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Effects Of Supplemental Dietary Chromium On Yield And Nutrient Digestibility Of Laying Hens Under Low Temperature, Kazim Şahi̇n, O. Ni̇hat Ertaş, Talat Güler, Mehmet Çi̇ftçi̇
Effects Of Supplemental Dietary Chromium On Yield And Nutrient Digestibility Of Laying Hens Under Low Temperature, Kazim Şahi̇n, O. Ni̇hat Ertaş, Talat Güler, Mehmet Çi̇ftçi̇
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to determine the effects of chromium picolinate (CrPi) added into diet containing 710.3 ppb chromium on yield and nutrient digestibility of laying hens at low temperature. Forty-six-week-old laying hens were randomly assigned to four groups of 30 hens per group. Treatment groups were fed different supplemental dietary chromium levels. Thus, hens were fed diets with no supplemental chromium (Control Group), 100 ppb of supplemental chromium (100 Group), 200 ppb of supplemental chromium (200 Group) and 400 ppb of supplemental chromium (400 Group). Diet chromium concentration was detected by graphite furnace atomic absorption spectroscopy. Body weight, feed …
Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen
Diagnosis Of Lymphoid Leukosis (Ll) By Elisa (Enzyme-Linked Immunosorbent Assay) And Marek's Disease (Md) By If (Immunofluorescence) Test In Chickens, Selda Özbi̇lgi̇n, Ayşi̇n Şen, Mi̇hri̇ban Ülgen, K. Tayfun Çarli, Gürsel Sönmez, Özlem Özmen
Turkish Journal of Veterinary & Animal Sciences
In this study, our aim was to diagnose lymphoid leukosis and Marek's disease, which are neoplastic diseases of chickens, by ELISA and Indirect Immunofluorescence Test (IIF), respectively. Samples (eggs and tissues) were selectively taken from the flocks suspected of having lymphoid leukosis and Marek's disease. The tissues were macroscopically and microscopically examined. Tissues belonging to some flocks showed typical lesions for lymphoid leukosis and Marek's disease. Seven broiler breede flocks and three commercial layer flocks were tested for avian leukosis virus (ALV) group specific antigen (p27) in the albumen. ALV p27 antigen was detected in five out of ten flocks. …
Multiple-Trait Analysis Using Bayesian Methods In Animal Breeding: A Gibbs Sampling Approach, Mehmet Zi̇ya Firat
Multiple-Trait Analysis Using Bayesian Methods In Animal Breeding: A Gibbs Sampling Approach, Mehmet Zi̇ya Firat
Turkish Journal of Veterinary & Animal Sciences
Most animal breeding experiments are based on more than one economically important trait measured in each individual. In the statistical analysis of the resulting data, traits recorded in the same individuals are often considered one at a time. Usually we are interested, however, not only in the mode of inheritance of a particular trait but also in its relationships with other traits. Multivariate analyses are required to make inferences about genetic and phenotypic correlations between traits, but point estimates of such parameters can be poor even when breeding data on hundreds of animals are used. Bayesian methods exclude variance matrices …
The Effects Of Thyroid Disorders On Some Haematologic Parameters In Quail, Mukaddes Özcan, Murat Arslan, Erdal Matur, Ülker Çöteli̇oğlu, Eli̇f Ergül
The Effects Of Thyroid Disorders On Some Haematologic Parameters In Quail, Mukaddes Özcan, Murat Arslan, Erdal Matur, Ülker Çöteli̇oğlu, Eli̇f Ergül
Turkish Journal of Veterinary & Animal Sciences
In this study, the effects of experimentally induced hyperthyroidism and hypothyroidism on the erythrocyte, leucocyte and haematocrit values, the amount of haemoglobin and plasma glucose, cholesterol, triglyceride, creatinine and lactate dehydrogenase (LDH) levels were investigated. A total of 45 4-week-old female Japanese quail (Coturnix coturnix Japonica) were used. The quails were divided into 3 groups as hyperthyroid, hypothyroid and the control group, with 15 quails in each. The birds in the control group were fed with unsupplemented commercial quail diet for 5 weeks. At a rate of 0.4mg/100g, Levatroxin-sodium was added into the diet of the hyperthyroid group and 0.2 …
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Cloning Of Bacillus Subtilis Rskk243 Bifunctional Xylanase Gene In E. Coli And B. Subtilis And Enzyme Characterization, Bahri̇ Devri̇m Özcan, Numan Özcan
Turkish Journal of Veterinary & Animal Sciences
A bifunctional gene xylanase of B. subtilis RSKK243 encoding detectable beta(1,4) glucanase (CMCase) activity in addition to a high level of main xylanase activity was cloned and expressed in Escherichia coli with pUC18 plasmid and in various Bacillus subtilis strains with pUB110 vector. For enzymatic analysis and plasmid isolation, the gene was also expressed in xylanase and beta glucanase negative B.subtilis YB886 strain with pUB110 vector. The pUB110 with bifunctional xylanase gene was transferred into B. subtilis RSKK243 for maximizing the enzyme yield, as well as into other RSKK strains to create novel strains with multiple enzyme capacities. Recombinant strains …
Canine Mast Cell Tumors: A Pathological Survey Of Nineteen Cases, Sevi̇l Atalay Vural, Yilmaz Aydin
Canine Mast Cell Tumors: A Pathological Survey Of Nineteen Cases, Sevi̇l Atalay Vural, Yilmaz Aydin
Turkish Journal of Veterinary & Animal Sciences
In the survey study, a total of 19 canine mast cell tumors were examined with regard to sex, age, breed of dogs, tumor location, and pathological findings in the period of 1960-1999. The majority of tumors occurred in males (78%) and between the ages of 6 and 11 years (67%). The pointer was predisposed to development of tumors (35%). The hindquarters and hind legs were most commonly involved, followed by the extragenital inguinal area and trunk. The tumors clearly had a site preference for the posterior region of the body (79%). The only extracutaneous tumor was found in the nasal …
The Effects Of Enzyme And Probiotic Supplementation To Diets On Broiler Performance, Mustafa Mi̇di̇lli̇, Şaki̇r Doğan Tuncer
The Effects Of Enzyme And Probiotic Supplementation To Diets On Broiler Performance, Mustafa Mi̇di̇lli̇, Şaki̇r Doğan Tuncer
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effect of the supplementation of enzyme and probiotic to broiler feeds based on barley and wheat on broiler performance, dressing percentage, the small intestinal length and weight, liver and abdominal fat weights, intestinal viscosity and the percentage of sticky droppings. In this experiment, 600 one - day - old Hubbard broiler chicks were used. This study was performed on four treatment groups, each containing 150 chicks. One of the groups was the control group and the others were the experimental groups. Each treatment group was divided into 6 replicates of 25 chicks. …
Use Of Sunflower Meal With Enzyme Mixture Substituted For Soybean Meal In Broiler Diets, Fi̇gen Kirkpinar, Hati̇ce Basmacioğlu
Use Of Sunflower Meal With Enzyme Mixture Substituted For Soybean Meal In Broiler Diets, Fi̇gen Kirkpinar, Hati̇ce Basmacioğlu
Turkish Journal of Veterinary & Animal Sciences
The study was carried out to investigate the effects of sunflower meal with an enzyme mixture containing \alpha-amylase, ß-glucanase, cellulase, lipase and protease substituted for soybean meal in broiler diets on performance, intestinal weight, viscosity and pH, as well as the frequency of sticky droppings and abdominal fat. In the trial, a total of 960 one-day-old Avian broiler chicks were used. The trial lasted 6 weeks. A total of 12 experimental diets were supplied ad libitum. Dietary treatments consisted of 15%, 25%, 35% soybean meal, or sunflower meal or sunflower meal with the enzyme mixture (0.05% and 0.10%). Enzyme supplementation …
Effects Of Copper Sulphate Administration To Pregnant Sheep On Some Mineral Levels In Blood Sera Of Sheep And Lambs, And Birth Weight Of Lambs, İbrahi̇m Çi̇mtay, Teki̇n Şahi̇n, Ali̇ Ölçücü, Gürbüz Aksoy
Effects Of Copper Sulphate Administration To Pregnant Sheep On Some Mineral Levels In Blood Sera Of Sheep And Lambs, And Birth Weight Of Lambs, İbrahi̇m Çi̇mtay, Teki̇n Şahi̇n, Ali̇ Ölçücü, Gürbüz Aksoy
Turkish Journal of Veterinary & Animal Sciences
In this study, copper sulphate was administered during the last period of pregnancy to sheep in the Şanlıurfa region in which enzootic ataxia was encountered in the past. The objectives of this study were to search the effect of copper sulphate administration on some mineral levels (copper, zinc, iron, calcium and magnesium) in blood serum of pregnant sheep and their lambs, and birth weight of lambs. This study was performed on 35 pregnant sheep (control group: 15, experimental group: 20). In the experimental group, copper sulphate (2% solution) was administered orally at sixth and fourth weeks before birth at 2 …
Survey Of European Foulbrood Disease In Bee Farms Located In Elazığ And The Surrounding Provinces, Hali̇l Şi̇mşek, Canan Özcan
Survey Of European Foulbrood Disease In Bee Farms Located In Elazığ And The Surrounding Provinces, Hali̇l Şi̇mşek, Canan Özcan
Turkish Journal of Veterinary & Animal Sciences
A total of 250 specimens from 110 bee farms located in Elazığ and the surrounding provinces were taken in 1999 and 2000. The specimens were examined for European Foulbrood Disease. Under macroscopic examination clinical symptoms were noted in 12 specimens, and Paenibacillus alvei was isolated and identified from 8 of these specimens. As a result of the antibiotic sensitivity test, the isolate was found sensitive to enrofloxacin, streptomycin and erythromycin 75%, 75%, and 62,5% of the time respectively. In this study, the presence of European Foulbrood Disease was determined in 3.2% of cases in Elazığ and the surrounding provinces.
Effects Of Copper Sulphate Administration On Body Weight Gain And Some Blood Parameters In Lambs, Teki̇n Şahi̇n, İbrahi̇m Çi̇mtay, Gürbüz Aksoy, Ali̇ Ölçücü
Effects Of Copper Sulphate Administration On Body Weight Gain And Some Blood Parameters In Lambs, Teki̇n Şahi̇n, İbrahi̇m Çi̇mtay, Gürbüz Aksoy, Ali̇ Ölçücü
Turkish Journal of Veterinary & Animal Sciences
The objectives of this study were to determine the effects of copper sulphate administration on body weight gain and some biochemical, haematological parameters in lambs which had serum copper levels at subclinical values. This study was performed on 30 Ivesi male lambs (control group: 12, experimental group: 18), aged with 15-20 days. In the experimental group, 100 mg copper sulphate (1% solution) was administered orally four times (with periods of two weeks). Blood samples were collected on 0, 14, 28, 42, 56th days and body weight of lambs was determined. Copper administration caused significant increases in weight gain, haemoglobin and …
Vitamin A, Vitamin E And Selenium Levels In Blood And Milk Sera Of Cattle With Foot And Mouth Disease, Serdar Kizil, Ari̇f Altintaş
Vitamin A, Vitamin E And Selenium Levels In Blood And Milk Sera Of Cattle With Foot And Mouth Disease, Serdar Kizil, Ari̇f Altintaş
Turkish Journal of Veterinary & Animal Sciences
The aim of this study was to determine \beta-carotene, retinol (Vit A), selenium (Se) and \alpha-tocopherol (Vit E) levels in the sera of blood and milk from cattle with foot and mouth disease (FMD). A total of 120 blood sera and 22 milk sera were used for the analysis. \beta-carotene and Vit A, Se and Vit E levels were determined by spectrophotometer, fluorophotometer and HPLC respectively. Serum samples were obtained from 0-1 and 1-3-year-old cattle bred in public and state farms. Animals were grouped according to their sex, vaccination status and health or infection with FMD. Results of the biochemical …
The Effect Of Genotype On The Antioxidative Metabolism In Angora Goats, Ulvi̇ Reha Fi̇danci, Fügen Turgay, Seda Zengi̇n, Funda Kargin, Sefa Çeli̇k, Umut Taşdemi̇r
The Effect Of Genotype On The Antioxidative Metabolism In Angora Goats, Ulvi̇ Reha Fi̇danci, Fügen Turgay, Seda Zengi̇n, Funda Kargin, Sefa Çeli̇k, Umut Taşdemi̇r
Turkish Journal of Veterinary & Animal Sciences
In this study, lipid peroxidation, intracellular, membrane and extracellular antioxidant activities in Turkish Angora goats (Genotype I) and American x Turkish crossbred Angora goats (Genotype II) were investigated, in order to show the effects of genotype on the antioxidative metabolism. Plasma malonlydialdehyde (MDA) levels of Angora goats in different genotype were analyzed to show the effects of free radicals. Glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities in hemolysates of erythrocytes were analyzed as intracellular antioxidants, \beta-carotene in serum as membrane antioxidants and ascorbic acid in plasma, albumin, total bilirubin, glucose, uric acid and ceruloplasmine in serum as …
Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci
Detection Of Antibodies Against Salmonella Enteritidis In Chicken Serum And Egg Yolk With Elisa, Gülay Altay, Hakan Yardimci
Turkish Journal of Veterinary & Animal Sciences
A total of 473 sera from hens of different age groups and different breeds and a total of 430 eggs were collected from 11 breeders and egg producers in the districts of Adapazarı, Afyon, Ankara and Konya. Lipopolysaccharide (LPS), heat-extract (HE) and flagella antigens were tested in the standardizing ELISA technique. Flagella antigen was found useful in the screening of field samples. Of the 48 (10.2%) rapid plate agglutination positive sera, 7 were negative in ELISA. One hundred and seventy-three (36.6%) of the 473 sera and 76 (17.7%) of the 430 egg yolks were found to be positive with ELISA. …
Photoinactivation Of Buffalo Liver And Kidney Tissue Arginases And Its Kinetic Properties, Necmi̇ Özdemi̇r, Mehtap Özçeli̇k
Photoinactivation Of Buffalo Liver And Kidney Tissue Arginases And Its Kinetic Properties, Necmi̇ Özdemi̇r, Mehtap Özçeli̇k
Turkish Journal of Veterinary & Animal Sciences
Exposure of buffalo liver and kidney tissue arginases to a 150 w light source from a distance of 8-10 cm, in the presence of 0.05mM methylene blue, caused significant inhibition of arginase activity. It was determined that this inhibition occurred differently in liver and kidney tissues. Arginase activity was spectrophotometrically measured using the Thiosemicarbazide diacetyl-monoxime urea (TDMU) method. Following preincubation with MnCl_2, exposure to a 150 w light source and the addition of methylene blue, the activity of arginase was found to be lower than that after preincubation with a 150 w light source and methylene blue and the addition …
Effects Of Administration Of Fertirelin Acetate And Gentamycin Sulphate After Artificial Insemination On Pregnancy Rate Of Cows, Ati̇lla Yildiz
Effects Of Administration Of Fertirelin Acetate And Gentamycin Sulphate After Artificial Insemination On Pregnancy Rate Of Cows, Ati̇lla Yildiz
Turkish Journal of Veterinary & Animal Sciences
Two studies were conducted to evaluate if Fertirelin acetate (an analogue of GnRH) and Gentamycin sulphate would improve pregnancy rate in healthy cows or in nonpregnant cows although they were inseminated three times or more. The first investigation was carried out in sixty-eight healthy cows. Cows were alloted randomly to one of two treatment groups or one of two control groups when found to be in oestrus. The first treatment group was treated with 100 micrograms of Fertirelin acetate, while the first control grup was given saline (2 ml) intramuscularly immediately after artificial insemination. The second treatment group was treated …
A Study On Some Production Traits And Egg Quality Characteristics Of Japanese Quail, Ahmet Nazligül, Kenan Türkyilmaz, Hüsnü Erbay Bardakçioğlu
A Study On Some Production Traits And Egg Quality Characteristics Of Japanese Quail, Ahmet Nazligül, Kenan Türkyilmaz, Hüsnü Erbay Bardakçioğlu
Turkish Journal of Veterinary & Animal Sciences
This investigation was carried out to demonstrate some production traits and egg quality characteristics of Japanese quails (Coturnix coturnix japonica). One hundred female quails were used in the study. The experiment was carried out for six months. Egg production, egg weight, feed consumption and some egg quality characteristics were taken into consideration. Egg quality characteristics were measured at 8-24 weeks of age. Average egg production (% HD) at 8, 12, 16, 20 and 24 weeks of age was 20.5, 33.8, 63.7, 73.8 and 72.5 respectively. Average egg weight (g) and daily feed consumption (bird/feed/day) at 8, 16 and 24 weeks …
The Possibilities Of Using Sugar Beet Pulp Silage Produced By Different Methods In Lamb And Dairy Cow Rations 1 - Obtaining High Quality Sugar Beet Pulp Silage, Suphi̇ Deni̇z, Murat Demi̇rel, Şaki̇r Doğan Tuncer, Oktay Kaplan, Taylan Aksu
The Possibilities Of Using Sugar Beet Pulp Silage Produced By Different Methods In Lamb And Dairy Cow Rations 1 - Obtaining High Quality Sugar Beet Pulp Silage, Suphi̇ Deni̇z, Murat Demi̇rel, Şaki̇r Doğan Tuncer, Oktay Kaplan, Taylan Aksu
Turkish Journal of Veterinary & Animal Sciences
In this study, how to obtain high quality sugar beet pulp silage was investigated. Sugar beet pulp was mixed with wheat straw or grass hay in order to increase the level of dry matter to 20, 25 and 30%, and 5% molasses and different levels of urea were added (0, 1, 1.5 and 2% for wheat straw containing groups; 0, 0.5 and 1% for grass hay containing groups). The mixed materials were stored in containers of one kg for two months. At the end of the incubation period, the containers were opened and the silages were evaluated by measuring the …
The Effects Of Narrowed Area And Additional Feeding On Some Physiological Characteristics Ofhoney Bee (Apis Mellifera L.) Colonies, Ahmet Güler
Turkish Journal of Veterinary & Animal Sciences
The study was conducted at Alata Horticultural Research institute between 1994 and 1996. The aim of the study was to obtain a strong colony by way of supersedure and empty frames, feeding with saccharose syrup and encouraging egg laying of queen bees at the end of summer. According to the results, there were significant differences among treatments in terms of the development of colony population, brood rearing activity and honey yield. Treatments had no significant effect on wax building activity and swarming tendency.
Fattening Performance And Carcass Characteristics Of Awassi Crosses (F1 And B1) With German Blackheaded Mutton And Hampshire Down Male Lambs, Ali̇ Akmaz, Emi̇n Teki̇n, Ramazan Kadak, Mehmet Gürkan
Fattening Performance And Carcass Characteristics Of Awassi Crosses (F1 And B1) With German Blackheaded Mutton And Hampshire Down Male Lambs, Ali̇ Akmaz, Emi̇n Teki̇n, Ramazan Kadak, Mehmet Gürkan
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to investigate the fattening and carcass characteristics of German Blackheaded Mutton (GBM) x Awassi (A) F1, Hampshire Down (HD) x A (F1) and HD x (HD x A) B1 male lambs. Nine GBM x A (F1), eight (HD) x A (F1) and eight HD x (HD x A) B1 male lambs were used for fattening traits, and six of each breed for carcass traits. The lambs were fed, from the beginning at approximately 20 kg of live weights to the finish at 45 kg, alfalfa hay 100 g/head/day and concentrates ad libitum. For statistical analysis, …
Effects Of Vitamins A, E And Selenium On Reproductive Performance And Serum Levels Ofsome Biochemical Parameters In Ewes, And Birth Weight And Survival Rates In Their Lambs, Mehmet Avci, Zi̇ya Karakilçik, Rahmi̇ Kanat
Effects Of Vitamins A, E And Selenium On Reproductive Performance And Serum Levels Ofsome Biochemical Parameters In Ewes, And Birth Weight And Survival Rates In Their Lambs, Mehmet Avci, Zi̇ya Karakilçik, Rahmi̇ Kanat
Turkish Journal of Veterinary & Animal Sciences
It has been reported that vitamins A and E and Se affected the reproductive performance and some biochemical parameters in ewes, and the numbers, concentration and motility of spermatozoa in rams. In addition, Se and vitamin E have important functions in the etiology of WMD. Therefore, this study was designed to investigate the effects of vitamin A and E and Se supplementation on reproductive performance and some biochemical parameters in ewes, and birth weight and survival rates of lambs. Four groups were formed (A, B, C, D) and fed with a vitamin A, E and Se supplemented diet as follows. …
The Possibilities Of Developing Dam And Sire Lines Using Akkaraman, Sakız And Kıvırcıksheep Breeds For Lamb Productioni. Fertility In Akkaraman Sheep, Survival Rate And Growth Characteristics Of Sakız Xakkaraman F_1 And Kıvırcık X Akkaraman F_1 Lambs, Hali̇l Akçapinar, Ceyhan Özbeyaz, Necmetti̇n Ünal, Mustafa Avci
The Possibilities Of Developing Dam And Sire Lines Using Akkaraman, Sakız And Kıvırcıksheep Breeds For Lamb Productioni. Fertility In Akkaraman Sheep, Survival Rate And Growth Characteristics Of Sakız Xakkaraman F_1 And Kıvırcık X Akkaraman F_1 Lambs, Hali̇l Akçapinar, Ceyhan Özbeyaz, Necmetti̇n Ünal, Mustafa Avci
Turkish Journal of Veterinary & Animal Sciences
This study was conducted to investigate the possibilities of developing terminal male and female lines for lamb production by the crossing of native breeds, that is, Akkaraman, Sakız (Chios) and Kıvırcık breeds. The Akkaraman sheep is a fattailed breed raised in central Anatolia, while Sakız and Kıvırcık sheep aren non fattailed breeds raised in western and northern-western Anatolia. The Birth rate of Akkaraman x Akkaraman (AA), Sakız x Akkaraman (SA) and Kıvırcık x Akkaraman (KA) breeding groups were generally found as follows: 94.0, 78.6 and 74.0% in 1996; and 93.3, 90.8 and 85.0% in 1997, respectively. The survival rate of …
The Effect Of Amitraz Poisoning On Formation Of Methemoglobin And Hemolytic Anemia In Guinea-Pigs, Meltem Şi̇reli̇, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Ayhan Fi̇lazi̇
The Effect Of Amitraz Poisoning On Formation Of Methemoglobin And Hemolytic Anemia In Guinea-Pigs, Meltem Şi̇reli̇, Vedat Sağmanligi̇l, İlksi̇n Pi̇şki̇n, Ayhan Fi̇lazi̇
Turkish Journal of Veterinary & Animal Sciences
This investigation was carried out to determine the effect of amitraz poisoning on the the formation of methemoglobin and osmotic fragility of erytrocytes. in this study, 12 guinea-pigs, weighing 250-300 gr were used. They were equally divided into two groups, control and experimental. The animals in the experimental group were intraperitoneally injected with amitraz at a dose of 1 mg/kg body weight. One hour after amitraz injections, erytrocyte, hematocrit, hemoglobin levels and also percent hemolysis of erytrocytes were measured in the blood samples taken from the control and experimental groups. In the experimental group, the value of hemoglobin was lower …
Acute Phase Effect Of Trichloroethylene Ingestion On Some Biological Markers In Dogs, Recep Aslan, Mehmet Tütüncü, S. Oktay Aslan, Yilmaz Dündar
Acute Phase Effect Of Trichloroethylene Ingestion On Some Biological Markers In Dogs, Recep Aslan, Mehmet Tütüncü, S. Oktay Aslan, Yilmaz Dündar
Turkish Journal of Veterinary & Animal Sciences
Trichloroethylene (TE) is an environmental toxic solvent hazardous to human and domestic animals and well known in the industrial sector. The purpose of this study was to determine whether oral TE plays a role in lipid oxidation and tissue damage. Fourteen dogs were treated with an oral toxic dose of 0.5 ml/kg T.E. The acute changes that occurred in creatine kimase (CK), malondialdehyte (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were investigated in blood samples taken before application and 8, 24, 48, 72 and 168 hours after treatment. SOD and GSH-Px activities decreased, protein, albumin and alcalen phosphate did …
Use Of Elisa, If A, And A Vidin-Biotin Staining For The Diagnosis Of Bovine Genital Campylobacteriosis, Seyyal Ak, Nuri̇ Turan, Kemal Ak, İ. Kamuran İleri̇, Ati̇lla Ilgaz
Use Of Elisa, If A, And A Vidin-Biotin Staining For The Diagnosis Of Bovine Genital Campylobacteriosis, Seyyal Ak, Nuri̇ Turan, Kemal Ak, İ. Kamuran İleri̇, Ati̇lla Ilgaz
Turkish Journal of Veterinary & Animal Sciences
Acid glycine extract (AGE) and whole cell (WC) antibodies wer e used in ELISA testing for the detection of Campylobacter fetus subsp. venerealis and subsp. fetus antibodies in the blood sera of 150 female cattle and 100 bulls which wer e one year old or older . It was observed that AGE antibody was mor e specific than WC antibody . A cr oss r eaction was detected between C. fetus subsp. venerealis and subsp. fetus. Thus, it was concluded that for Campylobacter ELISA could only be used as a monitoring test. In dir ect diagnosis of C. fetus with …
Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz
Detection Of Leptospira Species By Polymerase Chain Reaction (Pcr) In Urine Of Cattle, Burhan Çeti̇nkaya, Hasan Basri̇ Ertaş, Hasan Öngör, Adi̇le Muz
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to investigate the prevalance of leptospirosis in the urine of cattle slaughtered in three major abattoirs in the east of Turkey. A polymerase chain reaction (PCR) based on a pair of genus-specific primers was used to detect leptospiral DNA in the urine samples of 473 cattle, 284 of which were from Elazig, 112 from Malatya and 77 from Diyarbakir. The detection limit of the method was determined to be approximately five bacteria per ml of urine. In the examination of urine samples, 4.02% (19/473) (95% confidence intervals [Cl] 2.4-6.2) were found to be positive by …
Development Of An Enzyme Immunoassay For The Determination Of Ovine Growth Hormone In Plasma, Behi̇ç Serpek, Seyfullah Hali̇loğlu
Development Of An Enzyme Immunoassay For The Determination Of Ovine Growth Hormone In Plasma, Behi̇ç Serpek, Seyfullah Hali̇loğlu
Turkish Journal of Veterinary & Animal Sciences
A competitive enzyme immunoassay for the determination of ovine growth hormone (oGH) was developed. The assay is based on (anti-rabbit IgG) goat IgG-coated microtitreplates, anti-oGH from rabbits, biotinyl-oGH and streptavidin-horseradish peroxidase. The assay is done directly with 40 ml of plasma and the calibration graph (90% relative binding at 0.38 ± 0.02 ng ml -1 and 50% relative binding at 3.80 ± 0.18 ng ml -1 ) is prepared in plasma without measurable endogenous oGH. Recovery in different plasma samples with added oGH amounted to 96.78 - 100.03 %, and all variabilities were < 10.44. The assay shows no significant binding of oFSH, oLH, oTSH and oPRL. The assay detects high and low plasma oGH levels within the physiological variation as well as changes in plasma oGH after stimulation with growth hormone releasing factor. Hence, it offers a reliable alternative radioimmunoassay. However, a reference method providing evidence of the identity of exogenous oGH will be required for proving illegal treatment with oGH and for forensic purposes.
Localization Of Haemophilus Somnus Antigen By An Immunoper Oxidase Technique In Pneumonic Bovine Lungs, Rifki Haziroğlu, Jale Erdeğer, M. Yavuz Gülbahar, Oğuz Kul, Murat Yildirim
Localization Of Haemophilus Somnus Antigen By An Immunoper Oxidase Technique In Pneumonic Bovine Lungs, Rifki Haziroğlu, Jale Erdeğer, M. Yavuz Gülbahar, Oğuz Kul, Murat Yildirim
Turkish Journal of Veterinary & Animal Sciences
One hundred pneumonic lungs from calves were examined by an immunoper oxidase technique using avidin-biotin- per oxidase complex method in formalin-fixed, paraffin-embedded sections for Haemophilus somnus. In the histological examination of tissue sections, strong positive reactions against H. somnus were detected in 14% of the lungs. H. somnus antigens were observed at the surface and/or within the epithelial cells, macrophages, necrotic alveolar walls, alveolar -bronchial/br onchiolar exudates, fibrins, distended interlobular septa and perivascular areas. In addition, dense antigen accumulations were detected in the necrotic macrophages and neutrophils.
Cover And Contents
Turkish Journal of Veterinary & Animal Sciences
No abstract provided.
Living In Seyhan Dam Lake And Seyhan River Determination Of Some Blood Parameters In Mirror Carps ( Cyprinus Carpio, Linnaeus, 1758), İbrahi̇m Cengi̇zler, Aysel Şahan Azi̇zoğlu
Living In Seyhan Dam Lake And Seyhan River Determination Of Some Blood Parameters In Mirror Carps ( Cyprinus Carpio, Linnaeus, 1758), İbrahi̇m Cengi̇zler, Aysel Şahan Azi̇zoğlu
Turkish Journal of Veterinary & Animal Sciences
This research was carried out on mirror carps ( Cyprinus carpio, L.,1758) taken from Seyhan Dam Lake and Seyhan River between January and October 1996 to determine some blood parameters. Erythrocyte, leucocyte count, leucocyte cell formulas, total serum protein and serum glucose values of mirror Carps ( Cyprinus carpio, L.,1758 ) caught from Seyhan Dam Lake and Seyhan River were also compared statistically. Different values were recorded in erythrocyte and serum protein amounts for both environments (P0.05).