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Cefoperazone-Sulbactam Plus Amikacin Empirical Therapy For Febrile Neutropenia In Children With Cancer, İbrahi̇m Bayram, Fati̇h Erbey, Derya Alabaz, Sema Yilmaz, Ati̇la Tanyeli̇ Jan 2009

Cefoperazone-Sulbactam Plus Amikacin Empirical Therapy For Febrile Neutropenia In Children With Cancer, İbrahi̇m Bayram, Fati̇h Erbey, Derya Alabaz, Sema Yilmaz, Ati̇la Tanyeli̇

Turkish Journal of Medical Sciences

Aim: To determine the efficacy and safety of cefoperazone-sulbactam combined with amikacin in the treatment of febrile neutropenia in children with cancer. Materials and Methods: The study included 20 cancer cases with 26 febrile neutropenia (FEN) episodes. Patient selection criteria were defined according to the guidelines issued by the Infectious Disease Society of America (IDSA). Results: Patients diagnosed with acute leukemia (58%) and solid tumors (42%) were recorded. Twelve (46.2%) of the primary disease cases were refractory. The number of infection episodes identified microbiologically and clinically was 10 (38.5%) and 12 (46.1%), respectively. Fever of unknown origin was observed in …


An Analysis Of Adult Patients With Pulmonary And Extrapulmonary Tuberculosis*, Aygün Öztop, İpek Ünsal, Türkan Günay, Vi̇ldan Avkan Oğuz, Riza Çakmak Jan 2009

An Analysis Of Adult Patients With Pulmonary And Extrapulmonary Tuberculosis*, Aygün Öztop, İpek Ünsal, Türkan Günay, Vi̇ldan Avkan Oğuz, Riza Çakmak

Turkish Journal of Medical Sciences

Aim: In this study, it was planned to compare the features of pulmonary and extra-pulmonary tuberculosis patients over age 15 years. Materials and methods: We compared 360 newly diagnosed pulmonary tuberculosis (PTB) and extrapulmonary tuberculosis (EPTB) patients who were treated and followed up in Kahramanlar Tuberculosis Dispensary between 2000 and 2003, and were older than 15 years of age. The medical records of both patient groups were investigated in terms of age, sex, accompanying diseases, contact history and means of diagnosis. Results: The data were compared statistically between the 2 groups. Of these patients 78.1% were diagnosed with PTB while …


Epidemiological Risk Factors For Crimean-Congo Hemorrhagic Fever Patients, Ayten Kadanali, Serpi̇l Erol, Zülal Özkurt, Kemaletti̇n Özden Jan 2009

Epidemiological Risk Factors For Crimean-Congo Hemorrhagic Fever Patients, Ayten Kadanali, Serpi̇l Erol, Zülal Özkurt, Kemaletti̇n Özden

Turkish Journal of Medical Sciences

Aim The aim of this study was to evaluate the epidemiological risk factors for Crimean-Congo hemorrhagic fever (CCHF) patients admitted to our hospitals between January 2004- December 2006. Materials and methods: This was a study of 63 CCHF patients whose definitive diagnosis was based on the detection of CCHF virus-specific IgM by ELISA and/or of genomic segments of the CCHF virus by real time polymerase chain reaction (RT-PCR) and 50 healthy controls. Related data were collected prospectively. Results: Twenty-two (50.8%) of the patients were female, and 31 of them (49.2%) were male. The mean age of the patients was 46 …


The Predictive Role Of Ast Level, Prothrombin Time, And Platelet Count In The Detection Of Liver Fibrosis In Patients With Chronic Hepatitis C, Özlem Kandemi̇r, Gürbüz Polat, Güli̇z Saraçoğlu, Bahar Taşdelen Jan 2009

The Predictive Role Of Ast Level, Prothrombin Time, And Platelet Count In The Detection Of Liver Fibrosis In Patients With Chronic Hepatitis C, Özlem Kandemi̇r, Gürbüz Polat, Güli̇z Saraçoğlu, Bahar Taşdelen

Turkish Journal of Medical Sciences

Aim: We aimed to assess the predictive value of a combination some of basic serum biochemical markers for the diagnosis of clinically significant fibrosis in chronic hepatitis C. Materials and methods: Blood samples from 68 patients with chronic hepatitis C infection were analyzed for aspartate aminotransferase, prothrombin INR, and platelet count. The liver histopathology was evaluated using the Knodell protocol. The relationship between the serum biochemical markers and severity of the stages were examined. Results: A strong association was found between the fibrosis stage and platelet count and GUCI (P < 0.001, P = 0.035 respectively) but not APRI ([AST/ULN] / Platelet count [ ×109 /L]) × 100) (P = 0.052). The relationship between the platelet count


Evaluation Of Ribavirin Genotoxicity With Sister Chromatid Exchange And Micronuclei Assays In Humans, Abdulgani̇ Tatar, Zülal Özkurt, Ahmet Hacimüftüoğlu, Ahmet Yeşi̇lyurt, Serhat Vançeli̇k Jan 2009

Evaluation Of Ribavirin Genotoxicity With Sister Chromatid Exchange And Micronuclei Assays In Humans, Abdulgani̇ Tatar, Zülal Özkurt, Ahmet Hacimüftüoğlu, Ahmet Yeşi̇lyurt, Serhat Vançeli̇k

Turkish Journal of Medical Sciences

Aim: The main objective of this study was to assess the genotoxicity of ribavirin in 15 patients who were suffering from Crimean-Congo haemorrhagic fever and treated with the therapy dose of the antiviral agent. Materials and Methods: Genotoxicity was evaluated using the cytokinesis-blocked micronucleus (MN) and sister chromatid exchange (SCE) assays in lymphocyte cultures that were prepared from blood samples collected from the 15 patients. The blood samples were taken on day 9 of a 10-day therapeutic regimen of ribavirin and 1 month after the cessation of therapy. Results: In all patients, the frequency of sister chromatid exchanges and the …


Coxiella Burnetii Seroprevalence In The Rural Part Of Bolu, Turkey*, Oğuz Karabay, Esra Koçoğlu, Gökhan Baysoy, Selami̇ Konyalioğlu Jan 2009

Coxiella Burnetii Seroprevalence In The Rural Part Of Bolu, Turkey*, Oğuz Karabay, Esra Koçoğlu, Gökhan Baysoy, Selami̇ Konyalioğlu

Turkish Journal of Medical Sciences

Aim: This study was performed to determine the seroprevalence of Coxiella burnetii in the rural part of Bolu, a city located in the western part of the Black Sea region of Turkey. Materials and Methods: The study population was chosen by cluster sampling to represent the rural population of the city. Serum samples were collected from the 9 rural districts of Bolu. Blood samples were taken to the laboratory while stored at 4 °C in ice-cooled boxes. The sera were then stored at -20 °C until testing. The presence of IgG antibodies against C. burnetii phase-II antigens in the sera …


The Risk Factors And Spread Of Multidrug-Resistant Acinetobacter Baumannii In Intubated Patients In A Medical Intensive Care Unit, Emi̇ne Alp, Mehmet Yerer, Sesi̇n Kocagöz, Gökhan Metan, Duygu Esel, Yeşi̇m Gürol, Mehmet Doğanay Jan 2009

The Risk Factors And Spread Of Multidrug-Resistant Acinetobacter Baumannii In Intubated Patients In A Medical Intensive Care Unit, Emi̇ne Alp, Mehmet Yerer, Sesi̇n Kocagöz, Gökhan Metan, Duygu Esel, Yeşi̇m Gürol, Mehmet Doğanay

Turkish Journal of Medical Sciences

Aim: Acinetobacter baumannii is one of the most common causes of ventilator-associated pneumonia in intensive care units. The aim of this study was to determine the risk factors for colonization in the respiratory tract and infection with A. baumannii in a medical intensive care unit (MICU), and to examine the genetic link between strains and the spread of isolates. Materials and methods: This study was conducted prospectively between 1 December 2004 and 31 January 2006 in the MICU. Patients (> 16 years old) admitted to the MICU that were mechanically ventilated and/or intubated were enrolled in the study. Results: Ninety-eight …


Risk Factors For Acquisition Of Methicillin-Resistant Staphylococcus Aureus And Clonal Spread Of The Isolates In A Medical Intensive Care Unit, Emi̇ne Alp, Mehmet Yerer, Duygu Esel, Gökhan Metan, Mehmet Doğanay Jan 2009

Risk Factors For Acquisition Of Methicillin-Resistant Staphylococcus Aureus And Clonal Spread Of The Isolates In A Medical Intensive Care Unit, Emi̇ne Alp, Mehmet Yerer, Duygu Esel, Gökhan Metan, Mehmet Doğanay

Turkish Journal of Medical Sciences

Aim: Methicillin-resistant Staphylococcus aureus (MRSA) is still the commonest pathogen in hospital-acquired infections with high morbidity and mortality. MRSA colonization usually precedes infection and dissemination of the microorganism. The aim of this study was to determine risk factors for the colonization and infection with MRSA in a medical intensive care unit (MICU) and to show the genetic relation of strains. Materials and methods: This study was conducted prospectively between 1 December 2004 and 31 January 2006 in MICU. Patients (>16 years) admitted to the MICU were screened for MRSA on admission (in the first 48 hours), at the end …


In Vitro Activity Of Tigecycline Against Acinetobacter Baumannii Strains Isolated From Nosocomial Infections, Esragül Akinci, İpek Mumcuoğlu, Pinar Öngürü, F. Nurhayat Bayazit, Sevi̇nç Ersoy Şen, Ayşe Erbay, Neri̇man Balaban, Hürrem Bodur Jan 2008

In Vitro Activity Of Tigecycline Against Acinetobacter Baumannii Strains Isolated From Nosocomial Infections, Esragül Akinci, İpek Mumcuoğlu, Pinar Öngürü, F. Nurhayat Bayazit, Sevi̇nç Ersoy Şen, Ayşe Erbay, Neri̇man Balaban, Hürrem Bodur

Turkish Journal of Medical Sciences

Aims: Acinetobacter species usually affect hospitalized patients and are involved in many infections associated with a high mortality rate. Management of infections caused by these strains is very difficult, as the strains often display multiple drug resistance, including carbapenem resistance. Tigecycline is the most recently developed semi-synthetic broad spectrum glycylcycline antibiotic. The present study was conducted to evaluate the in vitro susceptibility of Acinetobacter baumannii strains isolated from the hospitalized patients to tigecycline. Materials and Methods: Acinetobacter isolates were collected from hospitalized patients with documented nosocomial infections between June 2005 and October 2006. Only one isolate per patient was accepted. …


Surveillance Of Nosocomial Infections In Dicle University Hospital: A Ten-Year Experience, Mehmet Faruk Geyi̇k, Sali̇h Hoşoğlu, Celal Ayaz, Mustafa Kemal Çelen, Cemal Üstün Jan 2008

Surveillance Of Nosocomial Infections In Dicle University Hospital: A Ten-Year Experience, Mehmet Faruk Geyi̇k, Sali̇h Hoşoğlu, Celal Ayaz, Mustafa Kemal Çelen, Cemal Üstün

Turkish Journal of Medical Sciences

Aim: The main objective was to recognize the evaluation of surveillance program on the nosocomial infections (NIs) in Dicle University Hospital (DUH) Materials and Methods: A prevalence study was performed prospectively, at the DUH from 1997 to 2006. Active surveillance for NIs were performed by infection control team, using the criteria proposed by the Centers for Diseases Control and Prevention (CDC) and National Nosocomial Infections Surveillance System (NNIS) methodology. This team includes infection control doctor and two nurses, who visited hospital units three times a week. All cases with NI were recorded using a standard data collection form. Results: During …


Survival Of Salmonella Typhi And Shigella Flexneri In Different Water Samples And At Different Temperatures, M. Hami̇dullah Uyanik, Hali̇l Yazgi, Ahmet Ayyildiz Jan 2008

Survival Of Salmonella Typhi And Shigella Flexneri In Different Water Samples And At Different Temperatures, M. Hami̇dullah Uyanik, Hali̇l Yazgi, Ahmet Ayyildiz

Turkish Journal of Medical Sciences

Aim: In this study, we aimed to evaluate the survival of Salmonella typhi and Shigella flexneri, which have significance for public health, in various aqueous media (distilled water, 0.9% NaCl solution, chlorinated water) and at different temperatures. Materials and Methods: The water samples mentioned above were contaminated with approximately 5 x 10^5 bacteria/ml of S. typhi and S. flexneri, and then stored at 37 ºC, room temperature, in refrigerator (4 to 6 ºC) and in deep freezer (-20 ºC). From the water materials prepared accordingly, cultures were done beginning in the first 24 hours and later every other day for …


Hematological Complications In 787 Cases Of Acute Brucellosis In Eastern Turkey, İmdat Di̇lek, Ahmet Durmuş, Mustafa Kasim Karahocagi̇l, Hayretti̇n Akdeni̇z, Hasan Karsen, Ali̇ İrfan Baran, Ömer Evi̇rgen Jan 2008

Hematological Complications In 787 Cases Of Acute Brucellosis In Eastern Turkey, İmdat Di̇lek, Ahmet Durmuş, Mustafa Kasim Karahocagi̇l, Hayretti̇n Akdeni̇z, Hasan Karsen, Ali̇ İrfan Baran, Ömer Evi̇rgen

Turkish Journal of Medical Sciences

Aim: In this paper, we present the hematological findings of 787 cases diagnosed with acute brucellosis. Materials and Methods: Records of the cases seen between 1994 and 2006 were retrospectively investigated regarding hematological changes. Results: Three hundred sixty-five (46%) patients were male and 422 (54%) were female. Mean age was 32 (range: 11-78 years). Mean hematological values were as follows: Hb 12 g/dl (4-19), Htc 36% (12-56), leukocyte 6.9 x 10^9/L (0.5-25) and platelets 213 x 10^9/L (9-617). According to differential counts, 34% and 14% of patients had mononuclear cell and neutrophil dominance, respectively. Fifty-six percent of patients were found …


A Study Of Learning Assessment Of Personal Hygiene Skills Of Mentally Retarded Individuals In Drop-In Day Care Services, Fi̇gen Ar, Esra Kiliç, Ayşegül Akbay Yarpuzlu Jan 2008

A Study Of Learning Assessment Of Personal Hygiene Skills Of Mentally Retarded Individuals In Drop-In Day Care Services, Fi̇gen Ar, Esra Kiliç, Ayşegül Akbay Yarpuzlu

Turkish Journal of Medical Sciences

Aim: The aim of the study was to assess the success and efficiency of the training practices for acquisition of basic personal hygiene skills and behavior of hand washing and soiling in a relatively heterogeneous group of individuals receiving care from a drop-in day care/special education service. We also analyzed etiologies of the causes of discrepancies in learning behavior implying a need for differentiation of intellectually disabled individuals who are candidates for continuous long-term institutional medical care from those who can be integrated into the community through special education. Materials and Methods: The study included 30 participants with mental retardation …


Clinical And Rhinoscintigraphic Evaluation Of Oral Macrolides In The Management Of Sinonasal Polyposis, Emi̇ne Aydin, Hülya Ortapamuk, Hakan Korkmaz, Engi̇n Dursun, Adi̇l Eryilmaz, Güleser Saylam, Muharrem Dağli Jan 2008

Clinical And Rhinoscintigraphic Evaluation Of Oral Macrolides In The Management Of Sinonasal Polyposis, Emi̇ne Aydin, Hülya Ortapamuk, Hakan Korkmaz, Engi̇n Dursun, Adi̇l Eryilmaz, Güleser Saylam, Muharrem Dağli

Turkish Journal of Medical Sciences

Aim: To document the efficacy of low-dose long-term oral macrolide treatment in the management of sinonasal polyposis. The study was designed prospectively in a group of patients with sinonasal polyposis and negative history of surgery. Materials and Methods: Ten patients were administered roxithromycin (RXM) 150 mg/day single oral dose for eight weeks. All patients underwent Tc-99m rhinoscintigraphic evaluation of mucociliary activity and endoscopic evaluation before and after the treatments. Response to treatment, in terms of rhinoscintigraphic and endoscopic improvement, was assessed. Results: Of the 10 patients, 7 were male and 3 female, with an average age of 46 years (27-74). …


Cover And Contents Jan 2007

Cover And Contents

Turkish Journal of Medical Sciences

No abstract provided.


Spectrum Of Clinical Infectious Diseases In Hospitalized Elderly Patients In The Southeast Of Iran, Batool Sharifi-Mood, Maliheh Metanat Jan 2007

Spectrum Of Clinical Infectious Diseases In Hospitalized Elderly Patients In The Southeast Of Iran, Batool Sharifi-Mood, Maliheh Metanat

Turkish Journal of Medical Sciences

Aim: The elderly have defective host defenses that compromise their ability to ward off infectious agents; factors influencing immunocompetence include impaired host defenses such as diminished cell-mediated immunity, changes in chronic diseases, medications, malnutrition and functional impairments. This study was conducted in order to determine the pattern of clinical infectious diseases in a series of hospitalized elderly patients. Materials and Methods: In this cross-sectional study, all elderly patients who were admitted to our hospital in Zahedan (Southeast Iran) between 2001 and 2006 were evaluated. We retrospectively reviewed the 1278 patients for diagnostic studies, risk factors, treatment options and morbidity and …


Complications Of Brucella Infection Among Adults: An 18-Year Retrospective Evaluation, Mustafa Ertek, Hali̇l Yazgi, Ayten Kadanali, Kemaletti̇n Özden, Mehmet Akin Taşyaran Jan 2006

Complications Of Brucella Infection Among Adults: An 18-Year Retrospective Evaluation, Mustafa Ertek, Hali̇l Yazgi, Ayten Kadanali, Kemaletti̇n Özden, Mehmet Akin Taşyaran

Turkish Journal of Medical Sciences

Aim: Brucellosis is a systemic infection in which any organ or system of the body can be involved. We evaluate here complications and involvement of Brucella infection in Erzurum, Turkey. Materials and Methods: We analyzed retrospectively 216 adult (89 female and 127 male) cases of brucellosis who were diagnosed and treated in the Infectious Disease Clinic of Atatürk University Research Hospital from 1985 to 2002. The diagnosis was based on clinical findings and serologic results and/or blood culture confirmations. Results: All isolates from cultures were identified as Brucella melitensis biotype 1. Skeletal complications were the most frequent, found in 147 …


Ebola: A Significant Threat As An Infectious Disease, And As A Potential Bioterrorism Agent, Çağatay Üstün, Özge Özgürler Jan 2005

Ebola: A Significant Threat As An Infectious Disease, And As A Potential Bioterrorism Agent, Çağatay Üstün, Özge Özgürler

Turkish Journal of Medical Sciences

No abstract provided.


Gunshot Wounds And Late Infection, Orhan Yildiz, Emi̇ne Alp, Serap Şi̇mşek, Ahmet Selçuklu, Mehmet Doğanay Jan 2005

Gunshot Wounds And Late Infection, Orhan Yildiz, Emi̇ne Alp, Serap Şi̇mşek, Ahmet Selçuklu, Mehmet Doğanay

Turkish Journal of Medical Sciences

LDIZ^1, Emine ALP^1, Serap ŞİMŞEK^1, Ahmet SELÇUKLU^2, Mehmet DOĞANAY^2 ^1Department of Infectious Diseases, Faculty of Medicine, Erciyes University, Kayseri - Turkey ^2Department of Neurosurgery, Faculty of Medicine, Erciyes University, Kayseri - Turkey


Pelvic Aspergillosis In A Renal Transplant Patient, Mustafa Sofi̇keri̇m, Yeşi̇m Çeti̇nkaya, Fuad Guli̇yev, Banu Sancak, Ender Özden, İlhan Erkan, Mehmet Bakkaloğlu Jan 2005

Pelvic Aspergillosis In A Renal Transplant Patient, Mustafa Sofi̇keri̇m, Yeşi̇m Çeti̇nkaya, Fuad Guli̇yev, Banu Sancak, Ender Özden, İlhan Erkan, Mehmet Bakkaloğlu

Turkish Journal of Medical Sciences

SOFİKERİM^1, Yeşim ÇETİNKAYA^2, Fuad GULİYEV^1, Banu SANCAK3, Ender ÖZDEN^1, İlhan ERKAN^1, Mehmet BAKKALOĞLU^1 ^1Department of Urology, Faculty of Medicine, Hacettepe University, Ankara - Turkey ^2Department of Internal Medicine, Section of Infectious Diseases, Faculty of Medicine, Hacettepe University, Ankara - Turkey ^3Department of Microbiology and Clinical Microbiology, Faculty of Medicine, Hacettepe University, Ankara, Turkey


Fatal Liver Failure Secondary To Chemotherapy Induced Hepatitis-B Virus Reactivation In A Patient With Acute Myeloid Leukemia, Zülal Özkurt, Mustafa Ertek, Ayten Kadanali, Serpi̇l Erol, Mehmet Parlak Jan 2005

Fatal Liver Failure Secondary To Chemotherapy Induced Hepatitis-B Virus Reactivation In A Patient With Acute Myeloid Leukemia, Zülal Özkurt, Mustafa Ertek, Ayten Kadanali, Serpi̇l Erol, Mehmet Parlak

Turkish Journal of Medical Sciences

KURT, Mustafa ERTEK, Ayten KADANALI, Serpil EROL, Mehmet PARLAK Department of Clinical Bacteriology and Infectious Diseases, Faculty of Medicine, Atatürk University, Erzurum - Turkey


Cover And Contents Jan 2005

Cover And Contents

Turkish Journal of Medical Sciences

No abstract provided.


Acute Hepatic Failure In A Case Of Acute Lymphoblastic Leukemia, Sali̇h Cesur, Pervi̇n Topçuoğlu, Ozan Aşik, Serhat Bi̇rengel, Muhi̇t Özcan Jan 2004

Acute Hepatic Failure In A Case Of Acute Lymphoblastic Leukemia, Sali̇h Cesur, Pervi̇n Topçuoğlu, Ozan Aşik, Serhat Bi̇rengel, Muhi̇t Özcan

Turkish Journal of Medical Sciences

SUR^1, Pervin TOPÇUOĞLU^2, Ozan AŞIK^3, Serhat BİRENGEL^3 ,Muhit ÖZCAN^2 ^1Refik Saydam Hygiene Center Tuberculosis Reference Laboratory, Ankara – Turkey ^2 Department of Hematology, Faculty of Medicine, Ankara University, Ankara - Turkey ^3 Department of Infectious Diseases and Clinical Bacteriology, Faculty of Medicine, Ankara University, Ankara - Turkey


Severe Acute Respiratory Syndrome (Sars): The First Pandemic Of The New Century, Murat Akova Jan 2003

Severe Acute Respiratory Syndrome (Sars): The First Pandemic Of The New Century, Murat Akova

Turkish Journal of Medical Sciences

No abstract provided.


Multiple Infected Cerebral Hydatid Cysts Treated With Albendazole, Mehmet Dumlu Aydin, Mustafa Ertek, Hali̇l Yazgi Jan 2003

Multiple Infected Cerebral Hydatid Cysts Treated With Albendazole, Mehmet Dumlu Aydin, Mustafa Ertek, Hali̇l Yazgi

Turkish Journal of Medical Sciences

umlu AYDIN^1, Mustafa ERTEK^2, Halil YAZGI^3 ^1Department of Neurosurgery, Faculty of Medicine, Atatürk University, Erzurum - Turkey ^2Department of Infectious Disease, Faculty of Medicine, Atatürk University, Erzurum - Turkey 3Department of Clinical Microbiology, Faculty of Medicine, Atatürk University, Erzurum - Turkey


Haemophilus Parainfluenzae Endocarditis, Emi̇ne Alp, Orhan Yildiz, Bi̇lgehan Aygen, Bülent Sümerkan Jan 2003

Haemophilus Parainfluenzae Endocarditis, Emi̇ne Alp, Orhan Yildiz, Bi̇lgehan Aygen, Bülent Sümerkan

Turkish Journal of Medical Sciences

No abstract provided.


Psoas Abscess Secondary To Brucellar Spondylitis, Bi̇lgehan Aygen, Duygu Eşel, Nuri̇ Erdoğan, Orhan Yildiz, Bülent Sümerkan, Mehmet Doğanay Jan 2002

Psoas Abscess Secondary To Brucellar Spondylitis, Bi̇lgehan Aygen, Duygu Eşel, Nuri̇ Erdoğan, Orhan Yildiz, Bülent Sümerkan, Mehmet Doğanay

Turkish Journal of Medical Sciences

No abstract provided.


Isolated Hydatic Cyst In The Subcutaneous Tissue, Tanju Acar, Ramazan Taçyildiz, Sali̇h Tuncal Jan 2001

Isolated Hydatic Cyst In The Subcutaneous Tissue, Tanju Acar, Ramazan Taçyildiz, Sali̇h Tuncal

Turkish Journal of Medical Sciences

No abstract provided.


Production And Characterization Of Monoclonal Anti-Ovalbumin Antibodies, Mehmet Zi̇ya Doymaz, Hakan Bulut, Aykut Özdarendeli̇, Yusuf Bolat Jan 2000

Production And Characterization Of Monoclonal Anti-Ovalbumin Antibodies, Mehmet Zi̇ya Doymaz, Hakan Bulut, Aykut Özdarendeli̇, Yusuf Bolat

Turkish Journal of Medical Sciences

In this study, the results of anti- ovalbumin (OVA) monoclonal antibody production and characterization are reported. Splenocytes from OVA-hyperimmune Balb/C mouse were fused with F0 myeloma cells and resulting hybridoma cells were selected in hypoxanthine-aminopterine-thymidine containing medium. A total of 37 HAT resistant hybridomas were obtained. Upon testing the hybridoma supernatants in OVA and mouse immunoglobulin G specific enzyme-linked immunosorbent assays, we observed that two of the hybridomas secreted OVA specific antibodies. The hybridomas produced IgG class antibodies. The hybridomas were grown and the supernatants from the cultures were also tested in a Western Blot assay. The results of ELISA …


Non-Gestational Impetigo Herpetiformis, Teoman Erdem, Ali̇ Karakuzu, Muammer Parlak, Necmetti̇n Akdeni̇z Jan 1999

Non-Gestational Impetigo Herpetiformis, Teoman Erdem, Ali̇ Karakuzu, Muammer Parlak, Necmetti̇n Akdeni̇z

Turkish Journal of Medical Sciences

No abstract provided.