectively. Rice straws treated with each level of cellulase and xylanase were ensiled in 6 glass jars. For each level of cellulase and xylanase treatment, 3 glass jars were incubated at room …
Effects Of Dietary Chicken Grill And Sunflower Seed Oils On Performance, Egg Yolk Cholesterol Level, Biochemical Parameters, And Oxidant/Antioxidant Status Of Laying Hens, Mustafa Numan Oğuz, Fatma Karakaş Oğuz, Tülay Büyükoğlu
Effects Of Dietary Chicken Grill And Sunflower Seed Oils On Performance, Egg Yolk Cholesterol Level, Biochemical Parameters, And Oxidant/Antioxidant Status Of Laying Hens, Mustafa Numan Oğuz, Fatma Karakaş Oğuz, Tülay Büyükoğlu
Turkish Journal of Veterinary & Animal Sciences
The objective of this study was to investigate the effects of dietary chicken grill oil (CGO) and sunflower seed oil (SO) on performance, some serum and egg yolk biochemical parameters, and blood and liver oxidant/antioxidant status of laying hens. Fifty-four 19-week-old Lohmann laying hens were randomly assigned to three groups. The groups were fed experimental diets containing 5% SO, 5% CGO1, or 7.5% CGO2, respectively. Diets and water were provided ad libitum over 17 weeks. The feed efficiency ratios and cholesterol content in egg yolks with cholesterol and the triglyceride levels in serum were not affected by the diets. However, …
Occurrence Of Arcobacter In Dogs And Cats In Selangor,Malaysia, And Associated Risk Factors, Dauda Mohammed Goni, Saleha Abdulaziz, Gurmeet Kaur Dhaliwa, Zunita Zakaria, Ibrahim Jalo Muhammad, Mohamed Abdelrahman Mohamed, Abdulrasheed Aliyu Bello, Asinamai Athliamai Bitrus
Occurrence Of Arcobacter In Dogs And Cats In Selangor,Malaysia, And Associated Risk Factors, Dauda Mohammed Goni, Saleha Abdulaziz, Gurmeet Kaur Dhaliwa, Zunita Zakaria, Ibrahim Jalo Muhammad, Mohamed Abdelrahman Mohamed, Abdulrasheed Aliyu Bello, Asinamai Athliamai Bitrus
Turkish Journal of Veterinary & Animal Sciences
Arcobacter is widely regarded as an emerging foodborne pathogen because of its increasing occurrence in food production causing gastroenteritis in humans. In addition, it is also reported as a potential zoonosis. The objectives of this study were to determine the occurrence of Arcobacter in dogs and cats and the associated risk factors. Rectal and buccal cavity swab samples were taken from dogs (n = 40) and cats (n = 40) owned by individuals who brought the animals to a university teaching hospital and stray dogs (n = 61) and cats (n = 46). Suspected colonies of Arcobacter were subjected to …
Circulation Of Schmallenberg Virus In Turkey, 2013, Şükrü Tonbak, Ahmet Kürşat Azkur, Züleyha Pesti̇l, Emel Biyikli, Hasan Abayli, Ersoy Baydar, Wim H.M. Van Der Poel, Hakan Bulut
Circulation Of Schmallenberg Virus In Turkey, 2013, Şükrü Tonbak, Ahmet Kürşat Azkur, Züleyha Pesti̇l, Emel Biyikli, Hasan Abayli, Ersoy Baydar, Wim H.M. Van Der Poel, Hakan Bulut
Turkish Journal of Veterinary & Animal Sciences
Schmallenberg virus (SBV) infection emerged in European domestic and wild ruminants in 2011. There is very limited information about the characterization of SBV isolates and the epidemiology of its infections in the rest of world, except for in European countries. We investigated the circulation of SBV in cattle herds in Central Anatolia, Turkey, in 2013. A total of 180 whole-blood samples were analyzed using real-time RT-PCR. The presence of SBV RNA was detected in 6 (3.3%) samples. For phylogenetic analysis and confirmation of real-time RT-PCR results, the S gene segment was amplified, sequenced, and compared to other segments. In addition, …
Comparison Of The Development Of Mouse Embryos Manipulatedwith Different Biopsy Techniques, Ali̇ Ci̇han Taşkin, Tolga Akkoç, Hakan Sağirkaya, Haydar Bağiş, Sezen Arat
Comparison Of The Development Of Mouse Embryos Manipulatedwith Different Biopsy Techniques, Ali̇ Ci̇han Taşkin, Tolga Akkoç, Hakan Sağirkaya, Haydar Bağiş, Sezen Arat
Turkish Journal of Veterinary & Animal Sciences
Preimplantation genetic diagnosis is the detection of inherited diseases and the sex of embryos before implantation in the practice of human medicine as well as in veterinary medicine. The introduction of experimental animal embryo biopsy techniques has been a milestone in the developmental process of preimplantation genetic diagnosis techniques. The aim of the present study was to evaluate in vivo and in vitro development of embryos after biopsy in an experimental mouse model and to perform comparisons across different biopsy techniques (blastomere biopsy and trophectoderm biopsy). At the end of the study, no significant difference was observed between the blastomere …
Estimation Of Stray Dog And Cat Populations In Metropolitan Ankara, Turkey, Doğukan Özen, Dankmar Böhning, İsmayi̇l Safa Gürcan
Estimation Of Stray Dog And Cat Populations In Metropolitan Ankara, Turkey, Doğukan Özen, Dankmar Böhning, İsmayi̇l Safa Gürcan
Turkish Journal of Veterinary & Animal Sciences
Stray dogs and cats contribute to serious health problems in human societies around the world. Before any necessary interventions to control the stray dogs and cats, an accurate estimate of their populations should be attained. Yet, there is a very limited number of methods for an estimation. Some of them depend on the identification of marked or counted animals. However, problems arise when it is not possible to identify a previously captured animal. In this paper, we used a different approach to estimate the lower bound for the total number of dogs and cats in consecutively visited settlements that might …
Evaluation Of Renal And Hepatic Functions In Cattle With Subclinical And Clinical Ketosis, Mustafa İssi̇, Yusuf Gül, Onur Başbuğ
Evaluation Of Renal And Hepatic Functions In Cattle With Subclinical And Clinical Ketosis, Mustafa İssi̇, Yusuf Gül, Onur Başbuğ
Turkish Journal of Veterinary & Animal Sciences
The purpose of this study was to research the changes in renal and hepatic functions of high productive dairy cows diagnosed with subclinical and clinical ketosis. The material of the study consisted of 30 dairy cows of high milk production. Diagnosis of ketosis was made according to anamnesis, physical examination, urine ketone bodies, and serum β-hydroxybutyric acid levels. The animals were divided according to their serum β-hydroxybutyric acid levels into control, subclinical, and clinical ketosis groups, each of which included 10 cows. Serum glucose, urea, creatinine, total protein, aspartate amino transaminase (AST), and total bilirubin levels were determined with an …
The Effect Of Ovulatory Follicle Size At The Time Of Insemination Onpregnancy Rate In Lactating Dairy Cows, Abdulkadi̇r Keski̇n, Gülnaz Meci̇toğlu, Ebru Bi̇len, Bariş Güner, Abdülkadi̇r Orman, Hayretti̇n Okut, Ahmet Gümen
The Effect Of Ovulatory Follicle Size At The Time Of Insemination Onpregnancy Rate In Lactating Dairy Cows, Abdulkadi̇r Keski̇n, Gülnaz Meci̇toğlu, Ebru Bi̇len, Bariş Güner, Abdülkadi̇r Orman, Hayretti̇n Okut, Ahmet Gümen
Turkish Journal of Veterinary & Animal Sciences
The aims of this study were to determine the factors that affect follicle size at the time of artificial insemination (AI) and to detect the relationship between ovulatory follicle size at the time of AI and pregnancy rate in dairy cows. A total of 1428 follicle size measurements were obtained from Holstein-Friesian (HF) and Swedish Red (SR) cows that were inseminated with the fixed-timed AI protocols used in this study. Follicle size was affected by breed (P = 0.0001), milk production (P = 0.01), parity (P = 0.05), and season (P = 0.04). Follicle size was greater (P = 0.0001) …
Comparative Evaluation Of Factors Affecting Hemagglutinatingactivity Of Avian Influenza (H9) Virus, Asfa Rasool, Sameera Akhtar, Khushi Muhammad, Masood Rabbani, Aftab Ahmed Anjum, Javed Muhammad, Arfan Ahmad, Ali Ahmad Sheikh, Fakhra Liaqat, Fariha Akhtar, Rabia Tahir
Comparative Evaluation Of Factors Affecting Hemagglutinatingactivity Of Avian Influenza (H9) Virus, Asfa Rasool, Sameera Akhtar, Khushi Muhammad, Masood Rabbani, Aftab Ahmed Anjum, Javed Muhammad, Arfan Ahmad, Ali Ahmad Sheikh, Fakhra Liaqat, Fariha Akhtar, Rabia Tahir
Turkish Journal of Veterinary & Animal Sciences
Influenza virus H9 was standardized for hemagglutination assay using different factors such as red blood cell (RBC) types, concentrations, diluent types, and storage times. Avian influenza virus H9 was grown in embryonated chicken eggs and confirmed by spot agglutination. A significant (P < 0.05) difference with the highest mean titer (9.00 ± 0.00) was observed using RBCs from different species. Nonsignificant differences (P > 0.05) were found between human blood type O, chicken, and dog RBCs, as well as among rabbit, pigeon, sheep, and parrot. The highest titer (9.00 ± 0.00) with a nonsignificant difference was found using virus stored at 4 °C and -20 °C while 37 °C showed the lowest significant mean hemagglutinin (HA) titer (11.08 ± 188.21). Nonsignificant differences …
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Immunohistochemical Evaluation Of Canine And Felinemerkel Cell Tumors?A Report Of Two Cases, Mateusz Mikiewicz, Iwona Otrocka-Domagala, Katarzyna Pazdzior-Czapula, Michal Gesek, Anna Mikolajczyk, Agnieszka Blacha
Turkish Journal of Veterinary & Animal Sciences
Merkel cell tumors, a type of cutaneous round-cell tumors, are rarely observed in dogs and cats. This tumor type originates from cutaneous neuroendocrine cells. In the present study, the morphology of canine and feline Merkel cell tumors and their immunoexpression of neuron-specific enolase, cytokeratin, chromogranin A, Ki67, and metallothionein were evaluated using histopathological and immunohistochemical methods. The canine Merkel cell tumor displayed a diffuse morphological pattern, whereas the feline tumor displayed a solid morphological pattern. Both tumors were positive for neuron-specific enolase and cytokeratin, but negative for chromogranin A. The Ki67 indexes in the canine and feline tumors were 7% …
Effects Of Mycobacterium Phlei On Intestinal Microecology Andserum Immunological Parameters Of Weaned Piglets, Jin-Feng Zhong, Xiao-Qing Zhang, Wei-Gao Wu, Wei Tu, Zhen-Xiang Liu, Re-Jun Fang
Effects Of Mycobacterium Phlei On Intestinal Microecology Andserum Immunological Parameters Of Weaned Piglets, Jin-Feng Zhong, Xiao-Qing Zhang, Wei-Gao Wu, Wei Tu, Zhen-Xiang Liu, Re-Jun Fang
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the effects of Mycobacterium phlei in diets (Experiment 1) and investigate the mode of action on the intestinal microecology of weaned piglets (Experiments 1 and 2 ). In Experiment 1, a total of 96 commercial cross-bred pigs [(Duroc × Yorkshire) × Landrace], weaned at 21 days, were assigned to two treatments in a completely randomized design with a single factorial arrangement of 40 days of supplementation (nonsupplemented vs. supplemented with 0.4% heat-killed M. Phlei). The results showed that M. Phlei has the capability to improve the intestinal microecology of weaned piglets. The …
Ultrasonographic Features Of Medial Iliac And Jejunal Lymph Nodesin Apparently Healthy Dogs, Shriram Ganesan, Jitender Mohindroo, Pallavi Verma, Narinder Singh Saini
Ultrasonographic Features Of Medial Iliac And Jejunal Lymph Nodesin Apparently Healthy Dogs, Shriram Ganesan, Jitender Mohindroo, Pallavi Verma, Narinder Singh Saini
Turkish Journal of Veterinary & Animal Sciences
Ultrasonographic examination of medial iliac and jejunal lymph nodes were performed in 36 dogs presented to the Department of Surgery and Radiology for spaying and castration. Ultrasonographic features such as length, height in longitudinal plane, shape, ease of scanning, echotexture, and echogenicity were recorded. The medial iliac and jejunal lymph nodes were scanned consistently in all 36 dogs. However, the presence of gas in the intestines affected the ease of scanning of the lymph nodes. The majority of these lymph nodes were easy to scan, and had homogeneous and hypoechoic echogenicity. The dogs were grouped based on body weight and …
Seroepidemiological Survey Of Bovine Tick-Borne Infections In Theblack Sea Region Of Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur, Kadri̇ Zafer Karaer, Ayşe Çakmak, Ayşe Serpi̇l Nalbantoğlu, Cevat Ni̇sbet
Seroepidemiological Survey Of Bovine Tick-Borne Infections In Theblack Sea Region Of Turkey, Mustafa Açici, Cenk Soner Bölükbaş, Gökmen Zafer Pekmezci̇, Ali̇ Tümay Gürler, Şi̇nasi̇ Umur, Kadri̇ Zafer Karaer, Ayşe Çakmak, Ayşe Serpi̇l Nalbantoğlu, Cevat Ni̇sbet
Turkish Journal of Veterinary & Animal Sciences
Babesiosis, theileriosis, and anaplasmosis are the tick-borne diseases of cattle in most of the tropical areas and Turkey. A total of 270 cattle were randomly selected from 27 villages and 77 farms in the Black Sea Region of Turkey for the determination of infections by Babesia bigemina and Anaplasma marginale by using the indirect fluorescent antibody (IFA) test and the competitive enzyme-linked immunosorbent assay (cELISA). Of those, 242 and 256 sera were also analyzed for antibodies against Theileria annulata and Babesia bovis infections using the IFA test, respectively, during the period of June 2006 to June 2007. Of the 256 …
Lipid And Protein Metabolism Along With Oxidative Status Of Follicular Fluidthroughout The Estrous Cycle In Anatolian Water Buffalo, Oktay Yilmaz, Jerzy Jan Jaroszewski, Azi̇z Bülbül, Mehmet Uçar
Lipid And Protein Metabolism Along With Oxidative Status Of Follicular Fluidthroughout The Estrous Cycle In Anatolian Water Buffalo, Oktay Yilmaz, Jerzy Jan Jaroszewski, Azi̇z Bülbül, Mehmet Uçar
Turkish Journal of Veterinary & Animal Sciences
This study was performed to determine the concentrations of creatinine (CRE), high-density lipoprotein (HDL), low-density lipoprotein (LDL), blood urea nitrogen (BUN), nitric oxide (NO), and malondialdehyde (MDA) in follicular fluid (FF) in relation to follicular size and/or stage of the estrous cycle [early I luteal (n = 14), early II luteal (n = 16), late luteal (n = 16), and follicular (n = 16) phases] in Anatolian water buffaloes. The concentrations of CRE (P < 0.001), HDL (P < 0.001), LDL (P < 0.05) BUN (P < 0.001), and NO (P < 0.01) in FF of large follicles (LFs, ?10 to
Atopic Dog Skin Shows Decrease Of Claudin-1 But Increase Of Atopic Signature Cytokines, Sirin Theerawatanasirikul, Pakawadee Pongket, Lawan Larsuprom, Amornrate Sastravaha
Atopic Dog Skin Shows Decrease Of Claudin-1 But Increase Of Atopic Signature Cytokines, Sirin Theerawatanasirikul, Pakawadee Pongket, Lawan Larsuprom, Amornrate Sastravaha
Turkish Journal of Veterinary & Animal Sciences
Canine atopic dermatitis is a common allergic skin disease in dogs caused by defects in immunological and epidermal barriers. Claudin-1, a protein present in tight junctions, is associated with many skin conditions. Our aim was to investigate expression levels of claudin-1 and atopic signature cytokines in atopic and normal dog skins. The affected skin showed significantly decreased intensity and epidermal distribution of claudin-1 compared to the normal control skin (P < 0.05). The CD3+/CD4+ and CD3+/CD8+ T cell subsets, eosinophils, and neutrophils infiltrated the affected epidermis and dermis. The CD3+/CD4+ T cell subsets in lesional skin were significantly higher than CD3+/CD8+ T cell subsets. CLDN-1 mRNA expression was markedly downregulated in the lesional skin, while IL-17A was upregulated in both lesional and nonlesional skin. IL-4 and IL-31 mRNA was upregulated in the lesional skin, and TNF-α mRNA was significantly upregulated in the nonlesional skin (P < 0.01 and P < 0.05, respectively). Correlation-based hierarchical clustering showed that CLDN-1 expression was closely clustered with IL-31 but loosely clustered with L-4, IL-10, IL-13, and IL-17A. We suggest that the alteration of claudin-1 in atopic dogs may disrupt the skin barrier and allow an influx of allergens, inciting inflammatory cytokine responses.
Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim
Comparative Study Between Formalin-Killed Vaccine And Developed Gammairradiation Vaccine Against Mannheimia Haemolytica In Rabbits, Sahar Ahmed, Basem Ahmed, Ghada Mahmoud, Waleed Nemr, Emam Abdel Rahim
Turkish Journal of Veterinary & Animal Sciences
Mannheimia haemolytica is responsible for considerable economic losses to sheep, goats, and cattle and other livestock industries in Egypt. This study aimed to evaluate the effectiveness of a newly developed gamma irradiation vaccine against Mannheimia haemolytica in comparison to a formalin-killed vaccine. Three groups of rabbits were used in this study. Group 1 animals were inoculated with 4 × 109 bacterial cells per dose of the formalin-killed vaccine. Group 2 was inoculated with 2 × 109 bacterial cells per dose of gamma-irradiated vaccine. Group 3 (control group) was injected with 2 mL of sterile PBS. The vaccines were injected subcutaneously …
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
High Level Fluoroquinolone Resistance In Escherichia Coli Isolatedfrom Animals In Turkey Is Due To Multiple Mechanisms, Pinar Şahi̇ntürk, Erdem Arslan, Esra Büyükcangaz, Songül Sonal, Ayşi̇n Şen, Fi̇gen Ersoy, Mark A. Webber, Laura Jv. Piddock, Murat Cengi̇z
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine the molecular mechanisms of fluoroquinolone resistance in E. Coli isolated from cattle, goats, sheep, cats, and dogs in Turkey. Twenty nonreplicate E. Coli isolates (chosen on the basis of RAPD pattern) from food-producing animals were selected for the study. To identify phenotypic differences between isolates, the sum of the MIC values of 14 antimicrobials was calculated; values ranged from 565 to 2520 μg/mL, indicating the diversity in antimicrobial resistance present in the panel of isolates. PCR and qRT-PCR were used to characterize the presence and expression levels of known molecular mechanisms of …
Mycoflora And Chemotype Characterization Of Fusarium Graminearum Isolatesobtained From Corn And Barley Used As Feedstuffs In The Northeast Of Iran, Abdollah Salehi, Roya Rezaeian Doloei
Mycoflora And Chemotype Characterization Of Fusarium Graminearum Isolatesobtained From Corn And Barley Used As Feedstuffs In The Northeast Of Iran, Abdollah Salehi, Roya Rezaeian Doloei
Turkish Journal of Veterinary & Animal Sciences
This study aimed to investigate the mycoflora and chemotyping of Fusarium graminearum isolates in corn and barley used in industrial dairy farms in Khorasan-Razavi Province, northeastern Iran. For this purpose, a total of 128 corn and barley samples were collected from 16 different large industrial dairy farms. Mean moisture of the samples collected in different seasons of the year was significantly different (P < 0.05). The most prevalent fungi isolated from corn samples were Penicillium spp. (90.62%) and Aspergillus spp. (82.81%), and that of barley samples was Penicillium spp. (89.06%). Frequency of Fusarium spp. İn corn and barley samples was 85.93% and 26.56%, respectively. All isolates (100%) were confirmed as Fusarium using an ITS primer. Eight (11.11%) out of 72 Fusarium spp. İsolates were confirmed as F. Graminearum using species-specific primers. Five out of 8 F. Graminearum isolates were positive in terms of the presence of the trichothecene-producing gene using a Tri5-specific primer. Three and 2 out of 5 toxin-producing F. Graminearum isolates were positive using Tri13DON- and Tri13NIV-specific primers, respectively. In conclusion, the presence of toxigenic Fusarium in corn and barley samples used in feedstuffs in Iran is a potential risk to animal and human health.
Appraisal Of The Impact Of Aluminosilicate Use On The Health And Performance Of Poultry, Ali Gilani, Hassan Kermanshahi, Abolghasem Golian, Saeed Seifi
Appraisal Of The Impact Of Aluminosilicate Use On The Health And Performance Of Poultry, Ali Gilani, Hassan Kermanshahi, Abolghasem Golian, Saeed Seifi
Turkish Journal of Veterinary & Animal Sciences
Aluminosilicates are feed additives used for multiple purposes in the poultry industry. Bentonites, zeolites, and kaolins are types of aluminosilicate compounds that appear in the marketplace due to their capabilities as mycotoxin adsorbents and other qualifications. While there are some differences among their efficacies, anecdotal evidence confirms that these additives ameliorate aflatoxicosis and manure ammonia emission, and improve the durability of pelleted diets. Furthermore, these supplements are safe at 0.2%-1% inclusion levels in the commercial diet of broilers and layers and did not have any adverse effects on productive indices, and even promoted birds' performance. Nevertheless, their upper levels, especially …
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Comparison Of The Efficiency Of Concentrated Soluble Recombinant Phospholipase D And Natural Phospholipase D Enzymes, Asli Sakmanoğlu, Osman Ergani̇ş
Turkish Journal of Veterinary & Animal Sciences
Phospholipase D (PLD) is a major virulence determinant of Corynebacterium pseudotuberculosis. Various studies have focused on the expression of recombinant PLD (rPLD) enzyme in different Escherichia coli strains, but generally a high yield of insoluble protein was reported. The aims of this study were to express soluble rPLD by different methods in E. coli. The rPLD and natural PLD (dPLD) enzymes were concentrated using an ultramembrane cassette system after the efficiencies of these concentrated enzymes were compared. The rPLD enzyme was expressed in One Shot®BL21(DE3) E. coli when induced by IPTG in TY medium. Soluble dPLD and rPLD enzyme hemolytic …
Early Pregnancy Diagnosis Using A Commercial Elisa Test Based On Pregnancy-Associated Glycoproteins In Holstein-Friesian Heifers And Lactating Cows, Mehmet Sali̇h Kaya, Mehmet Köse, Faruk Bozkaya, Hasan Mutlu, Eyyüp Hakan Uçar, Mehmet Osman Atli
Early Pregnancy Diagnosis Using A Commercial Elisa Test Based On Pregnancy-Associated Glycoproteins In Holstein-Friesian Heifers And Lactating Cows, Mehmet Sali̇h Kaya, Mehmet Köse, Faruk Bozkaya, Hasan Mutlu, Eyyüp Hakan Uçar, Mehmet Osman Atli
Turkish Journal of Veterinary & Animal Sciences
In this study, we aimed to investigate the efficacy of a commercial ELISA test kit for detecting pregnancy-associated glycoproteins (PAGs) in peripheral blood for early pregnancy diagnosis and compare plasma PAG levels during early pregnancy in both Holstein-Friesian heifers and lactating cows. A total of 231 plasma samples were collected from heifers and lactating cows on days 25, 28, and 32 after insemination. Pregnancies were confirmed 30 days after the collection of plasma samples. Plasma PAG levels were measured using a commercial ELISA test kit for diagnosing bovine pregnancy. The effects of examination date and animal status (heifers vs. lactating …
Supplementation Effects Of Seleno-Compounds, Carnosic Acid, And Fish Oil Onconcentrations Of Fatty Acids, Tocopherols, Cholesterol, And Amino Acids In The Livers Of Lambs, Agnieszka Justyna Rozbicka-Wieczorek, Edyta Wiesyk, Katarzyna Agnieszka Krajewska-Bienias, Krzysztof Wereszka, Marian Czauderna
Supplementation Effects Of Seleno-Compounds, Carnosic Acid, And Fish Oil Onconcentrations Of Fatty Acids, Tocopherols, Cholesterol, And Amino Acids In The Livers Of Lambs, Agnieszka Justyna Rozbicka-Wieczorek, Edyta Wiesyk, Katarzyna Agnieszka Krajewska-Bienias, Krzysztof Wereszka, Marian Czauderna
Turkish Journal of Veterinary & Animal Sciences
Lambs were fed a diet containing 3% rapeseed oil (RO) (the RO diet), a diet with 2% RO and 1% fish oil (FO) (the FO diet), or diets with combined additions of 2% RO, 1% FO, 0.1% carnosic acid (CA) (the CA diet), and 0.35 ppm Se as selenized yeast (SeY) (the CASeY diet) or selenate (SeVI) (the CASeVI diet). The CASeVI diet most effectively increased the body mass gain of lambs, while it most efficiently decreased the liver mass. The CASeVI diet most efficiently reduced the concentration of cholesterol and the lipid peroxidation in the liver. The CA diet …
Selecting Eimeria Tenella-Resistance Markers By Comparing Resistant And Susceptible Groups Of Jinghai Yellow Chickens, Jingjing Zhang, Yuxin Lin, Guojun Dai, Tingting An, Mingming Sun, Genxi Zhang, Kaizhou Xie, Jingyu Wang
Selecting Eimeria Tenella-Resistance Markers By Comparing Resistant And Susceptible Groups Of Jinghai Yellow Chickens, Jingjing Zhang, Yuxin Lin, Guojun Dai, Tingting An, Mingming Sun, Genxi Zhang, Kaizhou Xie, Jingyu Wang
Turkish Journal of Veterinary & Animal Sciences
Improving the genetic resistance of chickens to parasitic diseases is an alternative way to protect the poultry industry. To establish valid measurable in vivo parameters indicating coccidiosis resistance, 232 Jinghai Yellow chickens were randomly selected and orally infected with Eimeria tenella (1.5 x 104 sporulated oocysts per chicken). The resistant and susceptible chickens were classified based on their cecal lesion scores 8 days after infection. Ten plasma components, including antioxidant enzymes, interleukins (ILs), nitric oxide (NO), interferon-γ (IFN-γ), and β-carotene, were compared among the resistant, susceptible, and control chickens (uninfected with E. tenella). The NO, superoxide dismutase (SOD), IL17, and …